Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0013193

Physical Location & Seq

Genomic Coordinates Forward (+)

18218847 .. 18224577

Gene Structure 5731 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g14550.t3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1383 bp

ATGTTAGCCGTCCGATCCAGGAAACCCGTCGGATACGACGACGTCCCGACTCTCCCCCTCTACCGCTCCGCCCCGGCTCTCGATGTCCGCCTCGAAGAGTTCGAGCTTTTCGCCATCGATCGCCTCCGAGTGCTTAAAGGGATATCTGACGGATTATCGAGAGGAAAGAAACATGATGAAATGGAGAAATTGATAAATGATCTTTGGAAAGCAAACATGAGGCATCCTCAGGCATCCGAGGCCATCAACAAGGACATTATATCGCACTTTGTTCTGCGTCTTGTTTATTGCAGAACGGACGAACTGAGGAAATGGTTTCTTTCCATGGAGACTGCCCTATTTCGCCACCGTTTTCGCAATGAAAGGCCTGAAGCTCAGAGGTCACTCATGGCAGAATTTGGTCTTGCCTACAAGAAAGTTGATAGTTCAGAACTCGAGAGTGTAATGGACAAACTGGCCCAGGTTGCGCGTTCCACGAGTCAATCTTTACCCACTGTTGATGCCATATTTTACAAGGTACCATTTGAAGAAGTTCCCGAACTTGTCGCTAGCCGTAGAGTATACATCAGCAAGGGGAATGCATATATAGCCATGAGTCAGGTGGTCTCCCTTGTTGCCACACATTTCCGCAGTCATCTGTCAAAGGCATTGATTTTGACTAACAGAAAGTGGACATCAACTATCGTAGAACAAGAGAAAAATCGGTTGACTCCTATAGTTGAAGCCCTCTCAACAAGCTACCTGGGCCCGGACTATTCTCAGCCAAAAGAATTTGCTGAGATATCATTAAGAGACATTGATGACATAGCTAAGAGCTCGTTTCCTATGTGCATGCGCCATCTATTTGAAAAGCTGAGAGAGGACCATCACCTAAAGCATGGAGGGAGAATGCAACTGGGTCTCTTTCTCAAGGGTGTTGGGTTAAAGCTGGATGATGCACTTGCATTTTGGAGAGCTGAGTTCTCACGAAAGGTTGGTGCCGAGAGGTTTGACAAAGAATATGCATATAGCATACGCCACAATTATGGAAGAGAAGGAAAAAGAACGGATTACACGCCTTATTCCTGTCAAAAGATCATCTCATCTACGCCTGGCGTAGGAGATCATCATGGATGCCCATATCGACATTTCAGTGAAGAGAATCTGACAGCTGCTCTCAGTAGAATGGGAGTTAACAGTCGTGCTCTTAGTGATGTAATGAACAAAGTTCGGAATAAGCATTATCAGTTGGCATGCACCTTAACGTTTGAAGCTGTCCATGGGACATCATGCGAAGCTGGGATTAACCATCCAAATCAATACTTCTCTGACAGTCAAATAATTATGAAGTCAAAGAATCAATCTACAGCGAAGGACACGCAAATGGAAATAGAATCTTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000428 GO:0003674 GO:0003676 GO:0003677 GO:0003697 GO:0003824 GO:0003887 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005658 GO:0005694 GO:0006139 GO:0006259 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030880 GO:0030894 GO:0031974 GO:0031981 GO:0032991 GO:0032993 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0043601 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0055029 GO:0061695 GO:0070013 GO:0071704 GO:0071897 GO:0090304 GO:0097159 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03030 map00230 map00240 map01100 map03030

Pfam Domains

Protein Families

DNA_primase_lrg

Protein Analysis

461

Amino Acids

52.68

Weight (kDa)

9.05

Isoelectric Point (pI)

42.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_primase_lrg_N	PF26466	24 - 225	9.9e-58	Eukaryotic and archaeal DNA primase, large subunit N-terminal domain
DNA_primase_lrg	PF04104	268 - 376	6e-45	Eukaryotic and archaeal DNA primase, large subunit C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0013193)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67320 AT1G67320
fragaria_vesca	FvH4_4g14550 FvH4_4g14550 FvH4_4g14550
malus_domestica	MD09G1083700.v1.1 MD13G1121900.v1.1 MD16G1122200.v1.1
prunus_persica	Prupe.1G223400_v2.0.a1
pyrus_communis	pycom13g10610
rosa_chinensis	RchiOBHm_Chr4g0418271
rosa_laevigata	RLG00000007881
rosa_multiflora	Rmu_sc0000032.1_g000005
rosa_roxburghii	Rroxscaffold_5G00361450
rosa_rugosa	Rorug04G0151600
rosa_samantha	Rh4AG213200 Rh4CG224900
rosa_wichuraiana	Rw4G018110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	45
Acc65I	GGTACC	1 cut(s)	517
AccB1I	GGYRCC	2 cut(s)	517, 977
AccBSI	CCGCTC	1 cut(s)	66
AccI	GTMKAC	1 cut(s)	561
AccII	CGCG	1 cut(s)	469
AciI	CCGC	4 cut(s)	64, 69, 88, 628
AclI	AACGTT	1 cut(s)	1244
AclWI	GGATC	1 cut(s)	9
AcsI	RAATTY	2 cut(s)	395, 770
AcuI	CTGAAG	1 cut(s)	390
AcyI	GRCGYC	1 cut(s)	42
AfaI	GTAC	1 cut(s)	519
AfiI	CCNNNNNNNGG	2 cut(s)	748, 1097
AgsI	TTSAA	4 cut(s)	527, 722, 848, 1250
AjnI	CCWGG	4 cut(s)	17, 459, 741, 1090
Alw21I	GWGCWC	2 cut(s)	818, 1186
Alw26I	GTCTC	4 cut(s)	323, 610, 786, 905
AlwI	GGATC	1 cut(s)	9
Ama87I	CYCGRG	1 cut(s)	434
AoxI	GGCC	4 cut(s)	240, 365, 456, 745
ApaI	GGGCCC	1 cut(s)	749
ApeKI	GCWGC	1 cut(s)	1151
ApoI	RAATTY	2 cut(s)	395, 770
Asp700I	GAANNNNTTC	1 cut(s)	531
Asp718I	GGTACC	1 cut(s)	517
AspLEI	GCGC	2 cut(s)	469, 837
AspS9I	GGNCC	4 cut(s)	457, 745, 746, 862
AsuC2I	CCSGG	2 cut(s)	74, 749
AsuHPI	GGTGA	1 cut(s)	860
AsuNHI	GCTAGC	1 cut(s)	548
AvaI	CYCGRG	1 cut(s)	434
AvaII	GGWCC	1 cut(s)	862
AxyI	CCTNAGG	1 cut(s)	228
BaeGI	GKGCMC	1 cut(s)	749
BanI	GGYRCC	2 cut(s)	517, 977
BanII	GRGCYC	2 cut(s)	749, 818
BauI	CACGAG	1 cut(s)	475
Bbv12I	GWGCWC	2 cut(s)	818, 1186
BbvI	GCAGC	1 cut(s)	1138
BccI	CCATC	5 cut(s)	122, 251, 846, 873, 1296
BceAI	ACGGC	1 cut(s)	537
BciT130I	CCWGG	4 cut(s)	19, 461, 743, 1092
BciVI	GTATCC	1 cut(s)	26
BcnI	CCSGG	2 cut(s)	74, 749
BcoDI	GTCTC	4 cut(s)	323, 610, 786, 905
BfaI	CTAG	1 cut(s)	549
BfmI	CTRYAG	2 cut(s)	714, 1344
BfuI	GTATCC	1 cut(s)	26
BisI	GCNGC	1 cut(s)	1152
BlsI	GCNGC	1 cut(s)	1153
Bme1390I	CCNGG	6 cut(s)	19, 74, 461, 743, 749, 1092
Bme18I	GGWCC	1 cut(s)	862
BmeT110I	CYCGRG	1 cut(s)	434
BmgT120I	GGNCC	4 cut(s)	457, 745, 746, 862
BmiI	GGNNCC	3 cut(s)	519, 747, 979
BmrFI	CCNGG	6 cut(s)	19, 74, 461, 743, 749, 1092
BmrI	ACTGGG	1 cut(s)	905
BmsI	GCATC	5 cut(s)	232, 242, 490, 925, 1103
BmtI	GCTAGC	1 cut(s)	552
BmuI	ACTGGG	1 cut(s)	905
BplI	GAGNNNNNCTC	2 cut(s)	211, 243
BpuEI	CTTGAG	1 cut(s)	893
BpuMI	CCSGG	2 cut(s)	74, 749
Bsa29I	ATCGAT	1 cut(s)	117
BsaHI	GRCGYC	1 cut(s)	42
BsaI	GGTCTC	2 cut(s)	610, 905
BsaJI	CCNNGG	6 cut(s)	72, 237, 324, 459, 742, 1258
Bsc4I	CCNNNNNNNGG	2 cut(s)	748, 1097
Bse1I	ACTGG	2 cut(s)	459, 900
Bse21I	CCTNAGG	1 cut(s)	228
Bse3DI	GCAATG	1 cut(s)	364
BseBI	CCWGG	4 cut(s)	19, 461, 743, 1092
BseCI	ATCGAT	1 cut(s)	117
BseDI	CCNNGG	6 cut(s)	72, 237, 324, 459, 742, 1258
BseGI	GGATG	5 cut(s)	223, 233, 937, 1118, 1288
BseLI	CCNNNNNNNGG	2 cut(s)	748, 1097
BseMI	GCAATG	1 cut(s)	364
BseMII	CTCAG	8 cut(s)	242, 296, 389, 768, 773, 845, 948, 1171
BseNI	ACTGG	2 cut(s)	459, 900
BseSI	GKGCMC	1 cut(s)	749
BseXI	GCAGC	1 cut(s)	1138
BseYI	CCCAGC	1 cut(s)	1277
Bsh1236I	CGCG	1 cut(s)	469
Bsh1285I	CGRYCG	1 cut(s)	121
BshFI	GGCC	4 cut(s)	242, 367, 458, 747
BshNI	GGYRCC	2 cut(s)	517, 977
BshVI	ATCGAT	1 cut(s)	117
BsiEI	CGRYCG	1 cut(s)	121
BsiHKAI	GWGCWC	2 cut(s)	818, 1186
BsiHKCI	CYCGRG	1 cut(s)	434
BsiSI	CCGG	2 cut(s)	74, 749
BslFI	GGGAC	2 cut(s)	29, 1276
BslI	CCNNNNNNNGG	2 cut(s)	748, 1097
BsmAI	GTCTC	4 cut(s)	323, 610, 786, 905
BsmFI	GGGAC	2 cut(s)	29, 1276
BsmI	GAATGC	2 cut(s)	583, 894
BsnI	GGCC	4 cut(s)	242, 367, 458, 747
Bso31I	GGTCTC	2 cut(s)	610, 905
BsoBI	CYCGRG	1 cut(s)	434
Bsp120I	GGGCCC	1 cut(s)	745
Bsp1286I	GDGCHC	3 cut(s)	749, 818, 1186
Bsp143I	GATC	5 cut(s)	14, 118, 199, 1074, 1102
Bsp19I	CCATGG	2 cut(s)	324, 1258
BspACI	CCGC	4 cut(s)	64, 69, 88, 628
BspANI	GGCC	4 cut(s)	242, 367, 458, 747
BspCNI	CTCAG	8 cut(s)	241, 297, 388, 769, 772, 846, 949, 1170
BspDI	ATCGAT	1 cut(s)	117
BspFNI	CGCG	1 cut(s)	469
BspLI	GGNNCC	3 cut(s)	519, 747, 979
BspOI	GCTAGC	1 cut(s)	552
BspPI	GGATC	1 cut(s)	9
BspT107I	GGYRCC	2 cut(s)	517, 977
BspTNI	GGTCTC	2 cut(s)	610, 905
BsrBI	CCGCTC	1 cut(s)	66
BsrDI	GCAATG	1 cut(s)	364
BsrI	ACTGG	2 cut(s)	459, 900
BssECI	CCNNGG	6 cut(s)	72, 237, 324, 459, 742, 1258
BssMI	GATC	5 cut(s)	14, 118, 199, 1074, 1102
BssNAI	GTATAC	1 cut(s)	562
BssNI	GRCGYC	1 cut(s)	42
BssSI	CACGAG	1 cut(s)	475
BssT1I	CCWWGG	2 cut(s)	324, 1258
Bst1107I	GTATAC	1 cut(s)	562
Bst2BI	CACGAG	1 cut(s)	475
Bst2UI	CCWGG	4 cut(s)	19, 461, 743, 1092
Bst4CI	ACNGT	4 cut(s)	350, 496, 1178, 1313
Bst6I	CTCTTC	3 cut(s)	90, 1024, 1131
BstACI	GRCGYC	1 cut(s)	42
BstC8I	GCNNGC	3 cut(s)	550, 833, 1234
BstDSI	CCRYGG	2 cut(s)	324, 1258
BstENI	CCTNNNNNAGG	1 cut(s)	1095
BstF5I	GGATG	5 cut(s)	223, 233, 937, 1118, 1288
BstFNI	CGCG	1 cut(s)	469
BstHHI	GCGC	2 cut(s)	469, 837
BstKTI	GATC	5 cut(s)	17, 121, 202, 1077, 1105
BstMAI	GTCTC	4 cut(s)	323, 610, 786, 905
BstMBI	GATC	5 cut(s)	14, 118, 199, 1074, 1102
BstMCI	CGRYCG	1 cut(s)	121
BstMWI	GCNNNNNNNGC	4 cut(s)	239, 464, 587, 744
BstNI	CCWGG	4 cut(s)	19, 461, 743, 1092
BstNSI	RCATGY	2 cut(s)	835, 1236
BstSCI	CCNGG	6 cut(s)	17, 72, 459, 741, 747, 1090
BstSFI	CTRYAG	2 cut(s)	714, 1344
BstSLI	GKGCMC	1 cut(s)	749
BstUI	CGCG	1 cut(s)	469
BstV1I	GCAGC	1 cut(s)	1138
BstXI	CCANNNNNNTGG	1 cut(s)	1025
BstZ17I	GTATAC	1 cut(s)	562
Bsu15I	ATCGAT	1 cut(s)	117
Bsu36I	CCTNAGG	1 cut(s)	228
BsuI	GTATCC	1 cut(s)	26
BsuRI	GGCC	4 cut(s)	242, 367, 458, 747
BsuTUI	ATCGAT	1 cut(s)	117
BtgI	CCRYGG	2 cut(s)	324, 1258
BtsCI	GGATG	5 cut(s)	223, 233, 937, 1118, 1288
BtsIMutI	CAGTG	2 cut(s)	492, 1138
Cac8I	GCNNGC	3 cut(s)	550, 833, 1234
CfoI	GCGC	2 cut(s)	469, 837
Cfr13I	GGNCC	4 cut(s)	457, 745, 746, 862
ClaI	ATCGAT	1 cut(s)	117
CseI	GACGC	1 cut(s)	266
Csp6I	GTAC	1 cut(s)	518
CviQI	GTAC	1 cut(s)	518
DpnI	GATC	5 cut(s)	16, 120, 201, 1076, 1104
DpnII	GATC	5 cut(s)	14, 118, 199, 1074, 1102
Eam1104I	CTCTTC	3 cut(s)	90, 1024, 1131
EarI	CTCTTC	3 cut(s)	90, 1024, 1131
EciI	GGCGGA	2 cut(s)	58, 77
Ecl136II	GAGCTC	1 cut(s)	816
Eco130I	CCWWGG	2 cut(s)	324, 1258
Eco147I	AGGCCT	1 cut(s)	367
Eco24I	GRGCYC	2 cut(s)	749, 818
Eco31I	GGTCTC	2 cut(s)	610, 905
Eco32I	GATATC	2 cut(s)	144, 783
Eco47I	GGWCC	1 cut(s)	862
Eco53kI	GAGCTC	1 cut(s)	816
Eco57I	CTGAAG	1 cut(s)	390
Eco81I	CCTNAGG	1 cut(s)	228
Eco88I	CYCGRG	1 cut(s)	434
EcoICRI	GAGCTC	1 cut(s)	816
EcoNI	CCTNNNNNAGG	1 cut(s)	1095
EcoRII	CCWGG	4 cut(s)	17, 459, 741, 1090
EcoRV	GATATC	2 cut(s)	144, 783
EcoT14I	CCWWGG	2 cut(s)	324, 1258
EcoT22I	ATGCAT	2 cut(s)	583, 1006
EcoT38I	GRGCYC	2 cut(s)	749, 818
ErhI	CCWWGG	2 cut(s)	324, 1258
FaqI	GGGAC	2 cut(s)	29, 1276
FblI	GTMKAC	1 cut(s)	561
Fnu4HI	GCNGC	1 cut(s)	1152
FokI	GGATG	5 cut(s)	210, 220, 944, 1125, 1275
FriOI	GRGCYC	2 cut(s)	749, 818
Fsp4HI	GCNGC	1 cut(s)	1152
FspBI	CTAG	1 cut(s)	549
GlaI	GCGC	2 cut(s)	468, 836
GluI	GCNGC	1 cut(s)	1152
GsaI	CCCAGC	1 cut(s)	1281
HaeIII	GGCC	4 cut(s)	242, 367, 458, 747
HapII	CCGG	2 cut(s)	74, 749
HgaI	GACGC	1 cut(s)	266
HhaI	GCGC	2 cut(s)	469, 837
Hin1I	GRCGYC	1 cut(s)	42
Hin6I	GCGC	2 cut(s)	467, 835
HinP1I	GCGC	2 cut(s)	467, 835
HincII	GTYRAC	2 cut(s)	708, 1174
HindII	GTYRAC	2 cut(s)	708, 1174
HinfI	GANTC	7 cut(s)	49, 478, 595, 709, 1141, 1336, 1373
HpaI	GTTAAC	1 cut(s)	1174
HpaII	CCGG	2 cut(s)	74, 749
HphI	GGTGA	1 cut(s)	860
Hpy166II	GTNNAC	4 cut(s)	562, 672, 708, 1174
Hpy188III	TCNNGA	7 cut(s)	46, 80, 159, 436, 536, 966, 1380
Hpy8I	GTNNAC	4 cut(s)	562, 672, 708, 1174
Hpy99I	CGWCG	3 cut(s)	32, 41, 44
HpyAV	CCTTC	2 cut(s)	1028, 1345
HpyCH4III	ACNGT	4 cut(s)	350, 496, 1178, 1313
HpyCH4IV	ACGT	2 cut(s)	42, 1244
HpyCH4V	TGCA	8 cut(s)	291, 581, 831, 892, 938, 944, 1004, 1236
HpyF10VI	GCNNNNNNNGC	4 cut(s)	239, 464, 587, 744
HpySE526I	ACGT	2 cut(s)	42, 1244
Hsp92I	GRCGYC	1 cut(s)	42
HspAI	GCGC	2 cut(s)	467, 835
KpnI	GGTACC	1 cut(s)	521
KspAI	GTTAAC	1 cut(s)	1174
Kzo9I	GATC	5 cut(s)	14, 118, 199, 1074, 1102
LmnI	GCTCC	1 cut(s)	71
Lsp1109I	GCAGC	1 cut(s)	1138
LweI	GCATC	5 cut(s)	232, 242, 490, 925, 1103
MaeI	CTAG	1 cut(s)	549
MaeII	ACGT	2 cut(s)	42, 1244
MaeIII	GTNAC	1 cut(s)	381
MalI	GATC	5 cut(s)	16, 120, 201, 1076, 1104
MbiI	CCGCTC	1 cut(s)	66
MboI	GATC	5 cut(s)	14, 118, 199, 1074, 1102
MboII	GAAGA	5 cut(s)	107, 539, 1041, 1148, 1368
MhlI	GDGCHC	3 cut(s)	749, 818, 1186
MluCI	AATT	5 cut(s)	188, 395, 770, 1021, 1320
MlyI	GAGTC	4 cut(s)	43, 487, 604, 703
MmeI	TCCRAC	1 cut(s)	10
Mph1103I	ATGCAT	2 cut(s)	583, 1006
MroXI	GAANNNNTTC	1 cut(s)	531
MseI	TTAA	6 cut(s)	135, 788, 923, 1173, 1241, 1284
MslI	CAYNNNNRTG	3 cut(s)	1023, 1131, 1361
MspA1I	CMGCKG	1 cut(s)	1151
MspI	CCGG	2 cut(s)	74, 749
MspR9I	CCNGG	6 cut(s)	19, 74, 461, 743, 749, 1092
Mva1269I	GAATGC	2 cut(s)	583, 894
MvaI	CCWGG	4 cut(s)	19, 461, 743, 1092
MvnI	CGCG	1 cut(s)	469
MwoI	GCNNNNNNNGC	4 cut(s)	239, 464, 587, 744
NciI	CCSGG	2 cut(s)	74, 749
NcoI	CCATGG	2 cut(s)	324, 1258
NdeII	GATC	5 cut(s)	14, 118, 199, 1074, 1102
NheI	GCTAGC	1 cut(s)	548
NlaIV	GGNNCC	3 cut(s)	519, 747, 979
NmeAIII	GCCGAG	1 cut(s)	1006
NmuCI	GTSAC	1 cut(s)	381
NsiI	ATGCAT	2 cut(s)	583, 1006
NspI	RCATGY	2 cut(s)	835, 1236
PaeI	GCATGC	2 cut(s)	835, 1236
PaeR7I	CTCGAG	1 cut(s)	434
PceI	AGGCCT	1 cut(s)	367
PcsI	WCGNNNNNNNCGW	2 cut(s)	36, 99
PctI	GAATGC	2 cut(s)	583, 894
PdmI	GAANNNNTTC	1 cut(s)	531
PfeI	GAWTC	3 cut(s)	1141, 1336, 1373
PflFI	GACNNNGTC	1 cut(s)	41
PfoI	TCCNGGA	1 cut(s)	17
PkrI	GCNGC	1 cut(s)	1153
Ple19I	CGATCG	1 cut(s)	121
PleI	GAGTC	4 cut(s)	43, 486, 603, 703
PpsI	GAGTC	4 cut(s)	43, 486, 603, 703
Psp124BI	GAGCTC	1 cut(s)	818
Psp1406I	AACGTT	1 cut(s)	1244
Psp6I	CCWGG	4 cut(s)	17, 459, 741, 1090
PspFI	CCCAGC	1 cut(s)	1277
PspGI	CCWGG	4 cut(s)	17, 459, 741, 1090
PspN4I	GGNNCC	3 cut(s)	519, 747, 979
PspOMI	GGGCCC	1 cut(s)	745
PspPI	GGNCC	4 cut(s)	457, 745, 746, 862
PsyI	GACNNNGTC	1 cut(s)	41
PvuI	CGATCG	1 cut(s)	121
PvuII	CAGCTG	1 cut(s)	1151
RsaI	GTAC	1 cut(s)	519
RsaNI	GTAC	1 cut(s)	518
RseI	CAYNNNNRTG	3 cut(s)	1023, 1131, 1361
SacI	GAGCTC	1 cut(s)	818
SaqAI	TTAA	6 cut(s)	135, 788, 923, 1173, 1241, 1284
SatI	GCNGC	1 cut(s)	1152
Sau3AI	GATC	5 cut(s)	14, 118, 199, 1074, 1102
Sau96I	GGNCC	4 cut(s)	457, 745, 746, 862
SchI	GAGTC	4 cut(s)	43, 487, 604, 703
ScrFI	CCNGG	6 cut(s)	19, 74, 461, 743, 749, 1092
SduI	GDGCHC	3 cut(s)	749, 818, 1186
SfaNI	GCATC	5 cut(s)	232, 242, 490, 925, 1103
SfcI	CTRYAG	2 cut(s)	714, 1344
Sfr274I	CTCGAG	1 cut(s)	434
SinI	GGWCC	1 cut(s)	862
SlaI	CTCGAG	1 cut(s)	434
SmiMI	CAYNNNNRTG	3 cut(s)	1023, 1131, 1361
SmlI	CTYRAG	2 cut(s)	434, 908
SmoI	CTYRAG	2 cut(s)	434, 908
SphI	GCATGC	2 cut(s)	835, 1236
Sse9I	AATT	5 cut(s)	188, 395, 770, 1021, 1320
SseBI	AGGCCT	1 cut(s)	367
SsiI	CCGC	4 cut(s)	64, 69, 88, 628
SspMI	CTAG	1 cut(s)	549
SstI	GAGCTC	1 cut(s)	818
StuI	AGGCCT	1 cut(s)	367
StyD4I	CCNGG	6 cut(s)	17, 72, 459, 741, 747, 1090
StyI	CCWWGG	2 cut(s)	324, 1258
TaaI	ACNGT	4 cut(s)	350, 496, 1178, 1313
TaiI	ACGT	2 cut(s)	45, 1247
TaqI	TCGA	7 cut(s)	81, 93, 102, 117, 158, 435, 1123
TasI	AATT	5 cut(s)	188, 395, 770, 1021, 1320
TfiI	GAWTC	3 cut(s)	1141, 1336, 1373
Tru1I	TTAA	6 cut(s)	135, 788, 923, 1173, 1241, 1284
Tru9I	TTAA	6 cut(s)	135, 788, 923, 1173, 1241, 1284
TscAI	CASTG	2 cut(s)	499, 1138
TseFI	GTSAC	1 cut(s)	381
TseI	GCWGC	1 cut(s)	1151
Tsp45I	GTSAC	1 cut(s)	381
TspDTI	ATGAA	4 cut(s)	192, 375, 1214, 1340
TspGWI	ACGGA	3 cut(s)	165, 311, 1061
TspRI	CASTG	2 cut(s)	499, 1138
Tth111I	GACNNNGTC	1 cut(s)	41
VpaK11BI	GGWCC	1 cut(s)	862
XagI	CCTNNNNNAGG	1 cut(s)	1095
XapI	RAATTY	2 cut(s)	395, 770
XceI	RCATGY	2 cut(s)	835, 1236
XcmI	CCANNNNNNNNNTGG	1 cut(s)	598
XhoI	CTCGAG	1 cut(s)	434
XmiI	GTMKAC	1 cut(s)	561
XmnI	GAANNNNTTC	1 cut(s)	531
XspI	CTAG	1 cut(s)	549
ZraI	GACGTC	1 cut(s)	43
Zsp2I	ATGCAT	2 cut(s)	583, 1006

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.