MD16G1131300.v1.1

female pronucleus assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0016205
Physical Location & Seq
Reverse (-)
10029597 .. 10032382
2786 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1131300.v1.1.491

Sequence Viewer

Length: 618 bp
ATGGCTGTGAATCCTCAACTGTTCCCCAATGGCATGCCCGTTCCTTTCGTGAACGAGATGTTTGTTTTGGCCAGAGACGGCGTCGAGTTCGAGGTCGATAAGATTCCCGGATATGATGGTGGTGGTCGTCTGAAAGCAAAAGGAACAATCTACTTGTCAAATATACGGATGGTCTTTGTTTCCAACAAGCCTGTTGGGAGCTTTTTGGCTTTTGATATGCCCCTGCTTTACGTCCATGGTGAAAAGTTTAATCAACCAATTTTCCACTGCAACAACATTGCTGGTCAGGTGGAGCCAGTGGTTCCAGAAGGTCAGCATGGGGCTCTTTATTCCACTCACTCATTCAAGATTTTGTTCAAAGAAGGGGGCTGTGGGACGTTCGTTCCACTCTTTTTCAACTTGATCTCCGCAGTGAGACAATATAACCAGTATCAACACCCCAACGCTGGGTCTCAGCCTCTTGTGGATCCTTTGCAAGCAGCACAAACTCCTGTTGATGAGATGATGAGACATGCGTATGTCGATCCTAATGATCCAACGAGGATCTTCTTGCAGCAGCCCACTCCAGAGGCTCAGCTGAGGCGGCGCACATACCAGTCACAGCCCGTCGAACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

206

Amino Acids

23.02

Weight (kDa)

6.37

Isoelectric Point (pI)

44.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016205)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G11680
fragaria_vesca FvH4_4g21930
malus_domestica MD13G1135700.v1.1 MD16G1131300.v1.1
prunus_persica Prupe.1G209600_v2.0.a1
pyrus_communis pycom16g11570
rosa_chinensis RchiOBHm_Chr4g0428141
rosa_laevigata RLG00000007176
rosa_multiflora Rmu_sc0004940.1_g000010 Rmu_sc0009599.1_g000010
rosa_roxburghii Rroxscaffold_5G00369870
rosa_rugosa Rorug04G0222900
rosa_samantha Rh4BG285700 Rh4CG301000
rosa_wichuraiana Rw4G024330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 408, 583
AclWI GGATC 5 cut(s) 461, 474, 518, 527, 551
AcoI YGGCCR 1 cut(s) 69
AcyI GRCGYC 1 cut(s) 81
AfiI CCNNNNNNNGG 2 cut(s) 446, 447
AgsI TTSAA 3 cut(s) 346, 358, 397
AluBI AGCT 2 cut(s) 201, 577
AluI AGCT 2 cut(s) 201, 577
Alw26I GTCTC 4 cut(s) 69, 409, 456, 502
AlwI GGATC 5 cut(s) 461, 474, 518, 527, 551
AoxI GGCC 1 cut(s) 69
ApeKI GCWGC 3 cut(s) 479, 553, 556
AspLEI GCGC 1 cut(s) 588
AsuC2I CCSGG 1 cut(s) 108
AsuHPI GGTGA 1 cut(s) 251
BalI TGGCCA 1 cut(s) 71
BamHI GGATCC 1 cut(s) 466
BanII GRGCYC 1 cut(s) 325
BbvCI CCTCAGC 1 cut(s) 578
BbvI GCAGC 3 cut(s) 491, 565, 568
BccI CCATC 2 cut(s) 110, 163
BceAI ACGGC 1 cut(s) 94
BcnI CCSGG 1 cut(s) 108
BcoDI GTCTC 4 cut(s) 69, 409, 456, 502
BisI GCNGC 4 cut(s) 480, 554, 557, 584
BlpI GCTNAGC 1 cut(s) 573
BlsI GCNGC 4 cut(s) 481, 555, 558, 585
Bme1390I CCNGG 1 cut(s) 108
BmiI GGNNCC 3 cut(s) 294, 303, 468
BmrFI CCNGG 1 cut(s) 108
BpmI CTGGAG 1 cut(s) 549
Bpu10I CCTNAGC 1 cut(s) 578
Bpu1102I GCTNAGC 1 cut(s) 573
BpuMI CCSGG 1 cut(s) 108
BsaHI GRCGYC 1 cut(s) 81
BsaI GGTCTC 1 cut(s) 456
BsaJI CCNNGG 1 cut(s) 235
BsaXI ACNNNNNCTCC 2 cut(s) 389, 419
Bsc4I CCNNNNNNNGG 2 cut(s) 446, 447
Bse1I ACTGG 3 cut(s) 296, 427, 595
Bse3DI GCAATG 1 cut(s) 276
BseDI CCNNGG 1 cut(s) 235
BseGI GGATG 1 cut(s) 174
BseLI CCNNNNNNNGG 2 cut(s) 446, 447
BseMI GCAATG 1 cut(s) 276
BseMII CTCAG 3 cut(s) 467, 569, 587
BseNI ACTGG 3 cut(s) 296, 427, 595
BseXI GCAGC 3 cut(s) 491, 565, 568
BseYI CCCAGC 1 cut(s) 446
BshFI GGCC 1 cut(s) 71
BsiSI CCGG 1 cut(s) 108
BslFI GGGAC 1 cut(s) 388
BslI CCNNNNNNNGG 2 cut(s) 446, 447
BsmAI GTCTC 4 cut(s) 69, 409, 456, 502
BsmBI CGTCTC 1 cut(s) 69
BsmFI GGGAC 1 cut(s) 388
BsnI GGCC 1 cut(s) 71
Bso31I GGTCTC 1 cut(s) 456
Bsp1286I GDGCHC 1 cut(s) 325
Bsp143I GATC 5 cut(s) 402, 466, 523, 532, 543
Bsp1720I GCTNAGC 1 cut(s) 573
Bsp19I CCATGG 1 cut(s) 235
BspACI CCGC 2 cut(s) 408, 583
BspANI GGCC 1 cut(s) 71
BspCNI CTCAG 3 cut(s) 466, 570, 586
BspLI GGNNCC 3 cut(s) 294, 303, 468
BspPI GGATC 5 cut(s) 461, 474, 518, 527, 551
BspTNI GGTCTC 1 cut(s) 456
BsrDI GCAATG 1 cut(s) 276
BsrI ACTGG 3 cut(s) 296, 427, 595
BssECI CCNNGG 1 cut(s) 235
BssMI GATC 5 cut(s) 402, 466, 523, 532, 543
BssNI GRCGYC 1 cut(s) 81
BssT1I CCWWGG 1 cut(s) 235
Bst4CI ACNGT 1 cut(s) 21
BstACI GRCGYC 1 cut(s) 81
BstC8I GCNNGC 2 cut(s) 35, 477
BstDEI CTNAG 3 cut(s) 453, 573, 578
BstDSI CCRYGG 1 cut(s) 235
BstF5I GGATG 1 cut(s) 174
BstHHI GCGC 1 cut(s) 588
BstKTI GATC 5 cut(s) 405, 469, 526, 535, 546
BstMAI GTCTC 4 cut(s) 69, 409, 456, 502
BstMBI GATC 5 cut(s) 402, 466, 523, 532, 543
BstMWI GCNNNNNNNGC 1 cut(s) 583
BstNSI RCATGY 2 cut(s) 37, 515
BstSCI CCNGG 1 cut(s) 106
BstV1I GCAGC 3 cut(s) 491, 565, 568
BstX2I RGATCY 2 cut(s) 466, 543
BstYI RGATCY 2 cut(s) 466, 543
BsuRI GGCC 1 cut(s) 71
BtgI CCRYGG 1 cut(s) 235
BtsCI GGATG 1 cut(s) 174
BtsI GCAGTG 2 cut(s) 265, 417
BtsIMutI CAGTG 3 cut(s) 265, 303, 417
Cac8I GCNNGC 2 cut(s) 35, 477
CfoI GCGC 1 cut(s) 588
CseI GACGC 1 cut(s) 70
CviAII CATG 4 cut(s) 34, 236, 317, 512
DdeI CTNAG 3 cut(s) 453, 573, 578
DpnI GATC 5 cut(s) 404, 468, 525, 534, 545
DpnII GATC 5 cut(s) 402, 466, 523, 532, 543
EaeI YGGCCR 1 cut(s) 69
Eco130I CCWWGG 1 cut(s) 235
Eco24I GRGCYC 1 cut(s) 325
Eco31I GGTCTC 1 cut(s) 456
EcoT14I CCWWGG 1 cut(s) 235
EcoT38I GRGCYC 1 cut(s) 325
ErhI CCWWGG 1 cut(s) 235
Esp3I CGTCTC 1 cut(s) 69
FaeI CATG 4 cut(s) 37, 239, 320, 515
FaqI GGGAC 1 cut(s) 388
FatI CATG 4 cut(s) 33, 235, 316, 511
Fnu4HI GCNGC 4 cut(s) 480, 554, 557, 584
FokI GGATG 1 cut(s) 181
FriOI GRGCYC 1 cut(s) 325
Fsp4HI GCNGC 4 cut(s) 480, 554, 557, 584
GlaI GCGC 1 cut(s) 587
GluI GCNGC 4 cut(s) 480, 554, 557, 584
GsaI CCCAGC 1 cut(s) 450
GsuI CTGGAG 1 cut(s) 549
HaeIII GGCC 1 cut(s) 71
HapII CCGG 1 cut(s) 108
HgaI GACGC 1 cut(s) 70
HhaI GCGC 1 cut(s) 588
Hin1I GRCGYC 1 cut(s) 81
Hin1II CATG 4 cut(s) 37, 239, 320, 515
Hin6I GCGC 1 cut(s) 586
HinP1I GCGC 1 cut(s) 586
HinfI GANTC 2 cut(s) 10, 103
HpaII CCGG 1 cut(s) 108
HphI GGTGA 1 cut(s) 251
Hpy166II GTNNAC 1 cut(s) 52
Hpy188I TCNGA 1 cut(s) 132
Hpy188III TCNNGA 4 cut(s) 49, 305, 346, 566
Hpy8I GTNNAC 1 cut(s) 52
Hpy99I CGWCG 2 cut(s) 86, 611
HpyAV CCTTC 2 cut(s) 302, 356
HpyCH4III ACNGT 1 cut(s) 21
HpyCH4IV ACGT 2 cut(s) 231, 377
HpyCH4V TGCA 3 cut(s) 270, 475, 553
HpyF10VI GCNNNNNNNGC 1 cut(s) 583
HpyF3I CTNAG 3 cut(s) 453, 573, 578
HpySE526I ACGT 2 cut(s) 231, 377
Hsp92I GRCGYC 1 cut(s) 81
Hsp92II CATG 4 cut(s) 37, 239, 320, 515
HspAI GCGC 1 cut(s) 586
Kzo9I GATC 5 cut(s) 402, 466, 523, 532, 543
LmnI GCTCC 2 cut(s) 198, 292
Lsp1109I GCAGC 3 cut(s) 491, 565, 568
MaeII ACGT 2 cut(s) 231, 377
MaeIII GTNAC 1 cut(s) 597
MalI GATC 5 cut(s) 404, 468, 525, 534, 545
MboI GATC 5 cut(s) 402, 466, 523, 532, 543
MboII GAAGA 1 cut(s) 538
MflI RGATCY 2 cut(s) 466, 543
MhlI GDGCHC 1 cut(s) 325
MlsI TGGCCA 1 cut(s) 71
MluCI AATT 1 cut(s) 258
MluNI TGGCCA 1 cut(s) 71
MmeI TCCRAC 2 cut(s) 207, 560
MnlI CCTC 6 cut(s) 24, 85, 468, 534, 562, 573
Mox20I TGGCCA 1 cut(s) 71
MscI TGGCCA 1 cut(s) 71
MseI TTAA 2 cut(s) 249, 616
MslI CAYNNNNRTG 1 cut(s) 516
Msp20I TGGCCA 1 cut(s) 71
MspA1I CMGCKG 1 cut(s) 577
MspI CCGG 1 cut(s) 108
MspR9I CCNGG 1 cut(s) 108
MwoI GCNNNNNNNGC 1 cut(s) 583
NciI CCSGG 1 cut(s) 108
NcoI CCATGG 1 cut(s) 235
NdeII GATC 5 cut(s) 402, 466, 523, 532, 543
NlaIII CATG 4 cut(s) 37, 239, 320, 515
NlaIV GGNNCC 3 cut(s) 294, 303, 468
NmuCI GTSAC 1 cut(s) 597
NspI RCATGY 2 cut(s) 37, 515
PaeI GCATGC 1 cut(s) 37
PfeI GAWTC 2 cut(s) 10, 103
PflFI GACNNNGTC 1 cut(s) 80
PfoI TCCNGGA 1 cut(s) 106
PkrI GCNGC 4 cut(s) 481, 555, 558, 585
PspFI CCCAGC 1 cut(s) 446
PspN4I GGNNCC 3 cut(s) 294, 303, 468
PsuI RGATCY 2 cut(s) 466, 543
PsyI GACNNNGTC 1 cut(s) 80
PvuII CAGCTG 1 cut(s) 577
RseI CAYNNNNRTG 1 cut(s) 516
SaqAI TTAA 2 cut(s) 249, 616
SatI GCNGC 4 cut(s) 480, 554, 557, 584
Sau3AI GATC 5 cut(s) 402, 466, 523, 532, 543
ScrFI CCNGG 1 cut(s) 108
SduI GDGCHC 1 cut(s) 325
SetI ASST 7 cut(s) 96, 203, 234, 291, 313, 380, 579
SmiMI CAYNNNNRTG 1 cut(s) 516
SphI GCATGC 1 cut(s) 37
Sse9I AATT 1 cut(s) 258
SsiI CCGC 2 cut(s) 408, 583
StyD4I CCNGG 1 cut(s) 106
StyI CCWWGG 1 cut(s) 235
TaaI ACNGT 1 cut(s) 21
TaiI ACGT 2 cut(s) 234, 380
TaqI TCGA 5 cut(s) 84, 90, 96, 522, 609
TasI AATT 1 cut(s) 258
TauI GCSGC 1 cut(s) 586
TfiI GAWTC 2 cut(s) 10, 103
Tru1I TTAA 2 cut(s) 249, 616
Tru9I TTAA 2 cut(s) 249, 616
TscAI CASTG 3 cut(s) 272, 303, 417
TseFI GTSAC 1 cut(s) 597
TseI GCWGC 3 cut(s) 479, 553, 556
Tsp45I GTSAC 1 cut(s) 597
TspGWI ACGGA 1 cut(s) 181
TspRI CASTG 3 cut(s) 272, 303, 417
Tth111I GACNNNGTC 1 cut(s) 80
XceI RCATGY 2 cut(s) 37, 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.