MD13G1224800.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0016816
Physical Location & Seq
Forward (+)
21999021 .. 22000913
1893 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1224800.v1.1.491

Sequence Viewer

Length: 1893 bp
ATGAACCTCTCGTACGCATGCAAGACATTTGCCCTCACAACCTCCCGTTATTACACTTGCGCCACTGCGGTCCACCTTCCCCTGAATCTCCTCCATTTTCTTCAGCTTGCAGGAACCCACCATTCCCTTTCGCTCGCCGAACAAGCTCACGCCCACATTGTCACTCATGGCCTCCAACAAAACCCTTTCCTCGCCACAAAGCTCATCTCTGCTTATGCACTCTGTGGAAAGCCGACCGAGTCGAAACTCGTCTTCGACTCGGCTACTCTCAAGAGTGTGTATTTATGGAACTCACTGATTAATGGCTATGTGAAGAATTGTGCGTACAATGAAGCTTTTGATCTGTTTATGGAAATGTGCAATAGTTATGTATTGCCGGATGGTTATACCTTGGCGACCATGGCGAAAGTTTCAAGCGAGGTTCGGGATTTGGCAACCGGAAAGTATGTTCATGGGAAGGGTTTGAGAGCCGGGTTTGTCGTGGATACCGTTGTTGCGAATTCTCTGATGTCTTTGTATTATAAATGCGGGAGGATTGATGAGTGTCGGAAACTGTTCGATGAAATGCCTCAAAGAAATGTTGGGTCTTGGAATGCGTTAATAGCGGGGTATACAAGTTCTTTGGATCATGATTTTGATAAGGAGATTTGGGAAATTGCTAAATCCATGGTGATGAATGGGGTGAAACCTGACGGATTTACCGTTTCAAGTCTTTTGCCTATGTGTGGAGGGGATGATATCAGGAAATGGGATCATGGGAGAGAACTGCATTGTTATGTTGTGAAGTATGAATTGGATTTCAAGTGGGGTTCGGACGTTCATCTTGGGTGTTGTTTGATTGATATGTATTCTAGGAGTGATAGAGTAGCTTCGGCCAGACGGGTATTTAACCACATGAAGTGTAGAAATGTTTATGCTTGGACGGCGATGGTCAATGGCTATGTGCAGAATGGAGCTCCTGATGAAGCTTTGGTTCTTTTTCAAAAGATGCAGGTGGAAGATGGGGTACAACCCAATAGAGTATCACTTGTGAGTGTCCTCCCTGCTTGTAGTTCGCACGTTGGTTTAATGGGCGGAAAACAAATTCATGGATTTGCTATCAGGAAGGAAATGAGTCGTGAAGTATCTTTGTGTAATGCTTTAATCGATATGTATTCGAAATGTGGGAGCTTGGATTTTGCAAGGCAAGTGTTTGAGGATGATTCTTTTTCTAAGGATGCAATCTCTTGGAGCTCAATGATTTCAGGATATGGATTACATGGAAGGGGTGAGGAAGCCCTTGATGTGTATAATAAGATGCTCCATCTTGGAATCAAACCCGACACGATAACAATCGTTGGGCTCCTTTCTGGTTGTGGAAGGTCAGGATTGGTAAAAGAAGGCCTTTCCATATATAGCTCTGTAACAGCTGATTTTGGAATTAAACCAACGGTGGAGATGTGTGCTTGTGTGGTGGATCTGTTAGGCCGATCAGGAGAGCTTGATCGAGCATTAGATTTCATCAAAACGATGCCTGTAGAACCTGGTCCAAGTGTTTGGGGAGCCTTAGTTACTGCTTCTGTGTTGCATGGGAATCATGACATGCAAGATCTAGCTTATAGATTTCTTATTCAAGTAGAACCCAGAAACCCTTCGAACTATATTTCACTTTCAAATTTTTATGCATTGTCTAGGAGATGGGATGCTGTAGCGGAAATGAGAAGAATGATGAAAGAAAGAGGTTTAAAGAAGGAGCCCGGATGCAGTTGGATAAGCATTAATTCTGAGACTCATTGTTTCTATGTTGCTGATAAATTACATCCTCGTTCAATTTCAATCTATGAGATGCTGGATTACCTCATCTTAGCAATGAAGGGAGCCTGCTCTTCTGAAGCGTTTGAACATCCAGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

631

Amino Acids

70.14

Weight (kDa)

6.62

Isoelectric Point (pI)

31.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 92 - 134 1.1e-09 PPR repeat family
PPR_2 PF13041 162 - 194 4.7e-06 PPR repeat family
PPR PF01535 165 - 194 3.8e-06 PPR repeat
PPR_2 PF13041 302 - 350 2.7e-07 PPR repeat family
PPR PF01535 306 - 332 1.8e-06 PPR repeat
PPR_2 PF13041 406 - 453 2.4e-10 PPR repeat family
PPR PF01535 408 - 438 5.1e-08 PPR repeat
E_motif PF20431 523 - 584 3.8e-17 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016816)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g05100
malus_domestica MD13G1224800.v1.1
prunus_persica Prupe.1G051400_v2.0.a1
pyrus_communis pycom13g19840
rosa_chinensis RchiOBHm_Chr4g0396021
rosa_laevigata RLG00000009580
rosa_multiflora Rmu_sc0030438.1_g000001 Rmu_sc0031838.1_g000001
rosa_roxburghii Rroxscaffold_5G00340810
rosa_rugosa Rorug03G0352900
rosa_samantha Rh4BG063500 Rh4CG070100 Rh4DG060000
rosa_wichuraiana Rw4G005240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 522
AarI CACCTGC 1 cut(s) 982
Acc36I ACCTGC 1 cut(s) 982
AccI GTMKAC 1 cut(s) 611
AciI CCGC 5 cut(s) 68, 528, 605, 1074, 1691
AclWI GGATC 3 cut(s) 633, 759, 1464
AcoI YGGCCR 1 cut(s) 873
AcsI RAATTY 3 cut(s) 499, 1083, 1654
AcuI CTGAAG 2 cut(s) 86, 1890
AdeI CACNNNGTG 1 cut(s) 224
AfaI GTAC 3 cut(s) 14, 326, 1008
AfiI CCNNNNNNNGG 1 cut(s) 725
AgsI TTSAA 9 cut(s) 414, 708, 802, 983, 1613, 1653, 1809, 1815, 1880
AjnI CCWGG 1 cut(s) 1522
AjuI GAANNNNNNNTTGG 2 cut(s) 776, 808
AloI GAACNNNNNNTCC 2 cut(s) 432, 464
Alw21I GWGCWC 2 cut(s) 958, 1235
Alw26I GTCTC 1 cut(s) 1760
AlwI GGATC 3 cut(s) 633, 759, 1464
AoxI GGCC 4 cut(s) 169, 873, 1381, 1465
ApoI RAATTY 3 cut(s) 499, 1083, 1654
AseI ATTAAT 2 cut(s) 300, 1758
Asp700I GAANNNNTTC 2 cut(s) 554, 1630
AspLEI GCGC 1 cut(s) 62
AspS9I GGNCC 2 cut(s) 70, 1526
AsuC2I CCSGG 2 cut(s) 472, 1737
AsuHPI GGTGA 3 cut(s) 682, 694, 1280
AsuII TTCGAA 2 cut(s) 1157, 1634
AvaII GGWCC 2 cut(s) 70, 1526
BaeI ACNNNNGTAYC 2 cut(s) 477, 510
BanII GRGCYC 4 cut(s) 958, 1235, 1344, 1737
BarI GAAGNNNNNNTAC 2 cut(s) 990, 1022
BbsI GAAGAC 1 cut(s) 244
Bbv12I GWGCWC 2 cut(s) 958, 1235
BccI CCATC 5 cut(s) 374, 922, 995, 1311, 1671
BceAI ACGGC 1 cut(s) 939
BciT130I CCWGG 1 cut(s) 1524
BciVI GTATCC 1 cut(s) 478
BcnI CCSGG 2 cut(s) 472, 1737
BcoDI GTCTC 1 cut(s) 1760
BfaI CTAG 3 cut(s) 852, 1592, 1671
BfmI CTRYAG 2 cut(s) 1515, 1686
BfuAI ACCTGC 1 cut(s) 982
BfuI GTATCC 1 cut(s) 478
BglII AGATCT 1 cut(s) 1588
Bme1390I CCNGG 3 cut(s) 472, 1524, 1737
Bme18I GGWCC 2 cut(s) 70, 1526
BmgT120I GGNCC 2 cut(s) 70, 1526
BmiI GGNNCC 5 cut(s) 115, 1343, 1543, 1734, 1858
BmrFI CCNGG 3 cut(s) 472, 1524, 1737
BmsI GCATC 7 cut(s) 978, 1207, 1287, 1500, 1672, 1730, 1815
BpiI GAAGAC 1 cut(s) 244
Bpu14I TTCGAA 2 cut(s) 1157, 1634
BpuEI CTTGAG 1 cut(s) 254
BpuMI CCSGG 2 cut(s) 472, 1737
Bsa29I ATCGAT 1 cut(s) 1146
BsaBI GATNNNNATC 1 cut(s) 1331
BsaJI CCNNGG 3 cut(s) 390, 399, 666
BsaWI WCCGGW 1 cut(s) 437
BsaXI ACNNNNNCTCC 2 cut(s) 1533, 1563
Bsc4I CCNNNNNNNGG 1 cut(s) 725
Bse3DI GCAATG 1 cut(s) 1854
Bse8I GATNNNNATC 1 cut(s) 1331
BseBI CCWGG 1 cut(s) 1524
BseCI ATCGAT 1 cut(s) 1146
BseDI CCNNGG 3 cut(s) 390, 399, 666
BseGI GGATG 8 cut(s) 385, 739, 1204, 1222, 1687, 1745, 1798, 1882
BseJI GATNNNNATC 1 cut(s) 1331
BseLI CCNNNNNNNGG 1 cut(s) 725
BseMI GCAATG 1 cut(s) 1854
BseMII CTCAG 1 cut(s) 1755
BseRI GAGGAG 1 cut(s) 80
BsgI GTGCAG 1 cut(s) 965
Bsh1285I CGRYCG 1 cut(s) 237
BshFI GGCC 4 cut(s) 171, 875, 1383, 1467
BshVI ATCGAT 1 cut(s) 1146
BsiEI CGRYCG 1 cut(s) 237
BsiHKAI GWGCWC 2 cut(s) 958, 1235
BsiSI CCGG 4 cut(s) 377, 438, 471, 1737
BsiWI CGTACG 1 cut(s) 12
BslI CCNNNNNNNGG 1 cut(s) 725
BsmAI GTCTC 1 cut(s) 1760
BsmI GAATGC 1 cut(s) 598
BsnI GGCC 4 cut(s) 171, 875, 1383, 1467
Bsp119I TTCGAA 2 cut(s) 1157, 1634
Bsp1286I GDGCHC 4 cut(s) 958, 1235, 1344, 1737
Bsp143I GATC 7 cut(s) 340, 625, 751, 1456, 1469, 1483, 1588
Bsp19I CCATGG 2 cut(s) 399, 666
BspACI CCGC 5 cut(s) 68, 528, 605, 1074, 1691
BspANI GGCC 4 cut(s) 171, 875, 1383, 1467
BspCNI CTCAG 1 cut(s) 1756
BspDI ATCGAT 1 cut(s) 1146
BspHI TCATGA 2 cut(s) 628, 1576
BspLI GGNNCC 5 cut(s) 115, 1343, 1543, 1734, 1858
BspMI ACCTGC 1 cut(s) 982
BspPI GGATC 3 cut(s) 633, 759, 1464
BspQI GCTCTTC 1 cut(s) 1870
BspT104I TTCGAA 2 cut(s) 1157, 1634
BsrDI GCAATG 1 cut(s) 1854
BssECI CCNNGG 3 cut(s) 390, 399, 666
BssMI GATC 7 cut(s) 340, 625, 751, 1456, 1469, 1483, 1588
BssNAI GTATAC 1 cut(s) 612
BssT1I CCWWGG 3 cut(s) 390, 399, 666
Bst1107I GTATAC 1 cut(s) 612
Bst2UI CCWGG 1 cut(s) 1524
Bst4CI ACNGT 4 cut(s) 490, 555, 703, 1432
Bst6I CTCTTC 1 cut(s) 1870
BstBI TTCGAA 2 cut(s) 1157, 1634
BstC8I GCNNGC 4 cut(s) 19, 108, 135, 1861
BstDEI CTNAG 4 cut(s) 1212, 1546, 1764, 1843
BstDSI CCRYGG 2 cut(s) 399, 666
BstF5I GGATG 8 cut(s) 385, 739, 1204, 1222, 1687, 1745, 1798, 1882
BstHHI GCGC 1 cut(s) 62
BstKTI GATC 7 cut(s) 343, 628, 754, 1459, 1472, 1486, 1591
BstMAI GTCTC 1 cut(s) 1760
BstMBI GATC 7 cut(s) 340, 625, 751, 1456, 1469, 1483, 1588
BstMCI CGRYCG 1 cut(s) 237
BstMWI GCNNNNNNNGC 4 cut(s) 143, 401, 602, 923
BstNI CCWGG 1 cut(s) 1524
BstNSI RCATGY 2 cut(s) 21, 1585
BstSCI CCNGG 3 cut(s) 470, 1522, 1735
BstSFI CTRYAG 2 cut(s) 1515, 1686
BstV2I GAAGAC 1 cut(s) 244
BstX2I RGATCY 2 cut(s) 1456, 1588
BstXI CCANNNNNNTGG 1 cut(s) 1536
BstYI RGATCY 2 cut(s) 1456, 1588
BstZ17I GTATAC 1 cut(s) 612
Bsu15I ATCGAT 1 cut(s) 1146
BsuI GTATCC 1 cut(s) 478
BsuRI GGCC 4 cut(s) 171, 875, 1383, 1467
BsuTUI ATCGAT 1 cut(s) 1146
BtgI CCRYGG 2 cut(s) 399, 666
BtgZI GCGATG 1 cut(s) 941
BtsCI GGATG 8 cut(s) 385, 739, 1204, 1222, 1687, 1745, 1798, 1882
BtsI GCAGTG 1 cut(s) 63
BtsIMutI CAGTG 2 cut(s) 63, 293
BveI ACCTGC 1 cut(s) 982
Cac8I GCNNGC 4 cut(s) 19, 108, 135, 1861
CciI TCATGA 2 cut(s) 628, 1576
CfoI GCGC 1 cut(s) 62
Cfr13I GGNCC 2 cut(s) 70, 1526
ClaI ATCGAT 1 cut(s) 1146
CsiI ACCWGGT 1 cut(s) 1522
Csp6I GTAC 3 cut(s) 13, 325, 1007
CviQI GTAC 3 cut(s) 13, 325, 1007
DdeI CTNAG 4 cut(s) 1212, 1546, 1764, 1843
DpnI GATC 7 cut(s) 342, 627, 753, 1458, 1471, 1485, 1590
DpnII GATC 7 cut(s) 340, 625, 751, 1456, 1469, 1483, 1588
DraI TTTAAA 1 cut(s) 1725
DraIII CACNNNGTG 1 cut(s) 224
EaeI YGGCCR 1 cut(s) 873
Eam1104I CTCTTC 1 cut(s) 1870
EarI CTCTTC 1 cut(s) 1870
EciI GGCGGA 1 cut(s) 1089
Ecl136II GAGCTC 2 cut(s) 956, 1233
Eco130I CCWWGG 3 cut(s) 390, 399, 666
Eco147I AGGCCT 1 cut(s) 1383
Eco24I GRGCYC 4 cut(s) 958, 1235, 1344, 1737
Eco32I GATATC 1 cut(s) 739
Eco47I GGWCC 2 cut(s) 70, 1526
Eco53kI GAGCTC 2 cut(s) 956, 1233
Eco57I CTGAAG 2 cut(s) 86, 1890
EcoICRI GAGCTC 2 cut(s) 956, 1233
EcoRI GAATTC 1 cut(s) 499
EcoRII CCWGG 1 cut(s) 1522
EcoRV GATATC 1 cut(s) 739
EcoT14I CCWWGG 3 cut(s) 390, 399, 666
EcoT22I ATGCAT 1 cut(s) 1666
EcoT38I GRGCYC 4 cut(s) 958, 1235, 1344, 1737
ErhI CCWWGG 3 cut(s) 390, 399, 666
FalI AAGNNNNNCTT 2 cut(s) 1368, 1400
FauI CCCGC 2 cut(s) 521, 598
FblI GTMKAC 1 cut(s) 611
FokI GGATG 8 cut(s) 392, 746, 1211, 1229, 1694, 1752, 1785, 1869
FriOI GRGCYC 4 cut(s) 958, 1235, 1344, 1737
FspBI CTAG 3 cut(s) 852, 1592, 1671
GlaI GCGC 1 cut(s) 61
HaeIII GGCC 4 cut(s) 171, 875, 1383, 1467
HapII CCGG 4 cut(s) 377, 438, 471, 1737
HhaI GCGC 1 cut(s) 62
Hin6I GCGC 1 cut(s) 60
HinP1I GCGC 1 cut(s) 60
HindIII AAGCTT 2 cut(s) 333, 966
HinfI GANTC 8 cut(s) 85, 239, 257, 1114, 1202, 1311, 1573, 1768
HpaII CCGG 4 cut(s) 377, 438, 471, 1737
HphI GGTGA 3 cut(s) 682, 694, 1280
Hpy166II GTNNAC 2 cut(s) 73, 612
Hpy188I TCNGA 5 cut(s) 507, 549, 814, 1765, 1870
Hpy8I GTNNAC 2 cut(s) 73, 612
HpyAV CCTTC 9 cut(s) 86, 451, 1099, 1257, 1353, 1373, 1641, 1723, 1846
HpyCH4III ACNGT 4 cut(s) 490, 555, 703, 1432
HpyCH4IV ACGT 2 cut(s) 816, 1059
HpyF10VI GCNNNNNNNGC 4 cut(s) 143, 401, 602, 923
HpyF3I CTNAG 4 cut(s) 1212, 1546, 1764, 1843
HpySE526I ACGT 2 cut(s) 816, 1059
HspAI GCGC 1 cut(s) 60
Kzo9I GATC 7 cut(s) 340, 625, 751, 1456, 1469, 1483, 1588
LguI GCTCTTC 1 cut(s) 1870
LmnI GCTCC 9 cut(s) 953, 961, 1167, 1230, 1305, 1347, 1541, 1732, 1856
LweI GCATC 7 cut(s) 978, 1207, 1287, 1500, 1672, 1730, 1815
MabI ACCWGGT 1 cut(s) 1522
MaeI CTAG 3 cut(s) 852, 1592, 1671
MaeII ACGT 2 cut(s) 816, 1059
MaeIII GTNAC 3 cut(s) 160, 1402, 1549
MalI GATC 7 cut(s) 342, 627, 753, 1458, 1471, 1485, 1590
MboI GATC 7 cut(s) 340, 625, 751, 1456, 1469, 1483, 1588
MboII GAAGA 6 cut(s) 92, 244, 325, 1010, 1713, 1857
MflI RGATCY 2 cut(s) 1456, 1588
MhlI GDGCHC 4 cut(s) 958, 1235, 1344, 1737
MlyI GAGTC 4 cut(s) 248, 251, 1123, 1762
MmeI TCCRAC 3 cut(s) 199, 527, 1727
Mph1103I ATGCAT 1 cut(s) 1666
MroXI GAANNNNTTC 2 cut(s) 554, 1630
MseI TTAA 8 cut(s) 300, 599, 888, 1067, 1142, 1422, 1724, 1758
MslI CAYNNNNRTG 2 cut(s) 671, 774
MspA1I CMGCKG 1 cut(s) 1409
MspI CCGG 4 cut(s) 377, 438, 471, 1737
MspR9I CCNGG 3 cut(s) 472, 1524, 1737
Mva1269I GAATGC 1 cut(s) 598
MvaI CCWGG 1 cut(s) 1524
MwoI GCNNNNNNNGC 4 cut(s) 143, 401, 602, 923
NciI CCSGG 2 cut(s) 472, 1737
NcoI CCATGG 2 cut(s) 399, 666
NdeII GATC 7 cut(s) 340, 625, 751, 1456, 1469, 1483, 1588
NlaIV GGNNCC 5 cut(s) 115, 1343, 1543, 1734, 1858
NmeAIII GCCGAG 1 cut(s) 239
NmuCI GTSAC 1 cut(s) 160
NsiI ATGCAT 1 cut(s) 1666
NspI RCATGY 2 cut(s) 21, 1585
NspV TTCGAA 2 cut(s) 1157, 1634
PaeI GCATGC 1 cut(s) 21
PagI TCATGA 2 cut(s) 628, 1576
PaqCI CACCTGC 1 cut(s) 982
PceI AGGCCT 1 cut(s) 1383
PciSI GCTCTTC 1 cut(s) 1870
PcsI WCGNNNNNNNCGW 2 cut(s) 486, 699
PctI GAATGC 1 cut(s) 598
PdmI GAANNNNTTC 2 cut(s) 554, 1630
PfeI GAWTC 4 cut(s) 85, 1202, 1311, 1573
Pfl23II CGTACG 1 cut(s) 12
PflFI GACNNNGTC 1 cut(s) 238
PleI GAGTC 4 cut(s) 247, 251, 1122, 1762
PpsI GAGTC 4 cut(s) 247, 251, 1122, 1762
PshBI ATTAAT 2 cut(s) 300, 1758
PsiI TTATAA 1 cut(s) 522
Psp124BI GAGCTC 2 cut(s) 958, 1235
Psp6I CCWGG 1 cut(s) 1522
PspGI CCWGG 1 cut(s) 1522
PspLI CGTACG 1 cut(s) 12
PspN4I GGNNCC 5 cut(s) 115, 1343, 1543, 1734, 1858
PspPI GGNCC 2 cut(s) 70, 1526
PsrI GAACNNNNNNTAC 1 cut(s) 28
PsuI RGATCY 2 cut(s) 1456, 1588
PsyI GACNNNGTC 1 cut(s) 238
PvuII CAGCTG 1 cut(s) 1409
RsaI GTAC 3 cut(s) 14, 326, 1008
RsaNI GTAC 3 cut(s) 13, 325, 1007
RseI CAYNNNNRTG 2 cut(s) 671, 774
SacI GAGCTC 2 cut(s) 958, 1235
SapI GCTCTTC 1 cut(s) 1870
SaqAI TTAA 8 cut(s) 300, 599, 888, 1067, 1142, 1422, 1724, 1758
Sau3AI GATC 7 cut(s) 340, 625, 751, 1456, 1469, 1483, 1588
Sau96I GGNCC 2 cut(s) 70, 1526
SchI GAGTC 4 cut(s) 248, 251, 1123, 1762
ScrFI CCNGG 3 cut(s) 472, 1524, 1737
SduI GDGCHC 4 cut(s) 958, 1235, 1344, 1737
SexAI ACCWGGT 1 cut(s) 1522
SfaNI GCATC 7 cut(s) 978, 1207, 1287, 1500, 1672, 1730, 1815
SfcI CTRYAG 2 cut(s) 1515, 1686
SfuI TTCGAA 2 cut(s) 1157, 1634
SinI GGWCC 2 cut(s) 70, 1526
SmiMI CAYNNNNRTG 2 cut(s) 671, 774
SmlI CTYRAG 1 cut(s) 269
SmoI CTYRAG 1 cut(s) 269
SphI GCATGC 1 cut(s) 21
SseBI AGGCCT 1 cut(s) 1383
SsiI CCGC 5 cut(s) 68, 528, 605, 1074, 1691
SspMI CTAG 3 cut(s) 852, 1592, 1671
SstI GAGCTC 2 cut(s) 958, 1235
StuI AGGCCT 1 cut(s) 1383
StyD4I CCNGG 3 cut(s) 470, 1522, 1735
StyI CCWWGG 3 cut(s) 390, 399, 666
TaaI ACNGT 4 cut(s) 490, 555, 703, 1432
TaiI ACGT 2 cut(s) 819, 1062
TaqI TCGA 7 cut(s) 242, 255, 558, 1146, 1157, 1486, 1634
TaqII GACCGA 1 cut(s) 251
TfiI GAWTC 4 cut(s) 85, 1202, 1311, 1573
Tru1I TTAA 8 cut(s) 300, 599, 888, 1067, 1142, 1422, 1724, 1758
Tru9I TTAA 8 cut(s) 300, 599, 888, 1067, 1142, 1422, 1724, 1758
TscAI CASTG 2 cut(s) 70, 300
TseFI GTSAC 1 cut(s) 160
Tsp45I GTSAC 1 cut(s) 160
TspGWI ACGGA 1 cut(s) 708
TspRI CASTG 2 cut(s) 70, 300
Tth111I GACNNNGTC 1 cut(s) 238
VpaK11BI GGWCC 2 cut(s) 70, 1526
VspI ATTAAT 2 cut(s) 300, 1758
XapI RAATTY 3 cut(s) 499, 1083, 1654
XceI RCATGY 2 cut(s) 21, 1585
XmiI GTMKAC 1 cut(s) 611
XmnI GAANNNNTTC 2 cut(s) 554, 1630
XspI CTAG 3 cut(s) 852, 1592, 1671
Zsp2I ATGCAT 1 cut(s) 1666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.