FvH4_4g19670

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0019700
Physical Location & Seq
Forward (+)
23243430 .. 23243891
462 bp
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UTR
Exon/CDS
Intron
FvH4_4g19670.t1

Sequence Viewer

Length: 300 bp
ATGGTCTACACTAACCCTGAAATAGCCGCTTTGTCCGCACTGAAGAGTAGCTTATCTGATCCAGACAATGAGCTCAAGTCAAACAACATTGAAGGAGTTATTCCAGAAGAGCTTGGTAAGCTGACGAACCTCATAGGCTTGGACTTGTTTGAAAACATCCTCTCGGGGTTCTTACCTTCTTCATTGGAGAATTTGAAATCACTTCGGATTCTGCGTCTCAATGGTAACAGGTTCCTCACTGGTGCCGTCCCCCGAGTCTTGTTAACGTTCCCGTCCTCGACATCTCGAACACAAGTCTAA

Protein Analysis

100

Amino Acids

10.78

Weight (kDa)

5.31

Isoelectric Point (pI)

41.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 25 - 78 8.6e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019700)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g19670
malus_domestica MD00G1124100.v1.1 MD04G1039800.v1.1
prunus_persica Prupe.4G284500_v2.0.a1 Prupe.4G284500_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0484661
rosa_multiflora Rmu_sc0007498.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 242
AccI GTMKAC 1 cut(s) 6
AciI CCGC 2 cut(s) 27, 36
AclI AACGTT 1 cut(s) 266
AclWI GGATC 1 cut(s) 53
AcsI RAATTY 1 cut(s) 190
AcuI CTGAAG 1 cut(s) 62
AgsI TTSAA 3 cut(s) 92, 152, 196
AluBI AGCT 4 cut(s) 51, 73, 112, 121
AluI AGCT 4 cut(s) 51, 73, 112, 121
Alw21I GWGCWC 1 cut(s) 75
Alw26I GTCTC 1 cut(s) 221
AlwI GGATC 1 cut(s) 53
Ama87I CYCGRG 2 cut(s) 163, 252
ApoI RAATTY 1 cut(s) 190
AvaI CYCGRG 2 cut(s) 163, 252
BanI GGYRCC 1 cut(s) 242
BanII GRGCYC 1 cut(s) 75
Bbv12I GWGCWC 1 cut(s) 75
BceAI ACGGC 1 cut(s) 230
BcoDI GTCTC 1 cut(s) 221
BisI GCNGC 1 cut(s) 27
BlsI GCNGC 1 cut(s) 28
BmeT110I CYCGRG 2 cut(s) 163, 252
BmiI GGNNCC 2 cut(s) 233, 244
BpuEI CTTGAG 1 cut(s) 59
Bse1I ACTGG 1 cut(s) 244
BseGI GGATG 1 cut(s) 156
BseNI ACTGG 1 cut(s) 244
BshNI GGYRCC 1 cut(s) 242
BsiHKAI GWGCWC 1 cut(s) 75
BsiHKCI CYCGRG 2 cut(s) 163, 252
BslFI GGGAC 1 cut(s) 233
BsmAI GTCTC 1 cut(s) 221
BsmBI CGTCTC 1 cut(s) 221
BsmFI GGGAC 1 cut(s) 233
BsoBI CYCGRG 2 cut(s) 163, 252
Bsp1286I GDGCHC 1 cut(s) 75
Bsp143I GATC 1 cut(s) 58
BspACI CCGC 2 cut(s) 27, 36
BspLI GGNNCC 2 cut(s) 233, 244
BspPI GGATC 1 cut(s) 53
BspQI GCTCTTC 1 cut(s) 102
BspT107I GGYRCC 1 cut(s) 242
BsrI ACTGG 1 cut(s) 244
BssMI GATC 1 cut(s) 58
Bst6I CTCTTC 2 cut(s) 38, 102
BstF5I GGATG 1 cut(s) 156
BstKTI GATC 1 cut(s) 61
BstMAI GTCTC 1 cut(s) 221
BstMBI GATC 1 cut(s) 58
BstMWI GCNNNNNNNGC 2 cut(s) 35, 118
BtsCI GGATG 1 cut(s) 156
BtsIMutI CAGTG 2 cut(s) 38, 237
CseI GACGC 1 cut(s) 203
CviJI RGCY 6 cut(s) 26, 51, 73, 112, 121, 138
CviKI_1 RGCY 6 cut(s) 26, 51, 73, 112, 121, 138
DpnI GATC 1 cut(s) 60
DpnII GATC 1 cut(s) 58
Eam1104I CTCTTC 2 cut(s) 38, 102
EarI CTCTTC 2 cut(s) 38, 102
Ecl136II GAGCTC 1 cut(s) 73
Eco24I GRGCYC 1 cut(s) 75
Eco53kI GAGCTC 1 cut(s) 73
Eco57I CTGAAG 1 cut(s) 62
Eco88I CYCGRG 2 cut(s) 163, 252
EcoICRI GAGCTC 1 cut(s) 73
EcoT38I GRGCYC 1 cut(s) 75
Esp3I CGTCTC 1 cut(s) 221
FaiI YATR 1 cut(s) 134
FalI AAGNNNNNCTT 2 cut(s) 35, 67
FaqI GGGAC 1 cut(s) 233
FblI GTMKAC 1 cut(s) 6
Fnu4HI GCNGC 1 cut(s) 27
FokI GGATG 1 cut(s) 143
FriOI GRGCYC 1 cut(s) 75
Fsp4HI GCNGC 1 cut(s) 27
GluI GCNGC 1 cut(s) 27
HgaI GACGC 1 cut(s) 203
HincII GTYRAC 1 cut(s) 264
HindII GTYRAC 1 cut(s) 264
HinfI GANTC 2 cut(s) 208, 255
HpaI GTTAAC 1 cut(s) 264
Hpy166II GTNNAC 2 cut(s) 7, 264
Hpy188I TCNGA 2 cut(s) 58, 207
Hpy188III TCNNGA 3 cut(s) 62, 104, 285
Hpy8I GTNNAC 2 cut(s) 7, 264
HpyAV CCTTC 2 cut(s) 86, 186
HpyCH4IV ACGT 1 cut(s) 266
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 118
HpySE526I ACGT 1 cut(s) 266
KspAI GTTAAC 1 cut(s) 264
Kzo9I GATC 1 cut(s) 58
LguI GCTCTTC 1 cut(s) 102
LpnPI CCDG 5 cut(s) 30, 75, 117, 214, 225
MaeII ACGT 1 cut(s) 266
MaeIII GTNAC 1 cut(s) 224
MalI GATC 1 cut(s) 60
MboI GATC 1 cut(s) 58
MboII GAAGA 3 cut(s) 55, 119, 171
MhlI GDGCHC 1 cut(s) 75
MluCI AATT 1 cut(s) 190
MlyI GAGTC 1 cut(s) 264
MnlI CCTC 4 cut(s) 140, 170, 245, 286
MseI TTAA 1 cut(s) 263
MwoI GCNNNNNNNGC 2 cut(s) 35, 118
NdeII GATC 1 cut(s) 58
NlaIV GGNNCC 2 cut(s) 233, 244
PciSI GCTCTTC 1 cut(s) 102
PcsI WCGNNNNNNNCGW 1 cut(s) 211
PfeI GAWTC 1 cut(s) 208
PkrI GCNGC 1 cut(s) 28
PleI GAGTC 1 cut(s) 263
PpsI GAGTC 1 cut(s) 263
Psp124BI GAGCTC 1 cut(s) 75
Psp1406I AACGTT 1 cut(s) 266
PspN4I GGNNCC 2 cut(s) 233, 244
SacI GAGCTC 1 cut(s) 75
SapI GCTCTTC 1 cut(s) 102
SaqAI TTAA 1 cut(s) 263
SatI GCNGC 1 cut(s) 27
Sau3AI GATC 1 cut(s) 58
SchI GAGTC 1 cut(s) 264
SduI GDGCHC 1 cut(s) 75
SetI ASST 8 cut(s) 53, 75, 114, 123, 132, 178, 233, 269
SmlI CTYRAG 1 cut(s) 74
SmoI CTYRAG 1 cut(s) 74
Sse9I AATT 1 cut(s) 190
SsiI CCGC 2 cut(s) 27, 36
SstI GAGCTC 1 cut(s) 75
TaiI ACGT 1 cut(s) 269
TaqI TCGA 2 cut(s) 278, 286
TasI AATT 1 cut(s) 190
TauI GCSGC 1 cut(s) 29
TfiI GAWTC 1 cut(s) 208
Tru1I TTAA 1 cut(s) 263
Tru9I TTAA 1 cut(s) 263
TscAI CASTG 2 cut(s) 45, 244
TspDTI ATGAA 1 cut(s) 171
TspRI CASTG 2 cut(s) 45, 244
XapI RAATTY 1 cut(s) 190
XmiI GTMKAC 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.