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| Species | Orthologous Gene IDs |
|---|---|
| fragaria_vesca | FvH4_3g28450 FvH4_3g28450 |
| malus_domestica | MD03G1172000.v1.1 |
| prunus_persica | Prupe.4G254900_v2.0.a1 Prupe.4G254900_v2.0.a1 |
| pyrus_communis | pycom03g12910 |
| rosa_chinensis | RchiOBHm_Chr5g0053011 |
| rosa_laevigata | RLG00000034778 |
| rosa_multiflora | Rmu_sc0006573.1_g000003 |
| rosa_roxburghii | Rroxscaffold_1G00028830 |
| rosa_rugosa | Rorug05G0268800 |
| rosa_samantha | Rh5AG341100 Rh5BG351600 Rh5CG378000 Rh5DG368000 |
| rosa_wichuraiana | Rw5G032150 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 860 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 400 |
| Acc36I | ACCTGC | 1 cut(s) | 860 |
| AccB1I | GGYRCC | 3 cut(s) | 81, 481, 2677 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 168, 1976 |
| AccI | GTMKAC | 2 cut(s) | 615, 2604 |
| AccII | CGCG | 2 cut(s) | 989, 2785 |
| AciI | CCGC | 3 cut(s) | 1433, 2390, 2520 |
| AclWI | GGATC | 4 cut(s) | 28, 41, 1107, 1195 |
| AcsI | RAATTY | 4 cut(s) | 384, 1314, 1696, 1980 |
| AcuI | CTGAAG | 1 cut(s) | 2724 |
| AcyI | GRCGYC | 1 cut(s) | 2016 |
| AfaI | GTAC | 4 cut(s) | 223, 976, 1058, 2596 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 168, 503, 968, 1976 |
| AhlI | ACTAGT | 1 cut(s) | 2332 |
| AjnI | CCWGG | 4 cut(s) | 796, 1624, 1984, 2260 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 78, 110 |
| AleI | CACNNNNGTG | 1 cut(s) | 1124 |
| Alw21I | GWGCWC | 7 cut(s) | 267, 511, 599, 899, 1812, 1834, 2226 |
| Alw26I | GTCTC | 2 cut(s) | 523, 1042 |
| Alw44I | GTGCAC | 1 cut(s) | 1830 |
| AlwI | GGATC | 4 cut(s) | 28, 41, 1107, 1195 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 521, 1108, 2140 |
| AoxI | GGCC | 1 cut(s) | 1642 |
| ApaLI | GTGCAC | 1 cut(s) | 1830 |
| ApeKI | GCWGC | 7 cut(s) | 809, 812, 827, 1129, 1132, 1609, 2612 |
| ApoI | RAATTY | 4 cut(s) | 384, 1314, 1696, 1980 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2591, 2623 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2354 |
| AspLEI | GCGC | 2 cut(s) | 989, 1107 |
| AspS9I | GGNCC | 6 cut(s) | 466, 1400, 1514, 1643, 1964, 2423 |
| AsuC2I | CCSGG | 1 cut(s) | 2421 |
| AsuHPI | GGTGA | 7 cut(s) | 440, 487, 884, 1165, 1400, 2409, 2576 |
| AsuII | TTCGAA | 1 cut(s) | 1197 |
| AvaII | GGWCC | 5 cut(s) | 466, 1400, 1514, 1964, 2423 |
| AxyI | CCTNAGG | 1 cut(s) | 2069 |
| BaeGI | GKGCMC | 1 cut(s) | 1834 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1040, 1073 |
| BamHI | GGATCC | 1 cut(s) | 33 |
| BanI | GGYRCC | 3 cut(s) | 81, 481, 2677 |
| BanII | GRGCYC | 3 cut(s) | 511, 599, 899 |
| BbsI | GAAGAC | 3 cut(s) | 134, 1360, 2019 |
| Bbv12I | GWGCWC | 7 cut(s) | 267, 511, 599, 899, 1812, 1834, 2226 |
| BbvCI | CCTCAGC | 1 cut(s) | 1799 |
| BbvI | GCAGC | 7 cut(s) | 796, 814, 824, 1116, 1119, 1621, 2624 |
| BciT130I | CCWGG | 4 cut(s) | 798, 1626, 1986, 2262 |
| BcnI | CCSGG | 1 cut(s) | 2421 |
| BcoDI | GTCTC | 2 cut(s) | 523, 1042 |
| BcuI | ACTAGT | 1 cut(s) | 2332 |
| BfaI | CTAG | 4 cut(s) | 1835, 1913, 2333, 2616 |
| BfmI | CTRYAG | 5 cut(s) | 225, 810, 867, 2051, 2124 |
| BfoI | RGCGCY | 1 cut(s) | 1108 |
| BfuAI | ACCTGC | 1 cut(s) | 860 |
| BisI | GCNGC | 8 cut(s) | 810, 813, 828, 1130, 1133, 1434, 1610, 2613 |
| BlsI | GCNGC | 8 cut(s) | 811, 814, 829, 1131, 1134, 1435, 1611, 2614 |
| BmcAI | AGTACT | 1 cut(s) | 976 |
| Bme1390I | CCNGG | 5 cut(s) | 798, 1626, 1986, 2262, 2421 |
| Bme18I | GGWCC | 5 cut(s) | 466, 1400, 1514, 1964, 2423 |
| BmgT120I | GGNCC | 6 cut(s) | 466, 1400, 1514, 1643, 1964, 2423 |
| BmiI | GGNNCC | 7 cut(s) | 35, 83, 467, 483, 1516, 2461, 2679 |
| BmrFI | CCNGG | 5 cut(s) | 798, 1626, 1986, 2262, 2421 |
| BmsI | GCATC | 5 cut(s) | 814, 937, 1453, 1536, 1704 |
| BpiI | GAAGAC | 3 cut(s) | 134, 1360, 2019 |
| BpmI | CTGGAG | 1 cut(s) | 1608 |
| Bpu10I | CCTNAGC | 3 cut(s) | 1381, 1799, 2144 |
| Bpu14I | TTCGAA | 1 cut(s) | 1197 |
| BpuEI | CTTGAG | 2 cut(s) | 1215, 1957 |
| BpuMI | CCSGG | 1 cut(s) | 2421 |
| BsaBI | GATNNNNATC | 2 cut(s) | 584, 2664 |
| BsaHI | GRCGYC | 1 cut(s) | 2016 |
| BsaJI | CCNNGG | 4 cut(s) | 796, 1970, 2697, 2835 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 699, 729, 2319, 2349 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 168, 503, 968, 1976 |
| Bse21I | CCTNAGG | 1 cut(s) | 2069 |
| Bse3DI | GCAATG | 2 cut(s) | 1912, 2282 |
| Bse8I | GATNNNNATC | 2 cut(s) | 584, 2664 |
| BseBI | CCWGG | 4 cut(s) | 798, 1626, 1986, 2262 |
| BseDI | CCNNGG | 4 cut(s) | 796, 1970, 2697, 2835 |
| BseJI | GATNNNNATC | 2 cut(s) | 584, 2664 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 168, 503, 968, 1976 |
| BseMI | GCAATG | 2 cut(s) | 1912, 2282 |
| BseMII | CTCAG | 7 cut(s) | 365, 646, 1790, 2060, 2158, 2249, 2471 |
| BseSI | GKGCMC | 1 cut(s) | 1834 |
| BseXI | GCAGC | 7 cut(s) | 796, 814, 824, 1116, 1119, 1621, 2624 |
| BseYI | CCCAGC | 1 cut(s) | 168 |
| BsgI | GTGCAG | 1 cut(s) | 709 |
| Bsh1236I | CGCG | 2 cut(s) | 989, 2785 |
| BshFI | GGCC | 1 cut(s) | 1644 |
| BshNI | GGYRCC | 3 cut(s) | 81, 481, 2677 |
| BsiHKAI | GWGCWC | 7 cut(s) | 267, 511, 599, 899, 1812, 1834, 2226 |
| BsiSI | CCGG | 1 cut(s) | 2421 |
| BslFI | GGGAC | 1 cut(s) | 751 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 168, 503, 968, 1976 |
| BsmAI | GTCTC | 2 cut(s) | 523, 1042 |
| BsmBI | CGTCTC | 1 cut(s) | 1042 |
| BsmFI | GGGAC | 1 cut(s) | 751 |
| BsmI | GAATGC | 1 cut(s) | 1492 |
| BsnI | GGCC | 1 cut(s) | 1644 |
| Bsp119I | TTCGAA | 1 cut(s) | 1197 |
| Bsp1286I | GDGCHC | 7 cut(s) | 267, 511, 599, 899, 1812, 1834, 2226 |
| Bsp19I | CCATGG | 1 cut(s) | 1970 |
| BspACI | CCGC | 3 cut(s) | 1433, 2390, 2520 |
| BspANI | GGCC | 1 cut(s) | 1644 |
| BspCNI | CTCAG | 7 cut(s) | 366, 645, 1791, 2061, 2157, 2248, 2472 |
| BspFNI | CGCG | 2 cut(s) | 989, 2785 |
| BspHI | TCATGA | 1 cut(s) | 2843 |
| BspLI | GGNNCC | 7 cut(s) | 35, 83, 467, 483, 1516, 2461, 2679 |
| BspMAI | CTGCAG | 2 cut(s) | 814, 871 |
| BspMI | ACCTGC | 1 cut(s) | 860 |
| BspPI | GGATC | 4 cut(s) | 28, 41, 1107, 1195 |
| BspQI | GCTCTTC | 1 cut(s) | 255 |
| BspT104I | TTCGAA | 1 cut(s) | 1197 |
| BspT107I | GGYRCC | 3 cut(s) | 81, 481, 2677 |
| BsrDI | GCAATG | 2 cut(s) | 1912, 2282 |
| BssECI | CCNNGG | 4 cut(s) | 796, 1970, 2697, 2835 |
| BssNI | GRCGYC | 1 cut(s) | 2016 |
| BssT1I | CCWWGG | 3 cut(s) | 1970, 2697, 2835 |
| Bst2UI | CCWGG | 4 cut(s) | 798, 1626, 1986, 2262 |
| Bst4CI | ACNGT | 9 cut(s) | 221, 226, 1056, 1670, 2055, 2062, 2214, 2448, 2657 |
| Bst6I | CTCTTC | 3 cut(s) | 255, 2027, 2554 |
| BstACI | GRCGYC | 1 cut(s) | 2016 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 809, 1289 |
| BstBI | TTCGAA | 1 cut(s) | 1197 |
| BstC8I | GCNNGC | 1 cut(s) | 2768 |
| BstDSI | CCRYGG | 1 cut(s) | 1970 |
| BstFNI | CGCG | 2 cut(s) | 989, 2785 |
| BstH2I | RGCGCY | 1 cut(s) | 1108 |
| BstHHI | GCGC | 2 cut(s) | 989, 1107 |
| BstMAI | GTCTC | 2 cut(s) | 523, 1042 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 809, 818, 827, 947, 1289, 1533, 2015, 2040 |
| BstNI | CCWGG | 4 cut(s) | 798, 1626, 1986, 2262 |
| BstNSI | RCATGY | 1 cut(s) | 1283 |
| BstSCI | CCNGG | 5 cut(s) | 796, 1624, 1984, 2260, 2419 |
| BstSFI | CTRYAG | 5 cut(s) | 225, 810, 867, 2051, 2124 |
| BstSLI | GKGCMC | 1 cut(s) | 1834 |
| BstUI | CGCG | 2 cut(s) | 989, 2785 |
| BstV1I | GCAGC | 7 cut(s) | 796, 814, 824, 1116, 1119, 1621, 2624 |
| BstV2I | GAAGAC | 3 cut(s) | 134, 1360, 2019 |
| BstX2I | RGATCY | 1 cut(s) | 33 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1254, 1683 |
| BstYI | RGATCY | 1 cut(s) | 33 |
| Bsu36I | CCTNAGG | 1 cut(s) | 2069 |
| BsuRI | GGCC | 1 cut(s) | 1644 |
| BtgI | CCRYGG | 1 cut(s) | 1970 |
| BtgZI | GCGATG | 1 cut(s) | 1727 |
| BtsI | GCAGTG | 2 cut(s) | 1885, 1929 |
| BtsIMutI | CAGTG | 9 cut(s) | 423, 476, 595, 1131, 1182, 1340, 1885, 1929, 2662 |
| BveI | ACCTGC | 1 cut(s) | 860 |
| Cac8I | GCNNGC | 1 cut(s) | 2768 |
| CaiI | CAGNNNCTG | 3 cut(s) | 521, 1108, 2140 |
| CciI | TCATGA | 1 cut(s) | 2843 |
| CfoI | GCGC | 2 cut(s) | 989, 1107 |
| Cfr13I | GGNCC | 6 cut(s) | 466, 1400, 1514, 1643, 1964, 2423 |
| CseI | GACGC | 2 cut(s) | 1877, 2024 |
| Csp6I | GTAC | 4 cut(s) | 222, 975, 1057, 2595 |
| CviQI | GTAC | 4 cut(s) | 222, 975, 1057, 2595 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 400 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 400 |
| Eam1104I | CTCTTC | 3 cut(s) | 255, 2027, 2554 |
| EarI | CTCTTC | 3 cut(s) | 255, 2027, 2554 |
| Ecl136II | GAGCTC | 3 cut(s) | 509, 597, 897 |
| Eco130I | CCWWGG | 3 cut(s) | 1970, 2697, 2835 |
| Eco24I | GRGCYC | 3 cut(s) | 511, 599, 899 |
| Eco47I | GGWCC | 5 cut(s) | 466, 1400, 1514, 1964, 2423 |
| Eco53kI | GAGCTC | 3 cut(s) | 509, 597, 897 |
| Eco57I | CTGAAG | 1 cut(s) | 2724 |
| Eco81I | CCTNAGG | 1 cut(s) | 2069 |
| EcoICRI | GAGCTC | 3 cut(s) | 509, 597, 897 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1964 |
| EcoRI | GAATTC | 1 cut(s) | 1980 |
| EcoRII | CCWGG | 4 cut(s) | 796, 1624, 1984, 2260 |
| EcoT14I | CCWWGG | 3 cut(s) | 1970, 2697, 2835 |
| EcoT22I | ATGCAT | 1 cut(s) | 1719 |
| EcoT38I | GRGCYC | 3 cut(s) | 511, 599, 899 |
| ErhI | CCWWGG | 3 cut(s) | 1970, 2697, 2835 |
| Esp3I | CGTCTC | 1 cut(s) | 1042 |
| FaqI | GGGAC | 1 cut(s) | 751 |
| FauNDI | CATATG | 1 cut(s) | 700 |
| FblI | GTMKAC | 2 cut(s) | 615, 2604 |
| Fnu4HI | GCNGC | 8 cut(s) | 810, 813, 828, 1130, 1133, 1434, 1610, 2613 |
| FriOI | GRGCYC | 3 cut(s) | 511, 599, 899 |
| Fsp4HI | GCNGC | 8 cut(s) | 810, 813, 828, 1130, 1133, 1434, 1610, 2613 |
| FspBI | CTAG | 4 cut(s) | 1835, 1913, 2333, 2616 |
| GlaI | GCGC | 2 cut(s) | 988, 1106 |
| GluI | GCNGC | 8 cut(s) | 810, 813, 828, 1130, 1133, 1434, 1610, 2613 |
| GsaI | CCCAGC | 1 cut(s) | 172 |
| GsuI | CTGGAG | 1 cut(s) | 1608 |
| HaeII | RGCGCY | 1 cut(s) | 1108 |
| HaeIII | GGCC | 1 cut(s) | 1644 |
| HapII | CCGG | 1 cut(s) | 2421 |
| HgaI | GACGC | 2 cut(s) | 1877, 2024 |
| HhaI | GCGC | 2 cut(s) | 989, 1107 |
| Hin1I | GRCGYC | 1 cut(s) | 2016 |
| Hin6I | GCGC | 2 cut(s) | 987, 1105 |
| HinP1I | GCGC | 2 cut(s) | 987, 1105 |
| HincII | GTYRAC | 1 cut(s) | 1867 |
| HindII | GTYRAC | 1 cut(s) | 1867 |
| HindIII | AAGCTT | 2 cut(s) | 1852, 2806 |
| HinfI | GANTC | 7 cut(s) | 560, 714, 739, 1330, 1472, 2245, 2708 |
| HpaII | CCGG | 1 cut(s) | 2421 |
| HphI | GGTGA | 7 cut(s) | 440, 487, 884, 1165, 1400, 2409, 2576 |
| Hpy188I | TCNGA | 9 cut(s) | 283, 358, 375, 406, 559, 579, 1424, 1477, 2481 |
| Hpy99I | CGWCG | 2 cut(s) | 2603, 2757 |
| HpyCH4III | ACNGT | 9 cut(s) | 221, 226, 1056, 1670, 2055, 2062, 2214, 2448, 2657 |
| HpyCH4IV | ACGT | 1 cut(s) | 2601 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 809, 818, 827, 947, 1289, 1533, 2015, 2040 |
| HpySE526I | ACGT | 1 cut(s) | 2601 |
| Hsp92I | GRCGYC | 1 cut(s) | 2016 |
| HspAI | GCGC | 2 cut(s) | 987, 1105 |
| LguI | GCTCTTC | 1 cut(s) | 255 |
| LmnI | GCTCC | 3 cut(s) | 506, 894, 2465 |
| Lsp1109I | GCAGC | 7 cut(s) | 796, 814, 824, 1116, 1119, 1621, 2624 |
| LweI | GCATC | 5 cut(s) | 814, 937, 1453, 1536, 1704 |
| MaeI | CTAG | 4 cut(s) | 1835, 1913, 2333, 2616 |
| MaeII | ACGT | 1 cut(s) | 2601 |
| MaeIII | GTNAC | 5 cut(s) | 421, 2002, 2203, 2401, 2822 |
| MfeI | CAATTG | 2 cut(s) | 182, 237 |
| MflI | RGATCY | 1 cut(s) | 33 |
| MhlI | GDGCHC | 7 cut(s) | 267, 511, 599, 899, 1812, 1834, 2226 |
| MlyI | GAGTC | 2 cut(s) | 723, 733 |
| MmeI | TCCRAC | 2 cut(s) | 1939, 2149 |
| Mph1103I | ATGCAT | 1 cut(s) | 1719 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2354 |
| MseI | TTAA | 6 cut(s) | 210, 677, 681, 1689, 1824, 2643 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1124, 2506 |
| MspA1I | CMGCKG | 1 cut(s) | 168 |
| MspI | CCGG | 1 cut(s) | 2421 |
| MspR9I | CCNGG | 5 cut(s) | 798, 1626, 1986, 2262, 2421 |
| MunI | CAATTG | 2 cut(s) | 182, 237 |
| Mva1269I | GAATGC | 1 cut(s) | 1492 |
| MvaI | CCWGG | 4 cut(s) | 798, 1626, 1986, 2262 |
| MvnI | CGCG | 2 cut(s) | 989, 2785 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 809, 818, 827, 947, 1289, 1533, 2015, 2040 |
| NciI | CCSGG | 1 cut(s) | 2421 |
| NcoI | CCATGG | 1 cut(s) | 1970 |
| NdeI | CATATG | 1 cut(s) | 700 |
| NlaIV | GGNNCC | 7 cut(s) | 35, 83, 467, 483, 1516, 2461, 2679 |
| NmuCI | GTSAC | 4 cut(s) | 421, 2002, 2203, 2401 |
| NsiI | ATGCAT | 1 cut(s) | 1719 |
| NspI | RCATGY | 1 cut(s) | 1283 |
| NspV | TTCGAA | 1 cut(s) | 1197 |
| OliI | CACNNNNGTG | 1 cut(s) | 1124 |
| PacI | TTAATTAA | 1 cut(s) | 681 |
| PagI | TCATGA | 1 cut(s) | 2843 |
| PaqCI | CACCTGC | 1 cut(s) | 860 |
| PciSI | GCTCTTC | 1 cut(s) | 255 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 2540 |
| PctI | GAATGC | 1 cut(s) | 1492 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2354 |
| PfeI | GAWTC | 5 cut(s) | 560, 1330, 1472, 2245, 2708 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 168, 1976 |
| PfoI | TCCNGGA | 1 cut(s) | 1624 |
| PkrI | GCNGC | 8 cut(s) | 811, 814, 829, 1131, 1134, 1435, 1611, 2614 |
| PleI | GAGTC | 2 cut(s) | 722, 733 |
| PpsI | GAGTC | 2 cut(s) | 722, 733 |
| PpuMI | RGGWCCY | 1 cut(s) | 1964 |
| Psp124BI | GAGCTC | 3 cut(s) | 511, 599, 899 |
| Psp5II | RGGWCCY | 1 cut(s) | 1964 |
| Psp6I | CCWGG | 4 cut(s) | 796, 1624, 1984, 2260 |
| PspFI | CCCAGC | 1 cut(s) | 168 |
| PspGI | CCWGG | 4 cut(s) | 796, 1624, 1984, 2260 |
| PspN4I | GGNNCC | 7 cut(s) | 35, 83, 467, 483, 1516, 2461, 2679 |
| PspPI | GGNCC | 6 cut(s) | 466, 1400, 1514, 1643, 1964, 2423 |
| PspPPI | RGGWCCY | 1 cut(s) | 1964 |
| PstI | CTGCAG | 2 cut(s) | 814, 871 |
| PstNI | CAGNNNCTG | 3 cut(s) | 521, 1108, 2140 |
| PsuI | RGATCY | 1 cut(s) | 33 |
| PvuII | CAGCTG | 1 cut(s) | 168 |
| RsaI | GTAC | 4 cut(s) | 223, 976, 1058, 2596 |
| RsaNI | GTAC | 4 cut(s) | 222, 975, 1057, 2595 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1124, 2506 |
| SacI | GAGCTC | 3 cut(s) | 511, 599, 899 |
| SapI | GCTCTTC | 1 cut(s) | 255 |
| SaqAI | TTAA | 6 cut(s) | 210, 677, 681, 1689, 1824, 2643 |
| SatI | GCNGC | 8 cut(s) | 810, 813, 828, 1130, 1133, 1434, 1610, 2613 |
| Sau96I | GGNCC | 6 cut(s) | 466, 1400, 1514, 1643, 1964, 2423 |
| ScaI | AGTACT | 1 cut(s) | 976 |
| SchI | GAGTC | 2 cut(s) | 723, 733 |
| ScrFI | CCNGG | 5 cut(s) | 798, 1626, 1986, 2262, 2421 |
| SduI | GDGCHC | 7 cut(s) | 267, 511, 599, 899, 1812, 1834, 2226 |
| SfaNI | GCATC | 5 cut(s) | 814, 937, 1453, 1536, 1704 |
| SfcI | CTRYAG | 5 cut(s) | 225, 810, 867, 2051, 2124 |
| SfuI | TTCGAA | 1 cut(s) | 1197 |
| SinI | GGWCC | 5 cut(s) | 466, 1400, 1514, 1964, 2423 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1124, 2506 |
| SmlI | CTYRAG | 2 cut(s) | 1230, 1936 |
| SmoI | CTYRAG | 2 cut(s) | 1230, 1936 |
| SpeI | ACTAGT | 1 cut(s) | 2332 |
| SsiI | CCGC | 3 cut(s) | 1433, 2390, 2520 |
| SspI | AATATT | 2 cut(s) | 1445, 1458 |
| SspMI | CTAG | 4 cut(s) | 1835, 1913, 2333, 2616 |
| SstI | GAGCTC | 3 cut(s) | 511, 599, 899 |
| StyD4I | CCNGG | 5 cut(s) | 796, 1624, 1984, 2260, 2419 |
| StyI | CCWWGG | 3 cut(s) | 1970, 2697, 2835 |
| TaaI | ACNGT | 9 cut(s) | 221, 226, 1056, 1670, 2055, 2062, 2214, 2448, 2657 |
| TaiI | ACGT | 1 cut(s) | 2604 |
| TaqI | TCGA | 4 cut(s) | 1197, 2358, 2534, 2557 |
| TatI | WGTACW | 2 cut(s) | 221, 974 |
| TauI | GCSGC | 1 cut(s) | 1436 |
| TfiI | GAWTC | 5 cut(s) | 560, 1330, 1472, 2245, 2708 |
| Tru1I | TTAA | 6 cut(s) | 210, 677, 681, 1689, 1824, 2643 |
| Tru9I | TTAA | 6 cut(s) | 210, 677, 681, 1689, 1824, 2643 |
| TscAI | CASTG | 9 cut(s) | 430, 476, 595, 1131, 1189, 1347, 1885, 1936, 2662 |
| TseFI | GTSAC | 4 cut(s) | 421, 2002, 2203, 2401 |
| TseI | GCWGC | 7 cut(s) | 809, 812, 827, 1129, 1132, 1609, 2612 |
| Tsp45I | GTSAC | 4 cut(s) | 421, 2002, 2203, 2401 |
| TspGWI | ACGGA | 2 cut(s) | 940, 1164 |
| TspRI | CASTG | 9 cut(s) | 430, 476, 595, 1131, 1189, 1347, 1885, 1936, 2662 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 168, 1976 |
| VneI | GTGCAC | 1 cut(s) | 1830 |
| VpaK11BI | GGWCC | 5 cut(s) | 466, 1400, 1514, 1964, 2423 |
| XapI | RAATTY | 4 cut(s) | 384, 1314, 1696, 1980 |
| XceI | RCATGY | 1 cut(s) | 1283 |
| XmiI | GTMKAC | 2 cut(s) | 615, 2604 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2354 |
| XspI | CTAG | 4 cut(s) | 1835, 1913, 2333, 2616 |
| ZrmI | AGTACT | 1 cut(s) | 976 |
| Zsp2I | ATGCAT | 1 cut(s) | 1719 |