MD04G1039800.v1.1

LRR receptor-like serine threonine-protein kinase At4g08850

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0019700
Physical Location & Seq
Forward (+)
4366511 .. 4367095
585 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1039800.v1.1.491

Sequence Viewer

Length: 585 bp
ATGCACATTTTCGGATCACTGCCCCTTTTCGGCTTTCCCTTATTTCCAAATCTCACCCACTTCAATCTAAGTGGCAACAATTTCCAAGGGCCTATACCGCATGCCATTGGAAATCTCTCCAGGCTCACGTCTTTGGACTTGGGCAACAACTTTTTTGATCAACAAATTCCATCTGAGATTGACCAGGTAACAGAGCTTCAGTATCTAAATCTCCGCAACAACCGTCTCTCTGGTACCATCCCTTATCAACTGAGCAATCTCCCAAAGGTATGGTACATGGACCTTGGATTAAACTATTTTTTCCCTGCTGATCAAGATTGGTCAGAATTTGCAGGCATGCCTTCATTGACCTACCTTGCTATGTCTATGCAACGTGATAATCTATATCATTCAGAATTCCCAGAATTTATATCTCAATGCCGGAACTTGACGTTCCTCAACTTGTCTCATAATAATTTGAATGGCCAAATTCCACCTTCGATAGGCCAACTCAGGGAGCTCCAGTACCTTGATCTTTCATACAACTCCTTAAACTCTTCTATCCCTTCTGAGCTTGGTCTTTGTACAAAACCTCACCCCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_8 Pkinase RVT_2

Protein Analysis

195

Amino Acids

22.1

Weight (kDa)

5.15

Isoelectric Point (pI)

47.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 134 - 177 7.8e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019700)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g19670
malus_domestica MD00G1124100.v1.1 MD04G1039800.v1.1
prunus_persica Prupe.4G284500_v2.0.a1 Prupe.4G284500_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0484661
rosa_multiflora Rmu_sc0007498.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 233
AccB1I GGYRCC 1 cut(s) 233
AciI CCGC 2 cut(s) 98, 214
AclWI GGATC 1 cut(s) 22
AcoI YGGCCR 1 cut(s) 463
AcsI RAATTY 5 cut(s) 165, 326, 395, 404, 468
AcuI CTGAAG 1 cut(s) 182
AfaI GTAC 4 cut(s) 235, 275, 506, 565
AfiI CCNNNNNNNGG 3 cut(s) 29, 482, 493
AgsI TTSAA 2 cut(s) 64, 460
AjiI CACGTC 1 cut(s) 129
AjnI CCWGG 2 cut(s) 119, 183
AluBI AGCT 3 cut(s) 196, 499, 553
AluI AGCT 3 cut(s) 196, 499, 553
Alw21I GWGCWC 1 cut(s) 501
Alw26I GTCTC 2 cut(s) 230, 450
AlwI GGATC 1 cut(s) 22
AoxI GGCC 3 cut(s) 89, 463, 484
ApoI RAATTY 5 cut(s) 165, 326, 395, 404, 468
Asp718I GGTACC 1 cut(s) 233
AspS9I GGNCC 2 cut(s) 89, 280
AsuHPI GGTGA 2 cut(s) 46, 566
AvaII GGWCC 1 cut(s) 280
BalI TGGCCA 1 cut(s) 465
BanI GGYRCC 1 cut(s) 233
BanII GRGCYC 1 cut(s) 501
BarI GAAGNNNNNNTAC 2 cut(s) 180, 212
Bbv12I GWGCWC 1 cut(s) 501
BccI CCATC 2 cut(s) 178, 245
BciT130I CCWGG 2 cut(s) 121, 185
BclI TGATCA 2 cut(s) 157, 310
BcoDI GTCTC 2 cut(s) 230, 450
Bme1390I CCNGG 2 cut(s) 121, 185
Bme18I GGWCC 1 cut(s) 280
BmgBI CACGTC 1 cut(s) 129
BmgT120I GGNCC 2 cut(s) 89, 280
BmiI GGNNCC 1 cut(s) 235
BmrFI CCNGG 2 cut(s) 121, 185
BpmI CTGGAG 2 cut(s) 103, 485
BsaJI CCNNGG 2 cut(s) 85, 283
BsaXI ACNNNNNCTCC 2 cut(s) 488, 518
Bsc4I CCNNNNNNNGG 3 cut(s) 29, 482, 493
Bse1I ACTGG 1 cut(s) 502
BseBI CCWGG 2 cut(s) 121, 185
BseDI CCNNGG 2 cut(s) 85, 283
BseGI GGATG 1 cut(s) 237
BseLI CCNNNNNNNGG 3 cut(s) 29, 482, 493
BseMII CTCAG 4 cut(s) 165, 242, 505, 540
BseNI ACTGG 1 cut(s) 502
BshFI GGCC 3 cut(s) 91, 465, 486
BshNI GGYRCC 1 cut(s) 233
BsiHKAI GWGCWC 1 cut(s) 501
BsiSI CCGG 1 cut(s) 421
BslI CCNNNNNNNGG 3 cut(s) 29, 482, 493
BsmAI GTCTC 2 cut(s) 230, 450
BsmBI CGTCTC 1 cut(s) 230
BsnI GGCC 3 cut(s) 91, 465, 486
Bsp1286I GDGCHC 1 cut(s) 501
Bsp1407I TGTACA 1 cut(s) 563
Bsp143I GATC 4 cut(s) 14, 157, 310, 511
BspACI CCGC 2 cut(s) 98, 214
BspANI GGCC 3 cut(s) 91, 465, 486
BspCNI CTCAG 4 cut(s) 166, 243, 504, 541
BspLI GGNNCC 1 cut(s) 235
BspPI GGATC 1 cut(s) 22
BspT107I GGYRCC 1 cut(s) 233
BsrGI TGTACA 1 cut(s) 563
BsrI ACTGG 1 cut(s) 502
BssECI CCNNGG 2 cut(s) 85, 283
BssMI GATC 4 cut(s) 14, 157, 310, 511
BssT1I CCWWGG 2 cut(s) 85, 283
Bst2UI CCWGG 2 cut(s) 121, 185
Bst4CI ACNGT 1 cut(s) 224
Bst6I CTCTTC 1 cut(s) 541
BstAUI TGTACA 1 cut(s) 563
BstC8I GCNNGC 3 cut(s) 102, 334, 338
BstDEI CTNAG 5 cut(s) 68, 174, 251, 491, 549
BstENI CCTNNNNNAGG 1 cut(s) 480
BstF5I GGATG 1 cut(s) 237
BstKTI GATC 4 cut(s) 17, 160, 313, 514
BstMAI GTCTC 2 cut(s) 230, 450
BstMBI GATC 4 cut(s) 14, 157, 310, 511
BstMWI GCNNNNNNNGC 1 cut(s) 97
BstNI CCWGG 2 cut(s) 121, 185
BstNSI RCATGY 2 cut(s) 104, 340
BstSCI CCNGG 2 cut(s) 119, 183
BstXI CCANNNNNNTGG 1 cut(s) 270
BsuRI GGCC 3 cut(s) 91, 465, 486
BtrI CACGTC 1 cut(s) 129
BtsCI GGATG 1 cut(s) 237
BtsI GCAGTG 1 cut(s) 17
BtsIMutI CAGTG 1 cut(s) 17
Cac8I GCNNGC 3 cut(s) 102, 334, 338
Cfr13I GGNCC 2 cut(s) 89, 280
CsiI ACCWGGT 1 cut(s) 183
Csp6I GTAC 4 cut(s) 234, 274, 505, 564
CspCI CAANNNNNGTGG 2 cut(s) 52, 87
CviAII CATG 3 cut(s) 101, 277, 337
CviJI RGCY 8 cut(s) 33, 91, 124, 196, 465, 486, 499, 553
CviKI_1 RGCY 8 cut(s) 33, 91, 124, 196, 465, 486, 499, 553
CviQI GTAC 4 cut(s) 234, 274, 505, 564
DdeI CTNAG 5 cut(s) 68, 174, 251, 491, 549
DpnI GATC 4 cut(s) 16, 159, 312, 513
DpnII GATC 4 cut(s) 14, 157, 310, 511
EaeI YGGCCR 1 cut(s) 463
Eam1104I CTCTTC 1 cut(s) 541
EarI CTCTTC 1 cut(s) 541
Ecl136II GAGCTC 1 cut(s) 499
Eco130I CCWWGG 2 cut(s) 85, 283
Eco24I GRGCYC 1 cut(s) 501
Eco47I GGWCC 1 cut(s) 280
Eco53kI GAGCTC 1 cut(s) 499
Eco57I CTGAAG 1 cut(s) 182
EcoICRI GAGCTC 1 cut(s) 499
EcoNI CCTNNNNNAGG 1 cut(s) 480
EcoO109I RGGNCCY 1 cut(s) 89
EcoRI GAATTC 1 cut(s) 395
EcoRII CCWGG 2 cut(s) 119, 183
EcoT14I CCWWGG 2 cut(s) 85, 283
EcoT38I GRGCYC 1 cut(s) 501
ErhI CCWWGG 2 cut(s) 85, 283
Esp3I CGTCTC 1 cut(s) 230
FaeI CATG 3 cut(s) 104, 280, 340
FatI CATG 3 cut(s) 100, 276, 336
FbaI TGATCA 2 cut(s) 157, 310
FokI GGATG 1 cut(s) 224
FriOI GRGCYC 1 cut(s) 501
GsuI CTGGAG 2 cut(s) 103, 485
HaeIII GGCC 3 cut(s) 91, 465, 486
HapII CCGG 1 cut(s) 421
Hin1II CATG 3 cut(s) 104, 280, 340
HpaII CCGG 1 cut(s) 421
HphI GGTGA 2 cut(s) 46, 566
Hpy188I TCNGA 5 cut(s) 14, 175, 325, 394, 550
Hpy188III TCNNGA 1 cut(s) 314
HpyAV CCTTC 3 cut(s) 351, 486, 555
HpyCH4III ACNGT 1 cut(s) 224
HpyCH4IV ACGT 3 cut(s) 128, 373, 431
HpyCH4V TGCA 3 cut(s) 4, 332, 370
HpyF10VI GCNNNNNNNGC 1 cut(s) 97
HpyF3I CTNAG 5 cut(s) 68, 174, 251, 491, 549
HpySE526I ACGT 3 cut(s) 128, 373, 431
Hsp92II CATG 3 cut(s) 104, 280, 340
KpnI GGTACC 1 cut(s) 237
Ksp22I TGATCA 2 cut(s) 157, 310
Kzo9I GATC 4 cut(s) 14, 157, 310, 511
LmnI GCTCC 2 cut(s) 496, 504
MabI ACCWGGT 1 cut(s) 183
MaeII ACGT 3 cut(s) 128, 373, 431
MaeIII GTNAC 1 cut(s) 187
MalI GATC 4 cut(s) 16, 159, 312, 513
MboI GATC 4 cut(s) 14, 157, 310, 511
MboII GAAGA 1 cut(s) 528
MhlI GDGCHC 1 cut(s) 501
MlsI TGGCCA 1 cut(s) 465
MluCI AATT 7 cut(s) 79, 165, 326, 395, 404, 454, 468
MluNI TGGCCA 1 cut(s) 465
MnlI CCTC 2 cut(s) 446, 582
Mox20I TGGCCA 1 cut(s) 465
MscI TGGCCA 1 cut(s) 465
MseI TTAA 2 cut(s) 290, 530
Msp20I TGGCCA 1 cut(s) 465
MspI CCGG 1 cut(s) 421
MspR9I CCNGG 2 cut(s) 121, 185
MvaI CCWGG 2 cut(s) 121, 185
MwoI GCNNNNNNNGC 1 cut(s) 97
NdeII GATC 4 cut(s) 14, 157, 310, 511
NlaIII CATG 3 cut(s) 104, 280, 340
NlaIV GGNNCC 1 cut(s) 235
NspI RCATGY 2 cut(s) 104, 340
PaeI GCATGC 2 cut(s) 104, 340
Psp124BI GAGCTC 1 cut(s) 501
Psp6I CCWGG 2 cut(s) 119, 183
PspGI CCWGG 2 cut(s) 119, 183
PspN4I GGNNCC 1 cut(s) 235
PspPI GGNCC 2 cut(s) 89, 280
RsaI GTAC 4 cut(s) 235, 275, 506, 565
RsaNI GTAC 4 cut(s) 234, 274, 505, 564
SacI GAGCTC 1 cut(s) 501
SaqAI TTAA 2 cut(s) 290, 530
Sau3AI GATC 4 cut(s) 14, 157, 310, 511
Sau96I GGNCC 2 cut(s) 89, 280
ScrFI CCNGG 2 cut(s) 121, 185
SduI GDGCHC 1 cut(s) 501
SexAI ACCWGGT 1 cut(s) 183
SinI GGWCC 1 cut(s) 280
SphI GCATGC 2 cut(s) 104, 340
Sse9I AATT 7 cut(s) 79, 165, 326, 395, 404, 454, 468
SsiI CCGC 2 cut(s) 98, 214
SstI GAGCTC 1 cut(s) 501
StyD4I CCNGG 2 cut(s) 119, 183
StyI CCWWGG 2 cut(s) 85, 283
TaaI ACNGT 1 cut(s) 224
TaiI ACGT 3 cut(s) 131, 376, 434
TaqI TCGA 1 cut(s) 479
TasI AATT 7 cut(s) 79, 165, 326, 395, 404, 454, 468
TatI WGTACW 1 cut(s) 563
Tru1I TTAA 2 cut(s) 290, 530
Tru9I TTAA 2 cut(s) 290, 530
TscAI CASTG 1 cut(s) 24
TspDTI ATGAA 2 cut(s) 333, 507
TspRI CASTG 1 cut(s) 24
VpaK11BI GGWCC 1 cut(s) 280
XagI CCTNNNNNAGG 1 cut(s) 480
XapI RAATTY 5 cut(s) 165, 326, 395, 404, 468
XceI RCATGY 2 cut(s) 104, 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.