Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0013193

Physical Location & Seq

Genomic Coordinates Reverse (-)

5920723 .. 5922881

Gene Structure 2159 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1083700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 951 bp

ATGAGGCATCCTCAAGCATCTGAGGTCGTCATCAAGGACATAATATCACACTTTGTTCTGCAACTAGTTTATTGCACGACAGTAGATCTGAAAAAATGGTTTCTTTCCTTGGAGACTGCCTTATTTCGTTACCGATTTATAATTGAACAACCTGAACCGCAGAGGGCACTCATGGAAGAAATTGGTCTTTCATACAAGGCACTTAGTGGTTCAGAACTTGAGAATGTATTAGATAAATTGGTCCTGGTTTCACGTTCGCTCGGTGCTCAATCTCCACCAACGGCTAGTGATATCTTCCACATGGTACCGTTTGAAGAAGTTCCAGAACTGGTAGCCAGTCGTAGAGTATTCATCAGTAAGGGGCATGCCTATATAGCAAGAAATCAGGTGTTTCCCCTTGTTGCCACACGATTCCGCAGTCTTCTATCAAAGGCACTTACATTGACAAACAGAAAATGGGCAACAACTACAGCAGAACAAGAGACGGATCCGTTGCCACCTATTCTAGTCCAGTCCGAGACAGTCTGGTACAACAAACGCACCCTAAAAGACATTGATGAGGCATCCAAGACCTCATTTCCTATCTGCATGCGCCACCTATTTGACAAGTGGAGAGAAGATCATCATTTAAAGTATGGAGGGAGAATGCAGGTAGGTCTCTTTCTCAAGGGTGTTGGGTTGAAGCTGGATGATGCCCTTGCGTTTGACAAAGACTATTCATATGGCATACGCCTCAATTACGAAAGAGATGGGAAAATATCGGATTACACGCCTTGTTCCTGTCCCAAAATCATCTCATCTACTCCCGGTGTAGGAGATCATCATGGATGCCTGTATCGATATTTCAGTGCAGAGAACTTGAGAGCAGCACTAGATTTTGCAATTTGGTTTTCTATAGTGTATAAGGGGGCTGGTTTGGTATTGCTGTGCTTTGAAAAAAAACTGTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000428 GO:0003674 GO:0003676 GO:0003677 GO:0003697 GO:0003824 GO:0003887 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005658 GO:0005694 GO:0006139 GO:0006259 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030880 GO:0030894 GO:0031974 GO:0031981 GO:0032991 GO:0032993 GO:0034061 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0043601 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0055029 GO:0061695 GO:0070013 GO:0071704 GO:0071897 GO:0090304 GO:0097159 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03030 map00230 map00240 map01100 map03030

Pfam Domains

Protein Families

DNA_primase_lrg

Protein Analysis

317

Amino Acids

36.09

Weight (kDa)

8.61

Isoelectric Point (pI)

34.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_primase_lrg_N	PF26466	11 - 154	1.6e-33	Eukaryotic and archaeal DNA primase, large subunit N-terminal domain
DNA_primase_lrg	PF04104	187 - 292	8.5e-28	Eukaryotic and archaeal DNA primase, large subunit C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013193)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67320 AT1G67320
fragaria_vesca	FvH4_4g14550 FvH4_4g14550 FvH4_4g14550
malus_domestica	MD09G1083700.v1.1 MD13G1121900.v1.1 MD16G1122200.v1.1
prunus_persica	Prupe.1G223400_v2.0.a1
pyrus_communis	pycom13g10610
rosa_chinensis	RchiOBHm_Chr4g0418271
rosa_laevigata	RLG00000007881
rosa_multiflora	Rmu_sc0000032.1_g000005
rosa_roxburghii	Rroxscaffold_5G00361450
rosa_rugosa	Rorug04G0151600
rosa_samantha	Rh4AG213200 Rh4CG224900
rosa_wichuraiana	Rw4G018110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	140
Acc36I	ACCTGC	1 cut(s)	640
Acc65I	GGTACC	1 cut(s)	304
AccB1I	GGYRCC	1 cut(s)	304
AciI	CCGC	2 cut(s)	158, 415
AclWI	GGATC	2 cut(s)	482, 495
AdeI	CACNNNGTG	1 cut(s)	206
AfaI	GTAC	2 cut(s)	306, 530
AfiI	CCNNNNNNNGG	1 cut(s)	812
AgsI	TTSAA	4 cut(s)	146, 314, 682, 935
AhlI	ACTAGT	1 cut(s)	64
AjnI	CCWGG	1 cut(s)	243
AluBI	AGCT	1 cut(s)	685
AluI	AGCT	1 cut(s)	685
Alw21I	GWGCWC	1 cut(s)	268
Alw26I	GTCTC	4 cut(s)	107, 476, 512, 662
AlwI	GGATC	2 cut(s)	482, 495
ApeKI	GCWGC	1 cut(s)	866
Asp700I	GAANNNNTTC	1 cut(s)	318
Asp718I	GGTACC	1 cut(s)	304
AspLEI	GCGC	1 cut(s)	594
AspS9I	GGNCC	1 cut(s)	241
AsuC2I	CCSGG	1 cut(s)	807
AvaII	GGWCC	1 cut(s)	241
BaeGI	GKGCMC	1 cut(s)	169
BamHI	GGATCC	1 cut(s)	487
BanI	GGYRCC	1 cut(s)	304
BbsI	GAAGAC	1 cut(s)	413
Bbv12I	GWGCWC	1 cut(s)	268
BbvI	GCAGC	1 cut(s)	878
BccI	CCATC	1 cut(s)	743
BceAI	ACGGC	1 cut(s)	297
BciT130I	CCWGG	1 cut(s)	245
BcnI	CCSGG	1 cut(s)	807
BcoDI	GTCTC	4 cut(s)	107, 476, 512, 662
BcuI	ACTAGT	1 cut(s)	64
BfaI	CTAG	4 cut(s)	65, 285, 506, 872
BfmI	CTRYAG	2 cut(s)	468, 894
BfuAI	ACCTGC	1 cut(s)	640
BglII	AGATCT	1 cut(s)	85
BisI	GCNGC	1 cut(s)	867
BlsI	GCNGC	1 cut(s)	868
Bme1390I	CCNGG	2 cut(s)	245, 807
Bme18I	GGWCC	1 cut(s)	241
BmgT120I	GGNCC	1 cut(s)	241
BmiI	GGNNCC	2 cut(s)	306, 489
BmrFI	CCNGG	2 cut(s)	245, 807
BmsI	GCATC	5 cut(s)	16, 26, 572, 682, 818
BpiI	GAAGAC	1 cut(s)	413
BplI	GAGNNNNNCTC	1 cut(s)	27
BpuEI	CTTGAG	3 cut(s)	239, 650, 880
BpuMI	CCSGG	1 cut(s)	807
Bsa29I	ATCGAT	1 cut(s)	838
BsaI	GGTCTC	1 cut(s)	662
BsaJI	CCNNGG	1 cut(s)	108
Bsc4I	CCNNNNNNNGG	1 cut(s)	812
Bse1I	ACTGG	3 cut(s)	333, 336, 511
BseBI	CCWGG	1 cut(s)	245
BseCI	ATCGAT	1 cut(s)	838
BseDI	CCNNGG	1 cut(s)	108
BseGI	GGATG	4 cut(s)	7, 563, 694, 833
BseLI	CCNNNNNNNGG	1 cut(s)	812
BseMII	CTCAG	1 cut(s)	12
BseNI	ACTGG	3 cut(s)	333, 336, 511
BseSI	GKGCMC	1 cut(s)	169
BseXI	GCAGC	1 cut(s)	878
BsgI	GTGCAG	1 cut(s)	870
BshNI	GGYRCC	1 cut(s)	304
BshVI	ATCGAT	1 cut(s)	838
BsiHKAI	GWGCWC	1 cut(s)	268
BsiSI	CCGG	1 cut(s)	807
BslFI	GGGAC	1 cut(s)	768
BslI	CCNNNNNNNGG	1 cut(s)	812
BsmAI	GTCTC	4 cut(s)	107, 476, 512, 662
BsmBI	CGTCTC	1 cut(s)	476
BsmFI	GGGAC	1 cut(s)	768
BsmI	GAATGC	1 cut(s)	651
Bso31I	GGTCTC	1 cut(s)	662
Bsp1286I	GDGCHC	2 cut(s)	169, 268
Bsp143I	GATC	4 cut(s)	85, 487, 619, 817
BspACI	CCGC	2 cut(s)	158, 415
BspCNI	CTCAG	1 cut(s)	13
BspDI	ATCGAT	1 cut(s)	838
BspLI	GGNNCC	2 cut(s)	306, 489
BspMI	ACCTGC	1 cut(s)	640
BspPI	GGATC	2 cut(s)	482, 495
BspT107I	GGYRCC	1 cut(s)	304
BspTNI	GGTCTC	1 cut(s)	662
BsrI	ACTGG	3 cut(s)	333, 336, 511
BssECI	CCNNGG	1 cut(s)	108
BssMI	GATC	4 cut(s)	85, 487, 619, 817
BssT1I	CCWWGG	1 cut(s)	108
Bst2UI	CCWGG	1 cut(s)	245
Bst4CI	ACNGT	4 cut(s)	82, 309, 523, 945
BstC8I	GCNNGC	2 cut(s)	366, 590
BstDEI	CTNAG	2 cut(s)	21, 203
BstF5I	GGATG	4 cut(s)	7, 563, 694, 833
BstHHI	GCGC	1 cut(s)	594
BstKTI	GATC	4 cut(s)	88, 490, 622, 820
BstMAI	GTCTC	4 cut(s)	107, 476, 512, 662
BstMBI	GATC	4 cut(s)	85, 487, 619, 817
BstMWI	GCNNNNNNNGC	1 cut(s)	374
BstNI	CCWGG	1 cut(s)	245
BstNSI	RCATGY	2 cut(s)	368, 592
BstSCI	CCNGG	2 cut(s)	243, 805
BstSFI	CTRYAG	2 cut(s)	468, 894
BstSLI	GKGCMC	1 cut(s)	169
BstV1I	GCAGC	1 cut(s)	878
BstV2I	GAAGAC	1 cut(s)	413
BstX2I	RGATCY	2 cut(s)	85, 487
BstYI	RGATCY	2 cut(s)	85, 487
Bsu15I	ATCGAT	1 cut(s)	838
BsuTUI	ATCGAT	1 cut(s)	838
BtsCI	GGATG	4 cut(s)	7, 563, 694, 833
BtsIMutI	CAGTG	1 cut(s)	853
BveI	ACCTGC	1 cut(s)	640
Cac8I	GCNNGC	2 cut(s)	366, 590
CfoI	GCGC	1 cut(s)	594
Cfr13I	GGNCC	1 cut(s)	241
ClaI	ATCGAT	1 cut(s)	838
Csp6I	GTAC	2 cut(s)	305, 529
CviAII	CATG	5 cut(s)	172, 301, 365, 589, 824
CviJI	RGCY	4 cut(s)	284, 335, 685, 911
CviKI_1	RGCY	4 cut(s)	284, 335, 685, 911
CviQI	GTAC	2 cut(s)	305, 529
DdeI	CTNAG	2 cut(s)	21, 203
DpnI	GATC	4 cut(s)	87, 489, 621, 819
DpnII	GATC	4 cut(s)	85, 487, 619, 817
DraI	TTTAAA	1 cut(s)	630
DraIII	CACNNNGTG	1 cut(s)	206
Eco130I	CCWWGG	1 cut(s)	108
Eco31I	GGTCTC	1 cut(s)	662
Eco32I	GATATC	1 cut(s)	292
Eco47I	GGWCC	1 cut(s)	241
EcoRII	CCWGG	1 cut(s)	243
EcoRV	GATATC	1 cut(s)	292
EcoT14I	CCWWGG	1 cut(s)	108
ErhI	CCWWGG	1 cut(s)	108
Esp3I	CGTCTC	1 cut(s)	476
FaeI	CATG	5 cut(s)	175, 304, 368, 592, 827
FaqI	GGGAC	1 cut(s)	768
FatI	CATG	5 cut(s)	171, 300, 364, 588, 823
FauNDI	CATATG	1 cut(s)	721
Fnu4HI	GCNGC	1 cut(s)	867
FokI	GGATG	3 cut(s)	550, 701, 840
Fsp4HI	GCNGC	1 cut(s)	867
FspBI	CTAG	4 cut(s)	65, 285, 506, 872
GlaI	GCGC	1 cut(s)	593
GluI	GCNGC	1 cut(s)	867
HapII	CCGG	1 cut(s)	807
HhaI	GCGC	1 cut(s)	594
Hin1II	CATG	5 cut(s)	175, 304, 368, 592, 827
Hin6I	GCGC	1 cut(s)	592
HinP1I	GCGC	1 cut(s)	592
HinfI	GANTC	1 cut(s)	411
HpaII	CCGG	1 cut(s)	807
Hpy188I	TCNGA	5 cut(s)	22, 90, 214, 517, 763
Hpy188III	TCNNGA	1 cut(s)	323
HpyCH4III	ACNGT	4 cut(s)	82, 309, 523, 945
HpyCH4IV	ACGT	1 cut(s)	253
HpyCH4V	TGCA	6 cut(s)	61, 75, 588, 649, 851, 881
HpyF10VI	GCNNNNNNNGC	1 cut(s)	374
HpyF3I	CTNAG	2 cut(s)	21, 203
HpySE526I	ACGT	1 cut(s)	253
Hsp92II	CATG	5 cut(s)	175, 304, 368, 592, 827
HspAI	GCGC	1 cut(s)	592
KpnI	GGTACC	1 cut(s)	308
Kzo9I	GATC	4 cut(s)	85, 487, 619, 817
Lsp1109I	GCAGC	1 cut(s)	878
LweI	GCATC	5 cut(s)	16, 26, 572, 682, 818
MaeI	CTAG	4 cut(s)	65, 285, 506, 872
MaeII	ACGT	1 cut(s)	253
MaeIII	GTNAC	1 cut(s)	128
MalI	GATC	4 cut(s)	87, 489, 621, 819
MboI	GATC	4 cut(s)	85, 487, 619, 817
MboII	GAAGA	5 cut(s)	188, 286, 326, 413, 629
MflI	RGATCY	2 cut(s)	85, 487
MhlI	GDGCHC	2 cut(s)	169, 268
MluCI	AATT	5 cut(s)	141, 180, 236, 736, 882
MnlI	CCTC	7 cut(s)	16, 21, 156, 553, 583, 632, 743
MroXI	GAANNNNTTC	1 cut(s)	318
MseI	TTAA	1 cut(s)	629
MspI	CCGG	1 cut(s)	807
MspR9I	CCNGG	2 cut(s)	245, 807
Mva1269I	GAATGC	1 cut(s)	651
MvaI	CCWGG	1 cut(s)	245
MwoI	GCNNNNNNNGC	1 cut(s)	374
NciI	CCSGG	1 cut(s)	807
NdeI	CATATG	1 cut(s)	721
NdeII	GATC	4 cut(s)	85, 487, 619, 817
NlaIII	CATG	5 cut(s)	175, 304, 368, 592, 827
NlaIV	GGNNCC	2 cut(s)	306, 489
NspI	RCATGY	2 cut(s)	368, 592
PaeI	GCATGC	2 cut(s)	368, 592
PctI	GAATGC	1 cut(s)	651
PdmI	GAANNNNTTC	1 cut(s)	318
PfeI	GAWTC	1 cut(s)	411
PkrI	GCNGC	1 cut(s)	868
PsiI	TTATAA	1 cut(s)	140
Psp6I	CCWGG	1 cut(s)	243
PspGI	CCWGG	1 cut(s)	243
PspN4I	GGNNCC	2 cut(s)	306, 489
PspPI	GGNCC	1 cut(s)	241
PsuI	RGATCY	2 cut(s)	85, 487
RsaI	GTAC	2 cut(s)	306, 530
RsaNI	GTAC	2 cut(s)	305, 529
SaqAI	TTAA	1 cut(s)	629
SatI	GCNGC	1 cut(s)	867
Sau3AI	GATC	4 cut(s)	85, 487, 619, 817
Sau96I	GGNCC	1 cut(s)	241
ScrFI	CCNGG	2 cut(s)	245, 807
SduI	GDGCHC	2 cut(s)	169, 268
SfaNI	GCATC	5 cut(s)	16, 26, 572, 682, 818
SfcI	CTRYAG	2 cut(s)	468, 894
SinI	GGWCC	1 cut(s)	241
SmlI	CTYRAG	4 cut(s)	12, 218, 665, 859
SmoI	CTYRAG	4 cut(s)	12, 218, 665, 859
SpeI	ACTAGT	1 cut(s)	64
SphI	GCATGC	2 cut(s)	368, 592
Sse9I	AATT	5 cut(s)	141, 180, 236, 736, 882
SsiI	CCGC	2 cut(s)	158, 415
SspMI	CTAG	4 cut(s)	65, 285, 506, 872
StyD4I	CCNGG	2 cut(s)	243, 805
StyI	CCWWGG	1 cut(s)	108
TaaI	ACNGT	4 cut(s)	82, 309, 523, 945
TaiI	ACGT	1 cut(s)	256
TaqI	TCGA	1 cut(s)	838
TasI	AATT	5 cut(s)	141, 180, 236, 736, 882
TfiI	GAWTC	1 cut(s)	411
Tru1I	TTAA	1 cut(s)	629
Tru9I	TTAA	1 cut(s)	629
TscAI	CASTG	1 cut(s)	853
TseI	GCWGC	1 cut(s)	866
TspDTI	ATGAA	3 cut(s)	180, 340, 708
TspGWI	ACGGA	2 cut(s)	480, 500
TspRI	CASTG	1 cut(s)	853
VpaK11BI	GGWCC	1 cut(s)	241
XceI	RCATGY	2 cut(s)	368, 592
XmnI	GAANNNNTTC	1 cut(s)	318
XspI	CTAG	4 cut(s)	65, 285, 506, 872

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.