Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0011670

Physical Location & Seq

Genomic Coordinates Forward (+)

59678901 .. 59683427

Gene Structure 4527 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG281800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2802 bp

ATGGGAAGCCGTCACGAGGATGGAGCTTCCGCGGAGAACAACTCCGACTCCGAGGTCATGGCGAGAGTGGAGAAGAAGGCTTTCAGCGGTCCTCTAGGCTCGAGCTCCTCCTCCAGGGCTTGGAGGAGGATCAAGAGCTTGGATAGGAAGAGGACTAGTAGTACTAGCACCGAAAACGATGACGACGAGGCCTACGTGGAGATAACTCTCGACATCCTCGACGACTCGGTGGCCGTGCACAGTGTTCAGGCCGCCGGAGGCGGTGGCCACGAGGACCCGGAGCTTGCCTTGCTGGCCAAGAAGACTCTGGAAGGCAAGAAGTCGGCGTCGTTCCGGTCGTCTCTCCTCCGCAACACTTCGACGCACATACGGCAGGTCTCGCAAGAGCTGAAGCGTCTGGCTTCATTCTCGAAGCGAAACTCCGGCGCCGGCCGACGTTTCGACCGGACCAGGTCCGCCACTGCACAAGCCTTGAAGAGCCTCAAGTTCATCACCGCCAAGGCCTCCTCCACCAACGGCTGGGCCACCGTCGAGAAACGTTTCGACGACCTTACCGCCACCTCCAATGGCCTCCTCAACTCTTCTCTCTTCGGTGAATGCATAGGAATGAACAAGGAGTCTAAGGAGTTCGCCGGCGAGCTTTTCCGGGCACTTGCTCGGAGACGCAACATTAGTGGTGATTCCATCAACAAGGCACAGCTTAGAGAATTCTGGGAGCAGATTTCTGACGAGAGCTTCGACTCTAGGCTCCAGACTTTCTTTGACATGGTGGATAAAGACGCCGATGGAAGAATCACAGAAGAAGAAGTCAGAGAGATTATTAGTCTCAGTGCTTCTGCGAACAAACTCTCCAACATTCAGAAACAAGCTCAGGAATATGCAGCGCTCATTATGGAAGAGCTTGACCCAGACAATGTTGGCTACATTATGGTTGAAAACTTGGAAACTTTGTTGCTGCAAGCTCCGGTGCACAAATCGGTCACGGTAAGCGACAGCCGAGTTCTGAGTCAGCTACTGAGTCAGAAGCTGAAGCCAACACAAGAAAACAACCCAATTACAAGATGGTACCAGAAGACCAAGTACTTCTTGTTAGATAATTGGCAAAGAGTTTGGGTTATGATGCTATGGATTGGGATAGTCTCAGCTTTGTTTGTGTACAAGTTTATTCAGTACAAGAATAAGGAAGCATTTGAAGTGATGGGCTATTGTGTTTGCATAGCCAAAGGAGGAGCAGAGACTTTGAAATTCAACATGGCACTGATTTTGTTACCGGTTTGTCGAAACACAATTACTTGGCTCAGAAACAAGACCAAATTGGGGGTGGTAGTCCCGTTCGATGATAATCTGAACTTTCATAAGGTCATTGCAGTTGGAATAGTCATCGGTGTTGGATTACACGCCGGAGCACATTTGACGTGTGATTTTCCGAGGCTGATTCACGCAACGGAAGAACAGTACGAGCCGATGATACCATACTTTGGTAAAGACCAGCCTGAGAATTACTGGTGGTTTGTGAAGGGAGTGGAAGGGTTGACTGGGATTGGGATTGTGGTGTTGATGACTATAGCATTCACTTTGGCCACTCCTTGGTTTAGAAGAAACAAGTTGAACTTGCCAAAGCCGCTGAAGAAACTCACAGGTTTCAACGCCTTTTGGTATTCACACCACCTATTTGTGATTGTCTACACTCTCCTCATAATCCATAGCATCAAACTCTACCTCGCTAAGAAATGGTACTACAAAACGACATGGATGTATTTGGCAGTGCCGGTTTTCCTCTACGCTTGTGAAAGGCTGATCAGATTTTTTAGATCAAGCATCAAGCCAGTGAAGATTCTCAAGGTTGCTGTTTATCCGGGAAATGTGCTTGCACTGCACATGTCTAAGCCGCAAGGCTTCAAATACAAGAGCGGGCAGTACATGTTCGTCAACTGCGCCGCAGTTTCTCCATTCGAATGGCATCCATTTTCAATTACCTCTTCACCGGGAGATGACTACCTGAGCGTCCACATTCGAACACTCGGGGATTGGACTGGGCAGCTCAAGACCGTTTTCTCATTGGTATGTCAGCCTTCAACTGCTGGTAAAAGTGGGCTACTCAGAGCTGACATGCAAGAAGAAAACAACCCCAGCTTTCCGAAGATACTAATCGACGGTCCATACGGAGCACCAGCACAAGACTACAAGAAATACGACGTTGTGTTGCTGGTCGGCCTCGGAATCGGGGCCACGCCCATGGTCAGCATTGTCAAAGACATCATCAACAACATGAATTTCTTGAAGAACAAGGAACTAGACGAGTACGCAGTTTTGGACGATGTACTAGAGAGTGGCAAGGCTGTTAATAACAACAGCAGCAGTAGTGGCAAGAGCAATAAAGGGTTCAGGACCCAGAAAGCTTATTACTATTGGGTCACGAGAGAACAGGGCTCGTTTGAATGGTTCAAAGGGATTCTCAATGAGGTGGCAGAGATGGACGAAAAGGGTGTGATAGAGTTGCACAATTACTGTACCAGTGTGTACGAGGAAGGTGATGCCAGGTCAGCACTGATAGCCATGCTTCAGAATCTCCACCATGCCAAGAATGGCGTAGATGTAGTCTCTGGGACGCGCGTCAAATCCCATTTCGCCAAACCCAATTGGCGTCAAGTCTACAAGAAAATCGCGCTGCATCATCCTGATAGCCGAGTTGGTGTGTTTTACTGTGGGGCACCGGCACTAACAAAGGAGCTGAGGCAATTGGCGCTGGACTTTTCTCACAGGACCTCCACCAAGTTCGAGTTTCATAAAGAGAATTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005886 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0007231 GO:0008150 GO:0008152 GO:0009266 GO:0009408 GO:0009605 GO:0009607 GO:0009611 GO:0009620 GO:0009628 GO:0009987 GO:0010941 GO:0012505 GO:0016020 GO:0016174 GO:0016491 GO:0016651 GO:0023052 GO:0033500 GO:0033554 GO:0042592 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0048519 GO:0048523 GO:0048878 GO:0050664 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055114 GO:0060548 GO:0065007 GO:0065008 GO:0071214 GO:0071470 GO:0071944 GO:0072593 GO:0098542 GO:0104004

KEGG Pathways

Metabolic & Signaling

ko04016 ko04626 map04016 map04626

Pfam Domains

Protein Families

FAD_binding_8 Ferric_reduct NADPH_Ox NAD_binding_6

Protein Analysis

933

Amino Acids

105.35

Weight (kDa)

9.09

Isoelectric Point (pI)

39.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NADPH_Ox	PF08414	146 - 248	3.2e-37	Respiratory burst NADPH oxidase
EF-hand_7	PF13499	248 - 314	3.7e-06	EF-hand domain pair
Ferric_reduct	PF01794	418 - 565	6.7e-12	Ferric reductase like transmembrane component
FAD_binding_8	PF08022	609 - 725	4.7e-36	FAD-binding domain
NAD_binding_6	PF08030	731 - 915	4.5e-49	Ferric reductase NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011670)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G47910
fragaria_vesca	FvH4_4g22100 FvH4_4g22100
malus_domestica	MD13G1134500.v1.1 MD16G1145100.v1.1
prunus_persica	Prupe.1G211000_v2.0.a1
pyrus_communis	pycom13g11730 pycom16g11470
rosa_chinensis	RchiOBHm_Chr4g0428361
rosa_laevigata	RLG00000007160
rosa_multiflora	Rmu_co8508821.1_g000001 Rmu_sc0003384.1_g000003 Rmu_sc0010192.1_g000005
rosa_roxburghii	Rroxscaffold_5G00370020
rosa_rugosa	Rorug04G0225000
rosa_samantha	Rh4AG281800 Rh4CG303200 Rh4DG284600
rosa_wichuraiana	Rw4G024470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	53
Acc36I	ACCTGC	1 cut(s)	364
Acc65I	GGTACC	1 cut(s)	1065
AccB1I	GGYRCC	3 cut(s)	425, 1065, 2710
AccB7I	CCANNNNNTGG	4 cut(s)	120, 519, 1478, 1587
AccBSI	CCGCTC	1 cut(s)	1911
AccI	GTMKAC	2 cut(s)	1683, 2652
AccII	CGCG	4 cut(s)	32, 2611, 2613, 2666
AclI	AACGTT	1 cut(s)	538
AclWI	GGATC	1 cut(s)	137
AcoI	YGGCCR	5 cut(s)	231, 265, 294, 430, 1578
AcsI	RAATTY	4 cut(s)	707, 1244, 2272, 2794
AcuI	CTGAAG	4 cut(s)	410, 1049, 1646, 2546
AcyI	GRCGYC	4 cut(s)	326, 426, 780, 2644
AfeI	AGCGCT	1 cut(s)	885
AfiI	CCNNNNNNNGG	7 cut(s)	16, 114, 120, 519, 1317, 1478, 1587
AflIII	ACRYGT	3 cut(s)	1415, 1878, 1920
AgeI	ACCGGT	1 cut(s)	1270
AhlI	ACTAGT	1 cut(s)	155
AjiI	CACGTC	1 cut(s)	1416
AjnI	CCWGG	3 cut(s)	113, 449, 2537
AloI	GAACNNNNNNTCC	2 cut(s)	833, 865
Alw21I	GWGCWC	5 cut(s)	107, 240, 972, 1408, 2170
Alw26I	GTCTC	7 cut(s)	345, 382, 655, 830, 1144, 1229, 2605
Alw44I	GTGCAC	2 cut(s)	236, 968
AlwI	GGATC	1 cut(s)	137
AlwNI	CAGNNNCTG	2 cut(s)	1015, 1027
Ama87I	CYCGRG	2 cut(s)	100, 2021
Aor51HI	AGCGCT	1 cut(s)	885
ApaLI	GTGCAC	2 cut(s)	236, 968
ApeKI	GCWGC	5 cut(s)	881, 955, 2038, 2355, 2668
ApoI	RAATTY	4 cut(s)	707, 1244, 2272, 2794
ArsI	GACNNNNNNTTYG	2 cut(s)	719, 751
AsiGI	ACCGGT	1 cut(s)	1270
Asp700I	GAANNNNTTC	2 cut(s)	80, 539
Asp718I	GGTACC	1 cut(s)	1065
AspLEI	GCGC	6 cut(s)	428, 886, 1937, 2613, 2668, 2746
AspS9I	GGNCC	9 cut(s)	89, 274, 447, 453, 522, 2156, 2226, 2388, 2763
AsuC2I	CCSGG	4 cut(s)	278, 647, 1857, 1986
AsuHPI	GGTGA	5 cut(s)	484, 605, 689, 1974, 2543
AsuII	TTCGAA	2 cut(s)	1953, 2014
AvaI	CYCGRG	2 cut(s)	100, 2021
AvaII	GGWCC	7 cut(s)	89, 274, 447, 453, 2156, 2388, 2763
BaeGI	GKGCMC	4 cut(s)	240, 652, 972, 2713
BalI	TGGCCA	3 cut(s)	267, 296, 1580
BanI	GGYRCC	3 cut(s)	425, 1065, 2710
BanII	GRGCYC	2 cut(s)	107, 2432
BarI	GAAGNNNNNNTAC	2 cut(s)	1064, 1096
BauI	CACGAG	3 cut(s)	14, 269, 2416
BbsI	GAAGAC	2 cut(s)	308, 1079
Bbv12I	GWGCWC	5 cut(s)	107, 240, 972, 1408, 2170
BbvCI	CCTCAGC	1 cut(s)	2732
BbvI	GCAGC	5 cut(s)	893, 942, 2050, 2367, 2655
BccI	CCATC	6 cut(s)	14, 692, 779, 1056, 1192, 2467
BceAI	ACGGC	3 cut(s)	218, 386, 532
BciT130I	CCWGG	3 cut(s)	115, 451, 2539
BclI	TGATCA	1 cut(s)	1797
BcnI	CCSGG	4 cut(s)	278, 647, 1857, 1986
BcoDI	GTCTC	7 cut(s)	345, 382, 655, 830, 1144, 1229, 2605
BcuI	ACTAGT	1 cut(s)	155
BfaI	CTAG	7 cut(s)	95, 156, 165, 744, 2294, 2324, 2800
BfmI	CTRYAG	1 cut(s)	1563
BfoI	RGCGCY	3 cut(s)	429, 887, 2747
BfuAI	ACCTGC	1 cut(s)	364
BglI	GCCNNNNNGGC	1 cut(s)	293
BisI	GCNGC	9 cut(s)	252, 882, 956, 1622, 1889, 1938, 2039, 2356, 2669
BlsI	GCNGC	9 cut(s)	253, 883, 957, 1623, 1890, 1939, 2040, 2357, 2670
BmcAI	AGTACT	2 cut(s)	163, 1082
Bme1390I	CCNGG	7 cut(s)	115, 278, 451, 647, 1857, 1986, 2539
Bme18I	GGWCC	7 cut(s)	89, 274, 447, 453, 2156, 2388, 2763
BmeT110I	CYCGRG	2 cut(s)	100, 2021
BmgBI	CACGTC	1 cut(s)	1416
BmgT120I	GGNCC	9 cut(s)	89, 274, 447, 453, 522, 2156, 2226, 2388, 2763
BmiI	GGNNCC	7 cut(s)	276, 427, 749, 1067, 2227, 2390, 2712
BmrFI	CCNGG	7 cut(s)	115, 278, 451, 647, 1857, 1986, 2539
BmrI	ACTGGG	2 cut(s)	1545, 2043
BmsI	GCATC	6 cut(s)	1110, 1716, 1827, 1969, 2524, 2680
BmuI	ACTGGG	2 cut(s)	1545, 2043
BoxI	GACNNNNGTC	1 cut(s)	2612
BpiI	GAAGAC	2 cut(s)	308, 1079
BplI	GAGNNNNNCTC	2 cut(s)	26, 58
BpmI	CTGGAG	2 cut(s)	97, 734
Bpu10I	CCTNAGC	3 cut(s)	870, 2000, 2732
Bpu14I	TTCGAA	2 cut(s)	1953, 2014
BpuEI	CTTGAG	3 cut(s)	467, 1823, 2027
BpuMI	CCSGG	4 cut(s)	278, 647, 1857, 1986
BsaAI	YACGTR	1 cut(s)	196
BsaBI	GATNNNNATC	2 cut(s)	1341, 2147
BsaHI	GRCGYC	4 cut(s)	326, 426, 780, 2644
BsaI	GGTCTC	1 cut(s)	382
BsaJI	CCNNGG	8 cut(s)	30, 51, 114, 498, 1427, 1586, 2215, 2235
BsaWI	WCCGGW	4 cut(s)	333, 444, 964, 1270
BsaXI	ACNNNNNCTCC	6 cut(s)	32, 62, 833, 863, 2750, 2780
Bsc4I	CCNNNNNNNGG	7 cut(s)	16, 114, 120, 519, 1317, 1478, 1587
Bse118I	RCCGGY	5 cut(s)	428, 632, 1270, 1768, 2713
Bse1I	ACTGG	5 cut(s)	1508, 1540, 1826, 2038, 2514
Bse3DI	GCAATG	1 cut(s)	1362
Bse8I	GATNNNNATC	2 cut(s)	1341, 2147
BseBI	CCWGG	3 cut(s)	115, 451, 2539
BseDI	CCNNGG	8 cut(s)	30, 51, 114, 498, 1427, 1586, 2215, 2235
BseGI	GGATG	5 cut(s)	25, 213, 1758, 1960, 2674
BseJI	GATNNNNATC	2 cut(s)	1341, 2147
BseLI	CCNNNNNNNGG	7 cut(s)	16, 114, 120, 519, 1317, 1478, 1587
BseMI	GCAATG	1 cut(s)	1362
BseNI	ACTGG	5 cut(s)	1508, 1540, 1826, 2038, 2514
BseRI	GAGGAG	8 cut(s)	97, 100, 139, 335, 496, 563, 1242, 1682
BseSI	GKGCMC	4 cut(s)	240, 652, 972, 2713
BseX3I	CGGCCG	1 cut(s)	430
BseXI	GCAGC	5 cut(s)	893, 942, 2050, 2367, 2655
BseYI	CCCAGC	2 cut(s)	519, 2129
BsgI	GTGCAG	2 cut(s)	447, 1859
Bsh1236I	CGCG	4 cut(s)	32, 2611, 2613, 2666
Bsh1285I	CGRYCG	3 cut(s)	338, 433, 445
BshNI	GGYRCC	3 cut(s)	425, 1065, 2710
BshTI	ACCGGT	1 cut(s)	1270
BsiEI	CGRYCG	3 cut(s)	338, 433, 445
BsiHKAI	GWGCWC	5 cut(s)	107, 240, 972, 1408, 2170
BsiHKCI	CYCGRG	2 cut(s)	100, 2021
BslFI	GGGAC	2 cut(s)	1313, 2620
BslI	CCNNNNNNNGG	7 cut(s)	16, 114, 120, 519, 1317, 1478, 1587
BsmAI	GTCTC	7 cut(s)	345, 382, 655, 830, 1144, 1229, 2605
BsmBI	CGTCTC	2 cut(s)	345, 655
BsmFI	GGGAC	2 cut(s)	1313, 2620
BsmI	GAATGC	2 cut(s)	602, 1568
Bso31I	GGTCTC	1 cut(s)	382
BsoBI	CYCGRG	2 cut(s)	100, 2021
Bsp119I	TTCGAA	2 cut(s)	1953, 2014
Bsp1286I	GDGCHC	8 cut(s)	107, 240, 652, 972, 1408, 2170, 2432, 2713
Bsp1407I	TGTACA	1 cut(s)	1155
Bsp143I	GATC	3 cut(s)	129, 1797, 1811
Bsp19I	CCATGG	1 cut(s)	2235
BspFNI	CGCG	4 cut(s)	32, 2611, 2613, 2666
BspLI	GGNNCC	7 cut(s)	276, 427, 749, 1067, 2227, 2390, 2712
BspMI	ACCTGC	1 cut(s)	364
BspPI	GGATC	1 cut(s)	137
BspQI	GCTCTTC	2 cut(s)	470, 891
BspT104I	TTCGAA	2 cut(s)	1953, 2014
BspT107I	GGYRCC	3 cut(s)	425, 1065, 2710
BspTNI	GGTCTC	1 cut(s)	382
BsrBI	CCGCTC	1 cut(s)	1911
BsrDI	GCAATG	1 cut(s)	1362
BsrFI	RCCGGY	5 cut(s)	428, 632, 1270, 1768, 2713
BsrGI	TGTACA	1 cut(s)	1155
BsrI	ACTGG	5 cut(s)	1508, 1540, 1826, 2038, 2514
BssAI	RCCGGY	5 cut(s)	428, 632, 1270, 1768, 2713
BssECI	CCNNGG	8 cut(s)	30, 51, 114, 498, 1427, 1586, 2215, 2235
BssMI	GATC	3 cut(s)	129, 1797, 1811
BssNI	GRCGYC	4 cut(s)	326, 426, 780, 2644
BssSI	CACGAG	3 cut(s)	14, 269, 2416
BssT1I	CCWWGG	3 cut(s)	498, 1586, 2235
Bst2BI	CACGAG	3 cut(s)	14, 269, 2416
Bst2UI	CCWGG	3 cut(s)	115, 451, 2539
Bst4CI	ACNGT	8 cut(s)	242, 529, 985, 1455, 2050, 2156, 2510, 2705
Bst6I	CTCTTC	6 cut(s)	143, 470, 586, 593, 891, 1984
BstACI	GRCGYC	4 cut(s)	326, 426, 780, 2644
BstAUI	TGTACA	1 cut(s)	1155
BstBAI	YACGTR	1 cut(s)	196
BstBI	TTCGAA	2 cut(s)	1953, 2014
BstC8I	GCNNGC	8 cut(s)	285, 294, 430, 634, 638, 960, 1869, 1913
BstDSI	CCRYGG	2 cut(s)	30, 2235
BstENI	CCTNNNNNAGG	1 cut(s)	112
BstF5I	GGATG	5 cut(s)	25, 213, 1758, 1960, 2674
BstFNI	CGCG	4 cut(s)	32, 2611, 2613, 2666
BstH2I	RGCGCY	3 cut(s)	429, 887, 2747
BstHHI	GCGC	6 cut(s)	428, 886, 1937, 2613, 2668, 2746
BstKTI	GATC	3 cut(s)	132, 1800, 1814
BstMAI	GTCTC	7 cut(s)	345, 382, 655, 830, 1144, 1229, 2605
BstMBI	GATC	3 cut(s)	129, 1797, 1811
BstMCI	CGRYCG	3 cut(s)	338, 433, 445
BstNI	CCWGG	3 cut(s)	115, 451, 2539
BstNSI	RCATGY	3 cut(s)	1882, 1924, 2113
BstPAI	GACNNNNGTC	1 cut(s)	2612
BstSCI	CCNGG	7 cut(s)	113, 276, 449, 645, 1855, 1984, 2537
BstSFI	CTRYAG	1 cut(s)	1563
BstSLI	GKGCMC	4 cut(s)	240, 652, 972, 2713
BstUI	CGCG	4 cut(s)	32, 2611, 2613, 2666
BstV1I	GCAGC	5 cut(s)	893, 942, 2050, 2367, 2655
BstV2I	GAAGAC	2 cut(s)	308, 1079
BstXI	CCANNNNNNTGG	2 cut(s)	1956, 2236
BstZI	CGGCCG	1 cut(s)	430
BtgI	CCRYGG	2 cut(s)	30, 2235
BtrI	CACGTC	1 cut(s)	1416
BtsCI	GGATG	5 cut(s)	25, 213, 1758, 1960, 2674
BtsI	GCAGTG	3 cut(s)	459, 1770, 1871
BtsIMutI	CAGTG	9 cut(s)	247, 459, 835, 1256, 1770, 1833, 1871, 2521, 2546
BveI	ACCTGC	1 cut(s)	364
Cac8I	GCNNGC	8 cut(s)	285, 294, 430, 634, 638, 960, 1869, 1913
CaiI	CAGNNNCTG	2 cut(s)	1015, 1027
CfoI	GCGC	6 cut(s)	428, 886, 1937, 2613, 2668, 2746
Cfr10I	RCCGGY	5 cut(s)	428, 632, 1270, 1768, 2713
Cfr13I	GGNCC	9 cut(s)	89, 274, 447, 453, 522, 2156, 2226, 2388, 2763
Cfr42I	CCGCGG	1 cut(s)	33
CseI	GACGC	9 cut(s)	315, 370, 383, 672, 788, 1993, 2602, 2617, 2633
CsiI	ACCWGGT	1 cut(s)	449
CspAI	ACCGGT	1 cut(s)	1270
CspCI	CAANNNNNGTGG	2 cut(s)	655, 690
DinI	GGCGCC	1 cut(s)	427
DpnI	GATC	3 cut(s)	131, 1799, 1813
DpnII	GATC	3 cut(s)	129, 1797, 1811
DrdI	GACNNNNNNGTC	1 cut(s)	53
DseDI	GACNNNNNNGTC	1 cut(s)	53
EaeI	YGGCCR	5 cut(s)	231, 265, 294, 430, 1578
EagI	CGGCCG	1 cut(s)	430
Eam1104I	CTCTTC	6 cut(s)	143, 470, 586, 593, 891, 1984
EarI	CTCTTC	6 cut(s)	143, 470, 586, 593, 891, 1984
EciI	GGCGGA	1 cut(s)	445
Ecl136II	GAGCTC	1 cut(s)	105
EclXI	CGGCCG	1 cut(s)	430
Eco130I	CCWWGG	3 cut(s)	498, 1586, 2235
Eco147I	AGGCCT	2 cut(s)	191, 503
Eco24I	GRGCYC	2 cut(s)	107, 2432
Eco31I	GGTCTC	1 cut(s)	382
Eco47I	GGWCC	7 cut(s)	89, 274, 447, 453, 2156, 2388, 2763
Eco47III	AGCGCT	1 cut(s)	885
Eco52I	CGGCCG	1 cut(s)	430
Eco53kI	GAGCTC	1 cut(s)	105
Eco57I	CTGAAG	4 cut(s)	410, 1049, 1646, 2546
Eco88I	CYCGRG	2 cut(s)	100, 2021
EcoICRI	GAGCTC	1 cut(s)	105
EcoNI	CCTNNNNNAGG	1 cut(s)	112
EcoO109I	RGGNCCY	3 cut(s)	274, 2388, 2763
EcoRI	GAATTC	1 cut(s)	707
EcoRII	CCWGG	3 cut(s)	113, 449, 2537
EcoT14I	CCWWGG	3 cut(s)	498, 1586, 2235
EcoT22I	ATGCAT	1 cut(s)	602
EcoT38I	GRGCYC	2 cut(s)	107, 2432
EgeI	GGCGCC	1 cut(s)	427
EheI	GGCGCC	1 cut(s)	427
ErhI	CCWWGG	3 cut(s)	498, 1586, 2235
Esp3I	CGTCTC	2 cut(s)	345, 655
FalI	AAGNNNNNCTT	4 cut(s)	1070, 1102, 1595, 1627
FaqI	GGGAC	2 cut(s)	1313, 2620
FauI	CCCGC	1 cut(s)	1904
FbaI	TGATCA	1 cut(s)	1797
FblI	GTMKAC	2 cut(s)	1683, 2652
Fnu4HI	GCNGC	9 cut(s)	252, 882, 956, 1622, 1889, 1938, 2039, 2356, 2669
FokI	GGATG	5 cut(s)	32, 200, 1765, 1947, 2661
FriOI	GRGCYC	2 cut(s)	107, 2432
Fsp4HI	GCNGC	9 cut(s)	252, 882, 956, 1622, 1889, 1938, 2039, 2356, 2669
FspBI	CTAG	7 cut(s)	95, 156, 165, 744, 2294, 2324, 2800
GlaI	GCGC	6 cut(s)	427, 885, 1936, 2612, 2667, 2745
GluI	GCNGC	9 cut(s)	252, 882, 956, 1622, 1889, 1938, 2039, 2356, 2669
GsaI	CCCAGC	2 cut(s)	523, 2133
GsuI	CTGGAG	2 cut(s)	97, 734
HaeII	RGCGCY	3 cut(s)	429, 887, 2747
HgaI	GACGC	9 cut(s)	315, 370, 383, 672, 788, 1993, 2602, 2617, 2633
HhaI	GCGC	6 cut(s)	428, 886, 1937, 2613, 2668, 2746
Hin1I	GRCGYC	4 cut(s)	326, 426, 780, 2644
Hin6I	GCGC	6 cut(s)	426, 884, 1935, 2611, 2666, 2744
HinP1I	GCGC	6 cut(s)	426, 884, 1935, 2611, 2666, 2744
HincII	GTYRAC	2 cut(s)	1533, 1930
HindII	GTYRAC	2 cut(s)	1533, 1930
HindIII	AAGCTT	1 cut(s)	2397
HphI	GGTGA	5 cut(s)	484, 605, 689, 1974, 2543
Hpy166II	GTNNAC	9 cut(s)	238, 970, 1156, 1533, 1684, 1930, 2008, 2521, 2653
Hpy8I	GTNNAC	9 cut(s)	238, 970, 1156, 1533, 1684, 1930, 2008, 2521, 2653
Hpy99I	CGWCG	9 cut(s)	188, 224, 331, 364, 438, 533, 548, 2156, 2198
HpyAV	CCTTC	6 cut(s)	70, 305, 1510, 1520, 2082, 2522
HpyCH4III	ACNGT	8 cut(s)	242, 529, 985, 1455, 2050, 2156, 2510, 2705
HpyCH4IV	ACGT	5 cut(s)	195, 436, 538, 1415, 2196
HpySE526I	ACGT	5 cut(s)	195, 436, 538, 1415, 2196
Hsp92I	GRCGYC	4 cut(s)	326, 426, 780, 2644
HspAI	GCGC	6 cut(s)	426, 884, 1935, 2611, 2666, 2744
KasI	GGCGCC	1 cut(s)	425
KpnI	GGTACC	1 cut(s)	1069
KroI	GCCGGC	2 cut(s)	428, 632
KroNI	GCCGGC	2 cut(s)	430, 634
Ksp22I	TGATCA	1 cut(s)	1797
KspI	CCGCGG	1 cut(s)	33
Kzo9I	GATC	3 cut(s)	129, 1797, 1811
LguI	GCTCTTC	2 cut(s)	470, 891
Lsp1109I	GCAGC	5 cut(s)	893, 942, 2050, 2367, 2655
LweI	GCATC	6 cut(s)	1110, 1716, 1827, 1969, 2524, 2680
MabI	ACCWGGT	1 cut(s)	449
MaeI	CTAG	7 cut(s)	95, 156, 165, 744, 2294, 2324, 2800
MaeII	ACGT	5 cut(s)	195, 436, 538, 1415, 2196
MaeIII	GTNAC	4 cut(s)	11, 979, 1266, 2413
MalI	GATC	3 cut(s)	131, 1799, 1813
MbiI	CCGCTC	1 cut(s)	1911
MboI	GATC	3 cut(s)	129, 1797, 1811
MfeI	CAATTG	2 cut(s)	2638, 2738
MhlI	GDGCHC	8 cut(s)	107, 240, 652, 972, 1408, 2170, 2432, 2713
MlsI	TGGCCA	3 cut(s)	267, 296, 1580
MluNI	TGGCCA	3 cut(s)	267, 296, 1580
Mly113I	GGCGCC	1 cut(s)	426
MlyI	GAGTC	7 cut(s)	41, 218, 298, 626, 734, 1015, 1027
MmeI	TCCRAC	4 cut(s)	69, 876, 1351, 1369
Mox20I	TGGCCA	3 cut(s)	267, 296, 1580
Mph1103I	ATGCAT	1 cut(s)	602
MreI	CGCCGGCG	1 cut(s)	632
MroNI	GCCGGC	2 cut(s)	428, 632
MroXI	GAANNNNTTC	2 cut(s)	80, 539
MscI	TGGCCA	3 cut(s)	267, 296, 1580
MseI	TTAA	1 cut(s)	2343
MslI	CAYNNNNRTG	5 cut(s)	18, 605, 1954, 2062, 2234
Msp20I	TGGCCA	3 cut(s)	267, 296, 1580
MspA1I	CMGCKG	3 cut(s)	32, 87, 1624
MspR9I	CCNGG	7 cut(s)	115, 278, 451, 647, 1857, 1986, 2539
MunI	CAATTG	2 cut(s)	2638, 2738
Mva1269I	GAATGC	2 cut(s)	602, 1568
MvaI	CCWGG	3 cut(s)	115, 451, 2539
MvnI	CGCG	4 cut(s)	32, 2611, 2613, 2666
NaeI	GCCGGC	2 cut(s)	430, 634
NarI	GGCGCC	1 cut(s)	426
NciI	CCSGG	4 cut(s)	278, 647, 1857, 1986
NcoI	CCATGG	1 cut(s)	2235
NdeII	GATC	3 cut(s)	129, 1797, 1811
NgoMIV	GCCGGC	2 cut(s)	428, 632
NlaIV	GGNNCC	7 cut(s)	276, 427, 749, 1067, 2227, 2390, 2712
NmeAIII	GCCGAG	2 cut(s)	1022, 2711
NmuCI	GTSAC	3 cut(s)	11, 979, 2413
NsiI	ATGCAT	1 cut(s)	602
NspI	RCATGY	3 cut(s)	1882, 1924, 2113
NspV	TTCGAA	2 cut(s)	1953, 2014
PaeR7I	CTCGAG	1 cut(s)	100
PceI	AGGCCT	2 cut(s)	191, 503
PciI	ACATGT	2 cut(s)	1878, 1920
PciSI	GCTCTTC	2 cut(s)	470, 891
PcsI	WCGNNNNNNNCGW	5 cut(s)	183, 192, 216, 335, 735
PctI	GAATGC	2 cut(s)	602, 1568
PdiI	GCCGGC	2 cut(s)	430, 634
PdmI	GAANNNNTTC	2 cut(s)	80, 539
PfeI	GAWTC	7 cut(s)	680, 792, 1435, 1834, 2220, 2452, 2566
PflFI	GACNNNGTC	1 cut(s)	451
PflMI	CCANNNNNTGG	4 cut(s)	120, 519, 1478, 1587
PfoI	TCCNGGA	1 cut(s)	1855
PinAI	ACCGGT	1 cut(s)	1270
PkrI	GCNGC	9 cut(s)	253, 883, 957, 1623, 1890, 1939, 2040, 2357, 2670
PleI	GAGTC	7 cut(s)	41, 218, 298, 625, 734, 1014, 1026
PluTI	GGCGCC	1 cut(s)	429
PpsI	GAGTC	7 cut(s)	41, 218, 298, 625, 734, 1014, 1026
Ppu21I	YACGTR	1 cut(s)	196
PpuMI	RGGWCCY	3 cut(s)	274, 2388, 2763
PscI	ACATGT	2 cut(s)	1878, 1920
PshAI	GACNNNNGTC	1 cut(s)	2612
Psp124BI	GAGCTC	1 cut(s)	107
Psp1406I	AACGTT	1 cut(s)	538
Psp5II	RGGWCCY	3 cut(s)	274, 2388, 2763
Psp6I	CCWGG	3 cut(s)	113, 449, 2537
PspFI	CCCAGC	2 cut(s)	519, 2129
PspGI	CCWGG	3 cut(s)	113, 449, 2537
PspN4I	GGNNCC	7 cut(s)	276, 427, 749, 1067, 2227, 2390, 2712
PspPI	GGNCC	9 cut(s)	89, 274, 447, 453, 522, 2156, 2226, 2388, 2763
PspPPI	RGGWCCY	3 cut(s)	274, 2388, 2763
PspXI	VCTCGAGB	1 cut(s)	100
PstNI	CAGNNNCTG	2 cut(s)	1015, 1027
PsyI	GACNNNGTC	1 cut(s)	451
RseI	CAYNNNNRTG	5 cut(s)	18, 605, 1954, 2062, 2234
SacI	GAGCTC	1 cut(s)	107
SacII	CCGCGG	1 cut(s)	33
SapI	GCTCTTC	2 cut(s)	470, 891
SaqAI	TTAA	1 cut(s)	2343
SatI	GCNGC	9 cut(s)	252, 882, 956, 1622, 1889, 1938, 2039, 2356, 2669
Sau3AI	GATC	3 cut(s)	129, 1797, 1811
Sau96I	GGNCC	9 cut(s)	89, 274, 447, 453, 522, 2156, 2226, 2388, 2763
ScaI	AGTACT	2 cut(s)	163, 1082
SchI	GAGTC	7 cut(s)	41, 218, 298, 626, 734, 1015, 1027
ScrFI	CCNGG	7 cut(s)	115, 278, 451, 647, 1857, 1986, 2539
SduI	GDGCHC	8 cut(s)	107, 240, 652, 972, 1408, 2170, 2432, 2713
SexAI	ACCWGGT	1 cut(s)	449
SfaNI	GCATC	6 cut(s)	1110, 1716, 1827, 1969, 2524, 2680
SfcI	CTRYAG	1 cut(s)	1563
SfoI	GGCGCC	1 cut(s)	427
Sfr274I	CTCGAG	1 cut(s)	100
Sfr303I	CCGCGG	1 cut(s)	33
SfuI	TTCGAA	2 cut(s)	1953, 2014
SgrAI	CRCCGGYG	1 cut(s)	632
SgrBI	CCGCGG	1 cut(s)	33
SinI	GGWCC	7 cut(s)	89, 274, 447, 453, 2156, 2388, 2763
SlaI	CTCGAG	1 cut(s)	100
SmiMI	CAYNNNNRTG	5 cut(s)	18, 605, 1954, 2062, 2234
SmlI	CTYRAG	4 cut(s)	100, 482, 1838, 2042
SmoI	CTYRAG	4 cut(s)	100, 482, 1838, 2042
SpeI	ACTAGT	1 cut(s)	155
SseBI	AGGCCT	2 cut(s)	191, 503
SspDI	GGCGCC	1 cut(s)	425
SspMI	CTAG	7 cut(s)	95, 156, 165, 744, 2294, 2324, 2800
SstI	GAGCTC	1 cut(s)	107
StuI	AGGCCT	2 cut(s)	191, 503
StyD4I	CCNGG	7 cut(s)	113, 276, 449, 645, 1855, 1984, 2537
StyI	CCWWGG	3 cut(s)	498, 1586, 2235
TaaI	ACNGT	8 cut(s)	242, 529, 985, 1455, 2050, 2156, 2510, 2705
TaiI	ACGT	5 cut(s)	198, 439, 541, 1418, 2199
TaqII	GACCGA	1 cut(s)	967
TatI	WGTACW	6 cut(s)	161, 1080, 1155, 1170, 1917, 2320
TauI	GCSGC	4 cut(s)	254, 1624, 1891, 1940
TfiI	GAWTC	7 cut(s)	680, 792, 1435, 1834, 2220, 2452, 2566
Tru1I	TTAA	1 cut(s)	2343
Tru9I	TTAA	1 cut(s)	2343
TscAI	CASTG	9 cut(s)	247, 466, 835, 1263, 1770, 1833, 1878, 2521, 2553
TseFI	GTSAC	3 cut(s)	11, 979, 2413
TseI	GCWGC	5 cut(s)	881, 955, 2038, 2355, 2668
Tsp45I	GTSAC	3 cut(s)	11, 979, 2413
TspDTI	ATGAA	6 cut(s)	393, 478, 623, 1343, 2285, 2774
TspGWI	ACGGA	2 cut(s)	1460, 2178
TspRI	CASTG	9 cut(s)	247, 466, 835, 1263, 1770, 1833, 1878, 2521, 2553
Tth111I	GACNNNGTC	1 cut(s)	451
Van91I	CCANNNNNTGG	4 cut(s)	120, 519, 1478, 1587
VneI	GTGCAC	2 cut(s)	236, 968
VpaK11BI	GGWCC	7 cut(s)	89, 274, 447, 453, 2156, 2388, 2763
XagI	CCTNNNNNAGG	1 cut(s)	112
XapI	RAATTY	4 cut(s)	707, 1244, 2272, 2794
XceI	RCATGY	3 cut(s)	1882, 1924, 2113
XcmI	CCANNNNNNNNNTGG	4 cut(s)	304, 1059, 1318, 2582
XhoI	CTCGAG	1 cut(s)	100
XmiI	GTMKAC	2 cut(s)	1683, 2652
XmnI	GAANNNNTTC	2 cut(s)	80, 539
XspI	CTAG	7 cut(s)	95, 156, 165, 744, 2294, 2324, 2800
ZrmI	AGTACT	2 cut(s)	163, 1082
Zsp2I	ATGCAT	1 cut(s)	602

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.