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| Species | Orthologous Gene IDs |
|---|---|
| fragaria_vesca | FvH4_3g28450 FvH4_3g28450 |
| malus_domestica | MD03G1172000.v1.1 |
| prunus_persica | Prupe.4G254900_v2.0.a1 Prupe.4G254900_v2.0.a1 |
| pyrus_communis | pycom03g12910 |
| rosa_chinensis | RchiOBHm_Chr5g0053011 |
| rosa_laevigata | RLG00000034778 |
| rosa_multiflora | Rmu_sc0006573.1_g000003 |
| rosa_roxburghii | Rroxscaffold_1G00028830 |
| rosa_rugosa | Rorug05G0268800 |
| rosa_samantha | Rh5AG341100 Rh5BG351600 Rh5CG378000 Rh5DG368000 |
| rosa_wichuraiana | Rw5G032150 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 1904, 2051 |
| AarI | CACCTGC | 1 cut(s) | 1025 |
| AbsI | CCTCGAGG | 1 cut(s) | 2098 |
| Acc36I | ACCTGC | 1 cut(s) | 1025 |
| AccB1I | GGYRCC | 3 cut(s) | 53, 261, 2839 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 351, 668 |
| AccI | GTMKAC | 2 cut(s) | 295, 2344 |
| AciI | CCGC | 1 cut(s) | 2682 |
| AclWI | GGATC | 6 cut(s) | 593, 832, 1272, 1357, 2090, 2661 |
| AcoI | YGGCCR | 1 cut(s) | 1804 |
| AcsI | RAATTY | 6 cut(s) | 732, 1319, 1476, 1491, 1858, 2041 |
| AcuI | CTGAAG | 4 cut(s) | 744, 1659, 2198, 2733 |
| AfaI | GTAC | 7 cut(s) | 406, 1141, 1440, 1473, 1658, 1801, 2092 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 351, 469, 668, 1133, 2134, 2695, 2696 |
| AflIII | ACRYGT | 1 cut(s) | 2632 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1060 |
| AhlI | ACTAGT | 1 cut(s) | 2494 |
| AjnI | CCWGG | 6 cut(s) | 961, 1786, 2146, 2295, 2422, 2581 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 456, 488 |
| Alw21I | GWGCWC | 3 cut(s) | 676, 764, 1974 |
| Alw26I | GTCTC | 4 cut(s) | 1155, 1207, 1628, 2400 |
| AlwI | GGATC | 6 cut(s) | 593, 832, 1272, 1357, 2090, 2661 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 1637, 2302, 2823 |
| Ama87I | CYCGRG | 1 cut(s) | 2098 |
| AoxI | GGCC | 4 cut(s) | 1790, 1804, 2125, 2926 |
| ApeKI | GCWGC | 5 cut(s) | 977, 992, 1294, 1771, 2774 |
| ApoI | RAATTY | 6 cut(s) | 732, 1319, 1476, 1491, 1858, 2041 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1744, 1776 |
| AspLEI | GCGC | 1 cut(s) | 15 |
| AspS9I | GGNCC | 9 cut(s) | 4, 208, 425, 1562, 1676, 1790, 2126, 2585, 2927 |
| AsuC2I | CCSGG | 1 cut(s) | 1135 |
| AsuHPI | GGTGA | 4 cut(s) | 1049, 1432, 1562, 2571 |
| AsuII | TTCGAA | 1 cut(s) | 1359 |
| AsuNHI | GCTAGC | 1 cut(s) | 2074 |
| AvaI | CYCGRG | 1 cut(s) | 2098 |
| AvaII | GGWCC | 6 cut(s) | 4, 208, 425, 1562, 1676, 2585 |
| AxyI | CCTNAGG | 1 cut(s) | 2231 |
| BanI | GGYRCC | 3 cut(s) | 53, 261, 2839 |
| BanII | GRGCYC | 2 cut(s) | 676, 764 |
| BbsI | GAAGAC | 1 cut(s) | 682 |
| Bbv12I | GWGCWC | 3 cut(s) | 676, 764, 1974 |
| BbvCI | CCTCAGC | 1 cut(s) | 1961 |
| BbvI | GCAGC | 5 cut(s) | 979, 989, 1281, 1783, 2786 |
| BceAI | ACGGC | 1 cut(s) | 1791 |
| BciT130I | CCWGG | 6 cut(s) | 963, 1788, 2148, 2297, 2424, 2583 |
| BcnI | CCSGG | 1 cut(s) | 1135 |
| BcoDI | GTCTC | 4 cut(s) | 1155, 1207, 1628, 2400 |
| BcuI | ACTAGT | 1 cut(s) | 2494 |
| BfaI | CTAG | 6 cut(s) | 996, 1622, 2075, 2495, 2594, 2778 |
| BfmI | CTRYAG | 6 cut(s) | 408, 453, 632, 870, 1032, 2286 |
| BfuAI | ACCTGC | 1 cut(s) | 1025 |
| BglII | AGATCT | 3 cut(s) | 1889, 2153, 2767 |
| BisI | GCNGC | 5 cut(s) | 978, 993, 1295, 1772, 2775 |
| BlsI | GCNGC | 5 cut(s) | 979, 994, 1296, 1773, 2776 |
| BmcAI | AGTACT | 2 cut(s) | 1658, 1801 |
| Bme1390I | CCNGG | 7 cut(s) | 963, 1135, 1788, 2148, 2297, 2424, 2583 |
| Bme18I | GGWCC | 6 cut(s) | 4, 208, 425, 1562, 1676, 2585 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1060 |
| BmeT110I | CYCGRG | 1 cut(s) | 2098 |
| BmgT120I | GGNCC | 9 cut(s) | 4, 208, 425, 1562, 1676, 1790, 2126, 2585, 2927 |
| BmiI | GGNNCC | 7 cut(s) | 55, 209, 263, 474, 1677, 1678, 2841 |
| BmrFI | CCNGG | 7 cut(s) | 963, 1135, 1788, 2148, 2297, 2424, 2583 |
| BmrI | ACTGGG | 2 cut(s) | 285, 2542 |
| BmtI | GCTAGC | 1 cut(s) | 2078 |
| BmuI | ACTGGG | 2 cut(s) | 285, 2542 |
| BpiI | GAAGAC | 1 cut(s) | 682 |
| BpmI | CTGGAG | 2 cut(s) | 690, 984 |
| Bpu10I | CCTNAGC | 4 cut(s) | 1543, 1961, 2813, 2865 |
| Bpu14I | TTCGAA | 1 cut(s) | 1359 |
| BpuEI | CTTGAG | 2 cut(s) | 1092, 1377 |
| BpuMI | CCSGG | 1 cut(s) | 1135 |
| BsaI | GGTCTC | 1 cut(s) | 1155 |
| BsaJI | CCNNGG | 6 cut(s) | 49, 949, 961, 1787, 2132, 2997 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 957, 987, 2326, 2356 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 351, 469, 668, 1133, 2134, 2695, 2696 |
| Bse21I | CCTNAGG | 1 cut(s) | 2231 |
| Bse3DI | GCAATG | 4 cut(s) | 2148, 2439, 2444, 2523 |
| BseBI | CCWGG | 6 cut(s) | 963, 1788, 2148, 2297, 2424, 2583 |
| BseDI | CCNNGG | 6 cut(s) | 49, 949, 961, 1787, 2132, 2997 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 351, 469, 668, 1133, 2134, 2695, 2696 |
| BseMI | GCAATG | 4 cut(s) | 2148, 2439, 2444, 2523 |
| BseXI | GCAGC | 5 cut(s) | 979, 989, 1281, 1783, 2786 |
| BseYI | CCCAGC | 1 cut(s) | 351 |
| BsgI | GTGCAG | 4 cut(s) | 874, 1645, 2013, 2740 |
| BshFI | GGCC | 4 cut(s) | 1792, 1806, 2127, 2928 |
| BshNI | GGYRCC | 3 cut(s) | 53, 261, 2839 |
| BsiHKAI | GWGCWC | 3 cut(s) | 676, 764, 1974 |
| BsiHKCI | CYCGRG | 1 cut(s) | 2098 |
| BsiSI | CCGG | 1 cut(s) | 1134 |
| BslFI | GGGAC | 2 cut(s) | 916, 1689 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 351, 469, 668, 1133, 2134, 2695, 2696 |
| BsmAI | GTCTC | 4 cut(s) | 1155, 1207, 1628, 2400 |
| BsmBI | CGTCTC | 1 cut(s) | 1207 |
| BsmFI | GGGAC | 2 cut(s) | 916, 1689 |
| BsmI | GAATGC | 2 cut(s) | 1654, 2518 |
| BsnI | GGCC | 4 cut(s) | 1792, 1806, 2127, 2928 |
| Bso31I | GGTCTC | 1 cut(s) | 1155 |
| BsoBI | CYCGRG | 1 cut(s) | 2098 |
| Bsp119I | TTCGAA | 1 cut(s) | 1359 |
| Bsp1286I | GDGCHC | 3 cut(s) | 676, 764, 1974 |
| Bsp1407I | TGTACA | 1 cut(s) | 1471 |
| Bsp19I | CCATGG | 2 cut(s) | 949, 2132 |
| BspACI | CCGC | 1 cut(s) | 2682 |
| BspANI | GGCC | 4 cut(s) | 1792, 1806, 2127, 2928 |
| BspHI | TCATGA | 1 cut(s) | 3005 |
| BspLI | GGNNCC | 7 cut(s) | 55, 209, 263, 474, 1677, 1678, 2841 |
| BspMAI | CTGCAG | 2 cut(s) | 874, 1036 |
| BspMI | ACCTGC | 1 cut(s) | 1025 |
| BspOI | GCTAGC | 1 cut(s) | 2078 |
| BspPI | GGATC | 6 cut(s) | 593, 832, 1272, 1357, 2090, 2661 |
| BspT104I | TTCGAA | 1 cut(s) | 1359 |
| BspT107I | GGYRCC | 3 cut(s) | 53, 261, 2839 |
| BspTNI | GGTCTC | 1 cut(s) | 1155 |
| BsrDI | GCAATG | 4 cut(s) | 2148, 2439, 2444, 2523 |
| BsrGI | TGTACA | 1 cut(s) | 1471 |
| BssECI | CCNNGG | 6 cut(s) | 49, 949, 961, 1787, 2132, 2997 |
| BssT1I | CCWWGG | 4 cut(s) | 49, 949, 2132, 2997 |
| Bst2UI | CCWGG | 6 cut(s) | 963, 1788, 2148, 2297, 2424, 2583 |
| Bst6I | CTCTTC | 1 cut(s) | 2189 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 974, 1520 |
| BstAUI | TGTACA | 1 cut(s) | 1471 |
| BstBI | TTCGAA | 1 cut(s) | 1359 |
| BstC8I | GCNNGC | 2 cut(s) | 2072, 2076 |
| BstDSI | CCRYGG | 2 cut(s) | 949, 2132 |
| BstHHI | GCGC | 1 cut(s) | 15 |
| BstMAI | GTCTC | 4 cut(s) | 1155, 1207, 1628, 2400 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 974, 983, 992, 1112, 1451, 1520, 1771, 2202 |
| BstNI | CCWGG | 6 cut(s) | 963, 1788, 2148, 2297, 2424, 2583 |
| BstNSI | RCATGY | 2 cut(s) | 1445, 2636 |
| BstSCI | CCNGG | 7 cut(s) | 961, 1133, 1786, 2146, 2295, 2422, 2581 |
| BstSFI | CTRYAG | 6 cut(s) | 408, 453, 632, 870, 1032, 2286 |
| BstV1I | GCAGC | 5 cut(s) | 979, 989, 1281, 1783, 2786 |
| BstV2I | GAAGAC | 1 cut(s) | 682 |
| BstX2I | RGATCY | 4 cut(s) | 1349, 1889, 2153, 2767 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1416, 1845 |
| BstYI | RGATCY | 4 cut(s) | 1349, 1889, 2153, 2767 |
| Bsu36I | CCTNAGG | 1 cut(s) | 2231 |
| BsuRI | GGCC | 4 cut(s) | 1792, 1806, 2127, 2928 |
| BtgI | CCRYGG | 2 cut(s) | 949, 2132 |
| BtgZI | GCGATG | 1 cut(s) | 1889 |
| BtsI | GCAGTG | 1 cut(s) | 2824 |
| BveI | ACCTGC | 1 cut(s) | 1025 |
| Cac8I | GCNNGC | 2 cut(s) | 2072, 2076 |
| CaiI | CAGNNNCTG | 3 cut(s) | 1637, 2302, 2823 |
| CciI | TCATGA | 1 cut(s) | 3005 |
| CfoI | GCGC | 1 cut(s) | 15 |
| Cfr13I | GGNCC | 9 cut(s) | 4, 208, 425, 1562, 1676, 1790, 2126, 2585, 2927 |
| CseI | GACGC | 2 cut(s) | 243, 1313 |
| CsiI | ACCWGGT | 1 cut(s) | 2581 |
| Csp6I | GTAC | 7 cut(s) | 405, 1140, 1439, 1472, 1657, 1800, 2091 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1214, 1249 |
| CviQI | GTAC | 7 cut(s) | 405, 1140, 1439, 1472, 1657, 1800, 2091 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1060 |
| EaeI | YGGCCR | 1 cut(s) | 1804 |
| Eam1104I | CTCTTC | 1 cut(s) | 2189 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1060 |
| EarI | CTCTTC | 1 cut(s) | 2189 |
| Ecl136II | GAGCTC | 2 cut(s) | 674, 762 |
| Eco130I | CCWWGG | 4 cut(s) | 49, 949, 2132, 2997 |
| Eco24I | GRGCYC | 2 cut(s) | 676, 764 |
| Eco31I | GGTCTC | 1 cut(s) | 1155 |
| Eco47I | GGWCC | 6 cut(s) | 4, 208, 425, 1562, 1676, 2585 |
| Eco53kI | GAGCTC | 2 cut(s) | 674, 762 |
| Eco57I | CTGAAG | 4 cut(s) | 744, 1659, 2198, 2733 |
| Eco81I | CCTNAGG | 1 cut(s) | 2231 |
| Eco88I | CYCGRG | 1 cut(s) | 2098 |
| EcoICRI | GAGCTC | 2 cut(s) | 674, 762 |
| EcoO109I | RGGNCCY | 3 cut(s) | 208, 1676, 2126 |
| EcoRI | GAATTC | 2 cut(s) | 732, 1491 |
| EcoRII | CCWGG | 6 cut(s) | 961, 1786, 2146, 2295, 2422, 2581 |
| EcoT14I | CCWWGG | 4 cut(s) | 49, 949, 2132, 2997 |
| EcoT22I | ATGCAT | 3 cut(s) | 577, 1434, 1881 |
| EcoT38I | GRGCYC | 2 cut(s) | 676, 764 |
| ErhI | CCWWGG | 4 cut(s) | 49, 949, 2132, 2997 |
| Esp3I | CGTCTC | 1 cut(s) | 1207 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 65, 97 |
| FaqI | GGGAC | 2 cut(s) | 916, 1689 |
| FauNDI | CATATG | 3 cut(s) | 9, 1309, 1428 |
| FblI | GTMKAC | 2 cut(s) | 295, 2344 |
| Fnu4HI | GCNGC | 5 cut(s) | 978, 993, 1295, 1772, 2775 |
| FriOI | GRGCYC | 2 cut(s) | 676, 764 |
| Fsp4HI | GCNGC | 5 cut(s) | 978, 993, 1295, 1772, 2775 |
| FspBI | CTAG | 6 cut(s) | 996, 1622, 2075, 2495, 2594, 2778 |
| GlaI | GCGC | 1 cut(s) | 14 |
| GluI | GCNGC | 5 cut(s) | 978, 993, 1295, 1772, 2775 |
| GsaI | CCCAGC | 1 cut(s) | 355 |
| GsuI | CTGGAG | 2 cut(s) | 690, 984 |
| HaeIII | GGCC | 4 cut(s) | 1792, 1806, 2127, 2928 |
| HapII | CCGG | 1 cut(s) | 1134 |
| HgaI | GACGC | 2 cut(s) | 243, 1313 |
| HhaI | GCGC | 1 cut(s) | 15 |
| Hin6I | GCGC | 1 cut(s) | 13 |
| HinP1I | GCGC | 1 cut(s) | 13 |
| HincII | GTYRAC | 3 cut(s) | 2029, 2293, 2345 |
| HindII | GTYRAC | 3 cut(s) | 2029, 2293, 2345 |
| HindIII | AAGCTT | 1 cut(s) | 2014 |
| HinfI | GANTC | 8 cut(s) | 27, 86, 904, 1386, 1405, 2792, 2870, 2949 |
| HpaII | CCGG | 1 cut(s) | 1134 |
| HphI | GGTGA | 4 cut(s) | 1049, 1432, 1562, 2571 |
| HpyAV | CCTTC | 8 cut(s) | 133, 812, 1634, 2103, 2591, 2726, 2782, 2918 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 974, 983, 992, 1112, 1451, 1520, 1771, 2202 |
| HspAI | GCGC | 1 cut(s) | 13 |
| KflI | GGGWCCC | 1 cut(s) | 1676 |
| LmnI | GCTCC | 3 cut(s) | 337, 671, 965 |
| Lsp1109I | GCAGC | 5 cut(s) | 979, 989, 1281, 1783, 2786 |
| MabI | ACCWGGT | 1 cut(s) | 2581 |
| MaeI | CTAG | 6 cut(s) | 996, 1622, 2075, 2495, 2594, 2778 |
| MaeIII | GTNAC | 7 cut(s) | 1738, 2063, 2365, 2563, 2819, 2943, 2984 |
| MfeI | CAATTG | 2 cut(s) | 420, 2706 |
| MflI | RGATCY | 4 cut(s) | 1349, 1889, 2153, 2767 |
| MhlI | GDGCHC | 3 cut(s) | 676, 764, 1974 |
| MlyI | GAGTC | 2 cut(s) | 898, 1414 |
| MmeI | TCCRAC | 4 cut(s) | 452, 1625, 2101, 2987 |
| Mph1103I | ATGCAT | 3 cut(s) | 577, 1434, 1881 |
| MseI | TTAA | 6 cut(s) | 135, 393, 846, 1781, 1851, 1986 |
| MslI | CAYNNNNRTG | 2 cut(s) | 2511, 2668 |
| MspA1I | CMGCKG | 1 cut(s) | 351 |
| MspI | CCGG | 1 cut(s) | 1134 |
| MspR9I | CCNGG | 7 cut(s) | 963, 1135, 1788, 2148, 2297, 2424, 2583 |
| MunI | CAATTG | 2 cut(s) | 420, 2706 |
| Mva1269I | GAATGC | 2 cut(s) | 1654, 2518 |
| MvaI | CCWGG | 6 cut(s) | 963, 1788, 2148, 2297, 2424, 2583 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 974, 983, 992, 1112, 1451, 1520, 1771, 2202 |
| NciI | CCSGG | 1 cut(s) | 1135 |
| NcoI | CCATGG | 2 cut(s) | 949, 2132 |
| NdeI | CATATG | 3 cut(s) | 9, 1309, 1428 |
| NheI | GCTAGC | 1 cut(s) | 2074 |
| NlaIV | GGNNCC | 7 cut(s) | 55, 209, 263, 474, 1677, 1678, 2841 |
| NmuCI | GTSAC | 6 cut(s) | 1738, 2063, 2365, 2563, 2819, 2943 |
| NsiI | ATGCAT | 3 cut(s) | 577, 1434, 1881 |
| NspI | RCATGY | 2 cut(s) | 1445, 2636 |
| NspV | TTCGAA | 1 cut(s) | 1359 |
| PaeR7I | CTCGAG | 1 cut(s) | 2098 |
| PagI | TCATGA | 1 cut(s) | 3005 |
| PaqCI | CACCTGC | 1 cut(s) | 1025 |
| PciI | ACATGT | 1 cut(s) | 2632 |
| PctI | GAATGC | 2 cut(s) | 1654, 2518 |
| PfeI | GAWTC | 6 cut(s) | 27, 86, 1386, 2792, 2870, 2949 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 351, 668 |
| PkrI | GCNGC | 5 cut(s) | 979, 994, 1296, 1773, 2776 |
| PleI | GAGTC | 2 cut(s) | 898, 1413 |
| PpsI | GAGTC | 2 cut(s) | 898, 1413 |
| PpuMI | RGGWCCY | 2 cut(s) | 208, 1676 |
| PscI | ACATGT | 1 cut(s) | 2632 |
| PsiI | TTATAA | 2 cut(s) | 1904, 2051 |
| Psp124BI | GAGCTC | 2 cut(s) | 676, 764 |
| Psp5II | RGGWCCY | 2 cut(s) | 208, 1676 |
| Psp6I | CCWGG | 6 cut(s) | 961, 1786, 2146, 2295, 2422, 2581 |
| PspFI | CCCAGC | 1 cut(s) | 351 |
| PspGI | CCWGG | 6 cut(s) | 961, 1786, 2146, 2295, 2422, 2581 |
| PspN4I | GGNNCC | 7 cut(s) | 55, 209, 263, 474, 1677, 1678, 2841 |
| PspPI | GGNCC | 9 cut(s) | 4, 208, 425, 1562, 1676, 1790, 2126, 2585, 2927 |
| PspPPI | RGGWCCY | 2 cut(s) | 208, 1676 |
| PspXI | VCTCGAGB | 1 cut(s) | 2098 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 2539, 2571 |
| PstI | CTGCAG | 2 cut(s) | 874, 1036 |
| PstNI | CAGNNNCTG | 3 cut(s) | 1637, 2302, 2823 |
| PsuI | RGATCY | 4 cut(s) | 1349, 1889, 2153, 2767 |
| PvuII | CAGCTG | 1 cut(s) | 351 |
| RsaI | GTAC | 7 cut(s) | 406, 1141, 1440, 1473, 1658, 1801, 2092 |
| RsaNI | GTAC | 7 cut(s) | 405, 1140, 1439, 1472, 1657, 1800, 2091 |
| RseI | CAYNNNNRTG | 2 cut(s) | 2511, 2668 |
| SacI | GAGCTC | 2 cut(s) | 676, 764 |
| SalI | GTCGAC | 1 cut(s) | 2343 |
| SaqAI | TTAA | 6 cut(s) | 135, 393, 846, 1781, 1851, 1986 |
| SatI | GCNGC | 5 cut(s) | 978, 993, 1295, 1772, 2775 |
| Sau96I | GGNCC | 9 cut(s) | 4, 208, 425, 1562, 1676, 1790, 2126, 2585, 2927 |
| ScaI | AGTACT | 2 cut(s) | 1658, 1801 |
| SchI | GAGTC | 2 cut(s) | 898, 1414 |
| ScrFI | CCNGG | 7 cut(s) | 963, 1135, 1788, 2148, 2297, 2424, 2583 |
| SduI | GDGCHC | 3 cut(s) | 676, 764, 1974 |
| SexAI | ACCWGGT | 1 cut(s) | 2581 |
| SfcI | CTRYAG | 6 cut(s) | 408, 453, 632, 870, 1032, 2286 |
| Sfr274I | CTCGAG | 1 cut(s) | 2098 |
| SfuI | TTCGAA | 1 cut(s) | 1359 |
| SinI | GGWCC | 6 cut(s) | 4, 208, 425, 1562, 1676, 2585 |
| SlaI | CTCGAG | 1 cut(s) | 2098 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 2511, 2668 |
| SmlI | CTYRAG | 3 cut(s) | 1107, 1392, 2098 |
| SmoI | CTYRAG | 3 cut(s) | 1107, 1392, 2098 |
| SpeI | ACTAGT | 1 cut(s) | 2494 |
| SsiI | CCGC | 1 cut(s) | 2682 |
| SspMI | CTAG | 6 cut(s) | 996, 1622, 2075, 2495, 2594, 2778 |
| SstI | GAGCTC | 2 cut(s) | 676, 764 |
| StyD4I | CCNGG | 7 cut(s) | 961, 1133, 1786, 2146, 2295, 2422, 2581 |
| StyI | CCWWGG | 4 cut(s) | 49, 949, 2132, 2997 |
| TaqI | TCGA | 4 cut(s) | 1359, 1796, 2099, 2344 |
| TatI | WGTACW | 6 cut(s) | 404, 1139, 1471, 1656, 1799, 2090 |
| TfiI | GAWTC | 6 cut(s) | 27, 86, 1386, 2792, 2870, 2949 |
| Tru1I | TTAA | 6 cut(s) | 135, 393, 846, 1781, 1851, 1986 |
| Tru9I | TTAA | 6 cut(s) | 135, 393, 846, 1781, 1851, 1986 |
| TseFI | GTSAC | 6 cut(s) | 1738, 2063, 2365, 2563, 2819, 2943 |
| TseI | GCWGC | 5 cut(s) | 977, 992, 1294, 1771, 2774 |
| Tsp45I | GTSAC | 6 cut(s) | 1738, 2063, 2365, 2563, 2819, 2943 |
| TspGWI | ACGGA | 1 cut(s) | 2052 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 351, 668 |
| VpaK11BI | GGWCC | 6 cut(s) | 4, 208, 425, 1562, 1676, 2585 |
| XapI | RAATTY | 6 cut(s) | 732, 1319, 1476, 1491, 1858, 2041 |
| XceI | RCATGY | 2 cut(s) | 1445, 2636 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2937 |
| XhoI | CTCGAG | 1 cut(s) | 2098 |
| XmiI | GTMKAC | 2 cut(s) | 295, 2344 |
| XspI | CTAG | 6 cut(s) | 996, 1622, 2075, 2495, 2594, 2778 |
| ZrmI | AGTACT | 2 cut(s) | 1658, 1801 |
| Zsp2I | ATGCAT | 3 cut(s) | 577, 1434, 1881 |