Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0013193

Physical Location & Seq

Genomic Coordinates Reverse (-)

8797241 .. 8800514

Gene Structure 3274 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1122200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 660 bp

ATGAGAAATGCGAGCAATCTTTGCATTTGGACTCTCACGTTTGCACATGTAGTGGCCCACCGGAGAGAGAGGAGCAAGATTGCAAGCCGTTCGATCTCAGAGGAAATCTTTCATCTCCCGACCCTCCCTCTTTACCGCTCCGCTCCCGCCCTCGAAGTTCGTCTCGAAGAGTTCGAGCTCTTCGCCCTCGATCGCCTCCGAGTGGTTACAGGGATTTCTGATGGATTATCACGCGGAAAGAAACCTGACGAAATGCAGAAGTTGGCAATAGAACTATGGAAGACAAATATGAGGCATCCTCAGGCATCTGAGGTCGTCAACAAGGACATAATATCGCACTTTGTTCTGCGACTAGTTTATTGCAGAACGGAGGACCTGAGAAAATGGTTTCTTTCCATGGAGACTGCCTTATTTCGTTACCGATTTATGAATGAACAACCTGAACCGCAGCGGGCACTCATGGAAGAATTTGATCTTCCATACAAGGCAGTCAGTGGTTCAGAACTTGAGGGTGTTGGGTTGAAGCTGGATGATGCCCTTGGGTTTTGGAAAGCTGAGTTCTCGCAAAAAGTTGGTGCTGAAAGGTTTGACAAAGAGTATTCGTATGGCATACGCCACAATTATGGAAGAGAGGGGAAAAGAACGGATTACACACCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000428 GO:0003674 GO:0003676 GO:0003677 GO:0003697 GO:0003824 GO:0003887 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005658 GO:0005694 GO:0006139 GO:0006259 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030880 GO:0030894 GO:0031974 GO:0031981 GO:0032991 GO:0032993 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0043601 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0055029 GO:0061695 GO:0070013 GO:0071704 GO:0071897 GO:0090304 GO:0097159 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03030 map00230 map00240 map01100 map03030

Pfam Domains

Protein Families

DNA_primase_lrg

Protein Analysis

220

Amino Acids

25.62

Weight (kDa)

8.47

Isoelectric Point (pI)

39.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_primase_lrg_N	PF26466	48 - 170	1.3e-30	Eukaryotic and archaeal DNA primase, large subunit N-terminal domain
DNA_primase_lrg	PF04104	169 - 219	7.2e-13	Eukaryotic and archaeal DNA primase, large subunit C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0013193)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67320 AT1G67320
fragaria_vesca	FvH4_4g14550 FvH4_4g14550 FvH4_4g14550
malus_domestica	MD09G1083700.v1.1 MD13G1121900.v1.1 MD16G1122200.v1.1
prunus_persica	Prupe.1G223400_v2.0.a1
pyrus_communis	pycom13g10610
rosa_chinensis	RchiOBHm_Chr4g0418271
rosa_laevigata	RLG00000007881
rosa_multiflora	Rmu_sc0000032.1_g000005
rosa_roxburghii	Rroxscaffold_5G00361450
rosa_rugosa	Rorug04G0151600
rosa_samantha	Rh4AG213200 Rh4CG224900
rosa_wichuraiana	Rw4G018110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	2 cut(s)	138, 143
AccII	CGCG	1 cut(s)	234
AciI	CCGC	6 cut(s)	136, 141, 147, 234, 446, 451
AcsI	RAATTY	1 cut(s)	467
AfiI	CCNNNNNNNGG	1 cut(s)	202
AflIII	ACRYGT	1 cut(s)	46
AgsI	TTSAA	1 cut(s)	523
AhlI	ACTAGT	1 cut(s)	352
AluBI	AGCT	3 cut(s)	178, 526, 554
AluI	AGCT	3 cut(s)	178, 526, 554
Alw21I	GWGCWC	1 cut(s)	180
Alw26I	GTCTC	2 cut(s)	167, 395
AoxI	GGCC	1 cut(s)	54
ApeKI	GCWGC	1 cut(s)	448
ApoI	RAATTY	1 cut(s)	467
Asp700I	GAANNNNTTC	1 cut(s)	108
AspS9I	GGNCC	2 cut(s)	55, 373
AvaII	GGWCC	1 cut(s)	373
AxyI	CCTNAGG	1 cut(s)	300
BaeGI	GKGCMC	1 cut(s)	457
BanII	GRGCYC	1 cut(s)	180
BbsI	GAAGAC	1 cut(s)	287
Bbv12I	GWGCWC	1 cut(s)	180
BbvI	GCAGC	1 cut(s)	460
BccI	CCATC	1 cut(s)	215
BceAI	ACGGC	1 cut(s)	72
BcgI	CGANNNNNNTGC	2 cut(s)	72, 106
BcoDI	GTCTC	2 cut(s)	167, 395
BcuI	ACTAGT	1 cut(s)	352
BfaI	CTAG	1 cut(s)	353
BisI	GCNGC	1 cut(s)	449
BlsI	GCNGC	1 cut(s)	450
Bme18I	GGWCC	1 cut(s)	373
BmgT120I	GGNCC	2 cut(s)	55, 373
BmsI	GCATC	3 cut(s)	304, 314, 523
BpiI	GAAGAC	1 cut(s)	287
BplI	GAGNNNNNCTC	2 cut(s)	283, 315
BpuEI	CTTGAG	1 cut(s)	527
BsaJI	CCNNGG	2 cut(s)	396, 538
BsaWI	WCCGGW	1 cut(s)	60
Bsc4I	CCNNNNNNNGG	1 cut(s)	202
Bse21I	CCTNAGG	1 cut(s)	300
BseDI	CCNNGG	2 cut(s)	396, 538
BseGI	GGATG	2 cut(s)	295, 535
BseLI	CCNNNNNNNGG	1 cut(s)	202
BseMII	CTCAG	5 cut(s)	111, 300, 314, 368, 546
BseRI	GAGGAG	1 cut(s)	85
BseSI	GKGCMC	1 cut(s)	457
BseXI	GCAGC	1 cut(s)	460
Bsh1236I	CGCG	1 cut(s)	234
Bsh1285I	CGRYCG	1 cut(s)	193
BshFI	GGCC	1 cut(s)	56
BsiEI	CGRYCG	1 cut(s)	193
BsiHKAI	GWGCWC	1 cut(s)	180
BsiSI	CCGG	1 cut(s)	61
BslI	CCNNNNNNNGG	1 cut(s)	202
BsmAI	GTCTC	2 cut(s)	167, 395
BsmBI	CGTCTC	1 cut(s)	167
BsnI	GGCC	1 cut(s)	56
Bsp1286I	GDGCHC	2 cut(s)	180, 457
Bsp143I	GATC	3 cut(s)	93, 190, 472
Bsp19I	CCATGG	1 cut(s)	396
BspACI	CCGC	6 cut(s)	136, 141, 147, 234, 446, 451
BspANI	GGCC	1 cut(s)	56
BspCNI	CTCAG	5 cut(s)	110, 301, 313, 369, 547
BspFNI	CGCG	1 cut(s)	234
BspQI	GCTCTTC	1 cut(s)	185
BsrBI	CCGCTC	2 cut(s)	138, 143
BssECI	CCNNGG	2 cut(s)	396, 538
BssMI	GATC	3 cut(s)	93, 190, 472
BssT1I	CCWWGG	2 cut(s)	396, 538
Bst6I	CTCTTC	3 cut(s)	162, 185, 622
BstAPI	GCANNNNNTGC	1 cut(s)	21
BstC8I	GCNNGC	3 cut(s)	13, 85, 453
BstDEI	CTNAG	5 cut(s)	97, 300, 309, 377, 555
BstDSI	CCRYGG	1 cut(s)	396
BstF5I	GGATG	2 cut(s)	295, 535
BstFNI	CGCG	1 cut(s)	234
BstKTI	GATC	3 cut(s)	96, 193, 475
BstMAI	GTCTC	2 cut(s)	167, 395
BstMBI	GATC	3 cut(s)	93, 190, 472
BstMCI	CGRYCG	1 cut(s)	193
BstMWI	GCNNNNNNNGC	1 cut(s)	21
BstNSI	RCATGY	1 cut(s)	50
BstSLI	GKGCMC	1 cut(s)	457
BstUI	CGCG	1 cut(s)	234
BstV1I	GCAGC	1 cut(s)	460
BstV2I	GAAGAC	1 cut(s)	287
BstXI	CCANNNNNNTGG	1 cut(s)	623
Bsu36I	CCTNAGG	1 cut(s)	300
BsuRI	GGCC	1 cut(s)	56
BtgI	CCRYGG	1 cut(s)	396
BtsCI	GGATG	2 cut(s)	295, 535
BtsIMutI	CAGTG	1 cut(s)	499
Cac8I	GCNNGC	3 cut(s)	13, 85, 453
Cfr13I	GGNCC	2 cut(s)	55, 373
CviAII	CATG	3 cut(s)	47, 397, 460
CviJI	RGCY	5 cut(s)	56, 87, 178, 526, 554
CviKI_1	RGCY	5 cut(s)	56, 87, 178, 526, 554
DdeI	CTNAG	5 cut(s)	97, 300, 309, 377, 555
DpnI	GATC	3 cut(s)	95, 192, 474
DpnII	GATC	3 cut(s)	93, 190, 472
Eam1104I	CTCTTC	3 cut(s)	162, 185, 622
EarI	CTCTTC	3 cut(s)	162, 185, 622
Ecl136II	GAGCTC	1 cut(s)	178
Eco130I	CCWWGG	2 cut(s)	396, 538
Eco24I	GRGCYC	1 cut(s)	180
Eco47I	GGWCC	1 cut(s)	373
Eco53kI	GAGCTC	1 cut(s)	178
Eco81I	CCTNAGG	1 cut(s)	300
EcoICRI	GAGCTC	1 cut(s)	178
EcoO109I	RGGNCCY	1 cut(s)	373
EcoT14I	CCWWGG	2 cut(s)	396, 538
EcoT38I	GRGCYC	1 cut(s)	180
ErhI	CCWWGG	2 cut(s)	396, 538
Esp3I	CGTCTC	1 cut(s)	167
FaeI	CATG	3 cut(s)	50, 400, 463
FatI	CATG	3 cut(s)	46, 396, 459
FauI	CCCGC	2 cut(s)	154, 444
Fnu4HI	GCNGC	1 cut(s)	449
FokI	GGATG	2 cut(s)	282, 542
FriOI	GRGCYC	1 cut(s)	180
Fsp4HI	GCNGC	1 cut(s)	449
FspBI	CTAG	1 cut(s)	353
GluI	GCNGC	1 cut(s)	449
HaeIII	GGCC	1 cut(s)	56
HapII	CCGG	1 cut(s)	61
Hin1II	CATG	3 cut(s)	50, 400, 463
HincII	GTYRAC	1 cut(s)	319
HindII	GTYRAC	1 cut(s)	319
HinfI	GANTC	1 cut(s)	31
HpaII	CCGG	1 cut(s)	61
Hpy166II	GTNNAC	1 cut(s)	319
Hpy188I	TCNGA	5 cut(s)	100, 200, 220, 310, 502
Hpy188III	TCNNGA	2 cut(s)	118, 164
Hpy8I	GTNNAC	1 cut(s)	319
HpyCH4IV	ACGT	1 cut(s)	38
HpyCH4V	TGCA	5 cut(s)	24, 44, 83, 256, 363
HpyF10VI	GCNNNNNNNGC	1 cut(s)	21
HpyF3I	CTNAG	5 cut(s)	97, 300, 309, 377, 555
HpySE526I	ACGT	1 cut(s)	38
Hsp92II	CATG	3 cut(s)	50, 400, 463
Kzo9I	GATC	3 cut(s)	93, 190, 472
LguI	GCTCTTC	1 cut(s)	185
LmnI	GCTCC	3 cut(s)	72, 143, 148
LpnPI	CCDG	7 cut(s)	74, 195, 258, 287, 389, 453, 512
Lsp1109I	GCAGC	1 cut(s)	460
LweI	GCATC	3 cut(s)	304, 314, 523
MaeI	CTAG	1 cut(s)	353
MaeII	ACGT	1 cut(s)	38
MaeIII	GTNAC	2 cut(s)	205, 416
MalI	GATC	3 cut(s)	95, 192, 474
MbiI	CCGCTC	2 cut(s)	138, 143
MboI	GATC	3 cut(s)	93, 190, 472
MboII	GAAGA	6 cut(s)	172, 179, 292, 467, 476, 639
MhlI	GDGCHC	2 cut(s)	180, 457
MluCI	AATT	2 cut(s)	467, 619
MlyI	GAGTC	1 cut(s)	25
MroXI	GAANNNNTTC	1 cut(s)	108
MseI	TTAA	1 cut(s)	658
MslI	CAYNNNNRTG	1 cut(s)	621
MspA1I	CMGCKG	1 cut(s)	451
MspI	CCGG	1 cut(s)	61
MvnI	CGCG	1 cut(s)	234
MwoI	GCNNNNNNNGC	1 cut(s)	21
NcoI	CCATGG	1 cut(s)	396
NdeII	GATC	3 cut(s)	93, 190, 472
NlaIII	CATG	3 cut(s)	50, 400, 463
NspI	RCATGY	1 cut(s)	50
PciI	ACATGT	1 cut(s)	46
PciSI	GCTCTTC	1 cut(s)	185
PcsI	WCGNNNNNNNCGW	1 cut(s)	171
PdmI	GAANNNNTTC	1 cut(s)	108
PkrI	GCNGC	1 cut(s)	450
Ple19I	CGATCG	1 cut(s)	193
PleI	GAGTC	1 cut(s)	25
PpsI	GAGTC	1 cut(s)	25
PpuMI	RGGWCCY	1 cut(s)	373
PscI	ACATGT	1 cut(s)	46
Psp124BI	GAGCTC	1 cut(s)	180
Psp5II	RGGWCCY	1 cut(s)	373
PspPI	GGNCC	2 cut(s)	55, 373
PspPPI	RGGWCCY	1 cut(s)	373
PvuI	CGATCG	1 cut(s)	193
RseI	CAYNNNNRTG	1 cut(s)	621
SacI	GAGCTC	1 cut(s)	180
SapI	GCTCTTC	1 cut(s)	185
SaqAI	TTAA	1 cut(s)	658
SatI	GCNGC	1 cut(s)	449
Sau3AI	GATC	3 cut(s)	93, 190, 472
Sau96I	GGNCC	2 cut(s)	55, 373
SchI	GAGTC	1 cut(s)	25
SduI	GDGCHC	2 cut(s)	180, 457
SfaNI	GCATC	3 cut(s)	304, 314, 523
SinI	GGWCC	1 cut(s)	373
SmiMI	CAYNNNNRTG	1 cut(s)	621
SmlI	CTYRAG	1 cut(s)	506
SmoI	CTYRAG	1 cut(s)	506
SpeI	ACTAGT	1 cut(s)	352
Sse9I	AATT	2 cut(s)	467, 619
SsiI	CCGC	6 cut(s)	136, 141, 147, 234, 446, 451
SspMI	CTAG	1 cut(s)	353
SstI	GAGCTC	1 cut(s)	180
StyI	CCWWGG	2 cut(s)	396, 538
TaiI	ACGT	1 cut(s)	41
TaqI	TCGA	5 cut(s)	92, 153, 165, 174, 189
TasI	AATT	2 cut(s)	467, 619
Tru1I	TTAA	1 cut(s)	658
Tru9I	TTAA	1 cut(s)	658
TscAI	CASTG	1 cut(s)	499
TseI	GCWGC	1 cut(s)	448
TspDTI	ATGAA	3 cut(s)	101, 443, 447
TspGWI	ACGGA	2 cut(s)	383, 659
TspRI	CASTG	1 cut(s)	499
VpaK11BI	GGWCC	1 cut(s)	373
XapI	RAATTY	1 cut(s)	467
XceI	RCATGY	1 cut(s)	50
XmnI	GAANNNNTTC	1 cut(s)	108
XspI	CTAG	1 cut(s)	353

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.