MD03G1199400.v1.1

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0013358
Physical Location & Seq
Reverse (-)
27288491 .. 27290015
1525 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1199400.v1.1.491

Sequence Viewer

Length: 516 bp
ATGTTGCTGAAGCTCATAGACTCCTCCCTCAGGATTTTTGTAATTCCTCTCAGTATTGCAACCGTTTGGCTCACTGTCACCAACCACCAAGACAACCCCAGCTATGGCAACCTAAAGTTCTCCAATTTCATAGGCCTCAAGTACATGGTTTTCATCAGTACCATTTCTGGTGTGTATGCTTTTCTTGCAGCTTTGTCCTCATGGATCAGATGCTTTGCGACTAAAGCTTGGCTTTTCTTTGTCTCTGACCAGATTGTAGCTTATCTAATGGTCACATCTGGGGCAGCAGTGATGGAGATATTGTCTTTAGCTTACAAAGGGGACAGGACAGTCTCTTGGAGTGAAGCCTGCACTTCTTATGGGAAATTCTGCAGCAGAATGAAGGTGGCATTGGTTCTCCATGCCCTGGCTCTTTGCTGCTTTCTTGTATTGGCTGTGATCTCAGCTTATAGAATGTTTAGCATGTTTGACCCACCTTTTATTTCTGATAAGGAGGTGGAAGAAGTGAGGACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

172

Amino Acids

19.18

Weight (kDa)

8.61

Isoelectric Point (pI)

24.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 3 - 140 8.3e-30 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013358)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54980
fragaria_vesca FvH4_3g22910
malus_domestica MD03G1199400.v1.1 MD11G1216200.v1.1 MD11G1216300.v1.1
prunus_persica Prupe.4G215200_v2.0.a1
pyrus_communis pycom03g15100
rosa_chinensis RchiOBHm_Chr5g0040241
rosa_laevigata RLG00000033966
rosa_multiflora Rmu_sc0002908.1_g000012 Rmu_sc0010162.1_g000006
rosa_roxburghii Rroxscaffold_1G00041070
rosa_rugosa Rorug05G0184200
rosa_samantha Rh5AG271100 Rh5BG274400 Rh5CG307300 Rh5DG282600
rosa_wichuraiana Rw5G025350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 329
AccB7I CCANNNNNTGG 1 cut(s) 406
AclWI GGATC 1 cut(s) 212
AcsI RAATTY 1 cut(s) 365
AcuI CTGAAG 1 cut(s) 29
AfaI GTAC 2 cut(s) 143, 160
AfiI CCNNNNNNNGG 3 cut(s) 30, 104, 406
AjnI CCWGG 1 cut(s) 405
AjuI GAANNNNNNNTTGG 2 cut(s) 374, 406
AluBI AGCT 7 cut(s) 13, 102, 191, 227, 260, 311, 446
AluI AGCT 7 cut(s) 13, 102, 191, 227, 260, 311, 446
Alw26I GTCTC 2 cut(s) 247, 337
AlwI GGATC 1 cut(s) 212
AoxI GGCC 1 cut(s) 133
ApeKI GCWGC 4 cut(s) 188, 284, 372, 417
ApoI RAATTY 1 cut(s) 365
AsuHPI GGTGA 1 cut(s) 70
AxyI CCTNAGG 1 cut(s) 29
BbvI GCAGC 4 cut(s) 200, 296, 384, 404
BccI CCATC 1 cut(s) 286
BciT130I CCWGG 1 cut(s) 407
BcoDI GTCTC 2 cut(s) 247, 337
BfmI CTRYAG 1 cut(s) 370
BisI GCNGC 4 cut(s) 189, 285, 373, 418
BlsI GCNGC 4 cut(s) 190, 286, 374, 419
Bme1390I CCNGG 1 cut(s) 407
BmrFI CCNGG 1 cut(s) 407
BmsI GCATC 1 cut(s) 200
BpuEI CTTGAG 1 cut(s) 122
BsaJI CCNNGG 1 cut(s) 405
BsaXI ACNNNNNCTCC 2 cut(s) 287, 317
Bsc4I CCNNNNNNNGG 3 cut(s) 30, 104, 406
Bse21I CCTNAGG 1 cut(s) 29
BseBI CCWGG 1 cut(s) 407
BseDI CCNNGG 1 cut(s) 405
BseLI CCNNNNNNNGG 3 cut(s) 30, 104, 406
BseMII CTCAG 3 cut(s) 43, 64, 456
BseRI GAGGAG 1 cut(s) 13
BseXI GCAGC 4 cut(s) 200, 296, 384, 404
BseYI CCCAGC 1 cut(s) 98
BsgI GTGCAG 1 cut(s) 334
BshFI GGCC 1 cut(s) 135
BslFI GGGAC 1 cut(s) 335
BslI CCNNNNNNNGG 3 cut(s) 30, 104, 406
BsmAI GTCTC 2 cut(s) 247, 337
BsmFI GGGAC 1 cut(s) 335
BsnI GGCC 1 cut(s) 135
Bsp143I GATC 2 cut(s) 204, 438
BspANI GGCC 1 cut(s) 135
BspCNI CTCAG 3 cut(s) 42, 63, 455
BspMAI CTGCAG 1 cut(s) 374
BspPI GGATC 1 cut(s) 212
BssECI CCNNGG 1 cut(s) 405
BssMI GATC 2 cut(s) 204, 438
Bst2UI CCWGG 1 cut(s) 407
Bst4CI ACNGT 3 cut(s) 64, 76, 331
BstC8I GCNNGC 1 cut(s) 349
BstDEI CTNAG 4 cut(s) 29, 50, 442, 513
BstENI CCTNNNNNAGG 1 cut(s) 28
BstKTI GATC 2 cut(s) 207, 441
BstMAI GTCTC 2 cut(s) 247, 337
BstMBI GATC 2 cut(s) 204, 438
BstMWI GCNNNNNNNGC 2 cut(s) 185, 224
BstNI CCWGG 1 cut(s) 407
BstNSI RCATGY 1 cut(s) 466
BstSCI CCNGG 1 cut(s) 405
BstSFI CTRYAG 1 cut(s) 370
BstV1I GCAGC 4 cut(s) 200, 296, 384, 404
Bsu36I CCTNAGG 1 cut(s) 29
BsuRI GGCC 1 cut(s) 135
BtsI GCAGTG 1 cut(s) 294
BtsIMutI CAGTG 2 cut(s) 72, 294
Cac8I GCNNGC 1 cut(s) 349
Csp6I GTAC 2 cut(s) 142, 159
CviAII CATG 4 cut(s) 145, 201, 401, 463
CviQI GTAC 2 cut(s) 142, 159
DdeI CTNAG 4 cut(s) 29, 50, 442, 513
DpnI GATC 2 cut(s) 206, 440
DpnII GATC 2 cut(s) 204, 438
DrdI GACNNNNNNGTC 1 cut(s) 329
DseDI GACNNNNNNGTC 1 cut(s) 329
Eco147I AGGCCT 1 cut(s) 135
Eco57I CTGAAG 1 cut(s) 29
Eco81I CCTNAGG 1 cut(s) 29
EcoNI CCTNNNNNAGG 1 cut(s) 28
EcoRII CCWGG 1 cut(s) 405
FaeI CATG 4 cut(s) 148, 204, 404, 466
FalI AAGNNNNNCTT 2 cut(s) 216, 248
FaqI GGGAC 1 cut(s) 335
FatI CATG 4 cut(s) 144, 200, 400, 462
Fnu4HI GCNGC 4 cut(s) 189, 285, 373, 418
Fsp4HI GCNGC 4 cut(s) 189, 285, 373, 418
GluI GCNGC 4 cut(s) 189, 285, 373, 418
GsaI CCCAGC 1 cut(s) 102
HaeIII GGCC 1 cut(s) 135
Hin1II CATG 4 cut(s) 148, 204, 404, 466
HindIII AAGCTT 1 cut(s) 225
HinfI GANTC 1 cut(s) 20
HphI GGTGA 1 cut(s) 70
Hpy188I TCNGA 3 cut(s) 209, 247, 487
Hpy188III TCNNGA 1 cut(s) 31
HpyAV CCTTC 1 cut(s) 376
HpyCH4III ACNGT 3 cut(s) 64, 76, 331
HpyCH4V TGCA 4 cut(s) 59, 188, 351, 372
HpyF10VI GCNNNNNNNGC 2 cut(s) 185, 224
HpyF3I CTNAG 4 cut(s) 29, 50, 442, 513
Hsp92II CATG 4 cut(s) 148, 204, 404, 466
Kzo9I GATC 2 cut(s) 204, 438
LpnPI CCDG 9 cut(s) 16, 112, 153, 263, 264, 310, 361, 392, 419
Lsp1109I GCAGC 4 cut(s) 200, 296, 384, 404
LweI GCATC 1 cut(s) 200
MaeIII GTNAC 2 cut(s) 76, 271
MalI GATC 2 cut(s) 206, 440
MboI GATC 2 cut(s) 204, 438
MboII GAAGA 1 cut(s) 512
MluCI AATT 3 cut(s) 42, 124, 365
MlyI GAGTC 1 cut(s) 14
MnlI CCTC 7 cut(s) 34, 38, 57, 146, 208, 487, 501
MspR9I CCNGG 1 cut(s) 407
MvaI CCWGG 1 cut(s) 407
MwoI GCNNNNNNNGC 2 cut(s) 185, 224
NdeII GATC 2 cut(s) 204, 438
NlaIII CATG 4 cut(s) 148, 204, 404, 466
NmuCI GTSAC 2 cut(s) 76, 271
NspI RCATGY 1 cut(s) 466
PceI AGGCCT 1 cut(s) 135
PflMI CCANNNNNTGG 1 cut(s) 406
PkrI GCNGC 4 cut(s) 190, 286, 374, 419
PleI GAGTC 1 cut(s) 14
PpsI GAGTC 1 cut(s) 14
Psp6I CCWGG 1 cut(s) 405
PspFI CCCAGC 1 cut(s) 98
PspGI CCWGG 1 cut(s) 405
PstI CTGCAG 1 cut(s) 374
RsaI GTAC 2 cut(s) 143, 160
RsaNI GTAC 2 cut(s) 142, 159
SatI GCNGC 4 cut(s) 189, 285, 373, 418
Sau3AI GATC 2 cut(s) 204, 438
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 1 cut(s) 407
SfaNI GCATC 1 cut(s) 200
SfcI CTRYAG 1 cut(s) 370
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
Sse9I AATT 3 cut(s) 42, 124, 365
SseBI AGGCCT 1 cut(s) 135
StuI AGGCCT 1 cut(s) 135
StyD4I CCNGG 1 cut(s) 405
TaaI ACNGT 3 cut(s) 64, 76, 331
TasI AATT 3 cut(s) 42, 124, 365
TatI WGTACW 1 cut(s) 141
TscAI CASTG 2 cut(s) 79, 294
TseFI GTSAC 2 cut(s) 76, 271
TseI GCWGC 4 cut(s) 188, 284, 372, 417
Tsp45I GTSAC 2 cut(s) 76, 271
TspDTI ATGAA 3 cut(s) 118, 142, 395
TspRI CASTG 2 cut(s) 79, 294
Van91I CCANNNNNTGG 1 cut(s) 406
XagI CCTNNNNNAGG 1 cut(s) 28
XapI RAATTY 1 cut(s) 365
XceI RCATGY 1 cut(s) 466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.