MD13G1135700.v1.1

female pronucleus assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0016205
Physical Location & Seq
Reverse (-)
10441039 .. 10444085
3047 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1135700.v1.1.491

Sequence Viewer

Length: 615 bp
ATGGCTGTGAATCCTCAACTGTTCCCGAACGGCATGCCGGTTCCTTTTGTGAACGAGATGTTCGTTTTGGCTAGAGACGGCGTCGAGTTCGAGGTCGATAAGATTCCCGGATCTGATGGTGGTCGTCTGAAAGCAAAGGGAACAATCTACTTGTCAAATATAAGGATGGTCTTTGTTTCCAACAAGCCTGGTGGGAGCTTTACTGCTTTTGATATGCCCATGCTTTATGTCCATGGTGAAAAGTTTAATCAACCAATTTTCTTCTGCAACAACATTGCTGGTCAAGTGGAACCTGTGGTTCCAGAAGGTCAGCATGGGGCTCTTTATTCTACTCACTCATTCAAGATTTTGTTCAAAGAAGGGGGTTGTGGGACGTTTGTTCCACTCTTTTTCAACTTGATCTCCTCAGTTAGACAATATAATCAGTATCAACACCCCAATGCTGGGTCGCAGCCTCGTGTGGATCCTTTGCAAGCAGCAGAAACTCCTGTTGATGAGATGATGAGACACGCGTATGTCGATCCCAACGATCCAACGAGGATCTTCCTGCAGCAGCCCACTCCAGAGTCTCAGCTGAGGCGCCGTACATACCAGGCACAGCCCGCCGAACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

205

Amino Acids

22.89

Weight (kDa)

6.29

Isoelectric Point (pI)

42.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016205)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G11680
fragaria_vesca FvH4_4g21930
malus_domestica MD13G1135700.v1.1 MD16G1131300.v1.1
prunus_persica Prupe.1G209600_v2.0.a1
pyrus_communis pycom16g11570
rosa_chinensis RchiOBHm_Chr4g0428141
rosa_laevigata RLG00000007176
rosa_multiflora Rmu_sc0004940.1_g000010 Rmu_sc0009599.1_g000010
rosa_roxburghii Rroxscaffold_5G00369870
rosa_rugosa Rorug04G0222900
rosa_samantha Rh4BG285700 Rh4CG301000
rosa_wichuraiana Rw4G024330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 579
AccII CGCG 1 cut(s) 512
AciI CCGC 1 cut(s) 603
AclWI GGATC 6 cut(s) 118, 458, 471, 515, 524, 548
AcyI GRCGYC 2 cut(s) 81, 580
AfaI GTAC 1 cut(s) 586
AfiI CCNNNNNNNGG 2 cut(s) 443, 444
AflIII ACRYGT 1 cut(s) 510
AgsI TTSAA 3 cut(s) 343, 355, 394
AjnI CCWGG 2 cut(s) 187, 591
AluBI AGCT 2 cut(s) 198, 574
AluI AGCT 2 cut(s) 198, 574
Alw26I GTCTC 3 cut(s) 69, 499, 573
AlwI GGATC 6 cut(s) 118, 458, 471, 515, 524, 548
ApeKI GCWGC 4 cut(s) 451, 476, 550, 553
AspLEI GCGC 1 cut(s) 582
AsuC2I CCSGG 1 cut(s) 108
AsuHPI GGTGA 1 cut(s) 248
BamHI GGATCC 1 cut(s) 463
BanI GGYRCC 1 cut(s) 579
BanII GRGCYC 1 cut(s) 322
BauI CACGAG 1 cut(s) 456
BbvCI CCTCAGC 1 cut(s) 575
BbvI GCAGC 4 cut(s) 463, 488, 562, 565
BccI CCATC 2 cut(s) 110, 160
BceAI ACGGC 3 cut(s) 46, 94, 567
BcgI CGANNNNNNTGC 2 cut(s) 16, 50
BciT130I CCWGG 2 cut(s) 189, 593
BcnI CCSGG 1 cut(s) 108
BcoDI GTCTC 3 cut(s) 69, 499, 573
BfaI CTAG 1 cut(s) 72
BfmI CTRYAG 1 cut(s) 548
BfoI RGCGCY 1 cut(s) 583
BisI GCNGC 4 cut(s) 452, 477, 551, 554
BlsI GCNGC 4 cut(s) 453, 478, 552, 555
Bme1390I CCNGG 3 cut(s) 108, 189, 593
BmiI GGNNCC 5 cut(s) 42, 291, 300, 465, 581
BmrFI CCNGG 3 cut(s) 108, 189, 593
BpmI CTGGAG 1 cut(s) 546
Bpu10I CCTNAGC 1 cut(s) 575
BpuMI CCSGG 1 cut(s) 108
BsaHI GRCGYC 2 cut(s) 81, 580
BsaJI CCNNGG 1 cut(s) 232
BsaXI ACNNNNNCTCC 2 cut(s) 386, 416
Bsc4I CCNNNNNNNGG 2 cut(s) 443, 444
Bse118I RCCGGY 1 cut(s) 37
Bse3DI GCAATG 1 cut(s) 273
BseBI CCWGG 2 cut(s) 189, 593
BseDI CCNNGG 1 cut(s) 232
BseGI GGATG 1 cut(s) 171
BseLI CCNNNNNNNGG 2 cut(s) 443, 444
BseMI GCAATG 1 cut(s) 273
BseMII CTCAG 3 cut(s) 420, 566, 584
BseRI GAGGAG 1 cut(s) 394
BseXI GCAGC 4 cut(s) 463, 488, 562, 565
BseYI CCCAGC 1 cut(s) 443
Bsh1236I CGCG 1 cut(s) 512
BshNI GGYRCC 1 cut(s) 579
BsiSI CCGG 2 cut(s) 38, 108
BslFI GGGAC 1 cut(s) 385
BslI CCNNNNNNNGG 2 cut(s) 443, 444
BsmAI GTCTC 3 cut(s) 69, 499, 573
BsmBI CGTCTC 1 cut(s) 69
BsmFI GGGAC 1 cut(s) 385
Bsp1286I GDGCHC 1 cut(s) 322
Bsp143I GATC 6 cut(s) 110, 399, 463, 520, 529, 540
Bsp19I CCATGG 1 cut(s) 232
BspACI CCGC 1 cut(s) 603
BspCNI CTCAG 3 cut(s) 419, 567, 583
BspFNI CGCG 1 cut(s) 512
BspLI GGNNCC 5 cut(s) 42, 291, 300, 465, 581
BspMAI CTGCAG 1 cut(s) 552
BspPI GGATC 6 cut(s) 118, 458, 471, 515, 524, 548
BspT107I GGYRCC 1 cut(s) 579
BsrDI GCAATG 1 cut(s) 273
BsrFI RCCGGY 1 cut(s) 37
BssAI RCCGGY 1 cut(s) 37
BssECI CCNNGG 1 cut(s) 232
BssMI GATC 6 cut(s) 110, 399, 463, 520, 529, 540
BssNI GRCGYC 2 cut(s) 81, 580
BssSI CACGAG 1 cut(s) 456
BssT1I CCWWGG 1 cut(s) 232
Bst2BI CACGAG 1 cut(s) 456
Bst2UI CCWGG 2 cut(s) 189, 593
Bst4CI ACNGT 1 cut(s) 21
BstACI GRCGYC 2 cut(s) 81, 580
BstC8I GCNNGC 3 cut(s) 35, 474, 603
BstDEI CTNAG 3 cut(s) 406, 570, 575
BstDSI CCRYGG 1 cut(s) 232
BstF5I GGATG 1 cut(s) 171
BstFNI CGCG 1 cut(s) 512
BstH2I RGCGCY 1 cut(s) 583
BstHHI GCGC 1 cut(s) 582
BstKTI GATC 6 cut(s) 113, 402, 466, 523, 532, 543
BstMAI GTCTC 3 cut(s) 69, 499, 573
BstMBI GATC 6 cut(s) 110, 399, 463, 520, 529, 540
BstMWI GCNNNNNNNGC 1 cut(s) 602
BstNI CCWGG 2 cut(s) 189, 593
BstNSI RCATGY 1 cut(s) 37
BstSCI CCNGG 3 cut(s) 106, 187, 591
BstSFI CTRYAG 1 cut(s) 548
BstUI CGCG 1 cut(s) 512
BstV1I GCAGC 4 cut(s) 463, 488, 562, 565
BstX2I RGATCY 3 cut(s) 110, 463, 540
BstYI RGATCY 3 cut(s) 110, 463, 540
BtgI CCRYGG 1 cut(s) 232
BtsCI GGATG 1 cut(s) 171
Cac8I GCNNGC 3 cut(s) 35, 474, 603
CfoI GCGC 1 cut(s) 582
Cfr10I RCCGGY 1 cut(s) 37
CseI GACGC 1 cut(s) 70
Csp6I GTAC 1 cut(s) 585
CspCI CAANNNNNGTGG 2 cut(s) 172, 207
CviAII CATG 4 cut(s) 34, 220, 233, 314
CviJI RGCY 9 cut(s) 5, 71, 187, 198, 320, 454, 556, 574, 601
CviKI_1 RGCY 9 cut(s) 5, 71, 187, 198, 320, 454, 556, 574, 601
CviQI GTAC 1 cut(s) 585
DdeI CTNAG 3 cut(s) 406, 570, 575
DinI GGCGCC 1 cut(s) 581
DpnI GATC 6 cut(s) 112, 401, 465, 522, 531, 542
DpnII GATC 6 cut(s) 110, 399, 463, 520, 529, 540
Eco130I CCWWGG 1 cut(s) 232
Eco24I GRGCYC 1 cut(s) 322
EcoRII CCWGG 2 cut(s) 187, 591
EcoT14I CCWWGG 1 cut(s) 232
EcoT38I GRGCYC 1 cut(s) 322
EgeI GGCGCC 1 cut(s) 581
EheI GGCGCC 1 cut(s) 581
ErhI CCWWGG 1 cut(s) 232
Esp3I CGTCTC 1 cut(s) 69
FaeI CATG 4 cut(s) 37, 223, 236, 317
FaqI GGGAC 1 cut(s) 385
FatI CATG 4 cut(s) 33, 219, 232, 313
FauI CCCGC 1 cut(s) 610
Fnu4HI GCNGC 4 cut(s) 452, 477, 551, 554
FokI GGATG 1 cut(s) 178
FriOI GRGCYC 1 cut(s) 322
Fsp4HI GCNGC 4 cut(s) 452, 477, 551, 554
FspBI CTAG 1 cut(s) 72
GlaI GCGC 1 cut(s) 581
GluI GCNGC 4 cut(s) 452, 477, 551, 554
GsaI CCCAGC 1 cut(s) 447
GsuI CTGGAG 1 cut(s) 546
HaeII RGCGCY 1 cut(s) 583
HapII CCGG 2 cut(s) 38, 108
HgaI GACGC 1 cut(s) 70
HhaI GCGC 1 cut(s) 582
Hin1I GRCGYC 2 cut(s) 81, 580
Hin1II CATG 4 cut(s) 37, 223, 236, 317
Hin6I GCGC 1 cut(s) 580
HinP1I GCGC 1 cut(s) 580
HinfI GANTC 3 cut(s) 10, 103, 566
HpaII CCGG 2 cut(s) 38, 108
HphI GGTGA 1 cut(s) 248
Hpy166II GTNNAC 1 cut(s) 52
Hpy188I TCNGA 2 cut(s) 115, 129
Hpy188III TCNNGA 4 cut(s) 25, 302, 343, 563
Hpy8I GTNNAC 1 cut(s) 52
Hpy99I CGWCG 1 cut(s) 86
HpyAV CCTTC 2 cut(s) 299, 353
HpyCH4III ACNGT 1 cut(s) 21
HpyCH4IV ACGT 1 cut(s) 374
HpyCH4V TGCA 3 cut(s) 267, 472, 550
HpyF10VI GCNNNNNNNGC 1 cut(s) 602
HpyF3I CTNAG 3 cut(s) 406, 570, 575
HpySE526I ACGT 1 cut(s) 374
Hsp92I GRCGYC 2 cut(s) 81, 580
Hsp92II CATG 4 cut(s) 37, 223, 236, 317
HspAI GCGC 1 cut(s) 580
KasI GGCGCC 1 cut(s) 579
Kzo9I GATC 6 cut(s) 110, 399, 463, 520, 529, 540
LmnI GCTCC 1 cut(s) 195
Lsp1109I GCAGC 4 cut(s) 463, 488, 562, 565
MaeI CTAG 1 cut(s) 72
MaeII ACGT 1 cut(s) 374
MalI GATC 6 cut(s) 112, 401, 465, 522, 531, 542
MboI GATC 6 cut(s) 110, 399, 463, 520, 529, 540
MboII GAAGA 2 cut(s) 253, 535
MflI RGATCY 3 cut(s) 110, 463, 540
MhlI GDGCHC 1 cut(s) 322
MluCI AATT 1 cut(s) 255
MluI ACGCGT 1 cut(s) 510
Mly113I GGCGCC 1 cut(s) 580
MlyI GAGTC 1 cut(s) 575
MmeI TCCRAC 2 cut(s) 204, 557
MnlI CCTC 6 cut(s) 24, 85, 415, 465, 531, 570
MseI TTAA 2 cut(s) 246, 613
MslI CAYNNNNRTG 2 cut(s) 438, 513
MspA1I CMGCKG 1 cut(s) 574
MspI CCGG 2 cut(s) 38, 108
MspR9I CCNGG 3 cut(s) 108, 189, 593
MvaI CCWGG 2 cut(s) 189, 593
MvnI CGCG 1 cut(s) 512
MwoI GCNNNNNNNGC 1 cut(s) 602
NarI GGCGCC 1 cut(s) 580
NciI CCSGG 1 cut(s) 108
NcoI CCATGG 1 cut(s) 232
NdeII GATC 6 cut(s) 110, 399, 463, 520, 529, 540
NlaIII CATG 4 cut(s) 37, 223, 236, 317
NlaIV GGNNCC 5 cut(s) 42, 291, 300, 465, 581
NspI RCATGY 1 cut(s) 37
PaeI GCATGC 1 cut(s) 37
PcsI WCGNNNNNNNCGW 3 cut(s) 60, 516, 525
PfeI GAWTC 2 cut(s) 10, 103
PflFI GACNNNGTC 1 cut(s) 80
PfoI TCCNGGA 1 cut(s) 106
PkrI GCNGC 4 cut(s) 453, 478, 552, 555
PleI GAGTC 1 cut(s) 574
PluTI GGCGCC 1 cut(s) 583
PpsI GAGTC 1 cut(s) 574
Psp6I CCWGG 2 cut(s) 187, 591
PspFI CCCAGC 1 cut(s) 443
PspGI CCWGG 2 cut(s) 187, 591
PspN4I GGNNCC 5 cut(s) 42, 291, 300, 465, 581
PstI CTGCAG 1 cut(s) 552
PsuI RGATCY 3 cut(s) 110, 463, 540
PsyI GACNNNGTC 1 cut(s) 80
PvuII CAGCTG 1 cut(s) 574
RsaI GTAC 1 cut(s) 586
RsaNI GTAC 1 cut(s) 585
RseI CAYNNNNRTG 2 cut(s) 438, 513
SaqAI TTAA 2 cut(s) 246, 613
SatI GCNGC 4 cut(s) 452, 477, 551, 554
Sau3AI GATC 6 cut(s) 110, 399, 463, 520, 529, 540
SchI GAGTC 1 cut(s) 575
ScrFI CCNGG 3 cut(s) 108, 189, 593
SduI GDGCHC 1 cut(s) 322
SetI ASST 6 cut(s) 96, 200, 295, 310, 377, 576
SfcI CTRYAG 1 cut(s) 548
SfoI GGCGCC 1 cut(s) 581
SmiMI CAYNNNNRTG 2 cut(s) 438, 513
SphI GCATGC 1 cut(s) 37
Sse9I AATT 1 cut(s) 255
SsiI CCGC 1 cut(s) 603
SspDI GGCGCC 1 cut(s) 579
SspMI CTAG 1 cut(s) 72
StyD4I CCNGG 3 cut(s) 106, 187, 591
StyI CCWWGG 1 cut(s) 232
TaaI ACNGT 1 cut(s) 21
TaiI ACGT 1 cut(s) 377
TaqI TCGA 4 cut(s) 84, 90, 96, 519
TasI AATT 1 cut(s) 255
TfiI GAWTC 2 cut(s) 10, 103
Tru1I TTAA 2 cut(s) 246, 613
Tru9I TTAA 2 cut(s) 246, 613
TseI GCWGC 4 cut(s) 451, 476, 550, 553
Tth111I GACNNNGTC 1 cut(s) 80
XceI RCATGY 1 cut(s) 37
XspI CTAG 1 cut(s) 72
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.