Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0011670

Physical Location & Seq

Genomic Coordinates Reverse (-)

11156971 .. 11161436

Gene Structure 4466 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1145100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2898 bp

ATGAACGGTCATCACCACGAGGAAGGGATGCTTACTTCCTCGGAGTACAACTCCGACACCGAGTCCACTCAGTCCACCGGGCCAGGGGGCAGCATCGAGTTCGAAAGACGAGCTTTCAGCGGCCCCCTGGGAACGCCAAACAGCGCCAACCACGCCCCGACCACGTCCTCCAATTCCAACCGGGCGTGGAGGCGGATTAAGAGCGCCAAGTTCGACAAGTCCGGCGGTACGGATAAGACTACTGGTAATACTTCCGACCAAAACGACGATGATACCTACGTGGAGATCACTCTCGACATCCGAGACGACTCCGTCGCCGTCCACAGCGTCCAGGCCGCCGGTGGAGCCAGTAACGAAGATCCCGAGCTGGCTCTGCTCGCCAAGCGGACACTGGAGGGGAAGAAGTCGTCATCTTTCCGGTCATCGCTTTTGCGGAACACGTCGTCGCACATCCGGCAGGTCTCTCAGGAGCTGAAGCGGCTGGCTTCCTTCAGCAAGAGGCCCTCCAACGCGAAGCGTTTCGATCGGACCAAGTCAGCAACCGCCCACGCCTTGAAGAGCCTCAAGTTCATCACCACCAAGACCGGCGCCGGCGCCACCTCCGGCTGGGCCGCCGTAGACAAGCGCTACGACGAGCTCACCGCCAAATCCAACTGGCTCCTCCCCTCTTCCCTCTTTGGCGAATGCATAGGTATGAACAAGGAGTCCAAGGAGTTTGCCGGAGAGCTTTTCCGGGCACTTGCTCGGAGGCGGAACATAACCGGCGATATCATCAACAAGGCGCAACTTAGAGAATTCTGGGAGCAAATCTCCGATGAAAGTTTTGATTCCAGACTCCAAACATTCTTTGACATGGTGGATAGAGACGCCGATGGAAGAATCACTGAAGAAGAAGTCACAGAGATTATAAGCATGAGTGCTTCTGCAAACAAGCTCTCAAACATTCAGAAACAAGCAAAGGAATATGCAGCTCTGATCATGGAAGAACTAGACCCGGACAATGTCGGCTACATCATGGTTCAAAATTTGGAAACTCTGTTGCTGCAAGCTCCGGTTGGCCAATCTGTGAGCGTAAACGAAAGCCGGGTTTTGAGTCAGCTTCTGAGTCAGAAGCTGAAGCCGACGCAGGAGAACAACCCAATCCGAAGATGCTACCAGAGAACTAAGTACTTCTTCCTTGATAACTGGCAGAGAGTCTGGGTTATGATGCTCTGGCTCGGCATAGTTTCTGGTTTGTTTGTGTACAAGTTTGTGCAGTACCGCGAAAGAGAGGAGGCATTCGAGGTGATGGGATATTGTGTCTGCATTGCCAAAGGAGGAGCTGAGACTCTCAAGTTTAACATGGCCTTGATCCTTCTTCCCGTCTGCCGGAACACAATTACTTGGCTCCGAAACAAGACCAAATTAGGCGTTGCCGTCCCGTTTGATGACAACTTAAATTTCCACAAGATCATTGCGGTTGGAATCATTGTTGGAGTTGGACTACACGTGGGGGCGCATTTGACGTGCGATTTTCCACGGATTATACATGCGACTGAAGATGAGTACGAGGACTTGAAACCTTACTTCGGCGAGGAGCAGCCTGGGAACTACTGGTGGTTCGTGAAGGGAGTGGAAGGGTGGTCAGGTATTTTGATGGTGGTATTGATGGCCATAGCCTTCACGTTGGCAACTCCTTGGTTCAGAAGAAACAAGCTCAACTTGCCAAAGCCGCTCAAGAAGCTCACAGGCTTCAACGCCTTCTGGTATTCCCACCACCTGTTTGTCATTGTCTATGCTCTCCTCATTGTCCATGGCATCAAGCTCTACCTCACCAAGGAATGGTATCATAAAACGACATGGATGTATTTGGCGGTTCCGGTCGTCCTCTACGCTTGCGAAAGGTTGATTAGAGCTTTTAGGTCAAGCATCAAGCCAGTCAAGATTCTCAAGGTTGCTGTTTATCCTGGAAATGTTCTTGCACTGCACATGTCGAAACCTCAGGGCTTCAAATACAAGAGCGGGCAGTACATGTTTGTCAACTGCGCTGCGGTTTCTCCTTTCGAATGGCACCCGTTCTCCATTACCTCATCACCAGGAGATGACTACCTAAGCGTCCACATTCGAACACTGGGCGATTGGACGCGGCAGCTCAAAACTGTTTTCTCCGAGGTATGTCAGCCACCAACTGGTGGGAAGAGCGGCCTACTCAGAGCTGATATGCAAGGAGGAAACAATCCTAGCTTCCCTAAGATACTGATAGACGGTCCATACGGAGCACCGGCACAGGACTACAAGAAATACGACGTTGTATTGCTTGTAGGGCTCGGAATCGGAGCAACTCCCATGGTGAGCATTGTGAAGGACATTATCAACAACATAAAGATGAAGAGCAGGGAAGACGATGATTCGGAGTCCGCCCTAGAAAGCGGCAGGGCCACTCCGAATGCCCCGAGCCATAACAACAGCAGCAGTGGCAAGAACAAGAGCAACAAAGGGTTCAAGACCAGAAAGGCCTACTACTATTGGGTGACGCGAGAACAAGGCTCGTTCGAGTGGTTCAAGGGGATTCTGAACGAGGTGGCGGACATGGACGAGAAGGGTGTGATCGAGATTCACAACTACTGCACCAGTGTGTACGAGGAAGGGGATGCCAGGTCAGCCCTCATTGCCATGCTCCAGTCCCTCCACCACGCCAAGAACGGCGTCGATGTGGTGTCGGGCACACGTGTCAAATCCCACTTCGCCAAGCCCAACTGGCGCCAAGTTTACAAGGATATCGCTCGCCGCCATCCGGATAGCCGAGTGGGGGTGTTTTACTGTGGGGCACCAGCACTAACAAAGGACCTCAAGGAATTGGCGTTGTACTTTTCACACAAGACCACCACGAAGTTCGACTTCCACAAAGAGAATTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005886 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0007231 GO:0008150 GO:0008152 GO:0009266 GO:0009408 GO:0009605 GO:0009607 GO:0009611 GO:0009620 GO:0009628 GO:0009987 GO:0010941 GO:0012505 GO:0016020 GO:0016174 GO:0016491 GO:0016651 GO:0023052 GO:0033500 GO:0033554 GO:0042592 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0048519 GO:0048523 GO:0048878 GO:0050664 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055114 GO:0060548 GO:0065007 GO:0065008 GO:0071214 GO:0071470 GO:0071944 GO:0072593 GO:0098542 GO:0104004

KEGG Pathways

Metabolic & Signaling

ko04016 ko04626 map04016 map04626

Pfam Domains

Protein Families

FAD_binding_8 Ferric_reduct NADPH_Ox NAD_binding_6

Protein Analysis

966

Amino Acids

108.64

Weight (kDa)

9.04

Isoelectric Point (pI)

42.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NADPH_Ox	PF08414	173 - 277	1.9e-35	Respiratory burst NADPH oxidase
EF-hand_7	PF13499	277 - 341	5.2e-06	EF-hand domain pair
Ferric_reduct	PF01794	448 - 595	1.8e-12	Ferric reductase like transmembrane component
FAD_binding_8	PF08022	639 - 755	6e-37	FAD-binding domain
NAD_binding_6	PF08030	761 - 947	1.1e-49	Ferric reductase NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0011670)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G47910
fragaria_vesca	FvH4_4g22100 FvH4_4g22100
malus_domestica	MD13G1134500.v1.1 MD16G1145100.v1.1
prunus_persica	Prupe.1G211000_v2.0.a1
pyrus_communis	pycom13g11730 pycom16g11470
rosa_chinensis	RchiOBHm_Chr4g0428361
rosa_laevigata	RLG00000007160
rosa_multiflora	Rmu_co8508821.1_g000001 Rmu_sc0003384.1_g000003 Rmu_sc0010192.1_g000005
rosa_roxburghii	Rroxscaffold_5G00370020
rosa_rugosa	Rorug04G0225000
rosa_samantha	Rh4AG281800 Rh4CG303200 Rh4DG284600
rosa_wichuraiana	Rw4G024470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	908
AasI	GACNNNNNNGTC	1 cut(s)	311
Acc36I	ACCTGC	1 cut(s)	448
AccB1I	GGYRCC	5 cut(s)	587, 593, 2049, 2739, 2806
AccB7I	CCANNNNNTGG	3 cut(s)	1821, 2168, 2171
AccBSI	CCGCTC	3 cut(s)	1714, 2001, 2181
AccI	GTMKAC	1 cut(s)	618
AccII	CGCG	4 cut(s)	512, 1263, 2125, 2515
AccIII	TCCGGA	1 cut(s)	2773
AclWI	GGATC	2 cut(s)	353, 1345
AcoI	YGGCCR	2 cut(s)	1057, 1650
AcsI	RAATTY	4 cut(s)	794, 1024, 1438, 2890
AcuI	CTGAAG	5 cut(s)	475, 494, 906, 1136, 1557
AcvI	CACGTG	2 cut(s)	1489, 2708
AcyI	GRCGYC	5 cut(s)	588, 594, 867, 2685, 2740
AfaI	GTAC	9 cut(s)	47, 229, 1169, 1244, 1259, 1547, 2009, 2618, 2846
AfeI	AGCGCT	1 cut(s)	626
AflIII	ACRYGT	6 cut(s)	438, 1486, 1968, 2010, 2705, 2707
AgsI	TTSAA	7 cut(s)	556, 1022, 1558, 1735, 1990, 2482, 2542
AhdI	GACNNNNNGTC	1 cut(s)	61
AjiI	CACGTC	3 cut(s)	165, 441, 1506
AjnI	CCWGG	7 cut(s)	82, 126, 330, 1582, 1945, 2074, 2633
AleI	CACNNNNGTG	1 cut(s)	2612
AloI	GAACNNNNNNTCC	2 cut(s)	689, 721
Alw21I	GWGCWC	2 cut(s)	639, 2260
Alw26I	GTCTC	4 cut(s)	297, 466, 858, 1319
AlwI	GGATC	2 cut(s)	353, 1345
AlwNI	CAGNNNCTG	3 cut(s)	472, 1102, 1114
Ama87I	CYCGRG	2 cut(s)	362, 2431
Aor13HI	TCCGGA	1 cut(s)	2773
Aor51HI	AGCGCT	1 cut(s)	626
ApeKI	GCWGC	7 cut(s)	90, 968, 1042, 1579, 2027, 2128, 2448
ApoI	RAATTY	4 cut(s)	794, 1024, 1438, 2890
Asp700I	GAANNNNTTC	1 cut(s)	518
AspLEI	GCGC	9 cut(s)	146, 206, 590, 596, 627, 784, 1498, 2027, 2742
AspS9I	GGNCC	8 cut(s)	80, 122, 501, 528, 609, 2246, 2415, 2824
AsuC2I	CCSGG	5 cut(s)	79, 182, 734, 995, 1085
AsuHPI	GGTGA	8 cut(s)	5, 565, 631, 1297, 1804, 2064, 2341, 2521
AsuII	TTCGAA	3 cut(s)	102, 2043, 2104
AvaI	CYCGRG	2 cut(s)	362, 2431
AvaII	GGWCC	3 cut(s)	528, 2246, 2824
AxyI	CCTNAGG	1 cut(s)	1980
BaeGI	GKGCMC	3 cut(s)	739, 2705, 2809
BaeI	ACNNNNGTAYC	2 cut(s)	264, 297
BalI	TGGCCA	2 cut(s)	1059, 1652
BanI	GGYRCC	5 cut(s)	587, 593, 2049, 2739, 2806
BanII	GRGCYC	2 cut(s)	639, 2307
BauI	CACGAG	1 cut(s)	17
BbrPI	CACGTG	2 cut(s)	1489, 2708
BbsI	GAAGAC	1 cut(s)	2385
Bbv12I	GWGCWC	2 cut(s)	639, 2260
BbvI	GCAGC	7 cut(s)	102, 980, 1029, 1591, 2014, 2140, 2460
BccI	CCATC	5 cut(s)	866, 1282, 1630, 1642, 2778
BceAI	ACGGC	4 cut(s)	302, 599, 1400, 2698
BcgI	CGANNNNNNTGC	2 cut(s)	82, 116
BciT130I	CCWGG	7 cut(s)	84, 128, 332, 1584, 1947, 2076, 2635
BclI	TGATCA	1 cut(s)	975
BcnI	CCSGG	5 cut(s)	79, 182, 734, 995, 1085
BcoDI	GTCTC	4 cut(s)	297, 466, 858, 1319
BfaI	CTAG	3 cut(s)	989, 2220, 2402
BfoI	RGCGCY	6 cut(s)	147, 207, 591, 597, 628, 2743
BfuAI	ACCTGC	1 cut(s)	448
BglI	GCCNNNNNGGC	1 cut(s)	2737
BmcAI	AGTACT	1 cut(s)	1169
Bme18I	GGWCC	3 cut(s)	528, 2246, 2824
BmeRI	GACNNNNNGTC	1 cut(s)	61
BmeT110I	CYCGRG	2 cut(s)	362, 2431
BmgBI	CACGTC	3 cut(s)	165, 441, 1506
BmgT120I	GGNCC	8 cut(s)	80, 122, 501, 528, 609, 2246, 2415, 2824
BmrI	ACTGGG	1 cut(s)	2120
BmsI	GCATC	7 cut(s)	18, 102, 1139, 1197, 1806, 1917, 2620
BmuI	ACTGGG	1 cut(s)	2120
BpiI	GAAGAC	1 cut(s)	2385
BplI	GAGNNNNNCTC	4 cut(s)	35, 67, 794, 826
BpmI	CTGGAG	2 cut(s)	413, 2642
Bpu10I	CCTNAGC	1 cut(s)	2090
Bpu14I	TTCGAA	3 cut(s)	102, 2043, 2104
BpuEI	CTTGAG	5 cut(s)	548, 1316, 1700, 1913, 2813
BpuMI	CCSGG	5 cut(s)	79, 182, 734, 995, 1085
BsaAI	YACGTR	3 cut(s)	280, 1489, 2708
BsaHI	GRCGYC	5 cut(s)	588, 594, 867, 2685, 2740
BsaI	GGTCTC	1 cut(s)	466
BsaWI	WCCGGW	4 cut(s)	417, 1051, 1858, 2773
BsaXI	ACNNNNNCTCC	2 cut(s)	2042, 2072
Bse118I	RCCGGY	5 cut(s)	338, 584, 590, 761, 2260
Bse21I	CCTNAGG	1 cut(s)	1980
Bse3DI	GCAATG	3 cut(s)	1305, 1452, 2646
BseAI	TCCGGA	1 cut(s)	2773
BseBI	CCWGG	7 cut(s)	84, 128, 332, 1584, 1947, 2076, 2635
BseGI	GGATG	6 cut(s)	33, 297, 450, 1848, 2635, 2770
BseMI	GCAATG	3 cut(s)	1305, 1452, 2646
BseMII	CTCAG	6 cut(s)	83, 479, 1094, 1314, 1994, 2203
BseRI	GAGGAG	5 cut(s)	650, 1286, 1332, 1589, 1772
BseSI	GKGCMC	3 cut(s)	739, 2705, 2809
BseXI	GCAGC	7 cut(s)	102, 980, 1029, 1591, 2014, 2140, 2460
BseYI	CCCAGC	1 cut(s)	606
BsgI	GTGCAG	3 cut(s)	1274, 1949, 2590
Bsh1236I	CGCG	4 cut(s)	512, 1263, 2125, 2515
Bsh1285I	CGRYCG	2 cut(s)	526, 1863
BshNI	GGYRCC	5 cut(s)	587, 593, 2049, 2739, 2806
BsiEI	CGRYCG	2 cut(s)	526, 1863
BsiHKAI	GWGCWC	2 cut(s)	639, 2260
BsiHKCI	CYCGRG	2 cut(s)	362, 2431
BslFI	GGGAC	2 cut(s)	1403, 2647
BsmAI	GTCTC	4 cut(s)	297, 466, 858, 1319
BsmBI	CGTCTC	2 cut(s)	297, 858
BsmFI	GGGAC	2 cut(s)	1403, 2647
BsmI	GAATGC	3 cut(s)	689, 1277, 2431
Bso31I	GGTCTC	1 cut(s)	466
BsoBI	CYCGRG	2 cut(s)	362, 2431
Bsp119I	TTCGAA	3 cut(s)	102, 2043, 2104
Bsp1286I	GDGCHC	6 cut(s)	639, 739, 2260, 2307, 2705, 2809
Bsp13I	TCCGGA	1 cut(s)	2773
Bsp1407I	TGTACA	1 cut(s)	1242
Bsp143I	GATC	7 cut(s)	285, 358, 523, 975, 1350, 1449, 2586
Bsp19I	CCATGG	2 cut(s)	1792, 2325
BspCNI	CTCAG	6 cut(s)	82, 478, 1095, 1315, 1993, 2202
BspEI	TCCGGA	1 cut(s)	2773
BspFNI	CGCG	4 cut(s)	512, 1263, 2125, 2515
BspMI	ACCTGC	1 cut(s)	448
BspPI	GGATC	2 cut(s)	353, 1345
BspQI	GCTCTTC	3 cut(s)	551, 2171, 2363
BspT104I	TTCGAA	3 cut(s)	102, 2043, 2104
BspT107I	GGYRCC	5 cut(s)	587, 593, 2049, 2739, 2806
BspTNI	GGTCTC	1 cut(s)	466
BsrBI	CCGCTC	3 cut(s)	1714, 2001, 2181
BsrDI	GCAATG	3 cut(s)	1305, 1452, 2646
BsrFI	RCCGGY	5 cut(s)	338, 584, 590, 761, 2260
BsrGI	TGTACA	1 cut(s)	1242
BssAI	RCCGGY	5 cut(s)	338, 584, 590, 761, 2260
BssMI	GATC	7 cut(s)	285, 358, 523, 975, 1350, 1449, 2586
BssNI	GRCGYC	5 cut(s)	588, 594, 867, 2685, 2740
BssSI	CACGAG	1 cut(s)	17
BssT1I	CCWWGG	5 cut(s)	708, 1676, 1792, 1815, 2325
Bst2BI	CACGAG	1 cut(s)	17
Bst2UI	CCWGG	7 cut(s)	84, 128, 332, 1584, 1947, 2076, 2635
Bst4CI	ACNGT	4 cut(s)	8, 2140, 2246, 2801
Bst6I	CTCTTC	4 cut(s)	551, 673, 2171, 2363
BstACI	GRCGYC	5 cut(s)	588, 594, 867, 2685, 2740
BstAUI	TGTACA	1 cut(s)	1242
BstBAI	YACGTR	3 cut(s)	280, 1489, 2708
BstBI	TTCGAA	3 cut(s)	102, 2043, 2104
BstC8I	GCNNGC	8 cut(s)	369, 378, 483, 592, 1047, 1876, 2003, 2764
BstDSI	CCRYGG	3 cut(s)	1517, 1792, 2325
BstENI	CCTNNNNNAGG	1 cut(s)	1814
BstF5I	GGATG	6 cut(s)	33, 297, 450, 1848, 2635, 2770
BstFNI	CGCG	4 cut(s)	512, 1263, 2125, 2515
BstH2I	RGCGCY	6 cut(s)	147, 207, 591, 597, 628, 2743
BstHHI	GCGC	9 cut(s)	146, 206, 590, 596, 627, 784, 1498, 2027, 2742
BstKTI	GATC	7 cut(s)	288, 361, 526, 978, 1353, 1452, 2589
BstMAI	GTCTC	4 cut(s)	297, 466, 858, 1319
BstMBI	GATC	7 cut(s)	285, 358, 523, 975, 1350, 1449, 2586
BstMCI	CGRYCG	2 cut(s)	526, 1863
BstNI	CCWGG	7 cut(s)	84, 128, 332, 1584, 1947, 2076, 2635
BstNSI	RCATGY	3 cut(s)	1532, 1972, 2014
BstSLI	GKGCMC	3 cut(s)	739, 2705, 2809
BstUI	CGCG	4 cut(s)	512, 1263, 2125, 2515
BstV1I	GCAGC	7 cut(s)	102, 980, 1029, 1591, 2014, 2140, 2460
BstV2I	GAAGAC	1 cut(s)	2385
BstX2I	RGATCY	1 cut(s)	358
BstYI	RGATCY	1 cut(s)	358
Bsu36I	CCTNAGG	1 cut(s)	1980
BtgI	CCRYGG	3 cut(s)	1517, 1792, 2325
BtgZI	GCGATG	1 cut(s)	408
BtrI	CACGTC	3 cut(s)	165, 441, 1506
BtsCI	GGATG	6 cut(s)	33, 297, 450, 1848, 2635, 2770
BtsI	GCAGTG	2 cut(s)	1961, 2458
BtsIMutI	CAGTG	6 cut(s)	389, 882, 1961, 2108, 2458, 2617
BveI	ACCTGC	1 cut(s)	448
Cac8I	GCNNGC	8 cut(s)	369, 378, 483, 592, 1047, 1876, 2003, 2764
CaiI	CAGNNNCTG	3 cut(s)	472, 1102, 1114
CfoI	GCGC	9 cut(s)	146, 206, 590, 596, 627, 784, 1498, 2027, 2742
Cfr10I	RCCGGY	5 cut(s)	338, 584, 590, 761, 2260
Cfr13I	GGNCC	8 cut(s)	80, 122, 501, 528, 609, 2246, 2415, 2824
CseI	GACGC	7 cut(s)	316, 875, 1132, 2083, 2131, 2521, 2674
Csp6I	GTAC	9 cut(s)	46, 228, 1168, 1243, 1258, 1546, 2008, 2617, 2845
CviQI	GTAC	9 cut(s)	46, 228, 1168, 1243, 1258, 1546, 2008, 2617, 2845
DinI	GGCGCC	3 cut(s)	589, 595, 2741
DpnI	GATC	7 cut(s)	287, 360, 525, 977, 1352, 1451, 2588
DpnII	GATC	7 cut(s)	285, 358, 523, 975, 1350, 1449, 2586
DrdI	GACNNNNNNGTC	1 cut(s)	311
DriI	GACNNNNNGTC	1 cut(s)	61
DseDI	GACNNNNNNGTC	1 cut(s)	311
EaeI	YGGCCR	2 cut(s)	1057, 1650
Eam1104I	CTCTTC	4 cut(s)	551, 673, 2171, 2363
Eam1105I	GACNNNNNGTC	1 cut(s)	61
EarI	CTCTTC	4 cut(s)	551, 673, 2171, 2363
EciI	GGCGGA	4 cut(s)	208, 766, 2386, 2579
Ecl136II	GAGCTC	1 cut(s)	637
Eco130I	CCWWGG	5 cut(s)	708, 1676, 1792, 1815, 2325
Eco147I	AGGCCT	1 cut(s)	2495
Eco24I	GRGCYC	2 cut(s)	639, 2307
Eco31I	GGTCTC	1 cut(s)	466
Eco32I	GATATC	2 cut(s)	769, 2758
Eco47I	GGWCC	3 cut(s)	528, 2246, 2824
Eco47III	AGCGCT	1 cut(s)	626
Eco53kI	GAGCTC	1 cut(s)	637
Eco57I	CTGAAG	5 cut(s)	475, 494, 906, 1136, 1557
Eco72I	CACGTG	2 cut(s)	1489, 2708
Eco81I	CCTNAGG	1 cut(s)	1980
Eco88I	CYCGRG	2 cut(s)	362, 2431
EcoICRI	GAGCTC	1 cut(s)	637
EcoNI	CCTNNNNNAGG	1 cut(s)	1814
EcoO109I	RGGNCCY	2 cut(s)	501, 2824
EcoRI	GAATTC	1 cut(s)	794
EcoRII	CCWGG	7 cut(s)	82, 126, 330, 1582, 1945, 2074, 2633
EcoRV	GATATC	2 cut(s)	769, 2758
EcoT14I	CCWWGG	5 cut(s)	708, 1676, 1792, 1815, 2325
EcoT22I	ATGCAT	1 cut(s)	689
EcoT38I	GRGCYC	2 cut(s)	639, 2307
EgeI	GGCGCC	3 cut(s)	589, 595, 2741
EheI	GGCGCC	3 cut(s)	589, 595, 2741
ErhI	CCWWGG	5 cut(s)	708, 1676, 1792, 1815, 2325
Esp3I	CGTCTC	2 cut(s)	297, 858
FaqI	GGGAC	2 cut(s)	1403, 2647
FauI	CCCGC	1 cut(s)	1994
FbaI	TGATCA	1 cut(s)	975
FblI	GTMKAC	1 cut(s)	618
FokI	GGATG	6 cut(s)	40, 284, 437, 1855, 2642, 2757
FriOI	GRGCYC	2 cut(s)	639, 2307
FspBI	CTAG	3 cut(s)	989, 2220, 2402
GlaI	GCGC	9 cut(s)	145, 205, 589, 595, 626, 783, 1497, 2026, 2741
GsaI	CCCAGC	1 cut(s)	610
GsuI	CTGGAG	2 cut(s)	413, 2642
HaeII	RGCGCY	6 cut(s)	147, 207, 591, 597, 628, 2743
HgaI	GACGC	7 cut(s)	316, 875, 1132, 2083, 2131, 2521, 2674
HhaI	GCGC	9 cut(s)	146, 206, 590, 596, 627, 784, 1498, 2027, 2742
Hin1I	GRCGYC	5 cut(s)	588, 594, 867, 2685, 2740
Hin6I	GCGC	9 cut(s)	144, 204, 588, 594, 625, 782, 1496, 2025, 2740
HinP1I	GCGC	9 cut(s)	144, 204, 588, 594, 625, 782, 1496, 2025, 2740
HincII	GTYRAC	1 cut(s)	2020
HindII	GTYRAC	1 cut(s)	2020
HphI	GGTGA	8 cut(s)	5, 565, 631, 1297, 1804, 2064, 2341, 2521
Hpy99I	CGWCG	8 cut(s)	269, 317, 445, 448, 635, 1126, 2288, 2690
HpyCH4III	ACNGT	4 cut(s)	8, 2140, 2246, 2801
HpyCH4IV	ACGT	8 cut(s)	164, 279, 440, 1488, 1505, 1664, 2286, 2707
HpySE526I	ACGT	8 cut(s)	164, 279, 440, 1488, 1505, 1664, 2286, 2707
Hsp92I	GRCGYC	5 cut(s)	588, 594, 867, 2685, 2740
HspAI	GCGC	9 cut(s)	144, 204, 588, 594, 625, 782, 1496, 2025, 2740
KasI	GGCGCC	3 cut(s)	587, 593, 2739
Kpn2I	TCCGGA	1 cut(s)	2773
KroI	GCCGGC	1 cut(s)	590
KroNI	GCCGGC	1 cut(s)	592
Ksp22I	TGATCA	1 cut(s)	975
Kzo9I	GATC	7 cut(s)	285, 358, 523, 975, 1350, 1449, 2586
LguI	GCTCTTC	3 cut(s)	551, 2171, 2363
Lsp1109I	GCAGC	7 cut(s)	102, 980, 1029, 1591, 2014, 2140, 2460
LweI	GCATC	7 cut(s)	18, 102, 1139, 1197, 1806, 1917, 2620
MaeI	CTAG	3 cut(s)	989, 2220, 2402
MaeII	ACGT	8 cut(s)	164, 279, 440, 1488, 1505, 1664, 2286, 2707
MaeIII	GTNAC	3 cut(s)	350, 895, 2509
MalI	GATC	7 cut(s)	287, 360, 525, 977, 1352, 1451, 2588
MbiI	CCGCTC	3 cut(s)	1714, 2001, 2181
MboI	GATC	7 cut(s)	285, 358, 523, 975, 1350, 1449, 2586
MflI	RGATCY	1 cut(s)	358
MhlI	GDGCHC	6 cut(s)	639, 739, 2260, 2307, 2705, 2809
MlsI	TGGCCA	2 cut(s)	1059, 1652
MluCI	AATT	8 cut(s)	172, 794, 1024, 1377, 1403, 1438, 2834, 2890
MluNI	TGGCCA	2 cut(s)	1059, 1652
Mly113I	GGCGCC	3 cut(s)	588, 594, 2740
MlyI	GAGTC	9 cut(s)	71, 302, 713, 828, 1102, 1114, 1203, 1321, 2402
MmeI	TCCRAC	8 cut(s)	78, 201, 279, 531, 675, 1441, 1453, 1459
Mox20I	TGGCCA	2 cut(s)	1059, 1652
Mph1103I	ATGCAT	1 cut(s)	689
MreI	CGCCGGCG	1 cut(s)	590
MroI	TCCGGA	1 cut(s)	2773
MroNI	GCCGGC	1 cut(s)	590
MroXI	GAANNNNTTC	1 cut(s)	518
MscI	TGGCCA	2 cut(s)	1059, 1652
MseI	TTAA	3 cut(s)	198, 1338, 1436
MslI	CAYNNNNRTG	4 cut(s)	692, 2363, 2612, 2651
Msp20I	TGGCCA	2 cut(s)	1059, 1652
MspA1I	CMGCKG	1 cut(s)	120
Mva1269I	GAATGC	3 cut(s)	689, 1277, 2431
MvaI	CCWGG	7 cut(s)	84, 128, 332, 1584, 1947, 2076, 2635
MvnI	CGCG	4 cut(s)	512, 1263, 2125, 2515
NaeI	GCCGGC	1 cut(s)	592
NarI	GGCGCC	3 cut(s)	588, 594, 2740
NciI	CCSGG	5 cut(s)	79, 182, 734, 995, 1085
NcoI	CCATGG	2 cut(s)	1792, 2325
NdeII	GATC	7 cut(s)	285, 358, 523, 975, 1350, 1449, 2586
NgoMIV	GCCGGC	1 cut(s)	590
NmeAIII	GCCGAG	2 cut(s)	1197, 2807
NmuCI	GTSAC	2 cut(s)	895, 2509
NsiI	ATGCAT	1 cut(s)	689
NspI	RCATGY	3 cut(s)	1532, 1972, 2014
NspV	TTCGAA	3 cut(s)	102, 2043, 2104
OliI	CACNNNNGTG	1 cut(s)	2612
PasI	CCCWGGG	1 cut(s)	127
PceI	AGGCCT	1 cut(s)	2495
PciI	ACATGT	2 cut(s)	1968, 2010
PciSI	GCTCTTC	3 cut(s)	551, 2171, 2363
PcsI	WCGNNNNNNNCGW	1 cut(s)	360
PctI	GAATGC	3 cut(s)	689, 1277, 2431
PdiI	GCCGGC	1 cut(s)	592
PdmI	GAANNNNTTC	1 cut(s)	518
PfeI	GAWTC	8 cut(s)	827, 879, 1464, 1924, 2310, 2387, 2548, 2593
PflFI	GACNNNGTC	4 cut(s)	163, 311, 532, 1001
PflMI	CCANNNNNTGG	3 cut(s)	1821, 2168, 2171
PfoI	TCCNGGA	1 cut(s)	1945
Ple19I	CGATCG	1 cut(s)	526
PleI	GAGTC	9 cut(s)	70, 302, 712, 828, 1101, 1113, 1202, 1321, 2401
PluTI	GGCGCC	3 cut(s)	591, 597, 2743
PmaCI	CACGTG	2 cut(s)	1489, 2708
PmlI	CACGTG	2 cut(s)	1489, 2708
PpsI	GAGTC	9 cut(s)	70, 302, 712, 828, 1101, 1113, 1202, 1321, 2401
Ppu21I	YACGTR	3 cut(s)	280, 1489, 2708
PpuMI	RGGWCCY	1 cut(s)	2824
PscI	ACATGT	2 cut(s)	1968, 2010
PsiI	TTATAA	1 cut(s)	908
Psp124BI	GAGCTC	1 cut(s)	639
Psp5II	RGGWCCY	1 cut(s)	2824
Psp6I	CCWGG	7 cut(s)	82, 126, 330, 1582, 1945, 2074, 2633
PspCI	CACGTG	2 cut(s)	1489, 2708
PspFI	CCCAGC	1 cut(s)	606
PspGI	CCWGG	7 cut(s)	82, 126, 330, 1582, 1945, 2074, 2633
PspPI	GGNCC	8 cut(s)	80, 122, 501, 528, 609, 2246, 2415, 2824
PspPPI	RGGWCCY	1 cut(s)	2824
PstNI	CAGNNNCTG	3 cut(s)	472, 1102, 1114
PsuI	RGATCY	1 cut(s)	358
PsyI	GACNNNGTC	4 cut(s)	163, 311, 532, 1001
PvuI	CGATCG	1 cut(s)	526
RsaI	GTAC	9 cut(s)	47, 229, 1169, 1244, 1259, 1547, 2009, 2618, 2846
RsaNI	GTAC	9 cut(s)	46, 228, 1168, 1243, 1258, 1546, 2008, 2617, 2845
RseI	CAYNNNNRTG	4 cut(s)	692, 2363, 2612, 2651
SacI	GAGCTC	1 cut(s)	639
SapI	GCTCTTC	3 cut(s)	551, 2171, 2363
SaqAI	TTAA	3 cut(s)	198, 1338, 1436
Sau3AI	GATC	7 cut(s)	285, 358, 523, 975, 1350, 1449, 2586
Sau96I	GGNCC	8 cut(s)	80, 122, 501, 528, 609, 2246, 2415, 2824
ScaI	AGTACT	1 cut(s)	1169
SchI	GAGTC	9 cut(s)	71, 302, 713, 828, 1102, 1114, 1203, 1321, 2402
SduI	GDGCHC	6 cut(s)	639, 739, 2260, 2307, 2705, 2809
SfaNI	GCATC	7 cut(s)	18, 102, 1139, 1197, 1806, 1917, 2620
SfoI	GGCGCC	3 cut(s)	589, 595, 2741
SfuI	TTCGAA	3 cut(s)	102, 2043, 2104
SgrAI	CRCCGGYG	2 cut(s)	338, 590
SinI	GGWCC	3 cut(s)	528, 2246, 2824
SmiMI	CAYNNNNRTG	4 cut(s)	692, 2363, 2612, 2651
SmlI	CTYRAG	5 cut(s)	563, 1331, 1715, 1928, 2828
SmoI	CTYRAG	5 cut(s)	563, 1331, 1715, 1928, 2828
Sse9I	AATT	8 cut(s)	172, 794, 1024, 1377, 1403, 1438, 2834, 2890
SseBI	AGGCCT	1 cut(s)	2495
SspDI	GGCGCC	3 cut(s)	587, 593, 2739
SspMI	CTAG	3 cut(s)	989, 2220, 2402
SstI	GAGCTC	1 cut(s)	639
StuI	AGGCCT	1 cut(s)	2495
StyI	CCWWGG	5 cut(s)	708, 1676, 1792, 1815, 2325
TaaI	ACNGT	4 cut(s)	8, 2140, 2246, 2801
TaiI	ACGT	8 cut(s)	167, 282, 443, 1491, 1508, 1667, 2289, 2710
TasI	AATT	8 cut(s)	172, 794, 1024, 1377, 1403, 1438, 2834, 2890
TatI	WGTACW	5 cut(s)	45, 1167, 1242, 2007, 2844
TauI	GCSGC	9 cut(s)	123, 338, 481, 614, 1714, 2128, 2184, 2412, 2769
TfiI	GAWTC	8 cut(s)	827, 879, 1464, 1924, 2310, 2387, 2548, 2593
Tru1I	TTAA	3 cut(s)	198, 1338, 1436
Tru9I	TTAA	3 cut(s)	198, 1338, 1436
TscAI	CASTG	6 cut(s)	396, 889, 1968, 2115, 2458, 2617
TseFI	GTSAC	2 cut(s)	895, 2509
TseI	GCWGC	7 cut(s)	90, 968, 1042, 1579, 2027, 2128, 2448
Tsp45I	GTSAC	2 cut(s)	895, 2509
TspDTI	ATGAA	5 cut(s)	17, 559, 710, 831, 2381
TspGWI	ACGGA	4 cut(s)	245, 301, 1534, 2268
TspRI	CASTG	6 cut(s)	396, 889, 1968, 2115, 2458, 2617
Tth111I	GACNNNGTC	4 cut(s)	163, 311, 532, 1001
Van91I	CCANNNNNTGG	3 cut(s)	1821, 2168, 2171
VpaK11BI	GGWCC	3 cut(s)	528, 2246, 2824
XagI	CCTNNNNNAGG	1 cut(s)	1814
XapI	RAATTY	4 cut(s)	794, 1024, 1438, 2890
XceI	RCATGY	3 cut(s)	1532, 1972, 2014
XcmI	CCANNNNNNNNNTGG	2 cut(s)	338, 388
XmiI	GTMKAC	1 cut(s)	618
XmnI	GAANNNNTTC	1 cut(s)	518
XspI	CTAG	3 cut(s)	989, 2220, 2402
ZrmI	AGTACT	1 cut(s)	1169
Zsp2I	ATGCAT	1 cut(s)	689

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.