RchiOBHm_Chr4g0428141

female pronucleus assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0016205
Physical Location & Seq
Forward (+)
53225451 .. 53228572
3122 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39705

Sequence Viewer

Length: 615 bp
ATGGCGTTGAATCCTCAGCTGTTACCTAACGGCATGCCGGTTCCTTTCGTCAACGAGATGTTCGTCTTGATCAGAGACGGCGTCGAGTTCGAAGTCGATAAGATTCCCGGATCCGATGGAGGTCGTGTTAAAGCAAAGGGAACAATTTACTTGTCAAATATCCGCATGGTCTTTGTTTCCAAGAAGCCTGATGGTTATTTCACTGCTTTTGATATGCCCCTGCTTTATGTCCATGGAGAGAAATTTAACCAACCGGTCTTTTTCTGCAACAATATAGCTGGTCAGGTGGAGCCTGTGGTTCCAGAAAACCAGCCTGCGGCTCTTTATTCTGTCCACTCATTCAAGATTTTGTTCAAAGACGGGGGCTGTGGGACTTTTGTTCCTCTGTTTTTCAACTTACTCTCATCTGTGAGGCGATATAATCAACAATTTCAACAAGCCAATGCCCCACAGGCTTATGTGGATCCTCTACAAGCAGCACAAACTCCGGTTGATGAGATGATGAGACATGCGTATGTTGATCCTAATGATCCAACGAGAATCTTCTTGCAGCAGCCTACTCCAGAGTCTCAGCTGAGGCGGCGTACATACCAGCCACAAGCTGCCAATCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

204

Amino Acids

23.05

Weight (kDa)

6.83

Isoelectric Point (pI)

48.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016205)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G11680
fragaria_vesca FvH4_4g21930
malus_domestica MD13G1135700.v1.1 MD16G1131300.v1.1
prunus_persica Prupe.1G209600_v2.0.a1
pyrus_communis pycom16g11570
rosa_chinensis RchiOBHm_Chr4g0428141
rosa_laevigata RLG00000007176
rosa_multiflora Rmu_sc0004940.1_g000010 Rmu_sc0009599.1_g000010
rosa_roxburghii Rroxscaffold_5G00369870
rosa_rugosa Rorug04G0222900
rosa_samantha Rh4BG285700 Rh4CG301000
rosa_wichuraiana Rw4G024330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 163, 317, 580
AclWI GGATC 6 cut(s) 105, 118, 458, 471, 515, 524
AcsI RAATTY 1 cut(s) 242
AcyI GRCGYC 1 cut(s) 81
AfaI GTAC 1 cut(s) 586
AfiI CCNNNNNNNGG 1 cut(s) 316
AgeI ACCGGT 1 cut(s) 253
AgsI TTSAA 5 cut(s) 10, 343, 355, 394, 434
AluBI AGCT 4 cut(s) 19, 278, 574, 602
AluI AGCT 4 cut(s) 19, 278, 574, 602
Alw26I GTCTC 3 cut(s) 69, 499, 573
AlwI GGATC 6 cut(s) 105, 118, 458, 471, 515, 524
ApeKI GCWGC 4 cut(s) 476, 550, 553, 602
ApoI RAATTY 1 cut(s) 242
AsiGI ACCGGT 1 cut(s) 253
AsuC2I CCSGG 1 cut(s) 108
AsuII TTCGAA 1 cut(s) 90
BamHI GGATCC 2 cut(s) 110, 463
BbvCI CCTCAGC 2 cut(s) 15, 575
BbvI GCAGC 4 cut(s) 488, 562, 565, 589
BccI CCATC 2 cut(s) 110, 185
BceAI ACGGC 2 cut(s) 46, 94
BclI TGATCA 1 cut(s) 69
BcnI CCSGG 1 cut(s) 108
BcoDI GTCTC 3 cut(s) 69, 499, 573
BglI GCCNNNNNGGC 1 cut(s) 452
BisI GCNGC 6 cut(s) 318, 477, 551, 554, 581, 603
BlsI GCNGC 6 cut(s) 319, 478, 552, 555, 582, 604
Bme1390I CCNGG 1 cut(s) 108
BmiI GGNNCC 5 cut(s) 42, 112, 291, 300, 465
BmrFI CCNGG 1 cut(s) 108
BpmI CTGGAG 1 cut(s) 546
Bpu10I CCTNAGC 2 cut(s) 15, 575
Bpu14I TTCGAA 1 cut(s) 90
BpuMI CCSGG 1 cut(s) 108
BsaHI GRCGYC 1 cut(s) 81
BsaJI CCNNGG 1 cut(s) 232
BsaWI WCCGGW 2 cut(s) 253, 487
BsaXI ACNNNNNCTCC 2 cut(s) 111, 141
Bsc4I CCNNNNNNNGG 1 cut(s) 316
Bse118I RCCGGY 2 cut(s) 37, 253
BseDI CCNNGG 1 cut(s) 232
BseLI CCNNNNNNNGG 1 cut(s) 316
BseMII CTCAG 3 cut(s) 29, 566, 584
BseXI GCAGC 4 cut(s) 488, 562, 565, 589
BshTI ACCGGT 1 cut(s) 253
BsiSI CCGG 4 cut(s) 38, 108, 254, 488
BslFI GGGAC 1 cut(s) 385
BslI CCNNNNNNNGG 1 cut(s) 316
BsmAI GTCTC 3 cut(s) 69, 499, 573
BsmBI CGTCTC 1 cut(s) 69
BsmFI GGGAC 1 cut(s) 385
Bsp119I TTCGAA 1 cut(s) 90
Bsp143I GATC 5 cut(s) 69, 110, 463, 520, 529
Bsp19I CCATGG 1 cut(s) 232
BspACI CCGC 3 cut(s) 163, 317, 580
BspCNI CTCAG 3 cut(s) 28, 567, 583
BspLI GGNNCC 5 cut(s) 42, 112, 291, 300, 465
BspPI GGATC 6 cut(s) 105, 118, 458, 471, 515, 524
BspT104I TTCGAA 1 cut(s) 90
BsrFI RCCGGY 2 cut(s) 37, 253
BssAI RCCGGY 2 cut(s) 37, 253
BssECI CCNNGG 1 cut(s) 232
BssMI GATC 5 cut(s) 69, 110, 463, 520, 529
BssNI GRCGYC 1 cut(s) 81
BssT1I CCWWGG 1 cut(s) 232
BstACI GRCGYC 1 cut(s) 81
BstBI TTCGAA 1 cut(s) 90
BstC8I GCNNGC 2 cut(s) 35, 315
BstDEI CTNAG 3 cut(s) 15, 570, 575
BstDSI CCRYGG 1 cut(s) 232
BstKTI GATC 5 cut(s) 72, 113, 466, 523, 532
BstMAI GTCTC 3 cut(s) 69, 499, 573
BstMBI GATC 5 cut(s) 69, 110, 463, 520, 529
BstMWI GCNNNNNNNGC 2 cut(s) 452, 580
BstNSI RCATGY 2 cut(s) 37, 512
BstSCI CCNGG 1 cut(s) 106
BstV1I GCAGC 4 cut(s) 488, 562, 565, 589
BstX2I RGATCY 2 cut(s) 110, 463
BstYI RGATCY 2 cut(s) 110, 463
BtgI CCRYGG 1 cut(s) 232
BtsI GCAGTG 1 cut(s) 201
BtsIMutI CAGTG 1 cut(s) 201
Cac8I GCNNGC 2 cut(s) 35, 315
Cfr10I RCCGGY 2 cut(s) 37, 253
CseI GACGC 1 cut(s) 70
Csp6I GTAC 1 cut(s) 585
CspAI ACCGGT 1 cut(s) 253
CviAII CATG 4 cut(s) 34, 166, 233, 509
CviQI GTAC 1 cut(s) 585
DdeI CTNAG 3 cut(s) 15, 570, 575
DpnI GATC 5 cut(s) 71, 112, 465, 522, 531
DpnII GATC 5 cut(s) 69, 110, 463, 520, 529
Eco130I CCWWGG 1 cut(s) 232
EcoT14I CCWWGG 1 cut(s) 232
ErhI CCWWGG 1 cut(s) 232
Esp3I CGTCTC 1 cut(s) 69
FaeI CATG 4 cut(s) 37, 169, 236, 512
FaqI GGGAC 1 cut(s) 385
FatI CATG 4 cut(s) 33, 165, 232, 508
FbaI TGATCA 1 cut(s) 69
Fnu4HI GCNGC 6 cut(s) 318, 477, 551, 554, 581, 603
Fsp4HI GCNGC 6 cut(s) 318, 477, 551, 554, 581, 603
GluI GCNGC 6 cut(s) 318, 477, 551, 554, 581, 603
GsuI CTGGAG 1 cut(s) 546
HapII CCGG 4 cut(s) 38, 108, 254, 488
HgaI GACGC 1 cut(s) 70
Hin1I GRCGYC 1 cut(s) 81
Hin1II CATG 4 cut(s) 37, 169, 236, 512
HincII GTYRAC 1 cut(s) 52
HindII GTYRAC 1 cut(s) 52
HinfI GANTC 4 cut(s) 10, 103, 540, 566
HpaII CCGG 4 cut(s) 38, 108, 254, 488
Hpy166II GTNNAC 2 cut(s) 52, 334
Hpy188I TCNGA 2 cut(s) 74, 115
Hpy188III TCNNGA 4 cut(s) 67, 302, 343, 563
Hpy8I GTNNAC 2 cut(s) 52, 334
Hpy99I CGWCG 1 cut(s) 86
HpyCH4V TGCA 2 cut(s) 267, 550
HpyF10VI GCNNNNNNNGC 2 cut(s) 452, 580
HpyF3I CTNAG 3 cut(s) 15, 570, 575
Hsp92I GRCGYC 1 cut(s) 81
Hsp92II CATG 4 cut(s) 37, 169, 236, 512
Ksp22I TGATCA 1 cut(s) 69
Kzo9I GATC 5 cut(s) 69, 110, 463, 520, 529
LmnI GCTCC 1 cut(s) 289
Lsp1109I GCAGC 4 cut(s) 488, 562, 565, 589
MaeIII GTNAC 1 cut(s) 21
MalI GATC 5 cut(s) 71, 112, 465, 522, 531
MboI GATC 5 cut(s) 69, 110, 463, 520, 529
MboII GAAGA 1 cut(s) 535
MflI RGATCY 2 cut(s) 110, 463
MluCI AATT 3 cut(s) 144, 242, 428
MlyI GAGTC 1 cut(s) 575
MmeI TCCRAC 1 cut(s) 557
MnlI CCTC 6 cut(s) 24, 113, 393, 405, 477, 570
MseI TTAA 2 cut(s) 129, 246
MslI CAYNNNNRTG 1 cut(s) 513
MspA1I CMGCKG 2 cut(s) 19, 574
MspI CCGG 4 cut(s) 38, 108, 254, 488
MspR9I CCNGG 1 cut(s) 108
MwoI GCNNNNNNNGC 2 cut(s) 452, 580
NciI CCSGG 1 cut(s) 108
NcoI CCATGG 1 cut(s) 232
NdeII GATC 5 cut(s) 69, 110, 463, 520, 529
NlaIII CATG 4 cut(s) 37, 169, 236, 512
NlaIV GGNNCC 5 cut(s) 42, 112, 291, 300, 465
NspI RCATGY 2 cut(s) 37, 512
NspV TTCGAA 1 cut(s) 90
PaeI GCATGC 1 cut(s) 37
PcsI WCGNNNNNNNCGW 1 cut(s) 60
PfeI GAWTC 3 cut(s) 10, 103, 540
PflFI GACNNNGTC 1 cut(s) 80
PfoI TCCNGGA 1 cut(s) 106
PinAI ACCGGT 1 cut(s) 253
PkrI GCNGC 6 cut(s) 319, 478, 552, 555, 582, 604
PleI GAGTC 1 cut(s) 574
PpsI GAGTC 1 cut(s) 574
PspN4I GGNNCC 5 cut(s) 42, 112, 291, 300, 465
PsuI RGATCY 2 cut(s) 110, 463
PsyI GACNNNGTC 1 cut(s) 80
PvuII CAGCTG 2 cut(s) 19, 574
RsaI GTAC 1 cut(s) 586
RsaNI GTAC 1 cut(s) 585
RseI CAYNNNNRTG 1 cut(s) 513
SaqAI TTAA 2 cut(s) 129, 246
SatI GCNGC 6 cut(s) 318, 477, 551, 554, 581, 603
Sau3AI GATC 5 cut(s) 69, 110, 463, 520, 529
SchI GAGTC 1 cut(s) 575
ScrFI CCNGG 1 cut(s) 108
SetI ASST 7 cut(s) 21, 28, 124, 280, 288, 576, 604
SfuI TTCGAA 1 cut(s) 90
SmiMI CAYNNNNRTG 1 cut(s) 513
SphI GCATGC 1 cut(s) 37
Sse9I AATT 3 cut(s) 144, 242, 428
SsiI CCGC 3 cut(s) 163, 317, 580
StyD4I CCNGG 1 cut(s) 106
StyI CCWWGG 1 cut(s) 232
TaqI TCGA 3 cut(s) 84, 90, 96
TasI AATT 3 cut(s) 144, 242, 428
TauI GCSGC 2 cut(s) 320, 583
TfiI GAWTC 3 cut(s) 10, 103, 540
Tru1I TTAA 2 cut(s) 129, 246
Tru9I TTAA 2 cut(s) 129, 246
TscAI CASTG 1 cut(s) 208
TseI GCWGC 4 cut(s) 476, 550, 553, 602
TspRI CASTG 1 cut(s) 208
Tth111I GACNNNGTC 1 cut(s) 80
XapI RAATTY 1 cut(s) 242
XceI RCATGY 2 cut(s) 37, 512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.