FvH4_4g05100

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0016816
Physical Location & Seq
Forward (+)
4414496 .. 4417174
2679 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g05100.t1

Sequence Viewer

Length: 1893 bp
ATGAGCCTCTCCGCTACACGCAAGACATTCACCCTCAATACCTTCCGATATTGCACCTGTGCCACTGCCCTCCACCCATCGCCGGACCTCCACCACCTTCTCCAGCTCTCAATCACACACCGCTCTCTTCCACTCGCCCAACAATCCCACGCGCGAGTCCTCACTTATGGCCTCCACCAAAACCCATTCATCGCCACTAAGCTCATCTCCGCCTACGCCCAATTCAACCACCCAACCAACTCCAAGCTCGTCTTCGACTCCATTCACCCTAAAAACGTGTATCTCTGGAACTCAATGATCAGTGCCTACGTCAGAACCCGTCTTCACAACGAAGCTTTCAGTTTATTCATCGAAATGTTGTGTTCTGCATCCCCGGATGAGTTTACGTTTTCGACAATGGCGAAGGTTTCCGGCGAGGCTGGGAACTTGGCTACCGGAAAATGGGTTCATGGAAAGTGTGTGAGAGCTGGGTTTGTTTCGGATATGATTGTCGTGAATTCGGTGATGTCAATGTATTGTAAATGTGGGGAGTTTGGGGAGTGCAGGAAGGTGTTCGATGAAATGCCTGATAGAAATGTTGGGTCTTGGAATGTGTTGATCGGAGGGTTCGGGAGTGGCGGTGAGTGTGAATTTGGGGATGGGGTGTTGGAAATGGTGAAGGGTATGATGAGGAGTGGGTTGAGACCCGACGGGTTTACTGTTTCGAGTTTGTTGGCAATGTGTGGAGGGAAGGGTGTTGGGGGGTTGGGGTATGGGAGGGAGATGCATTGTTATGTTGTGAAGTATCGGTTGGAGTTGAGTTTGGGTTCGGATGTTCATCTTGGGTGTTGTTTGGTTGATATGTATTGTAGGAGTGGTAGAGTTGATTTGGGGAGACGGGTGTTTGAGGGTATGAAGTGTAGAAATGTGTATGCTTGGACGGCGATGGTCAATGGTTATGTGCAGAGTGGAGGTTCTGATGAAGGGTTGGTTCTTTTCAGGAAGATGCAGGTGAAAGATGGGATAGAACCCAATAGAGTATCACTTGTGAGTATTCTCCCGGGTTGTATTTCACATGCTGGTTTAACGGGCGGGAAACAAATTCATGGGTTTGCAATTAGGAAGGAGATGAATCATGATGTATCTTTATGCAATGCTTTGATTGATATGTATTGCAAATGTGGGAGCTTGGATTTTGCAAGGCGAGTGTTTGAGGATGACTCGTTTTGTAAGGATGCAATCTCTTGGAGTTCAATGATATCAGGGTATGGATTACATGGAAGGGGCGAGGAAGCCATTGTTTTGTATAATAAGATGGTTCGGCTTGGAATCAAACCGGACATGATAACCATTGTTGGGGTTCTTTCTGCCTGTAGCAGGTCAGGATTGGTAAATGAAGGCCTTTCAGTCTATAGCTCTGCAACAACTGATTATGGGATCAAACCAAGAGTGGAGATGTGTGCCTGTGTGGTGGATCTGTTAGGTCGATCAGGAGAGCTTAATCAAGCATTAAATTTCATCAAAACTATGCCTGTAGAAGCCAGTCCAAGTGTTTGGGGGGCTTTAGTTACTGCTTCTGTATTGCATGGGAACCGCGACATGCAAGATCTAGCTTATAAGTTCCTCATTCAACTAGAACCCGAAAATCCTTCAAACTATATCTCAGCATCAAATTTCTTTGCTTCTTCTAGAAGATGGGACATTGTAGCTGAAACACGAACAATGATGAAGGACAGAGGTTTGAGGAAGACACCTGGGTGCAGTTGGATCAGCATTACTGGTAAGACTCATTCTTTCTTTGTTGCTGATAAAGTACATCCTTGTTGTGATTCAATCTATGAGATGCTTGACTTCCTCATCTTAGTAATTAAGGGAGCCCCCAATTTCCATGACTTAGTAGAATATTCAGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

631

Amino Acids

69.44

Weight (kDa)

8.2

Isoelectric Point (pI)

33.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 91 - 134 3.9e-09 PPR repeat family
PPR PF01535 95 - 120 1.6e-06 PPR repeat
PPR PF01535 165 - 193 4.1e-06 PPR repeat
PPR_2 PF13041 405 - 452 1.7e-09 PPR repeat family
PPR PF01535 407 - 437 5.7e-08 PPR repeat
E_motif PF20431 522 - 583 2.9e-15 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016816)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g05100
malus_domestica MD13G1224800.v1.1
prunus_persica Prupe.1G051400_v2.0.a1
pyrus_communis pycom13g19840
rosa_chinensis RchiOBHm_Chr4g0396021
rosa_laevigata RLG00000009580
rosa_multiflora Rmu_sc0030438.1_g000001 Rmu_sc0031838.1_g000001
rosa_roxburghii Rroxscaffold_5G00340810
rosa_rugosa Rorug03G0352900
rosa_samantha Rh4BG063500 Rh4CG070100 Rh4DG060000
rosa_wichuraiana Rw4G005240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1596
AarI CACCTGC 1 cut(s) 979
Acc36I ACCTGC 2 cut(s) 979, 1347
AccBSI CCGCTC 1 cut(s) 123
AccII CGCG 3 cut(s) 152, 154, 1575
AciI CCGC 6 cut(s) 12, 121, 210, 618, 1071, 1573
AclWI GGATC 3 cut(s) 1424, 1461, 1754
AcsI RAATTY 5 cut(s) 496, 629, 1080, 1492, 1651
AfaI GTAC 1 cut(s) 1794
AfiI CCNNNNNNNGG 4 cut(s) 82, 441, 1335, 1356
AflIII ACRYGT 1 cut(s) 276
AgsI TTSAA 5 cut(s) 226, 1233, 1610, 1632, 1812
AjnI CCWGG 1 cut(s) 1732
AjuI GAANNNNNNNTTGG 4 cut(s) 236, 268, 773, 805
AleI CACNNNNGTG 1 cut(s) 1735
AloI GAACNNNNNNTCC 2 cut(s) 429, 461
Alw26I GTCTC 2 cut(s) 676, 868
AlwI GGATC 3 cut(s) 1424, 1461, 1754
Ama87I CYCGRG 1 cut(s) 1039
AoxI GGCC 2 cut(s) 169, 1378
ApoI RAATTY 5 cut(s) 496, 629, 1080, 1492, 1651
Asp700I GAANNNNTTC 1 cut(s) 551
AspLEI GCGC 1 cut(s) 154
AspS9I GGNCC 1 cut(s) 85
AsuC2I CCSGG 3 cut(s) 374, 1040, 1041
AsuHPI GGTGA 6 cut(s) 22, 257, 514, 632, 667, 1003
AvaI CYCGRG 1 cut(s) 1039
AvaII GGWCC 1 cut(s) 85
BanII GRGCYC 1 cut(s) 1858
BbsI GAAGAC 3 cut(s) 244, 314, 1733
BccI CCATC 6 cut(s) 85, 632, 919, 992, 1288, 1668
BceAI ACGGC 1 cut(s) 936
BciT130I CCWGG 1 cut(s) 1734
BclI TGATCA 1 cut(s) 297
BcnI CCSGG 3 cut(s) 374, 1040, 1041
BcoDI GTCTC 2 cut(s) 676, 868
BfaI CTAG 4 cut(s) 1589, 1613, 1668, 1891
BfmI CTRYAG 3 cut(s) 1351, 1390, 1512
BfuAI ACCTGC 2 cut(s) 979, 1347
BglII AGATCT 1 cut(s) 1585
Bme1390I CCNGG 4 cut(s) 374, 1040, 1041, 1734
Bme18I GGWCC 1 cut(s) 85
BmeT110I CYCGRG 1 cut(s) 1039
BmgT120I GGNCC 1 cut(s) 85
BmiI GGNNCC 2 cut(s) 1571, 1855
BmrFI CCNGG 4 cut(s) 374, 1040, 1041, 1734
BmsI GCATC 6 cut(s) 377, 753, 975, 1204, 1655, 1812
BpiI GAAGAC 3 cut(s) 244, 314, 1733
BplI GAGNNNNNCTC 2 cut(s) 1184, 1216
BpmI CTGGAG 1 cut(s) 86
BpuMI CCSGG 3 cut(s) 374, 1040, 1041
BsaBI GATNNNNATC 1 cut(s) 816
BsaI GGTCTC 1 cut(s) 676
BsaJI CCNNGG 3 cut(s) 372, 1039, 1733
BsaWI WCCGGW 2 cut(s) 434, 1315
BsaXI ACNNNNNCTCC 2 cut(s) 604, 634
Bsc4I CCNNNNNNNGG 4 cut(s) 82, 441, 1335, 1356
Bse1I ACTGG 2 cut(s) 1521, 1762
Bse3DI GCAATG 2 cut(s) 723, 1138
Bse8I GATNNNNATC 1 cut(s) 816
BseBI CCWGG 1 cut(s) 1734
BseDI CCNNGG 3 cut(s) 372, 1039, 1733
BseGI GGATG 7 cut(s) 368, 382, 643, 817, 1201, 1219, 1795
BseJI GATNNNNATC 1 cut(s) 816
BseLI CCNNNNNNNGG 4 cut(s) 82, 441, 1335, 1356
BseMI GCAATG 2 cut(s) 723, 1138
BseMII CTCAG 1 cut(s) 1656
BseNI ACTGG 2 cut(s) 1521, 1762
BseRI GAGGAG 1 cut(s) 685
BseYI CCCAGC 2 cut(s) 419, 467
BsgI GTGCAG 3 cut(s) 562, 962, 1759
Bsh1236I CGCG 3 cut(s) 152, 154, 1575
BshFI GGCC 2 cut(s) 171, 1380
BsiHKCI CYCGRG 1 cut(s) 1039
BsiSI CCGG 6 cut(s) 83, 374, 411, 435, 1040, 1316
BslFI GGGAC 1 cut(s) 1691
BslI CCNNNNNNNGG 4 cut(s) 82, 441, 1335, 1356
BsmAI GTCTC 2 cut(s) 676, 868
BsmBI CGTCTC 1 cut(s) 868
BsmFI GGGAC 1 cut(s) 1691
BsnI GGCC 2 cut(s) 171, 1380
Bso31I GGTCTC 1 cut(s) 676
BsoBI CYCGRG 1 cut(s) 1039
Bsp1286I GDGCHC 1 cut(s) 1858
Bsp143I GATC 7 cut(s) 297, 597, 1416, 1453, 1466, 1585, 1746
BspACI CCGC 6 cut(s) 12, 121, 210, 618, 1071, 1573
BspANI GGCC 2 cut(s) 171, 1380
BspCNI CTCAG 1 cut(s) 1655
BspFNI CGCG 3 cut(s) 152, 154, 1575
BspHI TCATGA 1 cut(s) 1114
BspLI GGNNCC 2 cut(s) 1571, 1855
BspMI ACCTGC 2 cut(s) 979, 1347
BspPI GGATC 3 cut(s) 1424, 1461, 1754
BspTNI GGTCTC 1 cut(s) 676
BsrBI CCGCTC 1 cut(s) 123
BsrDI GCAATG 2 cut(s) 723, 1138
BsrI ACTGG 2 cut(s) 1521, 1762
BssECI CCNNGG 3 cut(s) 372, 1039, 1733
BssMI GATC 7 cut(s) 297, 597, 1416, 1453, 1466, 1585, 1746
Bst2UI CCWGG 1 cut(s) 1734
Bst4CI ACNGT 1 cut(s) 700
Bst6I CTCTTC 1 cut(s) 132
BstDEI CTNAG 4 cut(s) 198, 1642, 1840, 1873
BstENI CCTNNNNNAGG 1 cut(s) 1354
BstF5I GGATG 7 cut(s) 368, 382, 643, 817, 1201, 1219, 1795
BstFNI CGCG 3 cut(s) 152, 154, 1575
BstHHI GCGC 1 cut(s) 154
BstKTI GATC 7 cut(s) 300, 600, 1419, 1456, 1469, 1588, 1749
BstMAI GTCTC 2 cut(s) 676, 868
BstMBI GATC 7 cut(s) 297, 597, 1416, 1453, 1466, 1585, 1746
BstMWI GCNNNNNNNGC 1 cut(s) 920
BstNI CCWGG 1 cut(s) 1734
BstNSI RCATGY 2 cut(s) 1058, 1582
BstSCI CCNGG 4 cut(s) 372, 1038, 1039, 1732
BstSFI CTRYAG 3 cut(s) 1351, 1390, 1512
BstUI CGCG 3 cut(s) 152, 154, 1575
BstV2I GAAGAC 3 cut(s) 244, 314, 1733
BstX2I RGATCY 2 cut(s) 1453, 1585
BstXI CCANNNNNNTGG 1 cut(s) 1533
BstYI RGATCY 2 cut(s) 1453, 1585
BsuRI GGCC 2 cut(s) 171, 1380
BtgZI GCGATG 3 cut(s) 63, 175, 938
BtsCI GGATG 7 cut(s) 368, 382, 643, 817, 1201, 1219, 1795
BtsI GCAGTG 1 cut(s) 63
BtsIMutI CAGTG 2 cut(s) 63, 307
BveI ACCTGC 2 cut(s) 979, 1347
CciI TCATGA 1 cut(s) 1114
CfoI GCGC 1 cut(s) 154
Cfr13I GGNCC 1 cut(s) 85
Cfr9I CCCGGG 1 cut(s) 1039
Csp6I GTAC 1 cut(s) 1793
CviAII CATG 9 cut(s) 449, 1055, 1085, 1115, 1256, 1321, 1565, 1579, 1868
CviQI GTAC 1 cut(s) 1793
DdeI CTNAG 4 cut(s) 198, 1642, 1840, 1873
DpnI GATC 7 cut(s) 299, 599, 1418, 1455, 1468, 1587, 1748
DpnII GATC 7 cut(s) 297, 597, 1416, 1453, 1466, 1585, 1746
Eam1104I CTCTTC 1 cut(s) 132
EarI CTCTTC 1 cut(s) 132
EciI GGCGGA 1 cut(s) 199
Eco147I AGGCCT 1 cut(s) 1380
Eco24I GRGCYC 1 cut(s) 1858
Eco31I GGTCTC 1 cut(s) 676
Eco32I GATATC 1 cut(s) 1239
Eco47I GGWCC 1 cut(s) 85
Eco88I CYCGRG 1 cut(s) 1039
EcoNI CCTNNNNNAGG 1 cut(s) 1354
EcoRI GAATTC 1 cut(s) 496
EcoRII CCWGG 1 cut(s) 1732
EcoRV GATATC 1 cut(s) 1239
EcoT22I ATGCAT 1 cut(s) 768
EcoT38I GRGCYC 1 cut(s) 1858
Esp3I CGTCTC 1 cut(s) 868
FaeI CATG 9 cut(s) 452, 1058, 1088, 1118, 1259, 1324, 1568, 1582, 1871
FalI AAGNNNNNCTT 2 cut(s) 236, 268
FaqI GGGAC 1 cut(s) 1691
FatI CATG 9 cut(s) 448, 1054, 1084, 1114, 1255, 1320, 1564, 1578, 1867
FauI CCCGC 1 cut(s) 1064
FbaI TGATCA 1 cut(s) 297
FokI GGATG 7 cut(s) 355, 389, 650, 824, 1208, 1226, 1782
FriOI GRGCYC 1 cut(s) 1858
FspBI CTAG 4 cut(s) 1589, 1613, 1668, 1891
GlaI GCGC 1 cut(s) 153
GsaI CCCAGC 2 cut(s) 423, 471
GsuI CTGGAG 1 cut(s) 86
HaeIII GGCC 2 cut(s) 171, 1380
HapII CCGG 6 cut(s) 83, 374, 411, 435, 1040, 1316
HhaI GCGC 1 cut(s) 154
Hin1II CATG 9 cut(s) 452, 1058, 1088, 1118, 1259, 1324, 1568, 1582, 1871
Hin6I GCGC 1 cut(s) 152
HinP1I GCGC 1 cut(s) 152
HindIII AAGCTT 1 cut(s) 333
HinfI GANTC 7 cut(s) 156, 257, 1111, 1199, 1308, 1765, 1808
HpaII CCGG 6 cut(s) 83, 374, 411, 435, 1040, 1316
HphI GGTGA 6 cut(s) 22, 257, 514, 632, 667, 1003
Hpy166II GTNNAC 2 cut(s) 384, 696
Hpy188I TCNGA 6 cut(s) 47, 314, 481, 602, 811, 958
Hpy188III TCNNGA 8 cut(s) 286, 493, 610, 979, 1115, 1362, 1470, 1668
Hpy8I GTNNAC 2 cut(s) 384, 696
Hpy99I CGWCG 1 cut(s) 692
HpyCH4III ACNGT 1 cut(s) 700
HpyCH4IV ACGT 3 cut(s) 276, 309, 386
HpyF10VI GCNNNNNNNGC 1 cut(s) 920
HpyF3I CTNAG 4 cut(s) 198, 1642, 1840, 1873
HpySE526I ACGT 3 cut(s) 276, 309, 386
Hsp92II CATG 9 cut(s) 452, 1058, 1088, 1118, 1259, 1324, 1568, 1582, 1871
HspAI GCGC 1 cut(s) 152
Ksp22I TGATCA 1 cut(s) 297
Kzo9I GATC 7 cut(s) 297, 597, 1416, 1453, 1466, 1585, 1746
LmnI GCTCC 2 cut(s) 1164, 1853
LweI GCATC 6 cut(s) 377, 753, 975, 1204, 1655, 1812
MaeI CTAG 4 cut(s) 1589, 1613, 1668, 1891
MaeII ACGT 3 cut(s) 276, 309, 386
MaeIII GTNAC 1 cut(s) 1546
MalI GATC 7 cut(s) 299, 599, 1418, 1455, 1468, 1587, 1748
MbiI CCGCTC 1 cut(s) 123
MboI GATC 7 cut(s) 297, 597, 1416, 1453, 1466, 1585, 1746
MboII GAAGA 7 cut(s) 119, 244, 314, 994, 1656, 1683, 1738
MflI RGATCY 2 cut(s) 1453, 1585
MhlI GDGCHC 1 cut(s) 1858
MluCI AATT 9 cut(s) 221, 496, 629, 1080, 1095, 1492, 1651, 1845, 1861
MlyI GAGTC 4 cut(s) 165, 251, 1193, 1759
MmeI TCCRAC 3 cut(s) 627, 771, 1724
Mph1103I ATGCAT 1 cut(s) 768
MroXI GAANNNNTTC 1 cut(s) 551
MseI TTAA 4 cut(s) 1064, 1479, 1490, 1848
MslI CAYNNNNRTG 3 cut(s) 353, 771, 1735
MspI CCGG 6 cut(s) 83, 374, 411, 435, 1040, 1316
MspR9I CCNGG 4 cut(s) 374, 1040, 1041, 1734
MvaI CCWGG 1 cut(s) 1734
MvnI CGCG 3 cut(s) 152, 154, 1575
MwoI GCNNNNNNNGC 1 cut(s) 920
NciI CCSGG 3 cut(s) 374, 1040, 1041
NdeII GATC 7 cut(s) 297, 597, 1416, 1453, 1466, 1585, 1746
NlaIII CATG 9 cut(s) 452, 1058, 1088, 1118, 1259, 1324, 1568, 1582, 1871
NlaIV GGNNCC 2 cut(s) 1571, 1855
NsiI ATGCAT 1 cut(s) 768
NspI RCATGY 2 cut(s) 1058, 1582
OliI CACNNNNGTG 1 cut(s) 1735
PagI TCATGA 1 cut(s) 1114
PaqCI CACCTGC 1 cut(s) 979
PceI AGGCCT 1 cut(s) 1380
PcsI WCGNNNNNNNCGW 1 cut(s) 398
PdmI GAANNNNTTC 1 cut(s) 551
PfeI GAWTC 3 cut(s) 1111, 1308, 1808
PleI GAGTC 4 cut(s) 164, 251, 1193, 1759
PpsI GAGTC 4 cut(s) 164, 251, 1193, 1759
PsiI TTATAA 1 cut(s) 1596
Psp6I CCWGG 1 cut(s) 1732
PspFI CCCAGC 2 cut(s) 419, 467
PspGI CCWGG 1 cut(s) 1732
PspN4I GGNNCC 2 cut(s) 1571, 1855
PspPI GGNCC 1 cut(s) 85
PsuI RGATCY 2 cut(s) 1453, 1585
RsaI GTAC 1 cut(s) 1794
RsaNI GTAC 1 cut(s) 1793
RseI CAYNNNNRTG 3 cut(s) 353, 771, 1735
SaqAI TTAA 4 cut(s) 1064, 1479, 1490, 1848
Sau3AI GATC 7 cut(s) 297, 597, 1416, 1453, 1466, 1585, 1746
Sau96I GGNCC 1 cut(s) 85
SchI GAGTC 4 cut(s) 165, 251, 1193, 1759
ScrFI CCNGG 4 cut(s) 374, 1040, 1041, 1734
SduI GDGCHC 1 cut(s) 1858
SfaNI GCATC 6 cut(s) 377, 753, 975, 1204, 1655, 1812
SfcI CTRYAG 3 cut(s) 1351, 1390, 1512
SinI GGWCC 1 cut(s) 85
SmaI CCCGGG 1 cut(s) 1041
SmiMI CAYNNNNRTG 3 cut(s) 353, 771, 1735
Sse9I AATT 9 cut(s) 221, 496, 629, 1080, 1095, 1492, 1651, 1845, 1861
SseBI AGGCCT 1 cut(s) 1380
SsiI CCGC 6 cut(s) 12, 121, 210, 618, 1071, 1573
SspI AATATT 1 cut(s) 1883
SspMI CTAG 4 cut(s) 1589, 1613, 1668, 1891
StuI AGGCCT 1 cut(s) 1380
StyD4I CCNGG 4 cut(s) 372, 1038, 1039, 1732
TaaI ACNGT 1 cut(s) 700
TaiI ACGT 3 cut(s) 279, 312, 389
TaqI TCGA 6 cut(s) 255, 351, 392, 555, 704, 1465
TasI AATT 9 cut(s) 221, 496, 629, 1080, 1095, 1492, 1651, 1845, 1861
TatI WGTACW 1 cut(s) 1792
TfiI GAWTC 3 cut(s) 1111, 1308, 1808
Tru1I TTAA 4 cut(s) 1064, 1479, 1490, 1848
Tru9I TTAA 4 cut(s) 1064, 1479, 1490, 1848
TscAI CASTG 2 cut(s) 70, 307
TspMI CCCGGG 1 cut(s) 1039
TspRI CASTG 2 cut(s) 70, 307
VpaK11BI GGWCC 1 cut(s) 85
XagI CCTNNNNNAGG 1 cut(s) 1354
XapI RAATTY 5 cut(s) 496, 629, 1080, 1492, 1651
XbaI TCTAGA 1 cut(s) 1667
XceI RCATGY 2 cut(s) 1058, 1582
XmaI CCCGGG 1 cut(s) 1039
XmnI GAANNNNTTC 1 cut(s) 551
XspI CTAG 4 cut(s) 1589, 1613, 1668, 1891
Zsp2I ATGCAT 1 cut(s) 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.