FvH4_3g45220

Phytoene synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0016301
Physical Location & Seq
Reverse (-)
37578758 .. 37580820
2063 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g45220.t2

Sequence Viewer

Length: 1143 bp
ATGAGTTCAACATTTTCACTTGCACCAAAGCCTTACATTAGAGAGAGCAATGGAAATCTCCTGCCCCCAAAATCTATGCTCACAACAATAAGAGCTGAAGTGATTACAGCTCCCAAGCAAAGAAACACACGTAGTTTTCCTGAACTATCAATACAAGGCATTCCTGTTGCTGATTTGCATGTACAAGAAATTGTTCAGAGGCAATCCAAGAGCATAGCCAAGGTAGGTACTAGCCGAAAGTCACAGTTTGATCCAAGCTTTCTTGAAGAAGCATATCAAAGGTGCAGGAACATTTGTGCAGAATACGCCAAGACCTTCTATTTAGGTACTTTGCTAATGACAGAGGAGCGTCAAAAGGCAATATGGGCAATCTATGTTTGGTGTAGAAGAACAGATGAACTGGTTGATGGCCCCAATTCTGGGCACATGAACTCGGAAGTTCTTGATAGCTGGGAAGAGAGACTAGAAGACATTTTCGAAGGACGGCCTTATGACATGCTTGATGCTGCATTGACTCATACTGTTCTCAGCTTCCCCTTAGACATAAAGCCTTTTAGAGACATGATCGAGGGTATGCGAATGGACACAAGAAAATGCCGATATCAGAATTTTCAAGAGCTTTATCTCTACTGCTACTATGTAGCTGGAACTGTTGGCCTAATGAGCGTTCCTGTAATGGGAATTGCACCTGATTCTCTGAATTCTACTCAAAGTACATATGATGCTGCACTCTACTTGGGTATAGGGAATCAACTCACAAACATACTTAGAGATGTTGGAGAGGATGCTGCAAGAGGTAGAGTTTATCTTCCTCAAGATGAGCTTGCACAGTTTGGGTTATGCGACAACGATGTTTTTGCAAGAAAGGTCACTGATCAATGGAGAAAGTTCATGAAAGAACAGATTTCTAGAGCCAGATTCTATTTCAACCTGGCAGAAGAAGGTGCTTCTCAACTTGACAAAGATAGCCGTTGGCCGGTATGGTCATCCTTGCTGATATACCGAAACATCTTGGACGCAATTGAGGAAAATGACTACGATAACTTGACAAAGAGAGCATATGTAGGGAGAGCTAAGAAACTTCTCATGTTGCCTATGGCTTACACAAAATCTCTTCATACACCTAGTTTGATCTCTCAGTAG

Protein Analysis

381

Amino Acids

43.66

Weight (kDa)

6.18

Isoelectric Point (pI)

49.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQS_PSY PF00494 14 - 263 5.5e-69 Squalene/phytoene synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016301)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g45220
malus_domestica MD03G1007200.v1.1 MD11G1010500.v1.1
prunus_persica Prupe.6G006800_v2.0.a1
pyrus_communis pycom03g00730
rosa_chinensis RchiOBHm_Chr5g0082831
rosa_laevigata RLG00000036993
rosa_multiflora Rmu_sc0001381.1_g000014
rosa_roxburghii Rroxscaffold_1G00001600
rosa_rugosa Rorug05G0480200
rosa_samantha Rh5AG530500 Rh5BG557400 Rh5CG580400 Rh5DG565800
rosa_wichuraiana Rw5G049810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 245
AcoI YGGCCR 1 cut(s) 974
AcsI RAATTY 2 cut(s) 607, 700
AcuI CTGAAG 1 cut(s) 117
AfaI GTAC 4 cut(s) 183, 229, 328, 715
AfiI CCNNNNNNNGG 4 cut(s) 419, 420, 677, 976
AflIII ACRYGT 1 cut(s) 128
AgsI TTSAA 4 cut(s) 9, 266, 614, 928
AjnI CCWGG 1 cut(s) 930
AluBI AGCT 9 cut(s) 95, 110, 258, 450, 531, 619, 644, 823, 1073
AluI AGCT 9 cut(s) 95, 110, 258, 450, 531, 619, 644, 823, 1073
Alw26I GTCTC 2 cut(s) 454, 552
AlwI GGATC 1 cut(s) 245
AoxI GGCC 4 cut(s) 409, 485, 655, 974
ApeKI GCWGC 3 cut(s) 506, 725, 788
ApoI RAATTY 2 cut(s) 607, 700
Asp700I GAANNNNTTC 1 cut(s) 192
AspS9I GGNCC 1 cut(s) 410
AsuII TTCGAA 1 cut(s) 477
BaeGI GKGCMC 1 cut(s) 426
BbsI GAAGAC 1 cut(s) 474
BbvI GCAGC 3 cut(s) 493, 712, 775
BccI CCATC 1 cut(s) 401
BceAI ACGGC 2 cut(s) 500, 954
BcgI CGANNNNNNTGC 2 cut(s) 839, 873
BciT130I CCWGG 1 cut(s) 932
BclI TGATCA 1 cut(s) 874
BcoDI GTCTC 2 cut(s) 454, 552
BfaI CTAG 4 cut(s) 231, 464, 909, 1125
BisI GCNGC 3 cut(s) 507, 726, 789
BlsI GCNGC 3 cut(s) 508, 727, 790
Bme1390I CCNGG 1 cut(s) 932
BmgT120I GGNCC 1 cut(s) 410
BmiI GGNNCC 1 cut(s) 412
BmrFI CCNGG 1 cut(s) 932
BmsI GCATC 3 cut(s) 493, 712, 775
BpiI GAAGAC 1 cut(s) 474
Bpu14I TTCGAA 1 cut(s) 477
BpuEI CTTGAG 1 cut(s) 798
BsaAI YACGTR 1 cut(s) 131
BsaJI CCNNGG 1 cut(s) 219
Bsc4I CCNNNNNNNGG 4 cut(s) 419, 420, 677, 976
Bse118I RCCGGY 1 cut(s) 976
Bse1I ACTGG 1 cut(s) 405
Bse3DI GCAATG 1 cut(s) 55
BseBI CCWGG 1 cut(s) 932
BseDI CCNNGG 1 cut(s) 219
BseGI GGATG 2 cut(s) 790, 986
BseLI CCNNNNNNNGG 4 cut(s) 419, 420, 677, 976
BseMI GCAATG 1 cut(s) 55
BseMII CTCAG 1 cut(s) 541
BseNI ACTGG 1 cut(s) 405
BseRI GAGGAG 1 cut(s) 359
BseSI GKGCMC 1 cut(s) 426
BseXI GCAGC 3 cut(s) 493, 712, 775
BseYI CCCAGC 1 cut(s) 450
BsgI GTGCAG 3 cut(s) 304, 318, 711
BshFI GGCC 4 cut(s) 411, 487, 657, 976
BsiSI CCGG 1 cut(s) 977
BslI CCNNNNNNNGG 4 cut(s) 419, 420, 677, 976
BsmAI GTCTC 2 cut(s) 454, 552
BsmI GAATGC 1 cut(s) 159
BsnI GGCC 4 cut(s) 411, 487, 657, 976
Bsp119I TTCGAA 1 cut(s) 477
Bsp1286I GDGCHC 1 cut(s) 426
Bsp1407I TGTACA 1 cut(s) 181
Bsp143I GATC 4 cut(s) 250, 564, 874, 1131
BspANI GGCC 4 cut(s) 411, 487, 657, 976
BspCNI CTCAG 1 cut(s) 540
BspHI TCATGA 1 cut(s) 891
BspLI GGNNCC 1 cut(s) 412
BspPI GGATC 1 cut(s) 245
BspT104I TTCGAA 1 cut(s) 477
BsrDI GCAATG 1 cut(s) 55
BsrFI RCCGGY 1 cut(s) 976
BsrGI TGTACA 1 cut(s) 181
BsrI ACTGG 1 cut(s) 405
BssAI RCCGGY 1 cut(s) 976
BssECI CCNNGG 1 cut(s) 219
BssMI GATC 4 cut(s) 250, 564, 874, 1131
BssT1I CCWWGG 1 cut(s) 219
Bst2UI CCWGG 1 cut(s) 932
Bst4CI ACNGT 4 cut(s) 246, 523, 652, 831
Bst6I CTCTTC 2 cut(s) 450, 1119
BstAUI TGTACA 1 cut(s) 181
BstBAI YACGTR 1 cut(s) 131
BstBI TTCGAA 1 cut(s) 477
BstC8I GCNNGC 1 cut(s) 825
BstDEI CTNAG 5 cut(s) 527, 538, 767, 1074, 1137
BstF5I GGATG 2 cut(s) 790, 986
BstKTI GATC 4 cut(s) 253, 567, 877, 1134
BstMAI GTCTC 2 cut(s) 454, 552
BstMBI GATC 4 cut(s) 250, 564, 874, 1131
BstMWI GCNNNNNNNGC 3 cut(s) 305, 365, 663
BstNI CCWGG 1 cut(s) 932
BstNSI RCATGY 2 cut(s) 182, 499
BstSCI CCNGG 1 cut(s) 930
BstSLI GKGCMC 1 cut(s) 426
BstV1I GCAGC 3 cut(s) 493, 712, 775
BstV2I GAAGAC 1 cut(s) 474
BsuRI GGCC 4 cut(s) 411, 487, 657, 976
BtsCI GGATG 2 cut(s) 790, 986
BtsIMutI CAGTG 1 cut(s) 870
Cac8I GCNNGC 1 cut(s) 825
CciI TCATGA 1 cut(s) 891
Cfr10I RCCGGY 1 cut(s) 976
Cfr13I GGNCC 1 cut(s) 410
CseI GACGC 2 cut(s) 338, 1025
Csp6I GTAC 4 cut(s) 182, 228, 327, 714
CviAII CATG 6 cut(s) 179, 427, 496, 562, 892, 1087
CviQI GTAC 4 cut(s) 182, 228, 327, 714
DdeI CTNAG 5 cut(s) 527, 538, 767, 1074, 1137
DpnI GATC 4 cut(s) 252, 566, 876, 1133
DpnII GATC 4 cut(s) 250, 564, 874, 1131
EaeI YGGCCR 1 cut(s) 974
Eam1104I CTCTTC 2 cut(s) 450, 1119
EarI CTCTTC 2 cut(s) 450, 1119
Eco130I CCWWGG 1 cut(s) 219
Eco32I GATATC 1 cut(s) 602
Eco57I CTGAAG 1 cut(s) 117
EcoRI GAATTC 1 cut(s) 700
EcoRII CCWGG 1 cut(s) 930
EcoRV GATATC 1 cut(s) 602
EcoT14I CCWWGG 1 cut(s) 219
ErhI CCWWGG 1 cut(s) 219
FaeI CATG 6 cut(s) 182, 430, 499, 565, 895, 1090
FalI AAGNNNNNCTT 2 cut(s) 807, 839
FatI CATG 6 cut(s) 178, 426, 495, 561, 891, 1086
FauNDI CATATG 2 cut(s) 718, 1060
FbaI TGATCA 1 cut(s) 874
Fnu4HI GCNGC 3 cut(s) 507, 726, 789
FokI GGATG 2 cut(s) 797, 973
Fsp4HI GCNGC 3 cut(s) 507, 726, 789
FspBI CTAG 4 cut(s) 231, 464, 909, 1125
GluI GCNGC 3 cut(s) 507, 726, 789
GsaI CCCAGC 1 cut(s) 454
HaeIII GGCC 4 cut(s) 411, 487, 657, 976
HapII CCGG 1 cut(s) 977
HgaI GACGC 2 cut(s) 338, 1025
Hin1II CATG 6 cut(s) 182, 430, 499, 565, 895, 1090
HindIII AAGCTT 1 cut(s) 256
HinfI GANTC 4 cut(s) 514, 692, 748, 918
HpaII CCGG 1 cut(s) 977
Hpy188I TCNGA 4 cut(s) 198, 436, 606, 699
Hpy188III TCNNGA 7 cut(s) 140, 263, 443, 614, 815, 892, 909
HpyAV CCTTC 3 cut(s) 325, 473, 935
HpyCH4III ACNGT 4 cut(s) 246, 523, 652, 831
HpyCH4IV ACGT 1 cut(s) 130
HpyF10VI GCNNNNNNNGC 3 cut(s) 305, 365, 663
HpyF3I CTNAG 5 cut(s) 527, 538, 767, 1074, 1137
HpySE526I ACGT 1 cut(s) 130
Hsp92II CATG 6 cut(s) 182, 430, 499, 565, 895, 1090
Ksp22I TGATCA 1 cut(s) 874
Kzo9I GATC 4 cut(s) 250, 564, 874, 1131
LmnI GCTCC 2 cut(s) 115, 346
Lsp1109I GCAGC 3 cut(s) 493, 712, 775
LweI GCATC 3 cut(s) 493, 712, 775
MaeI CTAG 4 cut(s) 231, 464, 909, 1125
MaeII ACGT 1 cut(s) 130
MaeIII GTNAC 2 cut(s) 240, 868
MalI GATC 4 cut(s) 252, 566, 876, 1133
MboI GATC 4 cut(s) 250, 564, 874, 1131
MboII GAAGA 7 cut(s) 278, 399, 467, 479, 800, 950, 1106
MfeI CAATTG 1 cut(s) 1020
MhlI GDGCHC 1 cut(s) 426
MluCI AATT 6 cut(s) 189, 415, 607, 681, 700, 1020
MlyI GAGTC 1 cut(s) 508
MmeI TCCRAC 1 cut(s) 757
MnlI CCTC 7 cut(s) 192, 337, 562, 775, 788, 822, 1018
MroXI GAANNNNTTC 1 cut(s) 192
MspI CCGG 1 cut(s) 977
MspR9I CCNGG 1 cut(s) 932
MunI CAATTG 1 cut(s) 1020
Mva1269I GAATGC 1 cut(s) 159
MvaI CCWGG 1 cut(s) 932
MwoI GCNNNNNNNGC 3 cut(s) 305, 365, 663
NdeI CATATG 2 cut(s) 718, 1060
NdeII GATC 4 cut(s) 250, 564, 874, 1131
NlaIII CATG 6 cut(s) 182, 430, 499, 565, 895, 1090
NlaIV GGNNCC 1 cut(s) 412
NmuCI GTSAC 2 cut(s) 240, 868
NspI RCATGY 2 cut(s) 182, 499
NspV TTCGAA 1 cut(s) 477
PagI TCATGA 1 cut(s) 891
PctI GAATGC 1 cut(s) 159
PdmI GAANNNNTTC 1 cut(s) 192
PfeI GAWTC 3 cut(s) 692, 748, 918
PkrI GCNGC 3 cut(s) 508, 727, 790
PleI GAGTC 1 cut(s) 508
PpsI GAGTC 1 cut(s) 508
Ppu21I YACGTR 1 cut(s) 131
Psp6I CCWGG 1 cut(s) 930
PspFI CCCAGC 1 cut(s) 450
PspGI CCWGG 1 cut(s) 930
PspN4I GGNNCC 1 cut(s) 412
PspPI GGNCC 1 cut(s) 410
PsrI GAACNNNNNNTAC 2 cut(s) 135, 167
RsaI GTAC 4 cut(s) 183, 229, 328, 715
RsaNI GTAC 4 cut(s) 182, 228, 327, 714
SatI GCNGC 3 cut(s) 507, 726, 789
Sau3AI GATC 4 cut(s) 250, 564, 874, 1131
Sau96I GGNCC 1 cut(s) 410
SchI GAGTC 1 cut(s) 508
ScrFI CCNGG 1 cut(s) 932
SduI GDGCHC 1 cut(s) 426
SfaNI GCATC 3 cut(s) 493, 712, 775
SfuI TTCGAA 1 cut(s) 477
SmlI CTYRAG 1 cut(s) 813
SmoI CTYRAG 1 cut(s) 813
Sse9I AATT 6 cut(s) 189, 415, 607, 681, 700, 1020
SspMI CTAG 4 cut(s) 231, 464, 909, 1125
StyD4I CCNGG 1 cut(s) 930
StyI CCWWGG 1 cut(s) 219
TaaI ACNGT 4 cut(s) 246, 523, 652, 831
TaiI ACGT 1 cut(s) 133
TaqI TCGA 2 cut(s) 477, 567
TasI AATT 6 cut(s) 189, 415, 607, 681, 700, 1020
TatI WGTACW 2 cut(s) 181, 713
TfiI GAWTC 3 cut(s) 692, 748, 918
TscAI CASTG 1 cut(s) 877
TseFI GTSAC 2 cut(s) 240, 868
TseI GCWGC 3 cut(s) 506, 725, 788
Tsp45I GTSAC 2 cut(s) 240, 868
TspDTI ATGAA 5 cut(s) 411, 443, 880, 908, 1106
TspRI CASTG 1 cut(s) 877
XapI RAATTY 2 cut(s) 607, 700
XbaI TCTAGA 1 cut(s) 908
XceI RCATGY 2 cut(s) 182, 499
XmnI GAANNNNTTC 1 cut(s) 192
XspI CTAG 4 cut(s) 231, 464, 909, 1125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.