Rorug04G0253000

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0011089
Physical Location & Seq
Reverse (-)
42590977 .. 42595831
4855 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0253000.1

Sequence Viewer

Length: 1071 bp
ATGGAGAGGTACGGTCGGAACACGGAAGGGACGCAGTCGGATCCGTCGCCGGAGTGGAGCGGTCCGGGACCGGAAACCGGGCTGGAAGAATCCATGTGGCAGTTGGGCCTCGGACCCGGAGAATCGTACCCGGAACGGCCCGAGGAGGCCGATTGCAGTTACTATCTCAGGACTGGGATTTGTGGCTACGGCTCGAGGTGTCGGTACAATCACCCCCGTGATCGTACTGCGGTTACCGGAGCTCTGAGAGCTGGGGCGGTGGAGTACCCCGAGCGACTGGGCCAGCCTGTGTGCCAGTATTATATGAGGACAGGGACGTGCAAATTTGGCGCCACTTGTAAGTATCACCATCCTAAGCAGGGAGGAGGCTCTGCGGCCCCTGTATCACTAAATTACTATGGATACCCATTACGACCGGGTGAAAGAGAGTGTTCCTACTATGTGAAAACTGGACAGTGTAAGTTTGGTGCAACTTGTAAATTCCATCATCCACAGCTGACTGGCACACAATCTCAAGTGCCATCACAAGCCCAAGCTTCAATTATACCTGCACCCACATTGTATCAAACTGTGCAAACTCCATCCGTTTCCTCACAGCCCTATGGAACAGTAATGGTTGCAAGGCCTCCTTTGCTACCTAGCTCATACGTTCAAAGCCCCTACGGTCCAATGCTGATTCCCCCTGGAATGGTTCCTTTCTCAGGTTGGAGTCCATATCCGGCTGGTGCAAGCCCATTAGCGTCTCCTAGTACTCAAACCGCTGTTGGTTCAGGGGCACTTTATGGGATAAATCAACTATCTCCTTCAGCGCCTGCATATACAGGAATCTTTCCAACTGTACCTTTTTCTCTTGGTCCTCCAACCTCTAGCCAGAAGGAGCAATCTCATCCTGAAAGACCTGGCCAAGCAGAATGTCAGTATTACCTGAGAACAGGGGATTGTAAATTTGGGTCCTCATGTAGATATCATCATCCAGCAGAAGTTGTTGCACCAAATACACCTGTTGTCCTTAGCTCCATTGGTCTTCCGTCGCGCCCGGTGAGCCGCTCTTTCAACTCCTCTGCATCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

356

Amino Acids

38.29

Weight (kDa)

8.52

Isoelectric Point (pI)

57.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCCH PF00642 48 - 73 1.7e-09 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
zf-CCCH PF00642 94 - 119 7.6e-11 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
zf-CCCH PF00642 139 - 165 1.2e-09 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
zf-CCCH PF00642 301 - 325 2e-09 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011089)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67700 AT1G67700 AT1G67700 AT1G67700 AT1G67700
fragaria_vesca FvH4_4g24830
malus_domestica MD13G1108600.v1.1
prunus_persica Prupe.1G237600_v2.0.a1
pyrus_communis pycom13g09410
rosa_chinensis RchiOBHm_Chr4g0431911
rosa_laevigata RLG00000006876
rosa_multiflora Rmu_sc0004965.1_g000007
rosa_roxburghii Rroxscaffold_5G00373340
rosa_rugosa Rorug04G0253000 Rorug04G0253000
rosa_samantha Rh4AG310200 Rh4BG317300 Rh4CG332600 Rh4DG312200
rosa_wichuraiana Rw4G026990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 556
AccB1I GGYRCC 1 cut(s) 329
AccBSI CCGCTC 2 cut(s) 60, 1047
AccII CGCG 1 cut(s) 1033
AciI CCGC 6 cut(s) 60, 230, 257, 374, 759, 1045
AclWI GGATC 2 cut(s) 35, 48
AcoI YGGCCR 1 cut(s) 901
AcsI RAATTY 3 cut(s) 323, 479, 944
AcuI CTGAAG 1 cut(s) 789
AcyI GRCGYC 1 cut(s) 330
AfaI GTAC 7 cut(s) 11, 128, 206, 226, 266, 751, 840
AfiI CCNNNNNNNGG 4 cut(s) 77, 359, 688, 701
AgsI TTSAA 3 cut(s) 540, 653, 1054
AjiI CACGTC 1 cut(s) 318
AjnI CCWGG 2 cut(s) 682, 898
AleI CACNNNNGTG 1 cut(s) 216
AluBI AGCT 6 cut(s) 242, 251, 496, 536, 642, 1014
AluI AGCT 6 cut(s) 242, 251, 496, 536, 642, 1014
Alw21I GWGCWC 1 cut(s) 244
Alw26I GTCTC 1 cut(s) 747
AlwI GGATC 2 cut(s) 35, 48
AlwNI CAGNNNCTG 1 cut(s) 812
Ama87I CYCGRG 3 cut(s) 140, 193, 269
AoxI GGCC 7 cut(s) 106, 137, 147, 280, 375, 623, 901
ApoI RAATTY 3 cut(s) 323, 479, 944
AspLEI GCGC 3 cut(s) 332, 811, 1035
AsuC2I CCSGG 6 cut(s) 66, 79, 117, 131, 417, 1037
AsuHPI GGTGA 4 cut(s) 203, 338, 431, 1051
AvaI CYCGRG 3 cut(s) 140, 193, 269
AvaII GGWCC 6 cut(s) 62, 68, 113, 665, 854, 951
BaeGI GKGCMC 1 cut(s) 778
BalI TGGCCA 1 cut(s) 903
BamHI GGATCC 1 cut(s) 40
BanI GGYRCC 1 cut(s) 329
BanII GRGCYC 1 cut(s) 244
BbsI GAAGAC 1 cut(s) 1016
Bbv12I GWGCWC 1 cut(s) 244
BccI CCATC 4 cut(s) 357, 492, 529, 589
BceAI ACGGC 2 cut(s) 152, 205
BciT130I CCWGG 2 cut(s) 684, 900
BciVI GTATCC 1 cut(s) 395
BcnI CCSGG 6 cut(s) 66, 79, 117, 131, 417, 1037
BcoDI GTCTC 1 cut(s) 747
BfaI CTAG 3 cut(s) 639, 747, 867
BfoI RGCGCY 2 cut(s) 333, 812
BfuAI ACCTGC 1 cut(s) 556
BfuI GTATCC 1 cut(s) 395
BisI GCNGC 2 cut(s) 375, 1045
BlsI GCNGC 2 cut(s) 376, 1046
BmcAI AGTACT 1 cut(s) 751
Bme1390I CCNGG 8 cut(s) 66, 79, 117, 131, 417, 684, 900, 1037
Bme18I GGWCC 6 cut(s) 62, 68, 113, 665, 854, 951
BmeT110I CYCGRG 3 cut(s) 140, 193, 269
BmgBI CACGTC 1 cut(s) 318
BmiI GGNNCC 7 cut(s) 42, 69, 115, 331, 378, 693, 952
BmrFI CCNGG 8 cut(s) 66, 79, 117, 131, 417, 684, 900, 1037
BmrI ACTGGG 2 cut(s) 183, 287
BmuI ACTGGG 2 cut(s) 183, 287
BpiI GAAGAC 1 cut(s) 1016
Bpu10I CCTNAGC 2 cut(s) 354, 1010
BpuEI CTTGAG 1 cut(s) 498
BpuMI CCSGG 6 cut(s) 66, 79, 117, 131, 417, 1037
BsaHI GRCGYC 1 cut(s) 330
BsaJI CCNNGG 3 cut(s) 109, 141, 682
BsaWI WCCGGW 2 cut(s) 70, 236
Bsc4I CCNNNNNNNGG 4 cut(s) 77, 359, 688, 701
Bse1I ACTGG 5 cut(s) 178, 282, 295, 454, 505
BseBI CCWGG 2 cut(s) 684, 900
BseDI CCNNGG 3 cut(s) 109, 141, 682
BseGI GGATG 5 cut(s) 349, 487, 581, 886, 970
BseLI CCNNNNNNNGG 4 cut(s) 77, 359, 688, 701
BseMII CTCAG 4 cut(s) 181, 236, 714, 917
BseNI ACTGG 5 cut(s) 178, 282, 295, 454, 505
BseRI GAGGAG 3 cut(s) 158, 378, 1048
BseSI GKGCMC 1 cut(s) 778
BseYI CCCAGC 1 cut(s) 251
BsgI GTGCAG 1 cut(s) 534
Bsh1236I CGCG 1 cut(s) 1033
Bsh1285I CGRYCG 2 cut(s) 16, 416
BshFI GGCC 7 cut(s) 108, 139, 149, 282, 377, 625, 903
BshNI GGYRCC 1 cut(s) 329
BsiEI CGRYCG 2 cut(s) 16, 416
BsiHKAI GWGCWC 1 cut(s) 244
BsiHKCI CYCGRG 3 cut(s) 140, 193, 269
BslFI GGGAC 3 cut(s) 43, 81, 328
BslI CCNNNNNNNGG 4 cut(s) 77, 359, 688, 701
BsmAI GTCTC 1 cut(s) 747
BsmBI CGTCTC 1 cut(s) 747
BsmFI GGGAC 3 cut(s) 43, 81, 328
BsnI GGCC 7 cut(s) 108, 139, 149, 282, 377, 625, 903
BsoBI CYCGRG 3 cut(s) 140, 193, 269
Bsp1286I GDGCHC 2 cut(s) 244, 778
Bsp143I GATC 2 cut(s) 40, 220
BspACI CCGC 6 cut(s) 60, 230, 257, 374, 759, 1045
BspANI GGCC 7 cut(s) 108, 139, 149, 282, 377, 625, 903
BspCNI CTCAG 4 cut(s) 180, 237, 713, 918
BspFNI CGCG 1 cut(s) 1033
BspLI GGNNCC 7 cut(s) 42, 69, 115, 331, 378, 693, 952
BspMI ACCTGC 1 cut(s) 556
BspPI GGATC 2 cut(s) 35, 48
BspT107I GGYRCC 1 cut(s) 329
BsrBI CCGCTC 2 cut(s) 60, 1047
BsrI ACTGG 5 cut(s) 178, 282, 295, 454, 505
BssECI CCNNGG 3 cut(s) 109, 141, 682
BssMI GATC 2 cut(s) 40, 220
BssNI GRCGYC 1 cut(s) 330
Bst2UI CCWGG 2 cut(s) 684, 900
Bst4CI ACNGT 6 cut(s) 14, 456, 571, 610, 665, 838
BstACI GRCGYC 1 cut(s) 330
BstC8I GCNNGC 3 cut(s) 284, 730, 813
BstDEI CTNAG 6 cut(s) 167, 245, 354, 700, 926, 1010
BstEII GGTNACC 1 cut(s) 232
BstENI CCTNNNNNAGG 1 cut(s) 699
BstF5I GGATG 5 cut(s) 349, 487, 581, 886, 970
BstFNI CGCG 1 cut(s) 1033
BstH2I RGCGCY 2 cut(s) 333, 812
BstHHI GCGC 3 cut(s) 332, 811, 1035
BstKTI GATC 2 cut(s) 43, 223
BstMAI GTCTC 1 cut(s) 747
BstMBI GATC 2 cut(s) 40, 220
BstMCI CGRYCG 2 cut(s) 16, 416
BstMWI GCNNNNNNNGC 4 cut(s) 248, 327, 631, 1041
BstNI CCWGG 2 cut(s) 684, 900
BstPI GGTNACC 1 cut(s) 232
BstSCI CCNGG 8 cut(s) 64, 77, 115, 129, 415, 682, 898, 1035
BstSLI GKGCMC 1 cut(s) 778
BstUI CGCG 1 cut(s) 1033
BstV2I GAAGAC 1 cut(s) 1016
BstX2I RGATCY 1 cut(s) 40
BstYI RGATCY 1 cut(s) 40
BsuI GTATCC 1 cut(s) 395
BsuRI GGCC 7 cut(s) 108, 139, 149, 282, 377, 625, 903
BtrI CACGTC 1 cut(s) 318
BtsCI GGATG 5 cut(s) 349, 487, 581, 886, 970
BtsIMutI CAGTG 1 cut(s) 461
BveI ACCTGC 1 cut(s) 556
Cac8I GCNNGC 3 cut(s) 284, 730, 813
CaiI CAGNNNCTG 1 cut(s) 812
CfoI GCGC 3 cut(s) 332, 811, 1035
CpoI CGGWCCG 1 cut(s) 62
CseI GACGC 2 cut(s) 40, 729
Csp6I GTAC 7 cut(s) 10, 127, 205, 225, 265, 750, 839
CspI CGGWCCG 1 cut(s) 62
CviAII CATG 2 cut(s) 94, 957
CviQI GTAC 7 cut(s) 10, 127, 205, 225, 265, 750, 839
DdeI CTNAG 6 cut(s) 167, 245, 354, 700, 926, 1010
DinI GGCGCC 1 cut(s) 331
DpnI GATC 2 cut(s) 42, 222
DpnII GATC 2 cut(s) 40, 220
EaeI YGGCCR 1 cut(s) 901
Ecl136II GAGCTC 1 cut(s) 242
Eco147I AGGCCT 1 cut(s) 625
Eco24I GRGCYC 1 cut(s) 244
Eco32I GATATC 1 cut(s) 965
Eco47I GGWCC 6 cut(s) 62, 68, 113, 665, 854, 951
Eco53kI GAGCTC 1 cut(s) 242
Eco57I CTGAAG 1 cut(s) 789
Eco88I CYCGRG 3 cut(s) 140, 193, 269
Eco91I GGTNACC 1 cut(s) 232
EcoICRI GAGCTC 1 cut(s) 242
EcoNI CCTNNNNNAGG 1 cut(s) 699
EcoO109I RGGNCCY 1 cut(s) 951
EcoO65I GGTNACC 1 cut(s) 232
EcoRII CCWGG 2 cut(s) 682, 898
EcoRV GATATC 1 cut(s) 965
EcoT38I GRGCYC 1 cut(s) 244
EgeI GGCGCC 1 cut(s) 331
EheI GGCGCC 1 cut(s) 331
Esp3I CGTCTC 1 cut(s) 747
FaeI CATG 2 cut(s) 97, 960
FalI AAGNNNNNCTT 2 cut(s) 613, 645
FaqI GGGAC 3 cut(s) 43, 81, 328
FatI CATG 2 cut(s) 93, 956
Fnu4HI GCNGC 2 cut(s) 375, 1045
FokI GGATG 5 cut(s) 336, 474, 568, 873, 957
FriOI GRGCYC 1 cut(s) 244
Fsp4HI GCNGC 2 cut(s) 375, 1045
FspBI CTAG 3 cut(s) 639, 747, 867
GlaI GCGC 3 cut(s) 331, 810, 1034
GluI GCNGC 2 cut(s) 375, 1045
GsaI CCCAGC 1 cut(s) 255
HaeII RGCGCY 2 cut(s) 333, 812
HaeIII GGCC 7 cut(s) 108, 139, 149, 282, 377, 625, 903
HgaI GACGC 2 cut(s) 40, 729
HhaI GCGC 3 cut(s) 332, 811, 1035
Hin1I GRCGYC 1 cut(s) 330
Hin1II CATG 2 cut(s) 97, 960
Hin6I GCGC 3 cut(s) 330, 809, 1033
HinP1I GCGC 3 cut(s) 330, 809, 1033
HindIII AAGCTT 1 cut(s) 534
HinfI GANTC 5 cut(s) 89, 122, 676, 709, 825
HphI GGTGA 4 cut(s) 203, 338, 431, 1051
Hpy188I TCNGA 4 cut(s) 18, 40, 113, 246
Hpy188III TCNNGA 3 cut(s) 169, 890, 1068
Hpy99I CGWCG 2 cut(s) 49, 1033
HpyAV CCTTC 3 cut(s) 20, 813, 868
HpyCH4III ACNGT 6 cut(s) 14, 456, 571, 610, 665, 838
HpyCH4IV ACGT 2 cut(s) 317, 648
HpyF10VI GCNNNNNNNGC 4 cut(s) 248, 327, 631, 1041
HpyF3I CTNAG 6 cut(s) 167, 245, 354, 700, 926, 1010
HpySE526I ACGT 2 cut(s) 317, 648
Hsp92I GRCGYC 1 cut(s) 330
Hsp92II CATG 2 cut(s) 97, 960
HspAI GCGC 3 cut(s) 330, 809, 1033
KasI GGCGCC 1 cut(s) 329
Kzo9I GATC 2 cut(s) 40, 220
LmnI GCTCC 4 cut(s) 57, 239, 877, 1019
MaeI CTAG 3 cut(s) 639, 747, 867
MaeII ACGT 2 cut(s) 317, 648
MaeIII GTNAC 2 cut(s) 158, 232
MalI GATC 2 cut(s) 42, 222
MbiI CCGCTC 2 cut(s) 60, 1047
MboI GATC 2 cut(s) 40, 220
MboII GAAGA 2 cut(s) 98, 1016
MflI RGATCY 1 cut(s) 40
MhlI GDGCHC 2 cut(s) 244, 778
MlsI TGGCCA 1 cut(s) 903
MluCI AATT 5 cut(s) 323, 391, 479, 540, 944
MluNI TGGCCA 1 cut(s) 903
Mly113I GGCGCC 1 cut(s) 330
MlyI GAGTC 1 cut(s) 718
MmeI TCCRAC 4 cut(s) 18, 686, 857, 884
Mox20I TGGCCA 1 cut(s) 903
MscI TGGCCA 1 cut(s) 903
MslI CAYNNNNRTG 1 cut(s) 216
Msp20I TGGCCA 1 cut(s) 903
MspA1I CMGCKG 2 cut(s) 496, 761
MspR9I CCNGG 8 cut(s) 66, 79, 117, 131, 417, 684, 900, 1037
MvaI CCWGG 2 cut(s) 684, 900
MvnI CGCG 1 cut(s) 1033
MwoI GCNNNNNNNGC 4 cut(s) 248, 327, 631, 1041
NarI GGCGCC 1 cut(s) 330
NciI CCSGG 6 cut(s) 66, 79, 117, 131, 417, 1037
NdeII GATC 2 cut(s) 40, 220
NlaIII CATG 2 cut(s) 97, 960
NlaIV GGNNCC 7 cut(s) 42, 69, 115, 331, 378, 693, 952
OliI CACNNNNGTG 1 cut(s) 216
PaeR7I CTCGAG 1 cut(s) 193
PceI AGGCCT 1 cut(s) 625
PfeI GAWTC 4 cut(s) 89, 122, 676, 825
PflFI GACNNNGTC 1 cut(s) 34
PfoI TCCNGGA 1 cut(s) 64
PkrI GCNGC 2 cut(s) 376, 1046
PleI GAGTC 1 cut(s) 717
PluTI GGCGCC 1 cut(s) 333
PpsI GAGTC 1 cut(s) 717
PpuMI RGGWCCY 1 cut(s) 951
Psp124BI GAGCTC 1 cut(s) 244
Psp5II RGGWCCY 1 cut(s) 951
Psp6I CCWGG 2 cut(s) 682, 898
PspEI GGTNACC 1 cut(s) 232
PspFI CCCAGC 1 cut(s) 251
PspGI CCWGG 2 cut(s) 682, 898
PspN4I GGNNCC 7 cut(s) 42, 69, 115, 331, 378, 693, 952
PspPPI RGGWCCY 1 cut(s) 951
PspXI VCTCGAGB 1 cut(s) 193
PstNI CAGNNNCTG 1 cut(s) 812
PsuI RGATCY 1 cut(s) 40
PsyI GACNNNGTC 1 cut(s) 34
PvuII CAGCTG 1 cut(s) 496
RsaI GTAC 7 cut(s) 11, 128, 206, 226, 266, 751, 840
RsaNI GTAC 7 cut(s) 10, 127, 205, 225, 265, 750, 839
RseI CAYNNNNRTG 1 cut(s) 216
Rsr2I CGGWCCG 1 cut(s) 62
RsrII CGGWCCG 1 cut(s) 62
SacI GAGCTC 1 cut(s) 244
SatI GCNGC 2 cut(s) 375, 1045
Sau3AI GATC 2 cut(s) 40, 220
ScaI AGTACT 1 cut(s) 751
SchI GAGTC 1 cut(s) 718
ScrFI CCNGG 8 cut(s) 66, 79, 117, 131, 417, 684, 900, 1037
SduI GDGCHC 2 cut(s) 244, 778
SfoI GGCGCC 1 cut(s) 331
Sfr274I CTCGAG 1 cut(s) 193
SinI GGWCC 6 cut(s) 62, 68, 113, 665, 854, 951
SlaI CTCGAG 1 cut(s) 193
SmiMI CAYNNNNRTG 1 cut(s) 216
SmlI CTYRAG 2 cut(s) 193, 513
SmoI CTYRAG 2 cut(s) 193, 513
Sse9I AATT 5 cut(s) 323, 391, 479, 540, 944
SseBI AGGCCT 1 cut(s) 625
SsiI CCGC 6 cut(s) 60, 230, 257, 374, 759, 1045
SspDI GGCGCC 1 cut(s) 329
SspMI CTAG 3 cut(s) 639, 747, 867
SstI GAGCTC 1 cut(s) 244
StuI AGGCCT 1 cut(s) 625
StyD4I CCNGG 8 cut(s) 64, 77, 115, 129, 415, 682, 898, 1035
TaaI ACNGT 6 cut(s) 14, 456, 571, 610, 665, 838
TaiI ACGT 2 cut(s) 320, 651
TaqI TCGA 1 cut(s) 194
TasI AATT 5 cut(s) 323, 391, 479, 540, 944
TatI WGTACW 1 cut(s) 749
TauI GCSGC 2 cut(s) 377, 1047
TfiI GAWTC 4 cut(s) 89, 122, 676, 825
TscAI CASTG 1 cut(s) 461
TspGWI ACGGA 4 cut(s) 33, 38, 574, 1017
TspRI CASTG 1 cut(s) 461
Tth111I GACNNNGTC 1 cut(s) 34
VpaK11BI GGWCC 6 cut(s) 62, 68, 113, 665, 854, 951
XagI CCTNNNNNAGG 1 cut(s) 699
XapI RAATTY 3 cut(s) 323, 479, 944
XcmI CCANNNNNNNNNTGG 1 cut(s) 100
XhoI CTCGAG 1 cut(s) 193
XspI CTAG 3 cut(s) 639, 747, 867
ZrmI AGTACT 1 cut(s) 751
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.