Prupe.1G051400_v2.0.a1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0016816
Physical Location & Seq
Forward (+)
3604276 .. 3607051
2776 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G051400.1

Sequence Viewer

Length: 1890 bp
ATGAACCTCTCGCCTGCACGCAAGACATTTACCCTCCTAACCTCACGCTATTACACTTGTGCTGTGCTCCACCAGCCTTTGAACCTCCACCATTTTCTTCAGCTTTCAGTTACCCACCACTCTCTTTCACTCACTCAACAAGCTCACGCTCAAGTTCTCACTCATGGCCTCCAACAAAACCCTTTTCTCGCCACAAAGCTCATCTCTGCTTATGCTCTTTGTGGAAACCCAACCAAGTCGAATCTCGTCTTCCACTCTGTTGAGCTCAAGAGTATGTATTTATGGAACTCATTGATTAATGGGTATGTGAGAAATTGTGTTTATAATAAAGCTTTTGAGCTGTTCGACGAAATGTGTAACTGTAATGTATTGCCGGATGATTATACCTTAGCGACCATGGCCAAAGTTTCCGGGGAGGTTCAGGACTTGGTTGCCGGGAAATCTGTTCATGGGAAGAGTATAAGAGTGGGGTTTGTTTTGGATACTGTTGTTGCCAATTCTCTGATGTCTATGTATTATAAATGTGGGAATTTTGGTGAATGTAGGAAAGTATTCGAGGAAATGCCTCAGAGAAACGTGGGGTCTTGGAATGCGATAATAGCAGGATATGCAAGTTCCGTAGATCATGGTTTTGATAAGGAAATTTGGGAAATTGCTAAATGCATGCTGATGAATGGGGTGAAACCTGACGGGTTTAGTGTTTCAAGTCTTTTGAGTTTGTGTGGAGGCGATGATATTGAGAAATGGGATTATGGTAGGGAACTTCACTGTTATATTGTGAAGTATGAATCGGATTTGAAGTGGGGTTCAGATGTTCATCTCGGGTGTTGTTTGATTGATATGTATTCTAGGAGTTACAGAGTTGATTTGGGTAGACGGGTATTTGACCGCATGAAGTGTAGAAATGTATATGCTTGGACGGCCATGATCAATGGTTATGTGCAGAGTGGAGCTCCCGACGAAGCATTGGTTCTTTTGCAAAAGATGCAGGTCGAAGATGGAATACAACCCAATAGAGTATCCCTAGTAAGTGTCCTCCCTGCTTGTAGTTCACATGCTGGTTTAATGTGTGGGAAACAAATTCATGGGTTTGCTATGAGGAAGGAAATGAATCATGATGTATCGTTATGTAATGCTTTGATCGATATGTACTCGAAATGTGGGAGCCTGGATATTGCAAGGCGAGTCTTTGAGGATGATTCTTTTTGTAAGGATGCAATCTCTTGGAGTTCAATGATTTCAGGATATGGTTTACATGGAATAGGTGAGGAAGCCATTGTTTTATATAGTAAGATGCTTCACCTTGGAATCAAACCAGACACAATAACTATAGTTGGGATCCTTTCCGCTTGTGGCAGGTCAGGACTGGTAAACAAAGGCCTTTCCATCTATAGCTCTTTATCAACTGATTTTGGAATTAAACCAACAGTGGAGATCTGTGCTTGTGTGGTGGATCTGTTAGGCCGATCAGGAGACCTCGATGGAGCATTAAATTTCATCAAAACAATGCCTGTAGAACCTGGTCCAAGTGTTTGGGGGTCTTTAGTTACTGCTTCTTTGTTGCATGGGAATCTTGACATGCAAGATCTAGCTTACAGGTTCCTTATTCAACTAGAACCCAAAAACCCGTCAAATTATATTTCGCTATCGAACTTCTATGCTTCTTCTAGGAGGTGGGACGTTGTAGCTGAAATGAGAAGAATGATGAAGGAAAGAGGATTGAAGAAGGCACCTGGGTGCAGCTGGATAAGCATTAATTCAAAGACTCATTGTTTCTATGTTGCTGATAAAGCACATCCTTGTTCTAATTCGATCTATCAGATGCTTGACTATCTTATCTTAGTGATGAAGGGAGCCAGCTATTCTGATGATTTTGAACATCCAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

630

Amino Acids

70.03

Weight (kDa)

7.03

Isoelectric Point (pI)

32.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016816)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g05100
malus_domestica MD13G1224800.v1.1
prunus_persica Prupe.1G051400_v2.0.a1
pyrus_communis pycom13g19840
rosa_chinensis RchiOBHm_Chr4g0396021
rosa_laevigata RLG00000009580
rosa_multiflora Rmu_sc0030438.1_g000001 Rmu_sc0031838.1_g000001
rosa_roxburghii Rroxscaffold_5G00340810
rosa_rugosa Rorug03G0352900
rosa_samantha Rh4BG063500 Rh4CG070100 Rh4DG060000
rosa_wichuraiana Rw4G005240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 324, 519
Acc36I ACCTGC 2 cut(s) 979, 1347
AccB1I GGYRCC 1 cut(s) 1729
AccI GTMKAC 1 cut(s) 874
AciI CCGC 2 cut(s) 889, 1347
AclWI GGATC 3 cut(s) 1333, 1346, 1461
AcoI YGGCCR 2 cut(s) 399, 921
AcsI RAATTY 4 cut(s) 529, 642, 1080, 1492
AcuI CTGAAG 1 cut(s) 83
AfaI GTAC 1 cut(s) 1151
AgsI TTSAA 8 cut(s) 82, 705, 799, 1233, 1610, 1723, 1761, 1877
AjnI CCWGG 3 cut(s) 1167, 1519, 1732
AleI CACNNNNGTG 1 cut(s) 1735
Alw21I GWGCWC 3 cut(s) 69, 267, 955
Alw26I GTCTC 1 cut(s) 1467
AlwI GGATC 3 cut(s) 1333, 1346, 1461
Ama87I CYCGRG 1 cut(s) 821
AoxI GGCC 5 cut(s) 166, 399, 921, 1378, 1462
ApeKI GCWGC 1 cut(s) 1740
ApoI RAATTY 4 cut(s) 529, 642, 1080, 1492
AseI ATTAAT 2 cut(s) 297, 1755
Asp700I GAANNNNTTC 1 cut(s) 551
AspS9I GGNCC 1 cut(s) 1523
AsuC2I CCSGG 2 cut(s) 412, 436
AsuHPI GGTGA 4 cut(s) 548, 691, 1277, 1292
AvaI CYCGRG 1 cut(s) 821
AvaII GGWCC 1 cut(s) 1523
BaeI ACNNNNGTAYC 2 cut(s) 474, 507
BalI TGGCCA 1 cut(s) 401
BamHI GGATCC 1 cut(s) 1338
BanI GGYRCC 1 cut(s) 1729
BanII GRGCYC 2 cut(s) 267, 955
BarI GAAGNNNNNNTAC 2 cut(s) 987, 1019
BbsI GAAGAC 1 cut(s) 241
Bbv12I GWGCWC 3 cut(s) 69, 267, 955
BbvI GCAGC 1 cut(s) 1752
BccI CCATC 3 cut(s) 992, 1394, 1475
BceAI ACGGC 1 cut(s) 936
BciT130I CCWGG 3 cut(s) 1169, 1521, 1734
BciVI GTATCC 2 cut(s) 475, 1030
BclI TGATCA 1 cut(s) 927
BcnI CCSGG 2 cut(s) 412, 436
BcoDI GTCTC 1 cut(s) 1467
BfaI CTAG 5 cut(s) 849, 1025, 1589, 1613, 1668
BfmI CTRYAG 3 cut(s) 1329, 1390, 1512
BfuAI ACCTGC 2 cut(s) 979, 1347
BfuI GTATCC 2 cut(s) 475, 1030
BglII AGATCT 2 cut(s) 1434, 1585
BisI GCNGC 1 cut(s) 1741
BlsI GCNGC 1 cut(s) 1742
Bme1390I CCNGG 5 cut(s) 412, 436, 1169, 1521, 1734
Bme18I GGWCC 1 cut(s) 1523
BmeT110I CYCGRG 1 cut(s) 821
BmgT120I GGNCC 1 cut(s) 1523
BmiI GGNNCC 5 cut(s) 1166, 1340, 1601, 1731, 1855
BmrFI CCNGG 5 cut(s) 412, 436, 1169, 1521, 1734
BmsI GCATC 4 cut(s) 975, 1204, 1284, 1812
BpiI GAAGAC 1 cut(s) 241
BplI GAGNNNNNCTC 2 cut(s) 937, 969
Bpu10I CCTNAGC 1 cut(s) 388
BpuEI CTTGAG 2 cut(s) 135, 251
BpuMI CCSGG 2 cut(s) 412, 436
Bsa29I ATCGAT 1 cut(s) 1143
BsaBI GATNNNNATC 1 cut(s) 816
BsaI GGTCTC 1 cut(s) 1467
BsaJI CCNNGG 4 cut(s) 396, 411, 1303, 1733
Bse1I ACTGG 1 cut(s) 1371
Bse8I GATNNNNATC 1 cut(s) 816
BseBI CCWGG 3 cut(s) 1169, 1521, 1734
BseCI ATCGAT 1 cut(s) 1143
BseDI CCNNGG 4 cut(s) 396, 411, 1303, 1733
BseGI GGATG 5 cut(s) 382, 1201, 1219, 1795, 1879
BseJI GATNNNNATC 1 cut(s) 816
BseMII CTCAG 1 cut(s) 581
BseNI ACTGG 1 cut(s) 1371
BseXI GCAGC 1 cut(s) 1752
BsgI GTGCAG 2 cut(s) 962, 1759
BshFI GGCC 5 cut(s) 168, 401, 923, 1380, 1464
BshNI GGYRCC 1 cut(s) 1729
BshVI ATCGAT 1 cut(s) 1143
BsiHKAI GWGCWC 3 cut(s) 69, 267, 955
BsiHKCI CYCGRG 1 cut(s) 821
BsiSI CCGG 3 cut(s) 374, 411, 435
BslFI GGGAC 1 cut(s) 1691
BsmAI GTCTC 1 cut(s) 1467
BsmFI GGGAC 1 cut(s) 1691
BsmI GAATGC 1 cut(s) 595
BsnI GGCC 5 cut(s) 168, 401, 923, 1380, 1464
Bso31I GGTCTC 1 cut(s) 1467
BsoBI CYCGRG 1 cut(s) 821
Bsp1286I GDGCHC 3 cut(s) 69, 267, 955
Bsp143I GATC 9 cut(s) 622, 927, 1140, 1338, 1434, 1453, 1466, 1585, 1812
Bsp19I CCATGG 1 cut(s) 396
BspACI CCGC 2 cut(s) 889, 1347
BspANI GGCC 5 cut(s) 168, 401, 923, 1380, 1464
BspCNI CTCAG 1 cut(s) 580
BspDI ATCGAT 1 cut(s) 1143
BspHI TCATGA 1 cut(s) 1114
BspLI GGNNCC 5 cut(s) 1166, 1340, 1601, 1731, 1855
BspMI ACCTGC 2 cut(s) 979, 1347
BspPI GGATC 3 cut(s) 1333, 1346, 1461
BspT107I GGYRCC 1 cut(s) 1729
BspTNI GGTCTC 1 cut(s) 1467
BsrI ACTGG 1 cut(s) 1371
BssECI CCNNGG 4 cut(s) 396, 411, 1303, 1733
BssMI GATC 9 cut(s) 622, 927, 1140, 1338, 1434, 1453, 1466, 1585, 1812
BssT1I CCWWGG 2 cut(s) 396, 1303
Bst2UI CCWGG 3 cut(s) 1169, 1521, 1734
Bst4CI ACNGT 4 cut(s) 362, 487, 770, 1429
Bst6I CTCTTC 1 cut(s) 449
BstAPI GCANNNNNTGC 2 cut(s) 608, 985
BstC8I GCNNGC 4 cut(s) 15, 19, 665, 1858
BstDEI CTNAG 3 cut(s) 388, 567, 1840
BstDSI CCRYGG 1 cut(s) 396
BstF5I GGATG 5 cut(s) 382, 1201, 1219, 1795, 1879
BstKTI GATC 9 cut(s) 625, 930, 1143, 1341, 1437, 1456, 1469, 1588, 1815
BstMAI GTCTC 1 cut(s) 1467
BstMBI GATC 9 cut(s) 622, 927, 1140, 1338, 1434, 1453, 1466, 1585, 1812
BstMWI GCNNNNNNNGC 8 cut(s) 73, 398, 599, 608, 920, 985, 1749, 1790
BstNI CCWGG 3 cut(s) 1169, 1521, 1734
BstNSI RCATGY 3 cut(s) 667, 1058, 1582
BstSCI CCNGG 5 cut(s) 410, 434, 1167, 1519, 1732
BstSFI CTRYAG 3 cut(s) 1329, 1390, 1512
BstV1I GCAGC 1 cut(s) 1752
BstV2I GAAGAC 1 cut(s) 241
BstX2I RGATCY 4 cut(s) 1338, 1434, 1453, 1585
BstXI CCANNNNNNTGG 1 cut(s) 1533
BstYI RGATCY 4 cut(s) 1338, 1434, 1453, 1585
Bsu15I ATCGAT 1 cut(s) 1143
BsuI GTATCC 2 cut(s) 475, 1030
BsuRI GGCC 5 cut(s) 168, 401, 923, 1380, 1464
BsuTUI ATCGAT 1 cut(s) 1143
BtgI CCRYGG 1 cut(s) 396
BtgZI GCGATG 1 cut(s) 744
BtsCI GGATG 5 cut(s) 382, 1201, 1219, 1795, 1879
BtsIMutI CAGTG 2 cut(s) 766, 1434
BveI ACCTGC 2 cut(s) 979, 1347
Cac8I GCNNGC 4 cut(s) 15, 19, 665, 1858
CciI TCATGA 1 cut(s) 1114
Cfr13I GGNCC 1 cut(s) 1523
ClaI ATCGAT 1 cut(s) 1143
CsiI ACCWGGT 1 cut(s) 1519
Csp6I GTAC 1 cut(s) 1150
CviQI GTAC 1 cut(s) 1150
DdeI CTNAG 3 cut(s) 388, 567, 1840
DpnI GATC 9 cut(s) 624, 929, 1142, 1340, 1436, 1455, 1468, 1587, 1814
DpnII GATC 9 cut(s) 622, 927, 1140, 1338, 1434, 1453, 1466, 1585, 1812
EaeI YGGCCR 2 cut(s) 399, 921
Eam1104I CTCTTC 1 cut(s) 449
EarI CTCTTC 1 cut(s) 449
Ecl136II GAGCTC 2 cut(s) 265, 953
Eco130I CCWWGG 2 cut(s) 396, 1303
Eco147I AGGCCT 1 cut(s) 1380
Eco24I GRGCYC 2 cut(s) 267, 955
Eco31I GGTCTC 1 cut(s) 1467
Eco47I GGWCC 1 cut(s) 1523
Eco53kI GAGCTC 2 cut(s) 265, 953
Eco57I CTGAAG 1 cut(s) 83
Eco88I CYCGRG 1 cut(s) 821
EcoICRI GAGCTC 2 cut(s) 265, 953
EcoRII CCWGG 3 cut(s) 1167, 1519, 1732
EcoT14I CCWWGG 2 cut(s) 396, 1303
EcoT22I ATGCAT 1 cut(s) 665
EcoT38I GRGCYC 2 cut(s) 267, 955
ErhI CCWWGG 2 cut(s) 396, 1303
FaqI GGGAC 1 cut(s) 1691
FbaI TGATCA 1 cut(s) 927
FblI GTMKAC 1 cut(s) 874
Fnu4HI GCNGC 1 cut(s) 1741
FokI GGATG 5 cut(s) 389, 1208, 1226, 1782, 1866
FriOI GRGCYC 2 cut(s) 267, 955
Fsp4HI GCNGC 1 cut(s) 1741
FspBI CTAG 5 cut(s) 849, 1025, 1589, 1613, 1668
GluI GCNGC 1 cut(s) 1741
HaeIII GGCC 5 cut(s) 168, 401, 923, 1380, 1464
HapII CCGG 3 cut(s) 374, 411, 435
HindIII AAGCTT 1 cut(s) 330
HinfI GANTC 8 cut(s) 241, 788, 1111, 1185, 1199, 1308, 1570, 1765
HpaII CCGG 3 cut(s) 374, 411, 435
HphI GGTGA 4 cut(s) 548, 691, 1277, 1292
Hpy166II GTNNAC 4 cut(s) 875, 1052, 1253, 1372
Hpy188I TCNGA 6 cut(s) 504, 570, 793, 811, 1821, 1867
Hpy188III TCNNGA 8 cut(s) 268, 422, 956, 1115, 1242, 1362, 1470, 1574
Hpy8I GTNNAC 4 cut(s) 875, 1052, 1253, 1372
Hpy99I CGWCG 2 cut(s) 350, 962
HpyAV CCTTC 4 cut(s) 1096, 1702, 1720, 1843
HpyCH4III ACNGT 4 cut(s) 362, 487, 770, 1429
HpyCH4IV ACGT 2 cut(s) 576, 1680
HpyF10VI GCNNNNNNNGC 8 cut(s) 73, 398, 599, 608, 920, 985, 1749, 1790
HpyF3I CTNAG 3 cut(s) 388, 567, 1840
HpySE526I ACGT 2 cut(s) 576, 1680
Ksp22I TGATCA 1 cut(s) 927
Kzo9I GATC 9 cut(s) 622, 927, 1140, 1338, 1434, 1453, 1466, 1585, 1812
LmnI GCTCC 6 cut(s) 72, 950, 958, 1164, 1484, 1853
Lsp1109I GCAGC 1 cut(s) 1752
LweI GCATC 4 cut(s) 975, 1204, 1284, 1812
MabI ACCWGGT 1 cut(s) 1519
MaeI CTAG 5 cut(s) 849, 1025, 1589, 1613, 1668
MaeII ACGT 2 cut(s) 576, 1680
MaeIII GTNAC 4 cut(s) 109, 356, 854, 1546
MalI GATC 9 cut(s) 624, 929, 1142, 1340, 1436, 1455, 1468, 1587, 1814
MboI GATC 9 cut(s) 622, 927, 1140, 1338, 1434, 1453, 1466, 1585, 1812
MboII GAAGA 7 cut(s) 89, 241, 466, 1007, 1656, 1710, 1735
MflI RGATCY 4 cut(s) 1338, 1434, 1453, 1585
MhlI GDGCHC 3 cut(s) 69, 267, 955
MlsI TGGCCA 1 cut(s) 401
MluNI TGGCCA 1 cut(s) 401
MlyI GAGTC 2 cut(s) 1194, 1759
MmeI TCCRAC 1 cut(s) 196
Mox20I TGGCCA 1 cut(s) 401
Mph1103I ATGCAT 1 cut(s) 665
MroXI GAANNNNTTC 1 cut(s) 551
MscI TGGCCA 1 cut(s) 401
MseI TTAA 5 cut(s) 297, 1064, 1419, 1490, 1755
MslI CAYNNNNRTG 2 cut(s) 668, 1735
Msp20I TGGCCA 1 cut(s) 401
MspA1I CMGCKG 1 cut(s) 1743
MspI CCGG 3 cut(s) 374, 411, 435
MspR9I CCNGG 5 cut(s) 412, 436, 1169, 1521, 1734
Mva1269I GAATGC 1 cut(s) 595
MvaI CCWGG 3 cut(s) 1169, 1521, 1734
MwoI GCNNNNNNNGC 8 cut(s) 73, 398, 599, 608, 920, 985, 1749, 1790
NciI CCSGG 2 cut(s) 412, 436
NcoI CCATGG 1 cut(s) 396
NdeII GATC 9 cut(s) 622, 927, 1140, 1338, 1434, 1453, 1466, 1585, 1812
NlaIV GGNNCC 5 cut(s) 1166, 1340, 1601, 1731, 1855
NsiI ATGCAT 1 cut(s) 665
NspI RCATGY 3 cut(s) 667, 1058, 1582
OliI CACNNNNGTG 1 cut(s) 1735
PaeI GCATGC 1 cut(s) 667
PagI TCATGA 1 cut(s) 1114
PceI AGGCCT 1 cut(s) 1380
PctI GAATGC 1 cut(s) 595
PdmI GAANNNNTTC 1 cut(s) 551
PfeI GAWTC 6 cut(s) 241, 788, 1111, 1199, 1308, 1570
PkrI GCNGC 1 cut(s) 1742
PleI GAGTC 2 cut(s) 1193, 1759
PpsI GAGTC 2 cut(s) 1193, 1759
PshBI ATTAAT 2 cut(s) 297, 1755
PsiI TTATAA 2 cut(s) 324, 519
Psp124BI GAGCTC 2 cut(s) 267, 955
Psp6I CCWGG 3 cut(s) 1167, 1519, 1732
PspGI CCWGG 3 cut(s) 1167, 1519, 1732
PspN4I GGNNCC 5 cut(s) 1166, 1340, 1601, 1731, 1855
PspPI GGNCC 1 cut(s) 1523
PsuI RGATCY 4 cut(s) 1338, 1434, 1453, 1585
PvuII CAGCTG 1 cut(s) 1743
RsaI GTAC 1 cut(s) 1151
RsaNI GTAC 1 cut(s) 1150
RseI CAYNNNNRTG 2 cut(s) 668, 1735
SacI GAGCTC 2 cut(s) 267, 955
SaqAI TTAA 5 cut(s) 297, 1064, 1419, 1490, 1755
SatI GCNGC 1 cut(s) 1741
Sau3AI GATC 9 cut(s) 622, 927, 1140, 1338, 1434, 1453, 1466, 1585, 1812
Sau96I GGNCC 1 cut(s) 1523
SchI GAGTC 2 cut(s) 1194, 1759
ScrFI CCNGG 5 cut(s) 412, 436, 1169, 1521, 1734
SduI GDGCHC 3 cut(s) 69, 267, 955
SexAI ACCWGGT 1 cut(s) 1519
SfaNI GCATC 4 cut(s) 975, 1204, 1284, 1812
SfcI CTRYAG 3 cut(s) 1329, 1390, 1512
SinI GGWCC 1 cut(s) 1523
SmiMI CAYNNNNRTG 2 cut(s) 668, 1735
SmlI CTYRAG 2 cut(s) 150, 266
SmoI CTYRAG 2 cut(s) 150, 266
SphI GCATGC 1 cut(s) 667
SseBI AGGCCT 1 cut(s) 1380
SsiI CCGC 2 cut(s) 889, 1347
SspMI CTAG 5 cut(s) 849, 1025, 1589, 1613, 1668
SstI GAGCTC 2 cut(s) 267, 955
StuI AGGCCT 1 cut(s) 1380
StyD4I CCNGG 5 cut(s) 410, 434, 1167, 1519, 1732
StyI CCWWGG 2 cut(s) 396, 1303
TaaI ACNGT 4 cut(s) 362, 487, 770, 1429
TaiI ACGT 2 cut(s) 579, 1683
TaqI TCGA 9 cut(s) 239, 345, 555, 993, 1143, 1154, 1479, 1649, 1811
TatI WGTACW 1 cut(s) 1149
TfiI GAWTC 6 cut(s) 241, 788, 1111, 1199, 1308, 1570
Tru1I TTAA 5 cut(s) 297, 1064, 1419, 1490, 1755
Tru9I TTAA 5 cut(s) 297, 1064, 1419, 1490, 1755
TscAI CASTG 2 cut(s) 773, 1434
TseI GCWGC 1 cut(s) 1740
TspGWI ACGGA 1 cut(s) 607
TspRI CASTG 2 cut(s) 773, 1434
VpaK11BI GGWCC 1 cut(s) 1523
VspI ATTAAT 2 cut(s) 297, 1755
XapI RAATTY 4 cut(s) 529, 642, 1080, 1492
XceI RCATGY 3 cut(s) 667, 1058, 1582
XmiI GTMKAC 1 cut(s) 874
XmnI GAANNNNTTC 1 cut(s) 551
XspI CTAG 5 cut(s) 849, 1025, 1589, 1613, 1668
Zsp2I ATGCAT 1 cut(s) 665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.