MD11G1216300.v1.1

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0013358
Physical Location & Seq
Reverse (-)
31577190 .. 31579529
2340 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1216300.v1.1.491

Sequence Viewer

Length: 831 bp
ATGAGACGAAACTCAGATGACAATGCTCCTAATTACTTTAGTGTCCTGAAGCTCATAGACTCCTCCCTCAGGATTTCTGCGATTGCTCTGAGCGTTGCAACCATTTGGCTCACTGTGACCAACCACCAAGACAACCCCAGCTATGGCAACCTAAAGTTCTCCAATTTCACGGGCCTCAAGTACATGGTTTGCATCAGCAACATTTCTGGTGTGCACGCTTTTCTTGTAGCTTTGTCCTCATGGATCACGTGCTTTGTGACTAAAGCTTGGCTTTTCTTTGTCTCTACCAGATTCGAAGCGAGAACTTTATGCCAAGCTATTAGAACTTCGTTCATCTGTGCAATATGGGCAGCAGTTAATAAATTTTGGTCGTTGGAGTTTGAGATTAGGCGGTGGGTGTGTCCGCGCGGTTGTGAGCTGCAGTTGTCCCTATGGCTCGGGCCACAGAAGAGCAAGCGTCGCCGATTGCATGGCCACGGTGACAGTGCCGTAGAAGAAAGAATGGAAAGCCCAGGGAATGGAAGTCGGGATTTAGCAAGGGGATGCCCGGGTCTTGCTGTCTGCCTCATTGTAGCTTATTTAATGGTCACATCTTGGGCGGCAGTGATGGAGATATTGTCTTTAGCTTACAAAGGGGACAAGACAGTCTCATGGAGTGAAGCCTGCACTTCTTATGGGAAGTTCTGCAGCAGAATGAAGGTGGCATTGGTTCTCCATGCCCTGGCTCTTTGTTGCTTTCTTGTCTTGGCTGTGATCTCAGCTTATAGGGTTTTCAGCATGTTTGAGCCACCTTCTGTTTCTGATAAGGAGGTGGAAGAAGAGAGGACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

277

Amino Acids

30.98

Weight (kDa)

8.93

Isoelectric Point (pI)

41.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 16 - 94 8.7e-10 Casparian strip membrane protein domain
CASP_dom PF04535 190 - 245 3.2e-10 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013358)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54980
fragaria_vesca FvH4_3g22910
malus_domestica MD03G1199400.v1.1 MD11G1216200.v1.1 MD11G1216300.v1.1
prunus_persica Prupe.4G215200_v2.0.a1
pyrus_communis pycom03g15100
rosa_chinensis RchiOBHm_Chr5g0040241
rosa_laevigata RLG00000033966
rosa_multiflora Rmu_sc0002908.1_g000012 Rmu_sc0010162.1_g000006
rosa_roxburghii Rroxscaffold_1G00041070
rosa_rugosa Rorug05G0184200
rosa_samantha Rh5AG271100 Rh5BG274400 Rh5CG307300 Rh5DG282600
rosa_wichuraiana Rw5G025350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 644
AccB7I CCANNNNNTGG 2 cut(s) 518, 721
AccII CGCG 2 cut(s) 406, 408
AciI CCGC 4 cut(s) 391, 404, 408, 599
AclWI GGATC 1 cut(s) 251
AcoI YGGCCR 1 cut(s) 472
AcsI RAATTY 1 cut(s) 362
AcuI CTGAAG 1 cut(s) 68
AcvI CACGTG 1 cut(s) 249
AfaI GTAC 1 cut(s) 182
AfiI CCNNNNNNNGG 4 cut(s) 69, 143, 518, 721
AjnI CCWGG 2 cut(s) 511, 720
AjuI GAANNNNNNNTTGG 2 cut(s) 689, 721
AluBI AGCT 9 cut(s) 52, 141, 230, 266, 317, 418, 575, 626, 761
AluI AGCT 9 cut(s) 52, 141, 230, 266, 317, 418, 575, 626, 761
Alw21I GWGCWC 1 cut(s) 216
Alw26I GTCTC 2 cut(s) 286, 652
Alw44I GTGCAC 1 cut(s) 212
AlwI GGATC 1 cut(s) 251
Ama87I CYCGRG 2 cut(s) 437, 547
AoxI GGCC 3 cut(s) 172, 440, 472
ApaLI GTGCAC 1 cut(s) 212
ApeKI GCWGC 3 cut(s) 350, 418, 687
ApoI RAATTY 1 cut(s) 362
AspLEI GCGC 1 cut(s) 408
AspS9I GGNCC 2 cut(s) 172, 440
AsuC2I CCSGG 2 cut(s) 548, 549
AsuHPI GGTGA 1 cut(s) 491
AsuII TTCGAA 1 cut(s) 294
AvaI CYCGRG 2 cut(s) 437, 547
AxyI CCTNAGG 1 cut(s) 68
BaeGI GKGCMC 1 cut(s) 216
BalI TGGCCA 1 cut(s) 474
BbrPI CACGTG 1 cut(s) 249
Bbv12I GWGCWC 1 cut(s) 216
BbvI GCAGC 3 cut(s) 362, 405, 699
BccI CCATC 1 cut(s) 601
BceAI ACGGC 1 cut(s) 473
BciT130I CCWGG 2 cut(s) 513, 722
BcnI CCSGG 2 cut(s) 548, 549
BcoDI GTCTC 2 cut(s) 286, 652
BfmI CTRYAG 2 cut(s) 419, 685
BisI GCNGC 4 cut(s) 351, 419, 600, 688
BlsI GCNGC 4 cut(s) 352, 420, 601, 689
Bme1390I CCNGG 4 cut(s) 513, 548, 549, 722
BmeT110I CYCGRG 2 cut(s) 437, 547
BmgT120I GGNCC 2 cut(s) 172, 440
BmrFI CCNGG 4 cut(s) 513, 548, 549, 722
BmsI GCATC 2 cut(s) 201, 533
Bpu14I TTCGAA 1 cut(s) 294
BpuEI CTTGAG 1 cut(s) 161
BpuMI CCSGG 2 cut(s) 548, 549
BsaAI YACGTR 1 cut(s) 249
BsaJI CCNNGG 5 cut(s) 475, 511, 512, 547, 720
BsaXI ACNNNNNCTCC 2 cut(s) 602, 632
Bsc4I CCNNNNNNNGG 4 cut(s) 69, 143, 518, 721
Bse21I CCTNAGG 1 cut(s) 68
BseBI CCWGG 2 cut(s) 513, 722
BseDI CCNNGG 5 cut(s) 475, 511, 512, 547, 720
BseGI GGATG 1 cut(s) 548
BseLI CCNNNNNNNGG 4 cut(s) 69, 143, 518, 721
BseMII CTCAG 4 cut(s) 27, 80, 82, 771
BseRI GAGGAG 1 cut(s) 52
BseSI GKGCMC 1 cut(s) 216
BseXI GCAGC 3 cut(s) 362, 405, 699
BseYI CCCAGC 1 cut(s) 137
BsgI GTGCAG 1 cut(s) 649
Bsh1236I CGCG 2 cut(s) 406, 408
BshFI GGCC 3 cut(s) 174, 442, 474
BsiHKAI GWGCWC 1 cut(s) 216
BsiHKCI CYCGRG 2 cut(s) 437, 547
BsiSI CCGG 1 cut(s) 548
BslFI GGGAC 2 cut(s) 412, 650
BslI CCNNNNNNNGG 4 cut(s) 69, 143, 518, 721
BsmAI GTCTC 2 cut(s) 286, 652
BsmFI GGGAC 2 cut(s) 412, 650
BsnI GGCC 3 cut(s) 174, 442, 474
BsoBI CYCGRG 2 cut(s) 437, 547
Bsp119I TTCGAA 1 cut(s) 294
Bsp1286I GDGCHC 1 cut(s) 216
Bsp143I GATC 2 cut(s) 243, 753
BspACI CCGC 4 cut(s) 391, 404, 408, 599
BspANI GGCC 3 cut(s) 174, 442, 474
BspCNI CTCAG 4 cut(s) 26, 81, 81, 770
BspFNI CGCG 2 cut(s) 406, 408
BspMAI CTGCAG 2 cut(s) 423, 689
BspPI GGATC 1 cut(s) 251
BspQI GCTCTTC 1 cut(s) 443
BspT104I TTCGAA 1 cut(s) 294
BssECI CCNNGG 5 cut(s) 475, 511, 512, 547, 720
BssMI GATC 2 cut(s) 243, 753
Bst2UI CCWGG 2 cut(s) 513, 722
Bst4CI ACNGT 4 cut(s) 115, 479, 485, 646
Bst6I CTCTTC 2 cut(s) 443, 813
BstBAI YACGTR 1 cut(s) 249
BstBI TTCGAA 1 cut(s) 294
BstC8I GCNNGC 3 cut(s) 216, 455, 664
BstDEI CTNAG 5 cut(s) 13, 68, 89, 757, 828
BstDSI CCRYGG 1 cut(s) 475
BstENI CCTNNNNNAGG 1 cut(s) 67
BstF5I GGATG 1 cut(s) 548
BstFNI CGCG 2 cut(s) 406, 408
BstHHI GCGC 1 cut(s) 408
BstKTI GATC 2 cut(s) 246, 756
BstMAI GTCTC 2 cut(s) 286, 652
BstMBI GATC 2 cut(s) 243, 753
BstMWI GCNNNNNNNGC 2 cut(s) 347, 459
BstNI CCWGG 2 cut(s) 513, 722
BstNSI RCATGY 1 cut(s) 781
BstSCI CCNGG 4 cut(s) 511, 546, 547, 720
BstSFI CTRYAG 2 cut(s) 419, 685
BstSLI GKGCMC 1 cut(s) 216
BstUI CGCG 2 cut(s) 406, 408
BstV1I GCAGC 3 cut(s) 362, 405, 699
Bsu36I CCTNAGG 1 cut(s) 68
BsuRI GGCC 3 cut(s) 174, 442, 474
BtgI CCRYGG 1 cut(s) 475
BtsCI GGATG 1 cut(s) 548
BtsI GCAGTG 1 cut(s) 609
BtsIMutI CAGTG 3 cut(s) 111, 490, 609
Cac8I GCNNGC 3 cut(s) 216, 455, 664
CfoI GCGC 1 cut(s) 408
Cfr13I GGNCC 2 cut(s) 172, 440
Cfr9I CCCGGG 1 cut(s) 547
CseI GACGC 1 cut(s) 446
Csp6I GTAC 1 cut(s) 181
CviAII CATG 6 cut(s) 184, 240, 470, 651, 716, 778
CviQI GTAC 1 cut(s) 181
DdeI CTNAG 5 cut(s) 13, 68, 89, 757, 828
DpnI GATC 2 cut(s) 245, 755
DpnII GATC 2 cut(s) 243, 753
DrdI GACNNNNNNGTC 1 cut(s) 644
DseDI GACNNNNNNGTC 1 cut(s) 644
EaeI YGGCCR 1 cut(s) 472
Eam1104I CTCTTC 2 cut(s) 443, 813
EarI CTCTTC 2 cut(s) 443, 813
Eco57I CTGAAG 1 cut(s) 68
Eco72I CACGTG 1 cut(s) 249
Eco81I CCTNAGG 1 cut(s) 68
Eco88I CYCGRG 2 cut(s) 437, 547
EcoNI CCTNNNNNAGG 1 cut(s) 67
EcoRII CCWGG 2 cut(s) 511, 720
FaeI CATG 6 cut(s) 187, 243, 473, 654, 719, 781
FalI AAGNNNNNCTT 2 cut(s) 255, 287
FaqI GGGAC 2 cut(s) 412, 650
FatI CATG 6 cut(s) 183, 239, 469, 650, 715, 777
Fnu4HI GCNGC 4 cut(s) 351, 419, 600, 688
FokI GGATG 1 cut(s) 555
Fsp4HI GCNGC 4 cut(s) 351, 419, 600, 688
GlaI GCGC 1 cut(s) 407
GluI GCNGC 4 cut(s) 351, 419, 600, 688
GsaI CCCAGC 1 cut(s) 141
HaeIII GGCC 3 cut(s) 174, 442, 474
HapII CCGG 1 cut(s) 548
HgaI GACGC 1 cut(s) 446
HhaI GCGC 1 cut(s) 408
Hin1II CATG 6 cut(s) 187, 243, 473, 654, 719, 781
Hin6I GCGC 1 cut(s) 406
HinP1I GCGC 1 cut(s) 406
HindIII AAGCTT 1 cut(s) 264
HinfI GANTC 2 cut(s) 59, 291
HpaII CCGG 1 cut(s) 548
HphI GGTGA 1 cut(s) 491
Hpy166II GTNNAC 1 cut(s) 214
Hpy188I TCNGA 3 cut(s) 16, 90, 802
Hpy188III TCNNGA 3 cut(s) 46, 70, 527
Hpy8I GTNNAC 1 cut(s) 214
Hpy99I CGWCG 1 cut(s) 462
HpyAV CCTTC 2 cut(s) 691, 801
HpyCH4III ACNGT 4 cut(s) 115, 479, 485, 646
HpyCH4IV ACGT 1 cut(s) 248
HpyCH4V TGCA 8 cut(s) 98, 192, 214, 341, 421, 469, 666, 687
HpyF10VI GCNNNNNNNGC 2 cut(s) 347, 459
HpyF3I CTNAG 5 cut(s) 13, 68, 89, 757, 828
HpySE526I ACGT 1 cut(s) 248
Hsp92II CATG 6 cut(s) 187, 243, 473, 654, 719, 781
HspAI GCGC 1 cut(s) 406
Kzo9I GATC 2 cut(s) 243, 753
LguI GCTCTTC 1 cut(s) 443
LmnI GCTCC 1 cut(s) 31
Lsp1109I GCAGC 3 cut(s) 362, 405, 699
LweI GCATC 2 cut(s) 201, 533
MaeII ACGT 1 cut(s) 248
MaeIII GTNAC 4 cut(s) 115, 256, 479, 586
MalI GATC 2 cut(s) 245, 755
MboI GATC 2 cut(s) 243, 753
MboII GAAGA 4 cut(s) 460, 506, 827, 830
MhlI GDGCHC 1 cut(s) 216
MlsI TGGCCA 1 cut(s) 474
MluCI AATT 3 cut(s) 31, 163, 362
MluNI TGGCCA 1 cut(s) 474
MlyI GAGTC 1 cut(s) 53
MmeI TCCRAC 1 cut(s) 354
MnlI CCTC 7 cut(s) 73, 77, 185, 247, 575, 802, 816
Mox20I TGGCCA 1 cut(s) 474
MscI TGGCCA 1 cut(s) 474
MseI TTAA 2 cut(s) 357, 581
Msp20I TGGCCA 1 cut(s) 474
MspI CCGG 1 cut(s) 548
MspR9I CCNGG 4 cut(s) 513, 548, 549, 722
MvaI CCWGG 2 cut(s) 513, 722
MvnI CGCG 2 cut(s) 406, 408
MwoI GCNNNNNNNGC 2 cut(s) 347, 459
NciI CCSGG 2 cut(s) 548, 549
NdeII GATC 2 cut(s) 243, 753
NlaIII CATG 6 cut(s) 187, 243, 473, 654, 719, 781
NmuCI GTSAC 4 cut(s) 115, 256, 479, 586
NspI RCATGY 1 cut(s) 781
NspV TTCGAA 1 cut(s) 294
PasI CCCWGGG 1 cut(s) 512
PciSI GCTCTTC 1 cut(s) 443
PfeI GAWTC 1 cut(s) 291
PflMI CCANNNNNTGG 2 cut(s) 518, 721
PkrI GCNGC 4 cut(s) 352, 420, 601, 689
PleI GAGTC 1 cut(s) 53
PmaCI CACGTG 1 cut(s) 249
PmlI CACGTG 1 cut(s) 249
PpsI GAGTC 1 cut(s) 53
Ppu21I YACGTR 1 cut(s) 249
Psp6I CCWGG 2 cut(s) 511, 720
PspCI CACGTG 1 cut(s) 249
PspFI CCCAGC 1 cut(s) 137
PspGI CCWGG 2 cut(s) 511, 720
PspPI GGNCC 2 cut(s) 172, 440
PstI CTGCAG 2 cut(s) 423, 689
RsaI GTAC 1 cut(s) 182
RsaNI GTAC 1 cut(s) 181
SapI GCTCTTC 1 cut(s) 443
SaqAI TTAA 2 cut(s) 357, 581
SatI GCNGC 4 cut(s) 351, 419, 600, 688
Sau3AI GATC 2 cut(s) 243, 753
Sau96I GGNCC 2 cut(s) 172, 440
SchI GAGTC 1 cut(s) 53
ScrFI CCNGG 4 cut(s) 513, 548, 549, 722
SduI GDGCHC 1 cut(s) 216
SfaNI GCATC 2 cut(s) 201, 533
SfcI CTRYAG 2 cut(s) 419, 685
SfuI TTCGAA 1 cut(s) 294
SmaI CCCGGG 1 cut(s) 549
SmlI CTYRAG 1 cut(s) 176
SmoI CTYRAG 1 cut(s) 176
Sse9I AATT 3 cut(s) 31, 163, 362
SsiI CCGC 4 cut(s) 391, 404, 408, 599
StyD4I CCNGG 4 cut(s) 511, 546, 547, 720
TaaI ACNGT 4 cut(s) 115, 479, 485, 646
TaiI ACGT 1 cut(s) 251
TaqI TCGA 1 cut(s) 294
TasI AATT 3 cut(s) 31, 163, 362
TatI WGTACW 1 cut(s) 180
TauI GCSGC 1 cut(s) 602
TfiI GAWTC 1 cut(s) 291
Tru1I TTAA 2 cut(s) 357, 581
Tru9I TTAA 2 cut(s) 357, 581
TscAI CASTG 3 cut(s) 118, 490, 609
TseFI GTSAC 4 cut(s) 115, 256, 479, 586
TseI GCWGC 3 cut(s) 350, 418, 687
Tsp45I GTSAC 4 cut(s) 115, 256, 479, 586
TspDTI ATGAA 2 cut(s) 322, 710
TspMI CCCGGG 1 cut(s) 547
TspRI CASTG 3 cut(s) 118, 490, 609
Van91I CCANNNNNTGG 2 cut(s) 518, 721
VneI GTGCAC 1 cut(s) 212
XagI CCTNNNNNAGG 1 cut(s) 67
XapI RAATTY 1 cut(s) 362
XceI RCATGY 1 cut(s) 781
XmaI CCCGGG 1 cut(s) 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.