MD16G1239800.v1.1

F-box domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014709
Physical Location & Seq
Reverse (-)
25475454 .. 25476677
1224 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1239800.v1.1.491

Sequence Viewer

Length: 1224 bp
ATGGCAATCTCATCGCCCGGAGAAGCCGTCGCCAACAGCCAAGACATACTCACACAAATCCTCCTCCGACTGCCCACAAAATCCCTCGTCCGCTTCAAATGCGTATCAACCCACTGGCTCTCTCTCATCTCCTGCCCCCAATTCATCGCCGCCCAAGTCCGCTGCCAAGGTACTCTCGCCCCCTCCGGCCTCCTCTTGAACAATGACTACTTGCCCACCCCAGAATTCCAATATGTCTCTTTCACTCACTCGAATCACCCCAAATTCGATGCGAATCCCCCTCCGTATCTGAGTTATCTGGGTGTTCCACGCATCAAAATCCTTCAATCCTGTAATGGGTTGCTCTTGTGCTCCTCTGCCTACCTTACGGACGACCCCGATTTCAAGAACACAGCGTTTGATTTTGGTATCCCACATGATTTTATTACTTCTGTTAGTGAGGATGATTGTGGGATTAGAATCTTTGTTTGTAATCCCATCACCATGCAGTTCAAGACCATTAATCTTCCGAGCGATGAAGTTGGTTGCGCAGAGATAGAGGGTGTGAATATGGCTTTTGATCCGACTCATTCACCCCACTTCAAGATAGTGTGTGTACTGAGGAGCTCGAATGGCGCTGACGAAGTATATTCGCTTCGTGTTTATGAATCCCAGAACAACGCTTGGAAGCCGTCTCTAATCAATTTTGATCCACCAGAAGCAACACATTTCGGTGCCCCTGTGTTTTTTAGGCGGGCAATTCACTGGTACAGTGAGGAGCAGACTTCCCTTTGTTTCGATGTGGACGAAGAGTGCTTGAACCCGATTCCCATGCCGCGATACCTAACCCGTAACACTATCCAGTATTTTGGGGAGTCTCGGGGCCAGCTGCAGTTGATAATGCACACGACCTCGGATGTGGAATTTGATGTGTTGGAGTTGGAGGAGGATTACTCTGGTTGGCGGCTGAGGCACCGTGTCAATCTTGCTACCTTTAGAGTTGAATTCTGTGATATGTATGGGGTTGGCAGCGAATGGTTTCGTGTTTCGGTTTTGAGAATGGTTGAAGCAGAAGCAGAAAGGCAGGTGGAATCGGAACTGGTGCTATTTGTTTGTGGTAGGTTATTCTCTTACAGATTGAAGGATGGTTCGTTGAAGCAGCTCATGTGTCTAGTTCCATATGAGAAAGCTGAGTGTGAATGGGAGAATGCCTTTCCATTCATAGAGACTCTCTGTCCTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

408

Amino Acids

46.35

Weight (kDa)

4.94

Isoelectric Point (pI)

44.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 14 - 46 8.6e-07 F-box domain
FBA_3 PF08268 150 - 314 4.6e-07 F-box associated beta propeller domain
FBA_1 PF07734 152 - 272 1.7e-08 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g06280 FvH4_4g06280 FvH4_4g06280 FvH4_4g06280
malus_domestica MD16G1239800.v1.1
rosa_chinensis RchiOBHm_Chr4g0398001
rosa_laevigata RLG00000009391
rosa_multiflora Rmu_sc0003773.1_g000024
rosa_roxburghii Rroxscaffold_5G00342960
rosa_rugosa Rorug04G0005200 Rorug04G0005300
rosa_samantha Rh4AG081900 Rh4BG079600 Rh4CG089400 Rh4DG075000
rosa_wichuraiana Rw0G019320 Rw4G006830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1054
Acc16I TGCGCA 1 cut(s) 529
Acc36I ACCTGC 1 cut(s) 1054
AccB1I GGYRCC 2 cut(s) 713, 951
AccII CGCG 1 cut(s) 817
AciI CCGC 6 cut(s) 91, 150, 160, 733, 815, 943
AclWI GGATC 2 cut(s) 554, 683
AcsI RAATTY 4 cut(s) 224, 263, 902, 983
AfaI GTAC 3 cut(s) 172, 597, 749
AfiI CCNNNNNNNGG 1 cut(s) 336
AhdI GACNNNNNGTC 1 cut(s) 1212
AluBI AGCT 4 cut(s) 606, 868, 1141, 1169
AluI AGCT 4 cut(s) 606, 868, 1141, 1169
Alw21I GWGCWC 2 cut(s) 353, 608
Alw26I GTCTC 4 cut(s) 241, 678, 861, 1199
AlwI GGATC 2 cut(s) 554, 683
Ama87I CYCGRG 1 cut(s) 858
AoxI GGCC 2 cut(s) 187, 862
ApeKI GCWGC 4 cut(s) 162, 868, 1008, 1138
ApoI RAATTY 4 cut(s) 224, 263, 902, 983
AseI ATTAAT 1 cut(s) 501
Asp700I GAANNNNTTC 1 cut(s) 1017
AspLEI GCGC 2 cut(s) 530, 617
AspS9I GGNCC 1 cut(s) 862
AsuC2I CCSGG 1 cut(s) 18
AsuHPI GGTGA 3 cut(s) 248, 472, 564
AvaI CYCGRG 1 cut(s) 858
BaeGI GKGCMC 1 cut(s) 718
BanI GGYRCC 2 cut(s) 713, 951
BanII GRGCYC 1 cut(s) 608
BarI GAAGNNNNNNTAC 2 cut(s) 618, 650
Bbv12I GWGCWC 2 cut(s) 353, 608
BbvCI CCTCAGC 1 cut(s) 947
BbvI GCAGC 4 cut(s) 149, 855, 1020, 1150
BccI CCATC 2 cut(s) 485, 1118
BceAI ACGGC 2 cut(s) 11, 655
BcgI CGANNNNNNTGC 3 cut(s) 28, 793, 827
BciVI GTATCC 1 cut(s) 419
BcnI CCSGG 1 cut(s) 18
BcoDI GTCTC 4 cut(s) 241, 678, 861, 1199
BfaI CTAG 1 cut(s) 1151
BfmI CTRYAG 1 cut(s) 869
BfoI RGCGCY 1 cut(s) 618
BfuAI ACCTGC 1 cut(s) 1054
BfuI GTATCC 1 cut(s) 419
BisI GCNGC 7 cut(s) 150, 163, 815, 869, 944, 1009, 1139
BlsI GCNGC 7 cut(s) 151, 164, 816, 870, 945, 1010, 1140
Bme1390I CCNGG 1 cut(s) 18
BmeRI GACNNNNNGTC 1 cut(s) 1212
BmeT110I CYCGRG 1 cut(s) 858
BmgT120I GGNCC 1 cut(s) 862
BmiI GGNNCC 3 cut(s) 715, 863, 953
BmrFI CCNGG 1 cut(s) 18
BmsI GCATC 2 cut(s) 259, 321
BplI GAGNNNNNCTC 2 cut(s) 917, 949
Bpu10I CCTNAGC 1 cut(s) 947
BpuMI CCSGG 1 cut(s) 18
BsaBI GATNNNNATC 2 cut(s) 273, 458
BsaJI CCNNGG 2 cut(s) 166, 891
BsaXI ACNNNNNCTCC 4 cut(s) 12, 42, 45, 75
Bsc4I CCNNNNNNNGG 1 cut(s) 336
Bse1I ACTGG 4 cut(s) 119, 749, 841, 1083
Bse8I GATNNNNATC 2 cut(s) 273, 458
BseDI CCNNGG 2 cut(s) 166, 891
BseGI GGATG 3 cut(s) 448, 901, 1129
BseJI GATNNNNATC 2 cut(s) 273, 458
BseLI CCNNNNNNNGG 1 cut(s) 336
BseMII CTCAG 4 cut(s) 281, 590, 938, 1161
BseNI ACTGG 4 cut(s) 119, 749, 841, 1083
BseRI GAGGAG 6 cut(s) 53, 182, 343, 616, 770, 938
BseSI GKGCMC 1 cut(s) 718
BseXI GCAGC 4 cut(s) 149, 855, 1020, 1150
Bsh1236I CGCG 1 cut(s) 817
BshFI GGCC 2 cut(s) 189, 864
BshNI GGYRCC 2 cut(s) 713, 951
BsiHKAI GWGCWC 2 cut(s) 353, 608
BsiHKCI CYCGRG 1 cut(s) 858
BsiSI CCGG 2 cut(s) 18, 186
BslI CCNNNNNNNGG 1 cut(s) 336
BsmAI GTCTC 4 cut(s) 241, 678, 861, 1199
BsmBI CGTCTC 1 cut(s) 678
BsmI GAATGC 1 cut(s) 1192
BsnI GGCC 2 cut(s) 189, 864
BsoBI CYCGRG 1 cut(s) 858
Bsp1286I GDGCHC 3 cut(s) 353, 608, 718
Bsp143I GATC 2 cut(s) 559, 688
BspACI CCGC 6 cut(s) 91, 150, 160, 733, 815, 943
BspANI GGCC 2 cut(s) 189, 864
BspCNI CTCAG 4 cut(s) 282, 591, 939, 1162
BspFNI CGCG 1 cut(s) 817
BspLI GGNNCC 3 cut(s) 715, 863, 953
BspMAI CTGCAG 1 cut(s) 873
BspMI ACCTGC 1 cut(s) 1054
BspPI GGATC 2 cut(s) 554, 683
BspT107I GGYRCC 2 cut(s) 713, 951
BsrI ACTGG 4 cut(s) 119, 749, 841, 1083
BssECI CCNNGG 2 cut(s) 166, 891
BssMI GATC 2 cut(s) 559, 688
BssT1I CCWWGG 1 cut(s) 166
Bst4CI ACNGT 2 cut(s) 752, 956
Bst6I CTCTTC 1 cut(s) 783
BstC8I GCNNGC 2 cut(s) 735, 866
BstDEI CTNAG 4 cut(s) 290, 599, 947, 1170
BstF5I GGATG 3 cut(s) 448, 901, 1129
BstFNI CGCG 1 cut(s) 817
BstH2I RGCGCY 1 cut(s) 618
BstHHI GCGC 2 cut(s) 530, 617
BstKTI GATC 2 cut(s) 562, 691
BstMAI GTCTC 4 cut(s) 241, 678, 861, 1199
BstMBI GATC 2 cut(s) 559, 688
BstMWI GCNNNNNNNGC 3 cut(s) 99, 612, 949
BstSCI CCNGG 1 cut(s) 16
BstSFI CTRYAG 1 cut(s) 869
BstSLI GKGCMC 1 cut(s) 718
BstUI CGCG 1 cut(s) 817
BstV1I GCAGC 4 cut(s) 149, 855, 1020, 1150
BstXI CCANNNNNNTGG 1 cut(s) 848
BsuI GTATCC 1 cut(s) 419
BsuRI GGCC 2 cut(s) 189, 864
BtgZI GCGATG 2 cut(s) 130, 528
BtsCI GGATG 3 cut(s) 448, 901, 1129
BtsIMutI CAGTG 3 cut(s) 112, 742, 757
BveI ACCTGC 1 cut(s) 1054
Cac8I GCNNGC 2 cut(s) 735, 866
CfoI GCGC 2 cut(s) 530, 617
Cfr13I GGNCC 1 cut(s) 862
Csp6I GTAC 3 cut(s) 171, 596, 748
CviAII CATG 4 cut(s) 416, 484, 811, 1144
CviQI GTAC 3 cut(s) 171, 596, 748
DdeI CTNAG 4 cut(s) 290, 599, 947, 1170
DpnI GATC 2 cut(s) 561, 690
DpnII GATC 2 cut(s) 559, 688
DriI GACNNNNNGTC 1 cut(s) 1212
Eam1104I CTCTTC 1 cut(s) 783
Eam1105I GACNNNNNGTC 1 cut(s) 1212
EarI CTCTTC 1 cut(s) 783
Ecl136II GAGCTC 1 cut(s) 606
Eco130I CCWWGG 1 cut(s) 166
Eco24I GRGCYC 1 cut(s) 608
Eco53kI GAGCTC 1 cut(s) 606
Eco88I CYCGRG 1 cut(s) 858
EcoICRI GAGCTC 1 cut(s) 606
EcoRI GAATTC 2 cut(s) 224, 983
EcoT14I CCWWGG 1 cut(s) 166
EcoT38I GRGCYC 1 cut(s) 608
ErhI CCWWGG 1 cut(s) 166
Esp3I CGTCTC 1 cut(s) 678
FaeI CATG 4 cut(s) 419, 487, 814, 1147
FatI CATG 4 cut(s) 415, 483, 810, 1143
FauI CCCGC 1 cut(s) 726
FauNDI CATATG 1 cut(s) 1159
Fnu4HI GCNGC 7 cut(s) 150, 163, 815, 869, 944, 1009, 1139
FokI GGATG 3 cut(s) 455, 908, 1136
FriOI GRGCYC 1 cut(s) 608
Fsp4HI GCNGC 7 cut(s) 150, 163, 815, 869, 944, 1009, 1139
FspBI CTAG 1 cut(s) 1151
FspI TGCGCA 1 cut(s) 529
GlaI GCGC 2 cut(s) 529, 616
GluI GCNGC 7 cut(s) 150, 163, 815, 869, 944, 1009, 1139
HaeII RGCGCY 1 cut(s) 618
HaeIII GGCC 2 cut(s) 189, 864
HapII CCGG 2 cut(s) 18, 186
HhaI GCGC 2 cut(s) 530, 617
Hin1II CATG 4 cut(s) 419, 487, 814, 1147
Hin6I GCGC 2 cut(s) 528, 615
HinP1I GCGC 2 cut(s) 528, 615
HinfI GANTC 9 cut(s) 253, 274, 459, 565, 647, 805, 854, 1070, 1207
HpaII CCGG 2 cut(s) 18, 186
HphI GGTGA 3 cut(s) 248, 472, 564
Hpy166II GTNNAC 2 cut(s) 596, 784
Hpy188I TCNGA 6 cut(s) 68, 291, 510, 564, 895, 1075
Hpy188III TCNNGA 4 cut(s) 196, 385, 493, 583
Hpy8I GTNNAC 2 cut(s) 596, 784
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 2 cut(s) 332, 1114
HpyCH4III ACNGT 2 cut(s) 752, 956
HpyCH4V TGCA 3 cut(s) 487, 871, 883
HpyF10VI GCNNNNNNNGC 3 cut(s) 99, 612, 949
HpyF3I CTNAG 4 cut(s) 290, 599, 947, 1170
Hsp92II CATG 4 cut(s) 419, 487, 814, 1147
HspAI GCGC 2 cut(s) 528, 615
Kzo9I GATC 2 cut(s) 559, 688
LmnI GCTCC 3 cut(s) 356, 603, 757
Lsp1109I GCAGC 4 cut(s) 149, 855, 1020, 1150
LweI GCATC 2 cut(s) 259, 321
MaeI CTAG 1 cut(s) 1151
MaeIII GTNAC 1 cut(s) 830
MalI GATC 2 cut(s) 561, 690
MboI GATC 2 cut(s) 559, 688
MboII GAAGA 2 cut(s) 497, 800
MhlI GDGCHC 3 cut(s) 353, 608, 718
MluCI AATT 7 cut(s) 140, 224, 263, 682, 738, 902, 983
MlyI GAGTC 3 cut(s) 559, 863, 1201
MmeI TCCRAC 4 cut(s) 91, 587, 894, 900
MroXI GAANNNNTTC 1 cut(s) 1017
MseI TTAA 1 cut(s) 501
MslI CAYNNNNRTG 2 cut(s) 482, 711
MspA1I CMGCKG 2 cut(s) 162, 868
MspI CCGG 2 cut(s) 18, 186
MspR9I CCNGG 1 cut(s) 18
Mva1269I GAATGC 1 cut(s) 1192
MvnI CGCG 1 cut(s) 817
MwoI GCNNNNNNNGC 3 cut(s) 99, 612, 949
NciI CCSGG 1 cut(s) 18
NdeI CATATG 1 cut(s) 1159
NdeII GATC 2 cut(s) 559, 688
NlaIII CATG 4 cut(s) 419, 487, 814, 1147
NlaIV GGNNCC 3 cut(s) 715, 863, 953
NsbI TGCGCA 1 cut(s) 529
PaqCI CACCTGC 1 cut(s) 1054
PcsI WCGNNNNNNNCGW 1 cut(s) 783
PctI GAATGC 1 cut(s) 1192
PdmI GAANNNNTTC 1 cut(s) 1017
PfeI GAWTC 6 cut(s) 253, 274, 459, 647, 805, 1070
PkrI GCNGC 7 cut(s) 151, 164, 816, 870, 945, 1010, 1140
PleI GAGTC 3 cut(s) 559, 862, 1201
PpsI GAGTC 3 cut(s) 559, 862, 1201
PshBI ATTAAT 1 cut(s) 501
Psp124BI GAGCTC 1 cut(s) 608
PspN4I GGNNCC 3 cut(s) 715, 863, 953
PspPI GGNCC 1 cut(s) 862
PsrI GAACNNNNNNTAC 2 cut(s) 191, 223
PstI CTGCAG 1 cut(s) 873
PvuII CAGCTG 1 cut(s) 868
RsaI GTAC 3 cut(s) 172, 597, 749
RsaNI GTAC 3 cut(s) 171, 596, 748
RseI CAYNNNNRTG 2 cut(s) 482, 711
SacI GAGCTC 1 cut(s) 608
SaqAI TTAA 1 cut(s) 501
SatI GCNGC 7 cut(s) 150, 163, 815, 869, 944, 1009, 1139
Sau3AI GATC 2 cut(s) 559, 688
Sau96I GGNCC 1 cut(s) 862
SchI GAGTC 3 cut(s) 559, 863, 1201
ScrFI CCNGG 1 cut(s) 18
SduI GDGCHC 3 cut(s) 353, 608, 718
SfaNI GCATC 2 cut(s) 259, 321
SfcI CTRYAG 1 cut(s) 869
SmiMI CAYNNNNRTG 2 cut(s) 482, 711
Sse9I AATT 7 cut(s) 140, 224, 263, 682, 738, 902, 983
SsiI CCGC 6 cut(s) 91, 150, 160, 733, 815, 943
SspMI CTAG 1 cut(s) 1151
SstI GAGCTC 1 cut(s) 608
StyD4I CCNGG 1 cut(s) 16
StyI CCWWGG 1 cut(s) 166
TaaI ACNGT 2 cut(s) 752, 956
TaqI TCGA 4 cut(s) 251, 267, 608, 777
TasI AATT 7 cut(s) 140, 224, 263, 682, 738, 902, 983
TatI WGTACW 1 cut(s) 595
TauI GCSGC 3 cut(s) 152, 817, 946
TfiI GAWTC 6 cut(s) 253, 274, 459, 647, 805, 1070
Tru1I TTAA 1 cut(s) 501
Tru9I TTAA 1 cut(s) 501
TscAI CASTG 3 cut(s) 119, 749, 757
TseI GCWGC 4 cut(s) 162, 868, 1008, 1138
TspDTI ATGAA 4 cut(s) 133, 531, 660, 1189
TspGWI ACGGA 2 cut(s) 273, 383
TspRI CASTG 3 cut(s) 119, 749, 757
VspI ATTAAT 1 cut(s) 501
XapI RAATTY 4 cut(s) 224, 263, 902, 983
XmnI GAANNNNTTC 1 cut(s) 1017
XspI CTAG 1 cut(s) 1151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.