Rorug04G0427100

Kelch

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0015578
Physical Location & Seq
Forward (+)
58568815 .. 58569483
669 bp
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UTR
Exon/CDS
Intron
Rorug04G0427100.1

Sequence Viewer

Length: 456 bp
ATGAAGCAAGGTGACTGGATCTGCCCCAAAGAGACTGGAAAAACTAAGGGAGGCTCAAGATCATCTTCCATTGAAGAAGGGGGACTGGTTGTGAAAAGACAAGGTAGAAATGGTCAAAACAGAGGTGCAAGCAGGTGGAGATTTGTGGTTGAAGAGAATGATGATCCTCGTCACTCAAACTCATTAGATAAGACTTCCAGGTTTGCAGACTTCCCAATTGTACGAGGTAAGACCGAACTGTCTCAGAATCCACAAAAGAGGGACAGATGGAAGTTGAAGATGTTAGAGAAGAGCAAAGGAGGCGCAACGGATATGGCAAGTGATGATGAACTTAGGTCTACCAGTACTGAAAGAAGAGTATTCTTTTCTGATTCTGCAGATGATGAAGAGATGGCTGAGTGGTTTGGGGATAGAAAGCTAGAAACTTGTGGCTTCCATCCAGGCCTAAGTGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_1

Protein Analysis

151

Amino Acids

17.07

Weight (kDa)

8.58

Isoelectric Point (pI)

55.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015578)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g27971
malus_domestica MD05G1322500.v1.1 MD10G1300400.v1.1
prunus_persica Prupe.4G040300_v2.0.a1
pyrus_communis pycom05g29670 pycom10g25320
rosa_chinensis RchiOBHm_Chr5g0007471
rosa_laevigata RLG00000031459
rosa_multiflora Rmu_ssc0000182.1_g000004
rosa_roxburghii Rroxscaffold_1G00068920
rosa_rugosa Rorug04G0427100
rosa_samantha Rh5AG055500 Rh5BG053100 Rh5CG062900 Rh5DG052100
rosa_wichuraiana Rw5G005460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 123
AasI GACNNNNNNGTC 1 cut(s) 238
Acc36I ACCTGC 1 cut(s) 123
AccI GTMKAC 1 cut(s) 338
AclWI GGATC 2 cut(s) 26, 158
AfaI GTAC 2 cut(s) 222, 346
AgsI TTSAA 3 cut(s) 74, 152, 277
AjnI CCWGG 2 cut(s) 197, 439
AluBI AGCT 1 cut(s) 418
AluI AGCT 1 cut(s) 418
Alw26I GTCTC 2 cut(s) 26, 246
AlwI GGATC 2 cut(s) 26, 158
AoxI GGCC 1 cut(s) 442
AspLEI GCGC 1 cut(s) 305
AsuHPI GGTGA 1 cut(s) 23
BccI CCATC 3 cut(s) 261, 385, 444
BciT130I CCWGG 2 cut(s) 199, 441
BcoDI GTCTC 2 cut(s) 26, 246
BfaI CTAG 1 cut(s) 419
BfmI CTRYAG 1 cut(s) 375
BfuAI ACCTGC 1 cut(s) 123
BmcAI AGTACT 1 cut(s) 346
Bme1390I CCNGG 2 cut(s) 199, 441
BmrFI CCNGG 2 cut(s) 199, 441
BpuEI CTTGAG 1 cut(s) 40
Bse1I ACTGG 4 cut(s) 20, 40, 90, 342
BseBI CCWGG 2 cut(s) 199, 441
BseGI GGATG 1 cut(s) 436
BseMII CTCAG 2 cut(s) 257, 387
BseNI ACTGG 4 cut(s) 20, 40, 90, 342
BshFI GGCC 1 cut(s) 444
BslFI GGGAC 2 cut(s) 96, 275
BsmAI GTCTC 2 cut(s) 26, 246
BsmFI GGGAC 2 cut(s) 96, 275
BsnI GGCC 1 cut(s) 444
Bsp143I GATC 3 cut(s) 18, 59, 163
BspANI GGCC 1 cut(s) 444
BspCNI CTCAG 2 cut(s) 256, 388
BspMAI CTGCAG 1 cut(s) 379
BspMI ACCTGC 1 cut(s) 123
BspPI GGATC 2 cut(s) 26, 158
BspQI GCTCTTC 1 cut(s) 284
BsrI ACTGG 4 cut(s) 20, 40, 90, 342
BssMI GATC 3 cut(s) 18, 59, 163
Bst2UI CCWGG 2 cut(s) 199, 441
Bst4CI ACNGT 1 cut(s) 240
Bst6I CTCTTC 4 cut(s) 147, 284, 349, 381
BstC8I GCNNGC 1 cut(s) 130
BstDEI CTNAG 5 cut(s) 45, 243, 332, 396, 446
BstF5I GGATG 1 cut(s) 436
BstHHI GCGC 1 cut(s) 305
BstKTI GATC 3 cut(s) 21, 62, 166
BstMAI GTCTC 2 cut(s) 26, 246
BstMBI GATC 3 cut(s) 18, 59, 163
BstMWI GCNNNNNNNGC 1 cut(s) 300
BstNI CCWGG 2 cut(s) 199, 441
BstSCI CCNGG 2 cut(s) 197, 439
BstSFI CTRYAG 1 cut(s) 375
BstX2I RGATCY 1 cut(s) 18
BstYI RGATCY 1 cut(s) 18
BsuRI GGCC 1 cut(s) 444
BtsCI GGATG 1 cut(s) 436
BveI ACCTGC 1 cut(s) 123
Cac8I GCNNGC 1 cut(s) 130
CfoI GCGC 1 cut(s) 305
Csp6I GTAC 2 cut(s) 221, 345
CviJI RGCY 5 cut(s) 54, 395, 418, 432, 444
CviKI_1 RGCY 5 cut(s) 54, 395, 418, 432, 444
CviQI GTAC 2 cut(s) 221, 345
DdeI CTNAG 5 cut(s) 45, 243, 332, 396, 446
DpnI GATC 3 cut(s) 20, 61, 165
DpnII GATC 3 cut(s) 18, 59, 163
DrdI GACNNNNNNGTC 1 cut(s) 238
DseDI GACNNNNNNGTC 1 cut(s) 238
Eam1104I CTCTTC 4 cut(s) 147, 284, 349, 381
EarI CTCTTC 4 cut(s) 147, 284, 349, 381
Eco147I AGGCCT 1 cut(s) 444
EcoRII CCWGG 2 cut(s) 197, 439
FaiI YATR 1 cut(s) 314
FalI AAGNNNNNCTT 2 cut(s) 49, 81
FaqI GGGAC 2 cut(s) 96, 275
FblI GTMKAC 1 cut(s) 338
FokI GGATG 1 cut(s) 423
FspBI CTAG 1 cut(s) 419
GlaI GCGC 1 cut(s) 304
HaeIII GGCC 1 cut(s) 444
HhaI GCGC 1 cut(s) 305
Hin6I GCGC 1 cut(s) 303
HinP1I GCGC 1 cut(s) 303
HinfI GANTC 2 cut(s) 247, 371
HphI GGTGA 1 cut(s) 23
Hpy166II GTNNAC 1 cut(s) 339
Hpy188I TCNGA 2 cut(s) 246, 370
Hpy188III TCNNGA 1 cut(s) 57
Hpy8I GTNNAC 1 cut(s) 339
HpyAV CCTTC 1 cut(s) 71
HpyCH4III ACNGT 1 cut(s) 240
HpyCH4V TGCA 3 cut(s) 128, 206, 377
HpyF10VI GCNNNNNNNGC 1 cut(s) 300
HpyF3I CTNAG 5 cut(s) 45, 243, 332, 396, 446
HspAI GCGC 1 cut(s) 303
Kzo9I GATC 3 cut(s) 18, 59, 163
LguI GCTCTTC 1 cut(s) 284
LpnPI CCDG 7 cut(s) 21, 71, 118, 184, 211, 355, 426
MaeI CTAG 1 cut(s) 419
MaeIII GTNAC 2 cut(s) 11, 170
MalI GATC 3 cut(s) 20, 61, 165
MboI GATC 3 cut(s) 18, 59, 163
MboII GAAGA 7 cut(s) 57, 86, 164, 289, 301, 366, 398
MfeI CAATTG 1 cut(s) 216
MflI RGATCY 1 cut(s) 18
MluCI AATT 1 cut(s) 216
MnlI CCTC 6 cut(s) 44, 116, 177, 218, 252, 293
MspR9I CCNGG 2 cut(s) 199, 441
MunI CAATTG 1 cut(s) 216
MvaI CCWGG 2 cut(s) 199, 441
MwoI GCNNNNNNNGC 1 cut(s) 300
NdeII GATC 3 cut(s) 18, 59, 163
NmuCI GTSAC 2 cut(s) 11, 170
PaqCI CACCTGC 1 cut(s) 123
PceI AGGCCT 1 cut(s) 444
PciSI GCTCTTC 1 cut(s) 284
PfeI GAWTC 2 cut(s) 247, 371
Psp6I CCWGG 2 cut(s) 197, 439
PspGI CCWGG 2 cut(s) 197, 439
PstI CTGCAG 1 cut(s) 379
PsuI RGATCY 1 cut(s) 18
RsaI GTAC 2 cut(s) 222, 346
RsaNI GTAC 2 cut(s) 221, 345
SapI GCTCTTC 1 cut(s) 284
Sau3AI GATC 3 cut(s) 18, 59, 163
ScaI AGTACT 1 cut(s) 346
ScrFI CCNGG 2 cut(s) 199, 441
SetI ASST 8 cut(s) 13, 106, 127, 137, 203, 229, 338, 420
SfcI CTRYAG 1 cut(s) 375
SmlI CTYRAG 1 cut(s) 55
SmoI CTYRAG 1 cut(s) 55
Sse9I AATT 1 cut(s) 216
SseBI AGGCCT 1 cut(s) 444
SspMI CTAG 1 cut(s) 419
StuI AGGCCT 1 cut(s) 444
StyD4I CCNGG 2 cut(s) 197, 439
TaaI ACNGT 1 cut(s) 240
TaqII GACCGA 1 cut(s) 248
TasI AATT 1 cut(s) 216
TatI WGTACW 1 cut(s) 344
TfiI GAWTC 2 cut(s) 247, 371
TseFI GTSAC 2 cut(s) 11, 170
Tsp45I GTSAC 2 cut(s) 11, 170
TspDTI ATGAA 3 cut(s) 17, 342, 399
TspGWI ACGGA 1 cut(s) 323
XmiI GTMKAC 1 cut(s) 338
XspI CTAG 1 cut(s) 419
ZrmI AGTACT 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.