Rw4G006830

F-box domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0014709
Physical Location & Seq
Reverse (-)
14297706 .. 14298978
1273 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G006830.1

Sequence Viewer

Length: 1167 bp
ATGTCAGTCTCATCATCAGCCACGGACATTGCCAACAACGAAGATCTTCTATCCCAAATCCTTGTTCGATTACCCCCAAAATCCCTCCTTTGCTTTAAATGTGTGTCCAAACAGTGGCTTTCTCTCATTTCCAGCCCCGAATTCATTGTCACCCACATCCGCTGGAGAGGCATCACTTCCCCTTCTGGCCTACTCTTAAACAATGATTACTTGCACACTCCGGAGTTCCACTATGTTCCTCTGACACACAATAATCCCTCTGAACTCACTGCCCCTCCATATCTGAGCTACCTTGGTGCCCCTCGGATCAAGATCCTACAGTCTTGTAATGGTTTGCTCTTGTGTTCTTCGGCCTACCTAAAAATGATCCAAATTATGAACTCAAATACTCTGATGACTTCACACAGACAGAGGATGATTCAAACAAGAATCCATTATTCTGGTTTCAGATTCTACGTCTGCAATCCCACCACCAGGCAATTCAAGACCCTTTCTTTTCCTTTCTTTCAGTACCGCATACTTGCTCTAAATCTGGCTTTTGATCCTTTGCATTCACCGTACTTCAAAATGTGTACCAAAACTTGGAACCCATCTTCAATATCATCTTTTAATGCACCCAGGACAATATACTTCCGTGAACCTGTGTTCTGCAGGCGAGCTATTCACTGGTATAGTTCCCAGCAGACTTCTCTTTACTTTGATGTGGACAAAGAGTGCTTGAACCCAATGCCTATGCCCCAGTCTTCGATATATGGAACTGTCAATTATTTTGGGGAGTCTCGAGGCCATCTGCATCTGGTAATGTCAACAACCTTTATTCTAGAACTCGATGTGTTTGAGTTGGAGAAAGATTACTCTGTATGGTCAGTGAGGTACCATATCAATCTTGACACCATAAGAGCTACACTCCGTGGTATGTGTGGCTTTTCCAGCGACCTGTCCCTTGCTTCAGTACTGAGAGTTACTCGTGGAGAAAAGGAAGGGGAATCAGAGGCGGTGGTATTTATGATTGGTATGGAATGTATAGTTGTATCCTGCAATTTAAGTGATGGGACGCTTAAAAGACATACGGGTTTTGTTCCGTCCCGGGAAGCTGTTCACGATTTAAAGCTCCGCCATTCCTATACAGACGCTTTTCCATTCATAGAGACTCTGTCTCCCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

388

Amino Acids

44.44

Weight (kDa)

8.69

Isoelectric Point (pI)

57.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 14 - 49 5e-08 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g06280 FvH4_4g06280 FvH4_4g06280 FvH4_4g06280
malus_domestica MD16G1239800.v1.1
rosa_chinensis RchiOBHm_Chr4g0398001
rosa_laevigata RLG00000009391
rosa_multiflora Rmu_sc0003773.1_g000024
rosa_roxburghii Rroxscaffold_5G00342960
rosa_rugosa Rorug04G0005200 Rorug04G0005300
rosa_samantha Rh4AG081900 Rh4BG079600 Rh4CG089400 Rh4DG075000
rosa_wichuraiana Rw0G019320 Rw4G006830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 873
AccB1I GGYRCC 2 cut(s) 296, 873
AccB7I CCANNNNNTGG 2 cut(s) 114, 582
AccIII TCCGGA 1 cut(s) 220
AciI CCGC 4 cut(s) 160, 514, 995, 1114
AclWI GGATC 4 cut(s) 307, 314, 361, 536
AcsI RAATTY 1 cut(s) 140
AcuI CTGAAG 1 cut(s) 933
AdeI CACNNNGTG 1 cut(s) 911
AfaI GTAC 5 cut(s) 512, 560, 574, 875, 954
AfiI CCNNNNNNNGG 3 cut(s) 114, 474, 582
AgsI TTSAA 5 cut(s) 422, 484, 565, 597, 721
AjnI CCWGG 2 cut(s) 473, 617
AjuI GAANNNNNNNTTGG 2 cut(s) 48, 80
AluBI AGCT 5 cut(s) 288, 659, 902, 1094, 1111
AluI AGCT 5 cut(s) 288, 659, 902, 1094, 1111
Alw26I GTCTC 4 cut(s) 13, 783, 1142, 1161
AlwI GGATC 4 cut(s) 307, 314, 361, 536
Ama87I CYCGRG 2 cut(s) 780, 1086
Aor13HI TCCGGA 1 cut(s) 220
AoxI GGCC 3 cut(s) 187, 351, 784
ApoI RAATTY 1 cut(s) 140
ArsI GACNNNNNNTTYG 2 cut(s) 132, 164
Asp700I GAANNNNTTC 2 cut(s) 45, 1095
Asp718I GGTACC 1 cut(s) 873
AsuC2I CCSGG 2 cut(s) 1087, 1088
AsuHPI GGTGA 2 cut(s) 142, 546
AvaI CYCGRG 2 cut(s) 780, 1086
BaeGI GKGCMC 1 cut(s) 301
BanI GGYRCC 2 cut(s) 296, 873
BauI CACGAG 1 cut(s) 966
BbsI GAAGAC 1 cut(s) 735
BccI CCATC 3 cut(s) 598, 795, 1043
BciT130I CCWGG 2 cut(s) 475, 619
BciVI GTATCC 1 cut(s) 1042
BcnI CCSGG 2 cut(s) 1087, 1088
BcoDI GTCTC 4 cut(s) 13, 783, 1142, 1161
BfaI CTAG 1 cut(s) 821
BfmI CTRYAG 2 cut(s) 317, 649
BfuI GTATCC 1 cut(s) 1042
BglII AGATCT 1 cut(s) 43
BmcAI AGTACT 1 cut(s) 954
Bme1390I CCNGG 4 cut(s) 475, 619, 1087, 1088
BmeT110I CYCGRG 2 cut(s) 780, 1086
BmiI GGNNCC 3 cut(s) 298, 587, 875
BmrFI CCNGG 4 cut(s) 475, 619, 1087, 1088
BmrI ACTGGG 1 cut(s) 733
BmsI GCATC 2 cut(s) 180, 802
BmuI ACTGGG 1 cut(s) 733
BpiI GAAGAC 1 cut(s) 735
BplI GAGNNNNNCTC 4 cut(s) 891, 923, 949, 981
BpmI CTGGAG 1 cut(s) 184
BpuMI CCSGG 2 cut(s) 1087, 1088
BsaBI GATNNNNATC 1 cut(s) 311
BsaJI CCNNGG 6 cut(s) 21, 292, 302, 617, 910, 1086
BsaWI WCCGGW 1 cut(s) 220
BsaXI ACNNNNNCTCC 3 cut(s) 259, 289, 1141
Bsc4I CCNNNNNNNGG 3 cut(s) 114, 474, 582
Bse1I ACTGG 2 cut(s) 671, 739
Bse3DI GCAATG 1 cut(s) 27
Bse8I GATNNNNATC 1 cut(s) 311
BseAI TCCGGA 1 cut(s) 220
BseBI CCWGG 2 cut(s) 475, 619
BseDI CCNNGG 6 cut(s) 21, 292, 302, 617, 910, 1086
BseGI GGATG 2 cut(s) 156, 420
BseJI GATNNNNATC 1 cut(s) 311
BseLI CCNNNNNNNGG 3 cut(s) 114, 474, 582
BseMI GCAATG 1 cut(s) 27
BseMII CTCAG 2 cut(s) 275, 947
BseNI ACTGG 2 cut(s) 671, 739
BseSI GKGCMC 1 cut(s) 301
BseYI CCCAGC 1 cut(s) 678
BshFI GGCC 3 cut(s) 189, 353, 786
BshNI GGYRCC 2 cut(s) 296, 873
BsiHKCI CYCGRG 2 cut(s) 780, 1086
BsiSI CCGG 2 cut(s) 221, 1087
BslFI GGGAC 3 cut(s) 925, 1066, 1069
BslI CCNNNNNNNGG 3 cut(s) 114, 474, 582
BsmAI GTCTC 4 cut(s) 13, 783, 1142, 1161
BsmFI GGGAC 3 cut(s) 925, 1066, 1069
BsmI GAATGC 1 cut(s) 550
BsnI GGCC 3 cut(s) 189, 353, 786
BsoBI CYCGRG 2 cut(s) 780, 1086
Bsp1286I GDGCHC 1 cut(s) 301
Bsp13I TCCGGA 1 cut(s) 220
Bsp143I GATC 5 cut(s) 43, 306, 312, 366, 541
BspACI CCGC 4 cut(s) 160, 514, 995, 1114
BspANI GGCC 3 cut(s) 189, 353, 786
BspCNI CTCAG 2 cut(s) 276, 948
BspEI TCCGGA 1 cut(s) 220
BspLI GGNNCC 3 cut(s) 298, 587, 875
BspMAI CTGCAG 1 cut(s) 653
BspPI GGATC 4 cut(s) 307, 314, 361, 536
BspT107I GGYRCC 2 cut(s) 296, 873
BsrDI GCAATG 1 cut(s) 27
BsrI ACTGG 2 cut(s) 671, 739
BssECI CCNNGG 6 cut(s) 21, 292, 302, 617, 910, 1086
BssMI GATC 5 cut(s) 43, 306, 312, 366, 541
BssSI CACGAG 1 cut(s) 966
BssT1I CCWWGG 1 cut(s) 292
Bst2BI CACGAG 1 cut(s) 966
Bst2UI CCWGG 2 cut(s) 475, 619
Bst4CI ACNGT 4 cut(s) 114, 321, 558, 760
BstC8I GCNNGC 2 cut(s) 653, 657
BstDEI CTNAG 2 cut(s) 284, 956
BstDSI CCRYGG 2 cut(s) 21, 910
BstF5I GGATG 2 cut(s) 156, 420
BstKTI GATC 5 cut(s) 46, 309, 315, 369, 544
BstMAI GTCTC 4 cut(s) 13, 783, 1142, 1161
BstMBI GATC 5 cut(s) 43, 306, 312, 366, 541
BstMWI GCNNNNNNNGC 2 cut(s) 168, 930
BstNI CCWGG 2 cut(s) 475, 619
BstSCI CCNGG 4 cut(s) 473, 617, 1085, 1086
BstSFI CTRYAG 2 cut(s) 317, 649
BstSLI GKGCMC 1 cut(s) 301
BstV2I GAAGAC 1 cut(s) 735
BstX2I RGATCY 2 cut(s) 43, 312
BstXI CCANNNNNNTGG 1 cut(s) 440
BstYI RGATCY 2 cut(s) 43, 312
BsuI GTATCC 1 cut(s) 1042
BsuRI GGCC 3 cut(s) 189, 353, 786
BtgI CCRYGG 2 cut(s) 21, 910
BtsCI GGATG 2 cut(s) 156, 420
BtsI GCAGTG 1 cut(s) 267
BtsIMutI CAGTG 4 cut(s) 119, 267, 664, 873
Cac8I GCNNGC 2 cut(s) 653, 657
Cfr9I CCCGGG 1 cut(s) 1086
CseI GACGC 2 cut(s) 1063, 1139
Csp6I GTAC 5 cut(s) 511, 559, 573, 874, 953
CspCI CAANNNNNGTGG 2 cut(s) 10, 45
CviQI GTAC 5 cut(s) 511, 559, 573, 874, 953
DdeI CTNAG 2 cut(s) 284, 956
DpnI GATC 5 cut(s) 45, 308, 314, 368, 543
DpnII GATC 5 cut(s) 43, 306, 312, 366, 541
DraI TTTAAA 2 cut(s) 97, 1107
DraIII CACNNNGTG 1 cut(s) 911
EciI GGCGGA 1 cut(s) 1103
Eco130I CCWWGG 1 cut(s) 292
Eco57I CTGAAG 1 cut(s) 933
Eco88I CYCGRG 2 cut(s) 780, 1086
EcoRI GAATTC 1 cut(s) 140
EcoRII CCWGG 2 cut(s) 473, 617
EcoT14I CCWWGG 1 cut(s) 292
ErhI CCWWGG 1 cut(s) 292
FaqI GGGAC 3 cut(s) 925, 1066, 1069
FokI GGATG 2 cut(s) 143, 427
FspBI CTAG 1 cut(s) 821
GsaI CCCAGC 1 cut(s) 682
GsuI CTGGAG 1 cut(s) 184
HaeIII GGCC 3 cut(s) 189, 353, 786
HapII CCGG 2 cut(s) 221, 1087
HgaI GACGC 2 cut(s) 1063, 1139
HincII GTYRAC 1 cut(s) 807
HindII GTYRAC 1 cut(s) 807
HinfI GANTC 6 cut(s) 418, 429, 450, 776, 986, 1150
HpaII CCGG 2 cut(s) 221, 1087
HphI GGTGA 2 cut(s) 142, 546
Hpy166II GTNNAC 5 cut(s) 573, 638, 706, 807, 1099
Hpy188I TCNGA 7 cut(s) 243, 262, 285, 306, 393, 449, 991
Hpy188III TCNNGA 7 cut(s) 221, 310, 484, 780, 821, 887, 1100
Hpy8I GTNNAC 5 cut(s) 573, 638, 706, 807, 1099
HpyAV CCTTC 2 cut(s) 192, 974
HpyCH4III ACNGT 4 cut(s) 114, 321, 558, 760
HpyCH4IV ACGT 1 cut(s) 456
HpyCH4V TGCA 7 cut(s) 214, 462, 550, 614, 651, 793, 1038
HpyF10VI GCNNNNNNNGC 2 cut(s) 168, 930
HpyF3I CTNAG 2 cut(s) 284, 956
HpySE526I ACGT 1 cut(s) 456
Kpn2I TCCGGA 1 cut(s) 220
KpnI GGTACC 1 cut(s) 877
Kzo9I GATC 5 cut(s) 43, 306, 312, 366, 541
LmnI GCTCC 1 cut(s) 1116
LweI GCATC 2 cut(s) 180, 802
MaeI CTAG 1 cut(s) 821
MaeII ACGT 1 cut(s) 456
MaeIII GTNAC 2 cut(s) 148, 961
MalI GATC 5 cut(s) 45, 308, 314, 368, 543
MboI GATC 5 cut(s) 43, 306, 312, 366, 541
MboII GAAGA 5 cut(s) 38, 53, 339, 585, 735
MflI RGATCY 2 cut(s) 43, 312
MhlI GDGCHC 1 cut(s) 301
MluCI AATT 5 cut(s) 140, 372, 479, 763, 1039
MlyI GAGTC 2 cut(s) 785, 1144
MmeI TCCRAC 1 cut(s) 822
MroI TCCGGA 1 cut(s) 220
MroXI GAANNNNTTC 2 cut(s) 45, 1095
MseI TTAA 6 cut(s) 96, 197, 609, 1043, 1059, 1106
MspA1I CMGCKG 1 cut(s) 162
MspI CCGG 2 cut(s) 221, 1087
MspR9I CCNGG 4 cut(s) 475, 619, 1087, 1088
Mva1269I GAATGC 1 cut(s) 550
MvaI CCWGG 2 cut(s) 475, 619
MwoI GCNNNNNNNGC 2 cut(s) 168, 930
NciI CCSGG 2 cut(s) 1087, 1088
NdeII GATC 5 cut(s) 43, 306, 312, 366, 541
NlaIV GGNNCC 3 cut(s) 298, 587, 875
NmuCI GTSAC 1 cut(s) 148
PaeR7I CTCGAG 1 cut(s) 780
PctI GAATGC 1 cut(s) 550
PdmI GAANNNNTTC 2 cut(s) 45, 1095
PfeI GAWTC 4 cut(s) 418, 429, 450, 986
PflFI GACNNNGTC 1 cut(s) 1153
PflMI CCANNNNNTGG 2 cut(s) 114, 582
PleI GAGTC 2 cut(s) 784, 1144
PpsI GAGTC 2 cut(s) 784, 1144
Psp6I CCWGG 2 cut(s) 473, 617
PspFI CCCAGC 1 cut(s) 678
PspGI CCWGG 2 cut(s) 473, 617
PspN4I GGNNCC 3 cut(s) 298, 587, 875
PstI CTGCAG 1 cut(s) 653
PsuI RGATCY 2 cut(s) 43, 312
PsyI GACNNNGTC 1 cut(s) 1153
RsaI GTAC 5 cut(s) 512, 560, 574, 875, 954
RsaNI GTAC 5 cut(s) 511, 559, 573, 874, 953
SaqAI TTAA 6 cut(s) 96, 197, 609, 1043, 1059, 1106
Sau3AI GATC 5 cut(s) 43, 306, 312, 366, 541
ScaI AGTACT 1 cut(s) 954
SchI GAGTC 2 cut(s) 785, 1144
ScrFI CCNGG 4 cut(s) 475, 619, 1087, 1088
SduI GDGCHC 1 cut(s) 301
SfaNI GCATC 2 cut(s) 180, 802
SfcI CTRYAG 2 cut(s) 317, 649
Sfr274I CTCGAG 1 cut(s) 780
SlaI CTCGAG 1 cut(s) 780
SmaI CCCGGG 1 cut(s) 1088
SmlI CTYRAG 1 cut(s) 780
SmoI CTYRAG 1 cut(s) 780
Sse9I AATT 5 cut(s) 140, 372, 479, 763, 1039
SsiI CCGC 4 cut(s) 160, 514, 995, 1114
SspMI CTAG 1 cut(s) 821
StyD4I CCNGG 4 cut(s) 473, 617, 1085, 1086
StyI CCWWGG 1 cut(s) 292
TaaI ACNGT 4 cut(s) 114, 321, 558, 760
TaiI ACGT 1 cut(s) 459
TaqI TCGA 4 cut(s) 67, 746, 781, 828
TasI AATT 5 cut(s) 140, 372, 479, 763, 1039
TatI WGTACW 1 cut(s) 952
TfiI GAWTC 4 cut(s) 418, 429, 450, 986
Tru1I TTAA 6 cut(s) 96, 197, 609, 1043, 1059, 1106
Tru9I TTAA 6 cut(s) 96, 197, 609, 1043, 1059, 1106
TscAI CASTG 4 cut(s) 119, 274, 671, 873
TseFI GTSAC 1 cut(s) 148
Tsp45I GTSAC 1 cut(s) 148
TspDTI ATGAA 3 cut(s) 133, 392, 1132
TspGWI ACGGA 4 cut(s) 38, 623, 899, 1071
TspMI CCCGGG 1 cut(s) 1086
TspRI CASTG 4 cut(s) 119, 274, 671, 873
Tth111I GACNNNGTC 1 cut(s) 1153
Van91I CCANNNNNTGG 2 cut(s) 114, 582
XapI RAATTY 1 cut(s) 140
XbaI TCTAGA 1 cut(s) 820
XhoI CTCGAG 1 cut(s) 780
XmaI CCCGGG 1 cut(s) 1086
XmnI GAANNNNTTC 2 cut(s) 45, 1095
XspI CTAG 1 cut(s) 821
ZrmI AGTACT 1 cut(s) 954
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.