MD11G1010500.v1.1

Phytoene synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0016301
Physical Location & Seq
Forward (+)
792262 .. 794651
2390 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1010500.v1.1.491

Sequence Viewer

Length: 1149 bp
ATGTGTTCCACACTTTCCTTCGCAGGAAAGACTTACATTGGAGAGAGCAATGAAAGAATTCGGTGTCGAAAATCTATGGTCACAGCAGCAAAAGCTCAAGTGATTACAGCTCCCAAGCAAAGAAGCAGGCCTTTTTTTCCTCAGCTTTCAATTCAAGGCATTCCTCTTGCTGATTTACACGTGCAAGAAATCGTTCAAAGGCAGTCTCAGACTTATAGTGTGGACGGGGAAGGCGGCCGGCGCAGGCCGCAGTTTAACCCTAGCTTTCTTGAAGAAGCATATGAGAGGTGCAAGAACCTTTGTGCAGAATATGCCAAGACATTCTATCTAGGCACTTTACTTATGACAGAAGAACGTCAAAAGGCGATATGGGCAATCTATGTTTGGTGTAGGAGGACAGATGAACTGGTGGACGGCCCGAATTCCGGCTACATGACCTCAGAAGTTCTTGATAGATGGGAACAAAGATTGGAAGACATTTTCGAAGGACGGCCTTATGACATGCTTGATGCTGCATTGACTCATACTGTTTCCAATTTCCCCTTAGACATCAAGCCTTTTAGGGACATGATCGAGGGTATGAGAACGGACATAGTAAAATGTCGATACCAAAATTTTCAAGAGCTCTATCTTTACTGCTACTACGTGGCAGGGACGGTTGGCCTAATGAGTGTTCCTGTAATGGGAATTGCACCAGATTCTTTGATTTCTGCACAACGTACATATGATTCTGCATTATATTTGGGCATAGGAAACCAACTCACAAACATCCTTAGAGATGTTGGAGAGGATGCAATGAGAGGGAGAGTTTATCTTCCCCAAGACGAGCTTGCACAGTTTGGGTTATGCGATAACGATGTTTTTTCAAGAAAGGTGACTGATCAATGGAGAGCGTTCATGAAAGAACAAATTGCAAGAGCAAGGTTTTATTTCAACCTAGCAGAAGAAGGAGCTTCTCAGCTTGATAAGGCCAGCCGTTGGCCGGTATGGTCATCGTTACTGATATATCGAAACATACTGGACGCGATCGAAGACAACGATTACGATAATTTGACAAAGAGAGCATACGTAAAGAGAGCCAAGAAACTTCTCATGCTGCCTTTGGCATACACAAGATCTCTATCAACACATACTTTGATGTCCCAATAA

Protein Analysis

383

Amino Acids

44.08

Weight (kDa)

7.5

Isoelectric Point (pI)

50.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SQS_PSY PF00494 103 - 359 1.2e-70 Squalene/phytoene synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016301)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g45220
malus_domestica MD03G1007200.v1.1 MD11G1010500.v1.1
prunus_persica Prupe.6G006800_v2.0.a1
pyrus_communis pycom03g00730
rosa_chinensis RchiOBHm_Chr5g0082831
rosa_laevigata RLG00000036993
rosa_multiflora Rmu_sc0001381.1_g000014
rosa_roxburghii Rroxscaffold_1G00001600
rosa_rugosa Rorug05G0480200
rosa_samantha Rh5AG530500 Rh5BG557400 Rh5CG580400 Rh5DG565800
rosa_wichuraiana Rw5G049810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 978
AccII CGCG 1 cut(s) 1025
AciI CCGC 2 cut(s) 234, 248
AcoI YGGCCR 2 cut(s) 235, 980
AcsI RAATTY 3 cut(s) 57, 421, 613
AcvI CACGTG 1 cut(s) 181
AfaI GTAC 1 cut(s) 721
AfiI CCNNNNNNNGG 4 cut(s) 425, 683, 978, 982
AflIII ACRYGT 1 cut(s) 178
AgsI TTSAA 7 cut(s) 150, 155, 197, 272, 620, 867, 934
AluBI AGCT 8 cut(s) 95, 110, 145, 264, 625, 829, 953, 961
AluI AGCT 8 cut(s) 95, 110, 145, 264, 625, 829, 953, 961
Alw21I GWGCWC 1 cut(s) 627
Alw26I GTCTC 1 cut(s) 210
AoxI GGCC 8 cut(s) 128, 235, 245, 415, 491, 661, 969, 980
ApeKI GCWGC 3 cut(s) 86, 512, 1096
ApoI RAATTY 3 cut(s) 57, 421, 613
Asp700I GAANNNNTTC 2 cut(s) 57, 192
AspLEI GCGC 1 cut(s) 243
AspS9I GGNCC 1 cut(s) 416
AsuHPI GGTGA 1 cut(s) 886
AsuII TTCGAA 1 cut(s) 483
BanII GRGCYC 1 cut(s) 627
BbrPI CACGTG 1 cut(s) 181
BbsI GAAGAC 2 cut(s) 480, 1038
Bbv12I GWGCWC 1 cut(s) 627
BbvCI CCTCAGC 1 cut(s) 141
BbvI GCAGC 3 cut(s) 98, 499, 1083
BccI CCATC 1 cut(s) 450
BceAI ACGGC 3 cut(s) 430, 506, 960
BclI TGATCA 1 cut(s) 880
BcoDI GTCTC 1 cut(s) 210
BfaI CTAG 3 cut(s) 261, 329, 938
BglII AGATCT 1 cut(s) 1115
BisI GCNGC 5 cut(s) 87, 235, 248, 513, 1097
BlsI GCNGC 5 cut(s) 88, 236, 249, 514, 1098
BmgT120I GGNCC 1 cut(s) 416
BmsI GCATC 2 cut(s) 499, 781
BpiI GAAGAC 2 cut(s) 480, 1038
Bpu10I CCTNAGC 1 cut(s) 141
Bpu14I TTCGAA 1 cut(s) 483
BpuEI CTTGAG 1 cut(s) 81
BsaAI YACGTR 3 cut(s) 181, 646, 1069
BsaBI GATNNNNATC 1 cut(s) 1120
Bsc4I CCNNNNNNNGG 4 cut(s) 425, 683, 978, 982
Bse118I RCCGGY 2 cut(s) 237, 982
Bse1I ACTGG 2 cut(s) 411, 1023
Bse3DI GCAATG 2 cut(s) 55, 801
Bse8I GATNNNNATC 1 cut(s) 1120
BseGI GGATG 2 cut(s) 768, 796
BseJI GATNNNNATC 1 cut(s) 1120
BseLI CCNNNNNNNGG 4 cut(s) 425, 683, 978, 982
BseMI GCAATG 2 cut(s) 55, 801
BseMII CTCAG 4 cut(s) 155, 221, 453, 971
BseNI ACTGG 2 cut(s) 411, 1023
BseX3I CGGCCG 1 cut(s) 235
BseXI GCAGC 3 cut(s) 98, 499, 1083
BsgI GTGCAG 2 cut(s) 324, 696
Bsh1236I CGCG 1 cut(s) 1025
Bsh1285I CGRYCG 2 cut(s) 238, 1029
BshFI GGCC 8 cut(s) 130, 237, 247, 417, 493, 663, 971, 982
BsiEI CGRYCG 2 cut(s) 238, 1029
BsiHKAI GWGCWC 1 cut(s) 627
BsiSI CCGG 3 cut(s) 238, 426, 983
BslFI GGGAC 3 cut(s) 578, 667, 1126
BslI CCNNNNNNNGG 4 cut(s) 425, 683, 978, 982
BsmAI GTCTC 1 cut(s) 210
BsmFI GGGAC 3 cut(s) 578, 667, 1126
BsmI GAATGC 1 cut(s) 159
BsnI GGCC 8 cut(s) 130, 237, 247, 417, 493, 663, 971, 982
Bsp119I TTCGAA 1 cut(s) 483
Bsp1286I GDGCHC 1 cut(s) 627
Bsp143I GATC 4 cut(s) 570, 880, 1026, 1115
BspACI CCGC 2 cut(s) 234, 248
BspANI GGCC 8 cut(s) 130, 237, 247, 417, 493, 663, 971, 982
BspCNI CTCAG 4 cut(s) 154, 220, 452, 970
BspFNI CGCG 1 cut(s) 1025
BspHI TCATGA 1 cut(s) 897
BspT104I TTCGAA 1 cut(s) 483
BsrDI GCAATG 2 cut(s) 55, 801
BsrFI RCCGGY 2 cut(s) 237, 982
BsrI ACTGG 2 cut(s) 411, 1023
BssAI RCCGGY 2 cut(s) 237, 982
BssMI GATC 4 cut(s) 570, 880, 1026, 1115
Bst4CI ACNGT 3 cut(s) 529, 658, 837
BstAPI GCANNNNNTGC 1 cut(s) 311
BstBAI YACGTR 3 cut(s) 181, 646, 1069
BstBI TTCGAA 1 cut(s) 483
BstC8I GCNNGC 5 cut(s) 128, 239, 245, 831, 973
BstDEI CTNAG 6 cut(s) 141, 207, 439, 544, 773, 957
BstF5I GGATG 2 cut(s) 768, 796
BstFNI CGCG 1 cut(s) 1025
BstHHI GCGC 1 cut(s) 243
BstKTI GATC 4 cut(s) 573, 883, 1029, 1118
BstMAI GTCTC 1 cut(s) 210
BstMBI GATC 4 cut(s) 570, 880, 1026, 1115
BstMCI CGRYCG 2 cut(s) 238, 1029
BstMWI GCNNNNNNNGC 5 cut(s) 92, 240, 247, 311, 371
BstNSI RCATGY 1 cut(s) 505
BstSNI TACGTA 1 cut(s) 1069
BstUI CGCG 1 cut(s) 1025
BstV1I GCAGC 3 cut(s) 98, 499, 1083
BstV2I GAAGAC 2 cut(s) 480, 1038
BstX2I RGATCY 1 cut(s) 1115
BstYI RGATCY 1 cut(s) 1115
BstZI CGGCCG 1 cut(s) 235
BsuRI GGCC 8 cut(s) 130, 237, 247, 417, 493, 663, 971, 982
BtsCI GGATG 2 cut(s) 768, 796
Cac8I GCNNGC 5 cut(s) 128, 239, 245, 831, 973
CciI TCATGA 1 cut(s) 897
CfoI GCGC 1 cut(s) 243
Cfr10I RCCGGY 2 cut(s) 237, 982
Cfr13I GGNCC 1 cut(s) 416
CseI GACGC 1 cut(s) 1031
Csp6I GTAC 1 cut(s) 720
CviAII CATG 5 cut(s) 433, 502, 568, 898, 1093
CviQI GTAC 1 cut(s) 720
DdeI CTNAG 6 cut(s) 141, 207, 439, 544, 773, 957
DpnI GATC 4 cut(s) 572, 882, 1028, 1117
DpnII GATC 4 cut(s) 570, 880, 1026, 1115
EaeI YGGCCR 2 cut(s) 235, 980
EagI CGGCCG 1 cut(s) 235
Ecl136II GAGCTC 1 cut(s) 625
EclXI CGGCCG 1 cut(s) 235
Eco105I TACGTA 1 cut(s) 1069
Eco147I AGGCCT 1 cut(s) 130
Eco24I GRGCYC 1 cut(s) 627
Eco52I CGGCCG 1 cut(s) 235
Eco53kI GAGCTC 1 cut(s) 625
Eco72I CACGTG 1 cut(s) 181
EcoICRI GAGCTC 1 cut(s) 625
EcoRI GAATTC 2 cut(s) 57, 421
EcoT38I GRGCYC 1 cut(s) 627
FaeI CATG 5 cut(s) 436, 505, 571, 901, 1096
FalI AAGNNNNNCTT 4 cut(s) 115, 147, 813, 845
FaqI GGGAC 3 cut(s) 578, 667, 1126
FatI CATG 5 cut(s) 432, 501, 567, 897, 1092
FauNDI CATATG 2 cut(s) 280, 724
FbaI TGATCA 1 cut(s) 880
Fnu4HI GCNGC 5 cut(s) 87, 235, 248, 513, 1097
FokI GGATG 2 cut(s) 755, 803
FriOI GRGCYC 1 cut(s) 627
Fsp4HI GCNGC 5 cut(s) 87, 235, 248, 513, 1097
FspBI CTAG 3 cut(s) 261, 329, 938
GlaI GCGC 1 cut(s) 242
GluI GCNGC 5 cut(s) 87, 235, 248, 513, 1097
HaeIII GGCC 8 cut(s) 130, 237, 247, 417, 493, 663, 971, 982
HapII CCGG 3 cut(s) 238, 426, 983
HgaI GACGC 1 cut(s) 1031
HhaI GCGC 1 cut(s) 243
Hin1II CATG 5 cut(s) 436, 505, 571, 901, 1096
Hin6I GCGC 1 cut(s) 241
HinP1I GCGC 1 cut(s) 241
HinfI GANTC 3 cut(s) 520, 698, 728
HpaII CCGG 3 cut(s) 238, 426, 983
HphI GGTGA 1 cut(s) 886
Hpy166II GTNNAC 2 cut(s) 223, 412
Hpy188I TCNGA 2 cut(s) 210, 442
Hpy188III TCNNGA 5 cut(s) 269, 449, 620, 867, 898
Hpy8I GTNNAC 2 cut(s) 223, 412
HpyAV CCTTC 4 cut(s) 28, 224, 479, 941
HpyCH4III ACNGT 3 cut(s) 529, 658, 837
HpyCH4IV ACGT 5 cut(s) 180, 355, 645, 718, 1068
HpyF10VI GCNNNNNNNGC 5 cut(s) 92, 240, 247, 311, 371
HpyF3I CTNAG 6 cut(s) 141, 207, 439, 544, 773, 957
HpySE526I ACGT 5 cut(s) 180, 355, 645, 718, 1068
Hsp92II CATG 5 cut(s) 436, 505, 571, 901, 1096
HspAI GCGC 1 cut(s) 241
KroI GCCGGC 1 cut(s) 237
KroNI GCCGGC 1 cut(s) 239
Ksp22I TGATCA 1 cut(s) 880
Kzo9I GATC 4 cut(s) 570, 880, 1026, 1115
LmnI GCTCC 2 cut(s) 115, 950
Lsp1109I GCAGC 3 cut(s) 98, 499, 1083
LweI GCATC 2 cut(s) 499, 781
MaeI CTAG 3 cut(s) 261, 329, 938
MaeII ACGT 5 cut(s) 180, 355, 645, 718, 1068
MaeIII GTNAC 3 cut(s) 79, 874, 996
MalI GATC 4 cut(s) 572, 882, 1028, 1117
MboI GATC 4 cut(s) 570, 880, 1026, 1115
MboII GAAGA 6 cut(s) 284, 362, 485, 806, 956, 1043
MflI RGATCY 1 cut(s) 1115
MhlI GDGCHC 1 cut(s) 627
MluCI AATT 8 cut(s) 57, 150, 421, 535, 613, 687, 909, 1048
MlyI GAGTC 1 cut(s) 514
MmeI TCCRAC 1 cut(s) 763
MnlI CCTC 8 cut(s) 150, 174, 279, 387, 448, 568, 781, 794
MroNI GCCGGC 1 cut(s) 237
MroXI GAANNNNTTC 2 cut(s) 57, 192
MseI TTAA 1 cut(s) 255
MspI CCGG 3 cut(s) 238, 426, 983
Mva1269I GAATGC 1 cut(s) 159
MvnI CGCG 1 cut(s) 1025
MwoI GCNNNNNNNGC 5 cut(s) 92, 240, 247, 311, 371
NaeI GCCGGC 1 cut(s) 239
NdeI CATATG 2 cut(s) 280, 724
NdeII GATC 4 cut(s) 570, 880, 1026, 1115
NgoMIV GCCGGC 1 cut(s) 237
NlaIII CATG 5 cut(s) 436, 505, 571, 901, 1096
NmuCI GTSAC 2 cut(s) 79, 874
NspI RCATGY 1 cut(s) 505
NspV TTCGAA 1 cut(s) 483
PagI TCATGA 1 cut(s) 897
PceI AGGCCT 1 cut(s) 130
PcsI WCGNNNNNNNCGW 1 cut(s) 1035
PctI GAATGC 1 cut(s) 159
PdiI GCCGGC 1 cut(s) 239
PdmI GAANNNNTTC 2 cut(s) 57, 192
PfeI GAWTC 2 cut(s) 698, 728
PflMI CCANNNNNTGG 1 cut(s) 978
PkrI GCNGC 5 cut(s) 88, 236, 249, 514, 1098
Ple19I CGATCG 1 cut(s) 1029
PleI GAGTC 1 cut(s) 514
PmaCI CACGTG 1 cut(s) 181
PmlI CACGTG 1 cut(s) 181
PpsI GAGTC 1 cut(s) 514
Ppu21I YACGTR 3 cut(s) 181, 646, 1069
Psp124BI GAGCTC 1 cut(s) 627
PspCI CACGTG 1 cut(s) 181
PspPI GGNCC 1 cut(s) 416
PsuI RGATCY 1 cut(s) 1115
PvuI CGATCG 1 cut(s) 1029
RsaI GTAC 1 cut(s) 721
RsaNI GTAC 1 cut(s) 720
SacI GAGCTC 1 cut(s) 627
SaqAI TTAA 1 cut(s) 255
SatI GCNGC 5 cut(s) 87, 235, 248, 513, 1097
Sau3AI GATC 4 cut(s) 570, 880, 1026, 1115
Sau96I GGNCC 1 cut(s) 416
SchI GAGTC 1 cut(s) 514
SduI GDGCHC 1 cut(s) 627
SfaNI GCATC 2 cut(s) 499, 781
SfuI TTCGAA 1 cut(s) 483
SmlI CTYRAG 1 cut(s) 96
SmoI CTYRAG 1 cut(s) 96
SnaBI TACGTA 1 cut(s) 1069
Sse9I AATT 8 cut(s) 57, 150, 421, 535, 613, 687, 909, 1048
SseBI AGGCCT 1 cut(s) 130
SsiI CCGC 2 cut(s) 234, 248
SspMI CTAG 3 cut(s) 261, 329, 938
SstI GAGCTC 1 cut(s) 627
StuI AGGCCT 1 cut(s) 130
TaaI ACNGT 3 cut(s) 529, 658, 837
TaiI ACGT 5 cut(s) 183, 358, 648, 721, 1071
TaqI TCGA 6 cut(s) 67, 483, 573, 604, 1009, 1029
TasI AATT 8 cut(s) 57, 150, 421, 535, 613, 687, 909, 1048
TauI GCSGC 2 cut(s) 237, 250
TfiI GAWTC 2 cut(s) 698, 728
Tru1I TTAA 1 cut(s) 255
Tru9I TTAA 1 cut(s) 255
TseFI GTSAC 2 cut(s) 79, 874
TseI GCWGC 3 cut(s) 86, 512, 1096
Tsp45I GTSAC 2 cut(s) 79, 874
TspDTI ATGAA 4 cut(s) 66, 417, 886, 914
TspGWI ACGGA 1 cut(s) 602
Van91I CCANNNNNTGG 1 cut(s) 978
XapI RAATTY 3 cut(s) 57, 421, 613
XceI RCATGY 1 cut(s) 505
XmnI GAANNNNTTC 2 cut(s) 57, 192
XspI CTAG 3 cut(s) 261, 329, 938
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.