Rroxscaffold_1G00068920

Kelch

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0015578
Physical Location & Seq
Forward (+)
89981028 .. 89982576
1549 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00068920.1

Sequence Viewer

Length: 1125 bp
ATGGGTTCTCTCCCATCTCCACCAAGACCCTCTTCTCCACCACCAGCACCAACTCATTCTCTGTCCAACTACAGAGTCTGTGCCACATTTTGCTTGAGAGAGCCCTCACCCAACTTGAACATATCCAATTGGATCGAAATCTACGATCCATCAACCAACTCTTGGACTCATGTCACCTCAATCCCTGGCCTCGTTGAGAACCACATCTTGAAAGGTTTCTCCATGGTCTCAATGGGTGACTCACTTTACATAATTGGTGGTCGGTTGTGCCACAAGGAACTCTCTCACACTTCGGATGAGTGCAGCTCGCTTGTGGATATGGAAGTTCAAGTCTCATCATTGGTGTTGCGTTATAATGTTGCGACTGATCAATGGTCCACTTGTGCACCACTTGGTGTAGCCAGGTGTGATTTCGCCTGCACGGTTTCCGATAACAAAATCTACGTGGCGGGTGGAAAGACCACGCTGGCTTGTGCGAGAGGAATTTCGTCGGCTGAGCTGTACGACCCTGAGCTAGACAAGTGGATTCATATGCCAAACATGAGCACATTGAGGTACAAGTGCGTTGGTGTCACATGGCAAAACAAAATCTACATTCTGGGTGGATTTGCAGAGCCAGTGAGGAAAAACTCAGACGTGCCTCCTATAATGGTGCGCAGCTCTGCTGAGGTTTATGACACGCAAACAAAGAAATGGGAATTGATAATTGGAATGTGGCAATTGGATGTACCACCAAATCAAATTGTTGATGTGGGTGGGAGGCTATTCAGTTCCGGGGATTGCTTCAAGCAATGGAAGGGTCATATTGAATCATATGATGGTGAACTCAACATATGGAATGAGGTTGATGGGTCTCATCTCCACAGTTTAAACTCACTGATTTCATCAGATAACGAAATGTGGGCACATAGCCAAAGGCGTTACCTAACGATGGCACCCATCGGAGATCACTTGTATTTTTTGGCGATTCATGGACTAATGGACAACGATTTGCCGAGAACAATGTCGATTGTCCATAGATTCGACACTTCTGCCACGAGTAACAATTGGACAAGCATGGAGCCGATGGAGGAGGACAGGGAGAAAGAGCTTTGTGGACATGCTTGTGTTGTTCATCTTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_1

Protein Analysis

374

Amino Acids

41.83

Weight (kDa)

5.55

Isoelectric Point (pI)

51.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_ATRN-LZTR1 PF24981 37 - 204 4.1e-10 Attractin/LZTR1 beta-propeller
Kelch_KLHDC2_KLHL20_DRC7 PF24681 72 - 235 3.3e-09 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_FKB95 PF25210 74 - 286 1e-11 FKB95, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 110 - 204 5.6e-07 Attractin/LZTR1 beta-propeller
Beta-prop_Calicin PF13964 113 - 233 3.2e-09 Calicin, beta-propeller domain
NANM PF24996 117 - 204 8e-06 N-acetylneuraminate epimerase
Kelch_1 PF01344 134 - 182 4.6e-11 Kelch motif
Beta-prop_FBX42 PF13415 135 - 236 5.6e-08 FBX42, beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015578)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g27971
malus_domestica MD05G1322500.v1.1 MD10G1300400.v1.1
prunus_persica Prupe.4G040300_v2.0.a1
pyrus_communis pycom05g29670 pycom10g25320
rosa_chinensis RchiOBHm_Chr5g0007471
rosa_laevigata RLG00000031459
rosa_multiflora Rmu_ssc0000182.1_g000004
rosa_roxburghii Rroxscaffold_1G00068920
rosa_rugosa Rorug04G0427100
rosa_samantha Rh5AG055500 Rh5BG053100 Rh5CG062900 Rh5DG052100
rosa_wichuraiana Rw5G005460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 354
Acc16I TGCGCA 1 cut(s) 656
AccB1I GGYRCC 1 cut(s) 934
AccB7I CCANNNNNTGG 1 cut(s) 162
AciI CCGC 1 cut(s) 449
AclWI GGATC 2 cut(s) 140, 140
AcsI RAATTY 1 cut(s) 483
AdeI CACNNNGTG 1 cut(s) 395
AfaI GTAC 3 cut(s) 503, 557, 729
AfiI CCNNNNNNNGG 2 cut(s) 162, 931
AgsI TTSAA 5 cut(s) 118, 211, 329, 787, 809
AjiI CACGTC 1 cut(s) 637
AjnI CCWGG 2 cut(s) 184, 401
AjuI GAANNNNNNNTTGG 4 cut(s) 16, 48, 690, 722
AluBI AGCT 5 cut(s) 306, 499, 514, 660, 1090
AluI AGCT 5 cut(s) 306, 499, 514, 660, 1090
Alw21I GWGCWC 2 cut(s) 388, 548
Alw26I GTCTC 3 cut(s) 232, 337, 858
Alw44I GTGCAC 1 cut(s) 384
AlwI GGATC 2 cut(s) 140, 140
AlwNI CAGNNNCTG 1 cut(s) 78
AoxI GGCC 1 cut(s) 187
ApaLI GTGCAC 1 cut(s) 384
ApeKI GCWGC 2 cut(s) 303, 657
ApoI RAATTY 1 cut(s) 483
Asp700I GAANNNNTTC 1 cut(s) 215
AspLEI GCGC 1 cut(s) 657
AspS9I GGNCC 1 cut(s) 375
AsuC2I CCSGG 1 cut(s) 775
AsuHPI GGTGA 4 cut(s) 99, 166, 248, 833
AvaII GGWCC 1 cut(s) 375
BaeGI GKGCMC 2 cut(s) 388, 907
BanI GGYRCC 1 cut(s) 934
BanII GRGCYC 1 cut(s) 105
BauI CACGAG 1 cut(s) 1036
Bbv12I GWGCWC 2 cut(s) 388, 548
BbvCI CCTCAGC 1 cut(s) 666
BbvI GCAGC 2 cut(s) 315, 669
BccI CCATC 7 cut(s) 22, 157, 812, 842, 925, 947, 1060
BcgI CGANNNNNNTGC 2 cut(s) 1013, 1047
BciT130I CCWGG 2 cut(s) 186, 403
BclI TGATCA 1 cut(s) 367
BcnI CCSGG 1 cut(s) 775
BcoDI GTCTC 3 cut(s) 232, 337, 858
BfaI CTAG 1 cut(s) 515
BfmI CTRYAG 1 cut(s) 70
BisI GCNGC 2 cut(s) 304, 658
BlpI GCTNAGC 1 cut(s) 495
BlsI GCNGC 2 cut(s) 305, 659
Bme1390I CCNGG 3 cut(s) 186, 403, 775
Bme18I GGWCC 1 cut(s) 375
BmgBI CACGTC 1 cut(s) 637
BmgT120I GGNCC 1 cut(s) 375
BmiI GGNNCC 2 cut(s) 936, 1062
BmrFI CCNGG 3 cut(s) 186, 403, 775
BoxI GACNNNNGTC 1 cut(s) 170
BplI GAGNNNNNCTC 2 cut(s) 290, 322
Bpu10I CCTNAGC 2 cut(s) 510, 666
Bpu1102I GCTNAGC 1 cut(s) 495
BpuEI CTTGAG 1 cut(s) 115
BpuMI CCSGG 1 cut(s) 775
BsaAI YACGTR 1 cut(s) 445
BsaBI GATNNNNATC 1 cut(s) 137
BsaI GGTCTC 2 cut(s) 232, 858
BsaJI CCNNGG 3 cut(s) 184, 222, 774
Bsc4I CCNNNNNNNGG 2 cut(s) 162, 931
Bse1I ACTGG 1 cut(s) 617
Bse3DI GCAATG 1 cut(s) 797
Bse8I GATNNNNATC 1 cut(s) 137
BseBI CCWGG 2 cut(s) 186, 403
BseDI CCNNGG 3 cut(s) 184, 222, 774
BseGI GGATG 2 cut(s) 301, 730
BseJI GATNNNNATC 1 cut(s) 137
BseLI CCNNNNNNNGG 2 cut(s) 162, 931
BseMI GCAATG 1 cut(s) 797
BseMII CTCAG 4 cut(s) 486, 501, 645, 657
BseNI ACTGG 1 cut(s) 617
BseRI GAGGAG 1 cut(s) 1085
BseSI GKGCMC 2 cut(s) 388, 907
BseXI GCAGC 2 cut(s) 315, 669
BsgI GTGCAG 2 cut(s) 322, 403
BshFI GGCC 1 cut(s) 189
BshNI GGYRCC 1 cut(s) 934
BsiHKAI GWGCWC 2 cut(s) 388, 548
BsiSI CCGG 1 cut(s) 774
BslI CCNNNNNNNGG 2 cut(s) 162, 931
BsmAI GTCTC 3 cut(s) 232, 337, 858
BsnI GGCC 1 cut(s) 189
Bso31I GGTCTC 2 cut(s) 232, 858
Bsp1286I GDGCHC 4 cut(s) 105, 388, 548, 907
Bsp143I GATC 4 cut(s) 132, 145, 367, 946
Bsp1720I GCTNAGC 1 cut(s) 495
Bsp19I CCATGG 1 cut(s) 222
BspACI CCGC 1 cut(s) 449
BspANI GGCC 1 cut(s) 189
BspCNI CTCAG 4 cut(s) 487, 502, 644, 658
BspLI GGNNCC 2 cut(s) 936, 1062
BspPI GGATC 2 cut(s) 140, 140
BspT107I GGYRCC 1 cut(s) 934
BspTNI GGTCTC 2 cut(s) 232, 858
BsrDI GCAATG 1 cut(s) 797
BsrI ACTGG 1 cut(s) 617
BssECI CCNNGG 3 cut(s) 184, 222, 774
BssMI GATC 4 cut(s) 132, 145, 367, 946
BssSI CACGAG 1 cut(s) 1036
BssT1I CCWWGG 1 cut(s) 222
Bst2BI CACGAG 1 cut(s) 1036
Bst2UI CCWGG 2 cut(s) 186, 403
Bst4CI ACNGT 2 cut(s) 424, 866
Bst6I CTCTTC 1 cut(s) 37
BstBAI YACGTR 1 cut(s) 445
BstC8I GCNNGC 3 cut(s) 308, 418, 468
BstDEI CTNAG 5 cut(s) 495, 510, 631, 666, 1122
BstDSI CCRYGG 1 cut(s) 222
BstF5I GGATG 2 cut(s) 301, 730
BstHHI GCGC 1 cut(s) 657
BstKTI GATC 4 cut(s) 135, 148, 370, 949
BstMAI GTCTC 3 cut(s) 232, 337, 858
BstMBI GATC 4 cut(s) 132, 145, 367, 946
BstNI CCWGG 2 cut(s) 186, 403
BstNSI RCATGY 1 cut(s) 1103
BstPAI GACNNNNGTC 1 cut(s) 170
BstSCI CCNGG 3 cut(s) 184, 401, 773
BstSFI CTRYAG 1 cut(s) 70
BstSLI GKGCMC 2 cut(s) 388, 907
BstV1I GCAGC 2 cut(s) 315, 669
BsuRI GGCC 1 cut(s) 189
BtgI CCRYGG 1 cut(s) 222
BtrI CACGTC 1 cut(s) 637
BtsCI GGATG 2 cut(s) 301, 730
BtsIMutI CAGTG 2 cut(s) 624, 875
Cac8I GCNNGC 3 cut(s) 308, 418, 468
CaiI CAGNNNCTG 1 cut(s) 78
CfoI GCGC 1 cut(s) 657
Cfr13I GGNCC 1 cut(s) 375
Csp6I GTAC 3 cut(s) 502, 556, 728
CviAII CATG 7 cut(s) 170, 223, 541, 576, 971, 1057, 1100
CviQI GTAC 3 cut(s) 502, 556, 728
DdeI CTNAG 5 cut(s) 495, 510, 631, 666, 1122
DpnI GATC 4 cut(s) 134, 147, 369, 948
DpnII GATC 4 cut(s) 132, 145, 367, 946
DraI TTTAAA 1 cut(s) 870
DraIII CACNNNGTG 1 cut(s) 395
Eam1104I CTCTTC 1 cut(s) 37
EarI CTCTTC 1 cut(s) 37
Eco130I CCWWGG 1 cut(s) 222
Eco24I GRGCYC 1 cut(s) 105
Eco31I GGTCTC 2 cut(s) 232, 858
Eco47I GGWCC 1 cut(s) 375
EcoRII CCWGG 2 cut(s) 184, 401
EcoT14I CCWWGG 1 cut(s) 222
EcoT38I GRGCYC 1 cut(s) 105
ErhI CCWWGG 1 cut(s) 222
FaeI CATG 7 cut(s) 173, 226, 544, 579, 974, 1060, 1103
FalI AAGNNNNNCTT 2 cut(s) 16, 48
FatI CATG 7 cut(s) 169, 222, 540, 575, 970, 1056, 1099
FauI CCCGC 1 cut(s) 442
FauNDI CATATG 3 cut(s) 531, 814, 833
FbaI TGATCA 1 cut(s) 367
Fnu4HI GCNGC 2 cut(s) 304, 658
FokI GGATG 2 cut(s) 308, 737
FriOI GRGCYC 1 cut(s) 105
Fsp4HI GCNGC 2 cut(s) 304, 658
FspBI CTAG 1 cut(s) 515
FspI TGCGCA 1 cut(s) 656
GlaI GCGC 1 cut(s) 656
GluI GCNGC 2 cut(s) 304, 658
HaeIII GGCC 1 cut(s) 189
HapII CCGG 1 cut(s) 774
HhaI GCGC 1 cut(s) 657
Hin1II CATG 7 cut(s) 173, 226, 544, 579, 974, 1060, 1103
Hin6I GCGC 1 cut(s) 655
HinP1I GCGC 1 cut(s) 655
HinfI GANTC 7 cut(s) 75, 166, 239, 526, 809, 967, 1020
HpaII CCGG 1 cut(s) 774
HphI GGTGA 4 cut(s) 99, 166, 248, 833
Hpy166II GTNNAC 4 cut(s) 378, 386, 824, 1097
Hpy188I TCNGA 5 cut(s) 295, 430, 634, 889, 944
Hpy188III TCNNGA 1 cut(s) 208
Hpy8I GTNNAC 4 cut(s) 378, 386, 824, 1097
Hpy99I CGWCG 1 cut(s) 493
HpyAV CCTTC 1 cut(s) 790
HpyCH4III ACNGT 2 cut(s) 424, 866
HpyCH4IV ACGT 2 cut(s) 444, 636
HpyCH4V TGCA 4 cut(s) 303, 386, 420, 611
HpyF3I CTNAG 5 cut(s) 495, 510, 631, 666, 1122
HpySE526I ACGT 2 cut(s) 444, 636
Hsp92II CATG 7 cut(s) 173, 226, 544, 579, 974, 1060, 1103
HspAI GCGC 1 cut(s) 655
Ksp22I TGATCA 1 cut(s) 367
Kzo9I GATC 4 cut(s) 132, 145, 367, 946
LmnI GCTCC 1 cut(s) 1060
Lsp1109I GCAGC 2 cut(s) 315, 669
MaeI CTAG 1 cut(s) 515
MaeII ACGT 2 cut(s) 444, 636
MaeIII GTNAC 5 cut(s) 172, 236, 571, 920, 1040
MalI GATC 4 cut(s) 134, 147, 369, 948
MboI GATC 4 cut(s) 132, 145, 367, 946
MboII GAAGA 1 cut(s) 24
MfeI CAATTG 3 cut(s) 127, 719, 1045
MhlI GDGCHC 4 cut(s) 105, 388, 548, 907
MluCI AATT 8 cut(s) 127, 252, 483, 698, 705, 719, 741, 1045
MlyI GAGTC 3 cut(s) 84, 160, 233
MmeI TCCRAC 1 cut(s) 90
MroXI GAANNNNTTC 1 cut(s) 215
MseI TTAA 1 cut(s) 869
MslI CAYNNNNRTG 1 cut(s) 1104
MspI CCGG 1 cut(s) 774
MspR9I CCNGG 3 cut(s) 186, 403, 775
MssI GTTTAAAC 1 cut(s) 870
MunI CAATTG 3 cut(s) 127, 719, 1045
MvaI CCWGG 2 cut(s) 186, 403
NciI CCSGG 1 cut(s) 775
NcoI CCATGG 1 cut(s) 222
NdeI CATATG 3 cut(s) 531, 814, 833
NdeII GATC 4 cut(s) 132, 145, 367, 946
NlaIII CATG 7 cut(s) 173, 226, 544, 579, 974, 1060, 1103
NlaIV GGNNCC 2 cut(s) 936, 1062
NmeAIII GCCGAG 1 cut(s) 1020
NmuCI GTSAC 3 cut(s) 172, 236, 571
NsbI TGCGCA 1 cut(s) 656
NspI RCATGY 1 cut(s) 1103
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PdmI GAANNNNTTC 1 cut(s) 215
PfeI GAWTC 4 cut(s) 526, 809, 967, 1020
PflMI CCANNNNNTGG 1 cut(s) 162
PkrI GCNGC 2 cut(s) 305, 659
PleI GAGTC 3 cut(s) 83, 160, 233
PmeI GTTTAAAC 1 cut(s) 870
PpsI GAGTC 3 cut(s) 83, 160, 233
Ppu21I YACGTR 1 cut(s) 445
PshAI GACNNNNGTC 1 cut(s) 170
PsiI TTATAA 1 cut(s) 354
Psp6I CCWGG 2 cut(s) 184, 401
PspGI CCWGG 2 cut(s) 184, 401
PspN4I GGNNCC 2 cut(s) 936, 1062
PspPI GGNCC 1 cut(s) 375
PstNI CAGNNNCTG 1 cut(s) 78
RsaI GTAC 3 cut(s) 503, 557, 729
RsaNI GTAC 3 cut(s) 502, 556, 728
RseI CAYNNNNRTG 1 cut(s) 1104
SaqAI TTAA 1 cut(s) 869
SatI GCNGC 2 cut(s) 304, 658
Sau3AI GATC 4 cut(s) 132, 145, 367, 946
Sau96I GGNCC 1 cut(s) 375
SchI GAGTC 3 cut(s) 84, 160, 233
ScrFI CCNGG 3 cut(s) 186, 403, 775
SduI GDGCHC 4 cut(s) 105, 388, 548, 907
SfcI CTRYAG 1 cut(s) 70
SinI GGWCC 1 cut(s) 375
SmiMI CAYNNNNRTG 1 cut(s) 1104
SmlI CTYRAG 1 cut(s) 94
SmoI CTYRAG 1 cut(s) 94
Sse9I AATT 8 cut(s) 127, 252, 483, 698, 705, 719, 741, 1045
SsiI CCGC 1 cut(s) 449
SspMI CTAG 1 cut(s) 515
StyD4I CCNGG 3 cut(s) 184, 401, 773
StyI CCWWGG 1 cut(s) 222
TaaI ACNGT 2 cut(s) 424, 866
TaiI ACGT 2 cut(s) 447, 639
TaqI TCGA 3 cut(s) 135, 1007, 1023
TasI AATT 8 cut(s) 127, 252, 483, 698, 705, 719, 741, 1045
TfiI GAWTC 4 cut(s) 526, 809, 967, 1020
Tru1I TTAA 1 cut(s) 869
Tru9I TTAA 1 cut(s) 869
TscAI CASTG 2 cut(s) 624, 882
TseFI GTSAC 3 cut(s) 172, 236, 571
TseI GCWGC 2 cut(s) 303, 657
Tsp45I GTSAC 3 cut(s) 172, 236, 571
TspDTI ATGAA 4 cut(s) 518, 873, 959, 1103
TspRI CASTG 2 cut(s) 624, 882
Van91I CCANNNNNTGG 1 cut(s) 162
VneI GTGCAC 1 cut(s) 384
VpaK11BI GGWCC 1 cut(s) 375
XapI RAATTY 1 cut(s) 483
XceI RCATGY 1 cut(s) 1103
XcmI CCANNNNNNNNNTGG 1 cut(s) 229
XmnI GAANNNNTTC 1 cut(s) 215
XspI CTAG 1 cut(s) 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.