FvH4_4g13300

DNA repair protein RAD50

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0012062
Physical Location & Seq
Reverse (-)
16879375 .. 16892635
13261 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g13300.t1

Sequence Viewer

Length: 3951 bp
ATGAGTACGGTGGACAAGATGCTCATCAAGGGAATCCGGAGCTTCGACCCGGACAACAAACACGTCATCACCTTCTTCAAGCCCCTAACCCTAATCGTCGGCCACAACGGCGCCGGAAAAACCACCATCATCGAGTGCCTCAAGCTTTCCTGCACCGGCGAGATGCCTCCCAACGCCAGGTCCGGCCACAGCTTCATCCACGACCCCAAAGTCGGCGGCGAAACCGAGACCAAGGGCCAGATCAAGCTCCGGTTCAAGACCGCCGCCGGCAAGGACGTCGTGTGCATTAGGTCGTTTCAGCTCACTCAGAAAGTCTCCAAGATGGAGTTTAAAGCCATCGAGAGCGTCCTCCAGACTATTAATCCCCACACTGGAGAGAAAGTTTGCCTTAGCTATAGATGTGCTGACATGGATAAGGAAATCCCTGCTTTAATGGGCGTCTCGAAGGCTATTCTCGAAAATGTGATCTTTGTTCACCAGGACGAAGCCAATTGGCCTTTGCAAGATCCTTCCACCCTGAAGAAAAAGTTTGATGATATCTTCTCTGCCACTCGGTATACCAAGGCATTGGAGGTAATTAAAAAACTTCACAAGGATCAAGCTCAAGAGATAAAGACTTACAAGCTAAAATTGGAGAATCTTCAGACCTTGAAAGATGCTGCATACAAGCTTCGTGAAAGCATTGCTAAAGATGAGGAAAGAACAGAATTAGTGAAAAGACAAATGCAGGAGTTGGAGGGCAGCATTCATGATCTGGATGCTAAAATTCTTAATGCTAGTGAAACACTGAAGCATCTGCAGAACCTGAATGACCAGCTATCTACGAATAAAGCTGTTAGAAGCACTCGACGTGATGAAAAGAGGAGACAGAAAGCAGATCTTCCAGAAAAAAATATAGACACTGATGAAGAGTTGAAGGAATGGAAAACTAAGTTTGATGACAGAATTCGAGATTTAGGAACTCAAATTTTTAAGCTGGAGAGGGAACAGGATGACACAAAGACCAAAAGTAATTTCCTTGGTCAGACAATAAAGGATTCTATATGGGATGTTAGCAAGCTTCAAAATGAAGCTAATGCTTATTTGGACTTAAAGAAACAGAGGGATTCAACCATTCAAAATTTGTTTGCAAGGCACAATTTAGGTTCTCTTCCAGATCCCCCCTTCAACAATGAAGTTGCTATAAACTTGACAGATAGAATAAAATCAAGGTTGCGTGATCTAGAAAAGGATTTGCAAGATAAGAAGAAGTTAAATGAAGCTGAAGTCAAAAAAGCATGGGACCGGTACATGGAATCAAATGATCGTTGGAAACTAAAGGAAGCGCAGCACCAGGCTAAAGCAGAAATAAAGAATGACCTTTTGAAGCGTATCGAGAAGAAAAAAAATGAGCGTGATTCTTTTGAAAGTTCAGTTTCAAACTGTGACCTGTCTCGCATTGATGAGAAAGAAAAAAACATGCGAATTGAGGTTGAGCGAAAGACAAATCAGCTTGCTGTTAGAGATTTTGATTCAGTCATTCGTCAAAAGCAGAGTGAGGTGTTTCGCATTGACCAAGATATTACTGCCGTTACTCGAGAGAAGAATGTCTTAGATGGTGACAGGGATGATAGAGTATTACTGTCTCACAAGAAGACAGACTTGGAGATTCATAAAAGGAAACACAGAAAGATAATTGATGACTACAAGGACAGAATTAGGGGAGTGCTAAAAGGAAGGCTTCCTCCGGACAAGGATTTGAAACATGAAATCACTCAGGCCTTGAGGGTTGTTACCATGGAGTTTGATGACTTGAGCACCAAATCTCGTGAAGTGGAGAAGGAGGTGACTATGTTTCAGATGAAAATACAAGAAGTTAATAATAACCTCTGTAAACACCGTAAAGATCTGGAGTCAAAGAGGAGATATATTGAAACTAGACTTCAGGCCTTAGATCAGCAATCTTTTACTGTTGATTCCTATACCAAAGTCTTGGATTCAGCCAAGGAGAAACGAGATGTGCATAAAAGCAAATACAACTTTGCAGATGGTATGCGACAGATGTTTGATCCTTTTGAAAGAGTGGCTCGTGCTCATCATATATGTCCTTGTTGTGAACGCCCTTTTTCTCCAGACGAGGAAGATGAATTTGTTAAAAAGCAAAAAGTGAAGGCAGCAACTTCTTCTGAGCAAATAAAAGCACTCTTAGTGGAATCCTCAAGTGCTGACTCTTTCTTTCAGCAAGTGGACAAGCTTCGCATGTTTTATGAGGAATATTTAAAAATCGGCAAGGAAGCAATCCCTAATGCTGAGAAAGAACTATCTGAGCTTACTGAAGAGATGGAACAAAAGTCCCAGGCCCTTGATGATGTGTTAGCTGTTTCTGCTCAAGTCAAGTCTGATAAGGATTTAATTGAGGCCTTGGTGCAACCCATTGAAACTGCTGACAGACTACTTCAAGAAATACAAATACGCCAGAAGGAAGTTGATGACTTGGAGTATAAGCTTGATATTCAAGGGCCAGGTGCAAAATCCTTGGAGGATATTCAGTTAGAGCTGACTAATCTGCAAAGCAAAAAGGATAATTTGCATGCAGAACTAGAGAAGTTAAGAGAGGAACAAAGGTACATGGAAGGTGATTTGTCAAGCATTCAAATGCGGTGGCACAGTGTAAGGGAAGAGAAAATGAGGGCAGAAAATATGTTTCATGATTATGAAAGGACAGAAGAAGAGTTAGAGCGTTTGGCCGATGAGAAGAGTCAAATTGAACTCGATGAGAAGCTTTTCGCCGAGGCTCTTGGCCCTTTATCAAGGGAGAAAGATAAATTACTTAATGACTACAATGAAATCAAAGCTGAACTTGGTCGCCAGTGTGAGGAGCAAGCTGAACAAAGCAGAAATTACAAGCAGGAAGTGGATGAACTACTTAAGACTAATTCTAAAATTAAAGAGTACAATGATTTAAAGAAAGGAGAGAGGTTGCAGGAACTGCAAGAGAAGCTACGTCTATCAGAATCTCAACTTCAAAGTTGTGATACTAGATTGCAGGAAATTTCAGCTGAATTAGGAAGAAGTAATAAGTTAATGGAGAGTCAAGAAGAGCTGAGGCGAAATATTGATGCCAATTTGAATTACAGGAAAACCAAAGCTGAAGTACGTCTACTTACTCAAGAGGTCGAATCACTCGAAGCAGAAATCCTACAATTTGGTGAGATATCCAAATTTGAAGCTGAACTTCTAAAGCTCTCACAGGAGAGAGAGAGGCTTCTTTCAGAGTTAAACAAGTTTCAGGGAACAATATCGGTTTACCAGAGCAATATCTCAAATCATAAAGTTGATCTCAAACAGGCACAATACAAGGATGTTGATAAGCGGTACTTTGATCAGCTTATCCAGCTAAAGACGTCTGAGATGGCAAACAAGGACCTGGACAGATACTACAATGCGCTTGACAAAGCACTGATGCGCTTCCACTCAATGAAGATGGAGGAAATAAATAAGATTATAAGGGAGTTGTGGCAGCAGACTTACAGAGGACAAGATATAGATTATATATGTATACATTCAGATTCTGAAAGTGCTGGGACTCGTTCATATAGCTACAAGGTTCTCATGCAGACCGGTGATACAGAGCTAGAAATGAGAGGAAGATGTAGTGCTGGTCAGAAGGTGCTTGCTTCCCTGATCATAAGGTTGGCTTTAGCTGAAACTTTTTGCCTGAATTGTGGAATACTAGCACTAGATGAACCAACTACCAACCTGGACGGACCAAATGCTGAGAGTCTTGCTGCTGCTCTTGTTAGAATTATGGAGGACCGGAAAGGGCAGGAGAACTTTCAGTTGATAGTAATTACTCATGATGAGCGCTTTGCTCAACTAATTGGCCAGCGCCAGCATGCAGAAAAATATTATCGAGTGACAAAGGATGATCACCAGCACAGCATTATTGAAGCCCAAGAGATTTTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000228 GO:0000280 GO:0000722 GO:0000723 GO:0000724 GO:0000725 GO:0000781 GO:0000784 GO:0000785 GO:0000790 GO:0000793 GO:0000794 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003691 GO:0003697 GO:0003824 GO:0004017 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006278 GO:0006281 GO:0006302 GO:0006310 GO:0006312 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007004 GO:0007049 GO:0007127 GO:0007131 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009117 GO:0009123 GO:0009165 GO:0009987 GO:0010833 GO:0016043 GO:0016233 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016776 GO:0018130 GO:0019205 GO:0019438 GO:0019637 GO:0022402 GO:0022414 GO:0030870 GO:0031974 GO:0031981 GO:0032200 GO:0032392 GO:0032508 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0035825 GO:0035861 GO:0042162 GO:0042592 GO:0043047 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0046940 GO:0048285 GO:0050145 GO:0050896 GO:0051276 GO:0051321 GO:0051716 GO:0051880 GO:0055086 GO:0060249 GO:0061982 GO:0065007 GO:0065008 GO:0070013 GO:0070192 GO:0071103 GO:0071704 GO:0071840 GO:0071897 GO:0090304 GO:0090305 GO:0090407 GO:0090734 GO:0097159 GO:0098687 GO:0098847 GO:0140013 GO:1901293 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1903046
KEGG Pathways
Metabolic & Signaling
ko03440 ko03450 ko04218 map03440 map03450 map04218
Pfam Domains
Protein Families
AAA_23 Rad50_zn_hook

Protein Analysis

1317

Amino Acids

153.39

Weight (kDa)

6.0

Isoelectric Point (pI)

44.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SMC_N PF02463 4 - 94 9.8e-06 RecF/RecN/SMC N terminal domain
AAA_23 PF13476 6 - 219 9.9e-26 AAA domain
AAA_15 PF13175 22 - 350 6.1e-09 AAA ATPase domain
Rad50_zn_hook PF04423 688 - 724 7.3e-06 Rad50 zinc hook motif
SMC_N PF02463 1076 - 1302 2.5e-07 RecF/RecN/SMC N terminal domain
SbcC_Walker_B PF13558 1190 - 1259 2.9e-06 SbcC/RAD50-like, Walker B motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012062)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G31970
fragaria_vesca FvH4_4g13300
malus_domestica MD05G1230300.v1.1
prunus_persica Prupe.1G165000_v2.0.a1 Prupe.1G165000_v2.0.a1 Prupe.1G165000_v2.0.a1 Prupe.1G165000_v2.0.a1 Prupe.1G165000_v2.0.a1
pyrus_communis pycom04g01350
rosa_chinensis RchiOBHm_Chr4g0413591
rosa_laevigata RLG00000008210
rosa_multiflora Rmu_sc0014097.1_g000002
rosa_roxburghii Rroxscaffold_5G00357470
rosa_rugosa Rorug04G0121100
rosa_samantha Rh4AG180900 Rh4BG180100 Rh4CG192000 Rh4DG176200
rosa_wichuraiana Rw4G015110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3485
AasI GACNNNNNNGTC 1 cut(s) 209
AatII GACGTC 2 cut(s) 279, 3386
AccB1I GGYRCC 1 cut(s) 110
AccI GTMKAC 3 cut(s) 557, 3139, 3538
AccIII TCCGGA 2 cut(s) 36, 1726
AciI CCGC 5 cut(s) 216, 261, 264, 2638, 3352
AclWI GGATC 4 cut(s) 500, 603, 1151, 2042
AcoI YGGCCR 4 cut(s) 100, 184, 2724, 3862
AcsI RAATTY 7 cut(s) 765, 945, 966, 1120, 2126, 3030, 3200
AcuI CTGAAG 7 cut(s) 539, 626, 809, 1284, 1907, 2334, 3150
AcyI GRCGYC 4 cut(s) 111, 276, 438, 3383
AfaI GTAC 6 cut(s) 7, 1289, 2606, 2933, 3135, 3356
AfeI AGCGCT 1 cut(s) 3845
AfiI CCNNNNNNNGG 5 cut(s) 177, 212, 371, 1291, 2854
AflII CTTAAG 1 cut(s) 2906
AflIII ACRYGT 1 cut(s) 61
AgeI ACCGGT 2 cut(s) 1284, 3599
AjiI CACGTC 2 cut(s) 64, 851
AjnI CCWGG 7 cut(s) 176, 477, 1332, 2334, 2500, 3405, 3738
AjuI GAANNNNNNNTTGG 4 cut(s) 998, 1030, 1625, 1657
Alw21I GWGCWC 2 cut(s) 1799, 2074
Alw26I GTCTC 6 cut(s) 221, 319, 445, 859, 1437, 1629
AlwI GGATC 4 cut(s) 500, 603, 1151, 2042
AlwNI CAGNNNCTG 3 cut(s) 805, 2968, 3551
Ama87I CYCGRG 1 cut(s) 1575
Aor13HI TCCGGA 2 cut(s) 36, 1726
Aor51HI AGCGCT 1 cut(s) 3845
ApeKI GCWGC 7 cut(s) 659, 741, 1327, 2153, 3499, 3767, 3770
ApoI RAATTY 7 cut(s) 765, 945, 966, 1120, 2126, 3030, 3200
AseI ATTAAT 1 cut(s) 360
AsiGI ACCGGT 2 cut(s) 1284, 3599
Asp700I GAANNNNTTC 1 cut(s) 2762
AspLEI GCGC 6 cut(s) 113, 1327, 3427, 3447, 3846, 3870
AspS9I GGNCC 9 cut(s) 180, 235, 1282, 2338, 2498, 2780, 3403, 3746, 3793
AsuC2I CCSGG 1 cut(s) 50
AsuHPI GGTGA 8 cut(s) 61, 467, 1610, 1837, 2627, 3200, 3614, 3902
AvaI CYCGRG 1 cut(s) 1575
AvaII GGWCC 5 cut(s) 180, 1282, 3403, 3746, 3793
BalI TGGCCA 1 cut(s) 3864
BanI GGYRCC 1 cut(s) 110
BarI GAAGNNNNNNTAC 2 cut(s) 3123, 3155
BauI CACGAG 2 cut(s) 1806, 2067
BbsI GAAGAC 1 cut(s) 1640
Bbv12I GWGCWC 2 cut(s) 1799, 2074
BbvCI CCTCAGC 1 cut(s) 3083
BbvI GCAGC 7 cut(s) 646, 753, 1339, 2165, 3511, 3754, 3757
BccI CCATC 8 cut(s) 134, 316, 344, 1589, 2021, 2314, 3385, 3457
BceAI ACGGC 2 cut(s) 124, 1553
BcgI CGANNNNNNTGC 2 cut(s) 259, 293
BciT130I CCWGG 7 cut(s) 178, 479, 1334, 2336, 2502, 3407, 3740
BclI TGATCA 3 cut(s) 3361, 3663, 3907
BcnI CCSGG 1 cut(s) 50
BcoDI GTCTC 6 cut(s) 221, 319, 445, 859, 1437, 1629
BfaI CTAG 8 cut(s) 777, 1223, 1917, 2579, 3018, 3614, 3713, 3719
BfmI CTRYAG 2 cut(s) 394, 797
BfoI RGCGCY 3 cut(s) 114, 3847, 3871
BfrI CTTAAG 1 cut(s) 2906
BglI GCCNNNNNGGC 1 cut(s) 108
BglII AGATCT 2 cut(s) 877, 1885
BisI GCNGC 9 cut(s) 217, 264, 660, 742, 1328, 2154, 3500, 3768, 3771
BlsI GCNGC 9 cut(s) 218, 265, 661, 743, 1329, 2155, 3501, 3769, 3772
Bme1390I CCNGG 8 cut(s) 50, 178, 479, 1334, 2336, 2502, 3407, 3740
Bme18I GGWCC 5 cut(s) 180, 1282, 3403, 3746, 3793
BmeT110I CYCGRG 1 cut(s) 1575
BmgBI CACGTC 2 cut(s) 64, 851
BmgT120I GGNCC 9 cut(s) 180, 235, 1282, 2338, 2498, 2780, 3403, 3746, 3793
BmiI GGNNCC 2 cut(s) 112, 1283
BmrFI CCNGG 8 cut(s) 50, 178, 479, 1334, 2336, 2502, 3407, 3740
BmsI GCATC 7 cut(s) 9, 153, 646, 748, 802, 3088, 3432
BpiI GAAGAC 1 cut(s) 1640
BpmI CTGGAG 5 cut(s) 335, 393, 998, 1910, 2094
Bpu10I CCTNAGC 2 cut(s) 389, 3083
BpuEI CTTGAG 7 cut(s) 125, 588, 1783, 1813, 2182, 2352, 3132
BpuMI CCSGG 1 cut(s) 50
BsaBI GATNNNNATC 1 cut(s) 23
BsaHI GRCGYC 4 cut(s) 111, 276, 438, 3383
BsaI GGTCTC 1 cut(s) 221
BsaJI CCNNGG 9 cut(s) 231, 561, 1018, 1776, 1983, 2334, 2400, 2514, 2769
BsaWI WCCGGW 6 cut(s) 36, 249, 1284, 1726, 3599, 3795
BsaXI ACNNNNNCTCC 2 cut(s) 366, 396
Bsc4I CCNNNNNNNGG 5 cut(s) 177, 212, 371, 1291, 2854
Bse118I RCCGGY 4 cut(s) 155, 266, 1284, 3599
Bse1I ACTGG 2 cut(s) 376, 2848
Bse3DI GCAATG 1 cut(s) 681
Bse8I GATNNNNATC 1 cut(s) 23
BseAI TCCGGA 2 cut(s) 36, 1726
BseBI CCWGG 7 cut(s) 178, 479, 1334, 2336, 2502, 3407, 3740
BseDI CCNNGG 9 cut(s) 231, 561, 1018, 1776, 1983, 2334, 2400, 2514, 2769
BseGI GGATG 8 cut(s) 195, 763, 997, 1054, 1612, 2902, 3346, 3910
BseJI GATNNNNATC 1 cut(s) 23
BseLI CCNNNNNNNGG 5 cut(s) 177, 212, 371, 1291, 2854
BseMI GCAATG 1 cut(s) 681
BseMII CTCAG 8 cut(s) 320, 1769, 2157, 2280, 2295, 3074, 3378, 3747
BseNI ACTGG 2 cut(s) 376, 2848
BseRI GAGGAG 3 cut(s) 877, 1915, 2870
BseXI GCAGC 7 cut(s) 646, 753, 1339, 2165, 3511, 3754, 3757
BseYI CCCAGC 1 cut(s) 3560
BsgI GTGCAG 1 cut(s) 136
BshNI GGYRCC 1 cut(s) 110
BshTI ACCGGT 2 cut(s) 1284, 3599
BsiHKAI GWGCWC 2 cut(s) 1799, 2074
BsiHKCI CYCGRG 1 cut(s) 1575
BslFI GGGAC 3 cut(s) 1295, 2317, 3577
BslI CCNNNNNNNGG 5 cut(s) 177, 212, 371, 1291, 2854
BsmAI GTCTC 6 cut(s) 221, 319, 445, 859, 1437, 1629
BsmBI CGTCTC 1 cut(s) 445
BsmFI GGGAC 3 cut(s) 1295, 2317, 3577
BsmI GAATGC 2 cut(s) 744, 2628
Bso31I GGTCTC 1 cut(s) 221
BsoBI CYCGRG 1 cut(s) 1575
Bsp1286I GDGCHC 2 cut(s) 1799, 2074
Bsp13I TCCGGA 2 cut(s) 36, 1726
Bsp19I CCATGG 1 cut(s) 1776
BspACI CCGC 5 cut(s) 216, 261, 264, 2638, 3352
BspCNI CTCAG 8 cut(s) 319, 1768, 2158, 2281, 2296, 3075, 3379, 3748
BspEI TCCGGA 2 cut(s) 36, 1726
BspHI TCATGA 3 cut(s) 748, 2686, 3835
BspLI GGNNCC 2 cut(s) 112, 1283
BspMAI CTGCAG 1 cut(s) 801
BspPI GGATC 4 cut(s) 500, 603, 1151, 2042
BspQI GCTCTTC 1 cut(s) 3072
BspT107I GGYRCC 1 cut(s) 110
BspTI CTTAAG 1 cut(s) 2906
BspTNI GGTCTC 1 cut(s) 221
BsrDI GCAATG 1 cut(s) 681
BsrFI RCCGGY 4 cut(s) 155, 266, 1284, 3599
BsrI ACTGG 2 cut(s) 376, 2848
BssAI RCCGGY 4 cut(s) 155, 266, 1284, 3599
BssECI CCNNGG 9 cut(s) 231, 561, 1018, 1776, 1983, 2334, 2400, 2514, 2769
BssNAI GTATAC 2 cut(s) 558, 3539
BssNI GRCGYC 4 cut(s) 111, 276, 438, 3383
BssSI CACGAG 2 cut(s) 1806, 2067
BssT1I CCWWGG 7 cut(s) 231, 561, 1018, 1776, 1983, 2400, 2514
Bst1107I GTATAC 2 cut(s) 558, 3539
Bst2BI CACGAG 2 cut(s) 1806, 2067
Bst2UI CCWGG 7 cut(s) 178, 479, 1334, 2336, 2502, 3407, 3740
Bst4CI ACNGT 6 cut(s) 10, 1424, 1623, 1880, 1951, 2648
Bst6I CTCTTC 7 cut(s) 903, 1155, 2310, 2652, 2703, 2729, 3072
BstACI GRCGYC 4 cut(s) 111, 276, 438, 3383
BstAFI CTTAAG 1 cut(s) 2906
BstAPI GCANNNNNTGC 1 cut(s) 2968
BstC8I GCNNGC 9 cut(s) 268, 1058, 1494, 2571, 2862, 3654, 3866, 3872, 3876
BstDSI CCRYGG 1 cut(s) 1776
BstF5I GGATG 8 cut(s) 195, 763, 997, 1054, 1612, 2902, 3346, 3910
BstH2I RGCGCY 3 cut(s) 114, 3847, 3871
BstHHI GCGC 6 cut(s) 113, 1327, 3427, 3447, 3846, 3870
BstMAI GTCTC 6 cut(s) 221, 319, 445, 859, 1437, 1629
BstMWI GCNNNNNNNGC 5 cut(s) 108, 2363, 2968, 2977, 3373
BstNI CCWGG 7 cut(s) 178, 479, 1334, 2336, 2502, 3407, 3740
BstNSI RCATGY 4 cut(s) 1462, 2242, 2573, 3878
BstSCI CCNGG 8 cut(s) 48, 176, 477, 1332, 2334, 2500, 3405, 3738
BstSFI CTRYAG 2 cut(s) 394, 797
BstV1I GCAGC 7 cut(s) 646, 753, 1339, 2165, 3511, 3754, 3757
BstV2I GAAGAC 1 cut(s) 1640
BstX2I RGATCY 4 cut(s) 505, 877, 1156, 1885
BstXI CCANNNNNNTGG 2 cut(s) 568, 1972
BstYI RGATCY 4 cut(s) 505, 877, 1156, 1885
BstZ17I GTATAC 2 cut(s) 558, 3539
BtgI CCRYGG 1 cut(s) 1776
BtrI CACGTC 2 cut(s) 64, 851
BtsCI GGATG 8 cut(s) 195, 763, 997, 1054, 1612, 2902, 3346, 3910
BtsIMutI CAGTG 6 cut(s) 369, 785, 900, 2653, 2855, 3437
Cac8I GCNNGC 9 cut(s) 268, 1058, 1494, 2571, 2862, 3654, 3866, 3872, 3876
CaiI CAGNNNCTG 3 cut(s) 805, 2968, 3551
CciI TCATGA 3 cut(s) 748, 2686, 3835
CfoI GCGC 6 cut(s) 113, 1327, 3427, 3447, 3846, 3870
Cfr10I RCCGGY 4 cut(s) 155, 266, 1284, 3599
Cfr13I GGNCC 9 cut(s) 180, 235, 1282, 2338, 2498, 2780, 3403, 3746, 3793
CseI GACGC 2 cut(s) 334, 427
Csp6I GTAC 6 cut(s) 6, 1288, 2605, 2932, 3134, 3355
CspAI ACCGGT 2 cut(s) 1284, 3599
CspCI CAANNNNNGTGG 2 cut(s) 2621, 2656
CviQI GTAC 6 cut(s) 6, 1288, 2605, 2932, 3134, 3355
DinI GGCGCC 1 cut(s) 112
DraI TTTAAA 3 cut(s) 331, 2259, 2943
DrdI GACNNNNNNGTC 1 cut(s) 209
DseDI GACNNNNNNGTC 1 cut(s) 209
EaeI YGGCCR 4 cut(s) 100, 184, 2724, 3862
Eam1104I CTCTTC 7 cut(s) 903, 1155, 2310, 2652, 2703, 2729, 3072
EarI CTCTTC 7 cut(s) 903, 1155, 2310, 2652, 2703, 2729, 3072
Eco130I CCWWGG 7 cut(s) 231, 561, 1018, 1776, 1983, 2400, 2514
Eco147I AGGCCT 3 cut(s) 1760, 1928, 2399
Eco31I GGTCTC 1 cut(s) 221
Eco32I GATATC 2 cut(s) 538, 3195
Eco47I GGWCC 5 cut(s) 180, 1282, 3403, 3746, 3793
Eco47III AGCGCT 1 cut(s) 3845
Eco57I CTGAAG 7 cut(s) 539, 626, 809, 1284, 1907, 2334, 3150
Eco88I CYCGRG 1 cut(s) 1575
EcoO109I RGGNCCY 2 cut(s) 2338, 3403
EcoRI GAATTC 1 cut(s) 945
EcoRII CCWGG 7 cut(s) 176, 477, 1332, 2334, 2500, 3405, 3738
EcoRV GATATC 2 cut(s) 538, 3195
EcoT14I CCWWGG 7 cut(s) 231, 561, 1018, 1776, 1983, 2400, 2514
EgeI GGCGCC 1 cut(s) 112
EheI GGCGCC 1 cut(s) 112
ErhI CCWWGG 7 cut(s) 231, 561, 1018, 1776, 1983, 2400, 2514
Esp3I CGTCTC 1 cut(s) 445
FaqI GGGAC 3 cut(s) 1295, 2317, 3577
FbaI TGATCA 3 cut(s) 3361, 3663, 3907
FblI GTMKAC 3 cut(s) 557, 3139, 3538
Fnu4HI GCNGC 9 cut(s) 217, 264, 660, 742, 1328, 2154, 3500, 3768, 3771
FokI GGATG 8 cut(s) 182, 770, 1004, 1061, 1619, 2909, 3353, 3917
Fsp4HI GCNGC 9 cut(s) 217, 264, 660, 742, 1328, 2154, 3500, 3768, 3771
FspBI CTAG 8 cut(s) 777, 1223, 1917, 2579, 3018, 3614, 3713, 3719
GlaI GCGC 6 cut(s) 112, 1326, 3426, 3446, 3845, 3869
GluI GCNGC 9 cut(s) 217, 264, 660, 742, 1328, 2154, 3500, 3768, 3771
GsaI CCCAGC 1 cut(s) 3564
GsuI CTGGAG 5 cut(s) 335, 393, 998, 1910, 2094
HaeII RGCGCY 3 cut(s) 114, 3847, 3871
HgaI GACGC 2 cut(s) 334, 427
HhaI GCGC 6 cut(s) 113, 1327, 3427, 3447, 3846, 3870
Hin1I GRCGYC 4 cut(s) 111, 276, 438, 3383
Hin6I GCGC 6 cut(s) 111, 1325, 3425, 3445, 3844, 3868
HinP1I GCGC 6 cut(s) 111, 1325, 3425, 3445, 3844, 3868
HindIII AAGCTT 6 cut(s) 143, 668, 1058, 2231, 2483, 2759
HphI GGTGA 8 cut(s) 61, 467, 1610, 1837, 2627, 3200, 3614, 3902
Hpy166II GTNNAC 9 cut(s) 13, 475, 558, 1874, 2096, 2227, 3140, 3286, 3539
Hpy8I GTNNAC 9 cut(s) 13, 475, 558, 1874, 2096, 2227, 3140, 3286, 3539
Hpy99I CGWCG 3 cut(s) 101, 281, 852
HpyCH4III ACNGT 6 cut(s) 10, 1424, 1623, 1880, 1951, 2648
HpyCH4IV ACGT 6 cut(s) 63, 276, 850, 2983, 3136, 3383
HpyF10VI GCNNNNNNNGC 5 cut(s) 108, 2363, 2968, 2977, 3373
HpySE526I ACGT 6 cut(s) 63, 276, 850, 2983, 3136, 3383
Hsp92I GRCGYC 4 cut(s) 111, 276, 438, 3383
HspAI GCGC 6 cut(s) 111, 1325, 3425, 3445, 3844, 3868
KasI GGCGCC 1 cut(s) 110
Kpn2I TCCGGA 2 cut(s) 36, 1726
KroI GCCGGC 1 cut(s) 266
KroNI GCCGGC 1 cut(s) 268
Ksp22I TGATCA 3 cut(s) 3361, 3663, 3907
LguI GCTCTTC 1 cut(s) 3072
LmnI GCTCC 3 cut(s) 39, 252, 2857
Lsp1109I GCAGC 7 cut(s) 646, 753, 1339, 2165, 3511, 3754, 3757
LweI GCATC 7 cut(s) 9, 153, 646, 748, 802, 3088, 3432
MaeI CTAG 8 cut(s) 777, 1223, 1917, 2579, 3018, 3614, 3713, 3719
MaeII ACGT 6 cut(s) 63, 276, 850, 2983, 3136, 3383
MaeIII GTNAC 6 cut(s) 1424, 1570, 1598, 1771, 1825, 3895
MfeI CAATTG 1 cut(s) 490
MflI RGATCY 4 cut(s) 505, 877, 1156, 1885
MhlI GDGCHC 2 cut(s) 1799, 2074
MlsI TGGCCA 1 cut(s) 3864
MluNI TGGCCA 1 cut(s) 3864
Mly113I GGCGCC 1 cut(s) 111
MlyI GAGTC 6 cut(s) 1901, 2201, 2746, 3079, 3559, 3769
MmeI TCCRAC 2 cut(s) 714, 1289
Mox20I TGGCCA 1 cut(s) 3864
MroI TCCGGA 2 cut(s) 36, 1726
MroNI GCCGGC 1 cut(s) 266
MroXI GAANNNNTTC 1 cut(s) 2762
MscI TGGCCA 1 cut(s) 3864
MslI CAYNNNNRTG 2 cut(s) 2633, 2691
Msp20I TGGCCA 1 cut(s) 3864
MspA1I CMGCKG 1 cut(s) 3038
MspCI CTTAAG 1 cut(s) 2906
MspR9I CCNGG 8 cut(s) 50, 178, 479, 1334, 2336, 2502, 3407, 3740
MunI CAATTG 1 cut(s) 490
Mva1269I GAATGC 2 cut(s) 744, 2628
MvaI CCWGG 7 cut(s) 178, 479, 1334, 2336, 2502, 3407, 3740
MwoI GCNNNNNNNGC 5 cut(s) 108, 2363, 2968, 2977, 3373
NaeI GCCGGC 1 cut(s) 268
NarI GGCGCC 1 cut(s) 111
NciI CCSGG 1 cut(s) 50
NcoI CCATGG 1 cut(s) 1776
NgoMIV GCCGGC 1 cut(s) 266
NlaIV GGNNCC 2 cut(s) 112, 1283
NmeAIII GCCGAG 1 cut(s) 2794
NmuCI GTSAC 4 cut(s) 1424, 1598, 1825, 3895
NspI RCATGY 4 cut(s) 1462, 2242, 2573, 3878
PaeI GCATGC 2 cut(s) 2573, 3878
PaeR7I CTCGAG 1 cut(s) 1575
PagI TCATGA 3 cut(s) 748, 2686, 3835
PceI AGGCCT 3 cut(s) 1760, 1928, 2399
PciSI GCTCTTC 1 cut(s) 3072
PcsI WCGNNNNNNNCGW 1 cut(s) 3162
PctI GAATGC 2 cut(s) 744, 2628
PdiI GCCGGC 1 cut(s) 268
PdmI GAANNNNTTC 1 cut(s) 2762
PinAI ACCGGT 2 cut(s) 1284, 3599
PkrI GCNGC 9 cut(s) 218, 265, 661, 743, 1329, 2155, 3501, 3769, 3772
PleI GAGTC 6 cut(s) 1900, 2201, 2745, 3078, 3559, 3768
PluTI GGCGCC 1 cut(s) 114
PpsI GAGTC 6 cut(s) 1900, 2201, 2745, 3078, 3559, 3768
PpuMI RGGWCCY 1 cut(s) 3403
PshBI ATTAAT 1 cut(s) 360
PsiI TTATAA 1 cut(s) 3485
Psp5II RGGWCCY 1 cut(s) 3403
Psp6I CCWGG 7 cut(s) 176, 477, 1332, 2334, 2500, 3405, 3738
PspFI CCCAGC 1 cut(s) 3560
PspGI CCWGG 7 cut(s) 176, 477, 1332, 2334, 2500, 3405, 3738
PspN4I GGNNCC 2 cut(s) 112, 1283
PspPI GGNCC 9 cut(s) 180, 235, 1282, 2338, 2498, 2780, 3403, 3746, 3793
PspPPI RGGWCCY 1 cut(s) 3403
PstI CTGCAG 1 cut(s) 801
PstNI CAGNNNCTG 3 cut(s) 805, 2968, 3551
PsuI RGATCY 4 cut(s) 505, 877, 1156, 1885
PvuII CAGCTG 1 cut(s) 3038
RsaI GTAC 6 cut(s) 7, 1289, 2606, 2933, 3135, 3356
RsaNI GTAC 6 cut(s) 6, 1288, 2605, 2932, 3134, 3355
RseI CAYNNNNRTG 2 cut(s) 2633, 2691
SapI GCTCTTC 1 cut(s) 3072
SatI GCNGC 9 cut(s) 217, 264, 660, 742, 1328, 2154, 3500, 3768, 3771
Sau96I GGNCC 9 cut(s) 180, 235, 1282, 2338, 2498, 2780, 3403, 3746, 3793
SchI GAGTC 6 cut(s) 1901, 2201, 2746, 3079, 3559, 3769
ScrFI CCNGG 8 cut(s) 50, 178, 479, 1334, 2336, 2502, 3407, 3740
SduI GDGCHC 2 cut(s) 1799, 2074
SfaNI GCATC 7 cut(s) 9, 153, 646, 748, 802, 3088, 3432
SfcI CTRYAG 2 cut(s) 394, 797
SfoI GGCGCC 1 cut(s) 112
Sfr274I CTCGAG 1 cut(s) 1575
SgrAI CRCCGGYG 1 cut(s) 155
SinI GGWCC 5 cut(s) 180, 1282, 3403, 3746, 3793
SlaI CTCGAG 1 cut(s) 1575
SmiMI CAYNNNNRTG 2 cut(s) 2633, 2691
SmlI CTYRAG 9 cut(s) 140, 603, 1575, 1762, 1792, 2197, 2367, 2906, 3147
SmoI CTYRAG 9 cut(s) 140, 603, 1575, 1762, 1792, 2197, 2367, 2906, 3147
SphI GCATGC 2 cut(s) 2573, 3878
SseBI AGGCCT 3 cut(s) 1760, 1928, 2399
SsiI CCGC 5 cut(s) 216, 261, 264, 2638, 3352
SspDI GGCGCC 1 cut(s) 110
SspI AATATT 3 cut(s) 2255, 3094, 3887
SspMI CTAG 8 cut(s) 777, 1223, 1917, 2579, 3018, 3614, 3713, 3719
StuI AGGCCT 3 cut(s) 1760, 1928, 2399
StyD4I CCNGG 8 cut(s) 48, 176, 477, 1332, 2334, 2500, 3405, 3738
StyI CCWWGG 7 cut(s) 231, 561, 1018, 1776, 1983, 2400, 2514
TaaI ACNGT 6 cut(s) 10, 1424, 1623, 1880, 1951, 2648
TaiI ACGT 6 cut(s) 66, 279, 853, 2986, 3139, 3386
TatI WGTACW 1 cut(s) 2931
TauI GCSGC 2 cut(s) 219, 266
TscAI CASTG 6 cut(s) 376, 792, 907, 2653, 2855, 3444
TseFI GTSAC 4 cut(s) 1424, 1598, 1825, 3895
TseI GCWGC 7 cut(s) 659, 741, 1327, 2153, 3499, 3767, 3770
Tsp45I GTSAC 4 cut(s) 1424, 1598, 1825, 3895
TspGWI ACGGA 1 cut(s) 3759
TspRI CASTG 6 cut(s) 376, 792, 907, 2653, 2855, 3444
Vha464I CTTAAG 1 cut(s) 2906
VpaK11BI GGWCC 5 cut(s) 180, 1282, 3403, 3746, 3793
VspI ATTAAT 1 cut(s) 360
XapI RAATTY 7 cut(s) 765, 945, 966, 1120, 2126, 3030, 3200
XbaI TCTAGA 1 cut(s) 1222
XceI RCATGY 4 cut(s) 1462, 2242, 2573, 3878
XhoI CTCGAG 1 cut(s) 1575
XmiI GTMKAC 3 cut(s) 557, 3139, 3538
XmnI GAANNNNTTC 1 cut(s) 2762
XspI CTAG 8 cut(s) 777, 1223, 1917, 2579, 3018, 3614, 3713, 3719
ZraI GACGTC 2 cut(s) 277, 3384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.