MD03G1172000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0015579
Physical Location & Seq
Reverse (-)
23398923 .. 23405225
6303 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1172000.v1.1.491

Sequence Viewer

Length: 2943 bp
ATGAAAAGCCACCACAAAAGATTCAAGCCATCAAAACCTCTCTCTCTCTCTACCCTTTTAAAAGGTTCTGTGTCAGGGCCACCAAACATGTCCCTATTTTTCATCTGGGTCCTAGCGACTCTGAGATTTTTGGTGGCTGATGGTTCTGCTTCTTCATGGTGCCAGCAGCACCACCAACACTTTCTTGCACAGAGCAGCAGACAATTTGAGCAGAAAACAGACAGATTCTGGGAGTTTAAGGAGCAAACCAAAACATGGGTTGAAGTTGAATTGCCCTATGATTTGGTCTCCTGTCTCGATGACAACTGTACTGTTGTGGGTTCAATCGAGGGAACAGGAACATCATCAAACAACAGCAAACAGGAACAGAGTCCAGATTCTGATGATGTTCCAGGAAAAAAGGAAGAGGAGAGATCAGAAAAGAAGGACAAGGAGAATTGTGATCATAAGTTGCTTGAGGTCTTGCCTCAGAGAAAGAGGATTTCATTGACAAGAATGTCAGAAATGTCAATCTGGGTGACTGGGGCAAGTGGGTCAATCTATGAGAGGTTTTGGAATGGGGTCCAGTGGGTGATTGCACCCCATGATTTGCCTTTATCACCTGCTCATGCTATCTCTCTTTTTATTGTCAATCACACCATTCTTGCTCTTTCTGAATCAGGCAATCTGTTTCAGATGCAAATCAGCGAGAGCTCACACCCTCTTTGGGTAGAATTTACACCTACACTCAGTCAAAACGAAGGAGATCAAAGTTCTGTGATCCTAATTAAATCTGGTCTGGTTTCATATGATGCAGAGAGAGTCTATTTCTGCACAAAAAAAGGGACACTGTTAGAACTTAGTGAAATTGAGCCTCCAAGATGGATAAATCATGGACAACCCCCTGGCGCGAATGTTGCAGCAGTTGCCGATGCTGCTAGTATTAGAACAGATGTAATTTATACCATAAGTTCTGCAGGTGATCTTTATGAATATGATAGGAGCTCAAAACCATCATGGAAGAAGCATATATGGAGAGAAGGGAAAGCTAATTATAATGCTTCTCTAATACCGTTGACGGGGAATACTTTACATGGTGTGACTGGAGATCATTCTATATCCCTCTTTCTTCTAACAAAGGATGGTAAACTGGTCGAGAGACGACTACACCAGAGGAAATGGAAATGGGTAGTTTATGGAAGTCCAAAGGATCAGCGGCTGACATCAATCACACCAGTGCTGCAAGATGACACAAACGAAAGATTATTCTCGTTGTTTTTCACCACATCTACCGGATCAGTTTTCGAATATCAAATATCAAAACAATCAGGTATTGCTCAAGCTCAAGAGAACCAAGTTTTGGAAGCATGGGTAAGCCATATGCATCCTCTTCATGCAAAAGTGGCCAGAGGTATTGCTGGACTGCAAATTCATGTTGGTAGGATTCTGTTCCCATTGGATGATGGAAGACTTGCAGAACTGCATCTATCAGGCTTAGGTGGCGAAAACTCTGGTCCATCTCATCAAGTAATGCTCCGTAGAAAAGCAACAGTCAAATATGTGTGGTCAATAGTAGATGCGCCGGAGACGGAAGGCTGGAATGCAGAGTACTGCTCAGAACAGCGTGGACCCACAAATTGCGTCACGGGCATAAAAGATGAGCCTAATGATTTAGGAATTGGAAGAACAATGACAAGAAGGAGAAAAGGAAGCCAAGCACAGCAACATTACTTAACTCCAGGGGCATCAGGTAGCGGAACAATAAAATTTTCAGAAGATAATAGTTTCCCATACAACTGGATTAACACAAATTTCCATCTGAGAGCGATGCATGGAGGCAGATCATTTTTCCTCATAACAGATGGTGGTTTTACTTTCGAATATCTTTATACTGAAAGTGTATGGATATGGCTCAGGCATGAGCACTCAACAGCTATCAAAGGCGCACTAGGGAACTACAACGGAAGCTTGTATGTAGTTGACACATATGGGAGTTTGCTTATTAGGGAAAGAAGCAGCAATGATCTAGCATGGATAAACTGTACTGCACTGAGGAAAGGAAGGCAAGTAATCGGAGGCCCTCCATGGGATGGAATTCCTGGCAGAACTATGAAAGTTACAGCAGAAGACGCCCTCTTCTTTGTGAGCAGAACAGGCAGATTACTCCAGTTCACGGTTGCCCTGAGGAAATTCAAATGGAAAGATTGCCGAAACCCTCCAAATACGAAGATTGCTTCTATAGTAGATCAGGAACATTTCAGGGAAAACATAGTGTTTGTTGTTGGAAGGAATGGTCGGCTATATCAGTATAATAAAGTGACTGATTTGTGGCATGAGCATTACCAATCTCAGCATTTGACTTTATCAAGACTGCCTGGAACTGCTATGAGACCATCATCGCTTTCACTAACAGGATCACTTTTCATGCTTTCAGCAGACGGAGGACTAGTTGAATATCATTGGAATACATTCGAAGGATGGAATTGGGTGGAACACGGAAGTCCACATAGAGTTGTGACATTGGTTGGTTCTCCTGGTCCGTCCTTCGAAGGTAATCAACTGTTTCTTATTGGCTCCAATGGAAATGTTTACCTGAGGTACATGGAAGAAATGACATGGAGGTGGAAAAACTGTGGTTTTCCGTTTTTGGGAAAGTTGATTGTTGAAGATCGTAGACAAGAGGAAGGAAATGACAAGAAAGAATATTTCTGTACTGACGTAGATTTTGCAGCTAGTTCAAAGACGGATTATGAAAGAGATAATGACCTAAGCAGTGACTGTAACCCTGAGGTGGCGCCAATACGACCAATTCCCTTTGCTCAAGATTCAGTAATCTTTGAGCTACGAGATGGCAGATTGGCAGAAATGCGCCGGATAGAAGGCGGGCATTGGATATGGTCCCGAATCATCGCCACTCCGACAAGCTTATGCACAGCAAGTTACTGGACTGCTTTGGCATCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

981

Amino Acids

110.8

Weight (kDa)

7.07

Isoelectric Point (pI)

41.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015579)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g28450 FvH4_3g28450
malus_domestica MD03G1172000.v1.1
prunus_persica Prupe.4G254900_v2.0.a1 Prupe.4G254900_v2.0.a1
pyrus_communis pycom03g12910
rosa_chinensis RchiOBHm_Chr5g0053011
rosa_laevigata RLG00000034778
rosa_multiflora Rmu_sc0006573.1_g000003
rosa_roxburghii Rroxscaffold_1G00028830
rosa_rugosa Rorug05G0268800
rosa_samantha Rh5AG341100 Rh5BG351600 Rh5CG378000 Rh5DG368000
rosa_wichuraiana Rw5G032150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1035
AarI CACCTGC 2 cut(s) 610, 947
AasI GACNNNNNNGTC 1 cut(s) 496
Acc36I ACCTGC 2 cut(s) 610, 947
AccB1I GGYRCC 2 cut(s) 159, 2773
AccB7I CCANNNNNTGG 3 cut(s) 255, 1339, 2069
AccI GTMKAC 1 cut(s) 2653
AccII CGCG 1 cut(s) 890
AciI CCGC 3 cut(s) 1195, 1734, 2862
AclWI GGATC 4 cut(s) 754, 1197, 1282, 2401
AcoI YGGCCR 1 cut(s) 1383
AcsI RAATTY 6 cut(s) 713, 1407, 1745, 1789, 2073, 2168
AcyI GRCGYC 2 cut(s) 2109, 2774
AfaI GTAC 5 cut(s) 310, 1589, 2023, 2579, 2692
AfiI CCNNNNNNNGG 9 cut(s) 255, 706, 1058, 1339, 2065, 2069, 2152, 2627, 2770
AflIII ACRYGT 1 cut(s) 87
AgsI TTSAA 8 cut(s) 25, 263, 269, 324, 2173, 2432, 2645, 2718
AhlI ACTAGT 1 cut(s) 2425
AjnI CCWGG 6 cut(s) 391, 883, 1717, 2077, 2353, 2512
AjuI GAANNNNNNNTTGG 2 cut(s) 1322, 1354
AleI CACNNNNGTG 1 cut(s) 1214
AluBI AGCT 9 cut(s) 693, 984, 1028, 1322, 1913, 1947, 2711, 2821, 2904
AluI AGCT 9 cut(s) 693, 984, 1028, 1322, 1913, 1947, 2711, 2821, 2904
Alw21I GWGCWC 3 cut(s) 695, 986, 1905
Alw26I GTCTC 5 cut(s) 292, 299, 1132, 1559, 2362
AlwI GGATC 4 cut(s) 754, 1197, 1282, 2401
AlwNI CAGNNNCTG 4 cut(s) 228, 380, 1198, 2757
AoxI GGCC 3 cut(s) 77, 1383, 2056
ApeKI GCWGC 7 cut(s) 166, 195, 899, 914, 1219, 1995, 2708
ApoI RAATTY 6 cut(s) 713, 1407, 1745, 1789, 2073, 2168
ArsI GACNNNNNNTTYG 2 cut(s) 2687, 2719
Asp700I GAANNNNTTC 1 cut(s) 2447
AspLEI GCGC 5 cut(s) 890, 1561, 1925, 2776, 2850
AspS9I GGNCC 8 cut(s) 77, 109, 562, 1493, 1607, 2057, 2516, 2877
AsuHPI GGTGA 5 cut(s) 529, 583, 591, 971, 1252
AsuII TTCGAA 4 cut(s) 1284, 1857, 2451, 2526
AvaII GGWCC 6 cut(s) 109, 562, 1493, 1607, 2516, 2877
AxyI CCTNAGG 3 cut(s) 2162, 2573, 2766
BalI TGGCCA 1 cut(s) 1385
BanI GGYRCC 2 cut(s) 159, 2773
BanII GRGCYC 2 cut(s) 695, 986
BbsI GAAGAC 2 cut(s) 1453, 2112
Bbv12I GWGCWC 3 cut(s) 695, 986, 1905
BbvI GCAGC 7 cut(s) 178, 207, 901, 911, 1206, 2007, 2720
BciT130I CCWGG 6 cut(s) 393, 885, 1719, 2079, 2355, 2514
BclI TGATCA 1 cut(s) 442
BcoDI GTCTC 5 cut(s) 292, 299, 1132, 1559, 2362
BcuI ACTAGT 1 cut(s) 2425
BfaI CTAG 6 cut(s) 113, 918, 1928, 2006, 2426, 2712
BfmI CTRYAG 2 cut(s) 954, 2217
BfoI RGCGCY 1 cut(s) 2777
BfuAI ACCTGC 2 cut(s) 610, 947
BisI GCNGC 8 cut(s) 167, 196, 900, 915, 1196, 1220, 1996, 2709
BlsI GCNGC 8 cut(s) 168, 197, 901, 916, 1197, 1221, 1997, 2710
BmcAI AGTACT 1 cut(s) 1589
Bme1390I CCNGG 6 cut(s) 393, 885, 1719, 2079, 2355, 2514
Bme18I GGWCC 6 cut(s) 109, 562, 1493, 1607, 2516, 2877
BmgT120I GGNCC 8 cut(s) 77, 109, 562, 1493, 1607, 2057, 2516, 2877
BmiI GGNNCC 7 cut(s) 110, 161, 563, 1609, 2554, 2775, 2879
BmrFI CCNGG 6 cut(s) 393, 885, 1719, 2079, 2355, 2514
BmrI ACTGGG 1 cut(s) 531
BmsI GCATC 8 cut(s) 666, 781, 901, 1372, 1471, 1546, 1733, 1797
BmuI ACTGGG 1 cut(s) 531
BpiI GAAGAC 2 cut(s) 1453, 2112
BplI GAGNNNNNCTC 2 cut(s) 1577, 1609
BpmI CTGGAG 3 cut(s) 1104, 1701, 2129
Bpu10I CCTNAGC 3 cut(s) 1474, 1892, 2747
Bpu14I TTCGAA 4 cut(s) 1284, 1857, 2451, 2526
BpuEI CTTGAG 4 cut(s) 476, 1302, 1308, 2784
BsaBI GATNNNNATC 1 cut(s) 680
BsaHI GRCGYC 2 cut(s) 2109, 2774
BsaI GGTCTC 2 cut(s) 292, 2362
BsaJI CCNNGG 3 cut(s) 883, 1718, 2063
BsaWI WCCGGW 1 cut(s) 1271
BsaXI ACNNNNNCTCC 2 cut(s) 2412, 2442
Bsc4I CCNNNNNNNGG 9 cut(s) 255, 706, 1058, 1339, 2065, 2069, 2152, 2627, 2770
Bse1I ACTGG 8 cut(s) 526, 565, 1087, 1134, 1214, 1781, 2146, 2927
Bse21I CCTNAGG 3 cut(s) 2162, 2573, 2766
Bse3DI GCAATG 1 cut(s) 2005
Bse8I GATNNNNATC 1 cut(s) 680
BseBI CCWGG 6 cut(s) 393, 885, 1719, 2079, 2355, 2514
BseDI CCNNGG 3 cut(s) 883, 1718, 2063
BseGI GGATG 5 cut(s) 1126, 1363, 1444, 2074, 2462
BseJI GATNNNNATC 1 cut(s) 680
BseLI CCNNNNNNNGG 9 cut(s) 255, 706, 1058, 1339, 2065, 2069, 2152, 2627, 2770
BseMI GCAATG 1 cut(s) 2005
BseNI ACTGG 8 cut(s) 526, 565, 1087, 1134, 1214, 1781, 2146, 2927
BseRI GAGGAG 1 cut(s) 422
BseXI GCAGC 7 cut(s) 178, 207, 901, 911, 1206, 2007, 2720
BsgI GTGCAG 2 cut(s) 796, 2010
Bsh1236I CGCG 1 cut(s) 890
BshFI GGCC 3 cut(s) 79, 1385, 2058
BshNI GGYRCC 2 cut(s) 159, 2773
BsiHKAI GWGCWC 3 cut(s) 695, 986, 1905
BsiSI CCGG 3 cut(s) 1272, 1562, 2851
BslFI GGGAC 3 cut(s) 76, 838, 2863
BslI CCNNNNNNNGG 9 cut(s) 255, 706, 1058, 1339, 2065, 2069, 2152, 2627, 2770
BsmAI GTCTC 5 cut(s) 292, 299, 1132, 1559, 2362
BsmBI CGTCTC 2 cut(s) 1132, 1559
BsmFI GGGAC 3 cut(s) 76, 838, 2863
BsmI GAATGC 1 cut(s) 1585
BsnI GGCC 3 cut(s) 79, 1385, 2058
Bso31I GGTCTC 2 cut(s) 292, 2362
Bsp119I TTCGAA 4 cut(s) 1284, 1857, 2451, 2526
Bsp1286I GDGCHC 3 cut(s) 695, 986, 1905
Bsp19I CCATGG 1 cut(s) 2063
BspACI CCGC 3 cut(s) 1195, 1734, 2862
BspANI GGCC 3 cut(s) 79, 1385, 2058
BspFNI CGCG 1 cut(s) 890
BspLI GGNNCC 7 cut(s) 110, 161, 563, 1609, 2554, 2775, 2879
BspMAI CTGCAG 1 cut(s) 958
BspMI ACCTGC 2 cut(s) 610, 947
BspPI GGATC 4 cut(s) 754, 1197, 1282, 2401
BspT104I TTCGAA 4 cut(s) 1284, 1857, 2451, 2526
BspT107I GGYRCC 2 cut(s) 159, 2773
BspTNI GGTCTC 2 cut(s) 292, 2362
BsrDI GCAATG 1 cut(s) 2005
BsrI ACTGG 8 cut(s) 526, 565, 1087, 1134, 1214, 1781, 2146, 2927
BssECI CCNNGG 3 cut(s) 883, 1718, 2063
BssNI GRCGYC 2 cut(s) 2109, 2774
BssT1I CCWWGG 1 cut(s) 2063
Bst2UI CCWGG 6 cut(s) 393, 885, 1719, 2079, 2355, 2514
Bst6I CTCTTC 3 cut(s) 399, 1374, 2120
BstACI GRCGYC 2 cut(s) 2109, 2774
BstAPI GCANNNNNTGC 1 cut(s) 905
BstBI TTCGAA 4 cut(s) 1284, 1857, 2451, 2526
BstC8I GCNNGC 2 cut(s) 164, 2864
BstDSI CCRYGG 1 cut(s) 2063
BstF5I GGATG 5 cut(s) 1126, 1363, 1444, 2074, 2462
BstFNI CGCG 1 cut(s) 890
BstH2I RGCGCY 1 cut(s) 2777
BstHHI GCGC 5 cut(s) 890, 1561, 1925, 2776, 2850
BstMAI GTCTC 5 cut(s) 292, 299, 1132, 1559, 2362
BstMWI GCNNNNNNNGC 8 cut(s) 896, 905, 914, 1382, 1479, 1626, 2108, 2133
BstNI CCWGG 6 cut(s) 393, 885, 1719, 2079, 2355, 2514
BstNSI RCATGY 1 cut(s) 91
BstSCI CCNGG 6 cut(s) 391, 883, 1717, 2077, 2353, 2512
BstSFI CTRYAG 2 cut(s) 954, 2217
BstUI CGCG 1 cut(s) 890
BstV1I GCAGC 7 cut(s) 178, 207, 901, 911, 1206, 2007, 2720
BstV2I GAAGAC 2 cut(s) 1453, 2112
BstXI CCANNNNNNTGG 1 cut(s) 1776
Bsu36I CCTNAGG 3 cut(s) 2162, 2573, 2766
BsuRI GGCC 3 cut(s) 79, 1385, 2058
BtgI CCRYGG 1 cut(s) 2063
BtgZI GCGATG 3 cut(s) 1820, 2361, 2872
BtsCI GGATG 5 cut(s) 1126, 1363, 1444, 2074, 2462
BtsI GCAGTG 1 cut(s) 2758
BtsIMutI CAGTG 5 cut(s) 572, 827, 1221, 2027, 2758
BveI ACCTGC 2 cut(s) 610, 947
Cac8I GCNNGC 2 cut(s) 164, 2864
CaiI CAGNNNCTG 4 cut(s) 228, 380, 1198, 2757
CfoI GCGC 5 cut(s) 890, 1561, 1925, 2776, 2850
Cfr13I GGNCC 8 cut(s) 77, 109, 562, 1493, 1607, 2057, 2516, 2877
CseI GACGC 2 cut(s) 1609, 2117
Csp6I GTAC 5 cut(s) 309, 1588, 2022, 2578, 2691
CviQI GTAC 5 cut(s) 309, 1588, 2022, 2578, 2691
DinI GGCGCC 1 cut(s) 2775
DraI TTTAAA 1 cut(s) 60
DrdI GACNNNNNNGTC 1 cut(s) 496
DseDI GACNNNNNNGTC 1 cut(s) 496
EaeI YGGCCR 1 cut(s) 1383
Eam1104I CTCTTC 3 cut(s) 399, 1374, 2120
EarI CTCTTC 3 cut(s) 399, 1374, 2120
Ecl136II GAGCTC 2 cut(s) 693, 984
Eco130I CCWWGG 1 cut(s) 2063
Eco24I GRGCYC 2 cut(s) 695, 986
Eco31I GGTCTC 2 cut(s) 292, 2362
Eco47I GGWCC 6 cut(s) 109, 562, 1493, 1607, 2516, 2877
Eco53kI GAGCTC 2 cut(s) 693, 984
Eco81I CCTNAGG 3 cut(s) 2162, 2573, 2766
EcoICRI GAGCTC 2 cut(s) 693, 984
EcoO109I RGGNCCY 2 cut(s) 109, 2057
EcoRI GAATTC 1 cut(s) 2073
EcoRII CCWGG 6 cut(s) 391, 883, 1717, 2077, 2353, 2512
EcoT14I CCWWGG 1 cut(s) 2063
EcoT22I ATGCAT 2 cut(s) 1365, 1812
EcoT38I GRGCYC 2 cut(s) 695, 986
EgeI GGCGCC 1 cut(s) 2775
EheI GGCGCC 1 cut(s) 2775
ErhI CCWWGG 1 cut(s) 2063
Esp3I CGTCTC 2 cut(s) 1132, 1559
FaqI GGGAC 3 cut(s) 76, 838, 2863
FauI CCCGC 1 cut(s) 2855
FauNDI CATATG 3 cut(s) 787, 1359, 1966
FbaI TGATCA 1 cut(s) 442
FblI GTMKAC 1 cut(s) 2653
Fnu4HI GCNGC 8 cut(s) 167, 196, 900, 915, 1196, 1220, 1996, 2709
FokI GGATG 5 cut(s) 1133, 1350, 1451, 2081, 2469
FriOI GRGCYC 2 cut(s) 695, 986
Fsp4HI GCNGC 8 cut(s) 167, 196, 900, 915, 1196, 1220, 1996, 2709
FspBI CTAG 6 cut(s) 113, 918, 1928, 2006, 2426, 2712
GlaI GCGC 5 cut(s) 889, 1560, 1924, 2775, 2849
GluI GCNGC 8 cut(s) 167, 196, 900, 915, 1196, 1220, 1996, 2709
GsuI CTGGAG 3 cut(s) 1104, 1701, 2129
HaeII RGCGCY 1 cut(s) 2777
HaeIII GGCC 3 cut(s) 79, 1385, 2058
HapII CCGG 3 cut(s) 1272, 1562, 2851
HgaI GACGC 2 cut(s) 1609, 2117
HhaI GCGC 5 cut(s) 890, 1561, 1925, 2776, 2850
Hin1I GRCGYC 2 cut(s) 2109, 2774
Hin6I GCGC 5 cut(s) 888, 1559, 1923, 2774, 2848
HinP1I GCGC 5 cut(s) 888, 1559, 1923, 2774, 2848
HincII GTYRAC 2 cut(s) 1056, 1960
HindII GTYRAC 2 cut(s) 1056, 1960
HindIII AAGCTT 2 cut(s) 1945, 2902
HpaII CCGG 3 cut(s) 1272, 1562, 2851
HphI GGTGA 5 cut(s) 529, 583, 591, 971, 1252
Hpy166II GTNNAC 8 cut(s) 1056, 1127, 1607, 1960, 2151, 2483, 2569, 2654
Hpy188III TCNNGA 9 cut(s) 296, 374, 1135, 1325, 2228, 2346, 2801, 2880, 2940
Hpy8I GTNNAC 8 cut(s) 1056, 1127, 1607, 1960, 2151, 2483, 2569, 2654
HpyCH4IV ACGT 1 cut(s) 2697
HpyF10VI GCNNNNNNNGC 8 cut(s) 896, 905, 914, 1382, 1479, 1626, 2108, 2133
HpySE526I ACGT 1 cut(s) 2697
Hsp92I GRCGYC 2 cut(s) 2109, 2774
HspAI GCGC 5 cut(s) 888, 1559, 1923, 2774, 2848
KasI GGCGCC 1 cut(s) 2773
Ksp22I TGATCA 1 cut(s) 442
LmnI GCTCC 4 cut(s) 241, 981, 1518, 2558
Lsp1109I GCAGC 7 cut(s) 178, 207, 901, 911, 1206, 2007, 2720
LweI GCATC 8 cut(s) 666, 781, 901, 1372, 1471, 1546, 1733, 1797
MaeI CTAG 6 cut(s) 113, 918, 1928, 2006, 2426, 2712
MaeII ACGT 1 cut(s) 2697
MaeIII GTNAC 9 cut(s) 517, 1078, 1621, 2095, 2296, 2494, 2753, 2759, 2918
MhlI GDGCHC 3 cut(s) 695, 986, 1905
MlsI TGGCCA 1 cut(s) 1385
MluNI TGGCCA 1 cut(s) 1385
Mly113I GGCGCC 1 cut(s) 2774
MlyI GAGTC 3 cut(s) 112, 379, 810
MmeI TCCRAC 2 cut(s) 2242, 2921
Mox20I TGGCCA 1 cut(s) 1385
Mph1103I ATGCAT 2 cut(s) 1365, 1812
MroXI GAANNNNTTC 1 cut(s) 2447
MscI TGGCCA 1 cut(s) 1385
MseI TTAA 5 cut(s) 59, 237, 768, 1712, 1782
MslI CAYNNNNRTG 2 cut(s) 1214, 2599
Msp20I TGGCCA 1 cut(s) 1385
MspA1I CMGCKG 1 cut(s) 1195
MspI CCGG 3 cut(s) 1272, 1562, 2851
MspR9I CCNGG 6 cut(s) 393, 885, 1719, 2079, 2355, 2514
Mva1269I GAATGC 1 cut(s) 1585
MvaI CCWGG 6 cut(s) 393, 885, 1719, 2079, 2355, 2514
MvnI CGCG 1 cut(s) 890
MwoI GCNNNNNNNGC 8 cut(s) 896, 905, 914, 1382, 1479, 1626, 2108, 2133
NarI GGCGCC 1 cut(s) 2774
NcoI CCATGG 1 cut(s) 2063
NdeI CATATG 3 cut(s) 787, 1359, 1966
NlaIV GGNNCC 7 cut(s) 110, 161, 563, 1609, 2554, 2775, 2879
NmuCI GTSAC 6 cut(s) 517, 1078, 1621, 2296, 2494, 2753
NsiI ATGCAT 2 cut(s) 1365, 1812
NspI RCATGY 1 cut(s) 91
NspV TTCGAA 4 cut(s) 1284, 1857, 2451, 2526
OliI CACNNNNGTG 1 cut(s) 1214
PaqCI CACCTGC 2 cut(s) 610, 947
PciI ACATGT 1 cut(s) 87
PctI GAATGC 1 cut(s) 1585
PdmI GAANNNNTTC 1 cut(s) 2447
PfeI GAWTC 7 cut(s) 21, 225, 377, 656, 1423, 2804, 2883
PflMI CCANNNNNTGG 3 cut(s) 255, 1339, 2069
PfoI TCCNGGA 1 cut(s) 391
PkrI GCNGC 8 cut(s) 168, 197, 901, 916, 1197, 1221, 1997, 2710
PleI GAGTC 3 cut(s) 112, 378, 809
PluTI GGCGCC 1 cut(s) 2777
PpsI GAGTC 3 cut(s) 112, 378, 809
PpuMI RGGWCCY 1 cut(s) 109
PscI ACATGT 1 cut(s) 87
PsiI TTATAA 1 cut(s) 1035
Psp124BI GAGCTC 2 cut(s) 695, 986
Psp5II RGGWCCY 1 cut(s) 109
Psp6I CCWGG 6 cut(s) 391, 883, 1717, 2077, 2353, 2512
PspGI CCWGG 6 cut(s) 391, 883, 1717, 2077, 2353, 2512
PspN4I GGNNCC 7 cut(s) 110, 161, 563, 1609, 2554, 2775, 2879
PspPI GGNCC 8 cut(s) 77, 109, 562, 1493, 1607, 2057, 2516, 2877
PspPPI RGGWCCY 1 cut(s) 109
PstI CTGCAG 1 cut(s) 958
PstNI CAGNNNCTG 4 cut(s) 228, 380, 1198, 2757
RsaI GTAC 5 cut(s) 310, 1589, 2023, 2579, 2692
RsaNI GTAC 5 cut(s) 309, 1588, 2022, 2578, 2691
RseI CAYNNNNRTG 2 cut(s) 1214, 2599
SacI GAGCTC 2 cut(s) 695, 986
SaqAI TTAA 5 cut(s) 59, 237, 768, 1712, 1782
SatI GCNGC 8 cut(s) 167, 196, 900, 915, 1196, 1220, 1996, 2709
Sau96I GGNCC 8 cut(s) 77, 109, 562, 1493, 1607, 2057, 2516, 2877
ScaI AGTACT 1 cut(s) 1589
SchI GAGTC 3 cut(s) 112, 379, 810
ScrFI CCNGG 6 cut(s) 393, 885, 1719, 2079, 2355, 2514
SduI GDGCHC 3 cut(s) 695, 986, 1905
SfaNI GCATC 8 cut(s) 666, 781, 901, 1372, 1471, 1546, 1733, 1797
SfcI CTRYAG 2 cut(s) 954, 2217
SfoI GGCGCC 1 cut(s) 2775
SfuI TTCGAA 4 cut(s) 1284, 1857, 2451, 2526
SinI GGWCC 6 cut(s) 109, 562, 1493, 1607, 2516, 2877
SmiMI CAYNNNNRTG 2 cut(s) 1214, 2599
SmlI CTYRAG 4 cut(s) 455, 1317, 1323, 2799
SmoI CTYRAG 4 cut(s) 455, 1317, 1323, 2799
SpeI ACTAGT 1 cut(s) 2425
SsiI CCGC 3 cut(s) 1195, 1734, 2862
SspDI GGCGCC 1 cut(s) 2773
SspI AATATT 1 cut(s) 2684
SspMI CTAG 6 cut(s) 113, 918, 1928, 2006, 2426, 2712
SstI GAGCTC 2 cut(s) 695, 986
StyD4I CCNGG 6 cut(s) 391, 883, 1717, 2077, 2353, 2512
StyI CCWWGG 1 cut(s) 2063
TaiI ACGT 1 cut(s) 2700
TaqI TCGA 7 cut(s) 297, 327, 1134, 1284, 1857, 2451, 2526
TatI WGTACW 4 cut(s) 308, 1587, 2021, 2690
TauI GCSGC 1 cut(s) 1198
TfiI GAWTC 7 cut(s) 21, 225, 377, 656, 1423, 2804, 2883
Tru1I TTAA 5 cut(s) 59, 237, 768, 1712, 1782
Tru9I TTAA 5 cut(s) 59, 237, 768, 1712, 1782
TscAI CASTG 5 cut(s) 572, 834, 1221, 2034, 2758
TseFI GTSAC 6 cut(s) 517, 1078, 1621, 2296, 2494, 2753
TseI GCWGC 7 cut(s) 166, 195, 899, 914, 1219, 1995, 2708
Tsp45I GTSAC 6 cut(s) 517, 1078, 1621, 2296, 2494, 2753
TspGWI ACGGA 8 cut(s) 1505, 1583, 1956, 2433, 2490, 2508, 2610, 2738
TspRI CASTG 5 cut(s) 572, 834, 1221, 2034, 2758
Van91I CCANNNNNTGG 3 cut(s) 255, 1339, 2069
VpaK11BI GGWCC 6 cut(s) 109, 562, 1493, 1607, 2516, 2877
XapI RAATTY 6 cut(s) 713, 1407, 1745, 1789, 2073, 2168
XceI RCATGY 1 cut(s) 91
XmiI GTMKAC 1 cut(s) 2653
XmnI GAANNNNTTC 1 cut(s) 2447
XspI CTAG 6 cut(s) 113, 918, 1928, 2006, 2426, 2712
ZrmI AGTACT 1 cut(s) 1589
Zsp2I ATGCAT 2 cut(s) 1365, 1812
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.