MD05G1230300.v1.1

DNA repair protein RAD50

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0012062
Physical Location & Seq
Reverse (-)
36369225 .. 36382375
13151 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1230300.v1.1.491

Sequence Viewer

Length: 3948 bp
ATGAGTACGGTGGACAAGATGCTAATAAAGGGAATCCGGAGCTTCGACCCGGAGAACAAGCACGTCATCACCTTCTTCAAGCCCTTAACCCTAATCGTCGGCCCCAACGGCGCCGGCAAAACCACCATCATCGAGTGCTTGAAGCTCTCCTGCTCCGGCGAGTTGCCTCCCAACGCTCGCTCCGGCCACAGCTTCGTCCACGACCCTAAGGTCGCCGGTGAAACTGAGACCAAAGCCCAGATTAAGCTCCGCTTCAGGACCGCCGCCGGCAAAGATGTGGTGTGCATTAGGTCGTTTCAGCTCACGCAGAAAGCCTCGAAAATGGAGTTCAAGGCCATCGACAGCGTCCTTCAGACCATTAATCCCCACACTGGAGAGAAAGTTTGCCTTAGTTATCGATGTGCCGATATGGATAGGGAAATCCCTGCTTTAATGGGTGTATCAAAGGCTATCTTGGAAAATGTTATATTTGTGCACCAAGATGAAGCCAATTGGCCTTTGCAGGATCCTTCAACATTGAAGAAGAAGTTTGATGATATCTTCTCTGCTACCCGATATACTAAGGCATTGGAGGTAATTAAAAAACTTCACAAGGATCAAGCTCAAGAGATAAAGACCTACAAGCTACAGTTGGAGAATCTTCAAACCAAAAAAGATATTGCATATAAGCTTCGCGAGCAAATTTCTCAAGCTCAAGAAAAAACAAATATTTTGAAAAGCGAGATGCAGGGGTTGGAGGGAATGATCCAAGATGTGAATACTAAAATTCAGTATACTGACACGAAACTAAAGGATTTGAAGAAGCTGGAGGACCAGATATCACAGAAAAGTGCTGTGAGAAGCACCCTGGTTAACGAGCGGGAGGAGAAGCACAAAGCTCTTGAAGAGGAGAACGAAGACACTGACGAAGAATTGAAGGAATGGAAAACCAAGTTTGAAGAAAAGATTGCGCTTTTGGAAACTAAAATCAGGAAGCTGGAGAGGGAAGAGAATGACGCAGAGAGCAAAATTACTTTCCTTGAAAAAACGCTAAAGGCATCTATATGGGAGATTAGCAAGCTCGAAAACGAAGCTGAAGTTCATTTGTGCTCAAAGAATGAGAGAGATTCTGTCATTCAAAAACTCTTTTCAAGGCACAATTTGGGTGCTCTTCCAAATCCTCCCTTCAGCAATGAAGTTGCACTGAACCTCACCAATCGAATAAAATCAAGATTGTTGGATCTAGAAAAGGATTTGCAAGATAAGAAGAGGTCAAATGAAAAAGAACTTGAGACAACATGGGACTGCTACAATGATGCAAAAGACCATTGGAAGTCTATTGAGGCTCAGAAAGAAGCTAAAGAAGAAATAAGGAATGGCCTTCTGAAGCGCATAGAGGAAAAAAAGAATGAGCGTGATTCTTTAGAACTTGAAGTTTCAAATGTTGATCTCTCTCACATTGATGAAAAAGAAAAGAATATGCATATTGAGTTTGAGAGAAAGACAAACCAGTATGCTGAAAGGGAATTTGAATCAACAATACGCCAAAAGGATAATGAGATGTTTAGCATTGAGCAAATGATTACAGTTGCTAAGAAAGAGAAAGCTGCCATGGCTGTTGATATGGAGGACAGAACAAAACTGTCTATGAAGAAGAATGATTTGGAGAATCGTAAAAGGCAACATAGAAAGATTATTAATGAAAACAGGGAGAGAGTTAGAGGAGTGCTAAAAGGGAGGTTTCCTCCAGAGAAGGATTTGAGAGGCGAAATCAGTCAAGTCTTGAGTGCTGTTACCATGGAGTTTGATGACTTGAGCACCAAATCTCGTGAAGCGGAGAATGAGGTGAATATGTTGCAGACAAGAATACAAGAAATTAACAATAACATCTACAAGCATCGGAAAGATATGGACTCAAAGAGGAGATATATTGAGTCTAAACTTCAGGCCTTAGATCAGCAATCTTTTACTGTTGATTACTATCCTACAGTCTTGGATTCAGCCAAGGAGAAAAGAGATGTCGAGAAAAGGAAATACAATTTTGCTGATGGTATGCGACAAATGTTTGATCCATTTGAAAGAGTTGCCCGTGCCAATCATATTTGCCCTTGCTGTGAACGACCTTTTTCTCTACAAGAGGAAGACGAATTTGTTAAAAAGCAAAGAATGAATGCTGCAAGTTCTGCTGAGAAATTAAAAGTGCTGGCTGCAGAGTCCTCGACTGCTGACTCTTTTTTTCAGCAATTGGACAGGCTTCGCATGGTTTATGAAGAATATGTTAACATTGGGAAGGAGAGAATCCCCAATGCAGAGAGAGAACTGGATGACCTTACTCAAGAGATGGAACAAAAGTCTCAGGCCCTTGATGATGTGTTAGCTGTTTCGGCTCAAGTAAAGGCTGAAAAGGACTCGATTCAGGCCCTGATGGAACCCATTGAAAATGCTGACAGACTATTTCAAGATATACAAAGAGAACAGAAGCTAGTTGATGAGCTGGCACATAAACTTGAACAAGGACATGGTTCGAGATCTATGCAAGATATTGAGTCGGAGCTGGATAATCTGCTTGAGACAAAGGATAATTTGCATAGAGAAGTAATGAAGTTGAGAAGCGAACAAATGGACCTGCAAGATGATTTGAGCACGGTCAAAACTCGGTGGCACAACGTAAGGGAAGAGAAAATCAAAGCAGCTAATTTATTGTCCGATGTCAAAAGGGCAGAAGAAGAGTTGGAGCGTTTGACCGAAGAAAAGAATCGAGTTGATCTTGATGAAAAGCTTTTGGCAGAGTCTCTTCGCCCTTTATCGAAGGAGAAAGATAAATTACTTGGCTACCACAATGACTTAAAAGTTAAACTTAATCGCGAATATGATGAGCAAGCTGAACGAAAAAGAGAATACGAGCAGGAAGTTGAGGCATTAAATATGATTAATTCGAAAATTAAAGAGTATAATGATTCAAAGAAAGGTGAGAAGTTGCAGGAAATCCAAGAAAAGCAACATCTGCTTGAATCTCAACTTCAAAGTTGTAAAACTAGAAAGAAGGAAATCTTAGCTGAGTTGAACAAAAGCAAAGATTTAAGAGCGAATCAAGATCAGGTAAGACGAAATATTGAGGATAATTTGAGTTACAGGAAAACGAAAGCTGAAGTAGAGGAACTTACCTGTGAGCTTGAGTCACTGGAAGACAAAATGCTGAAGATTGGTCGGAAGCCCAAAGTTCAAGCTGAACTTGAAAAGCTCTCTCATGAGAGGGAGGAGTTTCTGAAAAGGTTAAACCAGAATGATGGGATCATTTCTGCTCACCAGAGCGAAATTTCAAAGCATAAAATTGATCTCAAAAACCCAGATTACAAGGACATTGACAAGCGGTACTTTGATCAGCTAATCCAGCTAAAGACAACTGAGATGGCAAACAAGGACCTGGACAGATACTACAATGCGCTTGACAAAGCATTAATGCGCTTCCACAGCATGAAGATGGAGGAAATAAATAAAATTATAAGAGAGTTGTGGCAGCAAACATACAGAGGACAAGATATAGACTACATCTGTATACACTCAGATTCTGATGGTGCTGGGACTCGTTCTTACAGTTACAAGGTTCTCATGCAGACTGGTGATACAGAGCTTGAAATGAGAGGAAGGTGTAGTGCTGGTCAGAAGGTCCTTGCCTCCCTCATCATAAGATTGGCTTTGGCTGAAACTTTCTGCCTCAACTGCGGAATACTAGCACTGGATGAACCAACTACAAACTTGGATGGCCCGAATGCTGATAGTCTTGCCGCTGCTCTCCATAGAATTATGGAGGACCGGAAGGGCCAGGAGAACTTTCAGCTGATAGTAATTACCCATGATGAGCGTTTCGCTCAACTAATTGGCCAGCGCCAGCATGCAGAAAAATATTATCGGGTGGCGAAGGATGATCACCAGCATAGCATAATCGAAGCCCAGGAGATCTTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000228 GO:0000280 GO:0000722 GO:0000723 GO:0000724 GO:0000725 GO:0000781 GO:0000784 GO:0000785 GO:0000790 GO:0000793 GO:0000794 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003691 GO:0003697 GO:0003824 GO:0004017 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006278 GO:0006281 GO:0006302 GO:0006310 GO:0006312 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007004 GO:0007049 GO:0007127 GO:0007131 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009117 GO:0009123 GO:0009165 GO:0009987 GO:0010833 GO:0016043 GO:0016233 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016776 GO:0018130 GO:0019205 GO:0019438 GO:0019637 GO:0022402 GO:0022414 GO:0030870 GO:0031974 GO:0031981 GO:0032200 GO:0032392 GO:0032508 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0035825 GO:0035861 GO:0042162 GO:0042592 GO:0043047 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0046940 GO:0048285 GO:0050145 GO:0050896 GO:0051276 GO:0051321 GO:0051716 GO:0051880 GO:0055086 GO:0060249 GO:0061982 GO:0065007 GO:0065008 GO:0070013 GO:0070192 GO:0071103 GO:0071704 GO:0071840 GO:0071897 GO:0090304 GO:0090305 GO:0090407 GO:0090734 GO:0097159 GO:0098687 GO:0098847 GO:0140013 GO:1901293 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1903046
KEGG Pathways
Metabolic & Signaling
ko03440 ko03450 ko04218 map03440 map03450 map04218
Pfam Domains
Protein Families
AAA_23 Rad50_zn_hook

Protein Analysis

1316

Amino Acids

153.47

Weight (kDa)

5.89

Isoelectric Point (pI)

43.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SMC_N PF02463 4 - 91 5.4e-06 RecF/RecN/SMC N terminal domain
AAA_23 PF13476 6 - 234 2e-36 AAA domain
AAA_15 PF13175 22 - 335 1.2e-08 AAA ATPase domain
SbcC_Walker_B PF13558 1188 - 1259 3.2e-06 SbcC/RAD50-like, Walker B motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012062)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G31970
fragaria_vesca FvH4_4g13300
malus_domestica MD05G1230300.v1.1
prunus_persica Prupe.1G165000_v2.0.a1 Prupe.1G165000_v2.0.a1 Prupe.1G165000_v2.0.a1 Prupe.1G165000_v2.0.a1 Prupe.1G165000_v2.0.a1
pyrus_communis pycom04g01350
rosa_chinensis RchiOBHm_Chr4g0413591
rosa_laevigata RLG00000008210
rosa_multiflora Rmu_sc0014097.1_g000002
rosa_roxburghii Rroxscaffold_5G00357470
rosa_rugosa Rorug04G0121100
rosa_samantha Rh4AG180900 Rh4BG180100 Rh4CG192000 Rh4DG176200
rosa_wichuraiana Rw4G015110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3482
AasI GACNNNNNNGTC 1 cut(s) 209
Acc36I ACCTGC 1 cut(s) 2613
AccB1I GGYRCC 1 cut(s) 110
AccBSI CCGCTC 1 cut(s) 859
AccI GTMKAC 2 cut(s) 773, 3535
AccII CGCG 2 cut(s) 675, 2844
AccIII TCCGGA 1 cut(s) 36
AciI CCGC 8 cut(s) 250, 261, 264, 859, 1814, 3349, 3702, 3765
AclWI GGATC 7 cut(s) 500, 513, 603, 739, 1227, 2042, 3278
AcoI YGGCCR 2 cut(s) 184, 3859
AcsI RAATTY 5 cut(s) 681, 765, 1505, 2126, 3294
AcuI CTGAAG 8 cut(s) 238, 335, 1095, 1150, 1385, 1907, 3147, 3197
AcyI GRCGYC 1 cut(s) 111
AfaI GTAC 2 cut(s) 7, 3353
AfiI CCNNNNNNNGG 1 cut(s) 371
AjiI CACGTC 1 cut(s) 64
AjnI CCWGG 4 cut(s) 846, 3402, 3801, 3930
AjuI GAANNNNNNNTTGG 2 cut(s) 1625, 1657
Alw21I GWGCWC 5 cut(s) 477, 1091, 1150, 1799, 2624
Alw26I GTCTC 5 cut(s) 221, 1265, 2337, 2543, 2775
Alw44I GTGCAC 1 cut(s) 473
AlwI GGATC 7 cut(s) 500, 513, 603, 739, 1227, 2042, 3278
AlwNI CAGNNNCTG 2 cut(s) 2401, 3548
Aor13HI TCCGGA 1 cut(s) 36
ApaLI GTGCAC 1 cut(s) 473
ApeKI GCWGC 6 cut(s) 1586, 2153, 2186, 2669, 3496, 3767
ApoI RAATTY 5 cut(s) 681, 765, 1505, 2126, 3294
AseI ATTAAT 4 cut(s) 360, 1677, 2910, 3437
AspLEI GCGC 6 cut(s) 113, 952, 1371, 3424, 3444, 3867
AsuC2I CCSGG 1 cut(s) 50
AsuHPI GGTGA 8 cut(s) 61, 230, 1183, 1837, 2960, 3275, 3611, 3899
AsuII TTCGAA 1 cut(s) 2915
AvaII GGWCC 6 cut(s) 258, 811, 2602, 3400, 3646, 3790
AxyI CCTNAGG 1 cut(s) 207
BaeGI GKGCMC 1 cut(s) 477
BalI TGGCCA 1 cut(s) 3861
BamHI GGATCC 1 cut(s) 505
BanI GGYRCC 1 cut(s) 110
BauI CACGAG 1 cut(s) 1806
BbsI GAAGAC 3 cut(s) 903, 2127, 3171
Bbv12I GWGCWC 5 cut(s) 477, 1091, 1150, 1799, 2624
BbvI GCAGC 6 cut(s) 1573, 2140, 2173, 2681, 3508, 3754
BceAI ACGGC 1 cut(s) 124
BcgI CGANNNNNNTGC 4 cut(s) 2178, 2212, 2494, 2528
BciT130I CCWGG 4 cut(s) 848, 3404, 3803, 3932
BclI TGATCA 2 cut(s) 3358, 3904
BcnI CCSGG 1 cut(s) 50
BcoDI GTCTC 5 cut(s) 221, 1265, 2337, 2543, 2775
BfaI CTAG 4 cut(s) 1223, 2462, 3015, 3710
BfmI CTRYAG 3 cut(s) 626, 1965, 2187
BfoI RGCGCY 2 cut(s) 114, 3868
BfuAI ACCTGC 1 cut(s) 2613
BglI GCCNNNNNGGC 1 cut(s) 108
BglII AGATCT 2 cut(s) 2507, 3936
BisI GCNGC 8 cut(s) 264, 1587, 2154, 2187, 2670, 3497, 3765, 3768
BlsI GCNGC 8 cut(s) 265, 1588, 2155, 2188, 2671, 3498, 3766, 3769
Bme1390I CCNGG 5 cut(s) 50, 848, 3404, 3803, 3932
Bme18I GGWCC 6 cut(s) 258, 811, 2602, 3400, 3646, 3790
BmgBI CACGTC 1 cut(s) 64
BmiI GGNNCC 4 cut(s) 103, 112, 507, 2409
BmrFI CCNGG 5 cut(s) 50, 848, 3404, 3803, 3932
BmsI GCATC 5 cut(s) 9, 714, 1046, 1285, 1885
BpiI GAAGAC 3 cut(s) 903, 2127, 3171
BplI GAGNNNNNCTC 2 cut(s) 1708, 1740
BpmI CTGGAG 4 cut(s) 393, 827, 998, 1710
Bpu14I TTCGAA 1 cut(s) 2915
BpuMI CCSGG 1 cut(s) 50
Bsa29I ATCGAT 1 cut(s) 397
BsaBI GATNNNNATC 1 cut(s) 1959
BsaHI GRCGYC 1 cut(s) 111
BsaI GGTCTC 1 cut(s) 221
BsaJI CCNNGG 5 cut(s) 846, 1590, 1776, 1983, 3930
BsaWI WCCGGW 2 cut(s) 36, 3792
BsaXI ACNNNNNCTCC 4 cut(s) 164, 194, 366, 396
Bsc4I CCNNNNNNNGG 1 cut(s) 371
Bse118I RCCGGY 3 cut(s) 113, 215, 266
Bse1I ACTGG 6 cut(s) 376, 1489, 2304, 3165, 3601, 3720
Bse21I CCTNAGG 1 cut(s) 207
Bse3DI GCAATG 1 cut(s) 1177
Bse8I GATNNNNATC 1 cut(s) 1959
BseAI TCCGGA 1 cut(s) 36
BseBI CCWGG 4 cut(s) 848, 3404, 3803, 3932
BseCI ATCGAT 1 cut(s) 397
BseDI CCNNGG 5 cut(s) 846, 1590, 1776, 1983, 3930
BseGI GGATG 4 cut(s) 2308, 3724, 3745, 3907
BseJI GATNNNNATC 1 cut(s) 1959
BseLI CCNNNNNNNGG 1 cut(s) 371
BseMI GCAATG 1 cut(s) 1177
BseMII CTCAG 7 cut(s) 216, 1340, 2157, 2348, 3027, 3375, 3555
BseNI ACTGG 6 cut(s) 376, 1489, 2304, 3165, 3601, 3720
BseRI GAGGAG 5 cut(s) 878, 902, 1716, 1915, 3251
BseSI GKGCMC 1 cut(s) 477
BseXI GCAGC 6 cut(s) 1573, 2140, 2173, 2681, 3508, 3754
BseYI CCCAGC 1 cut(s) 3557
Bsh1236I CGCG 2 cut(s) 675, 2844
BshNI GGYRCC 1 cut(s) 110
BshVI ATCGAT 1 cut(s) 397
BsiHKAI GWGCWC 5 cut(s) 477, 1091, 1150, 1799, 2624
BsiSI CCGG 8 cut(s) 37, 50, 114, 156, 183, 216, 267, 3793
BslFI GGGAC 2 cut(s) 1295, 3574
BslI CCNNNNNNNGG 1 cut(s) 371
BsmAI GTCTC 5 cut(s) 221, 1265, 2337, 2543, 2775
BsmFI GGGAC 2 cut(s) 1295, 3574
BsmI GAATGC 2 cut(s) 2155, 3754
Bso31I GGTCTC 1 cut(s) 221
Bsp119I TTCGAA 1 cut(s) 2915
Bsp1286I GDGCHC 5 cut(s) 477, 1091, 1150, 1799, 2624
Bsp13I TCCGGA 1 cut(s) 36
Bsp19I CCATGG 2 cut(s) 1590, 1776
Bsp68I TCGCGA 2 cut(s) 675, 2844
BspACI CCGC 8 cut(s) 250, 261, 264, 859, 1814, 3349, 3702, 3765
BspCNI CTCAG 7 cut(s) 217, 1339, 2158, 2347, 3028, 3376, 3554
BspDI ATCGAT 1 cut(s) 397
BspEI TCCGGA 1 cut(s) 36
BspFNI CGCG 2 cut(s) 675, 2844
BspHI TCATGA 1 cut(s) 3226
BspLI GGNNCC 4 cut(s) 103, 112, 507, 2409
BspMAI CTGCAG 1 cut(s) 2191
BspMI ACCTGC 1 cut(s) 2613
BspPI GGATC 7 cut(s) 500, 513, 603, 739, 1227, 2042, 3278
BspQI GCTCTTC 1 cut(s) 1155
BspT104I TTCGAA 1 cut(s) 2915
BspT107I GGYRCC 1 cut(s) 110
BspTNI GGTCTC 1 cut(s) 221
BsrBI CCGCTC 1 cut(s) 859
BsrDI GCAATG 1 cut(s) 1177
BsrFI RCCGGY 3 cut(s) 113, 215, 266
BsrI ACTGG 6 cut(s) 376, 1489, 2304, 3165, 3601, 3720
BssAI RCCGGY 3 cut(s) 113, 215, 266
BssECI CCNNGG 5 cut(s) 846, 1590, 1776, 1983, 3930
BssNAI GTATAC 2 cut(s) 774, 3536
BssNI GRCGYC 1 cut(s) 111
BssSI CACGAG 1 cut(s) 1806
BssT1I CCWWGG 3 cut(s) 1590, 1776, 1983
Bst1107I GTATAC 2 cut(s) 774, 3536
Bst2BI CACGAG 1 cut(s) 1806
Bst2UI CCWGG 4 cut(s) 848, 3404, 3803, 3932
Bst4CI ACNGT 8 cut(s) 10, 630, 1567, 1623, 1951, 1969, 2626, 3575
Bst6I CTCTTC 7 cut(s) 879, 981, 1155, 1241, 2649, 2700, 2778
BstACI GRCGYC 1 cut(s) 111
BstAPI GCANNNNNTGC 2 cut(s) 2162, 2983
BstBI TTCGAA 1 cut(s) 2915
BstDSI CCRYGG 2 cut(s) 1590, 1776
BstF5I GGATG 4 cut(s) 2308, 3724, 3745, 3907
BstFNI CGCG 2 cut(s) 675, 2844
BstH2I RGCGCY 2 cut(s) 114, 3868
BstHHI GCGC 6 cut(s) 113, 952, 1371, 3424, 3444, 3867
BstMAI GTCTC 5 cut(s) 221, 1265, 2337, 2543, 2775
BstMWI GCNNNNNNNGC 9 cut(s) 108, 676, 1592, 2162, 2363, 2983, 3370, 3450, 3699
BstNI CCWGG 4 cut(s) 848, 3404, 3803, 3932
BstNSI RCATGY 1 cut(s) 3875
BstSCI CCNGG 5 cut(s) 48, 846, 3402, 3801, 3930
BstSFI CTRYAG 3 cut(s) 626, 1965, 2187
BstSLI GKGCMC 1 cut(s) 477
BstUI CGCG 2 cut(s) 675, 2844
BstV1I GCAGC 6 cut(s) 1573, 2140, 2173, 2681, 3508, 3754
BstV2I GAAGAC 3 cut(s) 903, 2127, 3171
BstX2I RGATCY 4 cut(s) 505, 1219, 2507, 3936
BstXI CCANNNNNNTGG 1 cut(s) 3266
BstYI RGATCY 4 cut(s) 505, 1219, 2507, 3936
BstZ17I GTATAC 2 cut(s) 774, 3536
Bsu15I ATCGAT 1 cut(s) 397
Bsu36I CCTNAGG 1 cut(s) 207
BsuTUI ATCGAT 1 cut(s) 397
BtgI CCRYGG 2 cut(s) 1590, 1776
BtrI CACGTC 1 cut(s) 64
BtsCI GGATG 4 cut(s) 2308, 3724, 3745, 3907
BtsIMutI CAGTG 5 cut(s) 369, 900, 1181, 3158, 3713
BtuMI TCGCGA 2 cut(s) 675, 2844
BveI ACCTGC 1 cut(s) 2613
CaiI CAGNNNCTG 2 cut(s) 2401, 3548
CciI TCATGA 1 cut(s) 3226
CfoI GCGC 6 cut(s) 113, 952, 1371, 3424, 3444, 3867
Cfr10I RCCGGY 3 cut(s) 113, 215, 266
ClaI ATCGAT 1 cut(s) 397
CseI GACGC 2 cut(s) 334, 1004
Csp6I GTAC 2 cut(s) 6, 3352
CviQI GTAC 2 cut(s) 6, 3352
DinI GGCGCC 1 cut(s) 112
DrdI GACNNNNNNGTC 1 cut(s) 209
DseDI GACNNNNNNGTC 1 cut(s) 209
EaeI YGGCCR 2 cut(s) 184, 3859
Eam1104I CTCTTC 7 cut(s) 879, 981, 1155, 1241, 2649, 2700, 2778
EarI CTCTTC 7 cut(s) 879, 981, 1155, 1241, 2649, 2700, 2778
Eco130I CCWWGG 3 cut(s) 1590, 1776, 1983
Eco147I AGGCCT 1 cut(s) 1928
Eco31I GGTCTC 1 cut(s) 221
Eco32I GATATC 2 cut(s) 538, 819
Eco47I GGWCC 6 cut(s) 258, 811, 2602, 3400, 3646, 3790
Eco57I CTGAAG 8 cut(s) 238, 335, 1095, 1150, 1385, 1907, 3147, 3197
Eco81I CCTNAGG 1 cut(s) 207
EcoO109I RGGNCCY 4 cut(s) 2338, 2398, 3400, 3646
EcoRII CCWGG 4 cut(s) 846, 3402, 3801, 3930
EcoRV GATATC 2 cut(s) 538, 819
EcoT14I CCWWGG 3 cut(s) 1590, 1776, 1983
EcoT22I ATGCAT 1 cut(s) 1464
EgeI GGCGCC 1 cut(s) 112
EheI GGCGCC 1 cut(s) 112
ErhI CCWWGG 3 cut(s) 1590, 1776, 1983
FaqI GGGAC 2 cut(s) 1295, 3574
FauI CCCGC 1 cut(s) 852
FbaI TGATCA 2 cut(s) 3358, 3904
FblI GTMKAC 2 cut(s) 773, 3535
Fnu4HI GCNGC 8 cut(s) 264, 1587, 2154, 2187, 2670, 3497, 3765, 3768
FokI GGATG 4 cut(s) 2315, 3731, 3752, 3914
Fsp4HI GCNGC 8 cut(s) 264, 1587, 2154, 2187, 2670, 3497, 3765, 3768
FspBI CTAG 4 cut(s) 1223, 2462, 3015, 3710
GlaI GCGC 6 cut(s) 112, 951, 1370, 3423, 3443, 3866
GluI GCNGC 8 cut(s) 264, 1587, 2154, 2187, 2670, 3497, 3765, 3768
GsaI CCCAGC 1 cut(s) 3561
GsuI CTGGAG 4 cut(s) 393, 827, 998, 1710
HaeII RGCGCY 2 cut(s) 114, 3868
HapII CCGG 8 cut(s) 37, 50, 114, 156, 183, 216, 267, 3793
HgaI GACGC 2 cut(s) 334, 1004
HhaI GCGC 6 cut(s) 113, 952, 1371, 3424, 3444, 3867
Hin1I GRCGYC 1 cut(s) 111
Hin6I GCGC 6 cut(s) 111, 950, 1369, 3422, 3442, 3865
HinP1I GCGC 6 cut(s) 111, 950, 1369, 3422, 3442, 3865
HincII GTYRAC 2 cut(s) 853, 2260
HindII GTYRAC 2 cut(s) 853, 2260
HindIII AAGCTT 2 cut(s) 668, 2756
HpaI GTTAAC 2 cut(s) 853, 2260
HpaII CCGG 8 cut(s) 37, 50, 114, 156, 183, 216, 267, 3793
HphI GGTGA 8 cut(s) 61, 230, 1183, 1837, 2960, 3275, 3611, 3899
Hpy166II GTNNAC 8 cut(s) 13, 199, 475, 774, 853, 2096, 2260, 3536
Hpy8I GTNNAC 8 cut(s) 13, 199, 475, 774, 853, 2096, 2260, 3536
Hpy99I CGWCG 1 cut(s) 101
HpyCH4III ACNGT 8 cut(s) 10, 630, 1567, 1623, 1951, 1969, 2626, 3575
HpyCH4IV ACGT 2 cut(s) 63, 2646
HpyF10VI GCNNNNNNNGC 9 cut(s) 108, 676, 1592, 2162, 2363, 2983, 3370, 3450, 3699
HpySE526I ACGT 2 cut(s) 63, 2646
Hsp92I GRCGYC 1 cut(s) 111
HspAI GCGC 6 cut(s) 111, 950, 1369, 3422, 3442, 3865
KasI GGCGCC 1 cut(s) 110
Kpn2I TCCGGA 1 cut(s) 36
KroI GCCGGC 2 cut(s) 113, 266
KroNI GCCGGC 2 cut(s) 115, 268
Ksp22I TGATCA 2 cut(s) 3358, 3904
KspAI GTTAAC 2 cut(s) 853, 2260
LguI GCTCTTC 1 cut(s) 1155
LmnI GCTCC 6 cut(s) 39, 158, 185, 252, 2530, 2713
Lsp1109I GCAGC 6 cut(s) 1573, 2140, 2173, 2681, 3508, 3754
LweI GCATC 5 cut(s) 9, 714, 1046, 1285, 1885
MaeI CTAG 4 cut(s) 1223, 2462, 3015, 3710
MaeII ACGT 2 cut(s) 63, 2646
MaeIII GTNAC 4 cut(s) 1771, 3107, 3156, 3575
MbiI CCGCTC 1 cut(s) 859
MfeI CAATTG 2 cut(s) 490, 2222
MflI RGATCY 4 cut(s) 505, 1219, 2507, 3936
MhlI GDGCHC 5 cut(s) 477, 1091, 1150, 1799, 2624
MlsI TGGCCA 1 cut(s) 3861
MluNI TGGCCA 1 cut(s) 3861
Mly113I GGCGCC 1 cut(s) 111
MlyI GAGTC 9 cut(s) 1886, 1922, 2201, 2201, 2381, 2534, 2777, 3164, 3556
MmeI TCCRAC 6 cut(s) 612, 714, 1197, 2508, 2691, 3167
Mox20I TGGCCA 1 cut(s) 3861
Mph1103I ATGCAT 1 cut(s) 1464
MroI TCCGGA 1 cut(s) 36
MroNI GCCGGC 2 cut(s) 113, 266
MscI TGGCCA 1 cut(s) 3861
MslI CAYNNNNRTG 4 cut(s) 480, 1042, 1440, 3458
Msp20I TGGCCA 1 cut(s) 3861
MspA1I CMGCKG 2 cut(s) 3767, 3817
MspI CCGG 8 cut(s) 37, 50, 114, 156, 183, 216, 267, 3793
MspR9I CCNGG 5 cut(s) 50, 848, 3404, 3803, 3932
MunI CAATTG 2 cut(s) 490, 2222
Mva1269I GAATGC 2 cut(s) 2155, 3754
MvaI CCWGG 4 cut(s) 848, 3404, 3803, 3932
MvnI CGCG 2 cut(s) 675, 2844
MwoI GCNNNNNNNGC 9 cut(s) 108, 676, 1592, 2162, 2363, 2983, 3370, 3450, 3699
NaeI GCCGGC 2 cut(s) 115, 268
NarI GGCGCC 1 cut(s) 111
NciI CCSGG 1 cut(s) 50
NcoI CCATGG 2 cut(s) 1590, 1776
NgoMIV GCCGGC 2 cut(s) 113, 266
NlaIV GGNNCC 4 cut(s) 103, 112, 507, 2409
NmuCI GTSAC 1 cut(s) 3156
NruI TCGCGA 2 cut(s) 675, 2844
NsiI ATGCAT 1 cut(s) 1464
NspI RCATGY 1 cut(s) 3875
NspV TTCGAA 1 cut(s) 2915
PaeI GCATGC 1 cut(s) 3875
PagI TCATGA 1 cut(s) 3226
PceI AGGCCT 1 cut(s) 1928
PciSI GCTCTTC 1 cut(s) 1155
PctI GAATGC 2 cut(s) 2155, 3754
PdiI GCCGGC 2 cut(s) 115, 268
PflFI GACNNNGTC 1 cut(s) 344
PkrI GCNGC 8 cut(s) 265, 1588, 2155, 2188, 2671, 3498, 3766, 3769
PleI GAGTC 9 cut(s) 1886, 1921, 2200, 2201, 2381, 2533, 2776, 3163, 3556
PluTI GGCGCC 1 cut(s) 114
PpsI GAGTC 9 cut(s) 1886, 1921, 2200, 2201, 2381, 2533, 2776, 3163, 3556
PpuMI RGGWCCY 2 cut(s) 3400, 3646
PshBI ATTAAT 4 cut(s) 360, 1677, 2910, 3437
PsiI TTATAA 1 cut(s) 3482
Psp5II RGGWCCY 2 cut(s) 3400, 3646
Psp6I CCWGG 4 cut(s) 846, 3402, 3801, 3930
PspFI CCCAGC 1 cut(s) 3557
PspGI CCWGG 4 cut(s) 846, 3402, 3801, 3930
PspN4I GGNNCC 4 cut(s) 103, 112, 507, 2409
PspPPI RGGWCCY 2 cut(s) 3400, 3646
PstI CTGCAG 1 cut(s) 2191
PstNI CAGNNNCTG 2 cut(s) 2401, 3548
PsuI RGATCY 4 cut(s) 505, 1219, 2507, 3936
PsyI GACNNNGTC 1 cut(s) 344
PvuII CAGCTG 1 cut(s) 3817
RruI TCGCGA 2 cut(s) 675, 2844
RsaI GTAC 2 cut(s) 7, 3353
RsaNI GTAC 2 cut(s) 6, 3352
RseI CAYNNNNRTG 4 cut(s) 480, 1042, 1440, 3458
SapI GCTCTTC 1 cut(s) 1155
SatI GCNGC 8 cut(s) 264, 1587, 2154, 2187, 2670, 3497, 3765, 3768
SchI GAGTC 9 cut(s) 1886, 1922, 2201, 2201, 2381, 2534, 2777, 3164, 3556
ScrFI CCNGG 5 cut(s) 50, 848, 3404, 3803, 3932
SduI GDGCHC 5 cut(s) 477, 1091, 1150, 1799, 2624
SfaNI GCATC 5 cut(s) 9, 714, 1046, 1285, 1885
SfcI CTRYAG 3 cut(s) 626, 1965, 2187
SfoI GGCGCC 1 cut(s) 112
SfuI TTCGAA 1 cut(s) 2915
SgrAI CRCCGGYG 1 cut(s) 215
SinI GGWCC 6 cut(s) 258, 811, 2602, 3400, 3646, 3790
SmiMI CAYNNNNRTG 4 cut(s) 480, 1042, 1440, 3458
SphI GCATGC 1 cut(s) 3875
SseBI AGGCCT 1 cut(s) 1928
SsiI CCGC 8 cut(s) 250, 261, 264, 859, 1814, 3349, 3702, 3765
SspDI GGCGCC 1 cut(s) 110
SspI AATATT 3 cut(s) 709, 3091, 3884
SspMI CTAG 4 cut(s) 1223, 2462, 3015, 3710
StuI AGGCCT 1 cut(s) 1928
StyD4I CCNGG 5 cut(s) 48, 846, 3402, 3801, 3930
StyI CCWWGG 3 cut(s) 1590, 1776, 1983
TaaI ACNGT 8 cut(s) 10, 630, 1567, 1623, 1951, 1969, 2626, 3575
TaiI ACGT 2 cut(s) 66, 2649
TaqII GACCGA 1 cut(s) 2738
TauI GCSGC 2 cut(s) 266, 3767
TscAI CASTG 5 cut(s) 376, 907, 1188, 3165, 3720
TseFI GTSAC 1 cut(s) 3156
TseI GCWGC 6 cut(s) 1586, 2153, 2186, 2669, 3496, 3767
Tsp45I GTSAC 1 cut(s) 3156
TspRI CASTG 5 cut(s) 376, 907, 1188, 3165, 3720
Tth111I GACNNNGTC 1 cut(s) 344
VneI GTGCAC 1 cut(s) 473
VpaK11BI GGWCC 6 cut(s) 258, 811, 2602, 3400, 3646, 3790
VspI ATTAAT 4 cut(s) 360, 1677, 2910, 3437
XapI RAATTY 5 cut(s) 681, 765, 1505, 2126, 3294
XbaI TCTAGA 1 cut(s) 1222
XceI RCATGY 1 cut(s) 3875
XmiI GTMKAC 2 cut(s) 773, 3535
XspI CTAG 4 cut(s) 1223, 2462, 3015, 3710
Zsp2I ATGCAT 1 cut(s) 1464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.