FvH4_4g06280

F-box domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014709
Physical Location & Seq
Reverse (-)
5594617 .. 5597278
2662 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g06280.t2

Sequence Viewer

Length: 1266 bp
ATGTCAGTCTCATCATCAGCCACAGATGTTGCCAACAACGAAGATCTTCTATCGCAAATTCTTGTTCGATTACCCCCAAAATCCCTCCTGTGCTTCAAATCTGTGTCCAAGCAGTGGCTTTCTCTCATTTCCAGCCCCCAATTCATTGTCACCCACATCCTCTGCCGAGGCAGCGCATCCCCTTCTGGCCTACTCCTAAACAATGACTACTTGCATACTCCGGAGTTCCACTATGTTCCTCTGACACACTCTAATCCCTCTCAAACTTCCCCTCCATATCTGAGCTATCTTGGCGCCCCACGCATCAAGATCCTACAGTCTTGTAATGGTTTGCTCTTGTGTTCTTCTGCCTACCAAAAAAATGATCCAAATTATGAACTCAAATACTTTGATGACTTCACGCAGACAGAGGATGATGTTCTCTTGCCAGACATATGGCATGTTATCCCAACAATCTCAAACTCCTGCATTCAAACAAGAATCCATTATTCTGGTTTTAGATTCTATGTTTGCAATCCCACCACCAAGCAGTTCAATACCCTTTCTTTTCCTTTCTTTCAGTACCACATACTTGCTCTTAATCTGGCTTTTGATCCTTTGCATTCACCCTACTACAAAATATTGTGTATACTCAAGAAGAATCTGGATACTTTTATAATTCACCTGTATAATTCCGAGACAAAAACTTGGAACCCATCTTCGGTATCTTTTAATGCACCCTGGGAAATATCCTTCCGTGAACCTGTGTTTTGTAGGCGTGCAATTCACTGGTATAGTGACCGGCAGACTTCTCTTTACTATGATGTGGATAAGGAGTGCTTGAACCCAATGCCTATGCCCCCGTCTTCGACTTATGGAACTGTCAATTATTTTGGGGAGTCTGGAGGCCATCTGCATCTGATAATGTCCTATGTGCTAGAACTCGATGTGTTTGAGTTACAGAAAGATTATTCTGCATGGTCAGTGAGGTATCATATCAATCTTGAAGCCATAAGAGTTTCACTCTATGGTATGTGTGGCTTTTCCAGCAACGAATTCATTGCTTCAGTACTGAGAATTACTCGTGGTGAAAAGGAAGAGGAATCAGAGGCGGTGGTATTTATGTTTGGTGTGGAAGGTATTATGGTTGTCTCTTACAATTTGAGTGATGGGACACATAGAAGACATACAGGTTTGGTTCCTTCTCGGGGAGATGTTCATGATTTCAAGCTGCTCCATTCCTATAGAGACACCTTTCCATTCATAGAGACTCTGTCTCCTATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

422

Amino Acids

48.25

Weight (kDa)

6.08

Isoelectric Point (pI)

54.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 14 - 49 2.5e-07 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g06280 FvH4_4g06280 FvH4_4g06280 FvH4_4g06280
malus_domestica MD16G1239800.v1.1
rosa_chinensis RchiOBHm_Chr4g0398001
rosa_laevigata RLG00000009391
rosa_multiflora Rmu_sc0003773.1_g000024
rosa_roxburghii Rroxscaffold_5G00342960
rosa_rugosa Rorug04G0005200 Rorug04G0005300
rosa_samantha Rh4AG081900 Rh4BG079600 Rh4CG089400 Rh4DG075000
rosa_wichuraiana Rw0G019320 Rw4G006830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 656
AccB1I GGYRCC 1 cut(s) 293
AccB7I CCANNNNNTGG 1 cut(s) 114
AccI GTMKAC 1 cut(s) 628
AccIII TCCGGA 1 cut(s) 220
AciI CCGC 1 cut(s) 1091
AclWI GGATC 3 cut(s) 304, 359, 587
AcsI RAATTY 2 cut(s) 57, 1034
AcuI CTGAAG 1 cut(s) 1029
AcyI GRCGYC 1 cut(s) 294
AfaI GTAC 2 cut(s) 563, 1050
AfiI CCNNNNNNNGG 3 cut(s) 114, 700, 1187
AgsI TTSAA 6 cut(s) 97, 473, 535, 823, 986, 1207
AjnI CCWGG 1 cut(s) 719
AluBI AGCT 2 cut(s) 285, 1210
AluI AGCT 2 cut(s) 285, 1210
Alw26I GTCTC 6 cut(s) 13, 671, 1135, 1221, 1241, 1260
AlwI GGATC 3 cut(s) 304, 359, 587
Ama87I CYCGRG 1 cut(s) 1185
Aor13HI TCCGGA 1 cut(s) 220
AoxI GGCC 2 cut(s) 187, 886
ApeKI GCWGC 2 cut(s) 171, 1210
ApoI RAATTY 2 cut(s) 57, 1034
Asp700I GAANNNNTTC 1 cut(s) 45
AspLEI GCGC 2 cut(s) 176, 296
AsuHPI GGTGA 4 cut(s) 142, 597, 653, 1079
AvaI CYCGRG 1 cut(s) 1185
BanI GGYRCC 1 cut(s) 293
BauI CACGAG 1 cut(s) 1062
BbsI GAAGAC 2 cut(s) 837, 1168
BbvI GCAGC 2 cut(s) 183, 1197
BccI CCATC 3 cut(s) 703, 897, 1142
BcgI CGANNNNNNTGC 2 cut(s) 1022, 1056
BciT130I CCWGG 1 cut(s) 721
BciVI GTATCC 1 cut(s) 640
BcoDI GTCTC 6 cut(s) 13, 671, 1135, 1221, 1241, 1260
BfaI CTAG 1 cut(s) 917
BfmI CTRYAG 2 cut(s) 314, 1222
BfoI RGCGCY 1 cut(s) 297
BfuI GTATCC 1 cut(s) 640
BglII AGATCT 1 cut(s) 43
BisI GCNGC 2 cut(s) 172, 1211
BlsI GCNGC 2 cut(s) 173, 1212
BmcAI AGTACT 1 cut(s) 1050
Bme1390I CCNGG 1 cut(s) 721
BmeT110I CYCGRG 1 cut(s) 1185
BmiI GGNNCC 3 cut(s) 295, 692, 1179
BmrFI CCNGG 1 cut(s) 721
BmsI GCATC 3 cut(s) 185, 312, 904
BpiI GAAGAC 2 cut(s) 837, 1168
BplI GAGNNNNNCTC 4 cut(s) 987, 1019, 1045, 1077
BpmI CTGGAG 1 cut(s) 903
BpuEI CTTGAG 1 cut(s) 617
BsaHI GRCGYC 1 cut(s) 294
BsaJI CCNNGG 3 cut(s) 166, 719, 720
BsaWI WCCGGW 1 cut(s) 220
BsaXI ACNNNNNCTCC 3 cut(s) 256, 286, 1240
Bsc4I CCNNNNNNNGG 3 cut(s) 114, 700, 1187
Bse118I RCCGGY 1 cut(s) 780
Bse1I ACTGG 1 cut(s) 773
Bse3DI GCAATG 1 cut(s) 1038
BseAI TCCGGA 1 cut(s) 220
BseBI CCWGG 1 cut(s) 721
BseDI CCNNGG 3 cut(s) 166, 719, 720
BseGI GGATG 3 cut(s) 156, 176, 418
BseLI CCNNNNNNNGG 3 cut(s) 114, 700, 1187
BseMI GCAATG 1 cut(s) 1038
BseMII CTCAG 2 cut(s) 272, 1043
BseNI ACTGG 1 cut(s) 773
BseXI GCAGC 2 cut(s) 183, 1197
BshFI GGCC 2 cut(s) 189, 888
BshNI GGYRCC 1 cut(s) 293
BsiHKCI CYCGRG 1 cut(s) 1185
BsiSI CCGG 2 cut(s) 221, 781
BslFI GGGAC 1 cut(s) 1165
BslI CCNNNNNNNGG 3 cut(s) 114, 700, 1187
BsmAI GTCTC 6 cut(s) 13, 671, 1135, 1221, 1241, 1260
BsmFI GGGAC 1 cut(s) 1165
BsmI GAATGC 2 cut(s) 468, 601
BsnI GGCC 2 cut(s) 189, 888
BsoBI CYCGRG 1 cut(s) 1185
Bsp13I TCCGGA 1 cut(s) 220
Bsp143I GATC 4 cut(s) 43, 309, 364, 592
BspACI CCGC 1 cut(s) 1091
BspANI GGCC 2 cut(s) 189, 888
BspCNI CTCAG 2 cut(s) 273, 1044
BspEI TCCGGA 1 cut(s) 220
BspHI TCATGA 1 cut(s) 1198
BspLI GGNNCC 3 cut(s) 295, 692, 1179
BspPI GGATC 3 cut(s) 304, 359, 587
BspT107I GGYRCC 1 cut(s) 293
BsrDI GCAATG 1 cut(s) 1038
BsrFI RCCGGY 1 cut(s) 780
BsrI ACTGG 1 cut(s) 773
BssAI RCCGGY 1 cut(s) 780
BssECI CCNNGG 3 cut(s) 166, 719, 720
BssMI GATC 4 cut(s) 43, 309, 364, 592
BssNAI GTATAC 1 cut(s) 629
BssNI GRCGYC 1 cut(s) 294
BssSI CACGAG 1 cut(s) 1062
Bst1107I GTATAC 1 cut(s) 629
Bst2BI CACGAG 1 cut(s) 1062
Bst2UI CCWGG 1 cut(s) 721
Bst4CI ACNGT 2 cut(s) 318, 862
Bst6I CTCTTC 1 cut(s) 1071
BstACI GRCGYC 1 cut(s) 294
BstC8I GCNNGC 1 cut(s) 759
BstDEI CTNAG 2 cut(s) 281, 1052
BstF5I GGATG 3 cut(s) 156, 176, 418
BstH2I RGCGCY 1 cut(s) 297
BstHHI GCGC 2 cut(s) 176, 296
BstKTI GATC 4 cut(s) 46, 312, 367, 595
BstMAI GTCTC 6 cut(s) 13, 671, 1135, 1221, 1241, 1260
BstMBI GATC 4 cut(s) 43, 309, 364, 592
BstMWI GCNNNNNNNGC 4 cut(s) 171, 291, 300, 1026
BstNI CCWGG 1 cut(s) 721
BstNSI RCATGY 1 cut(s) 443
BstSCI CCNGG 1 cut(s) 719
BstSFI CTRYAG 2 cut(s) 314, 1222
BstV1I GCAGC 2 cut(s) 183, 1197
BstV2I GAAGAC 2 cut(s) 837, 1168
BstX2I RGATCY 2 cut(s) 43, 309
BstXI CCANNNNNNTGG 2 cut(s) 435, 491
BstYI RGATCY 2 cut(s) 43, 309
BstZ17I GTATAC 1 cut(s) 629
BsuI GTATCC 1 cut(s) 640
BsuRI GGCC 2 cut(s) 189, 888
BtsCI GGATG 3 cut(s) 156, 176, 418
BtsI GCAGTG 1 cut(s) 119
BtsIMutI CAGTG 3 cut(s) 119, 766, 969
Cac8I GCNNGC 1 cut(s) 759
CciI TCATGA 1 cut(s) 1198
CfoI GCGC 2 cut(s) 176, 296
Cfr10I RCCGGY 1 cut(s) 780
Csp6I GTAC 2 cut(s) 562, 1049
CspCI CAANNNNNGTGG 2 cut(s) 10, 45
CviAII CATG 3 cut(s) 440, 957, 1199
CviQI GTAC 2 cut(s) 562, 1049
DdeI CTNAG 2 cut(s) 281, 1052
DinI GGCGCC 1 cut(s) 295
DpnI GATC 4 cut(s) 45, 311, 366, 594
DpnII GATC 4 cut(s) 43, 309, 364, 592
Eam1104I CTCTTC 1 cut(s) 1071
EarI CTCTTC 1 cut(s) 1071
Eco57I CTGAAG 1 cut(s) 1029
Eco88I CYCGRG 1 cut(s) 1185
EcoRI GAATTC 1 cut(s) 1034
EcoRII CCWGG 1 cut(s) 719
EgeI GGCGCC 1 cut(s) 295
EheI GGCGCC 1 cut(s) 295
FaeI CATG 3 cut(s) 443, 960, 1202
FalI AAGNNNNNCTT 2 cut(s) 803, 835
FaqI GGGAC 1 cut(s) 1165
FatI CATG 3 cut(s) 439, 956, 1198
FauNDI CATATG 1 cut(s) 434
FblI GTMKAC 1 cut(s) 628
Fnu4HI GCNGC 2 cut(s) 172, 1211
FokI GGATG 3 cut(s) 143, 163, 425
Fsp4HI GCNGC 2 cut(s) 172, 1211
FspBI CTAG 1 cut(s) 917
GlaI GCGC 2 cut(s) 175, 295
GluI GCNGC 2 cut(s) 172, 1211
GsuI CTGGAG 1 cut(s) 903
HaeII RGCGCY 1 cut(s) 297
HaeIII GGCC 2 cut(s) 189, 888
HapII CCGG 2 cut(s) 221, 781
HhaI GCGC 2 cut(s) 176, 296
Hin1I GRCGYC 1 cut(s) 294
Hin1II CATG 3 cut(s) 443, 960, 1202
Hin6I GCGC 2 cut(s) 174, 294
HinP1I GCGC 2 cut(s) 174, 294
HinfI GANTC 6 cut(s) 480, 501, 640, 878, 1082, 1249
HpaII CCGG 2 cut(s) 221, 781
HphI GGTGA 4 cut(s) 142, 597, 653, 1079
Hpy166II GTNNAC 2 cut(s) 629, 740
Hpy188I TCNGA 5 cut(s) 243, 282, 676, 900, 1087
Hpy188III TCNNGA 7 cut(s) 221, 307, 634, 644, 882, 983, 1199
Hpy8I GTNNAC 2 cut(s) 629, 740
HpyAV CCTTC 4 cut(s) 192, 742, 1109, 1191
HpyCH4III ACNGT 2 cut(s) 318, 862
HpyCH4V TGCA 8 cut(s) 214, 468, 513, 601, 716, 761, 895, 956
HpyF10VI GCNNNNNNNGC 4 cut(s) 171, 291, 300, 1026
HpyF3I CTNAG 2 cut(s) 281, 1052
Hsp92I GRCGYC 1 cut(s) 294
Hsp92II CATG 3 cut(s) 443, 960, 1202
HspAI GCGC 2 cut(s) 174, 294
KasI GGCGCC 1 cut(s) 293
Kpn2I TCCGGA 1 cut(s) 220
Kzo9I GATC 4 cut(s) 43, 309, 364, 592
LmnI GCTCC 1 cut(s) 1218
Lsp1109I GCAGC 2 cut(s) 183, 1197
LweI GCATC 3 cut(s) 185, 312, 904
MaeI CTAG 1 cut(s) 917
MaeIII GTNAC 3 cut(s) 148, 776, 936
MalI GATC 4 cut(s) 45, 311, 366, 594
MboI GATC 4 cut(s) 43, 309, 364, 592
MboII GAAGA 8 cut(s) 38, 53, 336, 649, 690, 837, 1088, 1173
MflI RGATCY 2 cut(s) 43, 309
Mly113I GGCGCC 1 cut(s) 294
MlyI GAGTC 2 cut(s) 887, 1243
MroI TCCGGA 1 cut(s) 220
MroXI GAANNNNTTC 1 cut(s) 45
MseI TTAA 2 cut(s) 579, 711
MspI CCGG 2 cut(s) 221, 781
MspR9I CCNGG 1 cut(s) 721
Mva1269I GAATGC 2 cut(s) 468, 601
MvaI CCWGG 1 cut(s) 721
MwoI GCNNNNNNNGC 4 cut(s) 171, 291, 300, 1026
NarI GGCGCC 1 cut(s) 294
NdeI CATATG 1 cut(s) 434
NdeII GATC 4 cut(s) 43, 309, 364, 592
NlaIII CATG 3 cut(s) 443, 960, 1202
NlaIV GGNNCC 3 cut(s) 295, 692, 1179
NmeAIII GCCGAG 1 cut(s) 191
NmuCI GTSAC 2 cut(s) 148, 776
NspI RCATGY 1 cut(s) 443
PagI TCATGA 1 cut(s) 1198
PasI CCCWGGG 1 cut(s) 720
PctI GAATGC 2 cut(s) 468, 601
PdmI GAANNNNTTC 1 cut(s) 45
PfeI GAWTC 4 cut(s) 480, 501, 640, 1082
PflFI GACNNNGTC 1 cut(s) 1252
PflMI CCANNNNNTGG 1 cut(s) 114
PkrI GCNGC 2 cut(s) 173, 1212
PleI GAGTC 2 cut(s) 886, 1243
PluTI GGCGCC 1 cut(s) 297
PpsI GAGTC 2 cut(s) 886, 1243
PsiI TTATAA 1 cut(s) 656
Psp6I CCWGG 1 cut(s) 719
PspGI CCWGG 1 cut(s) 719
PspN4I GGNNCC 3 cut(s) 295, 692, 1179
PsuI RGATCY 2 cut(s) 43, 309
PsyI GACNNNGTC 1 cut(s) 1252
RsaI GTAC 2 cut(s) 563, 1050
RsaNI GTAC 2 cut(s) 562, 1049
SaqAI TTAA 2 cut(s) 579, 711
SatI GCNGC 2 cut(s) 172, 1211
Sau3AI GATC 4 cut(s) 43, 309, 364, 592
ScaI AGTACT 1 cut(s) 1050
SchI GAGTC 2 cut(s) 887, 1243
ScrFI CCNGG 1 cut(s) 721
SetI ASST 8 cut(s) 287, 666, 745, 971, 1120, 1174, 1212, 1235
SfaNI GCATC 3 cut(s) 185, 312, 904
SfcI CTRYAG 2 cut(s) 314, 1222
SfoI GGCGCC 1 cut(s) 295
SmlI CTYRAG 1 cut(s) 632
SmoI CTYRAG 1 cut(s) 632
SsiI CCGC 1 cut(s) 1091
SspDI GGCGCC 1 cut(s) 293
SspI AATATT 1 cut(s) 621
SspMI CTAG 1 cut(s) 917
StyD4I CCNGG 1 cut(s) 719
TaaI ACNGT 2 cut(s) 318, 862
TaqI TCGA 3 cut(s) 67, 848, 924
TatI WGTACW 1 cut(s) 1048
TfiI GAWTC 4 cut(s) 480, 501, 640, 1082
Tru1I TTAA 2 cut(s) 579, 711
Tru9I TTAA 2 cut(s) 579, 711
TscAI CASTG 3 cut(s) 119, 773, 969
TseFI GTSAC 2 cut(s) 148, 776
TseI GCWGC 2 cut(s) 171, 1210
Tsp45I GTSAC 2 cut(s) 148, 776
TspDTI ATGAA 5 cut(s) 133, 390, 1027, 1187, 1231
TspGWI ACGGA 1 cut(s) 725
TspRI CASTG 3 cut(s) 119, 773, 969
Tth111I GACNNNGTC 1 cut(s) 1252
Van91I CCANNNNNTGG 1 cut(s) 114
XapI RAATTY 2 cut(s) 57, 1034
XceI RCATGY 1 cut(s) 443
XmiI GTMKAC 1 cut(s) 628
XmnI GAANNNNTTC 1 cut(s) 45
XspI CTAG 1 cut(s) 917
ZrmI AGTACT 1 cut(s) 1050
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.