Rroxscaffold_5G00357470

DNA repair protein RAD50

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0012062
Physical Location & Seq
Reverse (-)
37091243 .. 37105341
14099 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00357470.1

Sequence Viewer

Length: 3765 bp
ATGAGTACGGTGGACAAGATGCTTATAAAGGGAATCCGGAGCTTCGACCCGGAGAACAAGCACGTCATCACCTTCTTCAAACCGCTAACCCTAATCGTCGGCCACAACGGCGCCGGAAAAACCACCATCATCGAGTGCCTCAAGCTCTCTTGCACCGGCGAGTTGCCTCCCAATGCCAGGTCCGGCCACAGCTTCATCCACGACCCCAAAGTCGCCGGCGAAACCGAGACCAAAGGTCAGATTAAGCTCCGCTTCAAGACCGCCGCCGGCAAGGACGTCGTCTCTATCAGGTCGTTTCAGCTCACACAGAAAGCCTCCAAAATGGAGTATAAGGCCATCGAGAGCGTCCTTCAAACTATTAATCCCCACACCGGAGAGAAAGTTTGCCTGAGCTATAGATGTGCTGACATGGATAAGGAAATCCCTGCTTTGATGGGTGTCTCGAAGGCGATTCTCGAAAATGTGATCTTTGCTCACCAAGACGAAGCCAATTGGCCTTTGCAGGATCCTTCCACACTGAAGAAGAAGTTTGATGATATCTTCTCTGCTACACGGTATACCAAGGCATTGGAGGTAATTAAAAAACTTCACAAGGATCAAGCTCAAGAGATAAAGACCTACAAGCTAAAATTGGAGAATCTTCAAACCTTGAAAGATGCTGCATATAAGTTTCGGGAAAGCATTGCTAAAGATGAGGAAAGAACAGAATTAGTGAAAAGACAGATGCAGGAGTTGGAGGGCAGTGTCCATGATCTGGATTCTAAAATTCATAATGCTAGAGAAACACTGAAGCATCTGCGGAACCTGGATGGCCAGCTATCTGGAAAGACTGCTGAAAGAAAAACTCTAGTTAAGGAGGTGGAGAGACAGTATAGAGATCTTGCAGAAGAAAATGAAGACACTGATGAACAGTTGATGGAATGGAAAACTACGTTTGAAGACAGAATTCGAGAGTTAGGAACTAAAATTCGTAGATTGGAGAGGGAACAGGATGACACAAAGACCAAAAGTAATGTCCTTACGCAGACAATAAAGGATTCTATACGGCAGATTAGCAGGCTTGAAAATGAAGCTGAGGCTCATTTGGAATTAAAGAAAAATTCAAATGAAACTGAAGTCAAGAAAGCATGGGACCTCTACATGGATGCAAATGATCGTTGGAAATTAAAAGAGGCTCAGCACCAGGCTAAAGCAGAAATAAAGAATGGCCTTTTGAAGCGTATTGAGGAGAAGAAACAGGAGCGTGATTCTTTTGAAAGAGATGTTTTAAACTGTAACCTTTCTCGCATTGATGAGAAAGAAAAAAACATGCGAATTGAGGTTGACAGAAAGGCAAATCAGCTTGCTGTTAGAGAATTTGATTCAACCATTCGTCAAAAGCAGAGTGAGGTGTTTAGCATTGACCAAATGATCACTGCAGTTACTCGAGAGAAGAACGTCTTGGATGGTGATAGGGATGACAGAATAATACTGTCTCACAAGAAGACAGACTTGGAGACTCAGAAAAAGAAACACAGAAAGATAATTGATGACTACAAGGACAGAATAAGAGGAGTGCTAAAAGGAAGGCTTCCTCCTGACAAGGATTTGAAAAGTGAAATCACTCAGGCCTTGAGGGTTGTTACCATGGAGTTTGATGACTTGAGCGCCAAATCTCGTGAAGTGGAGAAAGAGGTGAATATGTTTCAGATGAAAATACAAGAAGTTAATAATAACCTCTGTAAACACCGTAAAGATCTGGAATCAAAGAGGAGATATATTGAAACTAGACTTCAGGCCTTAGATCAGCAATGTTTTACTGTTGATTCCTACACCAAAGTCTTGGATTCAGCGAAGGAGAAAAGAGATGTGCATAAAAGCAAATACAATTTTGCAGATGGTATGCGACAGATGTTTGATCCTTTTGAAAGAGTTGCTCGTGCCCATCATATTTGTCCTTGTTGTGAACGCCCTTTCTCTCCAGAAGAGGAAGACGAATTCGTTAAAAAAGTGAAGGCAGCAACTTCTTCTGAGCAAATAAAAGCACTCTTAGTGGAATCCTCAAGTGCTGAGTCTTTTTTTCAGCAAGTGGACAAGCTTCGCTTGTTTTATGAAGAATATATAAAAATAGGCAAGGAAGCAATCCCTAATGCAGAGAAAGAACTATCTGAGCTTAGTGAAGAGATGGAACAAAAATCCCAAGCCCTTGATGATGTGTTGGCTGTTTCTGCTCAAGTAAAGTCTGATAAGGACTTGATTGAGGCCTTGGTGCAACCCATTGAAACTGCTGATAGACTACTTCAAGAAATACAAATACGCCAGAAGGACGTTGATGACTTGGAGTATAAGCTTGATATTCAAGGGCCAGGTGCAAGATCCTTGGAGGATATTCAGGACAATTTGCATGGTGAACTAGAGAAATTAAGAGAGGAACAAAGGTACATGGAAGGTGATTTATCAAACATTCAAATGCGGTGGCACAGTGTAAGGGAAGAGAAAATCAATGCCACTACTATGTTTCGTGATTTTGAAAGGACAGAAGAAGAGTTAGAGCGTTTGACCGATGAAAAGAGTCAAATTGATCTTGATGAAAAGCTTCAGGCAGAGGCTCTTGGCCCTTTATCAAGGGAGAAAGATAAATTACTTAATGACTACAATGAAATTAAAGCTGAACTCGGTCGCCAGTTCGAGGAGCAAGCTGAACAAAGCAGAAATTACAAGCACAAAGTTGATGAACTACTTAAGATTATTTCTGACATTAAAGAGTATAATGATTTAAGGAAAGGAGAGAGGTTGCAGGAACTGCAAGAGAAGCAACGTCTATCAGAATCTCAACTTCAAAGTTGTGATACTAGAATGCAGGAAATTTCAGATGAATTGGGCAGAAGTAATAAGTTAATGGAGGGTCAAGAGGAACTAAGGCGAAATATAGATGCCAATTTGAATTACAGGAAAACCATAGCTGAAGTAGATCAACTTACTCAAGAGGTCGTATCACTTGAAGCAGAAATCCTTAAATTCGGTGGGATTCCCAAGTGTGAAGCTGAACTCGTAAAGCTCTCACAGGAGAGAGAGAGGCTTCTTTCAGAGTTAAACAAGTTTCATGGAACAATATCGGTTTACCAGAGCAATATTTCAAATTATAAAATTGATCTCAAGCAGGCACAATACAAGGACATTGACAAACGGTACTTTGATCAGCTTATCCAGCTAAAGACATCTGAGATGGCAAACAAGGACCTGGACAGATATTACAATGCGCTTGACAAAGCACTGATGCGCTTCCACTCGATGAAGATGGAGGAAATAAATAAGATTATACGGGAGTTGTGGCAGCAGACTTACAGAGGACAAGATATAGATTATATCTGCATACATTCAGATTCTGAAAGTGCTGGGACTCGTTCATATAGCTACAAGGTTCTCATGCAGACTGGCGATACAGAACTAGAAATGAGAGGAAGATGTAGTGCTGGTCAGAAGGTCCTTGCTTCCCTGATCATAAGGTTGGCTTTAGCTGAAACTTTTTGCCTCAATTGTGGAATACTAGCACTAGATGAACCAACTACAAACTTGGACGGACCAAATGCTGAGAGTCTTGCTGCTGCTCTTGTTAGAATTATGGAGGACCGGAAAGGGCAGGAGAACTTTCAGTTGATAGTAATTACTCATGATGAGCGCTTTGCTCAACTAATTGGCCAGCGCCAGCATGCAGAAAAATATTATCGGGTGACAAAGGACGATCACCAGCATAGCATAATTGAAGCCCAGGAGATTTTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000228 GO:0000280 GO:0000722 GO:0000723 GO:0000724 GO:0000725 GO:0000781 GO:0000784 GO:0000785 GO:0000790 GO:0000793 GO:0000794 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003691 GO:0003697 GO:0003824 GO:0004017 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006278 GO:0006281 GO:0006302 GO:0006310 GO:0006312 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007004 GO:0007049 GO:0007127 GO:0007131 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009117 GO:0009123 GO:0009165 GO:0009987 GO:0010833 GO:0016043 GO:0016233 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016776 GO:0018130 GO:0019205 GO:0019438 GO:0019637 GO:0022402 GO:0022414 GO:0030870 GO:0031974 GO:0031981 GO:0032200 GO:0032392 GO:0032508 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0035825 GO:0035861 GO:0042162 GO:0042592 GO:0043047 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0046940 GO:0048285 GO:0050145 GO:0050896 GO:0051276 GO:0051321 GO:0051716 GO:0051880 GO:0055086 GO:0060249 GO:0061982 GO:0065007 GO:0065008 GO:0070013 GO:0070192 GO:0071103 GO:0071704 GO:0071840 GO:0071897 GO:0090304 GO:0090305 GO:0090407 GO:0090734 GO:0097159 GO:0098687 GO:0098847 GO:0140013 GO:1901293 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1903046
KEGG Pathways
Metabolic & Signaling
ko03440 ko03450 ko04218 map03440 map03450 map04218
Pfam Domains
Protein Families
AAA_23 Rad50_zn_hook

Protein Analysis

1254

Amino Acids

146.01

Weight (kDa)

5.73

Isoelectric Point (pI)

41.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SMC_N PF02463 4 - 105 4.5e-06 RecF/RecN/SMC N terminal domain
AAA_23 PF13476 6 - 215 3e-33 AAA domain
SMC_N PF02463 1014 - 1240 4.6e-09 RecF/RecN/SMC N terminal domain
SbcC_Walker_B PF13558 1128 - 1197 2.7e-06 SbcC/RAD50-like, Walker B motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012062)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G31970
fragaria_vesca FvH4_4g13300
malus_domestica MD05G1230300.v1.1
prunus_persica Prupe.1G165000_v2.0.a1 Prupe.1G165000_v2.0.a1 Prupe.1G165000_v2.0.a1 Prupe.1G165000_v2.0.a1 Prupe.1G165000_v2.0.a1
pyrus_communis pycom04g01350
rosa_chinensis RchiOBHm_Chr4g0413591
rosa_laevigata RLG00000008210
rosa_multiflora Rmu_sc0014097.1_g000002
rosa_roxburghii Rroxscaffold_5G00357470
rosa_rugosa Rorug04G0121100
rosa_samantha Rh4AG180900 Rh4BG180100 Rh4CG192000 Rh4DG176200
rosa_wichuraiana Rw4G015110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 26, 3123
AasI GACNNNNNNGTC 1 cut(s) 209
AatII GACGTC 1 cut(s) 279
AccB1I GGYRCC 1 cut(s) 110
AccB7I CCANNNNNTGG 1 cut(s) 754
AccI GTMKAC 1 cut(s) 557
AccIII TCCGGA 1 cut(s) 36
AciI CCGC 6 cut(s) 83, 250, 261, 264, 799, 2452
AclWI GGATC 5 cut(s) 500, 513, 603, 1892, 2349
AcoI YGGCCR 4 cut(s) 100, 184, 811, 3676
AcsI RAATTY 8 cut(s) 765, 945, 966, 1099, 1355, 1976, 2844, 2996
AcuI CTGAAG 6 cut(s) 539, 809, 1134, 1757, 2561, 2964
AcyI GRCGYC 2 cut(s) 111, 276
AfaI GTAC 3 cut(s) 7, 2420, 3170
AfeI AGCGCT 1 cut(s) 3659
AfiI CCNNNNNNNGG 5 cut(s) 177, 371, 754, 1141, 2668
AflII CTTAAG 1 cut(s) 2720
AhdI GACNNNNNGTC 1 cut(s) 234
AjiI CACGTC 1 cut(s) 64
AjnI CCWGG 6 cut(s) 176, 804, 1182, 2344, 3219, 3747
AjuI GAANNNNNNNTTGG 4 cut(s) 1424, 1456, 1475, 1507
Alw26I GTCTC 6 cut(s) 221, 286, 445, 859, 1479, 1490
AlwI GGATC 5 cut(s) 500, 513, 603, 1892, 2349
AlwNI CAGNNNCTG 2 cut(s) 2782, 3365
Ama87I CYCGRG 1 cut(s) 1425
Aor13HI TCCGGA 1 cut(s) 36
Aor51HI AGCGCT 1 cut(s) 3659
ApeKI GCWGC 5 cut(s) 659, 1997, 3313, 3581, 3584
ApoI RAATTY 8 cut(s) 765, 945, 966, 1099, 1355, 1976, 2844, 2996
ArsI GACNNNNNNTTYG 2 cut(s) 999, 1031
AseI ATTAAT 1 cut(s) 360
Asp700I GAANNNNTTC 1 cut(s) 2574
AspLEI GCGC 6 cut(s) 113, 1649, 3241, 3261, 3660, 3684
AspS9I GGNCC 8 cut(s) 180, 1132, 2342, 2594, 3217, 3463, 3560, 3607
AsuC2I CCSGG 1 cut(s) 50
AsuHPI GGTGA 8 cut(s) 61, 467, 1460, 1687, 2399, 2441, 3716, 3721
AvaI CYCGRG 1 cut(s) 1425
AvaII GGWCC 6 cut(s) 180, 1132, 3217, 3463, 3560, 3607
BaeGI GKGCMC 1 cut(s) 1924
BalI TGGCCA 2 cut(s) 813, 3678
BamHI GGATCC 1 cut(s) 505
BanI GGYRCC 1 cut(s) 110
BauI CACGAG 2 cut(s) 1656, 1917
BbsI GAAGAC 4 cut(s) 903, 945, 1490, 1977
BbvCI CCTCAGC 1 cut(s) 1074
BbvI GCAGC 5 cut(s) 646, 2009, 3325, 3568, 3571
BceAI ACGGC 2 cut(s) 124, 1061
BcgI CGANNNNNNTGC 2 cut(s) 259, 293
BciT130I CCWGG 6 cut(s) 178, 806, 1184, 2346, 3221, 3749
BclI TGATCA 3 cut(s) 1410, 3175, 3477
BcnI CCSGG 1 cut(s) 50
BcoDI GTCTC 6 cut(s) 221, 286, 445, 859, 1479, 1490
BfaI CTAG 8 cut(s) 777, 848, 1767, 2393, 2832, 3428, 3527, 3533
BfmI CTRYAG 2 cut(s) 394, 1416
BfoI RGCGCY 4 cut(s) 114, 1650, 3661, 3685
BfrI CTTAAG 1 cut(s) 2720
BglI GCCNNNNNGGC 1 cut(s) 108
BglII AGATCT 2 cut(s) 877, 1735
BisI GCNGC 6 cut(s) 264, 660, 1998, 3314, 3582, 3585
BlpI GCTNAGC 1 cut(s) 1176
BlsI GCNGC 6 cut(s) 265, 661, 1999, 3315, 3583, 3586
Bme1390I CCNGG 7 cut(s) 50, 178, 806, 1184, 2346, 3221, 3749
Bme18I GGWCC 6 cut(s) 180, 1132, 3217, 3463, 3560, 3607
BmeRI GACNNNNNGTC 1 cut(s) 234
BmeT110I CYCGRG 1 cut(s) 1425
BmgBI CACGTC 1 cut(s) 64
BmgT120I GGNCC 8 cut(s) 180, 1132, 2342, 2594, 3217, 3463, 3560, 3607
BmiI GGNNCC 4 cut(s) 112, 507, 803, 1133
BmrFI CCNGG 7 cut(s) 50, 178, 806, 1184, 2346, 3221, 3749
BmsI GCATC 7 cut(s) 9, 646, 714, 802, 1135, 2902, 3246
BpiI GAAGAC 4 cut(s) 903, 945, 1490, 1977
BpmI CTGGAG 1 cut(s) 1944
Bpu10I CCTNAGC 2 cut(s) 389, 1074
Bpu1102I GCTNAGC 1 cut(s) 1176
BpuEI CTTGAG 8 cut(s) 125, 588, 1633, 1663, 2026, 2196, 2946, 3119
BpuMI CCSGG 1 cut(s) 50
BsaHI GRCGYC 2 cut(s) 111, 276
BsaI GGTCTC 1 cut(s) 221
BsaJI CCNNGG 5 cut(s) 561, 1626, 2244, 2358, 3747
BsaWI WCCGGW 3 cut(s) 36, 371, 3609
BsaXI ACNNNNNCTCC 4 cut(s) 366, 396, 854, 884
Bsc4I CCNNNNNNNGG 5 cut(s) 177, 371, 754, 1141, 2668
Bse118I RCCGGY 3 cut(s) 155, 215, 266
Bse1I ACTGG 2 cut(s) 2662, 3418
Bse3DI GCAATG 2 cut(s) 681, 1796
BseAI TCCGGA 1 cut(s) 36
BseBI CCWGG 6 cut(s) 178, 806, 1184, 2346, 3221, 3749
BseDI CCNNGG 5 cut(s) 561, 1626, 2244, 2358, 3747
BseGI GGATG 6 cut(s) 195, 814, 997, 1150, 1450, 1462
BseLI CCNNNNNNNGG 5 cut(s) 177, 371, 754, 1141, 2668
BseMI GCAATG 2 cut(s) 681, 1796
BseNI ACTGG 2 cut(s) 2662, 3418
BseRI GAGGAG 4 cut(s) 1241, 1566, 1765, 2684
BseSI GKGCMC 1 cut(s) 1924
BseXI GCAGC 5 cut(s) 646, 2009, 3325, 3568, 3571
BseYI CCCAGC 1 cut(s) 3374
Bsh1285I CGRYCG 1 cut(s) 2659
BshNI GGYRCC 1 cut(s) 110
BsiEI CGRYCG 1 cut(s) 2659
BsiHKCI CYCGRG 1 cut(s) 1425
BsiSI CCGG 9 cut(s) 37, 50, 114, 156, 183, 216, 267, 372, 3610
BslFI GGGAC 2 cut(s) 1145, 3391
BslI CCNNNNNNNGG 5 cut(s) 177, 371, 754, 1141, 2668
BsmAI GTCTC 6 cut(s) 221, 286, 445, 859, 1479, 1490
BsmBI CGTCTC 1 cut(s) 286
BsmFI GGGAC 2 cut(s) 1145, 3391
BsmI GAATGC 1 cut(s) 2841
Bso31I GGTCTC 1 cut(s) 221
BsoBI CYCGRG 1 cut(s) 1425
Bsp1286I GDGCHC 1 cut(s) 1924
Bsp13I TCCGGA 1 cut(s) 36
Bsp1720I GCTNAGC 1 cut(s) 1176
Bsp19I CCATGG 1 cut(s) 1626
BspACI CCGC 6 cut(s) 83, 250, 261, 264, 799, 2452
BspEI TCCGGA 1 cut(s) 36
BspHI TCATGA 1 cut(s) 3649
BspLI GGNNCC 4 cut(s) 112, 507, 803, 1133
BspMAI CTGCAG 1 cut(s) 1420
BspPI GGATC 5 cut(s) 500, 513, 603, 1892, 2349
BspT107I GGYRCC 1 cut(s) 110
BspTI CTTAAG 1 cut(s) 2720
BspTNI GGTCTC 1 cut(s) 221
BsrDI GCAATG 2 cut(s) 681, 1796
BsrFI RCCGGY 3 cut(s) 155, 215, 266
BsrI ACTGG 2 cut(s) 2662, 3418
BssAI RCCGGY 3 cut(s) 155, 215, 266
BssECI CCNNGG 5 cut(s) 561, 1626, 2244, 2358, 3747
BssNAI GTATAC 1 cut(s) 558
BssNI GRCGYC 2 cut(s) 111, 276
BssSI CACGAG 2 cut(s) 1656, 1917
BssT1I CCWWGG 4 cut(s) 561, 1626, 2244, 2358
Bst1107I GTATAC 1 cut(s) 558
Bst2BI CACGAG 2 cut(s) 1656, 1917
Bst2UI CCWGG 6 cut(s) 178, 806, 1184, 2346, 3221, 3749
Bst6I CTCTTC 4 cut(s) 1959, 2154, 2466, 2517
BstACI GRCGYC 2 cut(s) 111, 276
BstAFI CTTAAG 1 cut(s) 2720
BstAPI GCANNNNNTGC 1 cut(s) 2782
BstDSI CCRYGG 1 cut(s) 1626
BstF5I GGATG 6 cut(s) 195, 814, 997, 1150, 1450, 1462
BstH2I RGCGCY 4 cut(s) 114, 1650, 3661, 3685
BstHHI GCGC 6 cut(s) 113, 1649, 3241, 3261, 3660, 3684
BstMAI GTCTC 6 cut(s) 221, 286, 445, 859, 1479, 1490
BstMCI CGRYCG 1 cut(s) 2659
BstMWI GCNNNNNNNGC 5 cut(s) 108, 2207, 2782, 2791, 3187
BstNI CCWGG 6 cut(s) 178, 806, 1184, 2346, 3221, 3749
BstNSI RCATGY 2 cut(s) 1312, 3692
BstSCI CCNGG 7 cut(s) 48, 176, 804, 1182, 2344, 3219, 3747
BstSFI CTRYAG 2 cut(s) 394, 1416
BstSLI GKGCMC 1 cut(s) 1924
BstV1I GCAGC 5 cut(s) 646, 2009, 3325, 3568, 3571
BstV2I GAAGAC 4 cut(s) 903, 945, 1490, 1977
BstX2I RGATCY 4 cut(s) 505, 877, 1735, 2354
BstXI CCANNNNNNTGG 3 cut(s) 568, 821, 1822
BstYI RGATCY 4 cut(s) 505, 877, 1735, 2354
BstZ17I GTATAC 1 cut(s) 558
BtgI CCRYGG 1 cut(s) 1626
BtrI CACGTC 1 cut(s) 64
BtsCI GGATG 6 cut(s) 195, 814, 997, 1150, 1450, 1462
BtsI GCAGTG 2 cut(s) 748, 1413
BtsIMutI CAGTG 7 cut(s) 515, 748, 785, 900, 1413, 2467, 3251
CaiI CAGNNNCTG 2 cut(s) 2782, 3365
CciI TCATGA 1 cut(s) 3649
CfoI GCGC 6 cut(s) 113, 1649, 3241, 3261, 3660, 3684
Cfr10I RCCGGY 3 cut(s) 155, 215, 266
Cfr13I GGNCC 8 cut(s) 180, 1132, 2342, 2594, 3217, 3463, 3560, 3607
CseI GACGC 1 cut(s) 334
Csp6I GTAC 3 cut(s) 6, 2419, 3169
CspCI CAANNNNNGTGG 2 cut(s) 2435, 2470
CviQI GTAC 3 cut(s) 6, 2419, 3169
DinI GGCGCC 1 cut(s) 112
DraI TTTAAA 1 cut(s) 1269
DrdI GACNNNNNNGTC 1 cut(s) 209
DriI GACNNNNNGTC 1 cut(s) 234
DseDI GACNNNNNNGTC 1 cut(s) 209
EaeI YGGCCR 4 cut(s) 100, 184, 811, 3676
Eam1104I CTCTTC 4 cut(s) 1959, 2154, 2466, 2517
Eam1105I GACNNNNNGTC 1 cut(s) 234
EarI CTCTTC 4 cut(s) 1959, 2154, 2466, 2517
Eco130I CCWWGG 4 cut(s) 561, 1626, 2244, 2358
Eco147I AGGCCT 3 cut(s) 1610, 1778, 2243
Eco31I GGTCTC 1 cut(s) 221
Eco32I GATATC 1 cut(s) 538
Eco47I GGWCC 6 cut(s) 180, 1132, 3217, 3463, 3560, 3607
Eco47III AGCGCT 1 cut(s) 3659
Eco57I CTGAAG 6 cut(s) 539, 809, 1134, 1757, 2561, 2964
Eco88I CYCGRG 1 cut(s) 1425
EcoO109I RGGNCCY 3 cut(s) 1132, 3217, 3463
EcoRI GAATTC 2 cut(s) 945, 1976
EcoRII CCWGG 6 cut(s) 176, 804, 1182, 2344, 3219, 3747
EcoRV GATATC 1 cut(s) 538
EcoT14I CCWWGG 4 cut(s) 561, 1626, 2244, 2358
EgeI GGCGCC 1 cut(s) 112
EheI GGCGCC 1 cut(s) 112
ErhI CCWWGG 4 cut(s) 561, 1626, 2244, 2358
Esp3I CGTCTC 1 cut(s) 286
FaqI GGGAC 2 cut(s) 1145, 3391
FbaI TGATCA 3 cut(s) 1410, 3175, 3477
FblI GTMKAC 1 cut(s) 557
Fnu4HI GCNGC 6 cut(s) 264, 660, 1998, 3314, 3582, 3585
FokI GGATG 6 cut(s) 182, 821, 1004, 1157, 1457, 1469
Fsp4HI GCNGC 6 cut(s) 264, 660, 1998, 3314, 3582, 3585
FspBI CTAG 8 cut(s) 777, 848, 1767, 2393, 2832, 3428, 3527, 3533
GlaI GCGC 6 cut(s) 112, 1648, 3240, 3260, 3659, 3683
GluI GCNGC 6 cut(s) 264, 660, 1998, 3314, 3582, 3585
GsaI CCCAGC 1 cut(s) 3378
GsuI CTGGAG 1 cut(s) 1944
HaeII RGCGCY 4 cut(s) 114, 1650, 3661, 3685
HapII CCGG 9 cut(s) 37, 50, 114, 156, 183, 216, 267, 372, 3610
HgaI GACGC 1 cut(s) 334
HhaI GCGC 6 cut(s) 113, 1649, 3241, 3261, 3660, 3684
Hin1I GRCGYC 2 cut(s) 111, 276
Hin6I GCGC 6 cut(s) 111, 1647, 3239, 3259, 3658, 3682
HinP1I GCGC 6 cut(s) 111, 1647, 3239, 3259, 3658, 3682
HincII GTYRAC 1 cut(s) 1324
HindII GTYRAC 1 cut(s) 1324
HindIII AAGCTT 3 cut(s) 2075, 2327, 2573
HpaII CCGG 9 cut(s) 37, 50, 114, 156, 183, 216, 267, 372, 3610
HphI GGTGA 8 cut(s) 61, 467, 1460, 1687, 2399, 2441, 3716, 3721
Hpy166II GTNNAC 8 cut(s) 13, 558, 1324, 1724, 1946, 2071, 2390, 3100
Hpy8I GTNNAC 8 cut(s) 13, 558, 1324, 1724, 1946, 2071, 2390, 3100
Hpy99I CGWCG 2 cut(s) 101, 281
HpyCH4IV ACGT 6 cut(s) 63, 276, 932, 1437, 2307, 2797
HpyF10VI GCNNNNNNNGC 5 cut(s) 108, 2207, 2782, 2791, 3187
HpySE526I ACGT 6 cut(s) 63, 276, 932, 1437, 2307, 2797
Hsp92I GRCGYC 2 cut(s) 111, 276
HspAI GCGC 6 cut(s) 111, 1647, 3239, 3259, 3658, 3682
KasI GGCGCC 1 cut(s) 110
Kpn2I TCCGGA 1 cut(s) 36
KroI GCCGGC 2 cut(s) 215, 266
KroNI GCCGGC 2 cut(s) 217, 268
Ksp22I TGATCA 3 cut(s) 1410, 3175, 3477
LmnI GCTCC 4 cut(s) 39, 252, 1240, 2671
Lsp1109I GCAGC 5 cut(s) 646, 2009, 3325, 3568, 3571
LweI GCATC 7 cut(s) 9, 646, 714, 802, 1135, 2902, 3246
MaeI CTAG 8 cut(s) 777, 848, 1767, 2393, 2832, 3428, 3527, 3533
MaeII ACGT 6 cut(s) 63, 276, 932, 1437, 2307, 2797
MaeIII GTNAC 4 cut(s) 1274, 1420, 1621, 3709
MfeI CAATTG 2 cut(s) 490, 3514
MflI RGATCY 4 cut(s) 505, 877, 1735, 2354
MhlI GDGCHC 1 cut(s) 1924
MlsI TGGCCA 2 cut(s) 813, 3678
MluNI TGGCCA 2 cut(s) 813, 3678
Mly113I GGCGCC 1 cut(s) 111
MlyI GAGTC 5 cut(s) 1492, 2060, 2560, 3373, 3583
MmeI TCCRAC 2 cut(s) 714, 1139
Mox20I TGGCCA 2 cut(s) 813, 3678
MreI CGCCGGCG 1 cut(s) 215
MroI TCCGGA 1 cut(s) 36
MroNI GCCGGC 2 cut(s) 215, 266
MroXI GAANNNNTTC 1 cut(s) 2574
MscI TGGCCA 2 cut(s) 813, 3678
MslI CAYNNNNRTG 2 cut(s) 2447, 2492
Msp20I TGGCCA 2 cut(s) 813, 3678
MspCI CTTAAG 1 cut(s) 2720
MspI CCGG 9 cut(s) 37, 50, 114, 156, 183, 216, 267, 372, 3610
MspR9I CCNGG 7 cut(s) 50, 178, 806, 1184, 2346, 3221, 3749
MunI CAATTG 2 cut(s) 490, 3514
Mva1269I GAATGC 1 cut(s) 2841
MvaI CCWGG 6 cut(s) 178, 806, 1184, 2346, 3221, 3749
MwoI GCNNNNNNNGC 5 cut(s) 108, 2207, 2782, 2791, 3187
NaeI GCCGGC 2 cut(s) 217, 268
NarI GGCGCC 1 cut(s) 111
NciI CCSGG 1 cut(s) 50
NcoI CCATGG 1 cut(s) 1626
NgoMIV GCCGGC 2 cut(s) 215, 266
NlaIV GGNNCC 4 cut(s) 112, 507, 803, 1133
NmuCI GTSAC 1 cut(s) 3709
NspI RCATGY 2 cut(s) 1312, 3692
PaeI GCATGC 1 cut(s) 3692
PaeR7I CTCGAG 1 cut(s) 1425
PagI TCATGA 1 cut(s) 3649
PceI AGGCCT 3 cut(s) 1610, 1778, 2243
PctI GAATGC 1 cut(s) 2841
PdiI GCCGGC 2 cut(s) 217, 268
PdmI GAANNNNTTC 1 cut(s) 2574
PflFI GACNNNGTC 1 cut(s) 278
PflMI CCANNNNNTGG 1 cut(s) 754
PkrI GCNGC 6 cut(s) 265, 661, 1999, 3315, 3583, 3586
PleI GAGTC 5 cut(s) 1492, 2059, 2559, 3373, 3582
PluTI GGCGCC 1 cut(s) 114
PpsI GAGTC 5 cut(s) 1492, 2059, 2559, 3373, 3582
PpuMI RGGWCCY 3 cut(s) 1132, 3217, 3463
PshBI ATTAAT 1 cut(s) 360
PsiI TTATAA 2 cut(s) 26, 3123
Psp5II RGGWCCY 3 cut(s) 1132, 3217, 3463
Psp6I CCWGG 6 cut(s) 176, 804, 1182, 2344, 3219, 3747
PspFI CCCAGC 1 cut(s) 3374
PspGI CCWGG 6 cut(s) 176, 804, 1182, 2344, 3219, 3747
PspN4I GGNNCC 4 cut(s) 112, 507, 803, 1133
PspPI GGNCC 8 cut(s) 180, 1132, 2342, 2594, 3217, 3463, 3560, 3607
PspPPI RGGWCCY 3 cut(s) 1132, 3217, 3463
PstI CTGCAG 1 cut(s) 1420
PstNI CAGNNNCTG 2 cut(s) 2782, 3365
PsuI RGATCY 4 cut(s) 505, 877, 1735, 2354
PsyI GACNNNGTC 1 cut(s) 278
RsaI GTAC 3 cut(s) 7, 2420, 3170
RsaNI GTAC 3 cut(s) 6, 2419, 3169
RseI CAYNNNNRTG 2 cut(s) 2447, 2492
SatI GCNGC 6 cut(s) 264, 660, 1998, 3314, 3582, 3585
Sau96I GGNCC 8 cut(s) 180, 1132, 2342, 2594, 3217, 3463, 3560, 3607
SchI GAGTC 5 cut(s) 1492, 2060, 2560, 3373, 3583
ScrFI CCNGG 7 cut(s) 50, 178, 806, 1184, 2346, 3221, 3749
SduI GDGCHC 1 cut(s) 1924
SfaNI GCATC 7 cut(s) 9, 646, 714, 802, 1135, 2902, 3246
SfcI CTRYAG 2 cut(s) 394, 1416
SfoI GGCGCC 1 cut(s) 112
Sfr274I CTCGAG 1 cut(s) 1425
SgrAI CRCCGGYG 2 cut(s) 155, 215
SinI GGWCC 6 cut(s) 180, 1132, 3217, 3463, 3560, 3607
SlaI CTCGAG 1 cut(s) 1425
SmiMI CAYNNNNRTG 2 cut(s) 2447, 2492
SphI GCATGC 1 cut(s) 3692
SseBI AGGCCT 3 cut(s) 1610, 1778, 2243
SsiI CCGC 6 cut(s) 83, 250, 261, 264, 799, 2452
SspDI GGCGCC 1 cut(s) 110
SspI AATATT 2 cut(s) 3112, 3701
SspMI CTAG 8 cut(s) 777, 848, 1767, 2393, 2832, 3428, 3527, 3533
StuI AGGCCT 3 cut(s) 1610, 1778, 2243
StyD4I CCNGG 7 cut(s) 48, 176, 804, 1182, 2344, 3219, 3747
StyI CCWWGG 4 cut(s) 561, 1626, 2244, 2358
TaiI ACGT 6 cut(s) 66, 279, 935, 1440, 2310, 2800
TaqI TCGA 9 cut(s) 45, 132, 339, 443, 456, 949, 1426, 2667, 3269
TaqII GACCGA 2 cut(s) 2555, 2645
TauI GCSGC 1 cut(s) 266
TscAI CASTG 7 cut(s) 522, 748, 792, 907, 1420, 2467, 3258
TseFI GTSAC 1 cut(s) 3709
TseI GCWGC 5 cut(s) 659, 1997, 3313, 3581, 3584
Tsp45I GTSAC 1 cut(s) 3709
TspGWI ACGGA 1 cut(s) 3573
TspRI CASTG 7 cut(s) 522, 748, 792, 907, 1420, 2467, 3258
Tth111I GACNNNGTC 1 cut(s) 278
Van91I CCANNNNNTGG 1 cut(s) 754
Vha464I CTTAAG 1 cut(s) 2720
VpaK11BI GGWCC 6 cut(s) 180, 1132, 3217, 3463, 3560, 3607
VspI ATTAAT 1 cut(s) 360
XapI RAATTY 8 cut(s) 765, 945, 966, 1099, 1355, 1976, 2844, 2996
XceI RCATGY 2 cut(s) 1312, 3692
XhoI CTCGAG 1 cut(s) 1425
XmiI GTMKAC 1 cut(s) 557
XmnI GAANNNNTTC 1 cut(s) 2574
XspI CTAG 8 cut(s) 777, 848, 1767, 2393, 2832, 3428, 3527, 3533
ZraI GACGTC 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.