Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0011670

Physical Location & Seq

Genomic Coordinates Forward (+)

25038909 .. 25043585

Gene Structure 4677 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g22100.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2808 bp

ATGGGAAGCCGTCGGGAGGATCAAGAGCTTTCCTCCGACTCTGAGGTGATGGCGAGAACCGAGAGGAAAGCTTTCAGCGGTCCTCTCGGCCCGAGTTCCTCCTCCGGGGCTTGGAGGAGGATTAAGAGCTTGGATAGGAAGAGGACTAGTACCAGCACCGAAAACGATGACGACGAGGCTTACGTCGAGATAACTCTCGACATCCTCGACGACTCGGTGGCCGTGCACAGTGTTCAGGCCGCCGGAGGTGGTGGCCACGAAGATCCTCAGCTTGCCTTGCTGGCCAAGAAGACTCTGGAAGGCAAGAAATCGGCGTCGTTCCGGTCGTCGCTCCTCCGAAGCACGTCGACGCACATACGGCAGGTCTCGCAGGAGCTGAAGCGTCTGGCTTCATTCTCGAAGCGAAACTCCGGCGCCGGCAGACGCATCGACAGGACTAGGTCCGCCACCGCGCATGCGTTGAAGAGCCTCAAGTTCATCACCGCCAAGGCCTCTGCCGCCAACGGCTGGGCCTCGGTCGAGAAACGTTTCGACGACCTCACCGCCAACTCCAATGGCCTCCTCAACTCTTCTCTCTTCGGTGAATGCATAGGAATGAACAAGGAGTCTAAGGAGTTCGCCGGCGAGCTTTTCCGGGCGATTGCTCGGAGACGCAACATTAGCGGTGATGCCATCAATAAGGCACAGCTCAGAGAGTTCTGGGAGCAGATTTCTGACGAAAGCTTCGACTCTAGGCTCCAGACTTTCTTTGACATGGTGGATAAAGACGCCGACGGAAGAATCACAGAAGAAGAAGTCAGAGAGATTATTAGTCTCAGTGCTTCTGCGAACAAACTTTCCAACATTCAGAAGCAAGCTCAGGAATATGCAGCGCTCATTATGGAAGAGCTTGACCCAGACAATGTTGGCTACATTATGGTTGAAAACTTGGAAACTTTGTTGCTGCAAGCTCCGGTGCACCACTCGGTGACGGTAAGCGACAGCCGAGTTCTGAGTCAGCTACTGAGTCAGAAGCTGAAGCCAACGCAAGAAAACAACCCAATTAAAAGATGTTACCATAAGACTAAGTACTTCTTGTTAGATAATTGGAAAAGAGTTTGGGTCTTGATGCTATGGATTAGCATAGTGTCGGCTTTGTTTGTGTACAAGTTTATTCAGTACAAGAATAAGGAAGCATTTGAAGTGATGGGGTATTGTGTTTGCGTCGCCAAAGGTGGAGCAGAGACTTTGAAGTTCAACATGGCGCTGATTTTGTTACCGGTTTGTCGAAACACGATTACTTGGCTGAGAAACAAGACCAAATTGGGGGTGGTAGTCCCGTTCGATGATAATCTGAACTTTCATAAGGTGATTGCAGTAGGAATAATCGTCGGGGTTGGATTACACGCCGGAGCACATTTGACGTGTGATTTTCCGAGGCTGATTCATGCCACGGAAGAACAGTACGAGAGCATGATACCCTACTTCGGTGAAGACCAGCCTGACAATTATTGGTGGTTTGTGAAGGGGGTGGAAGGATTGACTGGGATTGGGATGGTGGTGTTGATGACCATAGCATTCACTTTGGCCACTCCTTGGTTTAGAAGAAACAAGTTAAACTTGCCAAAGCCGCTGAAGAAACTCACAGGTTTCAATGCCTTTTGGTATTCACACCATCTATTTGTTATAGTCTACGCTCTCCTCATACTCCATGGCATTAGACTCTACCTCACTAAGAAATGGTACTACAAAACGACATGGATGTATTTGGCAGTGCCGGTTTTCCTCTACGCTTGTGAAAGGCTGATCAGGTTTTTCAGATCAAGCATCAAGCCAGTTAAGATTCTTAAGGTTGCTGTTTATCCGGGGAATGTGCTTGCATTGCACATGTCTAAGCCGCAAGGCTTCAAATACAAGAGCGGGCAGTACATGTTCGTCAACTGCGCCGCAGTTTCTCCATTCGAATGGCATCCATTTTCGATTACCTCTGCACCGGGAGATGACTACCTAAGCGTCCACATTCGAACACTCGGGGATTGGACTCGGCAGCTCAAGACCGTTTTCTCATCGGTATGTCAGCCTTCAGCTGCTGGTAAAAGCGGGTTACTCAGAGCTGACATGCAAGAAGAAAATAACCCCAGCTTTCCCAAGATCCTAATCGACGGTCCATACGGAGCACCAGCACAAGACTACAAGAAGTACGACGTCGTGTTGCTGGTCGGCCTCGGAATCGGGGCCACGCCCATGGTTAGCATTGTGAAAGACATCATCAACAACATGAATTCCTTGAAGAACAAGGAACTAGACGAGTACGCAGTTTTGGACGATGTACTGGAGAGTGGGAGGGCTGGTAATAATACTAACAACAGCAGCAGCAGTAATGGCAAGAGCAACAAGAGCAATAAAGGGTTCAGGACCCAGAAAGCTTATTACTATTGGGTGACGAGGGAACAAGGCTCGTTTGAATGGTTCAAAGGGATCCTCAATGAGGTGGCTGAGATGGATGAAAAGGGTGTGATTGAGTTGCATAACTACTGCACCAGTGTGTACGAGGAAGGTGACGCTAGGTCAGCACTGATAGCCATGCTTCAGAATCTCCACCATGCCAAGAATGGCGTAGATGTAGTCTCAGGGACGCGCGTCAAATCCCATTTCGCCAAACCCAATTGGCGCCAAGTCTACAAGAAGATCGCACTGCATCATCCTGATAGCCGAGTCGGTGTGTTTTACTGTGGGGCACCGGCACTAACAAAGGAGCTGAAGCAATTGGCGCTGGACTTTTCTCACAGGACCTCCACCAAGTTCGAGTTTCACAAAGAGAATTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005886 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0007231 GO:0008150 GO:0008152 GO:0009266 GO:0009408 GO:0009605 GO:0009607 GO:0009611 GO:0009620 GO:0009628 GO:0009987 GO:0010941 GO:0012505 GO:0016020 GO:0016174 GO:0016491 GO:0016651 GO:0023052 GO:0033500 GO:0033554 GO:0042592 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0048519 GO:0048523 GO:0048878 GO:0050664 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055114 GO:0060548 GO:0065007 GO:0065008 GO:0071214 GO:0071470 GO:0071944 GO:0072593 GO:0098542 GO:0104004

KEGG Pathways

Metabolic & Signaling

ko04016 ko04626 map04016 map04626

Pfam Domains

Protein Families

FAD_binding_8 Ferric_reduct NADPH_Ox NAD_binding_6

Protein Analysis

936

Amino Acids

105.46

Weight (kDa)

9.14

Isoelectric Point (pI)

40.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NADPH_Ox	PF08414	142 - 244	1.1e-36	Respiratory burst NADPH oxidase
EF-hand_7	PF13499	244 - 310	4e-06	EF-hand domain pair
Ferric_reduct	PF01794	414 - 561	2.5e-12	Ferric reductase like transmembrane component
FAD_binding_8	PF08022	605 - 721	1.1e-37	FAD-binding domain
NAD_binding_6	PF08030	727 - 900	7.9e-40	Ferric reductase NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011670)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G47910
fragaria_vesca	FvH4_4g22100 FvH4_4g22100
malus_domestica	MD13G1134500.v1.1 MD16G1145100.v1.1
prunus_persica	Prupe.1G211000_v2.0.a1
pyrus_communis	pycom13g11730 pycom16g11470
rosa_chinensis	RchiOBHm_Chr4g0428361
rosa_laevigata	RLG00000007160
rosa_multiflora	Rmu_co8508821.1_g000001 Rmu_sc0003384.1_g000003 Rmu_sc0010192.1_g000005
rosa_roxburghii	Rroxscaffold_5G00370020
rosa_rugosa	Rorug04G0225000
rosa_samantha	Rh4AG281800 Rh4CG303200 Rh4DG284600
rosa_wichuraiana	Rw4G024470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	2187
Acc36I	ACCTGC	1 cut(s)	352
AccB1I	GGYRCC	3 cut(s)	413, 2649, 2716
AccB7I	CCANNNNNTGG	2 cut(s)	507, 1575
AccBSI	CCGCTC	1 cut(s)	1899
AccI	GTMKAC	3 cut(s)	347, 1671, 2658
AccII	CGCG	3 cut(s)	452, 2617, 2619
AclI	AACGTT	1 cut(s)	526
AclWI	GGATC	5 cut(s)	27, 257, 2125, 2452, 2465
AcoI	YGGCCR	4 cut(s)	219, 253, 282, 1566
AcsI	RAATTY	2 cut(s)	2260, 2800
AcuI	CTGAAG	6 cut(s)	398, 1037, 1634, 2046, 2552, 2759
AcyI	GRCGYC	5 cut(s)	314, 414, 768, 2184, 2650
AdeI	CACNNNGTG	1 cut(s)	967
AfeI	AGCGCT	1 cut(s)	873
AfiI	CCNNNNNNNGG	8 cut(s)	16, 105, 111, 507, 1305, 1466, 1575, 2467
AflII	CTTAAG	1 cut(s)	1826
AflIII	ACRYGT	3 cut(s)	1403, 1866, 1908
AgeI	ACCGGT	1 cut(s)	1258
AhdI	GACNNNNNGTC	1 cut(s)	2545
AhlI	ACTAGT	1 cut(s)	146
AjiI	CACGTC	2 cut(s)	345, 1404
AleI	CACNNNNGTG	1 cut(s)	2522
AloI	GAACNNNNNNTCC	2 cut(s)	821, 853
Alw21I	GWGCWC	4 cut(s)	228, 960, 1396, 2158
Alw26I	GTCTC	5 cut(s)	370, 643, 818, 1217, 2611
Alw44I	GTGCAC	2 cut(s)	224, 956
AlwI	GGATC	5 cut(s)	27, 257, 2125, 2452, 2465
AlwNI	CAGNNNCTG	4 cut(s)	376, 1003, 1015, 2069
Ama87I	CYCGRG	2 cut(s)	91, 2009
Aor51HI	AGCGCT	1 cut(s)	873
ApaLI	GTGCAC	2 cut(s)	224, 956
ApeKI	GCWGC	6 cut(s)	869, 943, 2026, 2066, 2349, 2352
ApoI	RAATTY	2 cut(s)	2260, 2800
ArsI	GACNNNNNNTTYG	2 cut(s)	707, 739
AsiGI	ACCGGT	1 cut(s)	1258
Asp700I	GAANNNNTTC	2 cut(s)	71, 527
AspLEI	GCGC	8 cut(s)	416, 454, 874, 1246, 1925, 2619, 2652, 2752
AspS9I	GGNCC	8 cut(s)	80, 89, 441, 510, 2144, 2214, 2394, 2769
AsuC2I	CCSGG	4 cut(s)	106, 635, 1845, 1974
AsuII	TTCGAA	2 cut(s)	1941, 2002
AvaI	CYCGRG	2 cut(s)	91, 2009
AvaII	GGWCC	5 cut(s)	80, 441, 2144, 2394, 2769
BaeGI	GKGCMC	3 cut(s)	228, 960, 2719
BalI	TGGCCA	3 cut(s)	255, 284, 1568
BamHI	GGATCC	1 cut(s)	2457
BanI	GGYRCC	3 cut(s)	413, 2649, 2716
BbsI	GAAGAC	2 cut(s)	296, 1479
Bbv12I	GWGCWC	4 cut(s)	228, 960, 1396, 2158
BbvCI	CCTCAGC	1 cut(s)	267
BbvI	GCAGC	6 cut(s)	881, 930, 2038, 2053, 2361, 2364
BccI	CCATC	6 cut(s)	43, 680, 1180, 1528, 1662, 2473
BceAI	ACGGC	3 cut(s)	206, 374, 520
BcgI	CGANNNNNNTGC	2 cut(s)	409, 443
BclI	TGATCA	1 cut(s)	1785
BcnI	CCSGG	4 cut(s)	106, 635, 1845, 1974
BcoDI	GTCTC	5 cut(s)	370, 643, 818, 1217, 2611
BcuI	ACTAGT	1 cut(s)	146
BfaI	CTAG	6 cut(s)	147, 438, 732, 2282, 2544, 2806
BfoI	RGCGCY	5 cut(s)	417, 875, 1247, 2653, 2753
BfrI	CTTAAG	1 cut(s)	1826
BfuAI	ACCTGC	1 cut(s)	352
BglI	GCCNNNNNGGC	1 cut(s)	281
BmcAI	AGTACT	1 cut(s)	1070
Bme1390I	CCNGG	4 cut(s)	106, 635, 1845, 1974
Bme18I	GGWCC	5 cut(s)	80, 441, 2144, 2394, 2769
BmeRI	GACNNNNNGTC	1 cut(s)	2545
BmeT110I	CYCGRG	2 cut(s)	91, 2009
BmgBI	CACGTC	2 cut(s)	345, 1404
BmgT120I	GGNCC	8 cut(s)	80, 89, 441, 510, 2144, 2214, 2394, 2769
BmiI	GGNNCC	7 cut(s)	415, 737, 2215, 2396, 2459, 2651, 2718
BmrFI	CCNGG	4 cut(s)	106, 635, 1845, 1974
BmrI	ACTGGG	1 cut(s)	1533
BmsI	GCATC	6 cut(s)	435, 658, 1098, 1815, 1957, 2686
BmuI	ACTGGG	1 cut(s)	1533
BoxI	GACNNNNGTC	1 cut(s)	2618
BpiI	GAAGAC	2 cut(s)	296, 1479
BplI	GAGNNNNNCTC	2 cut(s)	17, 49
BpmI	CTGGAG	2 cut(s)	722, 2333
Bpu10I	CCTNAGC	3 cut(s)	267, 858, 1988
Bpu14I	TTCGAA	2 cut(s)	1941, 2002
BpuEI	CTTGAG	2 cut(s)	455, 2015
BpuMI	CCSGG	4 cut(s)	106, 635, 1845, 1974
BsaBI	GATNNNNATC	2 cut(s)	1329, 2135
BsaHI	GRCGYC	5 cut(s)	314, 414, 768, 2184, 2650
BsaI	GGTCTC	1 cut(s)	370
BsaWI	WCCGGW	3 cut(s)	321, 952, 1258
BsaXI	ACNNNNNCTCC	4 cut(s)	2314, 2344, 2756, 2786
Bsc4I	CCNNNNNNNGG	8 cut(s)	16, 105, 111, 507, 1305, 1466, 1575, 2467
Bse118I	RCCGGY	5 cut(s)	416, 620, 1258, 1756, 2719
Bse1I	ACTGG	4 cut(s)	1528, 1814, 2316, 2520
Bse3DI	GCAATG	1 cut(s)	1859
Bse8I	GATNNNNATC	2 cut(s)	1329, 2135
BseGI	GGATG	6 cut(s)	201, 1539, 1746, 1948, 2488, 2680
BseJI	GATNNNNATC	2 cut(s)	1329, 2135
BseLI	CCNNNNNNNGG	8 cut(s)	16, 105, 111, 507, 1305, 1466, 1575, 2467
BseMI	GCAATG	1 cut(s)	1859
BseNI	ACTGG	4 cut(s)	1528, 1814, 2316, 2520
BseRI	GAGGAG	5 cut(s)	91, 130, 323, 551, 1670
BseSI	GKGCMC	3 cut(s)	228, 960, 2719
BseXI	GCAGC	6 cut(s)	881, 930, 2038, 2053, 2361, 2364
BseYI	CCCAGC	2 cut(s)	507, 2117
BsgI	GTGCAG	2 cut(s)	1953, 2500
Bsh1236I	CGCG	3 cut(s)	452, 2617, 2619
Bsh1285I	CGRYCG	2 cut(s)	326, 519
BshNI	GGYRCC	3 cut(s)	413, 2649, 2716
BshTI	ACCGGT	1 cut(s)	1258
BsiEI	CGRYCG	2 cut(s)	326, 519
BsiHKAI	GWGCWC	4 cut(s)	228, 960, 1396, 2158
BsiHKCI	CYCGRG	2 cut(s)	91, 2009
BslFI	GGGAC	2 cut(s)	1301, 2626
BslI	CCNNNNNNNGG	8 cut(s)	16, 105, 111, 507, 1305, 1466, 1575, 2467
BsmAI	GTCTC	5 cut(s)	370, 643, 818, 1217, 2611
BsmBI	CGTCTC	1 cut(s)	643
BsmFI	GGGAC	2 cut(s)	1301, 2626
BsmI	GAATGC	2 cut(s)	590, 1556
Bso31I	GGTCTC	1 cut(s)	370
BsoBI	CYCGRG	2 cut(s)	91, 2009
Bsp119I	TTCGAA	2 cut(s)	1941, 2002
Bsp1286I	GDGCHC	5 cut(s)	228, 960, 1396, 2158, 2719
Bsp1407I	TGTACA	1 cut(s)	1143
Bsp143I	GATC	7 cut(s)	19, 262, 1785, 1799, 2130, 2457, 2667
Bsp19I	CCATGG	2 cut(s)	1690, 2223
BspFNI	CGCG	3 cut(s)	452, 2617, 2619
BspLI	GGNNCC	7 cut(s)	415, 737, 2215, 2396, 2459, 2651, 2718
BspMI	ACCTGC	1 cut(s)	352
BspPI	GGATC	5 cut(s)	27, 257, 2125, 2452, 2465
BspQI	GCTCTTC	2 cut(s)	458, 879
BspT104I	TTCGAA	2 cut(s)	1941, 2002
BspT107I	GGYRCC	3 cut(s)	413, 2649, 2716
BspTI	CTTAAG	1 cut(s)	1826
BspTNI	GGTCTC	1 cut(s)	370
BsrBI	CCGCTC	1 cut(s)	1899
BsrDI	GCAATG	1 cut(s)	1859
BsrFI	RCCGGY	5 cut(s)	416, 620, 1258, 1756, 2719
BsrGI	TGTACA	1 cut(s)	1143
BsrI	ACTGG	4 cut(s)	1528, 1814, 2316, 2520
BssAI	RCCGGY	5 cut(s)	416, 620, 1258, 1756, 2719
BssMI	GATC	7 cut(s)	19, 262, 1785, 1799, 2130, 2457, 2667
BssNI	GRCGYC	5 cut(s)	314, 414, 768, 2184, 2650
BssT1I	CCWWGG	4 cut(s)	486, 1574, 1690, 2223
Bst4CI	ACNGT	6 cut(s)	230, 973, 1443, 2038, 2144, 2711
Bst6I	CTCTTC	5 cut(s)	134, 458, 574, 581, 879
BstACI	GRCGYC	5 cut(s)	314, 414, 768, 2184, 2650
BstAFI	CTTAAG	1 cut(s)	1826
BstAUI	TGTACA	1 cut(s)	1143
BstBI	TTCGAA	2 cut(s)	1941, 2002
BstDSI	CCRYGG	3 cut(s)	1431, 1690, 2223
BstENI	CCTNNNNNAGG	1 cut(s)	2465
BstF5I	GGATG	6 cut(s)	201, 1539, 1746, 1948, 2488, 2680
BstFNI	CGCG	3 cut(s)	452, 2617, 2619
BstH2I	RGCGCY	5 cut(s)	417, 875, 1247, 2653, 2753
BstHHI	GCGC	8 cut(s)	416, 454, 874, 1246, 1925, 2619, 2652, 2752
BstKTI	GATC	7 cut(s)	22, 265, 1788, 1802, 2133, 2460, 2670
BstMAI	GTCTC	5 cut(s)	370, 643, 818, 1217, 2611
BstMBI	GATC	7 cut(s)	19, 262, 1785, 1799, 2130, 2457, 2667
BstMCI	CGRYCG	2 cut(s)	326, 519
BstNSI	RCATGY	4 cut(s)	458, 1870, 1912, 2101
BstPAI	GACNNNNGTC	1 cut(s)	2618
BstSCI	CCNGG	4 cut(s)	104, 633, 1843, 1972
BstSLI	GKGCMC	3 cut(s)	228, 960, 2719
BstUI	CGCG	3 cut(s)	452, 2617, 2619
BstV1I	GCAGC	6 cut(s)	881, 930, 2038, 2053, 2361, 2364
BstV2I	GAAGAC	2 cut(s)	296, 1479
BstX2I	RGATCY	3 cut(s)	262, 2130, 2457
BstXI	CCANNNNNNTGG	2 cut(s)	1944, 2224
BstYI	RGATCY	3 cut(s)	262, 2130, 2457
BtgI	CCRYGG	3 cut(s)	1431, 1690, 2223
BtrI	CACGTC	2 cut(s)	345, 1404
BtsCI	GGATG	6 cut(s)	201, 1539, 1746, 1948, 2488, 2680
BtsI	GCAGTG	2 cut(s)	1758, 2672
BtsIMutI	CAGTG	6 cut(s)	235, 823, 1758, 2527, 2552, 2672
BveI	ACCTGC	1 cut(s)	352
CaiI	CAGNNNCTG	4 cut(s)	376, 1003, 1015, 2069
CfoI	GCGC	8 cut(s)	416, 454, 874, 1246, 1925, 2619, 2652, 2752
Cfr10I	RCCGGY	5 cut(s)	416, 620, 1258, 1756, 2719
Cfr13I	GGNCC	8 cut(s)	80, 89, 441, 510, 2144, 2214, 2394, 2769
CspAI	ACCGGT	1 cut(s)	1258
DinI	GGCGCC	2 cut(s)	415, 2651
DpnI	GATC	7 cut(s)	21, 264, 1787, 1801, 2132, 2459, 2669
DpnII	GATC	7 cut(s)	19, 262, 1785, 1799, 2130, 2457, 2667
DraIII	CACNNNGTG	1 cut(s)	967
DriI	GACNNNNNGTC	1 cut(s)	2545
EaeI	YGGCCR	4 cut(s)	219, 253, 282, 1566
Eam1104I	CTCTTC	5 cut(s)	134, 458, 574, 581, 879
Eam1105I	GACNNNNNGTC	1 cut(s)	2545
EarI	CTCTTC	5 cut(s)	134, 458, 574, 581, 879
EciI	GGCGGA	1 cut(s)	433
Eco130I	CCWWGG	4 cut(s)	486, 1574, 1690, 2223
Eco147I	AGGCCT	1 cut(s)	491
Eco31I	GGTCTC	1 cut(s)	370
Eco47I	GGWCC	5 cut(s)	80, 441, 2144, 2394, 2769
Eco47III	AGCGCT	1 cut(s)	873
Eco57I	CTGAAG	6 cut(s)	398, 1037, 1634, 2046, 2552, 2759
Eco88I	CYCGRG	2 cut(s)	91, 2009
EcoNI	CCTNNNNNAGG	1 cut(s)	2465
EcoO109I	RGGNCCY	2 cut(s)	2394, 2769
EcoRI	GAATTC	1 cut(s)	2260
EcoT14I	CCWWGG	4 cut(s)	486, 1574, 1690, 2223
EcoT22I	ATGCAT	1 cut(s)	590
EgeI	GGCGCC	2 cut(s)	415, 2651
EheI	GGCGCC	2 cut(s)	415, 2651
ErhI	CCWWGG	4 cut(s)	486, 1574, 1690, 2223
Esp3I	CGTCTC	1 cut(s)	643
FalI	AAGNNNNNCTT	4 cut(s)	1058, 1090, 1583, 1615
FaqI	GGGAC	2 cut(s)	1301, 2626
FauI	CCCGC	2 cut(s)	1892, 2072
FbaI	TGATCA	1 cut(s)	1785
FblI	GTMKAC	3 cut(s)	347, 1671, 2658
FokI	GGATG	6 cut(s)	188, 1546, 1753, 1935, 2495, 2667
FspBI	CTAG	6 cut(s)	147, 438, 732, 2282, 2544, 2806
GlaI	GCGC	8 cut(s)	415, 453, 873, 1245, 1924, 2618, 2651, 2751
GsaI	CCCAGC	2 cut(s)	511, 2121
GsuI	CTGGAG	2 cut(s)	722, 2333
HaeII	RGCGCY	5 cut(s)	417, 875, 1247, 2653, 2753
HhaI	GCGC	8 cut(s)	416, 454, 874, 1246, 1925, 2619, 2652, 2752
Hin1I	GRCGYC	5 cut(s)	314, 414, 768, 2184, 2650
Hin6I	GCGC	8 cut(s)	414, 452, 872, 1244, 1923, 2617, 2650, 2750
HinP1I	GCGC	8 cut(s)	414, 452, 872, 1244, 1923, 2617, 2650, 2750
HincII	GTYRAC	2 cut(s)	348, 1918
HindII	GTYRAC	2 cut(s)	348, 1918
HindIII	AAGCTT	3 cut(s)	69, 721, 2403
Hpy166II	GTNNAC	9 cut(s)	226, 348, 958, 1144, 1672, 1918, 1996, 2527, 2659
Hpy8I	GTNNAC	9 cut(s)	226, 348, 958, 1144, 1672, 1918, 1996, 2527, 2659
HpyAV	CCTTC	5 cut(s)	293, 1498, 1508, 2070, 2528
HpyCH4III	ACNGT	6 cut(s)	230, 973, 1443, 2038, 2144, 2711
HpyCH4IV	ACGT	5 cut(s)	183, 344, 526, 1403, 2184
HpySE526I	ACGT	5 cut(s)	183, 344, 526, 1403, 2184
Hsp92I	GRCGYC	5 cut(s)	314, 414, 768, 2184, 2650
HspAI	GCGC	8 cut(s)	414, 452, 872, 1244, 1923, 2617, 2650, 2750
KasI	GGCGCC	2 cut(s)	413, 2649
KroI	GCCGGC	2 cut(s)	416, 620
KroNI	GCCGGC	2 cut(s)	418, 622
Ksp22I	TGATCA	1 cut(s)	1785
Kzo9I	GATC	7 cut(s)	19, 262, 1785, 1799, 2130, 2457, 2667
LguI	GCTCTTC	2 cut(s)	458, 879
LmnI	GCTCC	9 cut(s)	336, 373, 703, 741, 955, 1217, 1391, 2153, 2734
Lsp1109I	GCAGC	6 cut(s)	881, 930, 2038, 2053, 2361, 2364
LweI	GCATC	6 cut(s)	435, 658, 1098, 1815, 1957, 2686
MaeI	CTAG	6 cut(s)	147, 438, 732, 2282, 2544, 2806
MaeII	ACGT	5 cut(s)	183, 344, 526, 1403, 2184
MaeIII	GTNAC	6 cut(s)	967, 1052, 1254, 2082, 2419, 2537
MalI	GATC	7 cut(s)	21, 264, 1787, 1801, 2132, 2459, 2669
MbiI	CCGCTC	1 cut(s)	1899
MboI	GATC	7 cut(s)	19, 262, 1785, 1799, 2130, 2457, 2667
MfeI	CAATTG	2 cut(s)	2644, 2744
MflI	RGATCY	3 cut(s)	262, 2130, 2457
MhlI	GDGCHC	5 cut(s)	228, 960, 1396, 2158, 2719
MlsI	TGGCCA	3 cut(s)	255, 284, 1568
MluCI	AATT	8 cut(s)	1041, 1084, 1301, 1486, 2260, 2644, 2744, 2800
MluNI	TGGCCA	3 cut(s)	255, 284, 1568
Mly113I	GGCGCC	2 cut(s)	414, 2650
MmeI	TCCRAC	3 cut(s)	60, 864, 1357
Mox20I	TGGCCA	3 cut(s)	255, 284, 1568
Mph1103I	ATGCAT	1 cut(s)	590
MreI	CGCCGGCG	1 cut(s)	620
MroNI	GCCGGC	2 cut(s)	416, 620
MroXI	GAANNNNTTC	2 cut(s)	71, 527
MscI	TGGCCA	3 cut(s)	255, 284, 1568
MseI	TTAA	5 cut(s)	123, 1044, 1595, 1818, 1827
MslI	CAYNNNNRTG	5 cut(s)	593, 1942, 2050, 2222, 2522
Msp20I	TGGCCA	3 cut(s)	255, 284, 1568
MspA1I	CMGCKG	3 cut(s)	78, 1612, 2066
MspCI	CTTAAG	1 cut(s)	1826
MspR9I	CCNGG	4 cut(s)	106, 635, 1845, 1974
MunI	CAATTG	2 cut(s)	2644, 2744
Mva1269I	GAATGC	2 cut(s)	590, 1556
MvnI	CGCG	3 cut(s)	452, 2617, 2619
NaeI	GCCGGC	2 cut(s)	418, 622
NarI	GGCGCC	2 cut(s)	414, 2650
NciI	CCSGG	4 cut(s)	106, 635, 1845, 1974
NcoI	CCATGG	2 cut(s)	1690, 2223
NdeII	GATC	7 cut(s)	19, 262, 1785, 1799, 2130, 2457, 2667
NgoMIV	GCCGGC	2 cut(s)	416, 620
NlaIV	GGNNCC	7 cut(s)	415, 737, 2215, 2396, 2459, 2651, 2718
NmeAIII	GCCGAG	4 cut(s)	66, 1010, 2002, 2717
NmuCI	GTSAC	3 cut(s)	967, 2419, 2537
NsiI	ATGCAT	1 cut(s)	590
NspI	RCATGY	4 cut(s)	458, 1870, 1912, 2101
NspV	TTCGAA	2 cut(s)	1941, 2002
OliI	CACNNNNGTG	1 cut(s)	2522
PaeI	GCATGC	1 cut(s)	458
PceI	AGGCCT	1 cut(s)	491
PciI	ACATGT	2 cut(s)	1866, 1908
PciSI	GCTCTTC	2 cut(s)	458, 879
PcsI	WCGNNNNNNNCGW	5 cut(s)	171, 180, 204, 323, 723
PctI	GAATGC	2 cut(s)	590, 1556
PdiI	GCCGGC	2 cut(s)	418, 622
PdmI	GAANNNNTTC	2 cut(s)	71, 527
PfeI	GAWTC	5 cut(s)	780, 1423, 1822, 2208, 2572
PflFI	GACNNNGTC	1 cut(s)	439
PflMI	CCANNNNNTGG	2 cut(s)	507, 1575
PinAI	ACCGGT	1 cut(s)	1258
PluTI	GGCGCC	2 cut(s)	417, 2653
PpuMI	RGGWCCY	2 cut(s)	2394, 2769
PscI	ACATGT	2 cut(s)	1866, 1908
PshAI	GACNNNNGTC	1 cut(s)	2618
Psp1406I	AACGTT	1 cut(s)	526
Psp5II	RGGWCCY	2 cut(s)	2394, 2769
PspFI	CCCAGC	2 cut(s)	507, 2117
PspN4I	GGNNCC	7 cut(s)	415, 737, 2215, 2396, 2459, 2651, 2718
PspPI	GGNCC	8 cut(s)	80, 89, 441, 510, 2144, 2214, 2394, 2769
PspPPI	RGGWCCY	2 cut(s)	2394, 2769
PstNI	CAGNNNCTG	4 cut(s)	376, 1003, 1015, 2069
PsuI	RGATCY	3 cut(s)	262, 2130, 2457
PsyI	GACNNNGTC	1 cut(s)	439
PvuII	CAGCTG	1 cut(s)	2066
RseI	CAYNNNNRTG	5 cut(s)	593, 1942, 2050, 2222, 2522
SalI	GTCGAC	1 cut(s)	346
SapI	GCTCTTC	2 cut(s)	458, 879
SaqAI	TTAA	5 cut(s)	123, 1044, 1595, 1818, 1827
Sau3AI	GATC	7 cut(s)	19, 262, 1785, 1799, 2130, 2457, 2667
Sau96I	GGNCC	8 cut(s)	80, 89, 441, 510, 2144, 2214, 2394, 2769
ScaI	AGTACT	1 cut(s)	1070
ScrFI	CCNGG	4 cut(s)	106, 635, 1845, 1974
SduI	GDGCHC	5 cut(s)	228, 960, 1396, 2158, 2719
SfaNI	GCATC	6 cut(s)	435, 658, 1098, 1815, 1957, 2686
SfoI	GGCGCC	2 cut(s)	415, 2651
SfuI	TTCGAA	2 cut(s)	1941, 2002
SgrAI	CRCCGGYG	1 cut(s)	620
SgrDI	CGTCGACG	1 cut(s)	346
SinI	GGWCC	5 cut(s)	80, 441, 2144, 2394, 2769
SmiMI	CAYNNNNRTG	5 cut(s)	593, 1942, 2050, 2222, 2522
SmlI	CTYRAG	3 cut(s)	470, 1826, 2030
SmoI	CTYRAG	3 cut(s)	470, 1826, 2030
SpeI	ACTAGT	1 cut(s)	146
SphI	GCATGC	1 cut(s)	458
Sse9I	AATT	8 cut(s)	1041, 1084, 1301, 1486, 2260, 2644, 2744, 2800
SseBI	AGGCCT	1 cut(s)	491
SspDI	GGCGCC	2 cut(s)	413, 2649
SspMI	CTAG	6 cut(s)	147, 438, 732, 2282, 2544, 2806
StuI	AGGCCT	1 cut(s)	491
StyD4I	CCNGG	4 cut(s)	104, 633, 1843, 1972
StyI	CCWWGG	4 cut(s)	486, 1574, 1690, 2223
TaaI	ACNGT	6 cut(s)	230, 973, 1443, 2038, 2144, 2711
TaiI	ACGT	5 cut(s)	186, 347, 529, 1406, 2187
TaqII	GACCGA	1 cut(s)	505
TasI	AATT	8 cut(s)	1041, 1084, 1301, 1486, 2260, 2644, 2744, 2800
TatI	WGTACW	5 cut(s)	1068, 1143, 1158, 1905, 2308
TauI	GCSGC	5 cut(s)	242, 500, 1612, 1879, 1928
TfiI	GAWTC	5 cut(s)	780, 1423, 1822, 2208, 2572
Tru1I	TTAA	5 cut(s)	123, 1044, 1595, 1818, 1827
Tru9I	TTAA	5 cut(s)	123, 1044, 1595, 1818, 1827
TscAI	CASTG	6 cut(s)	235, 823, 1758, 2527, 2559, 2679
TseFI	GTSAC	3 cut(s)	967, 2419, 2537
TseI	GCWGC	6 cut(s)	869, 943, 2026, 2066, 2349, 2352
Tsp45I	GTSAC	3 cut(s)	967, 2419, 2537
TspDTI	ATGAA	7 cut(s)	381, 466, 611, 1331, 1415, 2273, 2499
TspGWI	ACGGA	3 cut(s)	789, 1448, 2166
TspRI	CASTG	6 cut(s)	235, 823, 1758, 2527, 2559, 2679
Tth111I	GACNNNGTC	1 cut(s)	439
Van91I	CCANNNNNTGG	2 cut(s)	507, 1575
Vha464I	CTTAAG	1 cut(s)	1826
VneI	GTGCAC	2 cut(s)	224, 956
VpaK11BI	GGWCC	5 cut(s)	80, 441, 2144, 2394, 2769
XagI	CCTNNNNNAGG	1 cut(s)	2465
XapI	RAATTY	2 cut(s)	2260, 2800
XceI	RCATGY	4 cut(s)	458, 1870, 1912, 2101
XcmI	CCANNNNNNNNNTGG	3 cut(s)	292, 1306, 2588
XmiI	GTMKAC	3 cut(s)	347, 1671, 2658
XmnI	GAANNNNTTC	2 cut(s)	71, 527
XspI	CTAG	6 cut(s)	147, 438, 732, 2282, 2544, 2806
ZraI	GACGTC	1 cut(s)	2185
ZrmI	AGTACT	1 cut(s)	1070
Zsp2I	ATGCAT	1 cut(s)	590

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.