Rroxscaffold_5G00342960

F-box domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0014709
Physical Location & Seq
Reverse (-)
12118953 .. 12121719
2767 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00342960.1

Sequence Viewer

Length: 1272 bp
ATGTCAGTCTCATCATCAGCCACGGACATTGCCAACAACGAAGATCTTCTATCCCAAATCCTTGTTCGATTACCCCCAAAATCCCTCCTTTGCTTCAAATGTGTGTCCAAACAGTGGCTTTCTCTCATTTCCAGCCCCGAATTCATTGTCACCCACATCCGCTGGAGAGGCATCGCTTCCCCTTCTGGCCTACTCTTAAACAATGACTACTTGCACACTCCGGAGTTCCACTATGTTCCTCTGACACACAATAATCCCTCTGAACTCACTGCCCCTCCATATCTGAGATACCTTGGTGCCCCTCGGATCAAGATCCTACAGTCTTGTAATGGTTTGCTCTTGTGTTCTTCGGCCTACCTAAAAAATGATCCAAATTATGAACTCAAATACTCTGATGACTTCACGCAGACAGAGGATGATGTCCTCTTGCCAGATATATGGCATGTTATCCCAACAATCTCAAACTCCTGCAGTCAAACAAGAATCCATTATTCTGGTTTCAGATTCTACGTCTGCAATCCCACCACCAGGCAATTCAAGACCCTTTCTTTTCCTTTCTTTCAGTACCACATACTTGCTCTAAATCTGGCTTTTGATCCTCTGCATTCACCGTACTTCAAAATAGTGTGTGTACTTCGGAAGAATCTGGATACTTATATACTTCACCTATATAATTCTGAGACCAAAACTTGGAACCCATCTTCAATATCTTTTAATGCACCCAGGACAATATACTTCCGTGAACCTGTGTTCTGCAGGCAAGCAATTCACTGGTATAGTTCCCAGCAGACTTCTCTTTACTTTGATGTGGACAAAGAGTGCTTGAACCCAATGCCTATGCCCCAGTCTTCGATATATGGAACTGTCAATTATTTTGGGGAGTCTCGAGGCCATCTGCATCTGGTAATGTCAACAACCTCTATTCTAGAACTCGATGTGTTTGAGTTGGAGAAAGATTACTCTGTATGGTCAGTGAGGTACCATATCAATCTTGACACCATAAGAGCTACACTCCGTGGTATGTGTGGCTTTTCCAGCGACCCGTCCCTTGCTTCAGTACTGAGAGTTACTCGTGGAGAAAAGGAAGGGGAATCAGAGGCGGTGGTATTTATGTTTGGTATGGAATGTATAGTTGTATCTTGCAATTTGAGTGATGGGAAGCTTAAAAGACATACAGGTTTTGTTCCTTCACGGGAAGCTGTTCACGATTTAAAGCTCCTCCATTCCTATACAGACGCCTTTCCATTCATAGAGACTCTGTCTCCCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

423

Amino Acids

48.42

Weight (kDa)

6.46

Isoelectric Point (pI)

53.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 14 - 49 5.6e-08 F-box domain
FBA_3 PF08268 162 - 324 5.2e-06 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g06280 FvH4_4g06280 FvH4_4g06280 FvH4_4g06280
malus_domestica MD16G1239800.v1.1
rosa_chinensis RchiOBHm_Chr4g0398001
rosa_laevigata RLG00000009391
rosa_multiflora Rmu_sc0003773.1_g000024
rosa_roxburghii Rroxscaffold_5G00342960
rosa_rugosa Rorug04G0005200 Rorug04G0005300
rosa_samantha Rh4AG081900 Rh4BG079600 Rh4CG089400 Rh4DG075000
rosa_wichuraiana Rw0G019320 Rw4G006830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 978
AccB1I GGYRCC 2 cut(s) 296, 978
AccB7I CCANNNNNTGG 2 cut(s) 114, 690
AccIII TCCGGA 1 cut(s) 220
AciI CCGC 2 cut(s) 160, 1100
AclWI GGATC 4 cut(s) 307, 314, 362, 590
AcsI RAATTY 1 cut(s) 140
AcuI CTGAAG 1 cut(s) 1038
AcyI GRCGYC 1 cut(s) 1236
AdeI CACNNNGTG 1 cut(s) 1016
AfaI GTAC 5 cut(s) 566, 614, 633, 980, 1059
AfiI CCNNNNNNNGG 3 cut(s) 114, 528, 690
AgsI TTSAA 5 cut(s) 97, 538, 619, 705, 826
AjnI CCWGG 2 cut(s) 527, 722
AjuI GAANNNNNNNTTGG 2 cut(s) 48, 80
AluBI AGCT 4 cut(s) 1007, 1162, 1199, 1216
AluI AGCT 4 cut(s) 1007, 1162, 1199, 1216
Alw26I GTCTC 5 cut(s) 13, 674, 888, 1247, 1266
AlwI GGATC 4 cut(s) 307, 314, 362, 590
Ama87I CYCGRG 1 cut(s) 885
Aor13HI TCCGGA 1 cut(s) 220
AoxI GGCC 3 cut(s) 187, 351, 889
ApoI RAATTY 1 cut(s) 140
ArsI GACNNNNNNTTYG 2 cut(s) 132, 164
Asp700I GAANNNNTTC 2 cut(s) 45, 1200
Asp718I GGTACC 1 cut(s) 978
AsuHPI GGTGA 3 cut(s) 142, 600, 656
AvaI CYCGRG 1 cut(s) 885
BaeGI GKGCMC 1 cut(s) 301
BaeI ACNNNNGTAYC 2 cut(s) 280, 313
BanI GGYRCC 2 cut(s) 296, 978
BauI CACGAG 1 cut(s) 1071
BbsI GAAGAC 1 cut(s) 840
BccI CCATC 3 cut(s) 706, 900, 1148
BciT130I CCWGG 2 cut(s) 529, 724
BciVI GTATCC 1 cut(s) 643
BcoDI GTCTC 5 cut(s) 13, 674, 888, 1247, 1266
BfaI CTAG 1 cut(s) 926
BfmI CTRYAG 3 cut(s) 317, 469, 754
BfuI GTATCC 1 cut(s) 643
BglII AGATCT 1 cut(s) 43
BmcAI AGTACT 1 cut(s) 1059
Bme1390I CCNGG 2 cut(s) 529, 724
BmeT110I CYCGRG 1 cut(s) 885
BmiI GGNNCC 3 cut(s) 298, 695, 980
BmrFI CCNGG 2 cut(s) 529, 724
BmrI ACTGGG 1 cut(s) 838
BmsI GCATC 2 cut(s) 180, 907
BmuI ACTGGG 1 cut(s) 838
BpiI GAAGAC 1 cut(s) 840
BplI GAGNNNNNCTC 4 cut(s) 996, 1028, 1054, 1086
BpmI CTGGAG 1 cut(s) 184
BsaBI GATNNNNATC 1 cut(s) 311
BsaHI GRCGYC 1 cut(s) 1236
BsaI GGTCTC 1 cut(s) 674
BsaJI CCNNGG 5 cut(s) 21, 292, 302, 722, 1015
BsaWI WCCGGW 1 cut(s) 220
BsaXI ACNNNNNCTCC 3 cut(s) 259, 289, 1246
Bsc4I CCNNNNNNNGG 3 cut(s) 114, 528, 690
Bse1I ACTGG 2 cut(s) 776, 844
Bse3DI GCAATG 1 cut(s) 27
Bse8I GATNNNNATC 1 cut(s) 311
BseAI TCCGGA 1 cut(s) 220
BseBI CCWGG 2 cut(s) 529, 724
BseDI CCNNGG 5 cut(s) 21, 292, 302, 722, 1015
BseGI GGATG 2 cut(s) 156, 421
BseJI GATNNNNATC 1 cut(s) 311
BseLI CCNNNNNNNGG 3 cut(s) 114, 528, 690
BseMI GCAATG 1 cut(s) 27
BseMII CTCAG 3 cut(s) 275, 669, 1052
BseNI ACTGG 2 cut(s) 776, 844
BseRI GAGGAG 1 cut(s) 1208
BseSI GKGCMC 1 cut(s) 301
BseYI CCCAGC 1 cut(s) 783
BshFI GGCC 3 cut(s) 189, 353, 891
BshNI GGYRCC 2 cut(s) 296, 978
BsiHKCI CYCGRG 1 cut(s) 885
BsiSI CCGG 1 cut(s) 221
BslFI GGGAC 1 cut(s) 1030
BslI CCNNNNNNNGG 3 cut(s) 114, 528, 690
BsmAI GTCTC 5 cut(s) 13, 674, 888, 1247, 1266
BsmFI GGGAC 1 cut(s) 1030
BsmI GAATGC 1 cut(s) 604
BsnI GGCC 3 cut(s) 189, 353, 891
Bso31I GGTCTC 1 cut(s) 674
BsoBI CYCGRG 1 cut(s) 885
Bsp1286I GDGCHC 1 cut(s) 301
Bsp13I TCCGGA 1 cut(s) 220
Bsp143I GATC 5 cut(s) 43, 306, 312, 367, 595
BspACI CCGC 2 cut(s) 160, 1100
BspANI GGCC 3 cut(s) 189, 353, 891
BspCNI CTCAG 3 cut(s) 276, 670, 1053
BspEI TCCGGA 1 cut(s) 220
BspLI GGNNCC 3 cut(s) 298, 695, 980
BspMAI CTGCAG 2 cut(s) 473, 758
BspPI GGATC 4 cut(s) 307, 314, 362, 590
BspT107I GGYRCC 2 cut(s) 296, 978
BspTNI GGTCTC 1 cut(s) 674
BsrDI GCAATG 1 cut(s) 27
BsrI ACTGG 2 cut(s) 776, 844
BssECI CCNNGG 5 cut(s) 21, 292, 302, 722, 1015
BssMI GATC 5 cut(s) 43, 306, 312, 367, 595
BssNI GRCGYC 1 cut(s) 1236
BssSI CACGAG 1 cut(s) 1071
BssT1I CCWWGG 1 cut(s) 292
Bst2BI CACGAG 1 cut(s) 1071
Bst2UI CCWGG 2 cut(s) 529, 724
Bst4CI ACNGT 4 cut(s) 114, 321, 612, 865
BstACI GRCGYC 1 cut(s) 1236
BstC8I GCNNGC 2 cut(s) 758, 762
BstDEI CTNAG 3 cut(s) 284, 678, 1061
BstDSI CCRYGG 2 cut(s) 21, 1015
BstF5I GGATG 2 cut(s) 156, 421
BstKTI GATC 5 cut(s) 46, 309, 315, 370, 598
BstMAI GTCTC 5 cut(s) 13, 674, 888, 1247, 1266
BstMBI GATC 5 cut(s) 43, 306, 312, 367, 595
BstMWI GCNNNNNNNGC 2 cut(s) 168, 1035
BstNI CCWGG 2 cut(s) 529, 724
BstNSI RCATGY 1 cut(s) 446
BstSCI CCNGG 2 cut(s) 527, 722
BstSFI CTRYAG 3 cut(s) 317, 469, 754
BstSLI GKGCMC 1 cut(s) 301
BstV2I GAAGAC 1 cut(s) 840
BstX2I RGATCY 2 cut(s) 43, 312
BstXI CCANNNNNNTGG 2 cut(s) 438, 494
BstYI RGATCY 2 cut(s) 43, 312
BsuI GTATCC 1 cut(s) 643
BsuRI GGCC 3 cut(s) 189, 353, 891
BtgI CCRYGG 2 cut(s) 21, 1015
BtgZI GCGATG 1 cut(s) 157
BtsCI GGATG 2 cut(s) 156, 421
BtsI GCAGTG 1 cut(s) 267
BtsIMutI CAGTG 4 cut(s) 119, 267, 769, 978
Cac8I GCNNGC 2 cut(s) 758, 762
CseI GACGC 1 cut(s) 1244
Csp6I GTAC 5 cut(s) 565, 613, 632, 979, 1058
CspCI CAANNNNNGTGG 2 cut(s) 10, 45
CviAII CATG 1 cut(s) 443
CviQI GTAC 5 cut(s) 565, 613, 632, 979, 1058
DdeI CTNAG 3 cut(s) 284, 678, 1061
DpnI GATC 5 cut(s) 45, 308, 314, 369, 597
DpnII GATC 5 cut(s) 43, 306, 312, 367, 595
DraI TTTAAA 1 cut(s) 1212
DraIII CACNNNGTG 1 cut(s) 1016
Eco130I CCWWGG 1 cut(s) 292
Eco31I GGTCTC 1 cut(s) 674
Eco57I CTGAAG 1 cut(s) 1038
Eco88I CYCGRG 1 cut(s) 885
EcoRI GAATTC 1 cut(s) 140
EcoRII CCWGG 2 cut(s) 527, 722
EcoT14I CCWWGG 1 cut(s) 292
ErhI CCWWGG 1 cut(s) 292
FaeI CATG 1 cut(s) 446
FaqI GGGAC 1 cut(s) 1030
FatI CATG 1 cut(s) 442
FokI GGATG 2 cut(s) 143, 428
FspBI CTAG 1 cut(s) 926
GsaI CCCAGC 1 cut(s) 787
GsuI CTGGAG 1 cut(s) 184
HaeIII GGCC 3 cut(s) 189, 353, 891
HapII CCGG 1 cut(s) 221
HgaI GACGC 1 cut(s) 1244
Hin1I GRCGYC 1 cut(s) 1236
Hin1II CATG 1 cut(s) 446
HincII GTYRAC 1 cut(s) 912
HindII GTYRAC 1 cut(s) 912
HindIII AAGCTT 1 cut(s) 1160
HinfI GANTC 6 cut(s) 483, 504, 643, 881, 1091, 1255
HpaII CCGG 1 cut(s) 221
HphI GGTGA 3 cut(s) 142, 600, 656
Hpy166II GTNNAC 5 cut(s) 632, 743, 811, 912, 1204
Hpy188I TCNGA 9 cut(s) 243, 262, 285, 306, 394, 503, 639, 679, 1096
Hpy188III TCNNGA 8 cut(s) 221, 310, 538, 647, 885, 926, 992, 1205
Hpy8I GTNNAC 5 cut(s) 632, 743, 811, 912, 1204
HpyAV CCTTC 3 cut(s) 192, 1079, 1197
HpyCH4III ACNGT 4 cut(s) 114, 321, 612, 865
HpyCH4IV ACGT 1 cut(s) 510
HpyCH4V TGCA 8 cut(s) 214, 471, 516, 604, 719, 756, 898, 1143
HpyF10VI GCNNNNNNNGC 2 cut(s) 168, 1035
HpyF3I CTNAG 3 cut(s) 284, 678, 1061
HpySE526I ACGT 1 cut(s) 510
Hsp92I GRCGYC 1 cut(s) 1236
Hsp92II CATG 1 cut(s) 446
Kpn2I TCCGGA 1 cut(s) 220
KpnI GGTACC 1 cut(s) 982
Kzo9I GATC 5 cut(s) 43, 306, 312, 367, 595
LmnI GCTCC 1 cut(s) 1221
LweI GCATC 2 cut(s) 180, 907
MaeI CTAG 1 cut(s) 926
MaeII ACGT 1 cut(s) 510
MaeIII GTNAC 2 cut(s) 148, 1066
MalI GATC 5 cut(s) 45, 308, 314, 369, 597
MboI GATC 5 cut(s) 43, 306, 312, 367, 595
MboII GAAGA 6 cut(s) 38, 53, 339, 652, 693, 840
MflI RGATCY 2 cut(s) 43, 312
MhlI GDGCHC 1 cut(s) 301
MluCI AATT 7 cut(s) 140, 373, 533, 673, 765, 868, 1144
MlyI GAGTC 2 cut(s) 890, 1249
MmeI TCCRAC 1 cut(s) 927
MroI TCCGGA 1 cut(s) 220
MroXI GAANNNNTTC 2 cut(s) 45, 1200
MseI TTAA 4 cut(s) 197, 714, 1164, 1211
MspA1I CMGCKG 1 cut(s) 162
MspI CCGG 1 cut(s) 221
MspR9I CCNGG 2 cut(s) 529, 724
Mva1269I GAATGC 1 cut(s) 604
MvaI CCWGG 2 cut(s) 529, 724
MwoI GCNNNNNNNGC 2 cut(s) 168, 1035
NdeII GATC 5 cut(s) 43, 306, 312, 367, 595
NlaIII CATG 1 cut(s) 446
NlaIV GGNNCC 3 cut(s) 298, 695, 980
NmuCI GTSAC 1 cut(s) 148
NspI RCATGY 1 cut(s) 446
PaeR7I CTCGAG 1 cut(s) 885
PctI GAATGC 1 cut(s) 604
PdmI GAANNNNTTC 2 cut(s) 45, 1200
PfeI GAWTC 4 cut(s) 483, 504, 643, 1091
PflFI GACNNNGTC 1 cut(s) 1258
PflMI CCANNNNNTGG 2 cut(s) 114, 690
PleI GAGTC 2 cut(s) 889, 1249
PpsI GAGTC 2 cut(s) 889, 1249
Psp6I CCWGG 2 cut(s) 527, 722
PspFI CCCAGC 1 cut(s) 783
PspGI CCWGG 2 cut(s) 527, 722
PspN4I GGNNCC 3 cut(s) 298, 695, 980
PstI CTGCAG 2 cut(s) 473, 758
PsuI RGATCY 2 cut(s) 43, 312
PsyI GACNNNGTC 1 cut(s) 1258
RsaI GTAC 5 cut(s) 566, 614, 633, 980, 1059
RsaNI GTAC 5 cut(s) 565, 613, 632, 979, 1058
SaqAI TTAA 4 cut(s) 197, 714, 1164, 1211
Sau3AI GATC 5 cut(s) 43, 306, 312, 367, 595
ScaI AGTACT 1 cut(s) 1059
SchI GAGTC 2 cut(s) 890, 1249
ScrFI CCNGG 2 cut(s) 529, 724
SduI GDGCHC 1 cut(s) 301
SfaNI GCATC 2 cut(s) 180, 907
SfcI CTRYAG 3 cut(s) 317, 469, 754
Sfr274I CTCGAG 1 cut(s) 885
SlaI CTCGAG 1 cut(s) 885
SmlI CTYRAG 1 cut(s) 885
SmoI CTYRAG 1 cut(s) 885
Sse9I AATT 7 cut(s) 140, 373, 533, 673, 765, 868, 1144
SsiI CCGC 2 cut(s) 160, 1100
SspMI CTAG 1 cut(s) 926
StyD4I CCNGG 2 cut(s) 527, 722
StyI CCWWGG 1 cut(s) 292
TaaI ACNGT 4 cut(s) 114, 321, 612, 865
TaiI ACGT 1 cut(s) 513
TaqI TCGA 4 cut(s) 67, 851, 886, 933
TasI AATT 7 cut(s) 140, 373, 533, 673, 765, 868, 1144
TatI WGTACW 2 cut(s) 631, 1057
TfiI GAWTC 4 cut(s) 483, 504, 643, 1091
Tru1I TTAA 4 cut(s) 197, 714, 1164, 1211
Tru9I TTAA 4 cut(s) 197, 714, 1164, 1211
TscAI CASTG 4 cut(s) 119, 274, 776, 978
TseFI GTSAC 1 cut(s) 148
Tsp45I GTSAC 1 cut(s) 148
TspDTI ATGAA 3 cut(s) 133, 393, 1237
TspGWI ACGGA 3 cut(s) 38, 728, 1004
TspRI CASTG 4 cut(s) 119, 274, 776, 978
Tth111I GACNNNGTC 1 cut(s) 1258
Van91I CCANNNNNTGG 2 cut(s) 114, 690
XapI RAATTY 1 cut(s) 140
XbaI TCTAGA 1 cut(s) 925
XceI RCATGY 1 cut(s) 446
XhoI CTCGAG 1 cut(s) 885
XmnI GAANNNNTTC 2 cut(s) 45, 1200
XspI CTAG 1 cut(s) 926
ZrmI AGTACT 1 cut(s) 1059
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.