FvH4_4g24850

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0015604
Physical Location & Seq
Forward (+)
26830688 .. 26831946
1259 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g24850.t1

Sequence Viewer

Length: 699 bp
ATGTCACAAGCTCTAGCCAACTTCAGCGTCTTTCTCCCTCTGTCATCTGGGAGAAGAAGAACCAAAAGTGTTGGTGGAGGTCTCACCTTGAAGGCCATGGCAAAGGAAGCAAGAGACAATCTCGACCACTTGCAGAGAGCAACCCAACAGCAGAGAGCAACCCAACATCAAAGCCAACCCAAGAAGAGAGTCGCTCCTACTCCGCCCGTAGGACTATGGGACCGGTTTCCAACTGCAAGAACAGTTCAGCAGATGATGGAGACGATGGAGAGAATGATGGACGACCCGTTGGTGTATTCGGGTCAGTCCGGCGGGTGGGCTCGGCCGTTGCCGACGGAGAGTGGCGGGTACAGCAGGGGAAGGACACCTTGGGAGATCAAAGAAGCTGAAACAGAGTACAAGATGAGGTTCGACATGCCTGGAATGACCAAAGAGGATGTGAAGGTTTGGGTGGAGGAGAAAATGCTAGTCGTTAAGGCTGAGAAGGCTGCCAAGAAGAAAGATGGTTCTGGAATGGAGGAAGAAGAAGAAGGTGAAGAGTGGTCGGCGAAGAGTTATGGCAGGTATAGTAGCCGGATTGCTCTGCCGGAGAATATTCAGTTTGAGAAGATTAAGGCTGAGGTGAAAGATGGGGTTTTGTATATCACTATTCCTAAAGCTATGAGCAGCTCCAAGATTTTGGACATCAATGTACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

233

Amino Acids

26.22

Weight (kDa)

9.24

Isoelectric Point (pI)

57.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 125 - 230 3.9e-23 Hsp20/alpha crystallin family
ArsA_HSP20 PF17886 131 - 216 4.3e-08 HSP20-like domain found in ArsA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015604)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24850
malus_domestica MD13G1108500.v1.1 MD16G1108600.v1.1
prunus_persica Prupe.1G237800_v2.0.a1
pyrus_communis pycom13g09400 pycom16g09270
rosa_chinensis RchiOBHm_Chr4g0431921
rosa_laevigata RLG00000006875
rosa_multiflora Rmu_sc0004965.1_g000008
rosa_roxburghii Rroxscaffold_5G00373350
rosa_rugosa Rorug04G0253000
rosa_samantha Rh4AG310300 Rh4BG317400 Rh4CG332700 Rh4DG312300
rosa_wichuraiana Rw4G027000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 552
AciI CCGC 3 cut(s) 203, 312, 345
AcoI YGGCCR 1 cut(s) 323
AcuI CTGAAG 1 cut(s) 7
AfaI GTAC 3 cut(s) 350, 398, 693
AfiI CCNNNNNNNGG 2 cut(s) 209, 315
AgeI ACCGGT 1 cut(s) 222
AgsI TTSAA 1 cut(s) 91
AjnI CCWGG 1 cut(s) 418
AjuI GAANNNNNNNTTGG 2 cut(s) 352, 384
AluBI AGCT 4 cut(s) 11, 386, 659, 669
AluI AGCT 4 cut(s) 11, 386, 659, 669
Alw26I GTCTC 3 cut(s) 86, 108, 254
AoxI GGCC 2 cut(s) 93, 323
ApeKI GCWGC 2 cut(s) 488, 666
AsiGI ACCGGT 1 cut(s) 222
AspS9I GGNCC 1 cut(s) 220
AsuHPI GGTGA 3 cut(s) 76, 545, 634
AvaII GGWCC 1 cut(s) 220
BanII GRGCYC 1 cut(s) 322
BbvCI CCTCAGC 1 cut(s) 618
BbvI GCAGC 2 cut(s) 475, 678
BccI CCATC 5 cut(s) 250, 259, 271, 497, 623
BceAI ACGGC 1 cut(s) 310
BciT130I CCWGG 1 cut(s) 420
BcoDI GTCTC 3 cut(s) 86, 108, 254
BfaI CTAG 2 cut(s) 14, 467
BfuAI ACCTGC 1 cut(s) 552
BisI GCNGC 2 cut(s) 489, 667
BlsI GCNGC 2 cut(s) 490, 668
Bme1390I CCNGG 1 cut(s) 420
Bme18I GGWCC 1 cut(s) 220
BmgT120I GGNCC 1 cut(s) 220
BmiI GGNNCC 1 cut(s) 221
BmrFI CCNGG 1 cut(s) 420
BplI GAGNNNNNCTC 4 cut(s) 105, 137, 178, 210
Bpu10I CCTNAGC 1 cut(s) 618
BsaI GGTCTC 1 cut(s) 86
BsaJI CCNNGG 2 cut(s) 96, 368
BsaWI WCCGGW 1 cut(s) 222
Bsc4I CCNNNNNNNGG 2 cut(s) 209, 315
Bse118I RCCGGY 1 cut(s) 222
BseBI CCWGG 1 cut(s) 420
BseDI CCNNGG 2 cut(s) 96, 368
BseGI GGATG 1 cut(s) 442
BseLI CCNNNNNNNGG 2 cut(s) 209, 315
BseMII CTCAG 2 cut(s) 471, 609
BseRI GAGGAG 1 cut(s) 470
BseX3I CGGCCG 1 cut(s) 323
BseXI GCAGC 2 cut(s) 475, 678
Bsh1285I CGRYCG 1 cut(s) 326
BshFI GGCC 2 cut(s) 95, 325
BshTI ACCGGT 1 cut(s) 222
BsiEI CGRYCG 1 cut(s) 326
BsiSI CCGG 4 cut(s) 223, 309, 574, 587
BslFI GGGAC 1 cut(s) 233
BslI CCNNNNNNNGG 2 cut(s) 209, 315
BsmAI GTCTC 3 cut(s) 86, 108, 254
BsmBI CGTCTC 1 cut(s) 254
BsmFI GGGAC 1 cut(s) 233
BsnI GGCC 2 cut(s) 95, 325
Bso31I GGTCTC 1 cut(s) 86
Bsp1286I GDGCHC 1 cut(s) 322
Bsp1407I TGTACA 1 cut(s) 691
Bsp143I GATC 1 cut(s) 375
Bsp19I CCATGG 1 cut(s) 96
BspACI CCGC 3 cut(s) 203, 312, 345
BspANI GGCC 2 cut(s) 95, 325
BspCNI CTCAG 2 cut(s) 472, 610
BspLI GGNNCC 1 cut(s) 221
BspMI ACCTGC 1 cut(s) 552
BspTNI GGTCTC 1 cut(s) 86
BsrFI RCCGGY 1 cut(s) 222
BsrGI TGTACA 1 cut(s) 691
BssAI RCCGGY 1 cut(s) 222
BssECI CCNNGG 2 cut(s) 96, 368
BssMI GATC 1 cut(s) 375
BssT1I CCWWGG 2 cut(s) 96, 368
Bst2UI CCWGG 1 cut(s) 420
Bst4CI ACNGT 1 cut(s) 244
Bst6I CTCTTC 3 cut(s) 179, 531, 545
BstAUI TGTACA 1 cut(s) 691
BstDEI CTNAG 2 cut(s) 480, 618
BstDSI CCRYGG 1 cut(s) 96
BstF5I GGATG 1 cut(s) 442
BstKTI GATC 1 cut(s) 378
BstMAI GTCTC 3 cut(s) 86, 108, 254
BstMBI GATC 1 cut(s) 375
BstMCI CGRYCG 1 cut(s) 326
BstMWI GCNNNNNNNGC 3 cut(s) 107, 351, 485
BstNI CCWGG 1 cut(s) 420
BstNSI RCATGY 1 cut(s) 418
BstSCI CCNGG 1 cut(s) 418
BstV1I GCAGC 2 cut(s) 475, 678
BstXI CCANNNNNNTGG 1 cut(s) 679
BstZI CGGCCG 1 cut(s) 323
BsuRI GGCC 2 cut(s) 95, 325
BtgI CCRYGG 1 cut(s) 96
BtsCI GGATG 1 cut(s) 442
BveI ACCTGC 1 cut(s) 552
Cfr10I RCCGGY 1 cut(s) 222
Cfr13I GGNCC 1 cut(s) 220
CseI GACGC 1 cut(s) 16
Csp6I GTAC 3 cut(s) 349, 397, 692
CspAI ACCGGT 1 cut(s) 222
CviAII CATG 2 cut(s) 97, 415
CviQI GTAC 3 cut(s) 349, 397, 692
DdeI CTNAG 2 cut(s) 480, 618
DpnI GATC 1 cut(s) 377
DpnII GATC 1 cut(s) 375
EaeI YGGCCR 1 cut(s) 323
EagI CGGCCG 1 cut(s) 323
Eam1104I CTCTTC 3 cut(s) 179, 531, 545
EarI CTCTTC 3 cut(s) 179, 531, 545
EciI GGCGGA 1 cut(s) 192
EclXI CGGCCG 1 cut(s) 323
Eco130I CCWWGG 2 cut(s) 96, 368
Eco24I GRGCYC 1 cut(s) 322
Eco31I GGTCTC 1 cut(s) 86
Eco47I GGWCC 1 cut(s) 220
Eco52I CGGCCG 1 cut(s) 323
Eco57I CTGAAG 1 cut(s) 7
EcoRII CCWGG 1 cut(s) 418
EcoT14I CCWWGG 2 cut(s) 96, 368
EcoT38I GRGCYC 1 cut(s) 322
ErhI CCWWGG 2 cut(s) 96, 368
Esp3I CGTCTC 1 cut(s) 254
FaeI CATG 2 cut(s) 100, 418
FaiI YATR 7 cut(s) 98, 217, 416, 558, 567, 642, 662
FalI AAGNNNNNCTT 2 cut(s) 352, 384
FaqI GGGAC 1 cut(s) 233
FatI CATG 2 cut(s) 96, 414
FauI CCCGC 2 cut(s) 305, 338
Fnu4HI GCNGC 2 cut(s) 489, 667
FokI GGATG 1 cut(s) 449
FriOI GRGCYC 1 cut(s) 322
Fsp4HI GCNGC 2 cut(s) 489, 667
FspBI CTAG 2 cut(s) 14, 467
GluI GCNGC 2 cut(s) 489, 667
HaeIII GGCC 2 cut(s) 95, 325
HapII CCGG 4 cut(s) 223, 309, 574, 587
HgaI GACGC 1 cut(s) 16
Hin1II CATG 2 cut(s) 100, 418
HinfI GANTC 1 cut(s) 189
HpaII CCGG 4 cut(s) 223, 309, 574, 587
HphI GGTGA 3 cut(s) 76, 545, 634
Hpy188III TCNNGA 2 cut(s) 122, 510
Hpy99I CGWCG 1 cut(s) 337
HpyAV CCTTC 5 cut(s) 85, 354, 436, 478, 524
HpyCH4III ACNGT 1 cut(s) 244
HpyCH4V TGCA 2 cut(s) 133, 236
HpyF10VI GCNNNNNNNGC 3 cut(s) 107, 351, 485
HpyF3I CTNAG 2 cut(s) 480, 618
Hsp92II CATG 2 cut(s) 100, 418
Kzo9I GATC 1 cut(s) 375
LmnI GCTCC 2 cut(s) 199, 674
Lsp1109I GCAGC 2 cut(s) 475, 678
MaeI CTAG 2 cut(s) 14, 467
MaeIII GTNAC 1 cut(s) 3
MalI GATC 1 cut(s) 377
MboI GATC 1 cut(s) 375
MhlI GDGCHC 1 cut(s) 322
MlyI GAGTC 1 cut(s) 198
MmeI TCCRAC 1 cut(s) 254
MnlI CCTC 7 cut(s) 48, 71, 399, 427, 448, 511, 613
MseI TTAA 2 cut(s) 474, 612
MspI CCGG 4 cut(s) 223, 309, 574, 587
MspR9I CCNGG 1 cut(s) 420
MvaI CCWGG 1 cut(s) 420
MwoI GCNNNNNNNGC 3 cut(s) 107, 351, 485
NcoI CCATGG 1 cut(s) 96
NdeII GATC 1 cut(s) 375
NlaIII CATG 2 cut(s) 100, 418
NlaIV GGNNCC 1 cut(s) 221
NmeAIII GCCGAG 1 cut(s) 301
NmuCI GTSAC 1 cut(s) 3
NspI RCATGY 1 cut(s) 418
PinAI ACCGGT 1 cut(s) 222
PkrI GCNGC 2 cut(s) 490, 668
PleI GAGTC 1 cut(s) 197
PpsI GAGTC 1 cut(s) 197
Psp6I CCWGG 1 cut(s) 418
PspGI CCWGG 1 cut(s) 418
PspN4I GGNNCC 1 cut(s) 221
PspPI GGNCC 1 cut(s) 220
RsaI GTAC 3 cut(s) 350, 398, 693
RsaNI GTAC 3 cut(s) 349, 397, 692
SaqAI TTAA 2 cut(s) 474, 612
SatI GCNGC 2 cut(s) 489, 667
Sau3AI GATC 1 cut(s) 375
Sau96I GGNCC 1 cut(s) 220
SchI GAGTC 1 cut(s) 198
ScrFI CCNGG 1 cut(s) 420
SduI GDGCHC 1 cut(s) 322
SinI GGWCC 1 cut(s) 220
SsiI CCGC 3 cut(s) 203, 312, 345
SspI AATATT 1 cut(s) 595
SspMI CTAG 2 cut(s) 14, 467
StyD4I CCNGG 1 cut(s) 418
StyI CCWWGG 2 cut(s) 96, 368
TaaI ACNGT 1 cut(s) 244
TaqI TCGA 2 cut(s) 123, 411
TatI WGTACW 2 cut(s) 396, 691
Tru1I TTAA 2 cut(s) 474, 612
Tru9I TTAA 2 cut(s) 474, 612
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 2 cut(s) 488, 666
Tsp45I GTSAC 1 cut(s) 3
TspGWI ACGGA 1 cut(s) 350
VpaK11BI GGWCC 1 cut(s) 220
XceI RCATGY 1 cut(s) 418
XspI CTAG 2 cut(s) 14, 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.