RchiOBHm_Chr4g0426521

L-threonine ammonia-lyase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0019660
Physical Location & Seq
Reverse (-)
52010395 .. 52012005
1611 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39556

Sequence Viewer

Length: 1449 bp
ATGGCTCGGACCGTGCAAGCTCCTGCTCCCTCCTCCACCCCAGTCACCTCCGCTTTGGTTCCACCACCACCCCCTCCTCCAATTACCCACCTAGCTTCTTCCTCTTATCAACCAACTGATGCAGCAGAACCTTTACAAAGGGCAGAATTTCACAACTCCTTAGCCATTCTTCAAGAGGAATTCCACCAGGCAATTGACGCAGTCAACAATAACCATACATCCTTTCAAAATCACATCACTGCACGACTTGCAGCTTTGCAAAGCACTATGATGCAAGTTCTTACTGGACAACACCAACCCCTTAGCTCGGCATCTCCCTCCACCCCCACTTCAACTCCATCATTTTCACCACTTCCACCACCGCGTACACCGGCCCACATCCCACCTATAACATTTGGGTCCATCTCAACCCCAATCACTTCTTACCAAGGTTTGAATTCTCCTACTCCTTTCCCAGCTCTCACATCACCAATACCTACACGTCCCGAGTCCTATCCACACACACATCAACCATTGCCATCTGCACCACCGCAATATTCCACCATTTCATCAATTCCACAACCACAATTTACCACCACAAATAACGCTTACCACACCCAAGCTCACCACACACTTCAGTATGAACCCCAATTCTTCAGACCCCCAAAAGTCGACTTACCTCGATTTACGGGTGATGATGCTGCCGGTTGGATCACCATGGTCGAAAGGTATTTGCGCACCCAGCGCATACCACAATCAGAAAAGGTGGCCATTGCAGCTTCCCATTTTGGACCCGATGCTTGTTTTTGGATGAACTCGTTTGAACAACGACATCCATTGATTACTTGGGAGCAGTTTGTACCGGCTTTCTTGGCCCATTTCGGCGCAGGTGCGAATCATGATTTCAAGATTGCACTATCACACCTGCAGCAGACGTCAACAGTGGAGCTCTTCATCACCGCCTTCACCAAACTGTCCTGCAGAGCCCCGGATTGGAACGATGATCATCTTCTTCCCATGTTTTTGGGCGGCCTGAAACCGGCAATCCGCAACGATGTCAAAACCTTCAATCCTCTCACTTTGACAGAGGCACAAAGGCTGGCACGATGCTGTGAGGCTAAGCTTTCCGAATTCCAGTCGACCTCCGACCTTCCCCCCAATCCTCCATACTCAACCACTTCCCCATTACCTTACCGTGCCAACCATACCTTGTCCTCACCTGCTACCACCCAACCACCTAACCCACCACAAGTACAACTTACCAAAACCCCGACCCATCGCAATCCCAGAATCCTTTCCACATCAGAACAACGTGAGAGAAGAGCAAAAGGTTTATGCTTTAATTGTGATGAGCAGTACACCCCCACCCACTCATGTAAACAACCATTCCTAGCCATACTGGAAGCACCTACTGCCACACCAAGTGACGAATCCTCTGACACTCTAGATTTTCCTGCATTTGGTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag gag-asp_proteas rve

Protein Analysis

482

Amino Acids

53.05

Weight (kDa)

6.59

Isoelectric Point (pI)

55.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ty3_capsid PF19259 239 - 363 2.9e-13 Ty3 transposon capsid-like protein
Retrotrans_gag PF03732 251 - 340 3.8e-12 Retrotransposon gag protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0019660)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06281 FvH4_4g00101
rosa_chinensis RchiOBHm_Chr1g0339791 RchiOBHm_Chr4g0426521
rosa_multiflora Rmu_sc0007466.1_g000003
rosa_samantha Rh6CG335600
rosa_wichuraiana Rw0G010860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 857, 912, 1207
AatII GACGTC 1 cut(s) 917
Acc16I TGCGCA 1 cut(s) 716
Acc36I ACCTGC 3 cut(s) 857, 912, 1207
AccB1I GGYRCC 1 cut(s) 1442
AccI GTMKAC 2 cut(s) 651, 1118
AccII CGCG 1 cut(s) 364
AciI CCGC 6 cut(s) 51, 362, 530, 939, 1008, 1027
AclWI GGATC 1 cut(s) 698
AcoI YGGCCR 1 cut(s) 747
AcsI RAATTY 4 cut(s) 146, 179, 436, 1109
AcuI CTGAAG 2 cut(s) 599, 619
AcyI GRCGYC 1 cut(s) 914
AdeI CACNNNGTG 1 cut(s) 1403
AfaI GTAC 4 cut(s) 367, 840, 1233, 1337
AfiI CCNNNNNNNGG 4 cut(s) 307, 972, 1018, 1439
AflIII ACRYGT 1 cut(s) 479
AgsI TTSAA 7 cut(s) 173, 227, 333, 436, 803, 886, 1048
AjiI CACGTC 1 cut(s) 482
AjnI CCWGG 1 cut(s) 186
AluBI AGCT 9 cut(s) 20, 95, 254, 306, 458, 602, 758, 928, 1102
AluI AGCT 9 cut(s) 20, 95, 254, 306, 458, 602, 758, 928, 1102
Alw21I GWGCWC 1 cut(s) 930
AlwI GGATC 1 cut(s) 698
Ama87I CYCGRG 1 cut(s) 485
AoxI GGCC 4 cut(s) 372, 747, 852, 1009
ApeKI GCWGC 5 cut(s) 122, 251, 680, 755, 907
ApoI RAATTY 4 cut(s) 146, 179, 436, 1109
Asp700I GAANNNNTTC 1 cut(s) 1273
AspLEI GCGC 3 cut(s) 717, 726, 866
AspS9I GGNCC 5 cut(s) 9, 373, 399, 770, 853
AsuC2I CCSGG 1 cut(s) 968
AsuHPI GGTGA 9 cut(s) 37, 339, 459, 596, 683, 685, 928, 937, 1188
AvaI CYCGRG 1 cut(s) 485
AvaII GGWCC 3 cut(s) 9, 399, 770
BalI TGGCCA 1 cut(s) 749
BanI GGYRCC 1 cut(s) 1442
BanII GRGCYC 2 cut(s) 930, 967
Bbv12I GWGCWC 1 cut(s) 930
BbvI GCAGC 5 cut(s) 134, 263, 667, 767, 919
BccI CCATC 4 cut(s) 346, 410, 526, 1263
BciT130I CCWGG 1 cut(s) 188
BclI TGATCA 1 cut(s) 982
BcnI CCSGG 1 cut(s) 968
BfaI CTAG 3 cut(s) 92, 1370, 1424
BfmI CTRYAG 2 cut(s) 905, 958
BfuAI ACCTGC 3 cut(s) 857, 912, 1207
BisI GCNGC 6 cut(s) 123, 252, 681, 756, 908, 1009
BlpI GCTNAGC 1 cut(s) 1098
BlsI GCNGC 6 cut(s) 124, 253, 682, 757, 909, 1010
Bme1390I CCNGG 2 cut(s) 188, 968
Bme18I GGWCC 3 cut(s) 9, 399, 770
BmeT110I CYCGRG 1 cut(s) 485
BmgBI CACGTC 1 cut(s) 482
BmgT120I GGNCC 5 cut(s) 9, 373, 399, 770, 853
BmiI GGNNCC 4 cut(s) 60, 400, 772, 1444
BmrFI CCNGG 2 cut(s) 188, 968
BmrI ACTGGG 1 cut(s) 35
BmsI GCATC 6 cut(s) 109, 261, 320, 667, 766, 1076
BmuI ACTGGG 1 cut(s) 35
Bpu10I CCTNAGC 2 cut(s) 160, 302
Bpu1102I GCTNAGC 1 cut(s) 1098
BpuMI CCSGG 1 cut(s) 968
BsaBI GATNNNNATC 1 cut(s) 984
BsaHI GRCGYC 1 cut(s) 914
BsaJI CCNNGG 3 cut(s) 427, 696, 966
BsaXI ACNNNNNCTCC 2 cut(s) 319, 349
Bsc4I CCNNNNNNNGG 4 cut(s) 307, 972, 1018, 1439
Bse118I RCCGGY 4 cut(s) 370, 683, 841, 1018
Bse1I ACTGG 4 cut(s) 41, 289, 1114, 1383
Bse3DI GCAATG 2 cut(s) 512, 750
Bse8I GATNNNNATC 1 cut(s) 984
BseBI CCWGG 1 cut(s) 188
BseDI CCNNGG 3 cut(s) 427, 696, 966
BseGI GGATG 4 cut(s) 218, 378, 795, 811
BseJI GATNNNNATC 1 cut(s) 984
BseLI CCNNNNNNNGG 4 cut(s) 307, 972, 1018, 1439
BseMI GCAATG 2 cut(s) 512, 750
BseNI ACTGG 4 cut(s) 41, 289, 1114, 1383
BseRI GAGGAG 2 cut(s) 22, 66
BseXI GCAGC 5 cut(s) 134, 263, 667, 767, 919
BseYI CCCAGC 2 cut(s) 454, 720
BsgI GTGCAG 2 cut(s) 225, 507
Bsh1236I CGCG 1 cut(s) 364
BshFI GGCC 4 cut(s) 374, 749, 854, 1011
BshNI GGYRCC 1 cut(s) 1442
BsiHKAI GWGCWC 1 cut(s) 930
BsiHKCI CYCGRG 1 cut(s) 485
BsiSI CCGG 5 cut(s) 371, 684, 842, 968, 1019
BslFI GGGAC 1 cut(s) 468
BslI CCNNNNNNNGG 4 cut(s) 307, 972, 1018, 1439
BsmFI GGGAC 1 cut(s) 468
BsnI GGCC 4 cut(s) 374, 749, 854, 1011
BsoBI CYCGRG 1 cut(s) 485
Bsp1286I GDGCHC 2 cut(s) 930, 967
Bsp143I GATC 2 cut(s) 690, 982
Bsp1720I GCTNAGC 1 cut(s) 1098
Bsp19I CCATGG 1 cut(s) 696
BspACI CCGC 6 cut(s) 51, 362, 530, 939, 1008, 1027
BspANI GGCC 4 cut(s) 374, 749, 854, 1011
BspFNI CGCG 1 cut(s) 364
BspHI TCATGA 1 cut(s) 877
BspLI GGNNCC 4 cut(s) 60, 400, 772, 1444
BspMAI CTGCAG 2 cut(s) 909, 962
BspMI ACCTGC 3 cut(s) 857, 912, 1207
BspPI GGATC 1 cut(s) 698
BspQI GCTCTTC 2 cut(s) 935, 1294
BspT107I GGYRCC 1 cut(s) 1442
BsrDI GCAATG 2 cut(s) 512, 750
BsrFI RCCGGY 4 cut(s) 370, 683, 841, 1018
BsrI ACTGG 4 cut(s) 41, 289, 1114, 1383
BssAI RCCGGY 4 cut(s) 370, 683, 841, 1018
BssECI CCNNGG 3 cut(s) 427, 696, 966
BssMI GATC 2 cut(s) 690, 982
BssNI GRCGYC 1 cut(s) 914
BssT1I CCWWGG 2 cut(s) 427, 696
Bst2UI CCWGG 1 cut(s) 188
Bst4CI ACNGT 4 cut(s) 13, 922, 954, 1175
Bst6I CTCTTC 2 cut(s) 935, 1294
BstACI GRCGYC 1 cut(s) 914
BstAPI GCANNNNNTGC 3 cut(s) 248, 1391, 1442
BstC8I GCNNGC 2 cut(s) 18, 1080
BstDEI CTNAG 3 cut(s) 160, 302, 1098
BstDSI CCRYGG 1 cut(s) 696
BstF5I GGATG 4 cut(s) 218, 378, 795, 811
BstFNI CGCG 1 cut(s) 364
BstHHI GCGC 3 cut(s) 717, 726, 866
BstKTI GATC 2 cut(s) 693, 985
BstMBI GATC 2 cut(s) 690, 982
BstMWI GCNNNNNNNGC 8 cut(s) 197, 248, 721, 723, 755, 851, 1391, 1442
BstNI CCWGG 1 cut(s) 188
BstSCI CCNGG 2 cut(s) 186, 966
BstSFI CTRYAG 2 cut(s) 905, 958
BstUI CGCG 1 cut(s) 364
BstV1I GCAGC 5 cut(s) 134, 263, 667, 767, 919
BstXI CCANNNNNNTGG 1 cut(s) 1003
BsuRI GGCC 4 cut(s) 374, 749, 854, 1011
BtgI CCRYGG 1 cut(s) 696
BtgZI GCGATG 1 cut(s) 1241
BtrI CACGTC 1 cut(s) 482
BtsCI GGATG 4 cut(s) 218, 378, 795, 811
BtsI GCAGTG 1 cut(s) 237
BtsIMutI CAGTG 2 cut(s) 237, 927
BveI ACCTGC 3 cut(s) 857, 912, 1207
Cac8I GCNNGC 2 cut(s) 18, 1080
CciI TCATGA 1 cut(s) 877
CfoI GCGC 3 cut(s) 717, 726, 866
Cfr10I RCCGGY 4 cut(s) 370, 683, 841, 1018
Cfr13I GGNCC 5 cut(s) 9, 373, 399, 770, 853
CpoI CGGWCCG 1 cut(s) 9
CseI GACGC 1 cut(s) 206
Csp6I GTAC 4 cut(s) 366, 839, 1232, 1336
CspI CGGWCCG 1 cut(s) 9
CviAII CATG 4 cut(s) 697, 878, 997, 1353
CviQI GTAC 4 cut(s) 366, 839, 1232, 1336
DdeI CTNAG 3 cut(s) 160, 302, 1098
DpnI GATC 2 cut(s) 692, 984
DpnII GATC 2 cut(s) 690, 982
DraIII CACNNNGTG 1 cut(s) 1403
EaeI YGGCCR 1 cut(s) 747
Eam1104I CTCTTC 2 cut(s) 935, 1294
EarI CTCTTC 2 cut(s) 935, 1294
Ecl136II GAGCTC 1 cut(s) 928
Eco130I CCWWGG 2 cut(s) 427, 696
Eco24I GRGCYC 2 cut(s) 930, 967
Eco47I GGWCC 3 cut(s) 9, 399, 770
Eco53kI GAGCTC 1 cut(s) 928
Eco57I CTGAAG 2 cut(s) 599, 619
Eco88I CYCGRG 1 cut(s) 485
EcoICRI GAGCTC 1 cut(s) 928
EcoRI GAATTC 3 cut(s) 179, 436, 1109
EcoRII CCWGG 1 cut(s) 186
EcoT14I CCWWGG 2 cut(s) 427, 696
EcoT38I GRGCYC 2 cut(s) 930, 967
ErhI CCWWGG 2 cut(s) 427, 696
FaeI CATG 4 cut(s) 700, 881, 1000, 1356
FalI AAGNNNNNCTT 2 cut(s) 1221, 1253
FaqI GGGAC 1 cut(s) 468
FatI CATG 4 cut(s) 696, 877, 996, 1352
FbaI TGATCA 1 cut(s) 982
FblI GTMKAC 2 cut(s) 651, 1118
Fnu4HI GCNGC 6 cut(s) 123, 252, 681, 756, 908, 1009
FokI GGATG 4 cut(s) 205, 365, 798, 802
FriOI GRGCYC 2 cut(s) 930, 967
Fsp4HI GCNGC 6 cut(s) 123, 252, 681, 756, 908, 1009
FspBI CTAG 3 cut(s) 92, 1370, 1424
FspI TGCGCA 1 cut(s) 716
GlaI GCGC 3 cut(s) 716, 725, 865
GluI GCNGC 6 cut(s) 123, 252, 681, 756, 908, 1009
GsaI CCCAGC 2 cut(s) 458, 724
HaeIII GGCC 4 cut(s) 374, 749, 854, 1011
HapII CCGG 5 cut(s) 371, 684, 842, 968, 1019
HgaI GACGC 1 cut(s) 206
HhaI GCGC 3 cut(s) 717, 726, 866
Hin1I GRCGYC 1 cut(s) 914
Hin1II CATG 4 cut(s) 700, 881, 1000, 1356
Hin6I GCGC 3 cut(s) 715, 724, 864
HinP1I GCGC 3 cut(s) 715, 724, 864
HincII GTYRAC 4 cut(s) 205, 652, 918, 1119
HindII GTYRAC 4 cut(s) 205, 652, 918, 1119
HindIII AAGCTT 1 cut(s) 1100
HinfI GANTC 4 cut(s) 488, 874, 1269, 1409
HpaII CCGG 5 cut(s) 371, 684, 842, 968, 1019
HphI GGTGA 9 cut(s) 37, 339, 459, 596, 683, 685, 928, 937, 1188
Hpy166II GTNNAC 7 cut(s) 205, 368, 652, 918, 1119, 1338, 1358
Hpy188I TCNGA 7 cut(s) 9, 638, 739, 1108, 1126, 1285, 1417
Hpy188III TCNNGA 5 cut(s) 173, 485, 878, 886, 1424
Hpy8I GTNNAC 7 cut(s) 205, 368, 652, 918, 1119, 1338, 1358
HpyAV CCTTC 3 cut(s) 952, 1054, 1139
HpyCH4III ACNGT 4 cut(s) 13, 922, 954, 1175
HpyCH4IV ACGT 3 cut(s) 481, 914, 1291
HpyF10VI GCNNNNNNNGC 8 cut(s) 197, 248, 721, 723, 755, 851, 1391, 1442
HpyF3I CTNAG 3 cut(s) 160, 302, 1098
HpySE526I ACGT 3 cut(s) 481, 914, 1291
Hsp92I GRCGYC 1 cut(s) 914
Hsp92II CATG 4 cut(s) 700, 881, 1000, 1356
HspAI GCGC 3 cut(s) 715, 724, 864
Ksp22I TGATCA 1 cut(s) 982
Kzo9I GATC 2 cut(s) 690, 982
LguI GCTCTTC 2 cut(s) 935, 1294
LmnI GCTCC 4 cut(s) 25, 31, 829, 925
Lsp1109I GCAGC 5 cut(s) 134, 263, 667, 767, 919
LweI GCATC 6 cut(s) 109, 261, 320, 667, 766, 1076
MaeI CTAG 3 cut(s) 92, 1370, 1424
MaeII ACGT 3 cut(s) 481, 914, 1291
MaeIII GTNAC 2 cut(s) 43, 1403
MalI GATC 2 cut(s) 692, 984
MboI GATC 2 cut(s) 690, 982
MboII GAAGA 7 cut(s) 90, 161, 625, 922, 980, 983, 1311
MfeI CAATTG 1 cut(s) 192
MhlI GDGCHC 2 cut(s) 930, 967
MlsI TGGCCA 1 cut(s) 749
MluNI TGGCCA 1 cut(s) 749
MlyI GAGTC 1 cut(s) 497
MmeI TCCRAC 2 cut(s) 668, 1149
Mox20I TGGCCA 1 cut(s) 749
MroXI GAANNNNTTC 1 cut(s) 1273
MscI TGGCCA 1 cut(s) 749
MseI TTAA 1 cut(s) 1320
MslI CAYNNNNRTG 1 cut(s) 269
Msp20I TGGCCA 1 cut(s) 749
MspI CCGG 5 cut(s) 371, 684, 842, 968, 1019
MspR9I CCNGG 2 cut(s) 188, 968
MunI CAATTG 1 cut(s) 192
MvaI CCWGG 1 cut(s) 188
MvnI CGCG 1 cut(s) 364
MwoI GCNNNNNNNGC 8 cut(s) 197, 248, 721, 723, 755, 851, 1391, 1442
NciI CCSGG 1 cut(s) 968
NcoI CCATGG 1 cut(s) 696
NdeII GATC 2 cut(s) 690, 982
NlaIII CATG 4 cut(s) 700, 881, 1000, 1356
NlaIV GGNNCC 4 cut(s) 60, 400, 772, 1444
NmeAIII GCCGAG 1 cut(s) 287
NmuCI GTSAC 2 cut(s) 43, 1403
NsbI TGCGCA 1 cut(s) 716
PagI TCATGA 1 cut(s) 877
PaqCI CACCTGC 3 cut(s) 857, 912, 1207
PciSI GCTCTTC 2 cut(s) 935, 1294
PdmI GAANNNNTTC 1 cut(s) 1273
PfeI GAWTC 3 cut(s) 874, 1269, 1409
PflFI GACNNNGTC 1 cut(s) 200
PkrI GCNGC 6 cut(s) 124, 253, 682, 757, 909, 1010
PleI GAGTC 1 cut(s) 496
PpsI GAGTC 1 cut(s) 496
Psp124BI GAGCTC 1 cut(s) 930
Psp6I CCWGG 1 cut(s) 186
PspFI CCCAGC 2 cut(s) 454, 720
PspGI CCWGG 1 cut(s) 186
PspN4I GGNNCC 4 cut(s) 60, 400, 772, 1444
PspPI GGNCC 5 cut(s) 9, 373, 399, 770, 853
PstI CTGCAG 2 cut(s) 909, 962
PsyI GACNNNGTC 1 cut(s) 200
RsaI GTAC 4 cut(s) 367, 840, 1233, 1337
RsaNI GTAC 4 cut(s) 366, 839, 1232, 1336
RseI CAYNNNNRTG 1 cut(s) 269
Rsr2I CGGWCCG 1 cut(s) 9
RsrII CGGWCCG 1 cut(s) 9
SacI GAGCTC 1 cut(s) 930
SalI GTCGAC 2 cut(s) 650, 1117
SapI GCTCTTC 2 cut(s) 935, 1294
SaqAI TTAA 1 cut(s) 1320
SatI GCNGC 6 cut(s) 123, 252, 681, 756, 908, 1009
Sau3AI GATC 2 cut(s) 690, 982
Sau96I GGNCC 5 cut(s) 9, 373, 399, 770, 853
SchI GAGTC 1 cut(s) 497
ScrFI CCNGG 2 cut(s) 188, 968
SduI GDGCHC 2 cut(s) 930, 967
SfaNI GCATC 6 cut(s) 109, 261, 320, 667, 766, 1076
SfcI CTRYAG 2 cut(s) 905, 958
SinI GGWCC 3 cut(s) 9, 399, 770
SmiMI CAYNNNNRTG 1 cut(s) 269
SsiI CCGC 6 cut(s) 51, 362, 530, 939, 1008, 1027
SspI AATATT 1 cut(s) 536
SspMI CTAG 3 cut(s) 92, 1370, 1424
SstI GAGCTC 1 cut(s) 930
StyD4I CCNGG 2 cut(s) 186, 966
StyI CCWWGG 2 cut(s) 427, 696
TaaI ACNGT 4 cut(s) 13, 922, 954, 1175
TaiI ACGT 3 cut(s) 484, 917, 1294
TaqI TCGA 4 cut(s) 651, 661, 702, 1118
TatI WGTACW 2 cut(s) 1231, 1335
TauI GCSGC 1 cut(s) 1011
TfiI GAWTC 3 cut(s) 874, 1269, 1409
Tru1I TTAA 1 cut(s) 1320
Tru9I TTAA 1 cut(s) 1320
TscAI CASTG 2 cut(s) 244, 927
TseFI GTSAC 2 cut(s) 43, 1403
TseI GCWGC 5 cut(s) 122, 251, 680, 755, 907
Tsp45I GTSAC 2 cut(s) 43, 1403
TspDTI ATGAA 4 cut(s) 537, 636, 806, 922
TspRI CASTG 2 cut(s) 244, 927
Tth111I GACNNNGTC 1 cut(s) 200
VpaK11BI GGWCC 3 cut(s) 9, 399, 770
XapI RAATTY 4 cut(s) 146, 179, 436, 1109
XbaI TCTAGA 1 cut(s) 1423
XcmI CCANNNNNNNNNTGG 1 cut(s) 822
XmiI GTMKAC 2 cut(s) 651, 1118
XmnI GAANNNNTTC 1 cut(s) 1273
XspI CTAG 3 cut(s) 92, 1370, 1424
ZraI GACGTC 1 cut(s) 915
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.