FvH4_3g27971

Kelch

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0015578
Physical Location & Seq
Forward (+)
20995576 .. 20997703
2128 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g27971.t1

Sequence Viewer

Length: 1125 bp
ATGGGTTCCCTCCCATCTCCACCAAGACCTTCTTCTCCACCGCCATCATCGACTCATTCTCTGTCCAACTACAGAGTTTGTGCCACATTTTGTTTAAGAGAGCCCTCACCCAACTTAAACATCTCCAATTGGATCGAAATCTACGACCCATCAACCAACACGTGGACTCATGTGACCTCAATCCCTGGTCTCATTGAGAACCACATCTTGAAGGGTTTCTCCATGGTCTCGTTGGGCGACTCGCTTTATATAATTGGTGGTCGGTTATGTCACAAGGAGAGAGCTCACACTTCCGATGAGTGTAGCTCGGTCGTTGACATGGAAGTTCAAGTCTCATCATCGGTGTTGCGTTATAATGTTGCAAGTGATGAATGGTCAACTTGTGCACCACTTGGTGTAGCAAGGTGTGACTTTGCATGCACGGTTGCCAATAACAAAATATATGTGGCGGGTGGAAAGACCACATTGGCGTGTGCAAGAGGCATTTCATCCGCTGAGTTGTATGACCCCGAGCTTGACAAGTGGATTCATATGCCAAACATGAGCACATTGAGGTACAAGTGCGTTGGTGTCACATGGCAAAACAAAATCTACATTCTAGGTGGATTTGCAGAGCAAGTGAGAAAAAACTCAGACGTGCCTCCTATAATGGTGCGGAGCTCTGCTGAAGTTTATGACACACAGACAAAGAAATGGGAACTGATAATTGGGATGTGGCAATTGGATGTACCACCAAATCAAATCATTAACATGGGTGGGAGGCTTTTCAGTTCTGGAGATTGCTTCAAGCAATGGAAGGGTCACATTGAATCATATGATGGTGAACTCAACATATGGAATGAGGTTGATGGGTCTCATCTCCACAGTTTAAACTCACTCATTTCATCAGATAACGAAATGTGGGCACATAGCCAAAGGCGTTACCTAACTATGGCTCCCATCGGAGATCACTTGTACTTTTTGGCGATTCACGGATTAATGGACAATGATTTGTCGGGAACAATGTCGATTGTTCATAGATTCGACACTTCTGCCACGAGTAACGCTTGGACGAGCATGGAGCCGATGGAAGATGATAGGGAGAAAGAGCTCTGTGGACATGCTTGTGTTGTTCATCTTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_1

Protein Analysis

375

Amino Acids

41.71

Weight (kDa)

5.64

Isoelectric Point (pI)

50.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_ATRN-LZTR1 PF24981 39 - 152 2.2e-06 Attractin/LZTR1 beta-propeller
Kelch_KLHDC2_KLHL20_DRC7 PF24681 72 - 235 7.1e-10 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_FKB95 PF25210 74 - 293 1.2e-11 FKB95, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 109 - 209 2.2e-08 Attractin/LZTR1 beta-propeller
Beta-prop_Calicin PF13964 111 - 254 1.6e-09 Calicin, beta-propeller domain
NANM PF24996 119 - 207 7.6e-06 N-acetylneuraminate epimerase
Kelch_1 PF01344 134 - 182 1e-10 Kelch motif
Beta-prop_FBX42 PF13415 135 - 236 1.8e-07 FBX42, beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015578)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g27971
malus_domestica MD05G1322500.v1.1 MD10G1300400.v1.1
prunus_persica Prupe.4G040300_v2.0.a1
pyrus_communis pycom05g29670 pycom10g25320
rosa_chinensis RchiOBHm_Chr5g0007471
rosa_laevigata RLG00000031459
rosa_multiflora Rmu_ssc0000182.1_g000004
rosa_roxburghii Rroxscaffold_1G00068920
rosa_rugosa Rorug04G0427100
rosa_samantha Rh5AG055500 Rh5BG053100 Rh5CG062900 Rh5DG052100
rosa_wichuraiana Rw5G005460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 354
AccB7I CCANNNNNTGG 1 cut(s) 162
AciI CCGC 4 cut(s) 41, 449, 492, 655
AclWI GGATC 1 cut(s) 140
AcuI CTGAAG 1 cut(s) 687
AcvI CACGTG 1 cut(s) 162
AdeI CACNNNGTG 1 cut(s) 395
AfaI GTAC 3 cut(s) 557, 729, 956
AfiI CCNNNNNNNGG 2 cut(s) 162, 931
AflIII ACRYGT 1 cut(s) 159
AgsI TTSAA 4 cut(s) 211, 329, 787, 809
AjiI CACGTC 1 cut(s) 637
AjnI CCWGG 1 cut(s) 184
AjuI GAANNNNNNNTTGG 4 cut(s) 16, 48, 690, 722
AluBI AGCT 5 cut(s) 284, 306, 514, 660, 1090
AluI AGCT 5 cut(s) 284, 306, 514, 660, 1090
Alw21I GWGCWC 5 cut(s) 286, 388, 548, 662, 1092
Alw26I GTCTC 4 cut(s) 194, 232, 337, 858
Alw44I GTGCAC 1 cut(s) 384
AlwI GGATC 1 cut(s) 140
Ama87I CYCGRG 1 cut(s) 509
ApaLI GTGCAC 1 cut(s) 384
AseI ATTAAT 1 cut(s) 977
Asp700I GAANNNNTTC 1 cut(s) 215
AsuHPI GGTGA 2 cut(s) 99, 833
AvaI CYCGRG 1 cut(s) 509
BaeGI GKGCMC 2 cut(s) 388, 907
BanII GRGCYC 4 cut(s) 105, 286, 662, 1092
BauI CACGAG 1 cut(s) 1036
BbrPI CACGTG 1 cut(s) 162
Bbv12I GWGCWC 5 cut(s) 286, 388, 548, 662, 1092
BccI CCATC 7 cut(s) 22, 52, 157, 812, 842, 947, 1060
BcgI CGANNNNNNTGC 2 cut(s) 1013, 1047
BciT130I CCWGG 1 cut(s) 186
BcoDI GTCTC 4 cut(s) 194, 232, 337, 858
BfaI CTAG 1 cut(s) 599
BfmI CTRYAG 1 cut(s) 70
Bme1390I CCNGG 1 cut(s) 186
BmeT110I CYCGRG 1 cut(s) 509
BmgBI CACGTC 1 cut(s) 637
BmiI GGNNCC 3 cut(s) 7, 936, 1062
BmrFI CCNGG 1 cut(s) 186
BplI GAGNNNNNCTC 2 cut(s) 290, 322
BpmI CTGGAG 1 cut(s) 795
BsaAI YACGTR 1 cut(s) 162
BsaBI GATNNNNATC 1 cut(s) 137
BsaI GGTCTC 3 cut(s) 194, 232, 858
BsaJI CCNNGG 2 cut(s) 184, 222
BsaXI ACNNNNNCTCC 2 cut(s) 919, 949
Bsc4I CCNNNNNNNGG 2 cut(s) 162, 931
Bse3DI GCAATG 1 cut(s) 797
Bse8I GATNNNNATC 1 cut(s) 137
BseBI CCWGG 1 cut(s) 186
BseDI CCNNGG 2 cut(s) 184, 222
BseGI GGATG 3 cut(s) 488, 717, 730
BseJI GATNNNNATC 1 cut(s) 137
BseLI CCNNNNNNNGG 2 cut(s) 162, 931
BseMI GCAATG 1 cut(s) 797
BseMII CTCAG 2 cut(s) 486, 645
BseSI GKGCMC 2 cut(s) 388, 907
Bsh1285I CGRYCG 1 cut(s) 312
BsiEI CGRYCG 1 cut(s) 312
BsiHKAI GWGCWC 5 cut(s) 286, 388, 548, 662, 1092
BsiHKCI CYCGRG 1 cut(s) 509
BslI CCNNNNNNNGG 2 cut(s) 162, 931
BsmAI GTCTC 4 cut(s) 194, 232, 337, 858
Bso31I GGTCTC 3 cut(s) 194, 232, 858
BsoBI CYCGRG 1 cut(s) 509
Bsp1286I GDGCHC 7 cut(s) 105, 286, 388, 548, 662, 907, 1092
Bsp143I GATC 2 cut(s) 132, 946
Bsp19I CCATGG 1 cut(s) 222
BspACI CCGC 4 cut(s) 41, 449, 492, 655
BspCNI CTCAG 2 cut(s) 487, 644
BspLI GGNNCC 3 cut(s) 7, 936, 1062
BspPI GGATC 1 cut(s) 140
BspTNI GGTCTC 3 cut(s) 194, 232, 858
BsrDI GCAATG 1 cut(s) 797
BssECI CCNNGG 2 cut(s) 184, 222
BssMI GATC 2 cut(s) 132, 946
BssSI CACGAG 1 cut(s) 1036
BssT1I CCWWGG 1 cut(s) 222
Bst2BI CACGAG 1 cut(s) 1036
Bst2UI CCWGG 1 cut(s) 186
Bst4CI ACNGT 2 cut(s) 424, 866
BstBAI YACGTR 1 cut(s) 162
BstC8I GCNNGC 1 cut(s) 418
BstDEI CTNAG 3 cut(s) 495, 631, 1122
BstDSI CCRYGG 1 cut(s) 222
BstF5I GGATG 3 cut(s) 488, 717, 730
BstKTI GATC 2 cut(s) 135, 949
BstMAI GTCTC 4 cut(s) 194, 232, 337, 858
BstMBI GATC 2 cut(s) 132, 946
BstMCI CGRYCG 1 cut(s) 312
BstNI CCWGG 1 cut(s) 186
BstNSI RCATGY 2 cut(s) 420, 1103
BstSCI CCNGG 1 cut(s) 184
BstSFI CTRYAG 1 cut(s) 70
BstSLI GKGCMC 2 cut(s) 388, 907
BtgI CCRYGG 1 cut(s) 222
BtrI CACGTC 1 cut(s) 637
BtsCI GGATG 3 cut(s) 488, 717, 730
Cac8I GCNNGC 1 cut(s) 418
Csp6I GTAC 3 cut(s) 556, 728, 955
CviAII CATG 9 cut(s) 170, 223, 319, 417, 541, 576, 751, 1057, 1100
CviQI GTAC 3 cut(s) 556, 728, 955
DdeI CTNAG 3 cut(s) 495, 631, 1122
DpnI GATC 2 cut(s) 134, 948
DpnII GATC 2 cut(s) 132, 946
DraI TTTAAA 1 cut(s) 870
DraIII CACNNNGTG 1 cut(s) 395
Ecl136II GAGCTC 3 cut(s) 284, 660, 1090
Eco130I CCWWGG 1 cut(s) 222
Eco24I GRGCYC 4 cut(s) 105, 286, 662, 1092
Eco31I GGTCTC 3 cut(s) 194, 232, 858
Eco53kI GAGCTC 3 cut(s) 284, 660, 1090
Eco57I CTGAAG 1 cut(s) 687
Eco72I CACGTG 1 cut(s) 162
Eco88I CYCGRG 1 cut(s) 509
EcoICRI GAGCTC 3 cut(s) 284, 660, 1090
EcoRII CCWGG 1 cut(s) 184
EcoT14I CCWWGG 1 cut(s) 222
EcoT38I GRGCYC 4 cut(s) 105, 286, 662, 1092
ErhI CCWWGG 1 cut(s) 222
FaeI CATG 9 cut(s) 173, 226, 322, 420, 544, 579, 754, 1060, 1103
FalI AAGNNNNNCTT 2 cut(s) 16, 48
FatI CATG 9 cut(s) 169, 222, 318, 416, 540, 575, 750, 1056, 1099
FauI CCCGC 1 cut(s) 442
FauNDI CATATG 3 cut(s) 531, 814, 833
FokI GGATG 3 cut(s) 475, 724, 737
FriOI GRGCYC 4 cut(s) 105, 286, 662, 1092
FspBI CTAG 1 cut(s) 599
GsuI CTGGAG 1 cut(s) 795
Hin1II CATG 9 cut(s) 173, 226, 322, 420, 544, 579, 754, 1060, 1103
HincII GTYRAC 2 cut(s) 316, 378
HindII GTYRAC 2 cut(s) 316, 378
HinfI GANTC 7 cut(s) 52, 166, 239, 526, 809, 967, 1020
HphI GGTGA 2 cut(s) 99, 833
Hpy166II GTNNAC 6 cut(s) 165, 316, 378, 386, 824, 1097
Hpy188I TCNGA 4 cut(s) 295, 634, 889, 944
Hpy188III TCNNGA 3 cut(s) 208, 774, 996
Hpy8I GTNNAC 6 cut(s) 165, 316, 378, 386, 824, 1097
HpyAV CCTTC 3 cut(s) 39, 205, 790
HpyCH4III ACNGT 2 cut(s) 424, 866
HpyCH4IV ACGT 2 cut(s) 161, 636
HpyCH4V TGCA 6 cut(s) 362, 386, 416, 420, 476, 611
HpyF3I CTNAG 3 cut(s) 495, 631, 1122
HpySE526I ACGT 2 cut(s) 161, 636
Hsp92II CATG 9 cut(s) 173, 226, 322, 420, 544, 579, 754, 1060, 1103
Kzo9I GATC 2 cut(s) 132, 946
LmnI GCTCC 3 cut(s) 657, 940, 1060
LpnPI CCDG 3 cut(s) 171, 198, 759
MaeI CTAG 1 cut(s) 599
MaeII ACGT 2 cut(s) 161, 636
MaeIII GTNAC 7 cut(s) 172, 269, 407, 571, 800, 920, 1040
MalI GATC 2 cut(s) 134, 948
MboI GATC 2 cut(s) 132, 946
MboII GAAGA 2 cut(s) 24, 1082
MfeI CAATTG 2 cut(s) 127, 719
MhlI GDGCHC 7 cut(s) 105, 286, 388, 548, 662, 907, 1092
MluCI AATT 4 cut(s) 127, 252, 705, 719
MlyI GAGTC 3 cut(s) 46, 160, 233
MmeI TCCRAC 1 cut(s) 90
MnlI CCTC 8 cut(s) 20, 115, 187, 473, 546, 651, 753, 835
MroXI GAANNNNTTC 1 cut(s) 215
MseI TTAA 5 cut(s) 95, 116, 747, 869, 977
MslI CAYNNNNRTG 3 cut(s) 469, 749, 1104
MspA1I CMGCKG 1 cut(s) 494
MspR9I CCNGG 1 cut(s) 186
MssI GTTTAAAC 1 cut(s) 870
MunI CAATTG 2 cut(s) 127, 719
MvaI CCWGG 1 cut(s) 186
NcoI CCATGG 1 cut(s) 222
NdeI CATATG 3 cut(s) 531, 814, 833
NdeII GATC 2 cut(s) 132, 946
NlaIII CATG 9 cut(s) 173, 226, 322, 420, 544, 579, 754, 1060, 1103
NlaIV GGNNCC 3 cut(s) 7, 936, 1062
NmuCI GTSAC 5 cut(s) 172, 269, 407, 571, 800
NspI RCATGY 2 cut(s) 420, 1103
PaeI GCATGC 1 cut(s) 420
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PdmI GAANNNNTTC 1 cut(s) 215
PfeI GAWTC 4 cut(s) 526, 809, 967, 1020
PflMI CCANNNNNTGG 1 cut(s) 162
PleI GAGTC 3 cut(s) 46, 160, 233
PmaCI CACGTG 1 cut(s) 162
PmeI GTTTAAAC 1 cut(s) 870
PmlI CACGTG 1 cut(s) 162
PpsI GAGTC 3 cut(s) 46, 160, 233
Ppu21I YACGTR 1 cut(s) 162
PshBI ATTAAT 1 cut(s) 977
PsiI TTATAA 1 cut(s) 354
Psp124BI GAGCTC 3 cut(s) 286, 662, 1092
Psp6I CCWGG 1 cut(s) 184
PspCI CACGTG 1 cut(s) 162
PspGI CCWGG 1 cut(s) 184
PspN4I GGNNCC 3 cut(s) 7, 936, 1062
RsaI GTAC 3 cut(s) 557, 729, 956
RsaNI GTAC 3 cut(s) 556, 728, 955
RseI CAYNNNNRTG 3 cut(s) 469, 749, 1104
SacI GAGCTC 3 cut(s) 286, 662, 1092
SaqAI TTAA 5 cut(s) 95, 116, 747, 869, 977
Sau3AI GATC 2 cut(s) 132, 946
SchI GAGTC 3 cut(s) 46, 160, 233
ScrFI CCNGG 1 cut(s) 186
SduI GDGCHC 7 cut(s) 105, 286, 388, 548, 662, 907, 1092
SfcI CTRYAG 1 cut(s) 70
SmiMI CAYNNNNRTG 3 cut(s) 469, 749, 1104
SphI GCATGC 1 cut(s) 420
Sse9I AATT 4 cut(s) 127, 252, 705, 719
SsiI CCGC 4 cut(s) 41, 449, 492, 655
SspMI CTAG 1 cut(s) 599
SstI GAGCTC 3 cut(s) 286, 662, 1092
StyD4I CCNGG 1 cut(s) 184
StyI CCWWGG 1 cut(s) 222
TaaI ACNGT 2 cut(s) 424, 866
TaiI ACGT 2 cut(s) 164, 639
TaqI TCGA 4 cut(s) 50, 135, 1007, 1023
TaqII GACCGA 1 cut(s) 298
TasI AATT 4 cut(s) 127, 252, 705, 719
TatI WGTACW 1 cut(s) 954
TfiI GAWTC 4 cut(s) 526, 809, 967, 1020
Tru1I TTAA 5 cut(s) 95, 116, 747, 869, 977
Tru9I TTAA 5 cut(s) 95, 116, 747, 869, 977
TseFI GTSAC 5 cut(s) 172, 269, 407, 571, 800
Tsp45I GTSAC 5 cut(s) 172, 269, 407, 571, 800
TspDTI ATGAA 6 cut(s) 384, 477, 518, 873, 1004, 1103
TspGWI ACGGA 1 cut(s) 987
Van91I CCANNNNNTGG 1 cut(s) 162
VneI GTGCAC 1 cut(s) 384
VspI ATTAAT 1 cut(s) 977
XceI RCATGY 2 cut(s) 420, 1103
XcmI CCANNNNNNNNNTGG 1 cut(s) 229
XmnI GAANNNNTTC 1 cut(s) 215
XspI CTAG 1 cut(s) 599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.