MD00G1124100.v1.1

LRR receptor-like serine threonine-protein kinase At4g08850

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0019700
Physical Location & Seq
Forward (+)
26602949 .. 26604713
1765 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1124100.v1.1.491

Sequence Viewer

Length: 1638 bp
ATGCACATTTTCGGATCACTGCCCCTTTTCGGCTTTCCCTTATTTCCAAATCTCACCCACTTCAATCTAAGTGGCAACAATTTCCAAGGGCCTATACCGCATGCCATTGGAAATCTCTCCAGGCTCACGTCTTTGGACTTGGGCAACAACTTTTTTGATCAACAAATTCCATCTGAGATTGACCAGGTAACAGAGCTTCAGTATCTAAATCTCCGCAACAACCGTCTCTCTGGTACCATCCCTTATCAACTGAGCAATCTCCCAAAGAATTCCCAGAATTTATATCTCAATGCCGGAAACTTGACGTTCCTCAACTTGTCTCATAATAATTTGAATGGCCAAATTCCACCTTCGATAGGCCAACTCAGGGAGCTCCAGTACCTTGATCTTTCATACAACTCCTTAAACTCTTCTATCCCTTCTGAGCTTGGTCTTTGTACAAACCTCACCCACTTGAGTTTAGCTAATAACCGATTCTCTGGCACGATTCCGGACAATATAGGTTTGATTTCTGGTCTTCAACGTGTTGACCTGAGTATGAATCTTCTGGAAGGGAAAATTCCATCCTCTGTAGGCCAACTCAGGGAGCTCCAGTACCTTGATCTTTCATCCAACTCCTTAACCTCAACAATCCCTTCTGAACTTGTTCTTTGTACAAACCTCACCTACTTGAGTTTAGCTAATAACCGATTTTCTGGCACGATTCCGGACAATATAGGTTTGATTTCTGGTCTTCAACGTGTTGACCTGAGTATAAATCTTCTGGAAGGGAAAATTCCATCCTCTGTAGGCCAACTCAGGGAGCTCCAGTACCTTGATCTTTCATCCAACTCCTTATACTCAACAATCCCTTCTGAACTTGTTCTTTGTACAAACCTCACCTACTTGTACTTGTCTTCCAATAAACTCAATGGGGAACTGCCTCTGTCCTTGTCCAATCTGAACAACCTCGTCGAATTGGGTTTAGCAAAGAACCAATTCTCTGGCACGATTTCGGACAATATAGGTTTGATTTCTGGTCTTCAACATATTGACCTGGGAATGAATCTTCTGGAAGGGGAAATTCCACTCTCTATAGGCCAACTCAGGGAGCTCCAGTACCTAAATCTTTCATACAACTTCTTAAACTCATCAATCCCTTCTGAACTTGGTCTTTGTACAAACCTCAACCGCTTGTCCTTGTCTTCCAATAAACTCAACGGGGAACTGCCTTTGTCCTTGTCCAATCTGAACAACCTCGTCGAATTGGGTTTAGATAATAATCTATTTACTGGTCAAATCCTTCCTTCTCTGGTCTCCAATTGGACTAATATGGAATCTTTGCAACTTCAGTCCAATGAACTCAGTGGGGAGATTCCGACGGAAGTTGGAAATATGAGCTTTCTGTTCACGCTCAATCTAAGAAGGAACCATTTGACAGGAGTGATCCCTCAGATTCTAGGCAATTTAATTTATCTTAAGCTGCTCGATTTGTCACACAACCATCTTTCGGGGCAAATCCCATCATTTGTCAACATATATTTTTTCTTAATGCTTTTAATTTTTCTTATAACAACTTGTCTGGCCGAATCCCATGCGTTTTCCAAAGGGCATCAAAAAATCCTTTTGTTGGAAACTCTGGCTTGTGTGGCTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_8 Pkinase RVT_2

Protein Analysis

546

Amino Acids

59.98

Weight (kDa)

5.12

Isoelectric Point (pI)

37.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 16 - 76 1.3e-07 Leucine rich repeat
LRR_8 PF13855 100 - 159 1.9e-06 Leucine rich repeat
LRR_14 PF23598 100 - 177 3.2e-06 Leucine-rich repeat region
LRR_8 PF13855 147 - 207 1.2e-06 Leucine rich repeat
LRR_8 PF13855 172 - 231 9.9e-08 Leucine rich repeat
LRR_8 PF13855 219 - 279 1.3e-06 Leucine rich repeat
LRR_8 PF13855 244 - 303 1.2e-07 Leucine rich repeat
LRR_14 PF23598 258 - 344 5.7e-07 Leucine-rich repeat region
LRR_14 PF23598 358 - 467 2e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019700)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g19670
malus_domestica MD00G1124100.v1.1 MD04G1039800.v1.1
prunus_persica Prupe.4G284500_v2.0.a1 Prupe.4G284500_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0484661
rosa_multiflora Rmu_sc0007498.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1550
Acc65I GGTACC 1 cut(s) 233
AccB1I GGYRCC 1 cut(s) 233
AccIII TCCGGA 2 cut(s) 490, 706
AciI CCGC 3 cut(s) 98, 214, 1171
AclWI GGATC 2 cut(s) 22, 1420
AcoI YGGCCR 2 cut(s) 337, 1563
AcsI RAATTY 7 cut(s) 165, 268, 277, 342, 558, 774, 1062
AcuI CTGAAG 2 cut(s) 182, 1313
AfiI CCNNNNNNNGG 8 cut(s) 29, 356, 367, 583, 799, 1087, 1489, 1609
AflII CTTAAG 1 cut(s) 1457
AflIII ACRYGT 2 cut(s) 523, 739
AgsI TTSAA 5 cut(s) 64, 334, 521, 737, 1025
AjiI CACGTC 1 cut(s) 129
AjnI CCWGG 3 cut(s) 119, 183, 1035
Alw21I GWGCWC 4 cut(s) 375, 591, 807, 1095
Alw26I GTCTC 3 cut(s) 230, 324, 1300
AlwI GGATC 2 cut(s) 22, 1420
Aor13HI TCCGGA 2 cut(s) 490, 706
AoxI GGCC 7 cut(s) 89, 337, 358, 574, 790, 1078, 1563
ApeKI GCWGC 1 cut(s) 1462
ApoI RAATTY 7 cut(s) 165, 268, 277, 342, 558, 774, 1062
Asp700I GAANNNNTTC 3 cut(s) 645, 861, 977
Asp718I GGTACC 1 cut(s) 233
AspS9I GGNCC 1 cut(s) 89
AsuHPI GGTGA 4 cut(s) 46, 439, 655, 871
BalI TGGCCA 1 cut(s) 339
BanI GGYRCC 1 cut(s) 233
BanII GRGCYC 4 cut(s) 375, 591, 807, 1095
BarI GAAGNNNNNNTAC 2 cut(s) 180, 212
BbsI GAAGAC 5 cut(s) 509, 725, 888, 1013, 1176
Bbv12I GWGCWC 4 cut(s) 375, 591, 807, 1095
BbvI GCAGC 1 cut(s) 1449
BccI CCATC 6 cut(s) 178, 245, 571, 787, 1491, 1510
BcgI CGANNNNNNTGC 2 cut(s) 1556, 1590
BciT130I CCWGG 3 cut(s) 121, 185, 1037
BclI TGATCA 1 cut(s) 157
BcoDI GTCTC 3 cut(s) 230, 324, 1300
BfaI CTAG 1 cut(s) 1439
BfmI CTRYAG 3 cut(s) 570, 786, 1074
BfrI CTTAAG 1 cut(s) 1457
BisI GCNGC 1 cut(s) 1463
BlsI GCNGC 1 cut(s) 1464
Bme1390I CCNGG 3 cut(s) 121, 185, 1037
BmgBI CACGTC 1 cut(s) 129
BmgT120I GGNCC 1 cut(s) 89
BmiI GGNNCC 2 cut(s) 235, 1409
BmrFI CCNGG 3 cut(s) 121, 185, 1037
BmsI GCATC 1 cut(s) 1600
BpiI GAAGAC 5 cut(s) 509, 725, 888, 1013, 1176
BpmI CTGGAG 5 cut(s) 103, 359, 575, 791, 1079
BpuEI CTTGAG 2 cut(s) 475, 691
BsaBI GATNNNNATC 1 cut(s) 1260
BsaI GGTCTC 1 cut(s) 1300
BsaJI CCNNGG 2 cut(s) 85, 1036
BsaWI WCCGGW 2 cut(s) 490, 706
BsaXI ACNNNNNCTCC 8 cut(s) 362, 392, 578, 608, 794, 824, 1082, 1112
Bsc4I CCNNNNNNNGG 8 cut(s) 29, 356, 367, 583, 799, 1087, 1489, 1609
Bse1I ACTGG 5 cut(s) 376, 592, 808, 1096, 1276
Bse8I GATNNNNATC 1 cut(s) 1260
BseAI TCCGGA 2 cut(s) 490, 706
BseBI CCWGG 3 cut(s) 121, 185, 1037
BseDI CCNNGG 2 cut(s) 85, 1036
BseGI GGATG 5 cut(s) 237, 563, 608, 779, 824
BseJI GATNNNNATC 1 cut(s) 1260
BseLI CCNNNNNNNGG 8 cut(s) 29, 356, 367, 583, 799, 1087, 1489, 1609
BseNI ACTGG 5 cut(s) 376, 592, 808, 1096, 1276
BseXI GCAGC 1 cut(s) 1449
BshFI GGCC 7 cut(s) 91, 339, 360, 576, 792, 1080, 1565
BshNI GGYRCC 1 cut(s) 233
BsiHKAI GWGCWC 4 cut(s) 375, 591, 807, 1095
BsiSI CCGG 3 cut(s) 294, 491, 707
BslI CCNNNNNNNGG 8 cut(s) 29, 356, 367, 583, 799, 1087, 1489, 1609
BsmAI GTCTC 3 cut(s) 230, 324, 1300
BsmBI CGTCTC 1 cut(s) 230
BsnI GGCC 7 cut(s) 91, 339, 360, 576, 792, 1080, 1565
Bso31I GGTCTC 1 cut(s) 1300
Bsp1286I GDGCHC 4 cut(s) 375, 591, 807, 1095
Bsp13I TCCGGA 2 cut(s) 490, 706
Bsp1407I TGTACA 4 cut(s) 437, 653, 869, 1157
Bsp143I GATC 6 cut(s) 14, 157, 385, 601, 817, 1425
BspACI CCGC 3 cut(s) 98, 214, 1171
BspANI GGCC 7 cut(s) 91, 339, 360, 576, 792, 1080, 1565
BspEI TCCGGA 2 cut(s) 490, 706
BspLI GGNNCC 2 cut(s) 235, 1409
BspPI GGATC 2 cut(s) 22, 1420
BspT107I GGYRCC 1 cut(s) 233
BspTI CTTAAG 1 cut(s) 1457
BspTNI GGTCTC 1 cut(s) 1300
BsrGI TGTACA 4 cut(s) 437, 653, 869, 1157
BsrI ACTGG 5 cut(s) 376, 592, 808, 1096, 1276
BssECI CCNNGG 2 cut(s) 85, 1036
BssMI GATC 6 cut(s) 14, 157, 385, 601, 817, 1425
BssT1I CCWWGG 1 cut(s) 85
Bst2UI CCWGG 3 cut(s) 121, 185, 1037
Bst4CI ACNGT 1 cut(s) 224
Bst6I CTCTTC 1 cut(s) 415
BstAFI CTTAAG 1 cut(s) 1457
BstAUI TGTACA 4 cut(s) 437, 653, 869, 1157
BstC8I GCNNGC 1 cut(s) 102
BstENI CCTNNNNNAGG 1 cut(s) 354
BstF5I GGATG 5 cut(s) 237, 563, 608, 779, 824
BstKTI GATC 6 cut(s) 17, 160, 388, 604, 820, 1428
BstMAI GTCTC 3 cut(s) 230, 324, 1300
BstMBI GATC 6 cut(s) 14, 157, 385, 601, 817, 1425
BstMWI GCNNNNNNNGC 2 cut(s) 97, 1628
BstNI CCWGG 3 cut(s) 121, 185, 1037
BstNSI RCATGY 1 cut(s) 104
BstSCI CCNGG 3 cut(s) 119, 183, 1035
BstSFI CTRYAG 3 cut(s) 570, 786, 1074
BstV1I GCAGC 1 cut(s) 1449
BstV2I GAAGAC 5 cut(s) 509, 725, 888, 1013, 1176
BstXI CCANNNNNNTGG 1 cut(s) 983
BsuRI GGCC 7 cut(s) 91, 339, 360, 576, 792, 1080, 1565
BtrI CACGTC 1 cut(s) 129
BtsCI GGATG 5 cut(s) 237, 563, 608, 779, 824
BtsI GCAGTG 1 cut(s) 17
BtsIMutI CAGTG 2 cut(s) 17, 1351
Cac8I GCNNGC 1 cut(s) 102
Cfr13I GGNCC 1 cut(s) 89
CsiI ACCWGGT 1 cut(s) 183
CspCI CAANNNNNGTGG 2 cut(s) 52, 87
CviAII CATG 2 cut(s) 101, 1574
DpnI GATC 6 cut(s) 16, 159, 387, 603, 819, 1427
DpnII GATC 6 cut(s) 14, 157, 385, 601, 817, 1425
EaeI YGGCCR 2 cut(s) 337, 1563
Eam1104I CTCTTC 1 cut(s) 415
EarI CTCTTC 1 cut(s) 415
Ecl136II GAGCTC 4 cut(s) 373, 589, 805, 1093
Eco130I CCWWGG 1 cut(s) 85
Eco24I GRGCYC 4 cut(s) 375, 591, 807, 1095
Eco31I GGTCTC 1 cut(s) 1300
Eco53kI GAGCTC 4 cut(s) 373, 589, 805, 1093
Eco57I CTGAAG 2 cut(s) 182, 1313
EcoICRI GAGCTC 4 cut(s) 373, 589, 805, 1093
EcoNI CCTNNNNNAGG 1 cut(s) 354
EcoO109I RGGNCCY 1 cut(s) 89
EcoRI GAATTC 1 cut(s) 268
EcoRII CCWGG 3 cut(s) 119, 183, 1035
EcoT14I CCWWGG 1 cut(s) 85
EcoT38I GRGCYC 4 cut(s) 375, 591, 807, 1095
ErhI CCWWGG 1 cut(s) 85
Esp3I CGTCTC 1 cut(s) 230
FaeI CATG 2 cut(s) 104, 1577
FatI CATG 2 cut(s) 100, 1573
FbaI TGATCA 1 cut(s) 157
Fnu4HI GCNGC 1 cut(s) 1463
FokI GGATG 5 cut(s) 224, 550, 595, 766, 811
FriOI GRGCYC 4 cut(s) 375, 591, 807, 1095
Fsp4HI GCNGC 1 cut(s) 1463
FspBI CTAG 1 cut(s) 1439
GluI GCNGC 1 cut(s) 1463
GsuI CTGGAG 5 cut(s) 103, 359, 575, 791, 1079
HaeIII GGCC 7 cut(s) 91, 339, 360, 576, 792, 1080, 1565
HapII CCGG 3 cut(s) 294, 491, 707
Hin1II CATG 2 cut(s) 104, 1577
HincII GTYRAC 3 cut(s) 529, 745, 1513
HindII GTYRAC 3 cut(s) 529, 745, 1513
HinfI GANTC 9 cut(s) 474, 487, 541, 703, 1045, 1316, 1354, 1435, 1568
HpaII CCGG 3 cut(s) 294, 491, 707
HphI GGTGA 4 cut(s) 46, 439, 655, 871
Hpy166II GTNNAC 4 cut(s) 529, 745, 1389, 1513
Hpy188III TCNNGA 5 cut(s) 491, 548, 707, 764, 1052
Hpy8I GTNNAC 4 cut(s) 529, 745, 1389, 1513
Hpy99I CGWCG 3 cut(s) 956, 1244, 1363
HpyCH4III ACNGT 1 cut(s) 224
HpyCH4IV ACGT 4 cut(s) 128, 305, 523, 739
HpyCH4V TGCA 2 cut(s) 4, 1324
HpyF10VI GCNNNNNNNGC 2 cut(s) 97, 1628
HpySE526I ACGT 4 cut(s) 128, 305, 523, 739
Hsp92II CATG 2 cut(s) 104, 1577
Kpn2I TCCGGA 2 cut(s) 490, 706
KpnI GGTACC 1 cut(s) 237
Ksp22I TGATCA 1 cut(s) 157
Kzo9I GATC 6 cut(s) 14, 157, 385, 601, 817, 1425
LmnI GCTCC 8 cut(s) 370, 378, 586, 594, 802, 810, 1090, 1098
Lsp1109I GCAGC 1 cut(s) 1449
LweI GCATC 1 cut(s) 1600
MabI ACCWGGT 1 cut(s) 183
MaeI CTAG 1 cut(s) 1439
MaeII ACGT 4 cut(s) 128, 305, 523, 739
MaeIII GTNAC 2 cut(s) 187, 1473
MalI GATC 6 cut(s) 16, 159, 387, 603, 819, 1427
MboI GATC 6 cut(s) 14, 157, 385, 601, 817, 1425
MboII GAAGA 9 cut(s) 402, 509, 536, 725, 752, 888, 1013, 1040, 1176
MfeI CAATTG 1 cut(s) 1300
MhlI GDGCHC 4 cut(s) 375, 591, 807, 1095
MlsI TGGCCA 1 cut(s) 339
MluNI TGGCCA 1 cut(s) 339
MmeI TCCRAC 5 cut(s) 636, 852, 1348, 1382, 1590
Mox20I TGGCCA 1 cut(s) 339
MroI TCCGGA 2 cut(s) 490, 706
MroXI GAANNNNTTC 3 cut(s) 645, 861, 977
MscI TGGCCA 1 cut(s) 339
MseI TTAA 7 cut(s) 404, 620, 1124, 1448, 1458, 1529, 1538
Msp20I TGGCCA 1 cut(s) 339
MspCI CTTAAG 1 cut(s) 1457
MspI CCGG 3 cut(s) 294, 491, 707
MspR9I CCNGG 3 cut(s) 121, 185, 1037
MunI CAATTG 1 cut(s) 1300
MvaI CCWGG 3 cut(s) 121, 185, 1037
MwoI GCNNNNNNNGC 2 cut(s) 97, 1628
NdeII GATC 6 cut(s) 14, 157, 385, 601, 817, 1425
NlaIII CATG 2 cut(s) 104, 1577
NlaIV GGNNCC 2 cut(s) 235, 1409
NmuCI GTSAC 1 cut(s) 1473
NspI RCATGY 1 cut(s) 104
PaeI GCATGC 1 cut(s) 104
PdmI GAANNNNTTC 3 cut(s) 645, 861, 977
PfeI GAWTC 9 cut(s) 474, 487, 541, 703, 1045, 1316, 1354, 1435, 1568
PkrI GCNGC 1 cut(s) 1464
PsiI TTATAA 1 cut(s) 1550
Psp124BI GAGCTC 4 cut(s) 375, 591, 807, 1095
Psp6I CCWGG 3 cut(s) 119, 183, 1035
PspGI CCWGG 3 cut(s) 119, 183, 1035
PspN4I GGNNCC 2 cut(s) 235, 1409
PspPI GGNCC 1 cut(s) 89
SacI GAGCTC 4 cut(s) 375, 591, 807, 1095
SaqAI TTAA 7 cut(s) 404, 620, 1124, 1448, 1458, 1529, 1538
SatI GCNGC 1 cut(s) 1463
Sau3AI GATC 6 cut(s) 14, 157, 385, 601, 817, 1425
Sau96I GGNCC 1 cut(s) 89
ScrFI CCNGG 3 cut(s) 121, 185, 1037
SduI GDGCHC 4 cut(s) 375, 591, 807, 1095
SexAI ACCWGGT 1 cut(s) 183
SfaNI GCATC 1 cut(s) 1600
SfcI CTRYAG 3 cut(s) 570, 786, 1074
SmlI CTYRAG 3 cut(s) 454, 670, 1457
SmoI CTYRAG 3 cut(s) 454, 670, 1457
SphI GCATGC 1 cut(s) 104
SsiI CCGC 3 cut(s) 98, 214, 1171
SspMI CTAG 1 cut(s) 1439
SstI GAGCTC 4 cut(s) 375, 591, 807, 1095
StyD4I CCNGG 3 cut(s) 119, 183, 1035
StyI CCWWGG 1 cut(s) 85
TaaI ACNGT 1 cut(s) 224
TaiI ACGT 4 cut(s) 131, 308, 526, 742
TaqI TCGA 4 cut(s) 353, 954, 1242, 1467
TatI WGTACW 5 cut(s) 437, 653, 869, 888, 1157
TfiI GAWTC 9 cut(s) 474, 487, 541, 703, 1045, 1316, 1354, 1435, 1568
Tru1I TTAA 7 cut(s) 404, 620, 1124, 1448, 1458, 1529, 1538
Tru9I TTAA 7 cut(s) 404, 620, 1124, 1448, 1458, 1529, 1538
TscAI CASTG 2 cut(s) 24, 1351
TseFI GTSAC 1 cut(s) 1473
TseI GCWGC 1 cut(s) 1462
Tsp45I GTSAC 1 cut(s) 1473
TspDTI ATGAA 7 cut(s) 381, 554, 597, 813, 1058, 1101, 1353
TspGWI ACGGA 1 cut(s) 1376
TspRI CASTG 2 cut(s) 24, 1351
Vha464I CTTAAG 1 cut(s) 1457
XagI CCTNNNNNAGG 1 cut(s) 354
XapI RAATTY 7 cut(s) 165, 268, 277, 342, 558, 774, 1062
XceI RCATGY 1 cut(s) 104
XmnI GAANNNNTTC 3 cut(s) 645, 861, 977
XspI CTAG 1 cut(s) 1439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.