RLG00000006875

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0015604
Physical Location & Seq
Reverse (-)
11376529 .. 11377327
799 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006875

Sequence Viewer

Length: 705 bp
ATGTCACAAGCTCTATCCAATTTCAGCCTCTTTCTCCCTTTGTCGTTTGGGAGAAGAACCAAAACTCTTCCAATCAATGGTGGAGGTTTGAGCTTGAAGGCCATGGCAAACGAAGCCAGAGACAACCTCGACCACTTGCAGAGAGTCACCAAGCAACACCAACAACCACAGCAAAGCCGCCAACCCAAGAAGAGAGTCGCCCCTACCCCGCCTGTAGGACTATGGGACCGGTTTCCAACTGCAAGGACAGTTCAGCAGATGATGGAGACCATGGAGAGGATGATGGACGACCCGCTTGTCTATTCGGGTCAGTCGGGCGTGTGGGCTCCGCCGTTGCCGACCGAGACTGGCGGGTACAGCAGGGGAAGGACACCGTGGGAGATCAAAGAAGCTGAAACAGAGTACAAGATGAGGTTCGACATGCCCGGAATGACCAAAGAGGATGTCAAGATTTGGGTGGAGGAGAAAATGCTGGTCGTTAAGGCTGAGAAGGCGGTCAAGACGAAAGATGGTGGTGGAGTCGAGGAAGAAGAAGAAGGTGAAGAGTGGTCGGCTAAGAGTTATGGAAAGTATAGTAGTAGAATTGCATTGCCGGAGAATATTCAGTTTGAGAAGATTAAGGCTGAGGTTAAAGATGGGGTTTTGTATGTCACTATTCCTATAGCTATGAGCAGCACCAAGATTTTGGACATTAATGTTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

235

Amino Acids

26.47

Weight (kDa)

7.82

Isoelectric Point (pI)

56.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 127 - 225 2.2e-22 Hsp20/alpha crystallin family
ArsA_HSP20 PF17886 133 - 218 2.4e-07 HSP20-like domain found in ArsA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015604)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g24850
malus_domestica MD13G1108500.v1.1 MD16G1108600.v1.1
prunus_persica Prupe.1G237800_v2.0.a1
pyrus_communis pycom13g09400 pycom16g09270
rosa_chinensis RchiOBHm_Chr4g0431921
rosa_laevigata RLG00000006875
rosa_multiflora Rmu_sc0004965.1_g000008
rosa_roxburghii Rroxscaffold_5G00373350
rosa_rugosa Rorug04G0253000
rosa_samantha Rh4AG310300 Rh4BG317400 Rh4CG332700 Rh4DG312300
rosa_wichuraiana Rw4G027000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 296
AccB7I CCANNNNNTGG 1 cut(s) 77
AciI CCGC 6 cut(s) 178, 209, 293, 329, 351, 494
AfaI GTAC 2 cut(s) 356, 404
AfiI CCNNNNNNNGG 3 cut(s) 77, 215, 276
AgeI ACCGGT 1 cut(s) 228
AgsI TTSAA 2 cut(s) 97, 701
AluBI AGCT 4 cut(s) 11, 93, 392, 665
AluI AGCT 4 cut(s) 11, 93, 392, 665
Alw26I GTCTC 3 cut(s) 114, 260, 338
AoxI GGCC 1 cut(s) 99
ApeKI GCWGC 1 cut(s) 672
ArsI GACNNNNNNTTYG 2 cut(s) 429, 461
AseI ATTAAT 1 cut(s) 693
AsiGI ACCGGT 1 cut(s) 228
AspS9I GGNCC 1 cut(s) 226
AsuC2I CCSGG 1 cut(s) 426
AsuHPI GGTGA 2 cut(s) 139, 551
AvaII GGWCC 1 cut(s) 226
BanII GRGCYC 1 cut(s) 328
BbvCI CCTCAGC 1 cut(s) 624
BbvI GCAGC 1 cut(s) 684
BccI CCATC 4 cut(s) 256, 277, 503, 629
BceAI ACGGC 1 cut(s) 316
BcnI CCSGG 1 cut(s) 426
BcoDI GTCTC 3 cut(s) 114, 260, 338
BfmI CTRYAG 2 cut(s) 213, 660
BisI GCNGC 2 cut(s) 178, 673
BlsI GCNGC 2 cut(s) 179, 674
Bme1390I CCNGG 1 cut(s) 426
Bme18I GGWCC 1 cut(s) 226
BmgT120I GGNCC 1 cut(s) 226
BmiI GGNNCC 2 cut(s) 227, 327
BmrFI CCNGG 1 cut(s) 426
BplI GAGNNNNNCTC 2 cut(s) 111, 143
Bpu10I CCTNAGC 1 cut(s) 624
BpuMI CCSGG 1 cut(s) 426
BsaI GGTCTC 1 cut(s) 260
BsaJI CCNNGG 3 cut(s) 102, 270, 374
BsaWI WCCGGW 1 cut(s) 228
Bsc4I CCNNNNNNNGG 3 cut(s) 77, 215, 276
Bse118I RCCGGY 1 cut(s) 228
Bse1I ACTGG 1 cut(s) 352
Bse3DI GCAATG 1 cut(s) 587
BseDI CCNNGG 3 cut(s) 102, 270, 374
BseGI GGATG 2 cut(s) 285, 448
BseLI CCNNNNNNNGG 3 cut(s) 77, 215, 276
BseMI GCAATG 1 cut(s) 587
BseMII CTCAG 2 cut(s) 477, 615
BseNI ACTGG 1 cut(s) 352
BseRI GAGGAG 1 cut(s) 476
BseXI GCAGC 1 cut(s) 684
Bsh1285I CGRYCG 1 cut(s) 342
BshFI GGCC 1 cut(s) 101
BshTI ACCGGT 1 cut(s) 228
BsiEI CGRYCG 1 cut(s) 342
BsiSI CCGG 3 cut(s) 229, 426, 593
BslFI GGGAC 1 cut(s) 239
BslI CCNNNNNNNGG 3 cut(s) 77, 215, 276
BsmAI GTCTC 3 cut(s) 114, 260, 338
BsmFI GGGAC 1 cut(s) 239
BsnI GGCC 1 cut(s) 101
Bso31I GGTCTC 1 cut(s) 260
Bsp1286I GDGCHC 1 cut(s) 328
Bsp143I GATC 1 cut(s) 381
Bsp19I CCATGG 2 cut(s) 102, 270
BspACI CCGC 6 cut(s) 178, 209, 293, 329, 351, 494
BspANI GGCC 1 cut(s) 101
BspCNI CTCAG 2 cut(s) 478, 616
BspLI GGNNCC 2 cut(s) 227, 327
BspTNI GGTCTC 1 cut(s) 260
BsrDI GCAATG 1 cut(s) 587
BsrFI RCCGGY 1 cut(s) 228
BsrI ACTGG 1 cut(s) 352
BssAI RCCGGY 1 cut(s) 228
BssECI CCNNGG 3 cut(s) 102, 270, 374
BssMI GATC 1 cut(s) 381
BssT1I CCWWGG 2 cut(s) 102, 270
Bst4CI ACNGT 2 cut(s) 250, 375
Bst6I CTCTTC 3 cut(s) 72, 185, 537
BstDEI CTNAG 3 cut(s) 486, 555, 624
BstDSI CCRYGG 3 cut(s) 102, 270, 374
BstF5I GGATG 2 cut(s) 285, 448
BstKTI GATC 1 cut(s) 384
BstMAI GTCTC 3 cut(s) 114, 260, 338
BstMBI GATC 1 cut(s) 381
BstMCI CGRYCG 1 cut(s) 342
BstMWI GCNNNNNNNGC 3 cut(s) 113, 357, 491
BstNSI RCATGY 1 cut(s) 424
BstSCI CCNGG 1 cut(s) 424
BstSFI CTRYAG 2 cut(s) 213, 660
BstV1I GCAGC 1 cut(s) 684
BstXI CCANNNNNNTGG 1 cut(s) 685
BsuRI GGCC 1 cut(s) 101
BtgI CCRYGG 3 cut(s) 102, 270, 374
BtsCI GGATG 2 cut(s) 285, 448
Cfr10I RCCGGY 1 cut(s) 228
Cfr13I GGNCC 1 cut(s) 226
Csp6I GTAC 2 cut(s) 355, 403
CspAI ACCGGT 1 cut(s) 228
CviAII CATG 3 cut(s) 103, 271, 421
CviQI GTAC 2 cut(s) 355, 403
DdeI CTNAG 3 cut(s) 486, 555, 624
DpnI GATC 1 cut(s) 383
DpnII GATC 1 cut(s) 381
DrdI GACNNNNNNGTC 1 cut(s) 296
DseDI GACNNNNNNGTC 1 cut(s) 296
Eam1104I CTCTTC 3 cut(s) 72, 185, 537
EarI CTCTTC 3 cut(s) 72, 185, 537
EciI GGCGGA 1 cut(s) 318
Eco130I CCWWGG 2 cut(s) 102, 270
Eco24I GRGCYC 1 cut(s) 328
Eco31I GGTCTC 1 cut(s) 260
Eco47I GGWCC 1 cut(s) 226
EcoT14I CCWWGG 2 cut(s) 102, 270
EcoT38I GRGCYC 1 cut(s) 328
ErhI CCWWGG 2 cut(s) 102, 270
FaeI CATG 3 cut(s) 106, 274, 424
FaiI YATR 9 cut(s) 104, 223, 272, 422, 564, 573, 648, 662, 668
FaqI GGGAC 1 cut(s) 239
FatI CATG 3 cut(s) 102, 270, 420
FauI CCCGC 3 cut(s) 216, 300, 344
Fnu4HI GCNGC 2 cut(s) 178, 673
FokI GGATG 2 cut(s) 292, 455
FriOI GRGCYC 1 cut(s) 328
Fsp4HI GCNGC 2 cut(s) 178, 673
GluI GCNGC 2 cut(s) 178, 673
HaeIII GGCC 1 cut(s) 101
HapII CCGG 3 cut(s) 229, 426, 593
Hin1II CATG 3 cut(s) 106, 274, 424
HinfI GANTC 3 cut(s) 144, 195, 519
HpaII CCGG 3 cut(s) 229, 426, 593
HphI GGTGA 2 cut(s) 139, 551
Hpy188III TCNNGA 2 cut(s) 448, 499
HpyAV CCTTC 4 cut(s) 91, 360, 484, 530
HpyCH4III ACNGT 2 cut(s) 250, 375
HpyCH4V TGCA 3 cut(s) 139, 242, 587
HpyF10VI GCNNNNNNNGC 3 cut(s) 113, 357, 491
HpyF3I CTNAG 3 cut(s) 486, 555, 624
Hsp92II CATG 3 cut(s) 106, 274, 424
Kzo9I GATC 1 cut(s) 381
LmnI GCTCC 1 cut(s) 331
LpnPI CCDG 8 cut(s) 130, 225, 242, 333, 346, 439, 458, 606
Lsp1109I GCAGC 1 cut(s) 684
MaeIII GTNAC 3 cut(s) 3, 145, 649
MalI GATC 1 cut(s) 383
MboI GATC 1 cut(s) 381
MboII GAAGA 8 cut(s) 59, 66, 202, 539, 542, 545, 554, 625
MhlI GDGCHC 1 cut(s) 328
MluCI AATT 2 cut(s) 19, 582
MlyI GAGTC 3 cut(s) 153, 204, 528
MmeI TCCRAC 1 cut(s) 260
MnlI CCTC 9 cut(s) 38, 77, 137, 270, 405, 433, 454, 517, 619
MseI TTAA 4 cut(s) 480, 618, 630, 693
MspI CCGG 3 cut(s) 229, 426, 593
MspR9I CCNGG 1 cut(s) 426
MwoI GCNNNNNNNGC 3 cut(s) 113, 357, 491
NciI CCSGG 1 cut(s) 426
NcoI CCATGG 2 cut(s) 102, 270
NdeII GATC 1 cut(s) 381
NlaIII CATG 3 cut(s) 106, 274, 424
NlaIV GGNNCC 2 cut(s) 227, 327
NmuCI GTSAC 3 cut(s) 3, 145, 649
NspI RCATGY 1 cut(s) 424
PflMI CCANNNNNTGG 1 cut(s) 77
PinAI ACCGGT 1 cut(s) 228
PkrI GCNGC 2 cut(s) 179, 674
PleI GAGTC 3 cut(s) 152, 203, 527
PpsI GAGTC 3 cut(s) 152, 203, 527
PshBI ATTAAT 1 cut(s) 693
PspN4I GGNNCC 2 cut(s) 227, 327
PspPI GGNCC 1 cut(s) 226
RsaI GTAC 2 cut(s) 356, 404
RsaNI GTAC 2 cut(s) 355, 403
SaqAI TTAA 4 cut(s) 480, 618, 630, 693
SatI GCNGC 2 cut(s) 178, 673
Sau3AI GATC 1 cut(s) 381
Sau96I GGNCC 1 cut(s) 226
SchI GAGTC 3 cut(s) 153, 204, 528
ScrFI CCNGG 1 cut(s) 426
SduI GDGCHC 1 cut(s) 328
SetI ASST 9 cut(s) 13, 88, 95, 129, 394, 416, 541, 630, 667
SfcI CTRYAG 2 cut(s) 213, 660
SinI GGWCC 1 cut(s) 226
Sse9I AATT 2 cut(s) 19, 582
SsiI CCGC 6 cut(s) 178, 209, 293, 329, 351, 494
SspI AATATT 1 cut(s) 601
StyD4I CCNGG 1 cut(s) 424
StyI CCWWGG 2 cut(s) 102, 270
TaaI ACNGT 2 cut(s) 250, 375
TaqI TCGA 3 cut(s) 129, 417, 522
TaqII GACCGA 1 cut(s) 356
TasI AATT 2 cut(s) 19, 582
TatI WGTACW 1 cut(s) 402
TauI GCSGC 1 cut(s) 180
Tru1I TTAA 4 cut(s) 480, 618, 630, 693
Tru9I TTAA 4 cut(s) 480, 618, 630, 693
TseFI GTSAC 3 cut(s) 3, 145, 649
TseI GCWGC 1 cut(s) 672
Tsp45I GTSAC 3 cut(s) 3, 145, 649
Van91I CCANNNNNTGG 1 cut(s) 77
VpaK11BI GGWCC 1 cut(s) 226
VspI ATTAAT 1 cut(s) 693
XceI RCATGY 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.