RLG00000008210

DNA repair protein RAD50

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0012062
Physical Location & Seq
Forward (+)
27145452 .. 27162384
16933 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008210

Sequence Viewer

Length: 3951 bp
ATGAGTACGGTGGACAAGATGCTTATAAAGGGAATCCGGAGCTTTGACCCGGAGAACAAGCACGTCATCACCTTCTTCAAGCCCTTAACCCTAATCGTCGGCCACAACGGCGCCGGCAAAACCACCATCATCGAGTGCCTCAAGCTCTCTTGCACCGGCGAGTTGCCTCCCAATGCCAGGTCCGGCCACAGCTTCATCCACGACCCCAAAGTCGCCGGCGAAACCGAGACCAAAGGTCAGATTAAGCTCCGCTTCAAGACGGCCGCCGGCAAGGACGTCGTCTCTATCAGGTCGTTTCAGCTCACTCAGAAAGCCTCCAAAATGGAGTATAAGGCCATCGAGAGCGTCCTTCAAACTATTAATCCCCACAGCGGAGAGAAAGTTTGCCTTAGCTATAGATGTGCTGACATGGATAAGGAAATCCCTGCTTTAATGGGTGTCTCAAAGGCAATTCTCGAGAATGTGATCTTTGCTCACCAAGACGAAGCGAATTGGCCTTTGCAAGATCCTTCCACACTGAAGAAAAAGTTTGATGATATCTTCTCTGCTACACGGTATACCAAGGCATTGGAGGTCATTAAAAAACTTCACAAGGATCAAGCTCAAGAGATAAAGACCTACAAGCTAAAATTGGAGAATCTTCAAACCTTGAAAGATGCTGCATATAAGTTTCGTGAAAGCATTGCTAAAGATGAGGAAAGAACAGAATTAGTGAAAAGACAGATGCAGGAGTTGGAGGGCAGTGTCCATGATCTGGATTCTAAAATTCATAATGCTAGAGAAACACTGAAGCATCTGCGGAACCTGGATGGCCAGCTATCTGCAAAGACTGCTGAAAGAAAAACTCTAGTCAAGGAGGTGGAGAGACAGTATAGAGATCTTGCAGAAGAAAATGAAGACACTGATGAAGAGTTGATGGAATGGAAAACTACGTTTGAAGACAGAATTCGAGAGTTAGGAACTAAAATTCGTAGATTGGAGAGGGAACAGGATGACACAAAGACCAAAAGTAATGTCCTTACGCAGACAATAAAGGATTCTATACGGCAGATTAGCAGGCTTGAACATGAAGCTGAGGCTCATTTGGAATTAAAGAAAGTGAGAGATTCAACCATTCAAAGTTTTTTTGCAAGGCACAATTTAGGTTCTCTTCCAAATCCCCCCTTCAGCAATGAAGTTGCTATAAACTTGACAAATAGAATAAAATCAAGGTTGTGTGATCTAGAAAAGGATTTGCAAGATAAGAAGAATTCAAATGAAACTGAAGTCAAGAAAGCATGGGACCTCTACATGGATGCAAATGATCGTTGGAAATTAAAAGAGGCTCAGCACCAGGCTAAAGCAGAAATAAAGAATGGCCTTTTGAAGCGTATTGAGGAGAAGAAACAGGAGCGTGATTCTTTTGAAAGAGATGTTTTAAACTGTAACCTTTCTCGCATTGATGAGAAAGAAAAAAACATGCGAATTGAGGTTGACAGAAAGGCAAATCAGCTTGCTGTTAGAGAATTTGATTCAACCATTCGTCAAAAGCAGAGTGAGGTGTTTAGCATGGACCAAATGATCAGTGCAGTTACTCGAGAGAAGAACGTCTTGGATGGTGATAGGGATGACAGAATACTACTGTCTCACAAGAAGACAGACTTGGAGACTCAGAAAAAGAAACACAGAAAGATAATTGATGACTACAAGGACAGAATAAGAGGAGTGCTAAAAGGAAGGCTTCCTCCTGACAAGGATTTGAAAAGTGAAATCACTCAGGCCTTGAGGATTGTTACCATGGAGTTTGATGACTTGAGCGCCAAATCTCGTGAAGTGGAGAAAGAGGTGAATATGTTTCAGATGAAAATACAAGAAGTTAATAATAACCTCTGTAAACACCGTAAAGATCTGGAATCAAAGAGGAGATATATTGAAAGTAGACTTCAGGCCTTAGATCAGCAATGTTTTACTGTTGATTCCTACACCAAAGTCTTGGATTCAGCGAAGGAGAAGAGAGATGTGCATAAAAGCAAATACAACTTTGCAGATGGTATGCGACAGATGTTTGATCCTTTTGAAAGAGTTGCTCGTGCCCATCATATTTGTCCTTGTTGTGAACGCCCTTTCTCTCCAGAAGAGGAAGACGAGTTCGTTAAAAAGCAAAAAGTGAAGGCAGCAACTTCTTCTGAGCAAATAAAAGCACTCTTTGTGGAATCCTCAAGTGCTGACTCTTTTTTTCAGCAAGTGGACAAGCTTCGCTTGATTTATGAAGAATATATAAAAATAGGCAAGGAAGCAATCCCTAATGCAGAGAAAGAACTATCTGAGCTTAGTGAAGAGATGGAACAAAAATCCCAAGCCCTTGATGATGTGTTGGCTGTTTCTGCTCAAGTAAAGTCTGATAAGGACTTGATTGAGTACTTGGTTCAACCCATTGAAACTGCTGATAGACTACTTCAAGAAATACAAATACACCAGAAGGACGTTGATGACTTGGAGTATAAGCTTGATATTCAAGGGCCAGGTGCAAGATCCTTGGAGGATATTCAGTTAGAGCTGACTAATCTGCAAAGAAAAAAGGACAATTTGCATGGTGATCTAGAGAAATTAAGAGAGGAACAAAGGTACATGGAAGGTGATTTATCAAACATTCAAATGCGGTGGCACAGTGTAAGGGAAGAGAAAATCAAGGCCACTACTATGTTTCGTGATTTTGAAAGGGCAGAAGAAGAGTTAGAGCGTTTGGCCGATGAAAAGAGTCAAATTGATCTCGATGAAAAGTTTCTGGCAGAGGCTCTTGGCCCTTTATCAAGGGAGAAAGATAAATTACTTAATGACTACAATGAAATTAAAGCTGAACTCGGTCGCCAGTGTGAGGAGCAAGCTGAACAAAGCAGAAATTACAAGCACAAAGTTGATGAACTACTTAAGATTATTTCTGACATTAAAGAGTATAATGATTTAAGGAAAGGAGAGAGGTTACAGGAAATGCAAGAGAAGCAACGTCTATCAGAATCTCAACTTCAAAGTTGTGATACTAGAATGCAGGAAATTTCAGATGAATTAGGCAGAAGTAATAAGTTAATGGAGGGTCAAGAGGAACTAAGGCGAAATATAGATGCCAATTTGAATTACCGGAAAACCAAAGCTGAAGTAGATCAACTTACTCAAGAGGTCGTATCACTCGAAGCAGAAGTCCTTAAATTTGGTGAGATTCCCAAGTGTGAAGCTGAACTCGTAAAGCTCTCACAGGAGAGAGAGAGGCTTCTTTCAGAGTTAAACAAGTTTCGTGGAACAATATCGGTTTACCAGAGCAATATTTCAAATTATAAAATTGATCTCAAACAGGCACAATACAAGGACATTGACAAACGGTACTTTGATCAGCTTATCCAGCTAAAGACATCTGAGATGGCAAACAAGGACCTGGACAGATATTACAATGCGCTTGACAAAGCACTGATGCGCTTCCACTCGATGAAGATGGAGGAAATAAATAAGATTATACGGGAGCTGTGGCAGCAGACTTACAGAGGACAAGATATAGATTATATCTGCATACATTCAGATTCTGAAAGTGCTGGGACTCGTTCATATAGCTACAAGGTTCTCATGCAGACTGGTGATACAGAACTAGAAATGAGAGGAAGATGTAGTGCTGGTCAGAAGGTCCTTGCTTCCCTGATCATAAGGTTGGCTTTAGCTGAAACTTTTTGCCTCAATTGTGGAATACTAGCACTAGATGAACCAACTACAAACTTGGATGGACCAAATGCTGAGAGTCTTGCTGCTGCTCTTGTTAGAATTATGGAGGACCGGAAAGGGCAGGAGAACTTTCAGTTGATAGTAATTACTCATGATGAGCGCTTTGCTCAACTAATTGGCCAGCGCCAGCATGCAGAAAAATATTATCGGGTGACAAAGGATGATCACCAGCATAGCATAATTGAAGCCCAGGAGATTTTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000228 GO:0000280 GO:0000722 GO:0000723 GO:0000724 GO:0000725 GO:0000781 GO:0000784 GO:0000785 GO:0000790 GO:0000793 GO:0000794 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003691 GO:0003697 GO:0003824 GO:0004017 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006278 GO:0006281 GO:0006302 GO:0006310 GO:0006312 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007004 GO:0007049 GO:0007127 GO:0007131 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009117 GO:0009123 GO:0009165 GO:0009987 GO:0010833 GO:0016043 GO:0016233 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016776 GO:0018130 GO:0019205 GO:0019438 GO:0019637 GO:0022402 GO:0022414 GO:0030870 GO:0031974 GO:0031981 GO:0032200 GO:0032392 GO:0032508 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0035825 GO:0035861 GO:0042162 GO:0042592 GO:0043047 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0046940 GO:0048285 GO:0050145 GO:0050896 GO:0051276 GO:0051321 GO:0051716 GO:0051880 GO:0055086 GO:0060249 GO:0061982 GO:0065007 GO:0065008 GO:0070013 GO:0070192 GO:0071103 GO:0071704 GO:0071840 GO:0071897 GO:0090304 GO:0090305 GO:0090407 GO:0090734 GO:0097159 GO:0098687 GO:0098847 GO:0140013 GO:1901293 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1903046
KEGG Pathways
Metabolic & Signaling
ko03440 ko03450 ko04218 map03440 map03450 map04218
Pfam Domains
Protein Families
AAA_23 Rad50_zn_hook

Protein Analysis

1317

Amino Acids

153.32

Weight (kDa)

5.92

Isoelectric Point (pI)

41.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SMC_N PF02463 4 - 105 4.9e-06 RecF/RecN/SMC N terminal domain
AAA_23 PF13476 6 - 215 2.7e-33 AAA domain
AAA_15 PF13175 22 - 330 5.8e-08 AAA ATPase domain
Rad50_zn_hook PF04423 688 - 724 4.7e-06 Rad50 zinc hook motif
SMC_N PF02463 1075 - 1302 5e-09 RecF/RecN/SMC N terminal domain
SbcC_Walker_B PF13558 1190 - 1259 2.9e-06 SbcC/RAD50-like, Walker B motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012062)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G31970
fragaria_vesca FvH4_4g13300
malus_domestica MD05G1230300.v1.1
prunus_persica Prupe.1G165000_v2.0.a1 Prupe.1G165000_v2.0.a1 Prupe.1G165000_v2.0.a1 Prupe.1G165000_v2.0.a1 Prupe.1G165000_v2.0.a1
pyrus_communis pycom04g01350
rosa_chinensis RchiOBHm_Chr4g0413591
rosa_laevigata RLG00000008210
rosa_multiflora Rmu_sc0014097.1_g000002
rosa_roxburghii Rroxscaffold_5G00357470
rosa_rugosa Rorug04G0121100
rosa_samantha Rh4AG180900 Rh4BG180100 Rh4CG192000 Rh4DG176200
rosa_wichuraiana Rw4G015110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 26, 3309
AasI GACNNNNNNGTC 1 cut(s) 209
AatII GACGTC 1 cut(s) 279
AccB1I GGYRCC 1 cut(s) 110
AccB7I CCANNNNNTGG 1 cut(s) 754
AccI GTMKAC 2 cut(s) 557, 1918
AccIII TCCGGA 1 cut(s) 36
AciI CCGC 5 cut(s) 250, 264, 372, 799, 2638
AclWI GGATC 4 cut(s) 500, 603, 2042, 2505
AcoI YGGCCR 6 cut(s) 100, 184, 261, 811, 2724, 3862
AcsI RAATTY 7 cut(s) 765, 945, 966, 1249, 1505, 3030, 3182
AcuI CTGAAG 6 cut(s) 539, 809, 1150, 1284, 1907, 3150
AcyI GRCGYC 2 cut(s) 111, 276
AfaI GTAC 4 cut(s) 7, 2399, 2606, 3356
AfeI AGCGCT 1 cut(s) 3845
AfiI CCNNNNNNNGG 5 cut(s) 177, 371, 754, 1291, 2854
AflII CTTAAG 1 cut(s) 2906
AhdI GACNNNNNGTC 1 cut(s) 234
AjiI CACGTC 1 cut(s) 64
AjnI CCWGG 6 cut(s) 176, 804, 1332, 2500, 3405, 3933
AjuI GAANNNNNNNTTGG 4 cut(s) 1574, 1606, 1625, 1657
AloI GAACNNNNNNTCC 2 cut(s) 2111, 2143
Alw26I GTCTC 6 cut(s) 221, 286, 445, 859, 1629, 1640
AlwI GGATC 4 cut(s) 500, 603, 2042, 2505
AlwNI CAGNNNCTG 1 cut(s) 3551
Ama87I CYCGRG 2 cut(s) 455, 1575
Aor13HI TCCGGA 1 cut(s) 36
Aor51HI AGCGCT 1 cut(s) 3845
ApeKI GCWGC 5 cut(s) 659, 2153, 3499, 3767, 3770
ApoI RAATTY 7 cut(s) 765, 945, 966, 1249, 1505, 3030, 3182
ArsI GACNNNNNNTTYG 4 cut(s) 999, 1031, 3159, 3191
AseI ATTAAT 1 cut(s) 360
Asp700I GAANNNNTTC 1 cut(s) 2760
AspLEI GCGC 6 cut(s) 113, 1799, 3427, 3447, 3846, 3870
AspS9I GGNCC 9 cut(s) 180, 1282, 1552, 2498, 2780, 3403, 3649, 3746, 3793
AsuC2I CCSGG 1 cut(s) 50
AvaI CYCGRG 2 cut(s) 455, 1575
AvaII GGWCC 7 cut(s) 180, 1282, 1552, 3403, 3649, 3746, 3793
BaeGI GKGCMC 1 cut(s) 2074
BalI TGGCCA 2 cut(s) 813, 3864
BanI GGYRCC 1 cut(s) 110
BauI CACGAG 2 cut(s) 1806, 2067
BbsI GAAGAC 4 cut(s) 903, 945, 1640, 2127
BbvCI CCTCAGC 1 cut(s) 1074
BbvI GCAGC 5 cut(s) 646, 2165, 3511, 3754, 3757
BceAI ACGGC 3 cut(s) 124, 276, 1061
BcgI CGANNNNNNTGC 2 cut(s) 259, 293
BciT130I CCWGG 6 cut(s) 178, 806, 1334, 2502, 3407, 3935
BclI TGATCA 4 cut(s) 1560, 3361, 3663, 3907
BcnI CCSGG 1 cut(s) 50
BcoDI GTCTC 6 cut(s) 221, 286, 445, 859, 1629, 1640
BfaI CTAG 8 cut(s) 777, 848, 1223, 2579, 3018, 3614, 3713, 3719
BfmI CTRYAG 1 cut(s) 394
BfoI RGCGCY 4 cut(s) 114, 1800, 3847, 3871
BfrI CTTAAG 1 cut(s) 2906
BglI GCCNNNNNGGC 1 cut(s) 108
BglII AGATCT 2 cut(s) 877, 1885
BisI GCNGC 6 cut(s) 264, 660, 2154, 3500, 3768, 3771
BlpI GCTNAGC 1 cut(s) 1326
BlsI GCNGC 6 cut(s) 265, 661, 2155, 3501, 3769, 3772
BmcAI AGTACT 1 cut(s) 2399
Bme1390I CCNGG 7 cut(s) 50, 178, 806, 1334, 2502, 3407, 3935
Bme18I GGWCC 7 cut(s) 180, 1282, 1552, 3403, 3649, 3746, 3793
BmeRI GACNNNNNGTC 1 cut(s) 234
BmeT110I CYCGRG 2 cut(s) 455, 1575
BmgBI CACGTC 1 cut(s) 64
BmgT120I GGNCC 9 cut(s) 180, 1282, 1552, 2498, 2780, 3403, 3649, 3746, 3793
BmiI GGNNCC 3 cut(s) 112, 803, 1283
BmrFI CCNGG 7 cut(s) 50, 178, 806, 1334, 2502, 3407, 3935
BmsI GCATC 7 cut(s) 9, 646, 714, 802, 1285, 3088, 3432
BpiI GAAGAC 4 cut(s) 903, 945, 1640, 2127
BpmI CTGGAG 1 cut(s) 2094
Bpu10I CCTNAGC 2 cut(s) 389, 1074
Bpu1102I GCTNAGC 1 cut(s) 1326
BpuEI CTTGAG 7 cut(s) 125, 588, 1783, 1813, 2182, 2352, 3132
BpuMI CCSGG 1 cut(s) 50
BsaHI GRCGYC 2 cut(s) 111, 276
BsaI GGTCTC 1 cut(s) 221
BsaJI CCNNGG 4 cut(s) 561, 1776, 2514, 3933
BsaWI WCCGGW 3 cut(s) 36, 3114, 3795
BsaXI ACNNNNNCTCC 4 cut(s) 366, 396, 854, 884
Bsc4I CCNNNNNNNGG 5 cut(s) 177, 371, 754, 1291, 2854
Bse118I RCCGGY 4 cut(s) 113, 155, 215, 266
Bse1I ACTGG 2 cut(s) 2848, 3604
Bse3DI GCAATG 3 cut(s) 681, 1177, 1946
BseAI TCCGGA 1 cut(s) 36
BseBI CCWGG 6 cut(s) 178, 806, 1334, 2502, 3407, 3935
BseDI CCNNGG 4 cut(s) 561, 1776, 2514, 3933
BseGI GGATG 8 cut(s) 195, 814, 997, 1300, 1600, 1612, 3748, 3910
BseLI CCNNNNNNNGG 5 cut(s) 177, 371, 754, 1291, 2854
BseMI GCAATG 3 cut(s) 681, 1177, 1946
BseMII CTCAG 9 cut(s) 320, 1065, 1340, 1664, 1769, 2157, 2295, 3378, 3747
BseNI ACTGG 2 cut(s) 2848, 3604
BseRI GAGGAG 4 cut(s) 1391, 1716, 1915, 2870
BseSI GKGCMC 1 cut(s) 2074
BseX3I CGGCCG 1 cut(s) 261
BseXI GCAGC 5 cut(s) 646, 2165, 3511, 3754, 3757
BseYI CCCAGC 1 cut(s) 3560
BsgI GTGCAG 1 cut(s) 1587
Bsh1285I CGRYCG 2 cut(s) 264, 2845
BshNI GGYRCC 1 cut(s) 110
BsiEI CGRYCG 2 cut(s) 264, 2845
BsiHKCI CYCGRG 2 cut(s) 455, 1575
BsiSI CCGG 9 cut(s) 37, 50, 114, 156, 183, 216, 267, 3115, 3796
BslFI GGGAC 2 cut(s) 1295, 3577
BslI CCNNNNNNNGG 5 cut(s) 177, 371, 754, 1291, 2854
BsmAI GTCTC 6 cut(s) 221, 286, 445, 859, 1629, 1640
BsmBI CGTCTC 1 cut(s) 286
BsmFI GGGAC 2 cut(s) 1295, 3577
BsmI GAATGC 1 cut(s) 3027
Bso31I GGTCTC 1 cut(s) 221
BsoBI CYCGRG 2 cut(s) 455, 1575
Bsp1286I GDGCHC 1 cut(s) 2074
Bsp13I TCCGGA 1 cut(s) 36
Bsp1720I GCTNAGC 1 cut(s) 1326
Bsp19I CCATGG 1 cut(s) 1776
BspACI CCGC 5 cut(s) 250, 264, 372, 799, 2638
BspCNI CTCAG 9 cut(s) 319, 1066, 1339, 1663, 1768, 2158, 2296, 3379, 3748
BspEI TCCGGA 1 cut(s) 36
BspHI TCATGA 1 cut(s) 3835
BspLI GGNNCC 3 cut(s) 112, 803, 1283
BspPI GGATC 4 cut(s) 500, 603, 2042, 2505
BspT107I GGYRCC 1 cut(s) 110
BspTI CTTAAG 1 cut(s) 2906
BspTNI GGTCTC 1 cut(s) 221
BsrDI GCAATG 3 cut(s) 681, 1177, 1946
BsrFI RCCGGY 4 cut(s) 113, 155, 215, 266
BsrI ACTGG 2 cut(s) 2848, 3604
BssAI RCCGGY 4 cut(s) 113, 155, 215, 266
BssECI CCNNGG 4 cut(s) 561, 1776, 2514, 3933
BssNAI GTATAC 1 cut(s) 558
BssNI GRCGYC 2 cut(s) 111, 276
BssSI CACGAG 2 cut(s) 1806, 2067
BssT1I CCWWGG 3 cut(s) 561, 1776, 2514
Bst1107I GTATAC 1 cut(s) 558
Bst2BI CACGAG 2 cut(s) 1806, 2067
Bst2UI CCWGG 6 cut(s) 178, 806, 1334, 2502, 3407, 3935
Bst4CI ACNGT 9 cut(s) 10, 555, 870, 1424, 1623, 1880, 1951, 2648, 3354
Bst6I CTCTTC 7 cut(s) 903, 1155, 1985, 2109, 2310, 2652, 2703
BstACI GRCGYC 2 cut(s) 111, 276
BstAFI CTTAAG 1 cut(s) 2906
BstAPI GCANNNNNTGC 1 cut(s) 830
BstDSI CCRYGG 1 cut(s) 1776
BstF5I GGATG 8 cut(s) 195, 814, 997, 1300, 1600, 1612, 3748, 3910
BstH2I RGCGCY 4 cut(s) 114, 1800, 3847, 3871
BstHHI GCGC 6 cut(s) 113, 1799, 3427, 3447, 3846, 3870
BstMAI GTCTC 6 cut(s) 221, 286, 445, 859, 1629, 1640
BstMCI CGRYCG 2 cut(s) 264, 2845
BstMWI GCNNNNNNNGC 6 cut(s) 108, 830, 2363, 2977, 3373, 3499
BstNI CCWGG 6 cut(s) 178, 806, 1334, 2502, 3407, 3935
BstNSI RCATGY 2 cut(s) 1462, 3878
BstSCI CCNGG 7 cut(s) 48, 176, 804, 1332, 2500, 3405, 3933
BstSFI CTRYAG 1 cut(s) 394
BstSLI GKGCMC 1 cut(s) 2074
BstV1I GCAGC 5 cut(s) 646, 2165, 3511, 3754, 3757
BstV2I GAAGAC 4 cut(s) 903, 945, 1640, 2127
BstX2I RGATCY 4 cut(s) 505, 877, 1885, 2510
BstXI CCANNNNNNTGG 2 cut(s) 568, 1972
BstYI RGATCY 4 cut(s) 505, 877, 1885, 2510
BstZ17I GTATAC 1 cut(s) 558
BstZI CGGCCG 1 cut(s) 261
BtgI CCRYGG 1 cut(s) 1776
BtrI CACGTC 1 cut(s) 64
BtsCI GGATG 8 cut(s) 195, 814, 997, 1300, 1600, 1612, 3748, 3910
BtsI GCAGTG 1 cut(s) 748
BtsIMutI CAGTG 8 cut(s) 515, 748, 785, 900, 1570, 2653, 2855, 3437
CaiI CAGNNNCTG 1 cut(s) 3551
CciI TCATGA 1 cut(s) 3835
CfoI GCGC 6 cut(s) 113, 1799, 3427, 3447, 3846, 3870
Cfr10I RCCGGY 4 cut(s) 113, 155, 215, 266
Cfr13I GGNCC 9 cut(s) 180, 1282, 1552, 2498, 2780, 3403, 3649, 3746, 3793
CseI GACGC 1 cut(s) 334
Csp6I GTAC 4 cut(s) 6, 2398, 2605, 3355
CspCI CAANNNNNGTGG 4 cut(s) 2621, 2656, 3250, 3285
CviQI GTAC 4 cut(s) 6, 2398, 2605, 3355
DinI GGCGCC 1 cut(s) 112
DraI TTTAAA 1 cut(s) 1419
DrdI GACNNNNNNGTC 1 cut(s) 209
DriI GACNNNNNGTC 1 cut(s) 234
DseDI GACNNNNNNGTC 1 cut(s) 209
EaeI YGGCCR 6 cut(s) 100, 184, 261, 811, 2724, 3862
EagI CGGCCG 1 cut(s) 261
Eam1104I CTCTTC 7 cut(s) 903, 1155, 1985, 2109, 2310, 2652, 2703
Eam1105I GACNNNNNGTC 1 cut(s) 234
EarI CTCTTC 7 cut(s) 903, 1155, 1985, 2109, 2310, 2652, 2703
EclXI CGGCCG 1 cut(s) 261
Eco130I CCWWGG 3 cut(s) 561, 1776, 2514
Eco147I AGGCCT 2 cut(s) 1760, 1928
Eco31I GGTCTC 1 cut(s) 221
Eco32I GATATC 1 cut(s) 538
Eco47I GGWCC 7 cut(s) 180, 1282, 1552, 3403, 3649, 3746, 3793
Eco47III AGCGCT 1 cut(s) 3845
Eco52I CGGCCG 1 cut(s) 261
Eco57I CTGAAG 6 cut(s) 539, 809, 1150, 1284, 1907, 3150
Eco88I CYCGRG 2 cut(s) 455, 1575
EcoO109I RGGNCCY 3 cut(s) 1282, 3403, 3649
EcoRI GAATTC 2 cut(s) 945, 1249
EcoRII CCWGG 6 cut(s) 176, 804, 1332, 2500, 3405, 3933
EcoRV GATATC 1 cut(s) 538
EcoT14I CCWWGG 3 cut(s) 561, 1776, 2514
EgeI GGCGCC 1 cut(s) 112
EheI GGCGCC 1 cut(s) 112
ErhI CCWWGG 3 cut(s) 561, 1776, 2514
Esp3I CGTCTC 1 cut(s) 286
FaqI GGGAC 2 cut(s) 1295, 3577
FbaI TGATCA 4 cut(s) 1560, 3361, 3663, 3907
FblI GTMKAC 2 cut(s) 557, 1918
Fnu4HI GCNGC 6 cut(s) 264, 660, 2154, 3500, 3768, 3771
FokI GGATG 8 cut(s) 182, 821, 1004, 1307, 1607, 1619, 3755, 3917
Fsp4HI GCNGC 6 cut(s) 264, 660, 2154, 3500, 3768, 3771
FspBI CTAG 8 cut(s) 777, 848, 1223, 2579, 3018, 3614, 3713, 3719
GlaI GCGC 6 cut(s) 112, 1798, 3426, 3446, 3845, 3869
GluI GCNGC 6 cut(s) 264, 660, 2154, 3500, 3768, 3771
GsaI CCCAGC 1 cut(s) 3564
GsuI CTGGAG 1 cut(s) 2094
HaeII RGCGCY 4 cut(s) 114, 1800, 3847, 3871
HapII CCGG 9 cut(s) 37, 50, 114, 156, 183, 216, 267, 3115, 3796
HgaI GACGC 1 cut(s) 334
HhaI GCGC 6 cut(s) 113, 1799, 3427, 3447, 3846, 3870
Hin1I GRCGYC 2 cut(s) 111, 276
Hin6I GCGC 6 cut(s) 111, 1797, 3425, 3445, 3844, 3868
HinP1I GCGC 6 cut(s) 111, 1797, 3425, 3445, 3844, 3868
HincII GTYRAC 1 cut(s) 1474
HindII GTYRAC 1 cut(s) 1474
HindIII AAGCTT 2 cut(s) 2231, 2483
HpaII CCGG 9 cut(s) 37, 50, 114, 156, 183, 216, 267, 3115, 3796
Hpy166II GTNNAC 8 cut(s) 13, 558, 1474, 1874, 1919, 2096, 2227, 3286
Hpy8I GTNNAC 8 cut(s) 13, 558, 1474, 1874, 1919, 2096, 2227, 3286
Hpy99I CGWCG 2 cut(s) 101, 281
HpyCH4III ACNGT 9 cut(s) 10, 555, 870, 1424, 1623, 1880, 1951, 2648, 3354
HpyCH4IV ACGT 6 cut(s) 63, 276, 932, 1587, 2463, 2983
HpyF10VI GCNNNNNNNGC 6 cut(s) 108, 830, 2363, 2977, 3373, 3499
HpySE526I ACGT 6 cut(s) 63, 276, 932, 1587, 2463, 2983
Hsp92I GRCGYC 2 cut(s) 111, 276
HspAI GCGC 6 cut(s) 111, 1797, 3425, 3445, 3844, 3868
KasI GGCGCC 1 cut(s) 110
Kpn2I TCCGGA 1 cut(s) 36
KroI GCCGGC 3 cut(s) 113, 215, 266
KroNI GCCGGC 3 cut(s) 115, 217, 268
Ksp22I TGATCA 4 cut(s) 1560, 3361, 3663, 3907
LmnI GCTCC 5 cut(s) 39, 252, 1390, 2857, 3490
Lsp1109I GCAGC 5 cut(s) 646, 2165, 3511, 3754, 3757
LweI GCATC 7 cut(s) 9, 646, 714, 802, 1285, 3088, 3432
MaeI CTAG 8 cut(s) 777, 848, 1223, 2579, 3018, 3614, 3713, 3719
MaeII ACGT 6 cut(s) 63, 276, 932, 1587, 2463, 2983
MaeIII GTNAC 5 cut(s) 1424, 1570, 1771, 2958, 3895
MfeI CAATTG 1 cut(s) 3700
MflI RGATCY 4 cut(s) 505, 877, 1885, 2510
MhlI GDGCHC 1 cut(s) 2074
MlsI TGGCCA 2 cut(s) 813, 3864
MluNI TGGCCA 2 cut(s) 813, 3864
Mly113I GGCGCC 1 cut(s) 111
MlyI GAGTC 5 cut(s) 1642, 2201, 2746, 3559, 3769
MmeI TCCRAC 2 cut(s) 714, 1289
Mox20I TGGCCA 2 cut(s) 813, 3864
MreI CGCCGGCG 1 cut(s) 215
MroI TCCGGA 1 cut(s) 36
MroNI GCCGGC 3 cut(s) 113, 215, 266
MroXI GAANNNNTTC 1 cut(s) 2760
MscI TGGCCA 2 cut(s) 813, 3864
MslI CAYNNNNRTG 2 cut(s) 2633, 2678
Msp20I TGGCCA 2 cut(s) 813, 3864
MspA1I CMGCKG 1 cut(s) 372
MspCI CTTAAG 1 cut(s) 2906
MspI CCGG 9 cut(s) 37, 50, 114, 156, 183, 216, 267, 3115, 3796
MspR9I CCNGG 7 cut(s) 50, 178, 806, 1334, 2502, 3407, 3935
MunI CAATTG 1 cut(s) 3700
Mva1269I GAATGC 1 cut(s) 3027
MvaI CCWGG 6 cut(s) 178, 806, 1334, 2502, 3407, 3935
MwoI GCNNNNNNNGC 6 cut(s) 108, 830, 2363, 2977, 3373, 3499
NaeI GCCGGC 3 cut(s) 115, 217, 268
NarI GGCGCC 1 cut(s) 111
NciI CCSGG 1 cut(s) 50
NcoI CCATGG 1 cut(s) 1776
NgoMIV GCCGGC 3 cut(s) 113, 215, 266
NlaIV GGNNCC 3 cut(s) 112, 803, 1283
NmuCI GTSAC 1 cut(s) 3895
NspI RCATGY 2 cut(s) 1462, 3878
PaeI GCATGC 1 cut(s) 3878
PaeR7I CTCGAG 2 cut(s) 455, 1575
PagI TCATGA 1 cut(s) 3835
PceI AGGCCT 2 cut(s) 1760, 1928
PcsI WCGNNNNNNNCGW 1 cut(s) 3162
PctI GAATGC 1 cut(s) 3027
PdiI GCCGGC 3 cut(s) 115, 217, 268
PdmI GAANNNNTTC 1 cut(s) 2760
PflFI GACNNNGTC 1 cut(s) 278
PflMI CCANNNNNTGG 1 cut(s) 754
PkrI GCNGC 6 cut(s) 265, 661, 2155, 3501, 3769, 3772
PleI GAGTC 5 cut(s) 1642, 2201, 2745, 3559, 3768
PluTI GGCGCC 1 cut(s) 114
PpsI GAGTC 5 cut(s) 1642, 2201, 2745, 3559, 3768
PpuMI RGGWCCY 3 cut(s) 1282, 3403, 3649
PshBI ATTAAT 1 cut(s) 360
PsiI TTATAA 2 cut(s) 26, 3309
Psp5II RGGWCCY 3 cut(s) 1282, 3403, 3649
Psp6I CCWGG 6 cut(s) 176, 804, 1332, 2500, 3405, 3933
PspFI CCCAGC 1 cut(s) 3560
PspGI CCWGG 6 cut(s) 176, 804, 1332, 2500, 3405, 3933
PspN4I GGNNCC 3 cut(s) 112, 803, 1283
PspPI GGNCC 9 cut(s) 180, 1282, 1552, 2498, 2780, 3403, 3649, 3746, 3793
PspPPI RGGWCCY 3 cut(s) 1282, 3403, 3649
PstNI CAGNNNCTG 1 cut(s) 3551
PsuI RGATCY 4 cut(s) 505, 877, 1885, 2510
PsyI GACNNNGTC 1 cut(s) 278
RsaI GTAC 4 cut(s) 7, 2399, 2606, 3356
RsaNI GTAC 4 cut(s) 6, 2398, 2605, 3355
RseI CAYNNNNRTG 2 cut(s) 2633, 2678
SatI GCNGC 6 cut(s) 264, 660, 2154, 3500, 3768, 3771
Sau96I GGNCC 9 cut(s) 180, 1282, 1552, 2498, 2780, 3403, 3649, 3746, 3793
ScaI AGTACT 1 cut(s) 2399
SchI GAGTC 5 cut(s) 1642, 2201, 2746, 3559, 3769
ScrFI CCNGG 7 cut(s) 50, 178, 806, 1334, 2502, 3407, 3935
SduI GDGCHC 1 cut(s) 2074
SfaNI GCATC 7 cut(s) 9, 646, 714, 802, 1285, 3088, 3432
SfcI CTRYAG 1 cut(s) 394
SfoI GGCGCC 1 cut(s) 112
Sfr274I CTCGAG 2 cut(s) 455, 1575
SgrAI CRCCGGYG 2 cut(s) 155, 215
SinI GGWCC 7 cut(s) 180, 1282, 1552, 3403, 3649, 3746, 3793
SlaI CTCGAG 2 cut(s) 455, 1575
SmiMI CAYNNNNRTG 2 cut(s) 2633, 2678
SphI GCATGC 1 cut(s) 3878
SseBI AGGCCT 2 cut(s) 1760, 1928
SsiI CCGC 5 cut(s) 250, 264, 372, 799, 2638
SspDI GGCGCC 1 cut(s) 110
SspI AATATT 2 cut(s) 3298, 3887
SspMI CTAG 8 cut(s) 777, 848, 1223, 2579, 3018, 3614, 3713, 3719
StuI AGGCCT 2 cut(s) 1760, 1928
StyD4I CCNGG 7 cut(s) 48, 176, 804, 1332, 2500, 3405, 3933
StyI CCWWGG 3 cut(s) 561, 1776, 2514
TaaI ACNGT 9 cut(s) 10, 555, 870, 1424, 1623, 1880, 1951, 2648, 3354
TaiI ACGT 6 cut(s) 66, 279, 935, 1590, 2466, 2986
TaqI TCGA 8 cut(s) 132, 339, 456, 949, 1576, 2751, 3165, 3455
TaqII GACCGA 1 cut(s) 2831
TatI WGTACW 1 cut(s) 2397
TauI GCSGC 1 cut(s) 266
TscAI CASTG 8 cut(s) 522, 748, 792, 907, 1570, 2653, 2855, 3444
TseFI GTSAC 1 cut(s) 3895
TseI GCWGC 5 cut(s) 659, 2153, 3499, 3767, 3770
Tsp45I GTSAC 1 cut(s) 3895
TspRI CASTG 8 cut(s) 522, 748, 792, 907, 1570, 2653, 2855, 3444
Tth111I GACNNNGTC 1 cut(s) 278
Van91I CCANNNNNTGG 1 cut(s) 754
Vha464I CTTAAG 1 cut(s) 2906
VpaK11BI GGWCC 7 cut(s) 180, 1282, 1552, 3403, 3649, 3746, 3793
VspI ATTAAT 1 cut(s) 360
XapI RAATTY 7 cut(s) 765, 945, 966, 1249, 1505, 3030, 3182
XbaI TCTAGA 2 cut(s) 1222, 2578
XceI RCATGY 2 cut(s) 1462, 3878
XhoI CTCGAG 2 cut(s) 455, 1575
XmiI GTMKAC 2 cut(s) 557, 1918
XmnI GAANNNNTTC 1 cut(s) 2760
XspI CTAG 8 cut(s) 777, 848, 1223, 2579, 3018, 3614, 3713, 3719
ZraI GACGTC 1 cut(s) 277
ZrmI AGTACT 1 cut(s) 2399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.