Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0011670

Physical Location & Seq

Genomic Coordinates Reverse (-)

10304950 .. 10310061

Gene Structure 5112 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1134500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2889 bp

ATGAACGGTCATCACGACGAGGAAGGGATGCTTACGTCCTCGGAGTACAACTCCGACACCGAGTCGACTCAGTCCACCGCGCTCGGAGGTAGCACCCGGTTCGAAAGACGAGCTTTCAGCGGCCCGCTGGGAACGTCTAACTACGCACCGACCACGTCCTCCAACTCCAGCCGCGCGTGGCGGCGGAATAAGAGCACCAAGGTTGACAACTCCGGCGGCACGGATAAGACTACTAGTACTACTTCCGACAAAAACAACGACAATGATTACGTGGAGATCACTCTCGACATCCGAGACGACTCCGTCGCCGTCCACAGCGTCCAGGCCGCCGGCGGTGCCAGCAGCGAAGATCCCGAGCTGGCTCTGCTCGCCAAGCGGACGCTGGAGGGGAAGAAGTCGTCGTCGTTCCGGTCGTCACTTTTTCGGAACACGTCGTCGCACATCCGGCAGGTCTCTCAGGAGCTGAAGCGGCTGGCTTCCTTCACCAAGAGGCCCTCCAACGCGAAGCGGTTCGATCGGACTAAGTCAGCAACTGCCTACGCCTTGAAGAACCTCAAGTTCATCACCGCCAAGACCGGCGCCGCCTCCACCTCCGGCTGGGCCGCCGTAGACAAGCGCTACGACGAGCTCACCGCCAAATCCAACTGGCTCCTCCACTCCTCCCTCTTTGGCGAATGCATAGGGATGAACAAGGAGTCCAAGGAGTTTGCCGGAGAACTTTTCCGGGCACTTGCTCGGAGGCGGAACATAACCGGCGATATTATCAACAAGGCGCAACTGAGAGAATTCTGGGAGCAAATCTCCGATGAAAGTTTTGACTCCAGACTCCTAACATTCTTTGACATGGTGGATAGAGACGCCGATGGAAGAATCACTGAAGAAGAAGTCACAGAGATTATAAGCATGAGTGCTTCCGCAAACAAGCTCTCAAACATTCAGAAACAAGCAAAGGAATATGCAGCTTTGATCATGGAAGAACTCGACCCGGACAATGTCGGTTACATCATGGTTGAAAATTTGGAAACTTTGTTGCTGCAAGCTCCGGTTAGCCAATCTGTGAGCGTAAACGAAAGCCGGGTTTTGAGTCAGCTTCTGAGCCAGAAGCTGAAGCCGACGCAGGAGGACAACCCAATCCGAAGATGCTACCAGAAAACCAAGTACTTCGTGCTAGATAACTGGCAGAGAGTCTGGGTTATGATGCTCTGGCTTGTCATAGTTTCCGGTTTATTTGCATACAAGTTTCTGCAGTACCGCGAAAAAGAGGCGGCGTTCGAGGTGATGGGATATTGTGTCTGCATTGCCAAAGGAGGAGCAGAGATTCTCAAGTTCAACATGGCCTTGATTCTGCTTCCCGTCTGCCGAAACACAATTACTTGGCTCCGAAACAGGACCAAATTAGGCGTTGTCGTCCCGTTTGATGACAACTTAAATTTCCACAAGGTCATTGCTGTTGGAATCATAGTTGGAGTTGGACTACATGCGGGGTCGCATTTGACGTGCGATTTTCCACGGATTCTACACGCGACCGAAGATGAGTACGAGGACTTGAAACCTTACTTCGGCGAGGAGCAGCCTCCGAACTACTGGTGGTTTGTGAAGGGAGTGGAAGGGTGGACAGGTATTTTGATGGTGGTATTGATGACCATAGCCTTCACATTGGCAACTCCTTGGTTCAGAAGAAACAAACTCAATTTGCCAAAGCCTCTCAAGAAGCTCACTGGCTTCAATGCCTTCTGGTATTCCCACCACCTGTTTGTCATTGTCTATGGTCTCCTCATTGTCCACGGCATCAAGCTCTACCTTACCAAGGAGTGGTATAACAAAACGACATGGATGTATTTGGCGGTTCCGGTCACCCTCTACGCTTGCGAAAGGTTGATTAGAGCTTTTAGATCAAGCATCAAGCCAGTCAAGATTATTAAGGTTGCTGTTTATCCTGGAAATGTTCTTGCACTGCACATGTCGAAACCTCAGGGCTTCAAATACAAGAGCGGGCAGTACATGTTTGTCAACTGCGCTGCGGTTTCTCCTTTCGAATGGCACCCGTTTTCCATCACCTCAGCGCCTGGAGACGACTACCTGAGTGTACACATTCGAACACTAGGCGATTGGACGCGGCAGCTCAAAACTGTTTTCTCTGAGATTTGTCAGCCACCAACTGGTGGGAGGAGTGGCCTACTCAGAGCTGATATGCAAGGAGGAAACAATCCCAGCTTCCCTAAGATATTGATAGACGGTCCATACGGAGCACCGGCACAGGACTACAAGAAATACGACGTCGTATTGCTTGTAGGGCTCGGAATCGGAGCCACTCCCATGGTGAGCATTGTGAAGGACATCATCAACAACATGAAGATGAAGGGCAAGGAAGATGATGATTCGGAGTCCGCCCTAGAAAGCGGCAGGGCCACTCCGAATGCCTCGAGCCATAACAACAGCAACACTGGGAAGAACAAGAGCAACAAAGGGTTCAAGACCAGAAAAGCCTACTACTATTGGGTGACGAGAGAACAAGGCTCGTTTGAGTGGTTCAAAGGGATTCTGAATGAGGTGGCGGACATGGACGAGAAGGGTGTGATCGAGATGCACAACTACTGCACCAGTGTGTACGAGGAAGGGGATGCCAGGTCAGCCCTCATTGCCATGCTGCAGTCCCTCCACCACGCCAAGAACGGCGTCGACGTGGTGTCCGGCACACGCGTCAAATCCCACTTCGCCAAACCCAACTGGCGCCAAGTTTACAAGGATATCGCCCGCCACCATCCGGGCAGCCGAGTGGGGGTGTTCTACTGTGGGGCACCAGCACTAACGAAGGACCTGAAGGAGTTGGCGTTGTACTTTTCACACAAGACGACCACAAAGTTCGAGTTCCACAAAGAGAATTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005886 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0007231 GO:0008150 GO:0008152 GO:0009266 GO:0009408 GO:0009605 GO:0009607 GO:0009611 GO:0009620 GO:0009628 GO:0009987 GO:0010941 GO:0012505 GO:0016020 GO:0016174 GO:0016491 GO:0016651 GO:0023052 GO:0033500 GO:0033554 GO:0042592 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0048519 GO:0048523 GO:0048878 GO:0050664 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055114 GO:0060548 GO:0065007 GO:0065008 GO:0071214 GO:0071470 GO:0071944 GO:0072593 GO:0098542 GO:0104004

KEGG Pathways

Metabolic & Signaling

ko04016 ko04626 map04016 map04626

Pfam Domains

Protein Families

FAD_binding_8 Ferric_reduct NADPH_Ox NAD_binding_6

Protein Analysis

963

Amino Acids

108.44

Weight (kDa)

9.06

Isoelectric Point (pI)

42.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NADPH_Ox	PF08414	170 - 274	2.3e-34	Respiratory burst NADPH oxidase
Ferric_reduct	PF01794	444 - 592	1.1e-11	Ferric reductase like transmembrane component
FAD_binding_8	PF08022	636 - 752	1.5e-36	FAD-binding domain
NAD_binding_6	PF08030	758 - 944	1.7e-49	Ferric reductase NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011670)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G47910
fragaria_vesca	FvH4_4g22100 FvH4_4g22100
malus_domestica	MD13G1134500.v1.1 MD16G1145100.v1.1
prunus_persica	Prupe.1G211000_v2.0.a1
pyrus_communis	pycom13g11730 pycom16g11470
rosa_chinensis	RchiOBHm_Chr4g0428361
rosa_laevigata	RLG00000007160
rosa_multiflora	Rmu_co8508821.1_g000001 Rmu_sc0003384.1_g000003 Rmu_sc0010192.1_g000005
rosa_roxburghii	Rroxscaffold_5G00370020
rosa_rugosa	Rorug04G0225000
rosa_samantha	Rh4AG281800 Rh4CG303200 Rh4DG284600
rosa_wichuraiana	Rw4G024470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	899
AasI	GACNNNNNNGTC	1 cut(s)	302
AatII	GACGTC	1 cut(s)	2280
Acc36I	ACCTGC	1 cut(s)	439
AccB1I	GGYRCC	5 cut(s)	335, 578, 2040, 2730, 2797
AccB7I	CCANNNNNTGG	3 cut(s)	1812, 2159, 2162
AccBSI	CCGCTC	1 cut(s)	1992
AccI	GTMKAC	3 cut(s)	65, 609, 2679
AccII	CGCG	8 cut(s)	80, 174, 176, 503, 1254, 1523, 2116, 2700
AclWI	GGATC	1 cut(s)	344
AcsI	RAATTY	4 cut(s)	785, 1015, 1429, 2881
AcuI	CTGAAG	4 cut(s)	485, 897, 1127, 2840
AcyI	GRCGYC	5 cut(s)	579, 858, 2277, 2676, 2731
AfaI	GTAC	9 cut(s)	47, 238, 1160, 1250, 1538, 2000, 2088, 2609, 2837
AfeI	AGCGCT	1 cut(s)	617
AfiI	CCNNNNNNNGG	8 cut(s)	597, 1559, 1807, 1812, 2159, 2162, 2764, 2779
AflIII	ACRYGT	4 cut(s)	429, 1959, 2001, 2698
AgsI	TTSAA	8 cut(s)	547, 1013, 1330, 1549, 1726, 1981, 2473, 2533
AhdI	GACNNNNNGTC	2 cut(s)	61, 2686
AhlI	ACTAGT	1 cut(s)	233
AjiI	CACGTC	4 cut(s)	156, 432, 1497, 2683
AjnI	CCWGG	4 cut(s)	321, 1936, 2065, 2624
AleI	CACNNNNGTG	1 cut(s)	2603
AloI	GAACNNNNNNTCC	2 cut(s)	680, 712
Alw21I	GWGCWC	3 cut(s)	197, 630, 2251
Alw26I	GTCTC	5 cut(s)	288, 457, 849, 1775, 2064
AlwI	GGATC	1 cut(s)	344
AlwNI	CAGNNNCTG	5 cut(s)	463, 533, 1093, 1105, 2066
Ama87I	CYCGRG	2 cut(s)	353, 2422
Aor51HI	AGCGCT	1 cut(s)	617
AoxI	GGCC	7 cut(s)	121, 324, 491, 600, 1335, 2173, 2406
ApeKI	GCWGC	8 cut(s)	342, 959, 1033, 1570, 2018, 2119, 2647, 2769
ApoI	RAATTY	4 cut(s)	785, 1015, 1429, 2881
ArsI	GACNNNNNNTTYG	2 cut(s)	1606, 1638
Asp700I	GAANNNNTTC	1 cut(s)	509
AspLEI	GCGC	8 cut(s)	82, 176, 581, 618, 775, 2018, 2065, 2733
AspS9I	GGNCC	7 cut(s)	122, 492, 600, 1389, 2237, 2406, 2815
AsuC2I	CCSGG	5 cut(s)	97, 725, 986, 1076, 2766
AsuHPI	GGTGA	8 cut(s)	475, 556, 622, 1288, 1846, 2047, 2332, 2512
AsuII	TTCGAA	3 cut(s)	102, 2034, 2095
AvaI	CYCGRG	2 cut(s)	353, 2422
AvaII	GGWCC	3 cut(s)	1389, 2237, 2815
AxyI	CCTNAGG	1 cut(s)	1971
BaeGI	GKGCMC	2 cut(s)	730, 2800
BanI	GGYRCC	5 cut(s)	335, 578, 2040, 2730, 2797
BanII	GRGCYC	2 cut(s)	630, 2298
Bbv12I	GWGCWC	3 cut(s)	197, 630, 2251
BbvCI	CCTCAGC	1 cut(s)	2059
BbvI	GCAGC	8 cut(s)	354, 971, 1020, 1582, 2005, 2131, 2634, 2781
BccI	CCATC	5 cut(s)	857, 1273, 1621, 2060, 2769
BceAI	ACGGC	4 cut(s)	293, 590, 1801, 2689
BcgI	CGANNNNNNTGC	2 cut(s)	82, 116
BciT130I	CCWGG	4 cut(s)	323, 1938, 2067, 2626
BclI	TGATCA	1 cut(s)	966
BcnI	CCSGG	5 cut(s)	97, 725, 986, 1076, 2766
BcoDI	GTCTC	5 cut(s)	288, 457, 849, 1775, 2064
BcuI	ACTAGT	1 cut(s)	233
BfaI	CTAG	4 cut(s)	234, 1169, 2102, 2393
BfmI	CTRYAG	2 cut(s)	1244, 2648
BfoI	RGCGCY	4 cut(s)	582, 619, 2066, 2734
BfuAI	ACCTGC	1 cut(s)	439
BmcAI	AGTACT	2 cut(s)	238, 1160
Bme1390I	CCNGG	9 cut(s)	97, 323, 725, 986, 1076, 1938, 2067, 2626, 2766
Bme18I	GGWCC	3 cut(s)	1389, 2237, 2815
BmeRI	GACNNNNNGTC	2 cut(s)	61, 2686
BmeT110I	CYCGRG	2 cut(s)	353, 2422
BmgBI	CACGTC	4 cut(s)	156, 432, 1497, 2683
BmgT120I	GGNCC	7 cut(s)	122, 492, 600, 1389, 2237, 2406, 2815
BmiI	GGNNCC	9 cut(s)	337, 580, 650, 1379, 1848, 2042, 2308, 2732, 2799
BmrFI	CCNGG	9 cut(s)	97, 323, 725, 986, 1076, 1938, 2067, 2626, 2766
BmrI	ACTGGG	1 cut(s)	2454
BmsI	GCATC	7 cut(s)	18, 1130, 1188, 1797, 1908, 2574, 2611
BmuI	ACTGGG	1 cut(s)	2454
BplI	GAGNNNNNCTC	4 cut(s)	35, 67, 785, 817
BpmI	CTGGAG	4 cut(s)	151, 404, 805, 2088
Bpu10I	CCTNAGC	1 cut(s)	2059
Bpu14I	TTCGAA	3 cut(s)	102, 2034, 2095
BpuEI	CTTGAG	3 cut(s)	539, 1307, 1691
BpuMI	CCSGG	5 cut(s)	97, 725, 986, 1076, 2766
BsaAI	YACGTR	1 cut(s)	271
BsaHI	GRCGYC	5 cut(s)	579, 858, 2277, 2676, 2731
BsaI	GGTCTC	2 cut(s)	457, 1775
BsaJI	CCNNGG	8 cut(s)	39, 198, 699, 1508, 1667, 1783, 1806, 2316
BsaWI	WCCGGW	4 cut(s)	408, 1042, 1220, 1849
BsaXI	ACNNNNNCTCC	2 cut(s)	2816, 2846
Bsc4I	CCNNNNNNNGG	8 cut(s)	597, 1559, 1807, 1812, 2159, 2162, 2764, 2779
Bse118I	RCCGGY	4 cut(s)	329, 575, 752, 2251
Bse1I	ACTGG	9 cut(s)	650, 1181, 1589, 1723, 1907, 2164, 2449, 2601, 2732
Bse21I	CCTNAGG	1 cut(s)	1971
Bse3DI	GCAATG	3 cut(s)	1296, 1443, 2637
BseBI	CCWGG	4 cut(s)	323, 1938, 2067, 2626
BseDI	CCNNGG	8 cut(s)	39, 198, 699, 1508, 1667, 1783, 1806, 2316
BseGI	GGATG	7 cut(s)	33, 288, 441, 690, 1839, 2626, 2761
BseLI	CCNNNNNNNGG	8 cut(s)	597, 1559, 1807, 1812, 2159, 2162, 2764, 2779
BseMI	GCAATG	3 cut(s)	1296, 1443, 2637
BseMII	CTCAG	9 cut(s)	83, 470, 770, 1085, 1985, 2072, 2073, 2130, 2194
BseNI	ACTGG	9 cut(s)	650, 1181, 1589, 1723, 1907, 2164, 2449, 2601, 2732
BseRI	GAGGAG	6 cut(s)	641, 649, 1323, 1580, 1763, 2182
BseSI	GKGCMC	2 cut(s)	730, 2800
BseXI	GCAGC	8 cut(s)	354, 971, 1020, 1582, 2005, 2131, 2634, 2781
BseYI	CCCAGC	3 cut(s)	127, 597, 2210
BsgI	GTGCAG	2 cut(s)	1940, 2581
Bsh1236I	CGCG	8 cut(s)	80, 174, 176, 503, 1254, 1523, 2116, 2700
Bsh1285I	CGRYCG	3 cut(s)	413, 517, 1527
BshFI	GGCC	7 cut(s)	123, 326, 493, 602, 1337, 2175, 2408
BshNI	GGYRCC	5 cut(s)	335, 578, 2040, 2730, 2797
BsiEI	CGRYCG	3 cut(s)	413, 517, 1527
BsiHKAI	GWGCWC	3 cut(s)	197, 630, 2251
BsiHKCI	CYCGRG	2 cut(s)	353, 2422
BslFI	GGGAC	2 cut(s)	1394, 2638
BslI	CCNNNNNNNGG	8 cut(s)	597, 1559, 1807, 1812, 2159, 2162, 2764, 2779
BsmAI	GTCTC	5 cut(s)	288, 457, 849, 1775, 2064
BsmBI	CGTCTC	3 cut(s)	288, 849, 2064
BsmFI	GGGAC	2 cut(s)	1394, 2638
BsmI	GAATGC	2 cut(s)	680, 2422
BsnI	GGCC	7 cut(s)	123, 326, 493, 602, 1337, 2175, 2408
Bso31I	GGTCTC	2 cut(s)	457, 1775
BsoBI	CYCGRG	2 cut(s)	353, 2422
Bsp119I	TTCGAA	3 cut(s)	102, 2034, 2095
Bsp1286I	GDGCHC	6 cut(s)	197, 630, 730, 2251, 2298, 2800
Bsp1407I	TGTACA	1 cut(s)	2086
Bsp143I	GATC	6 cut(s)	276, 349, 514, 966, 1892, 2577
Bsp19I	CCATGG	1 cut(s)	2316
BspANI	GGCC	7 cut(s)	123, 326, 493, 602, 1337, 2175, 2408
BspCNI	CTCAG	9 cut(s)	82, 469, 771, 1086, 1984, 2072, 2073, 2131, 2193
BspFNI	CGCG	8 cut(s)	80, 174, 176, 503, 1254, 1523, 2116, 2700
BspLI	GGNNCC	9 cut(s)	337, 580, 650, 1379, 1848, 2042, 2308, 2732, 2799
BspMAI	CTGCAG	2 cut(s)	1248, 2652
BspMI	ACCTGC	1 cut(s)	439
BspPI	GGATC	1 cut(s)	344
BspT104I	TTCGAA	3 cut(s)	102, 2034, 2095
BspT107I	GGYRCC	5 cut(s)	335, 578, 2040, 2730, 2797
BspTNI	GGTCTC	2 cut(s)	457, 1775
BsrBI	CCGCTC	1 cut(s)	1992
BsrDI	GCAATG	3 cut(s)	1296, 1443, 2637
BsrFI	RCCGGY	4 cut(s)	329, 575, 752, 2251
BsrGI	TGTACA	1 cut(s)	2086
BsrI	ACTGG	9 cut(s)	650, 1181, 1589, 1723, 1907, 2164, 2449, 2601, 2732
BssAI	RCCGGY	4 cut(s)	329, 575, 752, 2251
BssECI	CCNNGG	8 cut(s)	39, 198, 699, 1508, 1667, 1783, 1806, 2316
BssMI	GATC	6 cut(s)	276, 349, 514, 966, 1892, 2577
BssNI	GRCGYC	5 cut(s)	579, 858, 2277, 2676, 2731
BssT1I	CCWWGG	5 cut(s)	198, 699, 1667, 1806, 2316
Bst2UI	CCWGG	4 cut(s)	323, 1938, 2067, 2626
Bst4CI	ACNGT	4 cut(s)	8, 2131, 2237, 2792
BstACI	GRCGYC	5 cut(s)	579, 858, 2277, 2676, 2731
BstAUI	TGTACA	1 cut(s)	2086
BstBAI	YACGTR	1 cut(s)	271
BstBI	TTCGAA	3 cut(s)	102, 2034, 2095
BstDSI	CCRYGG	3 cut(s)	1508, 1783, 2316
BstEII	GGTNACC	1 cut(s)	1852
BstENI	CCTNNNNNAGG	1 cut(s)	1805
BstF5I	GGATG	7 cut(s)	33, 288, 441, 690, 1839, 2626, 2761
BstFNI	CGCG	8 cut(s)	80, 174, 176, 503, 1254, 1523, 2116, 2700
BstH2I	RGCGCY	4 cut(s)	582, 619, 2066, 2734
BstHHI	GCGC	8 cut(s)	82, 176, 581, 618, 775, 2018, 2065, 2733
BstKTI	GATC	6 cut(s)	279, 352, 517, 969, 1895, 2580
BstMAI	GTCTC	5 cut(s)	288, 457, 849, 1775, 2064
BstMBI	GATC	6 cut(s)	276, 349, 514, 966, 1892, 2577
BstMCI	CGRYCG	3 cut(s)	413, 517, 1527
BstNI	CCWGG	4 cut(s)	323, 1938, 2067, 2626
BstNSI	RCATGY	3 cut(s)	1481, 1963, 2005
BstPI	GGTNACC	1 cut(s)	1852
BstSCI	CCNGG	9 cut(s)	95, 321, 723, 984, 1074, 1936, 2065, 2624, 2764
BstSFI	CTRYAG	2 cut(s)	1244, 2648
BstSLI	GKGCMC	2 cut(s)	730, 2800
BstUI	CGCG	8 cut(s)	80, 174, 176, 503, 1254, 1523, 2116, 2700
BstV1I	GCAGC	8 cut(s)	354, 971, 1020, 1582, 2005, 2131, 2634, 2781
BstX2I	RGATCY	1 cut(s)	349
BstXI	CCANNNNNNTGG	1 cut(s)	2317
BstYI	RGATCY	1 cut(s)	349
Bsu36I	CCTNAGG	1 cut(s)	1971
BsuRI	GGCC	7 cut(s)	123, 326, 493, 602, 1337, 2175, 2408
BtgI	CCRYGG	3 cut(s)	1508, 1783, 2316
BtrI	CACGTC	4 cut(s)	156, 432, 1497, 2683
BtsCI	GGATG	7 cut(s)	33, 288, 441, 690, 1839, 2626, 2761
BtsI	GCAGTG	1 cut(s)	1952
BtsIMutI	CAGTG	5 cut(s)	873, 1716, 1952, 2442, 2608
BveI	ACCTGC	1 cut(s)	439
CaiI	CAGNNNCTG	5 cut(s)	463, 533, 1093, 1105, 2066
CfoI	GCGC	8 cut(s)	82, 176, 581, 618, 775, 2018, 2065, 2733
Cfr10I	RCCGGY	4 cut(s)	329, 575, 752, 2251
Cfr13I	GGNCC	7 cut(s)	122, 492, 600, 1389, 2237, 2406, 2815
CseI	GACGC	7 cut(s)	307, 388, 866, 1123, 2122, 2665, 2689
Csp6I	GTAC	9 cut(s)	46, 237, 1159, 1249, 1537, 1999, 2087, 2608, 2836
CviQI	GTAC	9 cut(s)	46, 237, 1159, 1249, 1537, 1999, 2087, 2608, 2836
DinI	GGCGCC	2 cut(s)	580, 2732
DpnI	GATC	6 cut(s)	278, 351, 516, 968, 1894, 2579
DpnII	GATC	6 cut(s)	276, 349, 514, 966, 1892, 2577
DrdI	GACNNNNNNGTC	1 cut(s)	302
DriI	GACNNNNNGTC	2 cut(s)	61, 2686
DseDI	GACNNNNNNGTC	1 cut(s)	302
Eam1105I	GACNNNNNGTC	2 cut(s)	61, 2686
EciI	GGCGGA	4 cut(s)	199, 757, 2377, 2570
Ecl136II	GAGCTC	1 cut(s)	628
Eco130I	CCWWGG	5 cut(s)	198, 699, 1667, 1806, 2316
Eco24I	GRGCYC	2 cut(s)	630, 2298
Eco31I	GGTCTC	2 cut(s)	457, 1775
Eco32I	GATATC	1 cut(s)	2749
Eco47I	GGWCC	3 cut(s)	1389, 2237, 2815
Eco47III	AGCGCT	1 cut(s)	617
Eco53kI	GAGCTC	1 cut(s)	628
Eco57I	CTGAAG	4 cut(s)	485, 897, 1127, 2840
Eco81I	CCTNAGG	1 cut(s)	1971
Eco88I	CYCGRG	2 cut(s)	353, 2422
Eco91I	GGTNACC	1 cut(s)	1852
EcoICRI	GAGCTC	1 cut(s)	628
EcoNI	CCTNNNNNAGG	1 cut(s)	1805
EcoO109I	RGGNCCY	2 cut(s)	492, 2815
EcoO65I	GGTNACC	1 cut(s)	1852
EcoRI	GAATTC	1 cut(s)	785
EcoRII	CCWGG	4 cut(s)	321, 1936, 2065, 2624
EcoRV	GATATC	1 cut(s)	2749
EcoT14I	CCWWGG	5 cut(s)	198, 699, 1667, 1806, 2316
EcoT22I	ATGCAT	1 cut(s)	680
EcoT38I	GRGCYC	2 cut(s)	630, 2298
EgeI	GGCGCC	2 cut(s)	580, 2732
EheI	GGCGCC	2 cut(s)	580, 2732
ErhI	CCWWGG	5 cut(s)	198, 699, 1667, 1806, 2316
Esp3I	CGTCTC	3 cut(s)	288, 849, 2064
FalI	AAGNNNNNCTT	4 cut(s)	15, 47, 97, 129
FaqI	GGGAC	2 cut(s)	1394, 2638
FauI	CCCGC	4 cut(s)	132, 1474, 1985, 2762
FbaI	TGATCA	1 cut(s)	966
FblI	GTMKAC	3 cut(s)	65, 609, 2679
FokI	GGATG	7 cut(s)	40, 275, 428, 697, 1846, 2633, 2748
FriOI	GRGCYC	2 cut(s)	630, 2298
FspBI	CTAG	4 cut(s)	234, 1169, 2102, 2393
GlaI	GCGC	8 cut(s)	81, 175, 580, 617, 774, 2017, 2064, 2732
GsaI	CCCAGC	3 cut(s)	131, 601, 2214
GsuI	CTGGAG	4 cut(s)	151, 404, 805, 2088
HaeII	RGCGCY	4 cut(s)	582, 619, 2066, 2734
HaeIII	GGCC	7 cut(s)	123, 326, 493, 602, 1337, 2175, 2408
HgaI	GACGC	7 cut(s)	307, 388, 866, 1123, 2122, 2665, 2689
HhaI	GCGC	8 cut(s)	82, 176, 581, 618, 775, 2018, 2065, 2733
Hin1I	GRCGYC	5 cut(s)	579, 858, 2277, 2676, 2731
Hin6I	GCGC	8 cut(s)	80, 174, 579, 616, 773, 2016, 2063, 2731
HinP1I	GCGC	8 cut(s)	80, 174, 579, 616, 773, 2016, 2063, 2731
HincII	GTYRAC	4 cut(s)	66, 205, 2011, 2680
HindII	GTYRAC	4 cut(s)	66, 205, 2011, 2680
HphI	GGTGA	8 cut(s)	475, 556, 622, 1288, 1846, 2047, 2332, 2512
Hpy188III	TCNNGA	9 cut(s)	14, 284, 353, 458, 822, 1708, 1912, 2473, 2581
HpyCH4III	ACNGT	4 cut(s)	8, 2131, 2237, 2792
HpyCH4IV	ACGT	8 cut(s)	35, 134, 155, 270, 431, 1496, 2277, 2682
HpySE526I	ACGT	8 cut(s)	35, 134, 155, 270, 431, 1496, 2277, 2682
Hsp92I	GRCGYC	5 cut(s)	579, 858, 2277, 2676, 2731
HspAI	GCGC	8 cut(s)	80, 174, 579, 616, 773, 2016, 2063, 2731
KasI	GGCGCC	2 cut(s)	578, 2730
KroI	GCCGGC	1 cut(s)	329
KroNI	GCCGGC	1 cut(s)	331
Ksp22I	TGATCA	1 cut(s)	966
Kzo9I	GATC	6 cut(s)	276, 349, 514, 966, 1892, 2577
LmnI	GCTCC	9 cut(s)	460, 654, 793, 1045, 1310, 1383, 1567, 2246, 2306
Lsp1109I	GCAGC	8 cut(s)	354, 971, 1020, 1582, 2005, 2131, 2634, 2781
LweI	GCATC	7 cut(s)	18, 1130, 1188, 1797, 1908, 2574, 2611
MaeI	CTAG	4 cut(s)	234, 1169, 2102, 2393
MaeII	ACGT	8 cut(s)	35, 134, 155, 270, 431, 1496, 2277, 2682
MaeIII	GTNAC	5 cut(s)	414, 886, 998, 1852, 2500
MalI	GATC	6 cut(s)	278, 351, 516, 968, 1894, 2579
MbiI	CCGCTC	1 cut(s)	1992
MboI	GATC	6 cut(s)	276, 349, 514, 966, 1892, 2577
MflI	RGATCY	1 cut(s)	349
MhlI	GDGCHC	6 cut(s)	197, 630, 730, 2251, 2298, 2800
MluCI	AATT	7 cut(s)	785, 1015, 1368, 1394, 1429, 1690, 2881
MluI	ACGCGT	1 cut(s)	2698
Mly113I	GGCGCC	2 cut(s)	579, 2731
MlyI	GAGTC	9 cut(s)	61, 71, 293, 704, 812, 819, 1093, 1194, 2393
MmeI	TCCRAC	8 cut(s)	78, 186, 270, 522, 666, 1432, 1444, 1450
Mph1103I	ATGCAT	1 cut(s)	680
MreI	CGCCGGCG	1 cut(s)	329
MroNI	GCCGGC	1 cut(s)	329
MroXI	GAANNNNTTC	1 cut(s)	509
MseI	TTAA	2 cut(s)	1427, 1920
MslI	CAYNNNNRTG	5 cut(s)	683, 2315, 2354, 2603, 2642
MspA1I	CMGCKG	2 cut(s)	120, 127
MspR9I	CCNGG	9 cut(s)	97, 323, 725, 986, 1076, 1938, 2067, 2626, 2766
Mva1269I	GAATGC	2 cut(s)	680, 2422
MvaI	CCWGG	4 cut(s)	323, 1938, 2067, 2626
MvnI	CGCG	8 cut(s)	80, 174, 176, 503, 1254, 1523, 2116, 2700
NaeI	GCCGGC	1 cut(s)	331
NarI	GGCGCC	2 cut(s)	579, 2731
NciI	CCSGG	5 cut(s)	97, 725, 986, 1076, 2766
NcoI	CCATGG	1 cut(s)	2316
NdeII	GATC	6 cut(s)	276, 349, 514, 966, 1892, 2577
NgoMIV	GCCGGC	1 cut(s)	329
NlaIV	GGNNCC	9 cut(s)	337, 580, 650, 1379, 1848, 2042, 2308, 2732, 2799
NmeAIII	GCCGAG	1 cut(s)	2798
NmuCI	GTSAC	4 cut(s)	414, 886, 1852, 2500
NsiI	ATGCAT	1 cut(s)	680
NspI	RCATGY	3 cut(s)	1481, 1963, 2005
NspV	TTCGAA	3 cut(s)	102, 2034, 2095
OliI	CACNNNNGTG	1 cut(s)	2603
PaeR7I	CTCGAG	1 cut(s)	2422
PciI	ACATGT	2 cut(s)	1959, 2001
PcsI	WCGNNNNNNNCGW	4 cut(s)	12, 410, 1493, 2679
PctI	GAATGC	2 cut(s)	680, 2422
PdiI	GCCGGC	1 cut(s)	331
PdmI	GAANNNNTTC	1 cut(s)	509
PfeI	GAWTC	8 cut(s)	870, 1318, 1342, 1455, 1513, 2301, 2378, 2539
PflFI	GACNNNGTC	5 cut(s)	70, 154, 302, 523, 992
PflMI	CCANNNNNTGG	3 cut(s)	1812, 2159, 2162
PfoI	TCCNGGA	1 cut(s)	1936
Ple19I	CGATCG	1 cut(s)	517
PleI	GAGTC	9 cut(s)	61, 70, 293, 703, 812, 819, 1092, 1193, 2392
PluTI	GGCGCC	2 cut(s)	582, 2734
PpsI	GAGTC	9 cut(s)	61, 70, 293, 703, 812, 819, 1092, 1193, 2392
Ppu21I	YACGTR	1 cut(s)	271
PpuMI	RGGWCCY	1 cut(s)	2815
PscI	ACATGT	2 cut(s)	1959, 2001
PsiI	TTATAA	1 cut(s)	899
Psp124BI	GAGCTC	1 cut(s)	630
Psp5II	RGGWCCY	1 cut(s)	2815
Psp6I	CCWGG	4 cut(s)	321, 1936, 2065, 2624
PspEI	GGTNACC	1 cut(s)	1852
PspFI	CCCAGC	3 cut(s)	127, 597, 2210
PspGI	CCWGG	4 cut(s)	321, 1936, 2065, 2624
PspN4I	GGNNCC	9 cut(s)	337, 580, 650, 1379, 1848, 2042, 2308, 2732, 2799
PspPI	GGNCC	7 cut(s)	122, 492, 600, 1389, 2237, 2406, 2815
PspPPI	RGGWCCY	1 cut(s)	2815
PspXI	VCTCGAGB	1 cut(s)	2422
PstI	CTGCAG	2 cut(s)	1248, 2652
PstNI	CAGNNNCTG	5 cut(s)	463, 533, 1093, 1105, 2066
PsuI	RGATCY	1 cut(s)	349
PsyI	GACNNNGTC	5 cut(s)	70, 154, 302, 523, 992
PvuI	CGATCG	1 cut(s)	517
RsaI	GTAC	9 cut(s)	47, 238, 1160, 1250, 1538, 2000, 2088, 2609, 2837
RsaNI	GTAC	9 cut(s)	46, 237, 1159, 1249, 1537, 1999, 2087, 2608, 2836
RseI	CAYNNNNRTG	5 cut(s)	683, 2315, 2354, 2603, 2642
SacI	GAGCTC	1 cut(s)	630
SalI	GTCGAC	2 cut(s)	64, 2678
SaqAI	TTAA	2 cut(s)	1427, 1920
Sau3AI	GATC	6 cut(s)	276, 349, 514, 966, 1892, 2577
Sau96I	GGNCC	7 cut(s)	122, 492, 600, 1389, 2237, 2406, 2815
ScaI	AGTACT	2 cut(s)	238, 1160
SchI	GAGTC	9 cut(s)	61, 71, 293, 704, 812, 819, 1093, 1194, 2393
ScrFI	CCNGG	9 cut(s)	97, 323, 725, 986, 1076, 1938, 2067, 2626, 2766
SduI	GDGCHC	6 cut(s)	197, 630, 730, 2251, 2298, 2800
SfaNI	GCATC	7 cut(s)	18, 1130, 1188, 1797, 1908, 2574, 2611
SfcI	CTRYAG	2 cut(s)	1244, 2648
SfoI	GGCGCC	2 cut(s)	580, 2732
Sfr274I	CTCGAG	1 cut(s)	2422
SfuI	TTCGAA	3 cut(s)	102, 2034, 2095
SgrAI	CRCCGGYG	1 cut(s)	329
SgrDI	CGTCGACG	1 cut(s)	2678
SinI	GGWCC	3 cut(s)	1389, 2237, 2815
SlaI	CTCGAG	1 cut(s)	2422
SmiMI	CAYNNNNRTG	5 cut(s)	683, 2315, 2354, 2603, 2642
SmlI	CTYRAG	4 cut(s)	554, 1322, 1706, 2422
SmoI	CTYRAG	4 cut(s)	554, 1322, 1706, 2422
SpeI	ACTAGT	1 cut(s)	233
Sse9I	AATT	7 cut(s)	785, 1015, 1368, 1394, 1429, 1690, 2881
SspDI	GGCGCC	2 cut(s)	578, 2730
SspMI	CTAG	4 cut(s)	234, 1169, 2102, 2393
SstI	GAGCTC	1 cut(s)	630
StyD4I	CCNGG	9 cut(s)	95, 321, 723, 984, 1074, 1936, 2065, 2624, 2764
StyI	CCWWGG	5 cut(s)	198, 699, 1667, 1806, 2316
TaaI	ACNGT	4 cut(s)	8, 2131, 2237, 2792
TaiI	ACGT	8 cut(s)	38, 137, 158, 273, 434, 1499, 2280, 2685
TaqII	GACCGA	1 cut(s)	1541
TasI	AATT	7 cut(s)	785, 1015, 1368, 1394, 1429, 1690, 2881
TatI	WGTACW	6 cut(s)	45, 236, 1158, 1998, 2086, 2835
TfiI	GAWTC	8 cut(s)	870, 1318, 1342, 1455, 1513, 2301, 2378, 2539
Tru1I	TTAA	2 cut(s)	1427, 1920
Tru9I	TTAA	2 cut(s)	1427, 1920
TscAI	CASTG	5 cut(s)	880, 1723, 1959, 2449, 2608
TseFI	GTSAC	4 cut(s)	414, 886, 1852, 2500
TseI	GCWGC	8 cut(s)	342, 959, 1033, 1570, 2018, 2119, 2647, 2769
Tsp45I	GTSAC	4 cut(s)	414, 886, 1852, 2500
TspDTI	ATGAA	6 cut(s)	17, 550, 701, 822, 2366, 2372
TspGWI	ACGGA	4 cut(s)	236, 292, 1525, 2259
TspRI	CASTG	5 cut(s)	880, 1723, 1959, 2449, 2608
Tth111I	GACNNNGTC	5 cut(s)	70, 154, 302, 523, 992
Van91I	CCANNNNNTGG	3 cut(s)	1812, 2159, 2162
VpaK11BI	GGWCC	3 cut(s)	1389, 2237, 2815
XagI	CCTNNNNNAGG	1 cut(s)	1805
XapI	RAATTY	4 cut(s)	785, 1015, 1429, 2881
XceI	RCATGY	3 cut(s)	1481, 1963, 2005
XcmI	CCANNNNNNNNNTGG	1 cut(s)	379
XhoI	CTCGAG	1 cut(s)	2422
XmiI	GTMKAC	3 cut(s)	65, 609, 2679
XmnI	GAANNNNTTC	1 cut(s)	509
XspI	CTAG	4 cut(s)	234, 1169, 2102, 2393
ZraI	GACGTC	1 cut(s)	2278
ZrmI	AGTACT	2 cut(s)	238, 1160
Zsp2I	ATGCAT	1 cut(s)	680

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.