FvH4_4g00101

L-threonine ammonia-lyase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0019660
Physical Location & Seq
Forward (+)
122697 .. 123218
522 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g00101.t1

Sequence Viewer

Length: 522 bp
ATGGCTCCGGCAAGGACGGTACAAGTCTCTGTGCCCTCCACCACCCCTCCAAATTCCGCCTTTGTACAACCACTCCCTCAAGATGAAAACACCGGTATCTTAACTTCTTCATCTTATGAACCCGAAGAAGTAACTCAAACCCATCTTGCTGAAGTAAGGCAGGGTATGGAAATCTTGCGCGAGGAAATGCAAGAAGCTGCAGTCTCAGCCACGTCCCAGCAACATGCCTTTGAAAATCGCATCACAGCTCACCTCGCAGCCATGCAAAACACCATGGTGCAATGCCTAGTGCACCGCCAGCCCCACCACATTCAACACCACCCGGTTCCTCATCTTTCCCAGAAATCACCTTCGGCAGCCTCTCTGACGCAGGTCTCCCCCTCCCTCCCACACCATTTCCAAATCAACTCACCATTCACCAAGGTTCCCCACATACTCCAACCCTCACAGTCACACCACCCGTTGAAGCCACCATCACATCCTTCCCTCCACTTTCCCTGCCTACCTCTACTACCCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag gag-asp_proteas rve

Protein Analysis

174

Amino Acids

19.13

Weight (kDa)

6.58

Isoelectric Point (pI)

68.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019660)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06281 FvH4_4g00101
rosa_chinensis RchiOBHm_Chr1g0339791 RchiOBHm_Chr4g0426521
rosa_multiflora Rmu_sc0007466.1_g000003
rosa_samantha Rh6CG335600
rosa_wichuraiana Rw0G010860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 361
AccII CGCG 1 cut(s) 180
AciI CCGC 2 cut(s) 57, 295
AcsI RAATTY 1 cut(s) 52
AcuI CTGAAG 1 cut(s) 171
AfaI GTAC 2 cut(s) 21, 66
AgeI ACCGGT 1 cut(s) 92
AgsI TTSAA 3 cut(s) 233, 314, 466
AjiI CACGTC 1 cut(s) 213
AleI CACNNNNGTG 1 cut(s) 275
AluBI AGCT 2 cut(s) 197, 248
AluI AGCT 2 cut(s) 197, 248
Alw21I GWGCWC 1 cut(s) 294
Alw26I GTCTC 3 cut(s) 31, 208, 379
Alw44I GTGCAC 1 cut(s) 290
ApaLI GTGCAC 1 cut(s) 290
ApeKI GCWGC 3 cut(s) 197, 257, 356
ApoI RAATTY 1 cut(s) 52
AsiGI ACCGGT 1 cut(s) 92
AspLEI GCGC 1 cut(s) 180
AsuC2I CCSGG 1 cut(s) 323
AsuHPI GGTGA 4 cut(s) 242, 339, 402, 409
BaeGI GKGCMC 2 cut(s) 36, 294
BaeI ACNNNNGTAYC 2 cut(s) 79, 112
BarI GAAGNNNNNNTAC 2 cut(s) 88, 120
Bbv12I GWGCWC 1 cut(s) 294
BbvI GCAGC 3 cut(s) 184, 269, 368
BccI CCATC 2 cut(s) 150, 481
BcnI CCSGG 1 cut(s) 323
BcoDI GTCTC 3 cut(s) 31, 208, 379
BfaI CTAG 1 cut(s) 287
BfmI CTRYAG 1 cut(s) 198
BfuAI ACCTGC 1 cut(s) 361
BisI GCNGC 3 cut(s) 198, 258, 357
BlsI GCNGC 3 cut(s) 199, 259, 358
Bme1390I CCNGG 1 cut(s) 323
BmgBI CACGTC 1 cut(s) 213
BmiI GGNNCC 3 cut(s) 6, 327, 426
BmrFI CCNGG 1 cut(s) 323
BmsI GCATC 1 cut(s) 249
BoxI GACNNNNGTC 1 cut(s) 371
BpuEI CTTGAG 1 cut(s) 63
BpuMI CCSGG 1 cut(s) 323
BsaI GGTCTC 1 cut(s) 379
BsaJI CCNNGG 2 cut(s) 273, 420
BsaWI WCCGGW 1 cut(s) 92
BsaXI ACNNNNNCTCC 4 cut(s) 31, 57, 61, 87
Bse118I RCCGGY 1 cut(s) 92
Bse3DI GCAATG 1 cut(s) 287
BseDI CCNNGG 2 cut(s) 273, 420
BseGI GGATG 1 cut(s) 478
BseMI GCAATG 1 cut(s) 287
BseMII CTCAG 1 cut(s) 219
BseSI GKGCMC 2 cut(s) 36, 294
BseXI GCAGC 3 cut(s) 184, 269, 368
BseYI CCCAGC 1 cut(s) 216
Bsh1236I CGCG 1 cut(s) 180
BshTI ACCGGT 1 cut(s) 92
BsiHKAI GWGCWC 1 cut(s) 294
BsiSI CCGG 3 cut(s) 8, 93, 323
BslFI GGGAC 1 cut(s) 199
BsmAI GTCTC 3 cut(s) 31, 208, 379
BsmFI GGGAC 1 cut(s) 199
Bso31I GGTCTC 1 cut(s) 379
Bsp1286I GDGCHC 2 cut(s) 36, 294
Bsp1407I TGTACA 1 cut(s) 64
Bsp19I CCATGG 1 cut(s) 273
BspACI CCGC 2 cut(s) 57, 295
BspCNI CTCAG 1 cut(s) 218
BspFNI CGCG 1 cut(s) 180
BspLI GGNNCC 3 cut(s) 6, 327, 426
BspMAI CTGCAG 1 cut(s) 202
BspMI ACCTGC 1 cut(s) 361
BspTNI GGTCTC 1 cut(s) 379
BsrDI GCAATG 1 cut(s) 287
BsrFI RCCGGY 1 cut(s) 92
BsrGI TGTACA 1 cut(s) 64
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 2 cut(s) 273, 420
BssT1I CCWWGG 2 cut(s) 273, 420
Bst4CI ACNGT 2 cut(s) 19, 450
BstAUI TGTACA 1 cut(s) 64
BstC8I GCNNGC 1 cut(s) 299
BstDEI CTNAG 1 cut(s) 205
BstDSI CCRYGG 1 cut(s) 273
BstF5I GGATG 1 cut(s) 478
BstFNI CGCG 1 cut(s) 180
BstHHI GCGC 1 cut(s) 180
BstMAI GTCTC 3 cut(s) 31, 208, 379
BstMWI GCNNNNNNNGC 3 cut(s) 206, 254, 298
BstNSI RCATGY 1 cut(s) 227
BstPAI GACNNNNGTC 1 cut(s) 371
BstSCI CCNGG 1 cut(s) 321
BstSFI CTRYAG 1 cut(s) 198
BstSLI GKGCMC 2 cut(s) 36, 294
BstUI CGCG 1 cut(s) 180
BstV1I GCAGC 3 cut(s) 184, 269, 368
BtgI CCRYGG 1 cut(s) 273
BtrI CACGTC 1 cut(s) 213
BtsCI GGATG 1 cut(s) 478
BveI ACCTGC 1 cut(s) 361
Cac8I GCNNGC 1 cut(s) 299
CfoI GCGC 1 cut(s) 180
Cfr10I RCCGGY 1 cut(s) 92
CseI GACGC 1 cut(s) 376
Csp6I GTAC 2 cut(s) 20, 65
CspAI ACCGGT 1 cut(s) 92
CviAII CATG 4 cut(s) 224, 262, 274, 517
CviJI RGCY 8 cut(s) 5, 197, 209, 248, 260, 301, 359, 469
CviKI_1 RGCY 8 cut(s) 5, 197, 209, 248, 260, 301, 359, 469
CviQI GTAC 2 cut(s) 20, 65
DdeI CTNAG 1 cut(s) 205
EciI GGCGGA 1 cut(s) 46
Eco130I CCWWGG 2 cut(s) 273, 420
Eco31I GGTCTC 1 cut(s) 379
Eco57I CTGAAG 1 cut(s) 171
EcoT14I CCWWGG 2 cut(s) 273, 420
ErhI CCWWGG 2 cut(s) 273, 420
FaeI CATG 4 cut(s) 227, 265, 277, 520
FaiI YATR 7 cut(s) 117, 167, 225, 263, 275, 434, 518
FaqI GGGAC 1 cut(s) 199
FatI CATG 4 cut(s) 223, 261, 273, 516
Fnu4HI GCNGC 3 cut(s) 198, 258, 357
FokI GGATG 1 cut(s) 465
Fsp4HI GCNGC 3 cut(s) 198, 258, 357
FspBI CTAG 1 cut(s) 287
GlaI GCGC 1 cut(s) 179
GluI GCNGC 3 cut(s) 198, 258, 357
GsaI CCCAGC 1 cut(s) 220
HapII CCGG 3 cut(s) 8, 93, 323
HgaI GACGC 1 cut(s) 376
HhaI GCGC 1 cut(s) 180
Hin1II CATG 4 cut(s) 227, 265, 277, 520
Hin6I GCGC 1 cut(s) 178
HinP1I GCGC 1 cut(s) 178
HpaII CCGG 3 cut(s) 8, 93, 323
HphI GGTGA 4 cut(s) 242, 339, 402, 409
Hpy166II GTNNAC 1 cut(s) 292
Hpy188I TCNGA 1 cut(s) 366
Hpy188III TCNNGA 1 cut(s) 80
Hpy8I GTNNAC 1 cut(s) 292
HpyAV CCTTC 2 cut(s) 360, 492
HpyCH4III ACNGT 2 cut(s) 19, 450
HpyCH4IV ACGT 1 cut(s) 212
HpyCH4V TGCA 5 cut(s) 190, 200, 265, 280, 292
HpyF10VI GCNNNNNNNGC 3 cut(s) 206, 254, 298
HpyF3I CTNAG 1 cut(s) 205
HpySE526I ACGT 1 cut(s) 212
Hsp92II CATG 4 cut(s) 227, 265, 277, 520
HspAI GCGC 1 cut(s) 178
LmnI GCTCC 1 cut(s) 10
LpnPI CCDG 9 cut(s) 21, 106, 146, 230, 311, 336, 353, 356, 511
Lsp1109I GCAGC 3 cut(s) 184, 269, 368
LweI GCATC 1 cut(s) 249
MaeI CTAG 1 cut(s) 287
MaeII ACGT 1 cut(s) 212
MaeIII GTNAC 2 cut(s) 130, 450
MboII GAAGA 2 cut(s) 99, 137
MhlI GDGCHC 2 cut(s) 36, 294
MluCI AATT 1 cut(s) 52
MmeI TCCRAC 1 cut(s) 463
MseI TTAA 1 cut(s) 101
MslI CAYNNNNRTG 1 cut(s) 275
MspI CCGG 3 cut(s) 8, 93, 323
MspR9I CCNGG 1 cut(s) 323
MvnI CGCG 1 cut(s) 180
MwoI GCNNNNNNNGC 3 cut(s) 206, 254, 298
NciI CCSGG 1 cut(s) 323
NcoI CCATGG 1 cut(s) 273
NlaIII CATG 4 cut(s) 227, 265, 277, 520
NlaIV GGNNCC 3 cut(s) 6, 327, 426
NmuCI GTSAC 1 cut(s) 450
NspI RCATGY 1 cut(s) 227
OliI CACNNNNGTG 1 cut(s) 275
PinAI ACCGGT 1 cut(s) 92
PkrI GCNGC 3 cut(s) 199, 259, 358
PshAI GACNNNNGTC 1 cut(s) 371
PspFI CCCAGC 1 cut(s) 216
PspN4I GGNNCC 3 cut(s) 6, 327, 426
PstI CTGCAG 1 cut(s) 202
RsaI GTAC 2 cut(s) 21, 66
RsaNI GTAC 2 cut(s) 20, 65
RseI CAYNNNNRTG 1 cut(s) 275
SaqAI TTAA 1 cut(s) 101
SatI GCNGC 3 cut(s) 198, 258, 357
ScrFI CCNGG 1 cut(s) 323
SduI GDGCHC 2 cut(s) 36, 294
SetI ASST 8 cut(s) 199, 215, 250, 255, 352, 375, 426, 508
SfaNI GCATC 1 cut(s) 249
SfcI CTRYAG 1 cut(s) 198
SmiMI CAYNNNNRTG 1 cut(s) 275
SmlI CTYRAG 1 cut(s) 78
SmoI CTYRAG 1 cut(s) 78
Sse9I AATT 1 cut(s) 52
SsiI CCGC 2 cut(s) 57, 295
SspMI CTAG 1 cut(s) 287
StyD4I CCNGG 1 cut(s) 321
StyI CCWWGG 2 cut(s) 273, 420
TaaI ACNGT 2 cut(s) 19, 450
TaiI ACGT 1 cut(s) 215
TasI AATT 1 cut(s) 52
TatI WGTACW 1 cut(s) 64
Tru1I TTAA 1 cut(s) 101
Tru9I TTAA 1 cut(s) 101
TseFI GTSAC 1 cut(s) 450
TseI GCWGC 3 cut(s) 197, 257, 356
Tsp45I GTSAC 1 cut(s) 450
TspDTI ATGAA 3 cut(s) 99, 99, 132
VneI GTGCAC 1 cut(s) 290
XapI RAATTY 1 cut(s) 52
XceI RCATGY 1 cut(s) 227
XspI CTAG 1 cut(s) 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.