Rh4BG012300
WRKY Family

protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0018019
Physical Location & Seq
Forward (+)
1710592 .. 1712634
2043 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG012300.1

Sequence Viewer

Length: 1734 bp
ATGTATATCTTTATGACCGAGGAGGAGGCCAACAAGAAGGTCTTGCAGTACGAGAATGTCCTGGTGACAAACAGAGAGCTTCATATTCTTAAAGGCCCACGCTTTCTAAACGACATCATCATCGAATTCTACTTCCTCCATATTTCTAATGCCCTCCTTCAATCCCATGAGTCTTTCCGAGATGACATCCTTCTGGTTTCGCCTGCCCTCTCATTCTTTCTGCAGCAGGATTTTGGGGATGACTCTCATGTCAAAGACTTTGCGGAGTCCAACAAAGTAGATAGCAAGCAGGTTGTAATGTTTACGGTGAATAACAACCTAGATGCCGGCAGGAACGACGGAGGAAGCCACTGGAGCTTGCTGGTGTACTACAGAAAATCAAATACATTCGTACACTATGACAGCTTGGGATGTAATGGTTCTTTTGCTAAGAAGCTTTTCCGAGCAGTCAAGAAACATGTGGCAACACCAGCTGAGAGTAGCTCCACCACTGGTACAATTGTTCGTGGAGATGTGGATTCTACCAATACTGATGCAATCAATATTGGAGATGTGGATTCTAACAATACTACGACAACTAATATTGGAGACGTGGACTCTAACAATACTAGTACAATCAATGGAGATAGAGATTCTACCGATACTACTATGGTCGCAGATTCTACCAATACTAGTGTGGTCGATGGAGATTGTACCATGATCGATGGAGATACTACTAATACTAGTATGATGGATGCAGATTTTTCTTCTAGTAGTAGTAGAGTTGATGGAGATTATACTAATTGTACTGGAGTTCATGGACATTGTACAAGGTTTGATAATGATGAAAACCAGTTGCAACTATATAATCAGAAGAAACAAAGGATGCGACGGAATTGGGAAGAAGAGGATATGCCCTGGAGGTATCATCAACGTGGTTTTCGCCGTAGTTGGTTGTGGAATGGGGGTTTTATGAACTCGAGGTCTGGTTATACTCGTTGGGATAGGGATGCTCGTGGGTTTCTTTTGCCAATAGGTTACCAGTATCATGAGGAAGATGATGATTTTAAAGAAGAGGGATGCATGCCGAAGCAGAAGAATTTTTATGACTGTGGTTTGTACGTGATGGAAATTGCTAGGGTGGTCTGTGAATGGTACTACCTTGATGACGAAGCTAAGCAGCAACAGCCGGAGCATCGAGGTTTTTCCTGTAAGTTGGGGGGTGGCGGCCGGTTTCCCAACATCAGTGAGTCTGTTCATACTTCTTTGGAATCCACAATGCGCGATATGCTCTTGTCCATCATTCAACAACGTGCACGCGAGTCTGGAATACATCTTGATTTATTTTCTTCTGATGATGATGAGTCCAAGGCTGACGATGTTCTTGGTTTACATTCTGATGAAAATGAGTCCAAGGATGATGACAGTGCTCCCCAATTAGGCTCCCAGCCCAAGTATGATGATGAAGAAACACCGGTGGATAGTGTTGTTCGTGATTTGTTTTATGGTAATAAAGCCAATGAAAAGAACCACCAATTCGGCCCCCAGCCCCGGCCCCAACAATATAAGTCGGAGGATGAAACACTCGAGGATTGTGGTGGTGTTGCTGCTCCCCCTCCACAATCTGTATCCTCAATTTCAGAAGATGAGCCAGAAGAAATTTGGGATCGTATACTAGAGGACAGGTGCTCATTGTTGAGTAAATCTAGCAATTATCTAGAAGGAGATGATCTCCTCGCTAAAAAACGAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 WRKY

Protein Analysis

577

Amino Acids

65.5

Weight (kDa)

4.65

Isoelectric Point (pI)

53.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018019)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g01090
rosa_chinensis RchiOBHm_Chr4g0387651
rosa_laevigata RLG00000010103
rosa_multiflora Rmu_sc0003953.1_g000007
rosa_roxburghii Rroxscaffold_5G00334320
rosa_rugosa Rorug03G0312200
rosa_samantha Rh4AG017500 Rh4BG012300 Rh4CG018700 Rh4DG013300
rosa_wichuraiana Rw4G001130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 248
Acc36I ACCTGC 1 cut(s) 280
AccI GTMKAC 1 cut(s) 1651
AccII CGCG 2 cut(s) 1263, 1299
AciI CCGC 2 cut(s) 263, 1206
AclWI GGATC 1 cut(s) 1653
AcoI YGGCCR 1 cut(s) 1207
AcsI RAATTY 3 cut(s) 125, 1078, 1638
AfiI CCNNNNNNNGG 2 cut(s) 1418, 1530
AflIII ACRYGT 1 cut(s) 457
AgeI ACCGGT 1 cut(s) 1453
AgsI TTSAA 2 cut(s) 161, 1286
AhlI ACTAGT 3 cut(s) 608, 671, 722
AjiI CACGTC 1 cut(s) 592
AjnI CCWGG 2 cut(s) 60, 896
AluBI AGCT 7 cut(s) 79, 357, 405, 436, 473, 483, 1154
AluI AGCT 7 cut(s) 79, 357, 405, 436, 473, 483, 1154
Alw21I GWGCWC 3 cut(s) 1297, 1411, 1670
Alw26I GTCTC 1 cut(s) 582
Alw44I GTGCAC 1 cut(s) 1293
AlwI GGATC 1 cut(s) 1653
Ama87I CYCGRG 2 cut(s) 958, 1565
AoxI GGCC 5 cut(s) 27, 94, 1207, 1519, 1532
ApaLI GTGCAC 1 cut(s) 1293
ApeKI GCWGC 3 cut(s) 223, 1159, 1586
ApoI RAATTY 3 cut(s) 125, 1078, 1638
AsiGI ACCGGT 1 cut(s) 1453
Asp700I GAANNNNTTC 1 cut(s) 437
AspLEI GCGC 1 cut(s) 1263
AspS9I GGNCC 3 cut(s) 95, 1520, 1533
AsuC2I CCSGG 1 cut(s) 1531
AsuHPI GGTGA 2 cut(s) 76, 319
AvaI CYCGRG 2 cut(s) 958, 1565
BaeGI GKGCMC 1 cut(s) 1297
BaeI ACNNNNGTAYC 2 cut(s) 486, 519
BauI CACGAG 1 cut(s) 993
Bbv12I GWGCWC 3 cut(s) 1297, 1411, 1670
BbvI GCAGC 3 cut(s) 235, 1171, 1573
BccI CCATC 6 cut(s) 677, 698, 724, 761, 1099, 1286
BceAI ACGGC 1 cut(s) 909
BciT130I CCWGG 2 cut(s) 62, 898
BciVI GTATCC 1 cut(s) 1618
BcnI CCSGG 1 cut(s) 1531
BcoDI GTCTC 1 cut(s) 582
BcuI ACTAGT 3 cut(s) 608, 671, 722
BfaI CTAG 9 cut(s) 320, 609, 672, 723, 750, 1116, 1655, 1686, 1697
BfmI CTRYAG 2 cut(s) 221, 370
BfuAI ACCTGC 1 cut(s) 280
BfuI GTATCC 1 cut(s) 1618
BisI GCNGC 4 cut(s) 224, 1160, 1207, 1587
BlpI GCTNAGC 1 cut(s) 1155
BlsI GCNGC 4 cut(s) 225, 1161, 1208, 1588
Bme1390I CCNGG 3 cut(s) 62, 898, 1531
BmeT110I CYCGRG 2 cut(s) 958, 1565
BmgBI CACGTC 1 cut(s) 592
BmgT120I GGNCC 3 cut(s) 95, 1520, 1533
BmiI GGNNCC 3 cut(s) 1423, 1522, 1535
BmrFI CCNGG 3 cut(s) 62, 898, 1531
BmsI GCATC 7 cut(s) 313, 523, 724, 855, 979, 1049, 1183
BplI GAGNNNNNCTC 4 cut(s) 467, 499, 1695, 1727
BpmI CTGGAG 3 cut(s) 373, 810, 919
Bpu1102I GCTNAGC 1 cut(s) 1155
BpuMI CCSGG 1 cut(s) 1531
Bsa29I ATCGAT 1 cut(s) 702
BsaAI YACGTR 1 cut(s) 1102
BsaJI CCNNGG 5 cut(s) 18, 896, 1347, 1392, 1529
BsaWI WCCGGW 1 cut(s) 1453
Bsc4I CCNNNNNNNGG 2 cut(s) 1418, 1530
Bse118I RCCGGY 3 cut(s) 326, 1209, 1453
Bse1I ACTGG 5 cut(s) 356, 496, 793, 832, 1021
BseBI CCWGG 2 cut(s) 62, 898
BseCI ATCGAT 1 cut(s) 702
BseDI CCNNGG 5 cut(s) 18, 896, 1347, 1392, 1529
BseGI GGATG 9 cut(s) 186, 244, 416, 739, 870, 994, 1064, 1402, 1561
BseLI CCNNNNNNNGG 2 cut(s) 1418, 1530
BseMII CTCAG 1 cut(s) 465
BseNI ACTGG 5 cut(s) 356, 496, 793, 832, 1021
BseRI GAGGAG 3 cut(s) 35, 38, 1703
BseSI GKGCMC 1 cut(s) 1297
BseX3I CGGCCG 1 cut(s) 1207
BseXI GCAGC 3 cut(s) 235, 1171, 1573
BseYI CCCAGC 2 cut(s) 1425, 1524
Bsh1236I CGCG 2 cut(s) 1263, 1299
Bsh1285I CGRYCG 1 cut(s) 1210
BshFI GGCC 5 cut(s) 29, 96, 1209, 1521, 1534
BshTI ACCGGT 1 cut(s) 1453
BshVI ATCGAT 1 cut(s) 702
BsiEI CGRYCG 1 cut(s) 1210
BsiHKAI GWGCWC 3 cut(s) 1297, 1411, 1670
BsiHKCI CYCGRG 2 cut(s) 958, 1565
BsiSI CCGG 5 cut(s) 327, 1169, 1210, 1454, 1531
BslI CCNNNNNNNGG 2 cut(s) 1418, 1530
BsmAI GTCTC 1 cut(s) 582
BsmBI CGTCTC 1 cut(s) 582
BsnI GGCC 5 cut(s) 29, 96, 1209, 1521, 1534
BsoBI CYCGRG 2 cut(s) 958, 1565
Bsp1286I GDGCHC 3 cut(s) 1297, 1411, 1670
Bsp1407I TGTACA 1 cut(s) 806
Bsp143I GATC 3 cut(s) 699, 1645, 1708
Bsp1720I GCTNAGC 1 cut(s) 1155
BspACI CCGC 2 cut(s) 263, 1206
BspANI GGCC 5 cut(s) 29, 96, 1209, 1521, 1534
BspCNI CTCAG 1 cut(s) 466
BspDI ATCGAT 1 cut(s) 702
BspFNI CGCG 2 cut(s) 1263, 1299
BspHI TCATGA 1 cut(s) 1027
BspLI GGNNCC 3 cut(s) 1423, 1522, 1535
BspMAI CTGCAG 1 cut(s) 225
BspMI ACCTGC 1 cut(s) 280
BspPI GGATC 1 cut(s) 1653
BsrFI RCCGGY 3 cut(s) 326, 1209, 1453
BsrGI TGTACA 1 cut(s) 806
BsrI ACTGG 5 cut(s) 356, 496, 793, 832, 1021
BssAI RCCGGY 3 cut(s) 326, 1209, 1453
BssECI CCNNGG 5 cut(s) 18, 896, 1347, 1392, 1529
BssMI GATC 3 cut(s) 699, 1645, 1708
BssNAI GTATAC 1 cut(s) 1652
BssSI CACGAG 1 cut(s) 993
BssT1I CCWWGG 2 cut(s) 1347, 1392
Bst1107I GTATAC 1 cut(s) 1652
Bst2BI CACGAG 1 cut(s) 993
Bst2UI CCWGG 2 cut(s) 62, 898
Bst4CI ACNGT 3 cut(s) 307, 1091, 1406
Bst6I CTCTTC 2 cut(s) 879, 1047
BstAUI TGTACA 1 cut(s) 806
BstBAI YACGTR 1 cut(s) 1102
BstC8I GCNNGC 6 cut(s) 204, 287, 328, 359, 1064, 1297
BstDEI CTNAG 3 cut(s) 429, 474, 1155
BstEII GGTNACC 1 cut(s) 1016
BstF5I GGATG 9 cut(s) 186, 244, 416, 739, 870, 994, 1064, 1402, 1561
BstFNI CGCG 2 cut(s) 1263, 1299
BstHHI GCGC 1 cut(s) 1263
BstKTI GATC 3 cut(s) 702, 1648, 1711
BstMAI GTCTC 1 cut(s) 582
BstMBI GATC 3 cut(s) 699, 1645, 1708
BstMCI CGRYCG 1 cut(s) 1210
BstMWI GCNNNNNNNGC 4 cut(s) 354, 470, 1165, 1267
BstNI CCWGG 2 cut(s) 62, 898
BstNSI RCATGY 2 cut(s) 461, 1066
BstPI GGTNACC 1 cut(s) 1016
BstSCI CCNGG 3 cut(s) 60, 896, 1529
BstSFI CTRYAG 2 cut(s) 221, 370
BstSLI GKGCMC 1 cut(s) 1297
BstUI CGCG 2 cut(s) 1263, 1299
BstV1I GCAGC 3 cut(s) 235, 1171, 1573
BstZ17I GTATAC 1 cut(s) 1652
BstZI CGGCCG 1 cut(s) 1207
Bsu15I ATCGAT 1 cut(s) 702
BsuI GTATCC 1 cut(s) 1618
BsuRI GGCC 5 cut(s) 29, 96, 1209, 1521, 1534
BsuTUI ATCGAT 1 cut(s) 702
BtrI CACGTC 1 cut(s) 592
BtsCI GGATG 9 cut(s) 186, 244, 416, 739, 870, 994, 1064, 1402, 1561
BtsIMutI CAGTG 4 cut(s) 349, 489, 1231, 1411
BveI ACCTGC 1 cut(s) 280
Cac8I GCNNGC 6 cut(s) 204, 287, 328, 359, 1064, 1297
CciI TCATGA 1 cut(s) 1027
CfoI GCGC 1 cut(s) 1263
Cfr10I RCCGGY 3 cut(s) 326, 1209, 1453
Cfr13I GGNCC 3 cut(s) 95, 1520, 1533
ClaI ATCGAT 1 cut(s) 702
CspAI ACCGGT 1 cut(s) 1453
CviAII CATG 8 cut(s) 167, 248, 458, 697, 797, 1028, 1063, 1731
DdeI CTNAG 3 cut(s) 429, 474, 1155
DpnI GATC 3 cut(s) 701, 1647, 1710
DpnII GATC 3 cut(s) 699, 1645, 1708
DraI TTTAAA 1 cut(s) 1048
DrdI GACNNNNNNGTC 1 cut(s) 248
DseDI GACNNNNNNGTC 1 cut(s) 248
EaeI YGGCCR 1 cut(s) 1207
EagI CGGCCG 1 cut(s) 1207
Eam1104I CTCTTC 2 cut(s) 879, 1047
EarI CTCTTC 2 cut(s) 879, 1047
EclXI CGGCCG 1 cut(s) 1207
Eco130I CCWWGG 2 cut(s) 1347, 1392
Eco52I CGGCCG 1 cut(s) 1207
Eco88I CYCGRG 2 cut(s) 958, 1565
Eco91I GGTNACC 1 cut(s) 1016
EcoO65I GGTNACC 1 cut(s) 1016
EcoRI GAATTC 1 cut(s) 125
EcoRII CCWGG 2 cut(s) 60, 896
EcoT14I CCWWGG 2 cut(s) 1347, 1392
EcoT22I ATGCAT 1 cut(s) 1064
ErhI CCWWGG 2 cut(s) 1347, 1392
Esp3I CGTCTC 1 cut(s) 582
FaeI CATG 8 cut(s) 170, 251, 461, 700, 800, 1031, 1066, 1734
FalI AAGNNNNNCTT 2 cut(s) 26, 58
FatI CATG 8 cut(s) 166, 247, 457, 696, 796, 1027, 1062, 1730
FblI GTMKAC 1 cut(s) 1651
Fnu4HI GCNGC 4 cut(s) 224, 1160, 1207, 1587
FokI GGATG 9 cut(s) 173, 251, 423, 746, 877, 1001, 1071, 1409, 1568
Fsp4HI GCNGC 4 cut(s) 224, 1160, 1207, 1587
FspBI CTAG 9 cut(s) 320, 609, 672, 723, 750, 1116, 1655, 1686, 1697
GlaI GCGC 1 cut(s) 1262
GluI GCNGC 4 cut(s) 224, 1160, 1207, 1587
GsaI CCCAGC 2 cut(s) 1429, 1528
GsuI CTGGAG 3 cut(s) 373, 810, 919
HaeIII GGCC 5 cut(s) 29, 96, 1209, 1521, 1534
HapII CCGG 5 cut(s) 327, 1169, 1210, 1454, 1531
HhaI GCGC 1 cut(s) 1263
Hin1II CATG 8 cut(s) 170, 251, 461, 700, 800, 1031, 1066, 1734
Hin6I GCGC 1 cut(s) 1261
HinP1I GCGC 1 cut(s) 1261
HindIII AAGCTT 1 cut(s) 434
HpaII CCGG 5 cut(s) 327, 1169, 1210, 1454, 1531
HphI GGTGA 2 cut(s) 76, 319
Hpy166II GTNNAC 7 cut(s) 303, 367, 394, 595, 1295, 1370, 1652
Hpy188I TCNGA 7 cut(s) 179, 443, 852, 1333, 1378, 1552, 1621
Hpy188III TCNNGA 6 cut(s) 451, 1028, 1305, 1316, 1472, 1697
Hpy8I GTNNAC 7 cut(s) 303, 367, 394, 595, 1295, 1370, 1652
Hpy99I CGWCG 2 cut(s) 341, 873
HpyAV CCTTC 4 cut(s) 31, 167, 200, 1694
HpyCH4III ACNGT 3 cut(s) 307, 1091, 1406
HpyCH4IV ACGT 4 cut(s) 591, 913, 1101, 1291
HpyCH4V TGCA 7 cut(s) 46, 223, 536, 737, 838, 1062, 1295
HpyF10VI GCNNNNNNNGC 4 cut(s) 354, 470, 1165, 1267
HpyF3I CTNAG 3 cut(s) 429, 474, 1155
HpySE526I ACGT 4 cut(s) 591, 913, 1101, 1291
Hsp92II CATG 8 cut(s) 170, 251, 461, 700, 800, 1031, 1066, 1734
HspAI GCGC 1 cut(s) 1261
KroI GCCGGC 1 cut(s) 326
KroNI GCCGGC 1 cut(s) 328
Kzo9I GATC 3 cut(s) 699, 1645, 1708
LmnI GCTCC 6 cut(s) 354, 488, 1171, 1414, 1427, 1594
Lsp1109I GCAGC 3 cut(s) 235, 1171, 1573
LweI GCATC 7 cut(s) 313, 523, 724, 855, 979, 1049, 1183
MaeI CTAG 9 cut(s) 320, 609, 672, 723, 750, 1116, 1655, 1686, 1697
MaeII ACGT 4 cut(s) 591, 913, 1101, 1291
MaeIII GTNAC 2 cut(s) 64, 1016
MalI GATC 3 cut(s) 701, 1647, 1710
MboI GATC 3 cut(s) 699, 1645, 1708
MfeI CAATTG 1 cut(s) 498
MhlI GDGCHC 3 cut(s) 1297, 1411, 1670
MlyI GAGTC 8 cut(s) 179, 236, 275, 590, 1238, 1310, 1352, 1397
MmeI TCCRAC 2 cut(s) 294, 1530
Mph1103I ATGCAT 1 cut(s) 1064
MroNI GCCGGC 1 cut(s) 326
MroXI GAANNNNTTC 1 cut(s) 437
MseI TTAA 2 cut(s) 90, 1047
MslI CAYNNNNRTG 2 cut(s) 912, 1377
MspA1I CMGCKG 1 cut(s) 473
MspI CCGG 5 cut(s) 327, 1169, 1210, 1454, 1531
MspR9I CCNGG 3 cut(s) 62, 898, 1531
MunI CAATTG 1 cut(s) 498
MvaI CCWGG 2 cut(s) 62, 898
MvnI CGCG 2 cut(s) 1263, 1299
MwoI GCNNNNNNNGC 4 cut(s) 354, 470, 1165, 1267
NaeI GCCGGC 1 cut(s) 328
NciI CCSGG 1 cut(s) 1531
NdeII GATC 3 cut(s) 699, 1645, 1708
NgoMIV GCCGGC 1 cut(s) 326
NlaIII CATG 8 cut(s) 170, 251, 461, 700, 800, 1031, 1066, 1734
NlaIV GGNNCC 3 cut(s) 1423, 1522, 1535
NmuCI GTSAC 1 cut(s) 64
NsiI ATGCAT 1 cut(s) 1064
NspI RCATGY 2 cut(s) 461, 1066
PaeI GCATGC 1 cut(s) 1066
PaeR7I CTCGAG 2 cut(s) 958, 1565
PagI TCATGA 1 cut(s) 1027
PciI ACATGT 1 cut(s) 457
PdiI GCCGGC 1 cut(s) 328
PdmI GAANNNNTTC 1 cut(s) 437
PfeI GAWTC 5 cut(s) 518, 557, 632, 659, 1250
PinAI ACCGGT 1 cut(s) 1453
PkrI GCNGC 4 cut(s) 225, 1161, 1208, 1588
PleI GAGTC 8 cut(s) 178, 236, 274, 590, 1237, 1309, 1351, 1396
PpsI GAGTC 8 cut(s) 178, 236, 274, 590, 1237, 1309, 1351, 1396
Ppu21I YACGTR 1 cut(s) 1102
PscI ACATGT 1 cut(s) 457
Psp6I CCWGG 2 cut(s) 60, 896
PspEI GGTNACC 1 cut(s) 1016
PspFI CCCAGC 2 cut(s) 1425, 1524
PspGI CCWGG 2 cut(s) 60, 896
PspN4I GGNNCC 3 cut(s) 1423, 1522, 1535
PspPI GGNCC 3 cut(s) 95, 1520, 1533
PspXI VCTCGAGB 2 cut(s) 958, 1565
PstI CTGCAG 1 cut(s) 225
PvuII CAGCTG 1 cut(s) 473
RseI CAYNNNNRTG 2 cut(s) 912, 1377
SaqAI TTAA 2 cut(s) 90, 1047
SatI GCNGC 4 cut(s) 224, 1160, 1207, 1587
Sau3AI GATC 3 cut(s) 699, 1645, 1708
Sau96I GGNCC 3 cut(s) 95, 1520, 1533
SchI GAGTC 8 cut(s) 179, 236, 275, 590, 1238, 1310, 1352, 1397
ScrFI CCNGG 3 cut(s) 62, 898, 1531
SduI GDGCHC 3 cut(s) 1297, 1411, 1670
SfaNI GCATC 7 cut(s) 313, 523, 724, 855, 979, 1049, 1183
SfcI CTRYAG 2 cut(s) 221, 370
Sfr274I CTCGAG 2 cut(s) 958, 1565
SgrAI CRCCGGYG 1 cut(s) 1453
SlaI CTCGAG 2 cut(s) 958, 1565
SmiMI CAYNNNNRTG 2 cut(s) 912, 1377
SmlI CTYRAG 2 cut(s) 958, 1565
SmoI CTYRAG 2 cut(s) 958, 1565
SpeI ACTAGT 3 cut(s) 608, 671, 722
SphI GCATGC 1 cut(s) 1066
SsiI CCGC 2 cut(s) 263, 1206
SspI AATATT 2 cut(s) 544, 583
SspMI CTAG 9 cut(s) 320, 609, 672, 723, 750, 1116, 1655, 1686, 1697
StyD4I CCNGG 3 cut(s) 60, 896, 1529
StyI CCWWGG 2 cut(s) 1347, 1392
TaaI ACNGT 3 cut(s) 307, 1091, 1406
TaiI ACGT 4 cut(s) 594, 916, 1104, 1294
TaqI TCGA 6 cut(s) 123, 681, 702, 959, 1177, 1566
TaqII GACCGA 1 cut(s) 32
TatI WGTACW 4 cut(s) 366, 611, 785, 806
TauI GCSGC 1 cut(s) 1209
TfiI GAWTC 5 cut(s) 518, 557, 632, 659, 1250
Tru1I TTAA 2 cut(s) 90, 1047
Tru9I TTAA 2 cut(s) 90, 1047
TscAI CASTG 4 cut(s) 356, 496, 1231, 1411
TseFI GTSAC 1 cut(s) 64
TseI GCWGC 3 cut(s) 223, 1159, 1586
Tsp45I GTSAC 1 cut(s) 64
TspDTI ATGAA 9 cut(s) 71, 785, 840, 968, 1226, 1395, 1458, 1515, 1572
TspGWI ACGGA 2 cut(s) 354, 886
TspRI CASTG 4 cut(s) 356, 496, 1231, 1411
VneI GTGCAC 1 cut(s) 1293
XapI RAATTY 3 cut(s) 125, 1078, 1638
XbaI TCTAGA 1 cut(s) 1696
XceI RCATGY 2 cut(s) 461, 1066
XcmI CCANNNNNNNNNTGG 2 cut(s) 673, 1638
XhoI CTCGAG 2 cut(s) 958, 1565
XmiI GTMKAC 1 cut(s) 1651
XmnI GAANNNNTTC 1 cut(s) 437
XspI CTAG 9 cut(s) 320, 609, 672, 723, 750, 1116, 1655, 1686, 1697
Zsp2I ATGCAT 1 cut(s) 1064
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.