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| Species | Orthologous Gene IDs |
|---|---|
| fragaria_vesca | FvH4_3g28450 FvH4_3g28450 |
| malus_domestica | MD03G1172000.v1.1 |
| prunus_persica | Prupe.4G254900_v2.0.a1 Prupe.4G254900_v2.0.a1 |
| pyrus_communis | pycom03g12910 |
| rosa_chinensis | RchiOBHm_Chr5g0053011 |
| rosa_laevigata | RLG00000034778 |
| rosa_multiflora | Rmu_sc0006573.1_g000003 |
| rosa_roxburghii | Rroxscaffold_1G00028830 |
| rosa_rugosa | Rorug05G0268800 |
| rosa_samantha | Rh5AG341100 Rh5BG351600 Rh5CG378000 Rh5DG368000 |
| rosa_wichuraiana | Rw5G032150 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 1199, 1346 |
| AarI | CACCTGC | 1 cut(s) | 320 |
| AbsI | CCTCGAGG | 1 cut(s) | 1393 |
| Acc36I | ACCTGC | 1 cut(s) | 320 |
| AccB1I | GGYRCC | 1 cut(s) | 2137 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 428 |
| AccI | GTMKAC | 1 cut(s) | 1639 |
| AclWI | GGATC | 4 cut(s) | 567, 652, 1385, 1956 |
| AcsI | RAATTY | 5 cut(s) | 771, 786, 1153, 1996, 2208 |
| AcuI | CTGAAG | 3 cut(s) | 954, 1493, 2028 |
| AfaI | GTAC | 7 cut(s) | 436, 768, 953, 1073, 1096, 1652, 1943 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 92, 428, 839, 1429, 1990, 1991 |
| AhlI | ACTAGT | 1 cut(s) | 1789 |
| AjnI | CCWGG | 6 cut(s) | 256, 832, 1081, 1717, 1876, 2081 |
| Alw21I | GWGCWC | 3 cut(s) | 59, 359, 1269 |
| Alw26I | GTCTC | 4 cut(s) | 160, 502, 923, 1695 |
| AlwI | GGATC | 4 cut(s) | 567, 652, 1385, 1956 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 932, 1597, 2121 |
| Ama87I | CYCGRG | 2 cut(s) | 1393, 2129 |
| AoxI | GGCC | 4 cut(s) | 1085, 1099, 1420, 1733 |
| ApeKI | GCWGC | 2 cut(s) | 272, 287 |
| ApoI | RAATTY | 5 cut(s) | 771, 786, 1153, 1996, 2208 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1039, 1071 |
| AspS9I | GGNCC | 6 cut(s) | 857, 971, 1085, 1421, 1880, 2241 |
| AsuC2I | CCSGG | 1 cut(s) | 1443 |
| AsuHPI | GGTGA | 3 cut(s) | 344, 727, 857 |
| AsuNHI | GCTAGC | 1 cut(s) | 1369 |
| AvaI | CYCGRG | 2 cut(s) | 1393, 2129 |
| AvaII | GGWCC | 4 cut(s) | 857, 971, 1880, 2241 |
| AxyI | CCTNAGG | 1 cut(s) | 1526 |
| BanI | GGYRCC | 1 cut(s) | 2137 |
| BanII | GRGCYC | 2 cut(s) | 59, 359 |
| BbsI | GAAGAC | 2 cut(s) | 817, 1048 |
| Bbv12I | GWGCWC | 3 cut(s) | 59, 359, 1269 |
| BbvCI | CCTCAGC | 1 cut(s) | 1256 |
| BbvI | GCAGC | 2 cut(s) | 274, 284 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 2051, 2085 |
| BciT130I | CCWGG | 6 cut(s) | 258, 834, 1083, 1719, 1878, 2083 |
| BclI | TGATCA | 1 cut(s) | 1588 |
| BcnI | CCSGG | 1 cut(s) | 1443 |
| BcoDI | GTCTC | 4 cut(s) | 160, 502, 923, 1695 |
| BcuI | ACTAGT | 1 cut(s) | 1789 |
| BfaI | CTAG | 9 cut(s) | 291, 800, 917, 1103, 1370, 1466, 1790, 1889, 2076 |
| BfmI | CTRYAG | 2 cut(s) | 327, 750 |
| BfuAI | ACCTGC | 1 cut(s) | 320 |
| BglII | AGATCT | 2 cut(s) | 1448, 2065 |
| BisI | GCNGC | 2 cut(s) | 273, 288 |
| BlsI | GCNGC | 2 cut(s) | 274, 289 |
| BmcAI | AGTACT | 2 cut(s) | 953, 1073 |
| Bme1390I | CCNGG | 7 cut(s) | 258, 834, 1083, 1443, 1719, 1878, 2083 |
| Bme18I | GGWCC | 4 cut(s) | 857, 971, 1880, 2241 |
| BmeT110I | CYCGRG | 2 cut(s) | 1393, 2129 |
| BmgT120I | GGNCC | 6 cut(s) | 857, 971, 1085, 1421, 1880, 2241 |
| BmiI | GGNNCC | 3 cut(s) | 973, 2139, 2243 |
| BmrFI | CCNGG | 7 cut(s) | 258, 834, 1083, 1443, 1719, 1878, 2083 |
| BmrI | ACTGGG | 2 cut(s) | 438, 1837 |
| BmtI | GCTAGC | 1 cut(s) | 1373 |
| BmuI | ACTGGG | 2 cut(s) | 438, 1837 |
| BpiI | GAAGAC | 2 cut(s) | 817, 1048 |
| BpmI | CTGGAG | 2 cut(s) | 373, 1860 |
| Bpu10I | CCTNAGC | 3 cut(s) | 402, 1256, 2163 |
| BpuEI | CTTGAG | 1 cut(s) | 672 |
| BpuMI | CCSGG | 1 cut(s) | 1443 |
| BsaJI | CCNNGG | 6 cut(s) | 256, 833, 1081, 1082, 1427, 2130 |
| BsaWI | WCCGGW | 1 cut(s) | 641 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1348, 1378, 1621, 1651 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 92, 428, 839, 1429, 1990, 1991 |
| Bse21I | CCTNAGG | 1 cut(s) | 1526 |
| Bse3DI | GCAATG | 4 cut(s) | 1443, 1734, 1739, 1818 |
| BseBI | CCWGG | 6 cut(s) | 258, 834, 1083, 1719, 1878, 2083 |
| BseDI | CCNNGG | 6 cut(s) | 256, 833, 1081, 1082, 1427, 2130 |
| BseGI | GGATG | 8 cut(s) | 132, 412, 727, 1438, 1477, 1822, 1826, 1883 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 92, 428, 839, 1429, 1990, 1991 |
| BseMI | GCAATG | 4 cut(s) | 1443, 1734, 1739, 1818 |
| BseMII | CTCAG | 7 cut(s) | 61, 416, 867, 1247, 1517, 1928, 2177 |
| BseRI | GAGGAG | 1 cut(s) | 1051 |
| BseXI | GCAGC | 2 cut(s) | 274, 284 |
| BsgI | GTGCAG | 5 cut(s) | 169, 940, 1308, 1369, 2038 |
| BshFI | GGCC | 4 cut(s) | 1087, 1101, 1422, 1735 |
| BshNI | GGYRCC | 1 cut(s) | 2137 |
| BsiHKAI | GWGCWC | 3 cut(s) | 59, 359, 1269 |
| BsiHKCI | CYCGRG | 2 cut(s) | 1393, 2129 |
| BsiSI | CCGG | 2 cut(s) | 642, 1442 |
| BslFI | GGGAC | 3 cut(s) | 211, 1800, 2227 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 92, 428, 839, 1429, 1990, 1991 |
| BsmAI | GTCTC | 4 cut(s) | 160, 502, 923, 1695 |
| BsmBI | CGTCTC | 1 cut(s) | 502 |
| BsmFI | GGGAC | 3 cut(s) | 211, 1800, 2227 |
| BsmI | GAATGC | 2 cut(s) | 949, 1813 |
| BsnI | GGCC | 4 cut(s) | 1087, 1101, 1422, 1735 |
| BsoBI | CYCGRG | 2 cut(s) | 1393, 2129 |
| Bsp1286I | GDGCHC | 3 cut(s) | 59, 359, 1269 |
| Bsp1407I | TGTACA | 1 cut(s) | 766 |
| Bsp19I | CCATGG | 1 cut(s) | 1427 |
| BspANI | GGCC | 4 cut(s) | 1087, 1101, 1422, 1735 |
| BspCNI | CTCAG | 7 cut(s) | 60, 415, 866, 1248, 1518, 1929, 2176 |
| BspHI | TCATGA | 1 cut(s) | 2303 |
| BspLI | GGNNCC | 3 cut(s) | 973, 2139, 2243 |
| BspMAI | CTGCAG | 1 cut(s) | 331 |
| BspMI | ACCTGC | 1 cut(s) | 320 |
| BspOI | GCTAGC | 1 cut(s) | 1373 |
| BspPI | GGATC | 4 cut(s) | 567, 652, 1385, 1956 |
| BspT107I | GGYRCC | 1 cut(s) | 2137 |
| BsrDI | GCAATG | 4 cut(s) | 1443, 1734, 1739, 1818 |
| BsrGI | TGTACA | 1 cut(s) | 766 |
| BssECI | CCNNGG | 6 cut(s) | 256, 833, 1081, 1082, 1427, 2130 |
| BssT1I | CCWWGG | 1 cut(s) | 1427 |
| Bst2UI | CCWGG | 6 cut(s) | 258, 834, 1083, 1719, 1878, 2083 |
| Bst4CI | ACNGT | 7 cut(s) | 474, 895, 1033, 1599, 1644, 1905, 1976 |
| Bst6I | CTCTTC | 1 cut(s) | 1484 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 269, 746, 2121 |
| BstAUI | TGTACA | 1 cut(s) | 766 |
| BstC8I | GCNNGC | 1 cut(s) | 1371 |
| BstDEI | CTNAG | 8 cut(s) | 47, 212, 402, 853, 1256, 1526, 1937, 2163 |
| BstDSI | CCRYGG | 1 cut(s) | 1427 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 837 |
| BstF5I | GGATG | 8 cut(s) | 132, 412, 727, 1438, 1477, 1822, 1826, 1883 |
| BstMAI | GTCTC | 4 cut(s) | 160, 502, 923, 1695 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 269, 278, 287, 407, 746, 1497, 1741, 2121 |
| BstNI | CCWGG | 6 cut(s) | 258, 834, 1083, 1719, 1878, 2083 |
| BstNSI | RCATGY | 1 cut(s) | 740 |
| BstSCI | CCNGG | 7 cut(s) | 256, 832, 1081, 1441, 1717, 1876, 2081 |
| BstSFI | CTRYAG | 2 cut(s) | 327, 750 |
| BstV1I | GCAGC | 2 cut(s) | 274, 284 |
| BstV2I | GAAGAC | 2 cut(s) | 817, 1048 |
| BstX2I | RGATCY | 3 cut(s) | 644, 1448, 2065 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 711, 1140 |
| BstYI | RGATCY | 3 cut(s) | 644, 1448, 2065 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1526 |
| BsuRI | GGCC | 4 cut(s) | 1087, 1101, 1422, 1735 |
| BtgI | CCRYGG | 1 cut(s) | 1427 |
| BtgZI | GCGATG | 1 cut(s) | 1184 |
| BtsCI | GGATG | 8 cut(s) | 132, 412, 727, 1438, 1477, 1822, 1826, 1883 |
| BtsI | GCAGTG | 2 cut(s) | 1369, 2122 |
| BtsIMutI | CAGTG | 7 cut(s) | 55, 470, 1038, 1369, 1522, 1801, 2122 |
| BveI | ACCTGC | 1 cut(s) | 320 |
| Cac8I | GCNNGC | 1 cut(s) | 1371 |
| CaiI | CAGNNNCTG | 3 cut(s) | 932, 1597, 2121 |
| CciI | TCATGA | 1 cut(s) | 2303 |
| Cfr13I | GGNCC | 6 cut(s) | 857, 971, 1085, 1421, 1880, 2241 |
| Csp6I | GTAC | 7 cut(s) | 435, 767, 952, 1072, 1095, 1651, 1942 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 509, 544 |
| CviQI | GTAC | 7 cut(s) | 435, 767, 952, 1072, 1095, 1651, 1942 |
| DdeI | CTNAG | 8 cut(s) | 47, 212, 402, 853, 1256, 1526, 1937, 2163 |
| Eam1104I | CTCTTC | 1 cut(s) | 1484 |
| EarI | CTCTTC | 1 cut(s) | 1484 |
| Ecl136II | GAGCTC | 2 cut(s) | 57, 357 |
| Eco130I | CCWWGG | 1 cut(s) | 1427 |
| Eco24I | GRGCYC | 2 cut(s) | 59, 359 |
| Eco47I | GGWCC | 4 cut(s) | 857, 971, 1880, 2241 |
| Eco53kI | GAGCTC | 2 cut(s) | 57, 357 |
| Eco57I | CTGAAG | 3 cut(s) | 954, 1493, 2028 |
| Eco81I | CCTNAGG | 1 cut(s) | 1526 |
| Eco88I | CYCGRG | 2 cut(s) | 1393, 2129 |
| EcoICRI | GAGCTC | 2 cut(s) | 57, 357 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 837 |
| EcoO109I | RGGNCCY | 2 cut(s) | 971, 1421 |
| EcoRI | GAATTC | 1 cut(s) | 786 |
| EcoRII | CCWGG | 6 cut(s) | 256, 832, 1081, 1717, 1876, 2081 |
| EcoT14I | CCWWGG | 1 cut(s) | 1427 |
| EcoT22I | ATGCAT | 2 cut(s) | 729, 1176 |
| EcoT38I | GRGCYC | 2 cut(s) | 59, 359 |
| ErhI | CCWWGG | 1 cut(s) | 1427 |
| Esp3I | CGTCTC | 1 cut(s) | 502 |
| FaqI | GGGAC | 3 cut(s) | 211, 1800, 2227 |
| FauNDI | CATATG | 3 cut(s) | 160, 604, 723 |
| FbaI | TGATCA | 1 cut(s) | 1588 |
| FblI | GTMKAC | 1 cut(s) | 1639 |
| Fnu4HI | GCNGC | 2 cut(s) | 273, 288 |
| FokI | GGATG | 8 cut(s) | 119, 419, 714, 1445, 1484, 1829, 1833, 1870 |
| FriOI | GRGCYC | 2 cut(s) | 59, 359 |
| Fsp4HI | GCNGC | 2 cut(s) | 273, 288 |
| FspBI | CTAG | 9 cut(s) | 291, 800, 917, 1103, 1370, 1466, 1790, 1889, 2076 |
| GluI | GCNGC | 2 cut(s) | 273, 288 |
| GsuI | CTGGAG | 2 cut(s) | 373, 1860 |
| HaeIII | GGCC | 4 cut(s) | 1087, 1101, 1422, 1735 |
| HapII | CCGG | 2 cut(s) | 642, 1442 |
| HincII | GTYRAC | 4 cut(s) | 497, 898, 1324, 1640 |
| HindII | GTYRAC | 4 cut(s) | 497, 898, 1324, 1640 |
| HindIII | AAGCTT | 1 cut(s) | 1309 |
| HinfI | GANTC | 5 cut(s) | 17, 199, 700, 2168, 2247 |
| HpaII | CCGG | 2 cut(s) | 642, 1442 |
| HphI | GGTGA | 3 cut(s) | 344, 727, 857 |
| Hpy166II | GTNNAC | 5 cut(s) | 497, 767, 898, 1324, 1640 |
| Hpy188I | TCNGA | 4 cut(s) | 1117, 1563, 1938, 1953 |
| Hpy8I | GTNNAC | 5 cut(s) | 497, 767, 898, 1324, 1640 |
| Hpy99I | CGWCG | 1 cut(s) | 2217 |
| HpyAV | CCTTC | 7 cut(s) | 107, 929, 1398, 1886, 2021, 2080, 2216 |
| HpyCH4III | ACNGT | 7 cut(s) | 474, 895, 1033, 1599, 1644, 1905, 1976 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 269, 278, 287, 407, 746, 1497, 1741, 2121 |
| HpyF3I | CTNAG | 8 cut(s) | 47, 212, 402, 853, 1256, 1526, 1937, 2163 |
| Ksp22I | TGATCA | 1 cut(s) | 1588 |
| LmnI | GCTCC | 1 cut(s) | 354 |
| Lsp1109I | GCAGC | 2 cut(s) | 274, 284 |
| MaeI | CTAG | 9 cut(s) | 291, 800, 917, 1103, 1370, 1466, 1790, 1889, 2076 |
| MaeIII | GTNAC | 3 cut(s) | 1660, 1858, 2117 |
| MfeI | CAATTG | 1 cut(s) | 2001 |
| MflI | RGATCY | 3 cut(s) | 644, 1448, 2065 |
| MhlI | GDGCHC | 3 cut(s) | 59, 359, 1269 |
| MlyI | GAGTC | 2 cut(s) | 193, 709 |
| MmeI | TCCRAC | 3 cut(s) | 920, 1396, 2285 |
| Mph1103I | ATGCAT | 2 cut(s) | 729, 1176 |
| MseI | TTAA | 5 cut(s) | 141, 416, 1076, 1146, 1281 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1806, 1963 |
| MspI | CCGG | 2 cut(s) | 642, 1442 |
| MspR9I | CCNGG | 7 cut(s) | 258, 834, 1083, 1443, 1719, 1878, 2083 |
| MunI | CAATTG | 1 cut(s) | 2001 |
| Mva1269I | GAATGC | 2 cut(s) | 949, 1813 |
| MvaI | CCWGG | 6 cut(s) | 258, 834, 1083, 1719, 1878, 2083 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 269, 278, 287, 407, 746, 1497, 1741, 2121 |
| NciI | CCSGG | 1 cut(s) | 1443 |
| NcoI | CCATGG | 1 cut(s) | 1427 |
| NdeI | CATATG | 3 cut(s) | 160, 604, 723 |
| NheI | GCTAGC | 1 cut(s) | 1369 |
| NlaIV | GGNNCC | 3 cut(s) | 973, 2139, 2243 |
| NmeAIII | GCCGAG | 1 cut(s) | 1672 |
| NmuCI | GTSAC | 3 cut(s) | 1660, 1858, 2117 |
| NsiI | ATGCAT | 2 cut(s) | 729, 1176 |
| NspI | RCATGY | 1 cut(s) | 740 |
| PaeR7I | CTCGAG | 1 cut(s) | 1393 |
| PagI | TCATGA | 1 cut(s) | 2303 |
| PaqCI | CACCTGC | 1 cut(s) | 320 |
| PasI | CCCWGGG | 1 cut(s) | 1082 |
| PctI | GAATGC | 2 cut(s) | 949, 1813 |
| PfeI | GAWTC | 3 cut(s) | 17, 2168, 2247 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 428 |
| PfoI | TCCNGGA | 1 cut(s) | 2081 |
| PkrI | GCNGC | 2 cut(s) | 274, 289 |
| PleI | GAGTC | 2 cut(s) | 193, 708 |
| PpsI | GAGTC | 2 cut(s) | 193, 708 |
| PpuMI | RGGWCCY | 1 cut(s) | 971 |
| PsiI | TTATAA | 2 cut(s) | 1199, 1346 |
| Psp124BI | GAGCTC | 2 cut(s) | 59, 359 |
| Psp5II | RGGWCCY | 1 cut(s) | 971 |
| Psp6I | CCWGG | 6 cut(s) | 256, 832, 1081, 1717, 1876, 2081 |
| PspGI | CCWGG | 6 cut(s) | 256, 832, 1081, 1717, 1876, 2081 |
| PspN4I | GGNNCC | 3 cut(s) | 973, 2139, 2243 |
| PspPI | GGNCC | 6 cut(s) | 857, 971, 1085, 1421, 1880, 2241 |
| PspPPI | RGGWCCY | 1 cut(s) | 971 |
| PspXI | VCTCGAGB | 1 cut(s) | 1393 |
| PsrI | GAACNNNNNNTAC | 4 cut(s) | 813, 845, 1113, 1145 |
| PstI | CTGCAG | 1 cut(s) | 331 |
| PstNI | CAGNNNCTG | 3 cut(s) | 932, 1597, 2121 |
| PsuI | RGATCY | 3 cut(s) | 644, 1448, 2065 |
| RsaI | GTAC | 7 cut(s) | 436, 768, 953, 1073, 1096, 1652, 1943 |
| RsaNI | GTAC | 7 cut(s) | 435, 767, 952, 1072, 1095, 1651, 1942 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1806, 1963 |
| SacI | GAGCTC | 2 cut(s) | 59, 359 |
| SalI | GTCGAC | 1 cut(s) | 1638 |
| SaqAI | TTAA | 5 cut(s) | 141, 416, 1076, 1146, 1281 |
| SatI | GCNGC | 2 cut(s) | 273, 288 |
| Sau96I | GGNCC | 6 cut(s) | 857, 971, 1085, 1421, 1880, 2241 |
| ScaI | AGTACT | 2 cut(s) | 953, 1073 |
| SchI | GAGTC | 2 cut(s) | 193, 709 |
| ScrFI | CCNGG | 7 cut(s) | 258, 834, 1083, 1443, 1719, 1878, 2083 |
| SduI | GDGCHC | 3 cut(s) | 59, 359, 1269 |
| SfcI | CTRYAG | 2 cut(s) | 327, 750 |
| Sfr274I | CTCGAG | 1 cut(s) | 1393 |
| SinI | GGWCC | 4 cut(s) | 857, 971, 1880, 2241 |
| SlaI | CTCGAG | 1 cut(s) | 1393 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1806, 1963 |
| SmlI | CTYRAG | 2 cut(s) | 687, 1393 |
| SmoI | CTYRAG | 2 cut(s) | 687, 1393 |
| SpeI | ACTAGT | 1 cut(s) | 1789 |
| SspI | AATATT | 1 cut(s) | 876 |
| SspMI | CTAG | 9 cut(s) | 291, 800, 917, 1103, 1370, 1466, 1790, 1889, 2076 |
| SstI | GAGCTC | 2 cut(s) | 59, 359 |
| StyD4I | CCNGG | 7 cut(s) | 256, 832, 1081, 1441, 1717, 1876, 2081 |
| StyI | CCWWGG | 1 cut(s) | 1427 |
| TaaI | ACNGT | 7 cut(s) | 474, 895, 1033, 1599, 1644, 1905, 1976 |
| TaqI | TCGA | 3 cut(s) | 1394, 1639, 2212 |
| TaqII | GACCGA | 1 cut(s) | 83 |
| TatI | WGTACW | 5 cut(s) | 434, 766, 951, 1071, 1650 |
| TfiI | GAWTC | 3 cut(s) | 17, 2168, 2247 |
| Tru1I | TTAA | 5 cut(s) | 141, 416, 1076, 1146, 1281 |
| Tru9I | TTAA | 5 cut(s) | 141, 416, 1076, 1146, 1281 |
| TscAI | CASTG | 7 cut(s) | 55, 477, 1038, 1369, 1522, 1808, 2122 |
| TseFI | GTSAC | 3 cut(s) | 1660, 1858, 2117 |
| TseI | GCWGC | 2 cut(s) | 272, 287 |
| Tsp45I | GTSAC | 3 cut(s) | 1660, 1858, 2117 |
| TspDTI | ATGAA | 7 cut(s) | 48, 357, 621, 1469, 1749, 1756, 1866 |
| TspRI | CASTG | 7 cut(s) | 55, 477, 1038, 1369, 1522, 1808, 2122 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 428 |
| VpaK11BI | GGWCC | 4 cut(s) | 857, 971, 1880, 2241 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 837 |
| XapI | RAATTY | 5 cut(s) | 771, 786, 1153, 1996, 2208 |
| XceI | RCATGY | 1 cut(s) | 740 |
| XhoI | CTCGAG | 1 cut(s) | 1393 |
| XmiI | GTMKAC | 1 cut(s) | 1639 |
| XspI | CTAG | 9 cut(s) | 291, 800, 917, 1103, 1370, 1466, 1790, 1889, 2076 |
| ZrmI | AGTACT | 2 cut(s) | 953, 1073 |
| Zsp2I | ATGCAT | 2 cut(s) | 729, 1176 |