FvH4_4g21930

female pronucleus assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0016205
Physical Location & Seq
Forward (+)
24904158 .. 24908730
4573 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g21930.t1

Sequence Viewer

Length: 615 bp
ATGGCGTTGAATCCTCAGCTCTTACCAAACGGCATGCCGGTTCCTTTCGTCAACGAGATGTTCGTCTTGATCAGAGACGGCGTCGAGTTCGAAGTCGATAAGATTCCCGGATCCGATGGAGGTCGGCTCAAAGCAAAGGGGACAATTTACCTGTCAAATATCCGCATGGTATTTGTTTCCAAGAAGCCTGTTGGTGATTTCGTTGCTTTTGATATGCCCCTGCTTTTTGTCCATGGAGAAAAATTTAATCAACCGGTCTTTTTCTGCAACAACATAGCTGGCCAGGTGGAGCCTGTGGTTCCTGAAAACCAGCCTGCGGCTCTTTATTCTGTCCACTCATTTAAGATTTTGTTCAAAGATGGGGGTTGTGGGACTTTTGTTCCACTGTTTTTCAACTTGATATCCTCAGTGAGGCAATATAATCAGCAATTTCAACAAGCCAATACCCCACAGGCTCATGTGGATCCTTTACAGGCAGCACAAACTCCAGTTGATGAGATGATGAGACATGCGTATGTTGATCCTAATGATCCAACGAGGATCTTCTTGCAGCAGCCTACTCCAGAGTCTCAGCTGAGGCGGCGTACGTATCAGCCACAAGCTGCCAATCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

205

Amino Acids

22.96

Weight (kDa)

6.51

Isoelectric Point (pI)

45.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016205)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G11680
fragaria_vesca FvH4_4g21930
malus_domestica MD13G1135700.v1.1 MD16G1131300.v1.1
prunus_persica Prupe.1G209600_v2.0.a1
pyrus_communis pycom16g11570
rosa_chinensis RchiOBHm_Chr4g0428141
rosa_laevigata RLG00000007176
rosa_multiflora Rmu_sc0004940.1_g000010 Rmu_sc0009599.1_g000010
rosa_roxburghii Rroxscaffold_5G00369870
rosa_rugosa Rorug04G0222900
rosa_samantha Rh4BG285700 Rh4CG301000
rosa_wichuraiana Rw4G024330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 163, 317, 580
AclWI GGATC 7 cut(s) 105, 118, 458, 471, 515, 524, 548
AcoI YGGCCR 1 cut(s) 280
AcsI RAATTY 1 cut(s) 242
AcyI GRCGYC 1 cut(s) 81
AfaI GTAC 1 cut(s) 586
AfiI CCNNNNNNNGG 2 cut(s) 316, 411
AgeI ACCGGT 1 cut(s) 253
AgsI TTSAA 4 cut(s) 10, 355, 394, 434
AjnI CCWGG 1 cut(s) 282
AluBI AGCT 4 cut(s) 19, 278, 574, 602
AluI AGCT 4 cut(s) 19, 278, 574, 602
Alw26I GTCTC 3 cut(s) 69, 499, 573
AlwI GGATC 7 cut(s) 105, 118, 458, 471, 515, 524, 548
AoxI GGCC 1 cut(s) 280
ApeKI GCWGC 4 cut(s) 476, 550, 553, 602
ApoI RAATTY 1 cut(s) 242
AsiGI ACCGGT 1 cut(s) 253
AsuC2I CCSGG 1 cut(s) 108
AsuHPI GGTGA 1 cut(s) 206
AsuII TTCGAA 1 cut(s) 90
BalI TGGCCA 1 cut(s) 282
BamHI GGATCC 2 cut(s) 110, 463
BbvCI CCTCAGC 2 cut(s) 15, 575
BbvI GCAGC 4 cut(s) 488, 562, 565, 589
BccI CCATC 2 cut(s) 110, 353
BceAI ACGGC 2 cut(s) 46, 94
BciT130I CCWGG 1 cut(s) 284
BclI TGATCA 1 cut(s) 69
BcnI CCSGG 1 cut(s) 108
BcoDI GTCTC 3 cut(s) 69, 499, 573
BfaI CTAG 1 cut(s) 613
BisI GCNGC 6 cut(s) 318, 477, 551, 554, 581, 603
BlsI GCNGC 6 cut(s) 319, 478, 552, 555, 582, 604
Bme1390I CCNGG 2 cut(s) 108, 284
BmiI GGNNCC 5 cut(s) 42, 112, 291, 300, 465
BmrFI CCNGG 2 cut(s) 108, 284
BplI GAGNNNNNCTC 2 cut(s) 111, 143
BpmI CTGGAG 2 cut(s) 471, 546
Bpu10I CCTNAGC 2 cut(s) 15, 575
Bpu14I TTCGAA 1 cut(s) 90
BpuMI CCSGG 1 cut(s) 108
BsaAI YACGTR 1 cut(s) 588
BsaHI GRCGYC 1 cut(s) 81
BsaJI CCNNGG 1 cut(s) 232
BsaWI WCCGGW 1 cut(s) 253
Bsc4I CCNNNNNNNGG 2 cut(s) 316, 411
Bse118I RCCGGY 2 cut(s) 37, 253
Bse1I ACTGG 1 cut(s) 488
BseBI CCWGG 1 cut(s) 284
BseDI CCNNGG 1 cut(s) 232
BseLI CCNNNNNNNGG 2 cut(s) 316, 411
BseMII CTCAG 4 cut(s) 29, 420, 566, 584
BseNI ACTGG 1 cut(s) 488
BseXI GCAGC 4 cut(s) 488, 562, 565, 589
BshFI GGCC 1 cut(s) 282
BshTI ACCGGT 1 cut(s) 253
BsiSI CCGG 3 cut(s) 38, 108, 254
BsiWI CGTACG 1 cut(s) 584
BslFI GGGAC 2 cut(s) 154, 385
BslI CCNNNNNNNGG 2 cut(s) 316, 411
BsmAI GTCTC 3 cut(s) 69, 499, 573
BsmBI CGTCTC 1 cut(s) 69
BsmFI GGGAC 2 cut(s) 154, 385
BsnI GGCC 1 cut(s) 282
Bsp119I TTCGAA 1 cut(s) 90
Bsp143I GATC 6 cut(s) 69, 110, 463, 520, 529, 540
Bsp19I CCATGG 1 cut(s) 232
BspACI CCGC 3 cut(s) 163, 317, 580
BspANI GGCC 1 cut(s) 282
BspCNI CTCAG 4 cut(s) 28, 419, 567, 583
BspLI GGNNCC 5 cut(s) 42, 112, 291, 300, 465
BspPI GGATC 7 cut(s) 105, 118, 458, 471, 515, 524, 548
BspT104I TTCGAA 1 cut(s) 90
BsrFI RCCGGY 2 cut(s) 37, 253
BsrI ACTGG 1 cut(s) 488
BssAI RCCGGY 2 cut(s) 37, 253
BssECI CCNNGG 1 cut(s) 232
BssMI GATC 6 cut(s) 69, 110, 463, 520, 529, 540
BssNI GRCGYC 1 cut(s) 81
BssT1I CCWWGG 1 cut(s) 232
Bst2UI CCWGG 1 cut(s) 284
Bst4CI ACNGT 1 cut(s) 387
BstACI GRCGYC 1 cut(s) 81
BstBAI YACGTR 1 cut(s) 588
BstBI TTCGAA 1 cut(s) 90
BstC8I GCNNGC 3 cut(s) 35, 280, 315
BstDEI CTNAG 4 cut(s) 15, 406, 570, 575
BstDSI CCRYGG 1 cut(s) 232
BstENI CCTNNNNNAGG 1 cut(s) 409
BstKTI GATC 6 cut(s) 72, 113, 466, 523, 532, 543
BstMAI GTCTC 3 cut(s) 69, 499, 573
BstMBI GATC 6 cut(s) 69, 110, 463, 520, 529, 540
BstMWI GCNNNNNNNGC 1 cut(s) 580
BstNI CCWGG 1 cut(s) 284
BstNSI RCATGY 2 cut(s) 37, 512
BstSCI CCNGG 2 cut(s) 106, 282
BstSNI TACGTA 1 cut(s) 588
BstV1I GCAGC 4 cut(s) 488, 562, 565, 589
BstX2I RGATCY 3 cut(s) 110, 463, 540
BstYI RGATCY 3 cut(s) 110, 463, 540
BsuRI GGCC 1 cut(s) 282
BtgI CCRYGG 1 cut(s) 232
BtsIMutI CAGTG 2 cut(s) 383, 414
Cac8I GCNNGC 3 cut(s) 35, 280, 315
Cfr10I RCCGGY 2 cut(s) 37, 253
CseI GACGC 1 cut(s) 70
Csp6I GTAC 1 cut(s) 585
CspAI ACCGGT 1 cut(s) 253
CviAII CATG 5 cut(s) 34, 166, 233, 458, 509
CviQI GTAC 1 cut(s) 585
DdeI CTNAG 4 cut(s) 15, 406, 570, 575
DpnI GATC 6 cut(s) 71, 112, 465, 522, 531, 542
DpnII GATC 6 cut(s) 69, 110, 463, 520, 529, 540
EaeI YGGCCR 1 cut(s) 280
Eco105I TACGTA 1 cut(s) 588
Eco130I CCWWGG 1 cut(s) 232
Eco32I GATATC 1 cut(s) 402
EcoNI CCTNNNNNAGG 1 cut(s) 409
EcoRII CCWGG 1 cut(s) 282
EcoRV GATATC 1 cut(s) 402
EcoT14I CCWWGG 1 cut(s) 232
ErhI CCWWGG 1 cut(s) 232
Esp3I CGTCTC 1 cut(s) 69
FaeI CATG 5 cut(s) 37, 169, 236, 461, 512
FaiI YATR 9 cut(s) 35, 167, 215, 234, 275, 420, 459, 510, 516
FaqI GGGAC 2 cut(s) 154, 385
FatI CATG 5 cut(s) 33, 165, 232, 457, 508
FbaI TGATCA 1 cut(s) 69
Fnu4HI GCNGC 6 cut(s) 318, 477, 551, 554, 581, 603
Fsp4HI GCNGC 6 cut(s) 318, 477, 551, 554, 581, 603
FspBI CTAG 1 cut(s) 613
GluI GCNGC 6 cut(s) 318, 477, 551, 554, 581, 603
GsuI CTGGAG 2 cut(s) 471, 546
HaeIII GGCC 1 cut(s) 282
HapII CCGG 3 cut(s) 38, 108, 254
HgaI GACGC 1 cut(s) 70
Hin1I GRCGYC 1 cut(s) 81
Hin1II CATG 5 cut(s) 37, 169, 236, 461, 512
HincII GTYRAC 1 cut(s) 52
HindII GTYRAC 1 cut(s) 52
HinfI GANTC 3 cut(s) 10, 103, 566
HpaII CCGG 3 cut(s) 38, 108, 254
HphI GGTGA 1 cut(s) 206
Hpy166II GTNNAC 2 cut(s) 52, 334
Hpy188I TCNGA 2 cut(s) 74, 115
Hpy188III TCNNGA 3 cut(s) 67, 302, 563
Hpy8I GTNNAC 2 cut(s) 52, 334
Hpy99I CGWCG 1 cut(s) 86
HpyCH4III ACNGT 1 cut(s) 387
HpyCH4IV ACGT 1 cut(s) 587
HpyCH4V TGCA 2 cut(s) 267, 550
HpyF10VI GCNNNNNNNGC 1 cut(s) 580
HpyF3I CTNAG 4 cut(s) 15, 406, 570, 575
HpySE526I ACGT 1 cut(s) 587
Hsp92I GRCGYC 1 cut(s) 81
Hsp92II CATG 5 cut(s) 37, 169, 236, 461, 512
Ksp22I TGATCA 1 cut(s) 69
Kzo9I GATC 6 cut(s) 69, 110, 463, 520, 529, 540
LmnI GCTCC 1 cut(s) 289
Lsp1109I GCAGC 4 cut(s) 488, 562, 565, 589
MaeI CTAG 1 cut(s) 613
MaeII ACGT 1 cut(s) 587
MalI GATC 6 cut(s) 71, 112, 465, 522, 531, 542
MboI GATC 6 cut(s) 69, 110, 463, 520, 529, 540
MboII GAAGA 1 cut(s) 535
MflI RGATCY 3 cut(s) 110, 463, 540
MlsI TGGCCA 1 cut(s) 282
MluCI AATT 3 cut(s) 144, 242, 428
MluNI TGGCCA 1 cut(s) 282
MlyI GAGTC 1 cut(s) 575
MmeI TCCRAC 1 cut(s) 557
MnlI CCTC 6 cut(s) 24, 113, 405, 415, 531, 570
Mox20I TGGCCA 1 cut(s) 282
MscI TGGCCA 1 cut(s) 282
MseI TTAA 2 cut(s) 246, 342
MslI CAYNNNNRTG 1 cut(s) 513
Msp20I TGGCCA 1 cut(s) 282
MspA1I CMGCKG 1 cut(s) 574
MspI CCGG 3 cut(s) 38, 108, 254
MspR9I CCNGG 2 cut(s) 108, 284
MvaI CCWGG 1 cut(s) 284
MwoI GCNNNNNNNGC 1 cut(s) 580
NciI CCSGG 1 cut(s) 108
NcoI CCATGG 1 cut(s) 232
NdeII GATC 6 cut(s) 69, 110, 463, 520, 529, 540
NlaIII CATG 5 cut(s) 37, 169, 236, 461, 512
NlaIV GGNNCC 5 cut(s) 42, 112, 291, 300, 465
NspI RCATGY 2 cut(s) 37, 512
NspV TTCGAA 1 cut(s) 90
PaeI GCATGC 1 cut(s) 37
PcsI WCGNNNNNNNCGW 1 cut(s) 60
PfeI GAWTC 2 cut(s) 10, 103
Pfl23II CGTACG 1 cut(s) 584
PflFI GACNNNGTC 1 cut(s) 80
PfoI TCCNGGA 1 cut(s) 106
PinAI ACCGGT 1 cut(s) 253
PkrI GCNGC 6 cut(s) 319, 478, 552, 555, 582, 604
PleI GAGTC 1 cut(s) 574
PpsI GAGTC 1 cut(s) 574
Ppu21I YACGTR 1 cut(s) 588
Psp6I CCWGG 1 cut(s) 282
PspGI CCWGG 1 cut(s) 282
PspLI CGTACG 1 cut(s) 584
PspN4I GGNNCC 5 cut(s) 42, 112, 291, 300, 465
PsuI RGATCY 3 cut(s) 110, 463, 540
PsyI GACNNNGTC 1 cut(s) 80
PvuII CAGCTG 1 cut(s) 574
RsaI GTAC 1 cut(s) 586
RsaNI GTAC 1 cut(s) 585
RseI CAYNNNNRTG 1 cut(s) 513
SaqAI TTAA 2 cut(s) 246, 342
SatI GCNGC 6 cut(s) 318, 477, 551, 554, 581, 603
Sau3AI GATC 6 cut(s) 69, 110, 463, 520, 529, 540
SchI GAGTC 1 cut(s) 575
ScrFI CCNGG 2 cut(s) 108, 284
SetI ASST 8 cut(s) 21, 124, 153, 280, 288, 576, 590, 604
SfuI TTCGAA 1 cut(s) 90
SmiMI CAYNNNNRTG 1 cut(s) 513
SnaBI TACGTA 1 cut(s) 588
SphI GCATGC 1 cut(s) 37
Sse9I AATT 3 cut(s) 144, 242, 428
SsiI CCGC 3 cut(s) 163, 317, 580
SspMI CTAG 1 cut(s) 613
StyD4I CCNGG 2 cut(s) 106, 282
StyI CCWWGG 1 cut(s) 232
TaaI ACNGT 1 cut(s) 387
TaiI ACGT 1 cut(s) 590
TaqI TCGA 3 cut(s) 84, 90, 96
TasI AATT 3 cut(s) 144, 242, 428
TauI GCSGC 2 cut(s) 320, 583
TfiI GAWTC 2 cut(s) 10, 103
Tru1I TTAA 2 cut(s) 246, 342
Tru9I TTAA 2 cut(s) 246, 342
TscAI CASTG 2 cut(s) 390, 414
TseI GCWGC 4 cut(s) 476, 550, 553, 602
TspRI CASTG 2 cut(s) 390, 414
Tth111I GACNNNGTC 1 cut(s) 80
XagI CCTNNNNNAGG 1 cut(s) 409
XapI RAATTY 1 cut(s) 242
XceI RCATGY 2 cut(s) 37, 512
XspI CTAG 1 cut(s) 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.