FvH4_4g01090
WRKY Family

protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0018019
Physical Location & Seq
Forward (+)
1028897 .. 1030786
1890 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g01090.t1

Sequence Viewer

Length: 1479 bp
ATGCCGGCGCGAATGACGGAGGAAGCCACTGGAGCTTGCTGGTTTACTACAGAAAATCAAACACGTTCGTGCACTGACAGCTTGGGAACGAATGGTTCTTGTGCTAAAAAGCTTTTTCGGGCAGTGAAGAAACATGTGGCAACACCTACTGATGAGAGTAGCAGCAACGCTGCTACAATTGTTGATGGAAATGTGGATTCTACTGATGCTAATCCAATCAATATTGGAGACGCAGATTCTAACAATGCTACTACAATTGGTACTGTAGACGTGGACTCTAACCTGACTAGTACAGTCGATATTGTACATGTGGATGCTACTGCTACTAGTCCAGTGCCTCCCGTCAATGGAGATGGAGATGGAGATGGAGATTCTACCATGATCGATGGAGATTCTACTAATACTACTAGTATGACGGATGCGGATGTTTCTATTAGTAGCAACAGAGTTGGTGAAGATAATACTGGTTGTGGTAGAGTTGATAATGATGAAAACCAGCTGCGGCAACATAATCAGAAGAAAAAGAGGATGCATGGTTATCGTTACGCACGACCGTGGATTTATGGGGAGGAGGAAGAGGAGATGCCCTGGCATATGTATGGCCCTCCTTGTTCTCAACGTGATTTTCATAATTCCTTTCGGTATGGAAGGGACTCAAGGCAGGATGAGTATATGAACTCGAACTGTGCTTATGGTTATTATCGTCGACCTTATTCTAATTCGAGATGGGATATGGACAGGGATGCTCATTGGAATACTTTTCCAAGAGGTCACCGTAAAATTATCCATGAGGAAGATGATAGTTTTAAAGAAGAGGGATGCATGCCGAAGCAGAAAAATTTCTATGACTGCGGTTTGTATGTGATGGAAATTGCTAGGGTGGTGTGTGAATGGTACTACCTTGATGATGAAGCAAAGCAGCAACAGCCGGAGCATCAAGGTTTCTTGTGTAAGTTGAAGGGTGGCGGCCGGTTTCCCAACATCGGTGAGTATGTTCATACTAATTCTTTGGAATCCACAATGCGTCGCATGCTTTTGGAAATCATTCAAGAACGAGCATGCGAGTCTGGTATAACTATTGATTTATTTTCCTCTGATGATGATGATGAGTACAAGACTGAGAAAGCTATTGTGTTGTTTGATAATGATGAGTGCAAGAATGAGAAAGCTATTATGTTGTTTTCTGATAAAAATGAGTCCATGGATGATGATAGTACTTCCCTATTCGGCCCCCAGCCTCAGCCCCAACACGATAAGTCGGAGGATGAAAAACTGGAGGATTGTGGTGGTGTTGCTGCTCTTCCCCAATCTGTATCCTCAACTTCAGAGGATGAAATACTCGAGGATTGTGTTGATGTTGCTGCTCTTCCAGAATCCATATCTTCCACTACTTCAGAGGATGAGCCAGAAGAAGATTATGATTCTATGCCAGAGGACAAGTGCTCATTGTTCAGTAAATGCAGCAATTGTAGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 WRKY

Protein Analysis

493

Amino Acids

55.26

Weight (kDa)

4.47

Isoelectric Point (pI)

58.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018019)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g01090
rosa_chinensis RchiOBHm_Chr4g0387651
rosa_laevigata RLG00000010103
rosa_multiflora Rmu_sc0003953.1_g000007
rosa_roxburghii Rroxscaffold_5G00334320
rosa_rugosa Rorug03G0312200
rosa_samantha Rh4AG017500 Rh4BG012300 Rh4CG018700 Rh4DG013300
rosa_wichuraiana Rw4G001130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 267, 706
AccII CGCG 1 cut(s) 10
AciI CCGC 4 cut(s) 422, 502, 852, 966
AcoI YGGCCR 1 cut(s) 967
AcsI RAATTY 1 cut(s) 838
AcuI CTGAAG 2 cut(s) 1308, 1377
AfaI GTAC 6 cut(s) 262, 292, 306, 896, 1112, 1216
AfiI CCNNNNNNNGG 2 cut(s) 347, 983
AflIII ACRYGT 3 cut(s) 62, 133, 307
AgsI TTSAA 2 cut(s) 958, 1049
AhlI ACTAGT 3 cut(s) 287, 326, 407
AjiI CACGTC 1 cut(s) 271
AjnI CCWGG 1 cut(s) 587
AleI CACNNNNGTG 2 cut(s) 67, 553
AluBI AGCT 6 cut(s) 35, 81, 112, 499, 1127, 1169
AluI AGCT 6 cut(s) 35, 81, 112, 499, 1127, 1169
Alw21I GWGCWC 2 cut(s) 74, 1445
Alw26I GTCTC 1 cut(s) 222
Alw44I GTGCAC 1 cut(s) 70
Ama87I CYCGRG 1 cut(s) 1340
AoxI GGCC 3 cut(s) 601, 967, 1228
ApaLI GTGCAC 1 cut(s) 70
ApeKI GCWGC 7 cut(s) 162, 170, 499, 919, 1295, 1361, 1461
ApoI RAATTY 1 cut(s) 838
Asp700I GAANNNNTTC 2 cut(s) 839, 1044
AspLEI GCGC 1 cut(s) 10
AspS9I GGNCC 2 cut(s) 602, 1229
AsuHPI GGTGA 3 cut(s) 464, 764, 998
AvaI CYCGRG 1 cut(s) 1340
BaeGI GKGCMC 1 cut(s) 74
Bbv12I GWGCWC 2 cut(s) 74, 1445
BbvCI CCTCAGC 1 cut(s) 1239
BbvI GCAGC 7 cut(s) 157, 174, 486, 931, 1282, 1348, 1473
BccI CCATC 7 cut(s) 179, 347, 353, 359, 380, 720, 859
BcgI CGANNNNNNTGC 2 cut(s) 521, 555
BciT130I CCWGG 1 cut(s) 589
BciVI GTATCC 1 cut(s) 1324
BcoDI GTCTC 1 cut(s) 222
BcuI ACTAGT 3 cut(s) 287, 326, 407
BfaI CTAG 4 cut(s) 288, 327, 408, 876
BfmI CTRYAG 2 cut(s) 48, 264
BfuI GTATCC 1 cut(s) 1324
BisI GCNGC 9 cut(s) 163, 171, 500, 503, 920, 967, 1296, 1362, 1462
BlsI GCNGC 9 cut(s) 164, 172, 501, 504, 921, 968, 1297, 1363, 1463
BmcAI AGTACT 1 cut(s) 1216
Bme1390I CCNGG 1 cut(s) 589
BmeT110I CYCGRG 1 cut(s) 1340
BmgBI CACGTC 1 cut(s) 271
BmgT120I GGNCC 2 cut(s) 602, 1229
BmiI GGNNCC 1 cut(s) 1231
BmrFI CCNGG 1 cut(s) 589
BmsI GCATC 8 cut(s) 196, 304, 409, 519, 573, 733, 809, 943
BpmI CTGGAG 2 cut(s) 51, 1295
Bpu10I CCTNAGC 1 cut(s) 1239
BpuEI CTTGAG 1 cut(s) 640
Bsa29I ATCGAT 1 cut(s) 384
BsaBI GATNNNNATC 1 cut(s) 210
BsaJI CCNNGG 3 cut(s) 554, 587, 1200
Bsc4I CCNNNNNNNGG 2 cut(s) 347, 983
Bse118I RCCGGY 2 cut(s) 4, 969
Bse1I ACTGG 4 cut(s) 34, 332, 469, 1278
Bse8I GATNNNNATC 1 cut(s) 210
BseBI CCWGG 1 cut(s) 589
BseCI ATCGAT 1 cut(s) 384
BseDI CCNNGG 3 cut(s) 554, 587, 1200
BseJI GATNNNNATC 1 cut(s) 210
BseLI CCNNNNNNNGG 2 cut(s) 347, 983
BseMII CTCAG 2 cut(s) 1110, 1253
BseNI ACTGG 4 cut(s) 34, 332, 469, 1278
BseRI GAGGAG 2 cut(s) 584, 593
BseSI GKGCMC 1 cut(s) 74
BseX3I CGGCCG 1 cut(s) 967
BseXI GCAGC 7 cut(s) 157, 174, 486, 931, 1282, 1348, 1473
BseYI CCCAGC 1 cut(s) 1233
Bsh1236I CGCG 1 cut(s) 10
Bsh1285I CGRYCG 2 cut(s) 554, 970
BshFI GGCC 3 cut(s) 603, 969, 1230
BshVI ATCGAT 1 cut(s) 384
BsiEI CGRYCG 2 cut(s) 554, 970
BsiHKAI GWGCWC 2 cut(s) 74, 1445
BsiHKCI CYCGRG 1 cut(s) 1340
BsiSI CCGG 3 cut(s) 5, 929, 970
BslFI GGGAC 1 cut(s) 665
BslI CCNNNNNNNGG 2 cut(s) 347, 983
BsmAI GTCTC 1 cut(s) 222
BsmBI CGTCTC 1 cut(s) 222
BsmFI GGGAC 1 cut(s) 665
BsnI GGCC 3 cut(s) 603, 969, 1230
BsoBI CYCGRG 1 cut(s) 1340
Bsp1286I GDGCHC 2 cut(s) 74, 1445
Bsp1407I TGTACA 1 cut(s) 304
Bsp143I GATC 1 cut(s) 381
Bsp19I CCATGG 1 cut(s) 1200
BspACI CCGC 4 cut(s) 422, 502, 852, 966
BspANI GGCC 3 cut(s) 603, 969, 1230
BspCNI CTCAG 2 cut(s) 1111, 1252
BspDI ATCGAT 1 cut(s) 384
BspFNI CGCG 1 cut(s) 10
BspLI GGNNCC 1 cut(s) 1231
BspQI GCTCTTC 2 cut(s) 1305, 1371
BsrFI RCCGGY 2 cut(s) 4, 969
BsrGI TGTACA 1 cut(s) 304
BsrI ACTGG 4 cut(s) 34, 332, 469, 1278
BssAI RCCGGY 2 cut(s) 4, 969
BssECI CCNNGG 3 cut(s) 554, 587, 1200
BssMI GATC 1 cut(s) 381
BssT1I CCWWGG 1 cut(s) 1200
Bst2UI CCWGG 1 cut(s) 589
Bst4CI ACNGT 5 cut(s) 265, 295, 555, 686, 776
Bst6I CTCTTC 4 cut(s) 570, 807, 1305, 1371
BstAUI TGTACA 1 cut(s) 304
BstC8I GCNNGC 5 cut(s) 6, 37, 824, 1031, 1060
BstDEI CTNAG 2 cut(s) 1119, 1239
BstDSI CCRYGG 2 cut(s) 554, 1200
BstEII GGTNACC 1 cut(s) 770
BstFNI CGCG 1 cut(s) 10
BstHHI GCGC 1 cut(s) 10
BstKTI GATC 1 cut(s) 384
BstMAI GTCTC 1 cut(s) 222
BstMBI GATC 1 cut(s) 381
BstMCI CGRYCG 2 cut(s) 554, 970
BstMWI GCNNNNNNNGC 4 cut(s) 32, 78, 925, 1030
BstNI CCWGG 1 cut(s) 589
BstNSI RCATGY 5 cut(s) 137, 311, 826, 1033, 1062
BstPI GGTNACC 1 cut(s) 770
BstSCI CCNGG 1 cut(s) 587
BstSFI CTRYAG 2 cut(s) 48, 264
BstSLI GKGCMC 1 cut(s) 74
BstUI CGCG 1 cut(s) 10
BstV1I GCAGC 7 cut(s) 157, 174, 486, 931, 1282, 1348, 1473
BstZI CGGCCG 1 cut(s) 967
Bsu15I ATCGAT 1 cut(s) 384
BsuI GTATCC 1 cut(s) 1324
BsuRI GGCC 3 cut(s) 603, 969, 1230
BsuTUI ATCGAT 1 cut(s) 384
BtgI CCRYGG 2 cut(s) 554, 1200
BtrI CACGTC 1 cut(s) 271
BtsI GCAGTG 1 cut(s) 129
BtsIMutI CAGTG 4 cut(s) 27, 72, 129, 339
Cac8I GCNNGC 5 cut(s) 6, 37, 824, 1031, 1060
CfoI GCGC 1 cut(s) 10
Cfr10I RCCGGY 2 cut(s) 4, 969
Cfr13I GGNCC 2 cut(s) 602, 1229
ClaI ATCGAT 1 cut(s) 384
CseI GACGC 2 cut(s) 239, 1013
Csp6I GTAC 6 cut(s) 261, 291, 305, 895, 1111, 1215
CviAII CATG 9 cut(s) 134, 308, 379, 533, 788, 823, 1030, 1059, 1201
CviQI GTAC 6 cut(s) 261, 291, 305, 895, 1111, 1215
DdeI CTNAG 2 cut(s) 1119, 1239
DpnI GATC 1 cut(s) 383
DpnII GATC 1 cut(s) 381
DraI TTTAAA 1 cut(s) 808
EaeI YGGCCR 1 cut(s) 967
EagI CGGCCG 1 cut(s) 967
Eam1104I CTCTTC 4 cut(s) 570, 807, 1305, 1371
EarI CTCTTC 4 cut(s) 570, 807, 1305, 1371
EclXI CGGCCG 1 cut(s) 967
Eco130I CCWWGG 1 cut(s) 1200
Eco52I CGGCCG 1 cut(s) 967
Eco57I CTGAAG 2 cut(s) 1308, 1377
Eco88I CYCGRG 1 cut(s) 1340
Eco91I GGTNACC 1 cut(s) 770
EcoO65I GGTNACC 1 cut(s) 770
EcoRII CCWGG 1 cut(s) 587
EcoT14I CCWWGG 1 cut(s) 1200
EcoT22I ATGCAT 2 cut(s) 534, 824
ErhI CCWWGG 1 cut(s) 1200
Esp3I CGTCTC 1 cut(s) 222
FaeI CATG 9 cut(s) 137, 311, 382, 536, 791, 826, 1033, 1062, 1204
FaqI GGGAC 1 cut(s) 665
FatI CATG 9 cut(s) 133, 307, 378, 532, 787, 822, 1029, 1058, 1200
FauNDI CATATG 1 cut(s) 594
FblI GTMKAC 2 cut(s) 267, 706
Fnu4HI GCNGC 9 cut(s) 163, 171, 500, 503, 920, 967, 1296, 1362, 1462
Fsp4HI GCNGC 9 cut(s) 163, 171, 500, 503, 920, 967, 1296, 1362, 1462
FspBI CTAG 4 cut(s) 288, 327, 408, 876
GlaI GCGC 1 cut(s) 9
GluI GCNGC 9 cut(s) 163, 171, 500, 503, 920, 967, 1296, 1362, 1462
GsaI CCCAGC 1 cut(s) 1237
GsuI CTGGAG 2 cut(s) 51, 1295
HaeIII GGCC 3 cut(s) 603, 969, 1230
HapII CCGG 3 cut(s) 5, 929, 970
HgaI GACGC 2 cut(s) 239, 1013
HhaI GCGC 1 cut(s) 10
Hin1II CATG 9 cut(s) 137, 311, 382, 536, 791, 826, 1033, 1062, 1204
Hin6I GCGC 1 cut(s) 8
HinP1I GCGC 1 cut(s) 8
HincII GTYRAC 1 cut(s) 707
HindII GTYRAC 1 cut(s) 707
HindIII AAGCTT 1 cut(s) 110
HpaII CCGG 3 cut(s) 5, 929, 970
HphI GGTGA 3 cut(s) 464, 764, 998
Hpy166II GTNNAC 5 cut(s) 45, 72, 268, 274, 707
Hpy188I TCNGA 6 cut(s) 516, 1096, 1186, 1261, 1327, 1396
Hpy188III TCNNGA 3 cut(s) 723, 1049, 1370
Hpy8I GTNNAC 5 cut(s) 45, 72, 268, 274, 707
Hpy99I CGWCG 2 cut(s) 708, 1029
HpyAV CCTTC 2 cut(s) 642, 952
HpyCH4III ACNGT 5 cut(s) 265, 295, 555, 686, 776
HpyCH4IV ACGT 3 cut(s) 64, 270, 619
HpyCH4V TGCA 5 cut(s) 72, 532, 822, 1155, 1461
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 78, 925, 1030
HpyF3I CTNAG 2 cut(s) 1119, 1239
HpySE526I ACGT 3 cut(s) 64, 270, 619
Hsp92II CATG 9 cut(s) 137, 311, 382, 536, 791, 826, 1033, 1062, 1204
HspAI GCGC 1 cut(s) 8
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Kzo9I GATC 1 cut(s) 381
LguI GCTCTTC 2 cut(s) 1305, 1371
LmnI GCTCC 2 cut(s) 32, 931
Lsp1109I GCAGC 7 cut(s) 157, 174, 486, 931, 1282, 1348, 1473
LweI GCATC 8 cut(s) 196, 304, 409, 519, 573, 733, 809, 943
MaeI CTAG 4 cut(s) 288, 327, 408, 876
MaeII ACGT 3 cut(s) 64, 270, 619
MaeIII GTNAC 2 cut(s) 542, 770
MalI GATC 1 cut(s) 383
MboI GATC 1 cut(s) 381
MfeI CAATTG 3 cut(s) 177, 255, 1465
MhlI GDGCHC 2 cut(s) 74, 1445
MluCI AATT 9 cut(s) 177, 255, 631, 718, 780, 838, 870, 1003, 1465
MlyI GAGTC 4 cut(s) 269, 647, 1073, 1205
MmeI TCCRAC 1 cut(s) 1239
Mph1103I ATGCAT 2 cut(s) 534, 824
MroNI GCCGGC 1 cut(s) 4
MroXI GAANNNNTTC 2 cut(s) 839, 1044
MseI TTAA 1 cut(s) 807
MslI CAYNNNNRTG 4 cut(s) 67, 312, 553, 597
MspA1I CMGCKG 1 cut(s) 499
MspI CCGG 3 cut(s) 5, 929, 970
MspR9I CCNGG 1 cut(s) 589
MunI CAATTG 3 cut(s) 177, 255, 1465
MvaI CCWGG 1 cut(s) 589
MvnI CGCG 1 cut(s) 10
MwoI GCNNNNNNNGC 4 cut(s) 32, 78, 925, 1030
NaeI GCCGGC 1 cut(s) 6
NcoI CCATGG 1 cut(s) 1200
NdeI CATATG 1 cut(s) 594
NdeII GATC 1 cut(s) 381
NgoMIV GCCGGC 1 cut(s) 4
NlaIII CATG 9 cut(s) 137, 311, 382, 536, 791, 826, 1033, 1062, 1204
NlaIV GGNNCC 1 cut(s) 1231
NmuCI GTSAC 1 cut(s) 770
NsiI ATGCAT 2 cut(s) 534, 824
NspI RCATGY 5 cut(s) 137, 311, 826, 1033, 1062
OliI CACNNNNGTG 2 cut(s) 67, 553
PaeI GCATGC 3 cut(s) 826, 1033, 1062
PaeR7I CTCGAG 1 cut(s) 1340
PciI ACATGT 2 cut(s) 133, 307
PciSI GCTCTTC 2 cut(s) 1305, 1371
PcsI WCGNNNNNNNCGW 1 cut(s) 547
PdiI GCCGGC 1 cut(s) 6
PdmI GAANNNNTTC 2 cut(s) 839, 1044
PfeI GAWTC 7 cut(s) 197, 236, 371, 392, 1013, 1373, 1421
PkrI GCNGC 9 cut(s) 164, 172, 501, 504, 921, 968, 1297, 1363, 1463
PleI GAGTC 4 cut(s) 269, 647, 1072, 1204
PpsI GAGTC 4 cut(s) 269, 647, 1072, 1204
PscI ACATGT 2 cut(s) 133, 307
Psp6I CCWGG 1 cut(s) 587
PspEI GGTNACC 1 cut(s) 770
PspFI CCCAGC 1 cut(s) 1233
PspGI CCWGG 1 cut(s) 587
PspN4I GGNNCC 1 cut(s) 1231
PspPI GGNCC 2 cut(s) 602, 1229
PspXI VCTCGAGB 1 cut(s) 1340
PvuII CAGCTG 1 cut(s) 499
RsaI GTAC 6 cut(s) 262, 292, 306, 896, 1112, 1216
RsaNI GTAC 6 cut(s) 261, 291, 305, 895, 1111, 1215
RseI CAYNNNNRTG 4 cut(s) 67, 312, 553, 597
SalI GTCGAC 1 cut(s) 705
SapI GCTCTTC 2 cut(s) 1305, 1371
SaqAI TTAA 1 cut(s) 807
SatI GCNGC 9 cut(s) 163, 171, 500, 503, 920, 967, 1296, 1362, 1462
Sau3AI GATC 1 cut(s) 381
Sau96I GGNCC 2 cut(s) 602, 1229
ScaI AGTACT 1 cut(s) 1216
SchI GAGTC 4 cut(s) 269, 647, 1073, 1205
ScrFI CCNGG 1 cut(s) 589
SduI GDGCHC 2 cut(s) 74, 1445
SfaNI GCATC 8 cut(s) 196, 304, 409, 519, 573, 733, 809, 943
SfcI CTRYAG 2 cut(s) 48, 264
Sfr274I CTCGAG 1 cut(s) 1340
SlaI CTCGAG 1 cut(s) 1340
SmiMI CAYNNNNRTG 4 cut(s) 67, 312, 553, 597
SmlI CTYRAG 2 cut(s) 655, 1340
SmoI CTYRAG 2 cut(s) 655, 1340
SpeI ACTAGT 3 cut(s) 287, 326, 407
SphI GCATGC 3 cut(s) 826, 1033, 1062
Sse9I AATT 9 cut(s) 177, 255, 631, 718, 780, 838, 870, 1003, 1465
SsiI CCGC 4 cut(s) 422, 502, 852, 966
SspI AATATT 1 cut(s) 223
SspMI CTAG 4 cut(s) 288, 327, 408, 876
StyD4I CCNGG 1 cut(s) 587
StyI CCWWGG 1 cut(s) 1200
TaaI ACNGT 5 cut(s) 265, 295, 555, 686, 776
TaiI ACGT 3 cut(s) 67, 273, 622
TaqI TCGA 6 cut(s) 297, 384, 680, 706, 722, 1341
TasI AATT 9 cut(s) 177, 255, 631, 718, 780, 838, 870, 1003, 1465
TatI WGTACW 4 cut(s) 290, 304, 1110, 1214
TauI GCSGC 2 cut(s) 505, 969
TfiI GAWTC 7 cut(s) 197, 236, 371, 392, 1013, 1373, 1421
Tru1I TTAA 1 cut(s) 807
Tru9I TTAA 1 cut(s) 807
TscAI CASTG 4 cut(s) 34, 79, 129, 339
TseFI GTSAC 1 cut(s) 770
TseI GCWGC 7 cut(s) 162, 170, 499, 919, 1295, 1361, 1461
Tsp45I GTSAC 1 cut(s) 770
TspDTI ATGAA 7 cut(s) 504, 617, 689, 924, 986, 1281, 1347
TspGWI ACGGA 2 cut(s) 32, 431
TspRI CASTG 4 cut(s) 34, 79, 129, 339
VneI GTGCAC 1 cut(s) 70
XapI RAATTY 1 cut(s) 838
XceI RCATGY 5 cut(s) 137, 311, 826, 1033, 1062
XhoI CTCGAG 1 cut(s) 1340
XmiI GTMKAC 2 cut(s) 267, 706
XmnI GAANNNNTTC 2 cut(s) 839, 1044
XspI CTAG 4 cut(s) 288, 327, 408, 876
ZrmI AGTACT 1 cut(s) 1216
Zsp2I ATGCAT 2 cut(s) 534, 824
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.