Rh4CG089400

F-box domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0014709
Physical Location & Seq
Reverse (-)
16047679 .. 16048950
1272 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG089400.1

Sequence Viewer

Length: 1272 bp
ATGTCAGTCTCATCATCAGCCACAGACATTGCCAACAACGAAGATCTTCTATCCCAAATCCTTGTTCGATTACCCCCAAAATCCCTCCTTTGCTTCAAATGTGTGTCCAAACAGTGGCTTTCTCTCATTTCCAGCCCCGGATTCATTGTCACCCACATCCGCTGGAGAGGCATCACTTCCCCTTCTGGCCTACTCTTAAACAATGATTACTTGCACACTCCGGAGTTCCACTATGTTCCTCTGACACACAATAATCCCTCTGAACTCACTGCCCCTCCATATCTGAGCTACCTTGGTGCCCCTCGGATCAAGATCATACAGTCTTGTAATGGTTTGCTCCTGTGTTCTTCGGCCTACCTAAAAAATGATCCAAATTATGAACTCAAATACTCTGATGACTTCACGCAGACAGAGGATGATGTTCTCTTGCCAGATATATGGCATGTTATCCCAACAATCTCAAACGCCTGCAGTCAAACAAGAATCCATTATTCTGGTTTCAGATTCTACGTCTGCAATCCCACCACCAGGCAATTCAAGACCCTTTCTTTTCCTTTCTTTCAGTACCGCATACTTGCTCTAAATCTGGCTTTTGATCCTTTGCATTCACCGTACTTCAAAATAGTGTGTGTACTTCGGAAGAATCTGGATACTTATATACTACACCTATATAATTCTGAGACCAAAACTTGGAACCCATCTTCAATATCTTTTAATGCACCCAGGACAATATACTTCCATGAACCTGTGTTCTGCAGGCGAGCAATTCACTGGTATAGTTCCCAGCAGACTTCTCTTTACTTTGATGTGGACAAAGAGTGCTTGAACCCAATGCCTATGCCCCAGTCTTTGATATATGGAACTGTCAATTATTTTGGGGAGTCTCGAGGCCATCTGCATCTGGTAATGTCAACAACCTTTATTCTAGAACTCGATGTGTTTGAGTTGGAGAAAGATTACTCTGTATGGTCAGTGAGGTACCATATCAATCTTGACACCATAAGAGCTACACTCCGTGGTATGTGTGGCTTTTCCAGCGACCTGTCCCTTGCTTCAGTACTGAGAGTTACTCGTGGAGAAAAGGAAGGGGAATCAGAGGCGGTGGTATTTATGTTTGGTATGGAATGTATAGTTGTATCCTGCAATTTGAGTGATGGGACGCTTAAAAGACATATGGGTTTTGTTCCGTCTCGGGAATCTGTTCACGATTTAAAGCTCCGCCATTCCTATACAGACGCCTTTCCATTCATAGAGACTCTGTCTCCCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

423

Amino Acids

48.49

Weight (kDa)

6.59

Isoelectric Point (pI)

51.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 14 - 48 6.3e-08 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g06280 FvH4_4g06280 FvH4_4g06280 FvH4_4g06280
malus_domestica MD16G1239800.v1.1
rosa_chinensis RchiOBHm_Chr4g0398001
rosa_laevigata RLG00000009391
rosa_multiflora Rmu_sc0003773.1_g000024
rosa_roxburghii Rroxscaffold_5G00342960
rosa_rugosa Rorug04G0005200 Rorug04G0005300
rosa_samantha Rh4AG081900 Rh4BG079600 Rh4CG089400 Rh4DG075000
rosa_wichuraiana Rw0G019320 Rw4G006830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 978
AccB1I GGYRCC 2 cut(s) 296, 978
AccB7I CCANNNNNTGG 2 cut(s) 114, 690
AccIII TCCGGA 1 cut(s) 220
AciI CCGC 4 cut(s) 160, 568, 1100, 1219
AclWI GGATC 3 cut(s) 314, 362, 590
AcuI CTGAAG 1 cut(s) 1038
AcyI GRCGYC 1 cut(s) 1236
AdeI CACNNNGTG 1 cut(s) 1016
AfaI GTAC 5 cut(s) 566, 614, 633, 980, 1059
AfiI CCNNNNNNNGG 3 cut(s) 114, 528, 690
AgsI TTSAA 5 cut(s) 97, 538, 619, 705, 826
AjnI CCWGG 2 cut(s) 527, 722
AjuI GAANNNNNNNTTGG 2 cut(s) 48, 80
AluBI AGCT 3 cut(s) 288, 1007, 1216
AluI AGCT 3 cut(s) 288, 1007, 1216
Alw26I GTCTC 6 cut(s) 13, 674, 888, 1194, 1247, 1266
AlwI GGATC 3 cut(s) 314, 362, 590
Ama87I CYCGRG 2 cut(s) 885, 1191
Aor13HI TCCGGA 1 cut(s) 220
AoxI GGCC 3 cut(s) 187, 351, 889
Asp700I GAANNNNTTC 2 cut(s) 45, 1200
Asp718I GGTACC 1 cut(s) 978
AsuC2I CCSGG 1 cut(s) 138
AsuHPI GGTGA 2 cut(s) 142, 600
AvaI CYCGRG 2 cut(s) 885, 1191
BaeGI GKGCMC 1 cut(s) 301
BanI GGYRCC 2 cut(s) 296, 978
BauI CACGAG 1 cut(s) 1071
BccI CCATC 3 cut(s) 706, 900, 1148
BciT130I CCWGG 2 cut(s) 529, 724
BciVI GTATCC 2 cut(s) 643, 1147
BcnI CCSGG 1 cut(s) 138
BcoDI GTCTC 6 cut(s) 13, 674, 888, 1194, 1247, 1266
BfaI CTAG 1 cut(s) 926
BfmI CTRYAG 2 cut(s) 469, 754
BfuI GTATCC 2 cut(s) 643, 1147
BglII AGATCT 1 cut(s) 43
BmcAI AGTACT 1 cut(s) 1059
Bme1390I CCNGG 3 cut(s) 138, 529, 724
BmeT110I CYCGRG 2 cut(s) 885, 1191
BmiI GGNNCC 3 cut(s) 298, 695, 980
BmrFI CCNGG 3 cut(s) 138, 529, 724
BmrI ACTGGG 1 cut(s) 838
BmsI GCATC 2 cut(s) 180, 907
BmuI ACTGGG 1 cut(s) 838
BplI GAGNNNNNCTC 4 cut(s) 996, 1028, 1054, 1086
BpmI CTGGAG 1 cut(s) 184
BpuMI CCSGG 1 cut(s) 138
BsaBI GATNNNNATC 1 cut(s) 311
BsaHI GRCGYC 1 cut(s) 1236
BsaI GGTCTC 1 cut(s) 674
BsaJI CCNNGG 5 cut(s) 136, 292, 302, 722, 1015
BsaWI WCCGGW 1 cut(s) 220
BsaXI ACNNNNNCTCC 3 cut(s) 259, 289, 1246
Bsc4I CCNNNNNNNGG 3 cut(s) 114, 528, 690
Bse1I ACTGG 2 cut(s) 776, 844
Bse3DI GCAATG 1 cut(s) 27
Bse8I GATNNNNATC 1 cut(s) 311
BseAI TCCGGA 1 cut(s) 220
BseBI CCWGG 2 cut(s) 529, 724
BseDI CCNNGG 5 cut(s) 136, 292, 302, 722, 1015
BseGI GGATG 2 cut(s) 156, 421
BseJI GATNNNNATC 1 cut(s) 311
BseLI CCNNNNNNNGG 3 cut(s) 114, 528, 690
BseMI GCAATG 1 cut(s) 27
BseMII CTCAG 3 cut(s) 275, 669, 1052
BseNI ACTGG 2 cut(s) 776, 844
BseSI GKGCMC 1 cut(s) 301
BseYI CCCAGC 1 cut(s) 783
BshFI GGCC 3 cut(s) 189, 353, 891
BshNI GGYRCC 2 cut(s) 296, 978
BsiHKCI CYCGRG 2 cut(s) 885, 1191
BsiSI CCGG 2 cut(s) 138, 221
BslFI GGGAC 2 cut(s) 1030, 1171
BslI CCNNNNNNNGG 3 cut(s) 114, 528, 690
BsmAI GTCTC 6 cut(s) 13, 674, 888, 1194, 1247, 1266
BsmBI CGTCTC 1 cut(s) 1194
BsmFI GGGAC 2 cut(s) 1030, 1171
BsmI GAATGC 1 cut(s) 604
BsnI GGCC 3 cut(s) 189, 353, 891
Bso31I GGTCTC 1 cut(s) 674
BsoBI CYCGRG 2 cut(s) 885, 1191
Bsp1286I GDGCHC 1 cut(s) 301
Bsp13I TCCGGA 1 cut(s) 220
Bsp143I GATC 5 cut(s) 43, 306, 312, 367, 595
BspACI CCGC 4 cut(s) 160, 568, 1100, 1219
BspANI GGCC 3 cut(s) 189, 353, 891
BspCNI CTCAG 3 cut(s) 276, 670, 1053
BspEI TCCGGA 1 cut(s) 220
BspLI GGNNCC 3 cut(s) 298, 695, 980
BspMAI CTGCAG 2 cut(s) 473, 758
BspPI GGATC 3 cut(s) 314, 362, 590
BspT107I GGYRCC 2 cut(s) 296, 978
BspTNI GGTCTC 1 cut(s) 674
BsrDI GCAATG 1 cut(s) 27
BsrI ACTGG 2 cut(s) 776, 844
BssECI CCNNGG 5 cut(s) 136, 292, 302, 722, 1015
BssMI GATC 5 cut(s) 43, 306, 312, 367, 595
BssNI GRCGYC 1 cut(s) 1236
BssSI CACGAG 1 cut(s) 1071
BssT1I CCWWGG 1 cut(s) 292
Bst2BI CACGAG 1 cut(s) 1071
Bst2UI CCWGG 2 cut(s) 529, 724
Bst4CI ACNGT 4 cut(s) 114, 321, 612, 865
BstACI GRCGYC 1 cut(s) 1236
BstC8I GCNNGC 3 cut(s) 469, 758, 762
BstDEI CTNAG 3 cut(s) 284, 678, 1061
BstDSI CCRYGG 1 cut(s) 1015
BstF5I GGATG 2 cut(s) 156, 421
BstKTI GATC 5 cut(s) 46, 309, 315, 370, 598
BstMAI GTCTC 6 cut(s) 13, 674, 888, 1194, 1247, 1266
BstMBI GATC 5 cut(s) 43, 306, 312, 367, 595
BstMWI GCNNNNNNNGC 2 cut(s) 168, 1035
BstNI CCWGG 2 cut(s) 529, 724
BstNSI RCATGY 1 cut(s) 446
BstSCI CCNGG 3 cut(s) 136, 527, 722
BstSFI CTRYAG 2 cut(s) 469, 754
BstSLI GKGCMC 1 cut(s) 301
BstX2I RGATCY 1 cut(s) 43
BstXI CCANNNNNNTGG 2 cut(s) 438, 494
BstYI RGATCY 1 cut(s) 43
BsuI GTATCC 2 cut(s) 643, 1147
BsuRI GGCC 3 cut(s) 189, 353, 891
BtgI CCRYGG 1 cut(s) 1015
BtsCI GGATG 2 cut(s) 156, 421
BtsI GCAGTG 1 cut(s) 267
BtsIMutI CAGTG 4 cut(s) 119, 267, 769, 978
Cac8I GCNNGC 3 cut(s) 469, 758, 762
CseI GACGC 2 cut(s) 1168, 1244
Csp6I GTAC 5 cut(s) 565, 613, 632, 979, 1058
CspCI CAANNNNNGTGG 2 cut(s) 10, 45
CviAII CATG 2 cut(s) 443, 740
CviQI GTAC 5 cut(s) 565, 613, 632, 979, 1058
DdeI CTNAG 3 cut(s) 284, 678, 1061
DpnI GATC 5 cut(s) 45, 308, 314, 369, 597
DpnII GATC 5 cut(s) 43, 306, 312, 367, 595
DraI TTTAAA 1 cut(s) 1212
DraIII CACNNNGTG 1 cut(s) 1016
EciI GGCGGA 1 cut(s) 1208
Eco130I CCWWGG 1 cut(s) 292
Eco31I GGTCTC 1 cut(s) 674
Eco57I CTGAAG 1 cut(s) 1038
Eco88I CYCGRG 2 cut(s) 885, 1191
EcoRII CCWGG 2 cut(s) 527, 722
EcoT14I CCWWGG 1 cut(s) 292
ErhI CCWWGG 1 cut(s) 292
Esp3I CGTCTC 1 cut(s) 1194
FaeI CATG 2 cut(s) 446, 743
FaqI GGGAC 2 cut(s) 1030, 1171
FatI CATG 2 cut(s) 442, 739
FauNDI CATATG 1 cut(s) 1173
FokI GGATG 2 cut(s) 143, 428
FspBI CTAG 1 cut(s) 926
GsaI CCCAGC 1 cut(s) 787
GsuI CTGGAG 1 cut(s) 184
HaeIII GGCC 3 cut(s) 189, 353, 891
HapII CCGG 2 cut(s) 138, 221
HgaI GACGC 2 cut(s) 1168, 1244
Hin1I GRCGYC 1 cut(s) 1236
Hin1II CATG 2 cut(s) 446, 743
HincII GTYRAC 1 cut(s) 912
HindII GTYRAC 1 cut(s) 912
HinfI GANTC 8 cut(s) 141, 483, 504, 643, 881, 1091, 1196, 1255
HpaII CCGG 2 cut(s) 138, 221
HphI GGTGA 2 cut(s) 142, 600
Hpy166II GTNNAC 4 cut(s) 632, 811, 912, 1204
Hpy188I TCNGA 9 cut(s) 243, 262, 285, 306, 394, 503, 639, 679, 1096
Hpy188III TCNNGA 9 cut(s) 221, 310, 538, 647, 885, 926, 992, 1193, 1205
Hpy8I GTNNAC 4 cut(s) 632, 811, 912, 1204
HpyAV CCTTC 2 cut(s) 192, 1079
HpyCH4III ACNGT 4 cut(s) 114, 321, 612, 865
HpyCH4IV ACGT 1 cut(s) 510
HpyCH4V TGCA 8 cut(s) 214, 471, 516, 604, 719, 756, 898, 1143
HpyF10VI GCNNNNNNNGC 2 cut(s) 168, 1035
HpyF3I CTNAG 3 cut(s) 284, 678, 1061
HpySE526I ACGT 1 cut(s) 510
Hsp92I GRCGYC 1 cut(s) 1236
Hsp92II CATG 2 cut(s) 446, 743
Kpn2I TCCGGA 1 cut(s) 220
KpnI GGTACC 1 cut(s) 982
Kzo9I GATC 5 cut(s) 43, 306, 312, 367, 595
LmnI GCTCC 2 cut(s) 342, 1221
LweI GCATC 2 cut(s) 180, 907
MaeI CTAG 1 cut(s) 926
MaeII ACGT 1 cut(s) 510
MaeIII GTNAC 2 cut(s) 148, 1066
MalI GATC 5 cut(s) 45, 308, 314, 369, 597
MboI GATC 5 cut(s) 43, 306, 312, 367, 595
MboII GAAGA 5 cut(s) 38, 53, 339, 652, 693
MflI RGATCY 1 cut(s) 43
MhlI GDGCHC 1 cut(s) 301
MluCI AATT 6 cut(s) 373, 533, 673, 765, 868, 1144
MlyI GAGTC 2 cut(s) 890, 1249
MmeI TCCRAC 1 cut(s) 927
MroI TCCGGA 1 cut(s) 220
MroXI GAANNNNTTC 2 cut(s) 45, 1200
MseI TTAA 4 cut(s) 197, 714, 1164, 1211
MspA1I CMGCKG 1 cut(s) 162
MspI CCGG 2 cut(s) 138, 221
MspR9I CCNGG 3 cut(s) 138, 529, 724
Mva1269I GAATGC 1 cut(s) 604
MvaI CCWGG 2 cut(s) 529, 724
MwoI GCNNNNNNNGC 2 cut(s) 168, 1035
NciI CCSGG 1 cut(s) 138
NdeI CATATG 1 cut(s) 1173
NdeII GATC 5 cut(s) 43, 306, 312, 367, 595
NlaIII CATG 2 cut(s) 446, 743
NlaIV GGNNCC 3 cut(s) 298, 695, 980
NmuCI GTSAC 1 cut(s) 148
NspI RCATGY 1 cut(s) 446
PaeR7I CTCGAG 1 cut(s) 885
PctI GAATGC 1 cut(s) 604
PdmI GAANNNNTTC 2 cut(s) 45, 1200
PfeI GAWTC 6 cut(s) 141, 483, 504, 643, 1091, 1196
PflFI GACNNNGTC 1 cut(s) 1258
PflMI CCANNNNNTGG 2 cut(s) 114, 690
PleI GAGTC 2 cut(s) 889, 1249
PpsI GAGTC 2 cut(s) 889, 1249
Psp6I CCWGG 2 cut(s) 527, 722
PspFI CCCAGC 1 cut(s) 783
PspGI CCWGG 2 cut(s) 527, 722
PspN4I GGNNCC 3 cut(s) 298, 695, 980
PstI CTGCAG 2 cut(s) 473, 758
PsuI RGATCY 1 cut(s) 43
PsyI GACNNNGTC 1 cut(s) 1258
RsaI GTAC 5 cut(s) 566, 614, 633, 980, 1059
RsaNI GTAC 5 cut(s) 565, 613, 632, 979, 1058
SaqAI TTAA 4 cut(s) 197, 714, 1164, 1211
Sau3AI GATC 5 cut(s) 43, 306, 312, 367, 595
ScaI AGTACT 1 cut(s) 1059
SchI GAGTC 2 cut(s) 890, 1249
ScrFI CCNGG 3 cut(s) 138, 529, 724
SduI GDGCHC 1 cut(s) 301
SfaNI GCATC 2 cut(s) 180, 907
SfcI CTRYAG 2 cut(s) 469, 754
Sfr274I CTCGAG 1 cut(s) 885
SlaI CTCGAG 1 cut(s) 885
SmlI CTYRAG 1 cut(s) 885
SmoI CTYRAG 1 cut(s) 885
Sse9I AATT 6 cut(s) 373, 533, 673, 765, 868, 1144
SsiI CCGC 4 cut(s) 160, 568, 1100, 1219
SspMI CTAG 1 cut(s) 926
StyD4I CCNGG 3 cut(s) 136, 527, 722
StyI CCWWGG 1 cut(s) 292
TaaI ACNGT 4 cut(s) 114, 321, 612, 865
TaiI ACGT 1 cut(s) 513
TaqI TCGA 3 cut(s) 67, 886, 933
TasI AATT 6 cut(s) 373, 533, 673, 765, 868, 1144
TatI WGTACW 2 cut(s) 631, 1057
TfiI GAWTC 6 cut(s) 141, 483, 504, 643, 1091, 1196
Tru1I TTAA 4 cut(s) 197, 714, 1164, 1211
Tru9I TTAA 4 cut(s) 197, 714, 1164, 1211
TscAI CASTG 4 cut(s) 119, 274, 776, 978
TseFI GTSAC 1 cut(s) 148
Tsp45I GTSAC 1 cut(s) 148
TspDTI ATGAA 4 cut(s) 133, 393, 756, 1237
TspGWI ACGGA 2 cut(s) 1004, 1176
TspRI CASTG 4 cut(s) 119, 274, 776, 978
Tth111I GACNNNGTC 1 cut(s) 1258
Van91I CCANNNNNTGG 2 cut(s) 114, 690
XbaI TCTAGA 1 cut(s) 925
XceI RCATGY 1 cut(s) 446
XhoI CTCGAG 1 cut(s) 885
XmnI GAANNNNTTC 2 cut(s) 45, 1200
XspI CTAG 1 cut(s) 926
ZrmI AGTACT 1 cut(s) 1059
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.