pycom10g25320

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0015578
Physical Location & Seq
Forward (+)
26985073 .. 26986191
1119 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g25320.1

Sequence Viewer

Length: 1119 bp
ATGGGTTCTCTCCCATTTCCACCAAGACCCTCAACTCCACCACCCTCTTCGCCAAACCCTAATTCTCTATCCAAATTTAGGGTTTGTGCCACGTTTTGCTATAGAGAGCCCGCTCCAAACCTAAACATTTCTAATTGGATAGAATGCTACGATCCGTCGACCAACGTGTGGACGCATGCCAGCTCCATCCCAGGCCTCACTGAGAATCATGTCCTCAAGGGTTTTGCCATGGTGTCTTTACGTGATTCGGTTTACATAATTGGCGGTAGGGTTTGTAATAAGGAGAGAGCTCACACGTCGGACGAGTGCAGCGAGCTTGTCGACATGGACATTGAAGTCTCCTCCTCGGTCTTGCGCTACAATGTCGGGTCAAACCAGTGGTCTACCTGCGCGCCACTTGGCATCCCCCGGTGCGACTTCGCATGCACGGTCTCAGATAACAAGATCTACGTGGCGGGGGGAAAGGCCACGCTAGATTGGGCGAGGGGCATCCCATTCGCTGAGGTGTACGATCCGGACCACGACGAGTGGACACCGCTGCCGAACATGAGCACGTTGAGGTACAAGTGCGTTGGGGTGACGTGGATGGGCAAGATCTACGTGGTGGGGGGTTTCGCTGAGAAGGCTGACTCGGACGTGCCGGCCATCATGCTGCGCAGCTCCGCTGAGGTGTACGACCCGCTAGTAGGGAAGTGGGGCCTTATCGTGGGGATGTGGCAGCTGGACGTGCCACCTAATCAAATTTTGGCCGTCGATGGACAGCTTTTCAGCTCGGGCGATTGCTACAAGCAATGGAAAGGCCACATAGAATCCTACGACGGCAAGCTCAACATATGGAATGAAGTTGAAGGGTCCCGCCTACAAAGCCTCACTGCATTGGGGCCACACGATCAAAACTGGGCACTGAGCCAAAGGCGTTACCTAACAATGGCAACCATTGGGGTGGATTTATACTTCTTGGCAGGGTATGGGATGGAGAGCAAATCATCAAGAACAATGTCGATCGTTCATAAATTTGATATATCTGCAACCAACGATGCATGGACGAGCATGGAGCCGATGGAGGAAGATGGAGAGAAAGAGCTTTGTGGGCATTGCTGTGTTGTTCAACTCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_1

Protein Analysis

373

Amino Acids

41.22

Weight (kDa)

5.07

Isoelectric Point (pI)

45.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 46 - 204 1.1e-07 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_FKB95 PF25210 111 - 285 2.2e-14 FKB95, Kelch-repeats domain
Beta-prop_Calicin PF13964 112 - 236 2.3e-09 Calicin, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 112 - 213 1.1e-06 Attractin/LZTR1 beta-propeller
Kelch_1 PF01344 136 - 184 1.6e-09 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015578)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g27971
malus_domestica MD05G1322500.v1.1 MD10G1300400.v1.1
prunus_persica Prupe.4G040300_v2.0.a1
pyrus_communis pycom05g29670 pycom10g25320
rosa_chinensis RchiOBHm_Chr5g0007471
rosa_laevigata RLG00000031459
rosa_multiflora Rmu_ssc0000182.1_g000004
rosa_roxburghii Rroxscaffold_1G00068920
rosa_rugosa Rorug04G0427100
rosa_samantha Rh5AG055500 Rh5BG053100 Rh5CG062900 Rh5DG052100
rosa_wichuraiana Rw5G005460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 335
Acc16I TGCGCA 1 cut(s) 656
Acc36I ACCTGC 1 cut(s) 395
AccB7I CCANNNNNTGG 1 cut(s) 168
AccBSI CCGCTC 1 cut(s) 113
AccI GTMKAC 3 cut(s) 158, 321, 383
AccII CGCG 1 cut(s) 392
AccIII TCCGGA 1 cut(s) 514
AciI CCGC 7 cut(s) 111, 264, 455, 536, 663, 680, 856
AclWI GGATC 2 cut(s) 146, 506
AcoI YGGCCR 2 cut(s) 642, 747
AcsI RAATTY 3 cut(s) 74, 741, 1013
AfaI GTAC 3 cut(s) 509, 563, 674
AfiI CCNNNNNNNGG 5 cut(s) 78, 168, 686, 706, 928
AflIII ACRYGT 2 cut(s) 165, 294
AgsI TTSAA 3 cut(s) 335, 848, 1109
AjiI CACGTC 4 cut(s) 297, 582, 637, 727
AjnI CCWGG 1 cut(s) 190
AjuI GAANNNNNNNTTGG 2 cut(s) 46, 78
AluBI AGCT 9 cut(s) 183, 290, 316, 660, 721, 763, 771, 826, 1084
AluI AGCT 9 cut(s) 183, 290, 316, 660, 721, 763, 771, 826, 1084
Alw21I GWGCWC 2 cut(s) 292, 554
Alw26I GTCTC 2 cut(s) 343, 436
AlwI GGATC 2 cut(s) 146, 506
Ama87I CYCGRG 1 cut(s) 772
Aor13HI TCCGGA 1 cut(s) 514
AoxI GGCC 7 cut(s) 193, 465, 642, 697, 747, 799, 881
ApeKI GCWGC 5 cut(s) 309, 538, 652, 657, 718
ApoI RAATTY 3 cut(s) 74, 741, 1013
ArsI GACNNNNNNTTYG 2 cut(s) 365, 397
AspLEI GCGC 4 cut(s) 357, 392, 394, 657
AspS9I GGNCC 4 cut(s) 517, 697, 852, 881
AsuC2I CCSGG 1 cut(s) 409
AsuHPI GGTGA 1 cut(s) 589
AvaI CYCGRG 1 cut(s) 772
AvaII GGWCC 2 cut(s) 517, 852
BaeGI GKGCMC 1 cut(s) 904
BanII GRGCYC 2 cut(s) 111, 292
Bbv12I GWGCWC 2 cut(s) 292, 554
BbvCI CCTCAGC 2 cut(s) 501, 666
BbvI GCAGC 5 cut(s) 321, 525, 639, 669, 730
BccI CCATC 7 cut(s) 194, 580, 653, 749, 967, 1054, 1064
BceAI ACGGC 2 cut(s) 734, 835
BcgI CGANNNNNNTGC 2 cut(s) 478, 512
BciT130I CCWGG 1 cut(s) 192
BcnI CCSGG 1 cut(s) 409
BcoDI GTCTC 2 cut(s) 343, 436
BfaI CTAG 2 cut(s) 473, 683
BfmI CTRYAG 1 cut(s) 100
BfuAI ACCTGC 1 cut(s) 395
BglII AGATCT 2 cut(s) 444, 594
BisI GCNGC 5 cut(s) 310, 539, 653, 658, 719
BlsI GCNGC 5 cut(s) 311, 540, 654, 659, 720
Bme1390I CCNGG 2 cut(s) 192, 409
Bme18I GGWCC 2 cut(s) 517, 852
BmeT110I CYCGRG 1 cut(s) 772
BmgBI CACGTC 4 cut(s) 297, 582, 637, 727
BmgT120I GGNCC 4 cut(s) 517, 697, 852, 881
BmiI GGNNCC 5 cut(s) 698, 853, 854, 882, 1056
BmrFI CCNGG 2 cut(s) 192, 409
BmrI ACTGGG 1 cut(s) 907
BmsI GCATC 3 cut(s) 411, 498, 1027
BmuI ACTGGG 1 cut(s) 907
Bpu10I CCTNAGC 2 cut(s) 501, 666
BpuEI CTTGAG 1 cut(s) 200
BpuMI CCSGG 1 cut(s) 409
BsaAI YACGTR 3 cut(s) 242, 451, 601
BsaI GGTCTC 1 cut(s) 436
BsaJI CCNNGG 4 cut(s) 190, 228, 345, 407
BsaWI WCCGGW 1 cut(s) 514
Bsc4I CCNNNNNNNGG 5 cut(s) 78, 168, 686, 706, 928
Bse118I RCCGGY 1 cut(s) 640
Bse1I ACTGG 2 cut(s) 376, 902
Bse3DI GCAATG 2 cut(s) 797, 1093
BseAI TCCGGA 1 cut(s) 514
BseBI CCWGG 1 cut(s) 192
BseDI CCNNGG 4 cut(s) 190, 228, 345, 407
BseGI GGATG 6 cut(s) 186, 402, 489, 591, 717, 978
BseLI CCNNNNNNNGG 5 cut(s) 78, 168, 686, 706, 928
BseMI GCAATG 2 cut(s) 797, 1093
BseMII CTCAG 6 cut(s) 192, 447, 492, 609, 657, 896
BseNI ACTGG 2 cut(s) 376, 902
BsePI GCGCGC 1 cut(s) 390
BseRI GAGGAG 2 cut(s) 331, 334
BseSI GKGCMC 1 cut(s) 904
BseXI GCAGC 5 cut(s) 321, 525, 639, 669, 730
BsgI GTGCAG 1 cut(s) 328
Bsh1236I CGCG 1 cut(s) 392
Bsh1285I CGRYCG 1 cut(s) 1005
BshFI GGCC 7 cut(s) 195, 467, 644, 699, 749, 801, 883
BsiEI CGRYCG 1 cut(s) 1005
BsiHKAI GWGCWC 2 cut(s) 292, 554
BsiHKCI CYCGRG 1 cut(s) 772
BsiSI CCGG 3 cut(s) 409, 515, 641
BslFI GGGAC 1 cut(s) 838
BslI CCNNNNNNNGG 5 cut(s) 78, 168, 686, 706, 928
BsmAI GTCTC 2 cut(s) 343, 436
BsmFI GGGAC 1 cut(s) 838
BsmI GAATGC 1 cut(s) 149
BsnI GGCC 7 cut(s) 195, 467, 644, 699, 749, 801, 883
Bso31I GGTCTC 1 cut(s) 436
BsoBI CYCGRG 1 cut(s) 772
Bsp1286I GDGCHC 4 cut(s) 111, 292, 554, 904
Bsp13I TCCGGA 1 cut(s) 514
Bsp143I GATC 6 cut(s) 151, 444, 511, 594, 889, 1002
Bsp19I CCATGG 1 cut(s) 228
BspACI CCGC 7 cut(s) 111, 264, 455, 536, 663, 680, 856
BspANI GGCC 7 cut(s) 195, 467, 644, 699, 749, 801, 883
BspCNI CTCAG 6 cut(s) 193, 446, 493, 610, 658, 897
BspEI TCCGGA 1 cut(s) 514
BspFNI CGCG 1 cut(s) 392
BspHI TCATGA 1 cut(s) 1115
BspLI GGNNCC 5 cut(s) 698, 853, 854, 882, 1056
BspMI ACCTGC 1 cut(s) 395
BspPI GGATC 2 cut(s) 146, 506
BspTNI GGTCTC 1 cut(s) 436
BsrBI CCGCTC 1 cut(s) 113
BsrDI GCAATG 2 cut(s) 797, 1093
BsrFI RCCGGY 1 cut(s) 640
BsrI ACTGG 2 cut(s) 376, 902
BssAI RCCGGY 1 cut(s) 640
BssECI CCNNGG 4 cut(s) 190, 228, 345, 407
BssHII GCGCGC 1 cut(s) 390
BssMI GATC 6 cut(s) 151, 444, 511, 594, 889, 1002
BssT1I CCWWGG 1 cut(s) 228
Bst2UI CCWGG 1 cut(s) 192
Bst4CI ACNGT 1 cut(s) 430
Bst6I CTCTTC 1 cut(s) 52
BstBAI YACGTR 3 cut(s) 242, 451, 601
BstC8I GCNNGC 8 cut(s) 111, 177, 181, 314, 392, 424, 642, 824
BstDEI CTNAG 6 cut(s) 201, 433, 501, 618, 666, 905
BstDSI CCRYGG 1 cut(s) 228
BstF5I GGATG 6 cut(s) 186, 402, 489, 591, 717, 978
BstFNI CGCG 1 cut(s) 392
BstHHI GCGC 4 cut(s) 357, 392, 394, 657
BstKTI GATC 6 cut(s) 154, 447, 514, 597, 892, 1005
BstMAI GTCTC 2 cut(s) 343, 436
BstMBI GATC 6 cut(s) 151, 444, 511, 594, 889, 1002
BstMCI CGRYCG 1 cut(s) 1005
BstMWI GCNNNNNNNGC 4 cut(s) 623, 727, 864, 1090
BstNI CCWGG 1 cut(s) 192
BstNSI RCATGY 2 cut(s) 179, 426
BstSCI CCNGG 2 cut(s) 190, 407
BstSFI CTRYAG 1 cut(s) 100
BstSLI GKGCMC 1 cut(s) 904
BstUI CGCG 1 cut(s) 392
BstV1I GCAGC 5 cut(s) 321, 525, 639, 669, 730
BstX2I RGATCY 2 cut(s) 444, 594
BstXI CCANNNNNNTGG 1 cut(s) 943
BstYI RGATCY 2 cut(s) 444, 594
BsuRI GGCC 7 cut(s) 195, 467, 644, 699, 749, 801, 883
BtgI CCRYGG 1 cut(s) 228
BtrI CACGTC 4 cut(s) 297, 582, 637, 727
BtsCI GGATG 6 cut(s) 186, 402, 489, 591, 717, 978
BtsI GCAGTG 1 cut(s) 870
BtsIMutI CAGTG 4 cut(s) 198, 383, 870, 902
BveI ACCTGC 1 cut(s) 395
Cac8I GCNNGC 8 cut(s) 111, 177, 181, 314, 392, 424, 642, 824
CciI TCATGA 1 cut(s) 1115
CfoI GCGC 4 cut(s) 357, 392, 394, 657
Cfr10I RCCGGY 1 cut(s) 640
Cfr13I GGNCC 4 cut(s) 517, 697, 852, 881
CseI GACGC 1 cut(s) 181
Csp6I GTAC 3 cut(s) 508, 562, 673
CviQI GTAC 3 cut(s) 508, 562, 673
DdeI CTNAG 6 cut(s) 201, 433, 501, 618, 666, 905
DpnI GATC 6 cut(s) 153, 446, 513, 596, 891, 1004
DpnII GATC 6 cut(s) 151, 444, 511, 594, 889, 1002
DrdI GACNNNNNNGTC 1 cut(s) 335
DseDI GACNNNNNNGTC 1 cut(s) 335
EaeI YGGCCR 2 cut(s) 642, 747
Eam1104I CTCTTC 1 cut(s) 52
EarI CTCTTC 1 cut(s) 52
Ecl136II GAGCTC 1 cut(s) 290
Eco130I CCWWGG 1 cut(s) 228
Eco147I AGGCCT 1 cut(s) 195
Eco24I GRGCYC 2 cut(s) 111, 292
Eco31I GGTCTC 1 cut(s) 436
Eco47I GGWCC 2 cut(s) 517, 852
Eco53kI GAGCTC 1 cut(s) 290
Eco88I CYCGRG 1 cut(s) 772
EcoICRI GAGCTC 1 cut(s) 290
EcoO109I RGGNCCY 2 cut(s) 697, 852
EcoRII CCWGG 1 cut(s) 190
EcoT14I CCWWGG 1 cut(s) 228
EcoT22I ATGCAT 1 cut(s) 1042
EcoT38I GRGCYC 2 cut(s) 111, 292
ErhI CCWWGG 1 cut(s) 228
FaqI GGGAC 1 cut(s) 838
FauI CCCGC 4 cut(s) 118, 448, 687, 863
FauNDI CATATG 1 cut(s) 833
FblI GTMKAC 3 cut(s) 158, 321, 383
Fnu4HI GCNGC 5 cut(s) 310, 539, 653, 658, 719
FokI GGATG 6 cut(s) 173, 389, 476, 598, 724, 985
FriOI GRGCYC 2 cut(s) 111, 292
Fsp4HI GCNGC 5 cut(s) 310, 539, 653, 658, 719
FspBI CTAG 2 cut(s) 473, 683
FspI TGCGCA 1 cut(s) 656
GlaI GCGC 4 cut(s) 356, 391, 393, 656
GluI GCNGC 5 cut(s) 310, 539, 653, 658, 719
HaeIII GGCC 7 cut(s) 195, 467, 644, 699, 749, 801, 883
HapII CCGG 3 cut(s) 409, 515, 641
HgaI GACGC 1 cut(s) 181
HhaI GCGC 4 cut(s) 357, 392, 394, 657
Hin6I GCGC 4 cut(s) 355, 390, 392, 655
HinP1I GCGC 4 cut(s) 355, 390, 392, 655
HincII GTYRAC 2 cut(s) 159, 322
HindII GTYRAC 2 cut(s) 159, 322
HinfI GANTC 4 cut(s) 205, 245, 629, 809
HpaII CCGG 3 cut(s) 409, 515, 641
HphI GGTGA 1 cut(s) 589
Hpy166II GTNNAC 8 cut(s) 159, 171, 253, 322, 384, 508, 531, 673
Hpy188I TCNGA 3 cut(s) 301, 436, 634
Hpy188III TCNNGA 3 cut(s) 515, 990, 1116
Hpy8I GTNNAC 8 cut(s) 159, 171, 253, 322, 384, 508, 531, 673
Hpy99I CGWCG 5 cut(s) 160, 301, 527, 755, 821
HpyAV CCTTC 2 cut(s) 616, 842
HpyCH4III ACNGT 1 cut(s) 430
HpyCH4V TGCA 5 cut(s) 309, 426, 875, 1028, 1040
HpyF10VI GCNNNNNNNGC 4 cut(s) 623, 727, 864, 1090
HpyF3I CTNAG 6 cut(s) 201, 433, 501, 618, 666, 905
HspAI GCGC 4 cut(s) 355, 390, 392, 655
KflI GGGWCCC 1 cut(s) 852
Kpn2I TCCGGA 1 cut(s) 514
KroI GCCGGC 1 cut(s) 640
KroNI GCCGGC 1 cut(s) 642
Kzo9I GATC 6 cut(s) 151, 444, 511, 594, 889, 1002
LmnI GCTCC 4 cut(s) 118, 188, 665, 1054
Lsp1109I GCAGC 5 cut(s) 321, 525, 639, 669, 730
LweI GCATC 3 cut(s) 411, 498, 1027
MaeI CTAG 2 cut(s) 473, 683
MaeIII GTNAC 2 cut(s) 577, 917
MalI GATC 6 cut(s) 153, 446, 513, 596, 891, 1004
MbiI CCGCTC 1 cut(s) 113
MboI GATC 6 cut(s) 151, 444, 511, 594, 889, 1002
MboII GAAGA 2 cut(s) 39, 1079
MflI RGATCY 2 cut(s) 444, 594
MhlI GDGCHC 4 cut(s) 111, 292, 554, 904
MluCI AATT 6 cut(s) 61, 74, 133, 258, 741, 1013
MlyI GAGTC 1 cut(s) 623
MmeI TCCRAC 1 cut(s) 279
Mph1103I ATGCAT 1 cut(s) 1042
MroI TCCGGA 1 cut(s) 514
MroNI GCCGGC 1 cut(s) 640
MslI CAYNNNNRTG 2 cut(s) 941, 1098
MspA1I CMGCKG 3 cut(s) 538, 665, 721
MspI CCGG 3 cut(s) 409, 515, 641
MspR9I CCNGG 2 cut(s) 192, 409
Mva1269I GAATGC 1 cut(s) 149
MvaI CCWGG 1 cut(s) 192
MvnI CGCG 1 cut(s) 392
MwoI GCNNNNNNNGC 4 cut(s) 623, 727, 864, 1090
NaeI GCCGGC 1 cut(s) 642
NciI CCSGG 1 cut(s) 409
NcoI CCATGG 1 cut(s) 228
NdeI CATATG 1 cut(s) 833
NdeII GATC 6 cut(s) 151, 444, 511, 594, 889, 1002
NgoMIV GCCGGC 1 cut(s) 640
NlaIV GGNNCC 5 cut(s) 698, 853, 854, 882, 1056
NmuCI GTSAC 1 cut(s) 577
NsbI TGCGCA 1 cut(s) 656
NsiI ATGCAT 1 cut(s) 1042
NspI RCATGY 2 cut(s) 179, 426
PaeI GCATGC 2 cut(s) 179, 426
PagI TCATGA 1 cut(s) 1115
PauI GCGCGC 1 cut(s) 390
PceI AGGCCT 1 cut(s) 195
PcsI WCGNNNNNNNCGW 1 cut(s) 309
PctI GAATGC 1 cut(s) 149
PdiI GCCGGC 1 cut(s) 642
PfeI GAWTC 3 cut(s) 205, 245, 809
PflMI CCANNNNNTGG 1 cut(s) 168
PkrI GCNGC 5 cut(s) 311, 540, 654, 659, 720
Ple19I CGATCG 1 cut(s) 1005
PleI GAGTC 1 cut(s) 623
PpsI GAGTC 1 cut(s) 623
Ppu21I YACGTR 3 cut(s) 242, 451, 601
PpuMI RGGWCCY 1 cut(s) 852
Psp124BI GAGCTC 1 cut(s) 292
Psp5II RGGWCCY 1 cut(s) 852
Psp6I CCWGG 1 cut(s) 190
PspGI CCWGG 1 cut(s) 190
PspN4I GGNNCC 5 cut(s) 698, 853, 854, 882, 1056
PspPI GGNCC 4 cut(s) 517, 697, 852, 881
PspPPI RGGWCCY 1 cut(s) 852
PsuI RGATCY 2 cut(s) 444, 594
PteI GCGCGC 1 cut(s) 390
PvuI CGATCG 1 cut(s) 1005
PvuII CAGCTG 1 cut(s) 721
RsaI GTAC 3 cut(s) 509, 563, 674
RsaNI GTAC 3 cut(s) 508, 562, 673
RseI CAYNNNNRTG 2 cut(s) 941, 1098
SacI GAGCTC 1 cut(s) 292
SalI GTCGAC 2 cut(s) 157, 320
SatI GCNGC 5 cut(s) 310, 539, 653, 658, 719
Sau3AI GATC 6 cut(s) 151, 444, 511, 594, 889, 1002
Sau96I GGNCC 4 cut(s) 517, 697, 852, 881
SchI GAGTC 1 cut(s) 623
ScrFI CCNGG 2 cut(s) 192, 409
SduI GDGCHC 4 cut(s) 111, 292, 554, 904
SfaNI GCATC 3 cut(s) 411, 498, 1027
SfcI CTRYAG 1 cut(s) 100
SinI GGWCC 2 cut(s) 517, 852
SmiMI CAYNNNNRTG 2 cut(s) 941, 1098
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
SphI GCATGC 2 cut(s) 179, 426
Sse9I AATT 6 cut(s) 61, 74, 133, 258, 741, 1013
SseBI AGGCCT 1 cut(s) 195
SsiI CCGC 7 cut(s) 111, 264, 455, 536, 663, 680, 856
SspMI CTAG 2 cut(s) 473, 683
SstI GAGCTC 1 cut(s) 292
StuI AGGCCT 1 cut(s) 195
StyD4I CCNGG 2 cut(s) 190, 407
StyI CCWWGG 1 cut(s) 228
TaaI ACNGT 1 cut(s) 430
TaqI TCGA 4 cut(s) 158, 321, 753, 1001
TaqII GACCGA 1 cut(s) 337
TasI AATT 6 cut(s) 61, 74, 133, 258, 741, 1013
TfiI GAWTC 3 cut(s) 205, 245, 809
TscAI CASTG 4 cut(s) 205, 383, 877, 909
TseFI GTSAC 1 cut(s) 577
TseI GCWGC 5 cut(s) 309, 538, 652, 657, 718
Tsp45I GTSAC 1 cut(s) 577
TspDTI ATGAA 2 cut(s) 855, 998
TspGWI ACGGA 1 cut(s) 144
TspRI CASTG 4 cut(s) 205, 383, 877, 909
Van91I CCANNNNNTGG 1 cut(s) 168
VpaK11BI GGWCC 2 cut(s) 517, 852
XapI RAATTY 3 cut(s) 74, 741, 1013
XceI RCATGY 2 cut(s) 179, 426
XmiI GTMKAC 3 cut(s) 158, 321, 383
XspI CTAG 2 cut(s) 473, 683
Zsp2I ATGCAT 1 cut(s) 1042
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.