Rorug03G0312200
WRKY Family

protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0018019
Physical Location & Seq
Reverse (-)
33418818 .. 33419597
780 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0312200.1

Sequence Viewer

Length: 780 bp
ATGGAACCACGAACTCTGCAACTAATGGAACTTCAAACCCAGCAACAAATGGAACTACAAGCCCAGCAACCCACCGAGGAGGGAGGAAGCAACCAAGCAGCCGGGAGTAGTGCCAACACGCCTATCAGGCCGACCAATAACCGGTGGACTCCCACACCAGCTCAACTAAGAATCCTCCAGGAGCTTTACTATGACAAGGTGCTTAAATACCCAACTCCAGAGCAGATTCAAGAGATCTGCCTTCATCTGCAACAATATGGGCAGATCGAGGACAAGAACGTCTTCTTTTGGTTCCAGAACCTCAAGGCTCGTGAGAGGCAGAAGTTGAAGGAATTTCGGAACGTTCGGGTTGGTGGATCTCTCGATCTCAATTTTGGATCCACTAGTTCTACTGATGATGGTAGGTCCATAGATCTAAACTTTGGTTCCACTGGTTCTACTGGTACTGATGGATCCCTTGATCTAAATTTTGGGTCTTGTGTCGGCTATGGTGTTGACCCATATTCCTCCTCACCCTTCAACACTAATACTAGTACTACCTCCTATGGCACAATAGGAGGACAAACTTCCATGGAACAACAAGGAGGAGATCACCAGGAGGTCGAGACTCTTCCTCTGTTCCCCATGCACGGTGAGAACGTCCTTGGTAACCCGAAGACTACATCCGAGGAAGGTAGCGCATATGGTTACTATTCCGGTGGCTCAGGCGGTTACAACAGTGGCTCTCCCGTTTCTCTTGAGCTCAGCCTCAACCCAACCGGAGCTGCCGACTTGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 WRKY

Protein Analysis

259

Amino Acids

28.19

Weight (kDa)

4.59

Isoelectric Point (pI)

44.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 47 - 107 1.5e-11 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018019)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g01090
rosa_chinensis RchiOBHm_Chr4g0387651
rosa_laevigata RLG00000010103
rosa_multiflora Rmu_sc0003953.1_g000007
rosa_roxburghii Rroxscaffold_5G00334320
rosa_rugosa Rorug03G0312200
rosa_samantha Rh4AG017500 Rh4BG012300 Rh4CG018700 Rh4DG013300
rosa_wichuraiana Rw4G001130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 278
AciI CCGC 1 cut(s) 708
AclI AACGTT 1 cut(s) 342
AclWI GGATC 5 cut(s) 364, 372, 385, 447, 460
AcsI RAATTY 2 cut(s) 332, 466
AfaI GTAC 2 cut(s) 445, 535
AfiI CCNNNNNNNGG 2 cut(s) 141, 629
AgeI ACCGGT 1 cut(s) 141
AgsI TTSAA 4 cut(s) 35, 230, 328, 520
AhlI ACTAGT 2 cut(s) 383, 530
AjnI CCWGG 2 cut(s) 177, 594
AluBI AGCT 4 cut(s) 161, 184, 742, 764
AluI AGCT 4 cut(s) 161, 184, 742, 764
Alw21I GWGCWC 1 cut(s) 744
Alw26I GTCTC 1 cut(s) 599
AlwI GGATC 5 cut(s) 364, 372, 385, 447, 460
AoxI GGCC 1 cut(s) 128
ApeKI GCWGC 2 cut(s) 98, 764
ApoI RAATTY 2 cut(s) 332, 466
AsiGI ACCGGT 1 cut(s) 141
Asp700I GAANNNNTTC 1 cut(s) 281
AspLEI GCGC 1 cut(s) 680
AspS9I GGNCC 1 cut(s) 405
AsuC2I CCSGG 1 cut(s) 103
AsuHPI GGTGA 3 cut(s) 504, 584, 644
AvaII GGWCC 1 cut(s) 405
BamHI GGATCC 2 cut(s) 377, 452
BanII GRGCYC 1 cut(s) 744
BauI CACGAG 1 cut(s) 309
BbsI GAAGAC 2 cut(s) 274, 662
Bbv12I GWGCWC 1 cut(s) 744
BbvI GCAGC 2 cut(s) 110, 751
BccI CCATC 2 cut(s) 392, 443
BciT130I CCWGG 2 cut(s) 179, 596
BcnI CCSGG 1 cut(s) 103
BcoDI GTCTC 1 cut(s) 599
BcuI ACTAGT 2 cut(s) 383, 530
BfaI CTAG 2 cut(s) 384, 531
BglI GCCNNNNNGGC 2 cut(s) 127, 773
BglII AGATCT 2 cut(s) 234, 412
BisI GCNGC 2 cut(s) 99, 765
BlpI GCTNAGC 1 cut(s) 743
BlsI GCNGC 2 cut(s) 100, 766
BmcAI AGTACT 1 cut(s) 535
Bme1390I CCNGG 3 cut(s) 103, 179, 596
Bme18I GGWCC 1 cut(s) 405
BmgT120I GGNCC 1 cut(s) 405
BmiI GGNNCC 5 cut(s) 6, 293, 379, 427, 454
BmrFI CCNGG 3 cut(s) 103, 179, 596
BpiI GAAGAC 2 cut(s) 274, 662
BpmI CTGGAG 2 cut(s) 161, 201
Bpu10I CCTNAGC 1 cut(s) 703
Bpu1102I GCTNAGC 1 cut(s) 743
BpuEI CTTGAG 2 cut(s) 287, 758
BpuMI CCSGG 1 cut(s) 103
BsaJI CCNNGG 4 cut(s) 75, 570, 643, 666
BsaWI WCCGGW 3 cut(s) 141, 695, 758
Bsc4I CCNNNNNNNGG 2 cut(s) 141, 629
Bse118I RCCGGY 1 cut(s) 141
Bse1I ACTGG 2 cut(s) 436, 445
BseBI CCWGG 2 cut(s) 179, 596
BseDI CCNNGG 4 cut(s) 75, 570, 643, 666
BseGI GGATG 1 cut(s) 662
BseLI CCNNNNNNNGG 2 cut(s) 141, 629
BseMII CTCAG 2 cut(s) 717, 757
BseNI ACTGG 2 cut(s) 436, 445
BseRI GAGGAG 3 cut(s) 92, 499, 600
BseXI GCAGC 2 cut(s) 110, 751
BseYI CCCAGC 2 cut(s) 39, 63
BshFI GGCC 1 cut(s) 130
BshTI ACCGGT 1 cut(s) 141
BsiHKAI GWGCWC 1 cut(s) 744
BsiSI CCGG 4 cut(s) 102, 142, 696, 759
BslI CCNNNNNNNGG 2 cut(s) 141, 629
BsmAI GTCTC 1 cut(s) 599
BsnI GGCC 1 cut(s) 130
Bsp1286I GDGCHC 1 cut(s) 744
Bsp143I GATC 9 cut(s) 234, 264, 356, 364, 377, 412, 452, 460, 589
Bsp1720I GCTNAGC 1 cut(s) 743
Bsp19I CCATGG 1 cut(s) 570
BspACI CCGC 1 cut(s) 708
BspANI GGCC 1 cut(s) 130
BspCNI CTCAG 2 cut(s) 716, 756
BspLI GGNNCC 5 cut(s) 6, 293, 379, 427, 454
BspPI GGATC 5 cut(s) 364, 372, 385, 447, 460
BsrFI RCCGGY 1 cut(s) 141
BsrI ACTGG 2 cut(s) 436, 445
BssAI RCCGGY 1 cut(s) 141
BssECI CCNNGG 4 cut(s) 75, 570, 643, 666
BssMI GATC 9 cut(s) 234, 264, 356, 364, 377, 412, 452, 460, 589
BssSI CACGAG 1 cut(s) 309
BssT1I CCWWGG 2 cut(s) 570, 643
Bst2BI CACGAG 1 cut(s) 309
Bst2UI CCWGG 2 cut(s) 179, 596
Bst4CI ACNGT 2 cut(s) 632, 719
Bst6I CTCTTC 1 cut(s) 615
BstDEI CTNAG 4 cut(s) 167, 703, 743, 777
BstDSI CCRYGG 1 cut(s) 570
BstEII GGTNACC 1 cut(s) 647
BstF5I GGATG 1 cut(s) 662
BstHHI GCGC 1 cut(s) 680
BstKTI GATC 9 cut(s) 237, 267, 359, 367, 380, 415, 455, 463, 592
BstMAI GTCTC 1 cut(s) 599
BstMBI GATC 9 cut(s) 234, 264, 356, 364, 377, 412, 452, 460, 589
BstMWI GCNNNNNNNGC 2 cut(s) 127, 773
BstNI CCWGG 2 cut(s) 179, 596
BstPI GGTNACC 1 cut(s) 647
BstSCI CCNGG 3 cut(s) 101, 177, 594
BstV1I GCAGC 2 cut(s) 110, 751
BstV2I GAAGAC 2 cut(s) 274, 662
BstX2I RGATCY 5 cut(s) 234, 356, 377, 412, 452
BstYI RGATCY 5 cut(s) 234, 356, 377, 412, 452
BsuRI GGCC 1 cut(s) 130
BtgI CCRYGG 1 cut(s) 570
BtsCI GGATG 1 cut(s) 662
BtsIMutI CAGTG 2 cut(s) 429, 724
CfoI GCGC 1 cut(s) 680
Cfr10I RCCGGY 1 cut(s) 141
Cfr13I GGNCC 1 cut(s) 405
Csp6I GTAC 2 cut(s) 444, 534
CspAI ACCGGT 1 cut(s) 141
CviAII CATG 2 cut(s) 571, 625
CviQI GTAC 2 cut(s) 444, 534
DdeI CTNAG 4 cut(s) 167, 703, 743, 777
DpnI GATC 9 cut(s) 236, 266, 358, 366, 379, 414, 454, 462, 591
DpnII GATC 9 cut(s) 234, 264, 356, 364, 377, 412, 452, 460, 589
DrdI GACNNNNNNGTC 1 cut(s) 278
DseDI GACNNNNNNGTC 1 cut(s) 278
Eam1104I CTCTTC 1 cut(s) 615
EarI CTCTTC 1 cut(s) 615
Ecl136II GAGCTC 1 cut(s) 742
Eco130I CCWWGG 2 cut(s) 570, 643
Eco24I GRGCYC 1 cut(s) 744
Eco47I GGWCC 1 cut(s) 405
Eco53kI GAGCTC 1 cut(s) 742
Eco91I GGTNACC 1 cut(s) 647
EcoICRI GAGCTC 1 cut(s) 742
EcoO65I GGTNACC 1 cut(s) 647
EcoRII CCWGG 2 cut(s) 177, 594
EcoT14I CCWWGG 2 cut(s) 570, 643
EcoT38I GRGCYC 1 cut(s) 744
ErhI CCWWGG 2 cut(s) 570, 643
FaeI CATG 2 cut(s) 574, 628
FalI AAGNNNNNCTT 2 cut(s) 266, 298
FatI CATG 2 cut(s) 570, 624
FauNDI CATATG 1 cut(s) 682
Fnu4HI GCNGC 2 cut(s) 99, 765
FokI GGATG 1 cut(s) 649
FriOI GRGCYC 1 cut(s) 744
Fsp4HI GCNGC 2 cut(s) 99, 765
FspBI CTAG 2 cut(s) 384, 531
GlaI GCGC 1 cut(s) 679
GluI GCNGC 2 cut(s) 99, 765
GsaI CCCAGC 2 cut(s) 43, 67
GsuI CTGGAG 2 cut(s) 161, 201
HaeIII GGCC 1 cut(s) 130
HapII CCGG 4 cut(s) 102, 142, 696, 759
HhaI GCGC 1 cut(s) 680
Hin1II CATG 2 cut(s) 574, 628
Hin6I GCGC 1 cut(s) 678
HinP1I GCGC 1 cut(s) 678
HincII GTYRAC 1 cut(s) 496
HindII GTYRAC 1 cut(s) 496
HinfI GANTC 4 cut(s) 148, 171, 226, 607
HpaII CCGG 4 cut(s) 102, 142, 696, 759
HphI GGTGA 3 cut(s) 504, 584, 644
Hpy166II GTNNAC 2 cut(s) 147, 496
Hpy188I TCNGA 2 cut(s) 339, 667
Hpy188III TCNNGA 7 cut(s) 218, 230, 295, 311, 362, 604, 737
Hpy8I GTNNAC 2 cut(s) 147, 496
HpyAV CCTTC 4 cut(s) 251, 322, 526, 665
HpyCH4III ACNGT 2 cut(s) 632, 719
HpyCH4IV ACGT 3 cut(s) 279, 342, 639
HpyCH4V TGCA 3 cut(s) 19, 250, 628
HpyF10VI GCNNNNNNNGC 2 cut(s) 127, 773
HpyF3I CTNAG 4 cut(s) 167, 703, 743, 777
HpySE526I ACGT 3 cut(s) 279, 342, 639
Hsp92II CATG 2 cut(s) 574, 628
HspAI GCGC 1 cut(s) 678
Kzo9I GATC 9 cut(s) 234, 264, 356, 364, 377, 412, 452, 460, 589
LmnI GCTCC 2 cut(s) 181, 761
Lsp1109I GCAGC 2 cut(s) 110, 751
MaeI CTAG 2 cut(s) 384, 531
MaeII ACGT 3 cut(s) 279, 342, 639
MaeIII GTNAC 3 cut(s) 647, 686, 710
MalI GATC 9 cut(s) 236, 266, 358, 366, 379, 414, 454, 462, 591
MboI GATC 9 cut(s) 234, 264, 356, 364, 377, 412, 452, 460, 589
MboII GAAGA 3 cut(s) 274, 602, 667
MflI RGATCY 5 cut(s) 234, 356, 377, 412, 452
MhlI GDGCHC 1 cut(s) 744
MluCI AATT 3 cut(s) 332, 370, 466
MlyI GAGTC 2 cut(s) 142, 601
MroXI GAANNNNTTC 1 cut(s) 281
MseI TTAA 1 cut(s) 204
MspI CCGG 4 cut(s) 102, 142, 696, 759
MspR9I CCNGG 3 cut(s) 103, 179, 596
MvaI CCWGG 2 cut(s) 179, 596
MwoI GCNNNNNNNGC 2 cut(s) 127, 773
NciI CCSGG 1 cut(s) 103
NcoI CCATGG 1 cut(s) 570
NdeI CATATG 1 cut(s) 682
NdeII GATC 9 cut(s) 234, 264, 356, 364, 377, 412, 452, 460, 589
NlaIII CATG 2 cut(s) 574, 628
NlaIV GGNNCC 5 cut(s) 6, 293, 379, 427, 454
PcsI WCGNNNNNNNCGW 1 cut(s) 636
PdmI GAANNNNTTC 1 cut(s) 281
PfeI GAWTC 2 cut(s) 171, 226
PfoI TCCNGGA 1 cut(s) 177
PinAI ACCGGT 1 cut(s) 141
PkrI GCNGC 2 cut(s) 100, 766
PleI GAGTC 2 cut(s) 142, 601
PpsI GAGTC 2 cut(s) 142, 601
Psp124BI GAGCTC 1 cut(s) 744
Psp1406I AACGTT 1 cut(s) 342
Psp6I CCWGG 2 cut(s) 177, 594
PspEI GGTNACC 1 cut(s) 647
PspFI CCCAGC 2 cut(s) 39, 63
PspGI CCWGG 2 cut(s) 177, 594
PspN4I GGNNCC 5 cut(s) 6, 293, 379, 427, 454
PspPI GGNCC 1 cut(s) 405
PsuI RGATCY 5 cut(s) 234, 356, 377, 412, 452
RsaI GTAC 2 cut(s) 445, 535
RsaNI GTAC 2 cut(s) 444, 534
SacI GAGCTC 1 cut(s) 744
SaqAI TTAA 1 cut(s) 204
SatI GCNGC 2 cut(s) 99, 765
Sau3AI GATC 9 cut(s) 234, 264, 356, 364, 377, 412, 452, 460, 589
Sau96I GGNCC 1 cut(s) 405
ScaI AGTACT 1 cut(s) 535
SchI GAGTC 2 cut(s) 142, 601
ScrFI CCNGG 3 cut(s) 103, 179, 596
SduI GDGCHC 1 cut(s) 744
SinI GGWCC 1 cut(s) 405
SmlI CTYRAG 2 cut(s) 302, 737
SmoI CTYRAG 2 cut(s) 302, 737
SpeI ACTAGT 2 cut(s) 383, 530
Sse9I AATT 3 cut(s) 332, 370, 466
SsiI CCGC 1 cut(s) 708
SspMI CTAG 2 cut(s) 384, 531
SstI GAGCTC 1 cut(s) 744
StyD4I CCNGG 3 cut(s) 101, 177, 594
StyI CCWWGG 2 cut(s) 570, 643
TaaI ACNGT 2 cut(s) 632, 719
TaiI ACGT 3 cut(s) 282, 345, 642
TaqI TCGA 3 cut(s) 267, 363, 603
TasI AATT 3 cut(s) 332, 370, 466
TatI WGTACW 1 cut(s) 533
TfiI GAWTC 2 cut(s) 171, 226
Tru1I TTAA 1 cut(s) 204
Tru9I TTAA 1 cut(s) 204
TscAI CASTG 2 cut(s) 436, 724
TseI GCWGC 2 cut(s) 98, 764
TspDTI ATGAA 1 cut(s) 233
TspRI CASTG 2 cut(s) 436, 724
VpaK11BI GGWCC 1 cut(s) 405
XapI RAATTY 2 cut(s) 332, 466
XmnI GAANNNNTTC 1 cut(s) 281
XspI CTAG 2 cut(s) 384, 531
ZrmI AGTACT 1 cut(s) 535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.