Rh5AG412500

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0018009
Physical Location & Seq
Reverse (-)
71243529 .. 71244596
1068 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG412500.1

Sequence Viewer

Length: 615 bp
ATGGTCATCCCTTCAACCCCTCCAATGACATCCCCAGGTTCTCGTCATCCGGATTCGTGGCTCATTGCTTCTGTTGCTATCCTCTGCACTTTTACTGTCATCTTCAGTTATTGCAAGATACTGAAACGGTGCAATACAAGATCCAATCCAAACCACAGACGTACCCTTCAGAACGAGACCAACGCAGATGATCCATCTCTGCAGTTTCAAAGCCAAGGACTAGATTTCGCAATCATCCGGTCTCTACCCATAACTCAGTTTAAGAAGAGAAGTGTAGCAGAAGATGCAGAAGAGCACCAGAGCAACACAGACTGTCCAGTATGCTTGGGTGAATTTGAAGAAGGGGAATGGCTAAGACATCTACCCAAATGCAATCATGCATTCCACATATCTTGCATCGACACTTGGTTTGTGGCTCACTCAAATTGCCCCTTATGCAGATCAGAAGTCTATGATTTTATCCAGGATTGTCCTATTCCTATGCACACTCTGCTAGAGACACTGAGGGAAAGTGTCATTGAAGAAAGAGCGTTACACTTCCACATGCTGCGCTCTGCGGTCTTGGAGAACTCACCATTTGGACGTGAAGCAAGATTCAGCGACGGCAACGTGTAG

Protein Analysis

204

Amino Acids

23.29

Weight (kDa)

5.9

Isoelectric Point (pI)

67.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 104 - 147 4.1e-13 Ring finger domain
zf-RING_11 PF17123 104 - 133 7e-09 RING-like zinc finger
zf-rbx1 PF12678 104 - 147 4.1e-06 RING-H2 zinc finger domain
zf-C3HC4_2 PF13923 105 - 146 7.1e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 105 - 146 1.6e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0018009)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g34900
malus_domestica MD11G1114600.v1.1
prunus_persica Prupe.6G085400_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0062771
rosa_laevigata RLG00000035576
rosa_multiflora Rmu_ssc0000472.1_g000010
rosa_roxburghii Rroxscaffold_1G00017850
rosa_rugosa Rorug05G0351600
rosa_samantha Rh5AG412500 Rh5DG440300
rosa_wichuraiana Rw5G038770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 49
AciI CCGC 1 cut(s) 557
AclWI GGATC 2 cut(s) 135, 185
AcsI RAATTY 1 cut(s) 332
AcuI CTGAAG 2 cut(s) 88, 152
AfaI GTAC 1 cut(s) 163
AflIII ACRYGT 1 cut(s) 609
AgsI TTSAA 4 cut(s) 15, 209, 338, 521
AjiI CACGTC 1 cut(s) 584
AjnI CCWGG 2 cut(s) 34, 462
Alw21I GWGCWC 1 cut(s) 297
Alw26I GTCTC 3 cut(s) 170, 246, 491
AlwI GGATC 2 cut(s) 135, 185
Aor13HI TCCGGA 1 cut(s) 49
ApeKI GCWGC 1 cut(s) 547
ApoI RAATTY 1 cut(s) 332
ArsI GACNNNNNNTTYG 2 cut(s) 392, 424
AspLEI GCGC 1 cut(s) 552
AsuHPI GGTGA 2 cut(s) 341, 564
BaeI ACNNNNGTAYC 2 cut(s) 145, 178
Bbv12I GWGCWC 1 cut(s) 297
BbvI GCAGC 1 cut(s) 534
BccI CCATC 1 cut(s) 202
BciT130I CCWGG 2 cut(s) 36, 464
BcoDI GTCTC 3 cut(s) 170, 246, 491
BfaI CTAG 2 cut(s) 221, 494
BfmI CTRYAG 1 cut(s) 200
BisI GCNGC 1 cut(s) 548
BlsI GCNGC 1 cut(s) 549
Bme1390I CCNGG 2 cut(s) 36, 464
BmgBI CACGTC 1 cut(s) 584
BmrFI CCNGG 2 cut(s) 36, 464
BmsI GCATC 2 cut(s) 274, 405
BsaI GGTCTC 2 cut(s) 170, 246
BsaJI CCNNGG 2 cut(s) 34, 214
BsaWI WCCGGW 2 cut(s) 49, 237
Bse1I ACTGG 1 cut(s) 317
Bse3DI GCAATG 1 cut(s) 63
BseAI TCCGGA 1 cut(s) 49
BseBI CCWGG 2 cut(s) 36, 464
BseDI CCNNGG 2 cut(s) 34, 214
BseGI GGATG 4 cut(s) 6, 29, 46, 234
BseMI GCAATG 1 cut(s) 63
BseMII CTCAG 2 cut(s) 269, 494
BseNI ACTGG 1 cut(s) 317
BseXI GCAGC 1 cut(s) 534
BsgI GTGCAG 1 cut(s) 70
BsiHKAI GWGCWC 1 cut(s) 297
BsiSI CCGG 2 cut(s) 50, 238
BsmAI GTCTC 3 cut(s) 170, 246, 491
BsmI GAATGC 1 cut(s) 380
Bso31I GGTCTC 2 cut(s) 170, 246
Bsp1286I GDGCHC 1 cut(s) 297
Bsp13I TCCGGA 1 cut(s) 49
Bsp143I GATC 3 cut(s) 140, 190, 440
BspACI CCGC 1 cut(s) 557
BspCNI CTCAG 2 cut(s) 268, 495
BspEI TCCGGA 1 cut(s) 49
BspMAI CTGCAG 1 cut(s) 204
BspPI GGATC 2 cut(s) 135, 185
BspQI GCTCTTC 1 cut(s) 285
BspTNI GGTCTC 2 cut(s) 170, 246
BsrDI GCAATG 1 cut(s) 63
BsrI ACTGG 1 cut(s) 317
BssECI CCNNGG 2 cut(s) 34, 214
BssMI GATC 3 cut(s) 140, 190, 440
BssT1I CCWWGG 1 cut(s) 214
Bst2UI CCWGG 2 cut(s) 36, 464
Bst4CI ACNGT 3 cut(s) 97, 129, 314
Bst6I CTCTTC 2 cut(s) 260, 285
BstAPI GCANNNNNTGC 2 cut(s) 284, 490
BstDEI CTNAG 3 cut(s) 255, 353, 503
BstF5I GGATG 4 cut(s) 6, 29, 46, 234
BstHHI GCGC 1 cut(s) 552
BstKTI GATC 3 cut(s) 143, 193, 443
BstMAI GTCTC 3 cut(s) 170, 246, 491
BstMBI GATC 3 cut(s) 140, 190, 440
BstMWI GCNNNNNNNGC 4 cut(s) 74, 284, 435, 490
BstNI CCWGG 2 cut(s) 36, 464
BstNSI RCATGY 1 cut(s) 547
BstSCI CCNGG 2 cut(s) 34, 462
BstSFI CTRYAG 1 cut(s) 200
BstV1I GCAGC 1 cut(s) 534
BstX2I RGATCY 1 cut(s) 140
BstYI RGATCY 1 cut(s) 140
BtrI CACGTC 1 cut(s) 584
BtsCI GGATG 4 cut(s) 6, 29, 46, 234
BtsIMutI CAGTG 1 cut(s) 500
CfoI GCGC 1 cut(s) 552
Csp6I GTAC 1 cut(s) 162
CspCI CAANNNNNGTGG 2 cut(s) 374, 409
CviAII CATG 2 cut(s) 377, 544
CviJI RGCY 4 cut(s) 61, 213, 352, 416
CviKI_1 RGCY 4 cut(s) 61, 213, 352, 416
CviQI GTAC 1 cut(s) 162
DdeI CTNAG 3 cut(s) 255, 353, 503
DpnI GATC 3 cut(s) 142, 192, 442
DpnII GATC 3 cut(s) 140, 190, 440
Eam1104I CTCTTC 2 cut(s) 260, 285
EarI CTCTTC 2 cut(s) 260, 285
Eco130I CCWWGG 1 cut(s) 214
Eco31I GGTCTC 2 cut(s) 170, 246
Eco57I CTGAAG 2 cut(s) 88, 152
EcoRII CCWGG 2 cut(s) 34, 462
EcoT14I CCWWGG 1 cut(s) 214
EcoT22I ATGCAT 1 cut(s) 382
ErhI CCWWGG 1 cut(s) 214
FaeI CATG 2 cut(s) 380, 547
FaiI YATR 8 cut(s) 251, 322, 378, 389, 436, 453, 482, 545
FatI CATG 2 cut(s) 376, 543
Fnu4HI GCNGC 1 cut(s) 548
FokI GGATG 3 cut(s) 16, 33, 221
Fsp4HI GCNGC 1 cut(s) 548
FspBI CTAG 2 cut(s) 221, 494
GlaI GCGC 1 cut(s) 551
GluI GCNGC 1 cut(s) 548
HapII CCGG 2 cut(s) 50, 238
HhaI GCGC 1 cut(s) 552
Hin1II CATG 2 cut(s) 380, 547
Hin6I GCGC 1 cut(s) 550
HinP1I GCGC 1 cut(s) 550
HinfI GANTC 2 cut(s) 53, 594
HpaII CCGG 2 cut(s) 50, 238
HphI GGTGA 2 cut(s) 341, 564
Hpy188I TCNGA 2 cut(s) 171, 445
Hpy188III TCNNGA 1 cut(s) 50
Hpy99I CGWCG 1 cut(s) 605
HpyAV CCTTC 3 cut(s) 21, 176, 335
HpyCH4III ACNGT 3 cut(s) 97, 129, 314
HpyCH4IV ACGT 3 cut(s) 160, 583, 609
HpyF10VI GCNNNNNNNGC 4 cut(s) 74, 284, 435, 490
HpyF3I CTNAG 3 cut(s) 255, 353, 503
HpySE526I ACGT 3 cut(s) 160, 583, 609
Hsp92II CATG 2 cut(s) 380, 547
HspAI GCGC 1 cut(s) 550
Kpn2I TCCGGA 1 cut(s) 49
Kzo9I GATC 3 cut(s) 140, 190, 440
LguI GCTCTTC 1 cut(s) 285
LpnPI CCDG 8 cut(s) 21, 48, 63, 251, 311, 330, 449, 476
Lsp1109I GCAGC 1 cut(s) 534
LweI GCATC 2 cut(s) 274, 405
MaeI CTAG 2 cut(s) 221, 494
MaeII ACGT 3 cut(s) 160, 583, 609
MaeIII GTNAC 1 cut(s) 531
MalI GATC 3 cut(s) 142, 192, 442
MboI GATC 3 cut(s) 140, 190, 440
MboII GAAGA 6 cut(s) 94, 277, 293, 302, 350, 533
MflI RGATCY 1 cut(s) 140
MhlI GDGCHC 1 cut(s) 297
MluCI AATT 2 cut(s) 332, 424
MnlI CCTC 3 cut(s) 30, 92, 498
Mph1103I ATGCAT 1 cut(s) 382
MroI TCCGGA 1 cut(s) 49
MseI TTAA 1 cut(s) 261
MspI CCGG 2 cut(s) 50, 238
MspR9I CCNGG 2 cut(s) 36, 464
Mva1269I GAATGC 1 cut(s) 380
MvaI CCWGG 2 cut(s) 36, 464
MwoI GCNNNNNNNGC 4 cut(s) 74, 284, 435, 490
NdeII GATC 3 cut(s) 140, 190, 440
NlaIII CATG 2 cut(s) 380, 547
NsiI ATGCAT 1 cut(s) 382
NspI RCATGY 1 cut(s) 547
PciSI GCTCTTC 1 cut(s) 285
PctI GAATGC 1 cut(s) 380
PfeI GAWTC 2 cut(s) 53, 594
PfoI TCCNGGA 1 cut(s) 462
PkrI GCNGC 1 cut(s) 549
Psp6I CCWGG 2 cut(s) 34, 462
PspGI CCWGG 2 cut(s) 34, 462
PstI CTGCAG 1 cut(s) 204
PsuI RGATCY 1 cut(s) 140
RsaI GTAC 1 cut(s) 163
RsaNI GTAC 1 cut(s) 162
SapI GCTCTTC 1 cut(s) 285
SaqAI TTAA 1 cut(s) 261
SatI GCNGC 1 cut(s) 548
Sau3AI GATC 3 cut(s) 140, 190, 440
ScrFI CCNGG 2 cut(s) 36, 464
SduI GDGCHC 1 cut(s) 297
SetI ASST 4 cut(s) 40, 163, 586, 612
SfaNI GCATC 2 cut(s) 274, 405
SfcI CTRYAG 1 cut(s) 200
Sse9I AATT 2 cut(s) 332, 424
SsiI CCGC 1 cut(s) 557
SspMI CTAG 2 cut(s) 221, 494
StyD4I CCNGG 2 cut(s) 34, 462
StyI CCWWGG 1 cut(s) 214
TaaI ACNGT 3 cut(s) 97, 129, 314
TaiI ACGT 3 cut(s) 163, 586, 612
TaqI TCGA 1 cut(s) 399
TasI AATT 2 cut(s) 332, 424
TfiI GAWTC 2 cut(s) 53, 594
Tru1I TTAA 1 cut(s) 261
Tru9I TTAA 1 cut(s) 261
TscAI CASTG 1 cut(s) 507
TseI GCWGC 1 cut(s) 547
TspRI CASTG 1 cut(s) 507
XapI RAATTY 1 cut(s) 332
XceI RCATGY 1 cut(s) 547
XspI CTAG 2 cut(s) 221, 494
Zsp2I ATGCAT 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.