Rorug05G0480200

Phytoene synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0016301
Physical Location & Seq
Forward (+)
66143145 .. 66143961
817 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0480200.1

Sequence Viewer

Length: 489 bp
ATGGCGCAAATTATAGTGTGCTCATGTCCTATATCAGCTCCACAAAAGACCCTCGAGCAAATAAATATATTGAAAAGTGAAGTCATAAAAGCTGCTGCACGCCTGCACCTATACTCACTCCCTTCTCTTCATGTGGACCGAATACAGCCAGACATTAGTGCCCCGGGGGCACAAATATTGGCTGCATATTCACCTGTTGCGCCTATCTCATCTATTCCTGTACCTGGCGACTTTAGGCGTGTAAAATACAATGTACAATCTGGAACCTCCATGGCTTGCCCCCACGCTGCTGGTGCAGCTGCATATGTTAAAACAATCCACCCTGATTGGTCTCCGGCAGCCATCAAATCATCTCTTATGACTACTGCTTGGTCCATGAATAGTACCATTACTGACCCTTCTGGTGAATTCGGCTACGGAAGCGGACATATCAATCCTGTAGAAGCTTTAAATCCAGGGCTTGTGTATGAAGCTTCCAAAGAAGATTAG

Protein Analysis

162

Amino Acids

17.16

Weight (kDa)

6.5

Isoelectric Point (pI)

50.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 41 - 140 2.8e-17 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016301)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g45220
malus_domestica MD03G1007200.v1.1 MD11G1010500.v1.1
prunus_persica Prupe.6G006800_v2.0.a1
pyrus_communis pycom03g00730
rosa_chinensis RchiOBHm_Chr5g0082831
rosa_laevigata RLG00000036993
rosa_multiflora Rmu_sc0001381.1_g000014
rosa_roxburghii Rroxscaffold_1G00001600
rosa_rugosa Rorug05G0480200
rosa_samantha Rh5AG530500 Rh5BG557400 Rh5CG580400 Rh5DG565800
rosa_wichuraiana Rw5G049810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 423
AcsI RAATTY 1 cut(s) 407
AfaI GTAC 3 cut(s) 222, 255, 385
AfiI CCNNNNNNNGG 1 cut(s) 224
AgsI TTSAA 1 cut(s) 73
AjnI CCWGG 2 cut(s) 223, 454
AluBI AGCT 5 cut(s) 38, 92, 299, 446, 473
AluI AGCT 5 cut(s) 38, 92, 299, 446, 473
Alw21I GWGCWC 1 cut(s) 23
Alw26I GTCTC 1 cut(s) 336
Ama87I CYCGRG 2 cut(s) 53, 163
ApeKI GCWGC 7 cut(s) 92, 95, 182, 287, 296, 299, 338
ApoI RAATTY 1 cut(s) 407
AspLEI GCGC 2 cut(s) 7, 202
AspS9I GGNCC 2 cut(s) 136, 372
AsuC2I CCSGG 2 cut(s) 164, 165
AsuHPI GGTGA 2 cut(s) 183, 416
AvaI CYCGRG 2 cut(s) 53, 163
AvaII GGWCC 2 cut(s) 136, 372
BaeGI GKGCMC 2 cut(s) 163, 172
Bbv12I GWGCWC 1 cut(s) 23
BbvI GCAGC 7 cut(s) 79, 82, 169, 274, 286, 308, 350
BccI CCATC 1 cut(s) 350
BciT130I CCWGG 2 cut(s) 225, 456
BcnI CCSGG 2 cut(s) 164, 165
BcoDI GTCTC 1 cut(s) 336
BfmI CTRYAG 1 cut(s) 438
BglI GCCNNNNNGGC 1 cut(s) 167
BisI GCNGC 7 cut(s) 93, 96, 183, 288, 297, 300, 339
BlsI GCNGC 7 cut(s) 94, 97, 184, 289, 298, 301, 340
Bme1390I CCNGG 4 cut(s) 164, 165, 225, 456
Bme18I GGWCC 2 cut(s) 136, 372
BmeT110I CYCGRG 2 cut(s) 53, 163
BmgT120I GGNCC 2 cut(s) 136, 372
BmiI GGNNCC 1 cut(s) 265
BmrFI CCNGG 4 cut(s) 164, 165, 225, 456
BpuMI CCSGG 2 cut(s) 164, 165
BsaI GGTCTC 1 cut(s) 336
BsaJI CCNNGG 5 cut(s) 162, 163, 164, 270, 455
Bsc4I CCNNNNNNNGG 1 cut(s) 224
BseBI CCWGG 2 cut(s) 225, 456
BseDI CCNNGG 5 cut(s) 162, 163, 164, 270, 455
BseLI CCNNNNNNNGG 1 cut(s) 224
BseSI GKGCMC 2 cut(s) 163, 172
BseXI GCAGC 7 cut(s) 79, 82, 169, 274, 286, 308, 350
BsgI GTGCAG 3 cut(s) 81, 89, 315
BsiHKAI GWGCWC 1 cut(s) 23
BsiHKCI CYCGRG 2 cut(s) 53, 163
BsiSI CCGG 2 cut(s) 164, 335
BslI CCNNNNNNNGG 1 cut(s) 224
BsmAI GTCTC 1 cut(s) 336
Bso31I GGTCTC 1 cut(s) 336
BsoBI CYCGRG 2 cut(s) 53, 163
Bsp1286I GDGCHC 3 cut(s) 23, 163, 172
Bsp1407I TGTACA 1 cut(s) 253
Bsp19I CCATGG 1 cut(s) 270
BspACI CCGC 1 cut(s) 423
BspLI GGNNCC 1 cut(s) 265
BspTNI GGTCTC 1 cut(s) 336
BsrGI TGTACA 1 cut(s) 253
BssECI CCNNGG 5 cut(s) 162, 163, 164, 270, 455
BssT1I CCWWGG 1 cut(s) 270
Bst2UI CCWGG 2 cut(s) 225, 456
Bst6I CTCTTC 1 cut(s) 132
BstAUI TGTACA 1 cut(s) 253
BstC8I GCNNGC 3 cut(s) 100, 104, 277
BstDSI CCRYGG 1 cut(s) 270
BstHHI GCGC 2 cut(s) 7, 202
BstMAI GTCTC 1 cut(s) 336
BstMWI GCNNNNNNNGC 4 cut(s) 167, 293, 296, 420
BstNI CCWGG 2 cut(s) 225, 456
BstSCI CCNGG 4 cut(s) 162, 163, 223, 454
BstSFI CTRYAG 1 cut(s) 438
BstSLI GKGCMC 2 cut(s) 163, 172
BstV1I GCAGC 7 cut(s) 79, 82, 169, 274, 286, 308, 350
BstXI CCANNNNNNTGG 1 cut(s) 290
BtgI CCRYGG 1 cut(s) 270
Cac8I GCNNGC 3 cut(s) 100, 104, 277
CfoI GCGC 2 cut(s) 7, 202
Cfr13I GGNCC 2 cut(s) 136, 372
Cfr9I CCCGGG 1 cut(s) 163
Csp6I GTAC 3 cut(s) 221, 254, 384
CspCI CAANNNNNGTGG 2 cut(s) 308, 343
CviAII CATG 4 cut(s) 24, 131, 271, 376
CviQI GTAC 3 cut(s) 221, 254, 384
DraI TTTAAA 1 cut(s) 450
Eam1104I CTCTTC 1 cut(s) 132
EarI CTCTTC 1 cut(s) 132
Eco130I CCWWGG 1 cut(s) 270
Eco31I GGTCTC 1 cut(s) 336
Eco47I GGWCC 2 cut(s) 136, 372
Eco88I CYCGRG 2 cut(s) 53, 163
EcoRI GAATTC 1 cut(s) 407
EcoRII CCWGG 2 cut(s) 223, 454
EcoT14I CCWWGG 1 cut(s) 270
ErhI CCWWGG 1 cut(s) 270
FaeI CATG 4 cut(s) 27, 134, 274, 379
FatI CATG 4 cut(s) 23, 130, 270, 375
FauNDI CATATG 1 cut(s) 304
Fnu4HI GCNGC 7 cut(s) 93, 96, 183, 288, 297, 300, 339
Fsp4HI GCNGC 7 cut(s) 93, 96, 183, 288, 297, 300, 339
GlaI GCGC 2 cut(s) 6, 201
GluI GCNGC 7 cut(s) 93, 96, 183, 288, 297, 300, 339
HapII CCGG 2 cut(s) 164, 335
HhaI GCGC 2 cut(s) 7, 202
Hin1II CATG 4 cut(s) 27, 134, 274, 379
Hin6I GCGC 2 cut(s) 5, 200
HinP1I GCGC 2 cut(s) 5, 200
HindIII AAGCTT 2 cut(s) 444, 471
HpaII CCGG 2 cut(s) 164, 335
HphI GGTGA 2 cut(s) 183, 416
Hpy166II GTNNAC 1 cut(s) 136
Hpy188III TCNNGA 1 cut(s) 261
Hpy8I GTNNAC 1 cut(s) 136
HpyAV CCTTC 2 cut(s) 132, 408
HpyCH4V TGCA 5 cut(s) 98, 106, 185, 296, 302
HpyF10VI GCNNNNNNNGC 4 cut(s) 167, 293, 296, 420
Hsp92II CATG 4 cut(s) 27, 134, 274, 379
HspAI GCGC 2 cut(s) 5, 200
LmnI GCTCC 1 cut(s) 43
Lsp1109I GCAGC 7 cut(s) 79, 82, 169, 274, 286, 308, 350
MboII GAAGA 1 cut(s) 119
MhlI GDGCHC 3 cut(s) 23, 163, 172
MluCI AATT 2 cut(s) 9, 407
MnlI CCTC 2 cut(s) 62, 277
MseI TTAA 2 cut(s) 309, 449
MspA1I CMGCKG 1 cut(s) 299
MspI CCGG 2 cut(s) 164, 335
MspR9I CCNGG 4 cut(s) 164, 165, 225, 456
MvaI CCWGG 2 cut(s) 225, 456
MwoI GCNNNNNNNGC 4 cut(s) 167, 293, 296, 420
NciI CCSGG 2 cut(s) 164, 165
NcoI CCATGG 1 cut(s) 270
NdeI CATATG 1 cut(s) 304
NlaIII CATG 4 cut(s) 27, 134, 274, 379
NlaIV GGNNCC 1 cut(s) 265
PaeR7I CTCGAG 1 cut(s) 53
PkrI GCNGC 7 cut(s) 94, 97, 184, 289, 298, 301, 340
Psp6I CCWGG 2 cut(s) 223, 454
PspGI CCWGG 2 cut(s) 223, 454
PspN4I GGNNCC 1 cut(s) 265
PspPI GGNCC 2 cut(s) 136, 372
PspXI VCTCGAGB 1 cut(s) 53
PvuII CAGCTG 1 cut(s) 299
RsaI GTAC 3 cut(s) 222, 255, 385
RsaNI GTAC 3 cut(s) 221, 254, 384
SaqAI TTAA 2 cut(s) 309, 449
SatI GCNGC 7 cut(s) 93, 96, 183, 288, 297, 300, 339
Sau96I GGNCC 2 cut(s) 136, 372
ScrFI CCNGG 4 cut(s) 164, 165, 225, 456
SduI GDGCHC 3 cut(s) 23, 163, 172
SetI ASST 9 cut(s) 40, 94, 111, 196, 226, 269, 301, 448, 475
SfcI CTRYAG 1 cut(s) 438
Sfr274I CTCGAG 1 cut(s) 53
SinI GGWCC 2 cut(s) 136, 372
SlaI CTCGAG 1 cut(s) 53
SmaI CCCGGG 1 cut(s) 165
SmlI CTYRAG 1 cut(s) 53
SmoI CTYRAG 1 cut(s) 53
Sse9I AATT 2 cut(s) 9, 407
SsiI CCGC 1 cut(s) 423
SspI AATATT 1 cut(s) 177
StyD4I CCNGG 4 cut(s) 162, 163, 223, 454
StyI CCWWGG 1 cut(s) 270
TaqI TCGA 1 cut(s) 54
TaqII GACCGA 1 cut(s) 153
TasI AATT 2 cut(s) 9, 407
TatI WGTACW 1 cut(s) 253
Tru1I TTAA 2 cut(s) 309, 449
Tru9I TTAA 2 cut(s) 309, 449
TseI GCWGC 7 cut(s) 92, 95, 182, 287, 296, 299, 338
TspDTI ATGAA 3 cut(s) 119, 392, 483
TspGWI ACGGA 1 cut(s) 432
TspMI CCCGGG 1 cut(s) 163
VpaK11BI GGWCC 2 cut(s) 136, 372
XapI RAATTY 1 cut(s) 407
XhoI CTCGAG 1 cut(s) 53
XmaI CCCGGG 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.