Prupe.4G040300_v2.0.a1

Kelch

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0015578
Physical Location & Seq
Reverse (-)
1894803 .. 1895933
1131 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G040300.1

Sequence Viewer

Length: 1131 bp
ATGGGTTCTCTCCCATCCCCACCAAGACCCTCAACTCCACCACCTTCCTCACCAAACAACTCTCTATCCAGTTACAGGGTTTGCGCCACATTTTGCTTCAGAGAATCAGCGCCAAACTTGAACATCTCTAACTGGATAGAGTTCTACGACCCATCAACCAACACATGGACACATGCTAGCTCGATCCCTGGCCTCACTGAGAACCATATCTTAAAGGGTTTTGCCATGGTGTCTTTAGGTGACTCGGTTTACATAATTGGTGGTAGGGTTTGCCGCAAGGAGAGAGCTCACACATCAGATGAGTGCAGTGAGCTTGTTGACGTGGATATTGAAGTCTCTTCATCTGTGTTGCGTTACAATGTTGGGTCTGACCAATGGTCAACGTGTGCACCACTTGGCATCTCGAGGTGTGACTTTGCCTGCACGATTTTCGAGAACAAGATCTATGTGGCCGGGGGTAAGTCCACGCTAGCATGCGCAAGGGGGATTCCATTAGCTGAGGTGTACGATCCCGAGCTCAACGAGTGGACCCCGCTGCCTAACATGAGCACATTGAGGTACAATTGCGTGGGCGTGACATGGCTAGGTAAAATCTACGTGGTGGGAGGTTTCGCAGAGAGGGCTGACTCGGAGCTGCCTCCAATAATGGTGCGCAGCTGTGCTGAGGTGTACGACACGCAAACAAGGATGTGGGACCTCATAGTGGGGATGTGGCAATTGGATGTGCCACCAAATCAAATTGTGGAAGTAGATGGGAGACTTTTTAGCTCTGGTGATTGCTACAAGCAATGGAAAGGTCACATTGAGTCATATGATGGGAAGCTCAACATATGGAATGAAGTTGAAGGGTCCCAAATACAAAGTCTGAATTCCCTGATATCTGCATTGGGCACGCAAGATGATCACAACTGGGCACTTAGCCAGAGGCGTTACCTAACAATGGCACCTATTGGCGTTAACTTGTACTTCCTGGCAGGGTATGGCATATACAGAGAATTGTCAAGAACAATGTCGATCGTTCATAAATTTGATATCTCAGCAACCAGTGATGCATGGACAAGCATGGAGCCGATGGAGGAGGATGGAGAGAAAGAACTCTGTGGGCATTGCTGTGTTGTTCAACTCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_1

Protein Analysis

377

Amino Acids

41.81

Weight (kDa)

4.86

Isoelectric Point (pI)

47.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015578)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g27971
malus_domestica MD05G1322500.v1.1 MD10G1300400.v1.1
prunus_persica Prupe.4G040300_v2.0.a1
pyrus_communis pycom05g29670 pycom10g25320
rosa_chinensis RchiOBHm_Chr5g0007471
rosa_laevigata RLG00000031459
rosa_multiflora Rmu_ssc0000182.1_g000004
rosa_roxburghii Rroxscaffold_1G00068920
rosa_rugosa Rorug04G0427100
rosa_samantha Rh5AG055500 Rh5BG053100 Rh5CG062900 Rh5DG052100
rosa_wichuraiana Rw5G005460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 478, 653
AccB1I GGYRCC 1 cut(s) 943
AccB7I CCANNNNNTGG 1 cut(s) 165
AciI CCGC 2 cut(s) 274, 533
AclWI GGATC 2 cut(s) 178, 503
AcoI YGGCCR 1 cut(s) 450
AcsI RAATTY 2 cut(s) 868, 1025
AcuI CTGAAG 1 cut(s) 82
AfaI GTAC 4 cut(s) 506, 560, 671, 965
AfiI CCNNNNNNNGG 4 cut(s) 75, 165, 703, 940
AflIII ACRYGT 1 cut(s) 383
AgsI TTSAA 4 cut(s) 121, 332, 845, 1121
AhdI GACNNNNNGTC 1 cut(s) 376
AjiI CACGTC 1 cut(s) 322
AjnI CCWGG 2 cut(s) 187, 969
AluBI AGCT 9 cut(s) 180, 287, 313, 497, 517, 634, 657, 768, 823
AluI AGCT 9 cut(s) 180, 287, 313, 497, 517, 634, 657, 768, 823
Alw21I GWGCWC 4 cut(s) 289, 391, 519, 551
Alw26I GTCTC 2 cut(s) 340, 751
Alw44I GTGCAC 1 cut(s) 387
AlwI GGATC 2 cut(s) 178, 503
Ama87I CYCGRG 2 cut(s) 403, 512
AoxI GGCC 2 cut(s) 190, 450
ApaLI GTGCAC 1 cut(s) 387
ApeKI GCWGC 3 cut(s) 535, 634, 654
ApoI RAATTY 2 cut(s) 868, 1025
ArsI GACNNNNNNTTYG 2 cut(s) 847, 879
AspLEI GCGC 4 cut(s) 86, 112, 479, 654
AspS9I GGNCC 3 cut(s) 528, 694, 849
AsuC2I CCSGG 1 cut(s) 454
AsuHPI GGTGA 3 cut(s) 42, 251, 785
AsuNHI GCTAGC 2 cut(s) 176, 469
AvaI CYCGRG 2 cut(s) 403, 512
AvaII GGWCC 3 cut(s) 528, 694, 849
BaeGI GKGCMC 3 cut(s) 391, 893, 916
BanI GGYRCC 1 cut(s) 943
BanII GRGCYC 2 cut(s) 289, 519
Bbv12I GWGCWC 4 cut(s) 289, 391, 519, 551
BbvCI CCTCAGC 2 cut(s) 498, 663
BbvI GCAGC 3 cut(s) 522, 621, 666
BccI CCATC 6 cut(s) 22, 160, 746, 809, 1066, 1076
BcgI CGANNNNNNTGC 2 cut(s) 412, 446
BciT130I CCWGG 2 cut(s) 189, 971
BclI TGATCA 1 cut(s) 901
BcnI CCSGG 1 cut(s) 454
BcoDI GTCTC 2 cut(s) 340, 751
BfaI CTAG 3 cut(s) 177, 470, 584
BfoI RGCGCY 1 cut(s) 113
BglII AGATCT 1 cut(s) 441
BisI GCNGC 4 cut(s) 274, 536, 635, 655
BlsI GCNGC 4 cut(s) 275, 537, 636, 656
Bme1390I CCNGG 3 cut(s) 189, 454, 971
Bme18I GGWCC 3 cut(s) 528, 694, 849
BmeRI GACNNNNNGTC 1 cut(s) 376
BmeT110I CYCGRG 2 cut(s) 403, 512
BmgBI CACGTC 1 cut(s) 322
BmgT120I GGNCC 3 cut(s) 528, 694, 849
BmiI GGNNCC 6 cut(s) 530, 695, 850, 851, 945, 1068
BmrFI CCNGG 3 cut(s) 189, 454, 971
BmrI ACTGGG 1 cut(s) 919
BmsI GCATC 2 cut(s) 408, 1039
BmtI GCTAGC 2 cut(s) 180, 473
BmuI ACTGGG 1 cut(s) 919
Bpu10I CCTNAGC 2 cut(s) 498, 663
BpuMI CCSGG 1 cut(s) 454
BsaAI YACGTR 1 cut(s) 598
BsaJI CCNNGG 3 cut(s) 187, 225, 453
Bsc4I CCNNNNNNNGG 4 cut(s) 75, 165, 703, 940
Bse1I ACTGG 4 cut(s) 69, 137, 914, 1044
Bse3DI GCAATG 2 cut(s) 794, 1105
BseBI CCWGG 2 cut(s) 189, 971
BseDI CCNNGG 3 cut(s) 187, 225, 453
BseGI GGATG 5 cut(s) 14, 693, 714, 727, 1087
BseLI CCNNNNNNNGG 4 cut(s) 75, 165, 703, 940
BseMI GCAATG 2 cut(s) 794, 1105
BseMII CTCAG 4 cut(s) 189, 489, 654, 1050
BseNI ACTGG 4 cut(s) 69, 137, 914, 1044
BseRI GAGGAG 1 cut(s) 1091
BseSI GKGCMC 3 cut(s) 391, 893, 916
BseXI GCAGC 3 cut(s) 522, 621, 666
BsgI GTGCAG 2 cut(s) 325, 406
Bsh1285I CGRYCG 1 cut(s) 1017
BshFI GGCC 2 cut(s) 192, 452
BshNI GGYRCC 1 cut(s) 943
BsiEI CGRYCG 1 cut(s) 1017
BsiHKAI GWGCWC 4 cut(s) 289, 391, 519, 551
BsiHKCI CYCGRG 2 cut(s) 403, 512
BsiSI CCGG 1 cut(s) 453
BslFI GGGAC 2 cut(s) 707, 835
BslI CCNNNNNNNGG 4 cut(s) 75, 165, 703, 940
BsmAI GTCTC 2 cut(s) 340, 751
BsmFI GGGAC 2 cut(s) 707, 835
BsnI GGCC 2 cut(s) 192, 452
BsoBI CYCGRG 2 cut(s) 403, 512
Bsp1286I GDGCHC 6 cut(s) 289, 391, 519, 551, 893, 916
Bsp143I GATC 5 cut(s) 183, 441, 508, 901, 1014
Bsp19I CCATGG 1 cut(s) 225
BspACI CCGC 2 cut(s) 274, 533
BspANI GGCC 2 cut(s) 192, 452
BspCNI CTCAG 4 cut(s) 190, 490, 655, 1049
BspLI GGNNCC 6 cut(s) 530, 695, 850, 851, 945, 1068
BspOI GCTAGC 2 cut(s) 180, 473
BspPI GGATC 2 cut(s) 178, 503
BspT107I GGYRCC 1 cut(s) 943
BsrDI GCAATG 2 cut(s) 794, 1105
BsrI ACTGG 4 cut(s) 69, 137, 914, 1044
BssECI CCNNGG 3 cut(s) 187, 225, 453
BssMI GATC 5 cut(s) 183, 441, 508, 901, 1014
BssT1I CCWWGG 1 cut(s) 225
Bst2UI CCWGG 2 cut(s) 189, 971
Bst6I CTCTTC 1 cut(s) 343
BstBAI YACGTR 1 cut(s) 598
BstC8I GCNNGC 5 cut(s) 178, 421, 471, 475, 893
BstDEI CTNAG 5 cut(s) 198, 498, 663, 917, 1036
BstDSI CCRYGG 1 cut(s) 225
BstF5I GGATG 5 cut(s) 14, 693, 714, 727, 1087
BstH2I RGCGCY 1 cut(s) 113
BstHHI GCGC 4 cut(s) 86, 112, 479, 654
BstKTI GATC 5 cut(s) 186, 444, 511, 904, 1017
BstMAI GTCTC 2 cut(s) 340, 751
BstMBI GATC 5 cut(s) 183, 441, 508, 901, 1014
BstMCI CGRYCG 1 cut(s) 1017
BstMWI GCNNNNNNNGC 1 cut(s) 620
BstNI CCWGG 2 cut(s) 189, 971
BstNSI RCATGY 2 cut(s) 176, 477
BstSCI CCNGG 3 cut(s) 187, 452, 969
BstSLI GKGCMC 3 cut(s) 391, 893, 916
BstV1I GCAGC 3 cut(s) 522, 621, 666
BstX2I RGATCY 1 cut(s) 441
BstYI RGATCY 1 cut(s) 441
BsuRI GGCC 2 cut(s) 192, 452
BtgI CCRYGG 1 cut(s) 225
BtrI CACGTC 1 cut(s) 322
BtsCI GGATG 5 cut(s) 14, 693, 714, 727, 1087
BtsI GCAGTG 1 cut(s) 313
BtsIMutI CAGTG 3 cut(s) 195, 313, 1051
Cac8I GCNNGC 5 cut(s) 178, 421, 471, 475, 893
CfoI GCGC 4 cut(s) 86, 112, 479, 654
Cfr13I GGNCC 3 cut(s) 528, 694, 849
Csp6I GTAC 4 cut(s) 505, 559, 670, 964
CviAII CATG 9 cut(s) 165, 173, 226, 474, 544, 579, 1053, 1063, 1128
CviQI GTAC 4 cut(s) 505, 559, 670, 964
DdeI CTNAG 5 cut(s) 198, 498, 663, 917, 1036
DpnI GATC 5 cut(s) 185, 443, 510, 903, 1016
DpnII GATC 5 cut(s) 183, 441, 508, 901, 1014
DriI GACNNNNNGTC 1 cut(s) 376
EaeI YGGCCR 1 cut(s) 450
Eam1104I CTCTTC 1 cut(s) 343
Eam1105I GACNNNNNGTC 1 cut(s) 376
EarI CTCTTC 1 cut(s) 343
Ecl136II GAGCTC 2 cut(s) 287, 517
Eco130I CCWWGG 1 cut(s) 225
Eco24I GRGCYC 2 cut(s) 289, 519
Eco32I GATATC 2 cut(s) 879, 1033
Eco47I GGWCC 3 cut(s) 528, 694, 849
Eco53kI GAGCTC 2 cut(s) 287, 517
Eco57I CTGAAG 1 cut(s) 82
Eco88I CYCGRG 2 cut(s) 403, 512
EcoICRI GAGCTC 2 cut(s) 287, 517
EcoO109I RGGNCCY 2 cut(s) 694, 849
EcoRI GAATTC 1 cut(s) 868
EcoRII CCWGG 2 cut(s) 187, 969
EcoRV GATATC 2 cut(s) 879, 1033
EcoT14I CCWWGG 1 cut(s) 225
EcoT22I ATGCAT 1 cut(s) 1054
EcoT38I GRGCYC 2 cut(s) 289, 519
ErhI CCWWGG 1 cut(s) 225
FaeI CATG 9 cut(s) 168, 176, 229, 477, 547, 582, 1056, 1066, 1131
FaqI GGGAC 2 cut(s) 707, 835
FatI CATG 9 cut(s) 164, 172, 225, 473, 543, 578, 1052, 1062, 1127
FauI CCCGC 1 cut(s) 540
FauNDI CATATG 2 cut(s) 811, 830
FbaI TGATCA 1 cut(s) 901
Fnu4HI GCNGC 4 cut(s) 274, 536, 635, 655
FokI GGATG 4 cut(s) 700, 721, 734, 1094
FriOI GRGCYC 2 cut(s) 289, 519
Fsp4HI GCNGC 4 cut(s) 274, 536, 635, 655
FspBI CTAG 3 cut(s) 177, 470, 584
FspI TGCGCA 2 cut(s) 478, 653
GlaI GCGC 4 cut(s) 85, 111, 478, 653
GluI GCNGC 4 cut(s) 274, 536, 635, 655
HaeII RGCGCY 1 cut(s) 113
HaeIII GGCC 2 cut(s) 192, 452
HapII CCGG 1 cut(s) 453
HhaI GCGC 4 cut(s) 86, 112, 479, 654
Hin1II CATG 9 cut(s) 168, 176, 229, 477, 547, 582, 1056, 1066, 1131
Hin6I GCGC 4 cut(s) 84, 110, 477, 652
HinP1I GCGC 4 cut(s) 84, 110, 477, 652
HincII GTYRAC 3 cut(s) 319, 381, 958
HindII GTYRAC 3 cut(s) 319, 381, 958
HinfI GANTC 5 cut(s) 104, 242, 487, 626, 806
HpaI GTTAAC 1 cut(s) 958
HpaII CCGG 1 cut(s) 453
HphI GGTGA 3 cut(s) 42, 251, 785
Hpy166II GTNNAC 9 cut(s) 250, 319, 381, 389, 465, 505, 528, 670, 958
Hpy188I TCNGA 5 cut(s) 101, 298, 370, 631, 867
Hpy188III TCNNGA 4 cut(s) 403, 433, 512, 1002
Hpy8I GTNNAC 9 cut(s) 250, 319, 381, 389, 465, 505, 528, 670, 958
HpyAV CCTTC 2 cut(s) 54, 839
HpyCH4IV ACGT 3 cut(s) 321, 383, 597
HpyCH4V TGCA 5 cut(s) 306, 389, 423, 884, 1052
HpyF10VI GCNNNNNNNGC 1 cut(s) 620
HpyF3I CTNAG 5 cut(s) 198, 498, 663, 917, 1036
HpySE526I ACGT 3 cut(s) 321, 383, 597
Hsp92II CATG 9 cut(s) 168, 176, 229, 477, 547, 582, 1056, 1066, 1131
HspAI GCGC 4 cut(s) 84, 110, 477, 652
KflI GGGWCCC 1 cut(s) 849
Ksp22I TGATCA 1 cut(s) 901
KspAI GTTAAC 1 cut(s) 958
Kzo9I GATC 5 cut(s) 183, 441, 508, 901, 1014
LmnI GCTCC 2 cut(s) 631, 1066
Lsp1109I GCAGC 3 cut(s) 522, 621, 666
LweI GCATC 2 cut(s) 408, 1039
MaeI CTAG 3 cut(s) 177, 470, 584
MaeII ACGT 3 cut(s) 321, 383, 597
MaeIII GTNAC 7 cut(s) 71, 239, 353, 410, 574, 797, 929
MalI GATC 5 cut(s) 185, 443, 510, 903, 1016
MboI GATC 5 cut(s) 183, 441, 508, 901, 1014
MboII GAAGA 1 cut(s) 330
MfeI CAATTG 2 cut(s) 562, 716
MflI RGATCY 1 cut(s) 441
MhlI GDGCHC 6 cut(s) 289, 391, 519, 551, 893, 916
MluCI AATT 7 cut(s) 255, 562, 716, 738, 868, 995, 1025
MlyI GAGTC 3 cut(s) 236, 620, 815
Mph1103I ATGCAT 1 cut(s) 1054
MseI TTAA 2 cut(s) 212, 957
MslI CAYNNNNRTG 1 cut(s) 1110
MspA1I CMGCKG 2 cut(s) 535, 657
MspI CCGG 1 cut(s) 453
MspR9I CCNGG 3 cut(s) 189, 454, 971
MunI CAATTG 2 cut(s) 562, 716
MvaI CCWGG 2 cut(s) 189, 971
MwoI GCNNNNNNNGC 1 cut(s) 620
NciI CCSGG 1 cut(s) 454
NcoI CCATGG 1 cut(s) 225
NdeI CATATG 2 cut(s) 811, 830
NdeII GATC 5 cut(s) 183, 441, 508, 901, 1014
NheI GCTAGC 2 cut(s) 176, 469
NlaIII CATG 9 cut(s) 168, 176, 229, 477, 547, 582, 1056, 1066, 1131
NlaIV GGNNCC 6 cut(s) 530, 695, 850, 851, 945, 1068
NmuCI GTSAC 4 cut(s) 239, 410, 574, 797
NsbI TGCGCA 2 cut(s) 478, 653
NsiI ATGCAT 1 cut(s) 1054
NspI RCATGY 2 cut(s) 176, 477
PaeI GCATGC 1 cut(s) 477
PaeR7I CTCGAG 1 cut(s) 403
PfeI GAWTC 2 cut(s) 104, 487
PflMI CCANNNNNTGG 1 cut(s) 165
PkrI GCNGC 4 cut(s) 275, 537, 636, 656
Ple19I CGATCG 1 cut(s) 1017
PleI GAGTC 3 cut(s) 236, 620, 814
PpsI GAGTC 3 cut(s) 236, 620, 814
Ppu21I YACGTR 1 cut(s) 598
PpuMI RGGWCCY 2 cut(s) 694, 849
Psp124BI GAGCTC 2 cut(s) 289, 519
Psp5II RGGWCCY 2 cut(s) 694, 849
Psp6I CCWGG 2 cut(s) 187, 969
PspGI CCWGG 2 cut(s) 187, 969
PspN4I GGNNCC 6 cut(s) 530, 695, 850, 851, 945, 1068
PspPI GGNCC 3 cut(s) 528, 694, 849
PspPPI RGGWCCY 2 cut(s) 694, 849
PsuI RGATCY 1 cut(s) 441
PvuI CGATCG 1 cut(s) 1017
PvuII CAGCTG 1 cut(s) 657
RsaI GTAC 4 cut(s) 506, 560, 671, 965
RsaNI GTAC 4 cut(s) 505, 559, 670, 964
RseI CAYNNNNRTG 1 cut(s) 1110
SacI GAGCTC 2 cut(s) 289, 519
SaqAI TTAA 2 cut(s) 212, 957
SatI GCNGC 4 cut(s) 274, 536, 635, 655
Sau3AI GATC 5 cut(s) 183, 441, 508, 901, 1014
Sau96I GGNCC 3 cut(s) 528, 694, 849
SchI GAGTC 3 cut(s) 236, 620, 815
ScrFI CCNGG 3 cut(s) 189, 454, 971
SduI GDGCHC 6 cut(s) 289, 391, 519, 551, 893, 916
SfaNI GCATC 2 cut(s) 408, 1039
Sfr274I CTCGAG 1 cut(s) 403
SinI GGWCC 3 cut(s) 528, 694, 849
SlaI CTCGAG 1 cut(s) 403
SmiMI CAYNNNNRTG 1 cut(s) 1110
SmlI CTYRAG 1 cut(s) 403
SmoI CTYRAG 1 cut(s) 403
SphI GCATGC 1 cut(s) 477
Sse9I AATT 7 cut(s) 255, 562, 716, 738, 868, 995, 1025
SsiI CCGC 2 cut(s) 274, 533
SspMI CTAG 3 cut(s) 177, 470, 584
SstI GAGCTC 2 cut(s) 289, 519
StyD4I CCNGG 3 cut(s) 187, 452, 969
StyI CCWWGG 1 cut(s) 225
TaiI ACGT 3 cut(s) 324, 386, 600
TaqI TCGA 4 cut(s) 182, 404, 432, 1013
TasI AATT 7 cut(s) 255, 562, 716, 738, 868, 995, 1025
TatI WGTACW 1 cut(s) 963
TauI GCSGC 1 cut(s) 276
TfiI GAWTC 2 cut(s) 104, 487
Tru1I TTAA 2 cut(s) 212, 957
Tru9I TTAA 2 cut(s) 212, 957
TscAI CASTG 3 cut(s) 202, 313, 1051
TseFI GTSAC 4 cut(s) 239, 410, 574, 797
TseI GCWGC 3 cut(s) 535, 634, 654
Tsp45I GTSAC 4 cut(s) 239, 410, 574, 797
TspDTI ATGAA 3 cut(s) 330, 852, 1010
TspRI CASTG 3 cut(s) 202, 313, 1051
Van91I CCANNNNNTGG 1 cut(s) 165
VneI GTGCAC 1 cut(s) 387
VpaK11BI GGWCC 3 cut(s) 528, 694, 849
XapI RAATTY 2 cut(s) 868, 1025
XceI RCATGY 2 cut(s) 176, 477
XhoI CTCGAG 1 cut(s) 403
XspI CTAG 3 cut(s) 177, 470, 584
Zsp2I ATGCAT 1 cut(s) 1054
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.