FvH4_3g34900

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0018009
Physical Location & Seq
Reverse (-)
30142999 .. 30143604
606 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g34900.t1

Sequence Viewer

Length: 606 bp
ATGACATCCCCAGGTTCTCGTCCTTCGCAATTGGATCCGTGGCTCATTGCTTCTGTTCTTATCCTCTGCACTGTTGCTGTCATCTTCAGTTATTACAAGATACTGAAGCGGTTTTGGTATGCATTTCGCTATGGAAGATCAAATCCAAATCACAGACGTACCCTTCTGAACGAGACCAACGCAGATGATGCATCTCTACTGTATCAAAGCCAGGGACTAGATTTCGCTGTTATTCGGTCTCTTCCCATAACTCAGTTCAAGAAGAAAAGTGAAGCAGAAGATGCAGAAGACAACCAAAGCAACACAGACTGTCCAGTATGCTTGGGTGAATTTGAAGAAGAGGAATGGCTAAGACATCTGCCCAAATGCAATCATGCATTCCACATATCTTGCATTGATACATGGTTTGTGGCTCACTCAAATTGCCCCTTATGCAGATCAGAGGTCTATGATTTCCACCAGGATTGTCCTATTCCTATGTACACTCTATTAGAGAGTGTCAGAGAAAGCGTTATTCAAGAAAGAGCATTACACTACCAAATGCTGCGTTCTGCCATCTTGGAGAACCAAAGCAATGAAGCCAGATACGACAATCACAATGTGTAG

Protein Analysis

202

Amino Acids

23.4

Weight (kDa)

5.59

Isoelectric Point (pI)

65.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 99 - 146 7.5e-07 RING-H2 zinc finger domain
zf-RING_2 PF13639 103 - 146 6.2e-13 Ring finger domain
zf-RING_11 PF17123 103 - 132 3e-08 RING-like zinc finger
zf-C3HC4_2 PF13923 104 - 145 3.7e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 104 - 145 8.6e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0018009)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g34900
malus_domestica MD11G1114600.v1.1
prunus_persica Prupe.6G085400_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0062771
rosa_laevigata RLG00000035576
rosa_multiflora Rmu_ssc0000472.1_g000010
rosa_roxburghii Rroxscaffold_1G00017850
rosa_rugosa Rorug05G0351600
rosa_samantha Rh5AG412500 Rh5DG440300
rosa_wichuraiana Rw5G038770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 109
AclWI GGATC 2 cut(s) 29, 42
AcsI RAATTY 1 cut(s) 329
AcuI CTGAAG 2 cut(s) 70, 125
AdeI CACNNNGTG 1 cut(s) 601
AfaI GTAC 2 cut(s) 160, 482
AgsI TTSAA 3 cut(s) 259, 335, 518
AjnI CCWGG 3 cut(s) 10, 210, 459
Alw26I GTCTC 2 cut(s) 167, 243
AlwI GGATC 2 cut(s) 29, 42
ApeKI GCWGC 1 cut(s) 544
ApoI RAATTY 1 cut(s) 329
AsuHPI GGTGA 1 cut(s) 338
BaeI ACNNNNGTAYC 2 cut(s) 142, 175
BamHI GGATCC 1 cut(s) 34
BbsI GAAGAC 1 cut(s) 294
BbvI GCAGC 1 cut(s) 531
BccI CCATC 1 cut(s) 563
BciT130I CCWGG 3 cut(s) 12, 212, 461
BcoDI GTCTC 2 cut(s) 167, 243
BfaI CTAG 1 cut(s) 218
BisI GCNGC 1 cut(s) 545
BlsI GCNGC 1 cut(s) 546
Bme1390I CCNGG 3 cut(s) 12, 212, 461
BmiI GGNNCC 1 cut(s) 36
BmrFI CCNGG 3 cut(s) 12, 212, 461
BmsI GCATC 3 cut(s) 178, 200, 271
BpiI GAAGAC 1 cut(s) 294
BsaI GGTCTC 2 cut(s) 167, 243
BsaJI CCNNGG 3 cut(s) 10, 38, 211
Bse1I ACTGG 1 cut(s) 314
Bse3DI GCAATG 2 cut(s) 45, 580
BseBI CCWGG 3 cut(s) 12, 212, 461
BseDI CCNNGG 3 cut(s) 10, 38, 211
BseGI GGATG 1 cut(s) 5
BseMI GCAATG 2 cut(s) 45, 580
BseMII CTCAG 1 cut(s) 266
BseNI ACTGG 1 cut(s) 314
BseXI GCAGC 1 cut(s) 531
BsgI GTGCAG 1 cut(s) 52
BslFI GGGAC 1 cut(s) 228
BsmAI GTCTC 2 cut(s) 167, 243
BsmFI GGGAC 1 cut(s) 228
BsmI GAATGC 1 cut(s) 377
Bso31I GGTCTC 2 cut(s) 167, 243
Bsp1407I TGTACA 1 cut(s) 480
Bsp143I GATC 3 cut(s) 34, 137, 437
BspACI CCGC 1 cut(s) 109
BspCNI CTCAG 1 cut(s) 265
BspLI GGNNCC 1 cut(s) 36
BspPI GGATC 2 cut(s) 29, 42
BspTNI GGTCTC 2 cut(s) 167, 243
BsrDI GCAATG 2 cut(s) 45, 580
BsrGI TGTACA 1 cut(s) 480
BsrI ACTGG 1 cut(s) 314
BssECI CCNNGG 3 cut(s) 10, 38, 211
BssMI GATC 3 cut(s) 34, 137, 437
Bst2UI CCWGG 3 cut(s) 12, 212, 461
Bst4CI ACNGT 3 cut(s) 73, 201, 311
Bst6I CTCTTC 2 cut(s) 246, 333
BstAPI GCANNNNNTGC 2 cut(s) 188, 281
BstAUI TGTACA 1 cut(s) 480
BstDEI CTNAG 2 cut(s) 252, 350
BstDSI CCRYGG 1 cut(s) 38
BstF5I GGATG 1 cut(s) 5
BstKTI GATC 3 cut(s) 37, 140, 440
BstMAI GTCTC 2 cut(s) 167, 243
BstMBI GATC 3 cut(s) 34, 137, 437
BstMWI GCNNNNNNNGC 3 cut(s) 188, 281, 432
BstNI CCWGG 3 cut(s) 12, 212, 461
BstSCI CCNGG 3 cut(s) 10, 210, 459
BstV1I GCAGC 1 cut(s) 531
BstV2I GAAGAC 1 cut(s) 294
BstX2I RGATCY 1 cut(s) 34
BstYI RGATCY 1 cut(s) 34
BtgI CCRYGG 1 cut(s) 38
BtsCI GGATG 1 cut(s) 5
BtsIMutI CAGTG 1 cut(s) 69
Csp6I GTAC 2 cut(s) 159, 481
CspCI CAANNNNNGTGG 4 cut(s) 371, 406, 446, 481
CviAII CATG 2 cut(s) 374, 402
CviJI RGCY 5 cut(s) 43, 210, 349, 413, 581
CviKI_1 RGCY 5 cut(s) 43, 210, 349, 413, 581
CviQI GTAC 2 cut(s) 159, 481
DdeI CTNAG 2 cut(s) 252, 350
DpnI GATC 3 cut(s) 36, 139, 439
DpnII GATC 3 cut(s) 34, 137, 437
DraIII CACNNNGTG 1 cut(s) 601
Eam1104I CTCTTC 2 cut(s) 246, 333
EarI CTCTTC 2 cut(s) 246, 333
Eco31I GGTCTC 2 cut(s) 167, 243
Eco57I CTGAAG 2 cut(s) 70, 125
EcoRII CCWGG 3 cut(s) 10, 210, 459
EcoT22I ATGCAT 3 cut(s) 124, 193, 379
FaeI CATG 2 cut(s) 377, 405
FaqI GGGAC 1 cut(s) 228
FatI CATG 2 cut(s) 373, 401
Fnu4HI GCNGC 1 cut(s) 545
Fsp4HI GCNGC 1 cut(s) 545
FspBI CTAG 1 cut(s) 218
GluI GCNGC 1 cut(s) 545
Hin1II CATG 2 cut(s) 377, 405
HphI GGTGA 1 cut(s) 338
Hpy166II GTNNAC 1 cut(s) 483
Hpy188I TCNGA 3 cut(s) 168, 442, 503
Hpy188III TCNNGA 2 cut(s) 259, 518
Hpy8I GTNNAC 1 cut(s) 483
HpyAV CCTTC 2 cut(s) 33, 173
HpyCH4III ACNGT 3 cut(s) 73, 201, 311
HpyCH4IV ACGT 1 cut(s) 157
HpyCH4V TGCA 8 cut(s) 69, 122, 191, 284, 369, 377, 393, 435
HpyF10VI GCNNNNNNNGC 3 cut(s) 188, 281, 432
HpyF3I CTNAG 2 cut(s) 252, 350
HpySE526I ACGT 1 cut(s) 157
Hsp92II CATG 2 cut(s) 377, 405
Kzo9I GATC 3 cut(s) 34, 137, 437
LpnPI CCDG 7 cut(s) 24, 197, 224, 327, 446, 473, 595
Lsp1109I GCAGC 1 cut(s) 531
LweI GCATC 3 cut(s) 178, 200, 271
MaeI CTAG 1 cut(s) 218
MaeII ACGT 1 cut(s) 157
MalI GATC 3 cut(s) 36, 139, 439
MboI GATC 3 cut(s) 34, 137, 437
MboII GAAGA 8 cut(s) 76, 147, 233, 274, 290, 299, 347, 350
MfeI CAATTG 1 cut(s) 29
MflI RGATCY 1 cut(s) 34
MluCI AATT 3 cut(s) 29, 329, 421
MnlI CCTC 3 cut(s) 74, 334, 436
Mph1103I ATGCAT 3 cut(s) 124, 193, 379
MspR9I CCNGG 3 cut(s) 12, 212, 461
MunI CAATTG 1 cut(s) 29
Mva1269I GAATGC 1 cut(s) 377
MvaI CCWGG 3 cut(s) 12, 212, 461
MwoI GCNNNNNNNGC 3 cut(s) 188, 281, 432
NdeII GATC 3 cut(s) 34, 137, 437
NlaIII CATG 2 cut(s) 377, 405
NlaIV GGNNCC 1 cut(s) 36
NsiI ATGCAT 3 cut(s) 124, 193, 379
PctI GAATGC 1 cut(s) 377
PkrI GCNGC 1 cut(s) 546
Psp6I CCWGG 3 cut(s) 10, 210, 459
PspGI CCWGG 3 cut(s) 10, 210, 459
PspN4I GGNNCC 1 cut(s) 36
PsuI RGATCY 1 cut(s) 34
RsaI GTAC 2 cut(s) 160, 482
RsaNI GTAC 2 cut(s) 159, 481
SatI GCNGC 1 cut(s) 545
Sau3AI GATC 3 cut(s) 34, 137, 437
ScrFI CCNGG 3 cut(s) 12, 212, 461
SetI ASST 3 cut(s) 16, 160, 447
SfaNI GCATC 3 cut(s) 178, 200, 271
Sse9I AATT 3 cut(s) 29, 329, 421
SsiI CCGC 1 cut(s) 109
SspMI CTAG 1 cut(s) 218
StyD4I CCNGG 3 cut(s) 10, 210, 459
TaaI ACNGT 3 cut(s) 73, 201, 311
TaiI ACGT 1 cut(s) 160
TaqII GACCGA 1 cut(s) 225
TasI AATT 3 cut(s) 29, 329, 421
TatI WGTACW 1 cut(s) 480
TscAI CASTG 1 cut(s) 76
TseI GCWGC 1 cut(s) 544
TspDTI ATGAA 1 cut(s) 591
TspGWI ACGGA 1 cut(s) 27
TspRI CASTG 1 cut(s) 76
XapI RAATTY 1 cut(s) 329
XspI CTAG 1 cut(s) 218
Zsp2I ATGCAT 3 cut(s) 124, 193, 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.